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400 results about "Matrix solution" patented technology

Hot pepper and determining method for 96 pesticide residues in product of hot pepper

The invention discloses hot pepper and a determining method for 96 pesticide residues in a product of the hot pepper. The determining method comprises the following steps: homogenously extracting residual pesticide in a sample with 1% acetic acid-acetonitrile solution, purifying the extracting solution with a Florisil solid phase extraction column, dispersing and purifying the extracting solution with ethylenediamine-N-propyl silane (PSA) and octadecyl silane bonded phase (C18) substrate, detecting 69 pesticide residuals in the purified concentrated liquid of the Florisil column by GC-MS (gaschromatographic mass spectrometry), detecting 27 pesticide residuals in the substrate dispersed purified liquid by liquid chromatography-tandem mass spectrometry (LC-MS / MS), using the black substrate solution dilution standard to construct the updated calibration curves, adopting an internal standard method to quantify when using GC / MS to detect the residuals, and adopting an external standard method to quantify when using LC-MS / MS to detect the residuals. The average recovery rate of the method is 70.7-118.6%; the average relative standard deviation (RSD) is 3.2-11.4%; the detection limit is 0.13-28.2 ug / kg. The determining method has the advantages of simplicity and convenience in operation, high speed, accuracy, high sensitivity and good repeatability.
Owner:INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU

Detection of analytes using electrochemistry

The present invention relates to diagnostic assays whereby the detection means is based on electrochemical reactions. This means that the label to be detected provides an electric signal. Preferred labels are enzymes giving such a signal. Provided is a flow cell whereby a solid phase is provided in a flow stream of the sample, in close proximity to a working electrode to detect any electrical signal. In a typical embodiment, a sample is mixed with molecule having specific binding affinity for an analyte of which the presence in the sample is to be detected, whereby said specific binding molecule is provided with a label. The conjugate of labelled specific binding molecule and analyte is then immobilized on the solid phase in the vicinity of the working electrode, the flow cell is rinsed with a solution and afterwards a substrate solution for the label (an enzyme) is provided upon which an electrical signal is generated and can be detected by the working electrode. The methods and devices of the present invention are particular useful for liquids which comprise many substances that may disturb measurement in conventional assays. The design of the flow cell allows for removal of said interfering substances before measurement. In a preferred embodiment at least part of the solid phase is provided in the form of magnetic beads. In this embodiment the solid phase can be mixed with the sample thereby creating a longer reaction time, a better sensitivity and a higher speed of the assay.
Owner:DSM NV

Method for preparing high dielectric constant polymer-based nano composite material

InactiveCN103147226AHigh dielectric constantControllable dielectric constantNon-woven fabricsFiberMatrix solution
The invention provides a method for preparing high dielectric constant polymer-based nano composite material. The method comprises the following steps: performing electrostatic spinning on a polymer matrix solution and simultaneously performing electrostatic spraying on a filler particle suspension; simultaneously receiving the products of the electrostatic spinning and the electrostatic spraying through a common receiving device to obtain a composite fiber membrane; and finally obtaining the high dielectric constant polymer-based nano composite material with filler particles uniformly dispersed after the composite fiber membrane is processed through a hot-pressing processing technique. The composite material prepared by the invention has the characteristics of high dielectric constant, low dielectric loss and the like; and at the same time the dielectric constant of the composite material can be adjusted through adjusting the content of the filler particles in the polymer matrix. The invention has the advantages of simple process, convenient operation and little environment pollution, can well solve the problem that the filler particles is uniformly dispersed in the polymer matrix, and is expected to the microelectronic industry as dielectric material and the like for preparing embedded capacitors.
Owner:JIANGXI NORMAL UNIV

Method for preparing fusiform complicated organ precursor by using rotary assembling die

