The application provides a silk
fibroin microsphere and a preparation method and application thereof, and belongs to the technical field of
biopolymer microsphere preparation. The preparation method comprises the following steps: 1) adding an ionic dispersing agent into a silk
fibroin solution, uniformly mixing, then adding a sphere forming agent into the solution, and obtaining a pretreated liquid after mixing; 2) performing low-temperature coagulation treatment on the pretreated liquid, and obtaining a silk
fibroin microsphere solution after thawing; 3) diluting the silk fibroin microsphere solution, then performing wet heat
crystallization treatment, and obtaining a high-
crystallinity silk fibroin microsphere solution; and 4) performing
spray drying on the high-
crystallinity silk fibroin microsphere solution, and obtaining a
solid silk fibroin microsphere. The silk fibroin microsphere obtained by the application has excellent
biocompatibility, degradability and good mechanical properties. The silk fibroin microsphere prepared by the method of the application is regular spherical, has high
crystallinity, slow degradation speed, is not easy to absorb
moisture and adhere, and can be stored for a long time; and the preparation method is simple and has high yield.