The invention belongs to the field of microbial
fermentation and
biopolymer modification, and particularly relates to a preparation method of cross-linked
polyglutamic acid and application of the cross-linked
polyglutamic acid in microalgae harvesting. The preparation method comprises the following steps: by taking
polyglutamic acid fermentation liquor subjected to sterilization,
salting-out and redissolving treatment or a solution prepared from polyglutamic acid
powder as a
raw material, adjusting the pH value to be within a proper range, adding
ethanol into the polyglutamic acid
fermentation liquor or the solution prepared from the polyglutamic acid
powder in proportion, uniformly stirring, collecting pasty precipitate, and weighing; and adding a cross-linking agent into the paste precipitate according to a proper
mass ratio of the cross-linking agent to the polyglutamic acid, uniformly mixing, spreading in a
drying tray,
drying in
vacuum drying equipment at a certain temperature to constant weight, crushing by using a
crusher, and sieving by using a 40-mesh
sieve to obtain a cross-linked polyglutamic acid product. The invention provides another preparation method of the cross-linked polyglutamic acid, the cross-linking reaction and the
drying process are fused, the
ethanol is used as an
accelerant of the reaction, the dosage of the cross-linking agent is small, the product yield is high, the preparation cost is low, and the preparation method is suitable for large-scale production.