InactiveCN102871771AIncrease contact areaOvercome the disadvantages such as difficult formingProsthesisMatrix solutionPhosphate
The invention provides a method for preparing a fusiform complicated organ precursor by using a rotary assembling die, which comprises the following steps of: firstly, preparing a cellular matrix solution and a synthetic macromolecular solution, assembling the assembling die with a multilevel branching internal die, pouring the cellular matrix solution between an internal ring die and a base die, rotating the base die and carrying out physical or chemical cross-linking or polymerization to obtain a cellular matrix layer; secondly, pouring the synthetic macromolecular solution between the cellular matrix layer and an external ring die, rotating and extracting the base die with a cellular culture solution or PBS (Phosphate Buffer Solution) to form an outer support, removing the assembling die to obtain a semi-fusiform three-dimensional structure with multiple branching channels containing different cells and synthetic macromolecule supports, and permeably adhering and mixing the two semi-spindles through either natural or synthetic macromolecular solution to form the complete fusiform complicated organ precursor with the internal branching pipe. The method for preparing the fusiform complicated organ precursor by using the rotary assembling die overcomes the defects that the existing cells are difficult to penetrate into the three-dimensional supports and the supports containing multiple cells are unlikely to form and the like.
Owner:TSINGHUA UNIV

Matrix addition system for mass spectrum analysis

The invention discloses a matrix addition system for mass spectrum analysis, comprising a heating bath, a thermal saturated pipe, a condenser pipe and a peripheral control module, wherein the heating bath is used for containing a matrix solution; a first heating band is fixed outside the heating bath; the thermal saturated pipe is connected with the heating bath; a second heating band is fixed outside the thermal saturated pipe and used for providing appropriate temperature for a mixed gas of aerosol particles and a matrix so that the aerosol particles and the matrix are better bonded together; the condenser pipe is connected with the thermal saturated pipe; a refrigeration sheet is fixed outside the condenser pipe and used for condensing a gas exhausted from the thermal saturated pipe to enable the vaporous matrix, which is not attached to the aerosol particles, to be liquefied and reflow to the heating bath so as to obtain the aerosol particles attached with the matrix on an outlet of the condenser pipe; and the peripheral control module is respectively connected with the first heating band, the second heating band and the refrigeration sheet and used for regulating and displaying the set temperature and the current temperature of the heating bath, the thermal saturated pipe and the condenser pipe.
Owner:北京汇丰隆经济技术开发有限公司

Method for preparing complex organ precursor with branch vessel network

The invention provides a method for preparing complex organ precursor with a branch vessel network. The method comprises the following steps: firstly preparing one or more cellular matrix solutions and a synthetic macromolecular solution; pouring the cellular matrix solution into a combined mold in layers for physical or chemical cross-linking to form a multilayered cellular matrix layer; taking out a branch mold and removing a shell mold in layers from inwards to outwards; pouring macromolecular solutions of different types into gaps remained in the shell mold respectively so as to form a multilayered synthetic macromolecule shell; and removing the combined mold and abutting two formed structures so as to obtain the complex organ precursor. According to the method, a complex organ precursor three-dimensional structure, in which a branch vessel network is provided and cellular matrix materials of different types and synthetic macromolecule support shells are combined, is formed; and the defects that the tissue engineering is simple in three-dimensional support surface and the internal structure, low in similarity with real organs, single in cell type and uneven in cell distribution, less in possibility for survival of closed cells, difficult in non-channel differentiation vessels and strong in support appearance limitation are solved.
Owner:TSINGHUA UNIV

Traffic accident site rapid mapping system based on image guiding

A traffic accident site rapid mapping system based on image guiding comprises a homography matrix solution module, a space coordinate transformation module, a re-sampling module, a mapping and marking module and a file preserving and printing module. The homography matrix solution module builds a mapping relationship between a traffic accident site picture image plane and an actual road plane according to the two-dimensional direct linear transformation method, the space coordinate transformation module restores the position information of a traffic site picture in object space according to the homography matrix and scales the range of the traffic accident site to the size range of the original traffic accident site picture, the re-sampling module restores gray information of the traffic accident site picture in object space and achieves transformation from a perspective picture to an orthographic projection picture of the traffic accident site picture, the mapping and marking module achieves direct mapping and relevant size marking of the traffic accident site information on the orthographic projection picture, and the file preserving and printing module achieves functions of preservation and site printout of the traffic accident site picture. By means of the traffic accident site rapid mapping system, the accuracy of traffic accident site survey is improved, the on-site data processing and mapping time is saved, and the requirement of site picture printout on the traffic accident site is further satisfied.
Owner:SHANGHAI JIAO TONG UNIV SUBEI RES INST

Method and special mould for preparing tissue and organ precursor with multi-branch channels

The invention relates to a method and special mould for preparing a tissue and organ precursor with multi-branch channels, wherein the special mould comprises a base mould, at least one stage of inner mould and at least one stage of outer mould. The method provided by the invention comprises the steps that: the inner mould and the outer mould are arranged on the base mould in sequence according to the stages, when each stages of mould is mounted, a matrix solution containing cells is poured in the outer mould cavity, after the crosslinking of the matrix solution containing cells, the outer mould and the inner mould are removed, so that a layer of cell matrix layer with channels is formed; then the above steps are repeated, so that multiple layers of the cell matrix layer with channels are prepared in sequence, the interlayer channels are interconnected in branch-shaped, the multilayer cell matrix layer is penetrated and adhered through the synthetic polymer solution to form the tissue and organ precursor with multi-branch channels, and the shaped multi-branch channels can form the structures, such as blood vessel, nervus, bile duct, trachea and the like. The method provided by the invention overcome a defect that it is difficult to construct the complex branch channel in prior technology, and has advantages including that: the range of shaped tissue and organ is wide, the mounting of matrix materials containing high density of cells is easy to be realize.
Owner:TSINGHUA UNIV

Measurement method for cyenopyrafen residue amount

The invention discloses a measurement method for cyenopyrafen residue amount. The measurement method comprises the following steps: homogeneously extracting residual cyenopyrafen in a sample by using acetonitrile or 1 percent acetic acid-acetonitrile so as to obtain extracted liquid, performing LC-MS/MS (liquid chromatography-mass spectrometry/mass spectrometry) detection after the extracted liquid is dispersed and purified by a bonded phase (C18) of ethylenediamine-N-propyl silane (PSA) and an octadecylsilane and graphitized carbon (Carb) substrate; establishing a corrected standard curve by adopting a blank matrix solution dilution standard, and fixing the quantity by adopting an external standard method. According to the method, the average recovery rate is 87.6 to 94.5 percent, the average relative standard deviation (RSD) is 3.7 to 6.4 percent, the detection limit is lower than 0.42mu g/kg; the measurement method has the advantages of convenience and rapidness in operation, high sensitivity, and high repeatability, and the qualitative and quantitative accuracy is high. The technical requirements on corresponding product safety detection in countries, such as Korea, Japan, and the European Union, can be met; the powerful technical support is provided for guaranteeing the food security of Chinese people and the sustainable development on foreign export trade.
Owner:崔淑华

Preparation method of multifunctional biodegradable oil-water separation film

The invention discloses a preparation method of a multifunctional biodegradable oil-water separation film. The Preparation method of multifunctional biodegradable oil-water separation film comprises the following steps: (1) preparing a polymer matrix solution; (2) preparing a nano-fiber film by virtue of electrostatic spinning; and (3) realizing the construction of a layered micro-nano structure and the nitrogen doping of titanium dioxide once by virtue of a hydrothermal method, so as to obtain the biodegradable oil-water separation film. The prepared biodegradable oil-water separation film iscapable of effectively degrading organic dyes and efficiently separating oil and water under the irradiation of visible light, and ''two purposes'' are realized; on one hand, the organic dyes can bedegraded under the irradiation of the visible light, so that the film is environmentally friendly and low in energy consumption, and the secondary pollution problem caused due to the adsorption and desorption of adsorption materials can be avoided; and on the other hand, the oil-water separation film has biodegradability and is harmless to the environment, so that the post-processing problem of non-biodegradable materials is solved, and the film has very wide application prospects in the fields of the treatment of dye sewage and oil-containing sewage, the recycling of oil and the like.
Owner:石狮市川大先进高分子材料研究中心

Bioactivity bracket and preparation method thereof

The invention discloses a bioactivity bracket and a preparation method thereof. The bioactivity bracket is obtained through the following steps of firstly, loading bone shape generation proteins on ahollow mesoporous hydroxylapatite microsphere through a grafting manner or an adsorbing manner, so as to obtain a drug bearing microsphere, then mixing the drug bearing microsphere into a bracket matrix solution, then performing printing through a 3D printing technique to prepare a compound bracket, and finally, adsorbing chemotactic growth factors on the surface to obtain the bioactivity bracket;or firstly, blending the drug bearing microsphere and the chemotactic growth factors in the bracket matrix solution, and then performing printing through the 3D printing technique to prepare the bioactivity bracket. Through the adoption of the method disclosed by the invention, the obtained bioactivity bracket can realize time sequence releasing of various growth factors: the chemotactic growth factors are released in an early stage, and bone repair cells are recruited to a damaging region; and bone shape generation proteins are mainly released in the later stage, and bone marrow substrate stem cells which are recruited to the damaging region are induced to be subjected to osteogenic differentiation, so that regeneration and repairing potential of an autologous tissue is sufficiently mobilized, and regeneration and repairing of bone defect are promoted.
Owner:SICHUAN UNIV

LID-PEG-PLGA controlled-release nano microsphere and preparation method thereof

The invention discloses an LID-PEG-PLGA controlled-release nano microsphere and a preparation method thereof. The microsphere is the controlled-release microsphere which contains medicinal lidocaine and a degradable carrier. The degradable carrier contains polylactic-glycolic acid and PEG-2000. The mass percentage of the lidocaine in the controlled-release microsphere is 30 to 35 percent. The preparation method comprises the following steps of: preparing the carrier into matrix solution; dispersing the lidocaine into the matrix solution and preparing the lidocaine into an oil phase; mixing the oil phase and the aqueous solution of polyvinyl alcohol, and performing ultrasonic emulsification on the mixture under a water bath condition to obtain W / O-type protogala; mixing the W / O-type protogala and the aqueous solution of polyvinyl alcohol again, and further emulsifying the mixture into W / O / W-type complex emulsion; volatilizing the emulsion by reducing pressure at the normal temperature to obtain cured lidocaine-carried nano microsphere; and scattering, blending, packaging, freezing, sterilizing and the like. The medicament loading rate of the controlled-release nano microsphere can be up to 15 to 22 percent; the entrapment rate can be up to 68 to 78 percent; and the half-life period can be prolonged to 3 to 4 days. Therefore, the microsphere has relatively good effect of burst in the first day after the microsphere is taken and good effect of slow release in later days.
Owner:ARMY MEDICAL UNIV

Method for testing group of biomarkers

The invention discloses a method for testing a group of biomarkers. The method comprises the following steps: (1) extraction and purification; (2) preparation of standard working solutions, wherein blank samples are processed with the method shown in the step (1), and biomarker series concentration standard working solutions of at least three concentrations are prepared with matrix solutions of the blank samples within the range of 2-100 micrograms per liter; (3) liquid chromatogram-tandem mass spectrometry (LC-MS/MS) determination. A dispersive solid-phase extraction technique is used for the method, a sample pre-processing method capable of avoiding interference of matrixes in the samples easily, conveniently, quickly and effectively is developed, the pre-processing method is combined with the high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and is used for qualitative confirmation and quantitative detection of content of the biomarkers in animal tissue and urine, the recovery rate of the method ranges from 4.2% to 106.2%, the average relative standard deviation (RSD) ranges from 6.4% to 13.0%, the limit of detection is 20.0 microgram per kilogram, the limit of quantitation is 50.0 microgram per kilogram, and the method has the advantages of being easy and convenient to operate, quick, accurate, high in sensitivity and good in repeatability.
Owner:INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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