267 results about "Resolution (mass spectrometry)" patented technology

The invention provides a method for analyzing tobacco flavor components by adopting a multi-dimensional hyphenated chromatographic technique. The method comprises the following steps: 1. extracting the tobacco flavor components; and 2. carrying out liquid chromatography-capillary gas chromatography / mass spectrometry (LC-CGC / MS) online segregation analysis on the tobacco flavor components: A. carrying out normal phase LC segregation on the tobacco flavor components; B. storing the LC effluent components in sample loops of multiposition valves of interfaces respectively; and C. after collection of the LC effluent components is completed, ensuring the fractions stored in the sample loops of the multiposition valves to be driven by carrier gases to respectively enter into a GC / MS to undergo segregation analysis by controlling ten-way valves in the interfaces of the hyphenated instruments. The method has the following prominent advantages: the tobacco flavor components can be segregated in advance through LC, the segregated components are respectively stored in the sample loops by online coupling interfaces and then the components respectively enter into the GC / MS to undergo accurate qualitative and quantitative analysis according to difference of the boiling points. The method not only has better orthogonal segregation property, but also has higher chromatographic peak capacity and better resolution and has simple operation steps.

A data independent acquisition method of mass spectrometry for analysing a sample as it elutes from a chromatography system is disclosed. The method comprises the steps of: ionising the sample to produce precursor ions, selecting a precursor mass range for the sample to be analysed, performing a plurality of MS1 scans and performing at most two sets of MS2 scans. Each of the MS1 scans uses a mass analyser operated at a first, relatively higher resolution, for identification and / or quantitation of the sample in the MS1 domain across the precursor mass range. The set of MS2 scans comprises performing MS2 scans of fragmented mass range segments performed with the mass analyser, operated at a second, relatively lower resolution. In the method, the MS1 scans are interleaved throughout the performing of the set of MS2 scans such that the MS1 scans provide a mass chromatogram of the sample.

The invention relates to spatially resolved mass spectrometric measurement and visualization of the distribution of small molecules in a mass range from approximately 150 to 500 Daltons, for example drugs and their metabolites, in thin sections or other two-dimensional samples, preferably with ionization of the molecules by matrix-assisted laser desorption. The invention includes the steps measuring a daughter ion produced by forced decomposition of the molecular ion instead of the ionized analyte molecule itself, the daughter ion having a much better signal-to-noise ratio. The daughter ions are detected in a relatively simple reflector time-of-flight mass spectrometer instead of using an expensive time-of-flight tandem mass spectrometers for the measurement of the daughter ions. Advantageously, substantially faster and less expensive scanning of the thousands of mass spectra which serve as the basis for visualizing the spatial distribution of the analyte molecule is achieved, while the mass resolution and sensitivity are at least equally good.

The invention discloses a method for screening 207 veterinary drugs and additives in animal food. The method specifically comprises the following steps: preparing a standard working solution; pretreating a to-be-detected sample; establishing a database; detecting a compound; screening the compound; confirming the compound and the like. According to the method, an ultra-high performance liquid chromatography-quadrupole rod / electrostatic field orbitrap high-resolution mass spectrometry technology is utilized to establish a rapid screening and confirmation method for simultaneously detecting 18 types of 207 compounds in animal food within 22 minutes. The method is efficient, the operability is high, good selectivity, precision and sensitivity are realized; convenient and rapid screening can be realized; the false positive rate is low; and the method is especially suitable for screening and confirmation of different kinds of compounds. The invention provides a complete rapid screening andconfirmation detection method for adding various veterinary drugs and additives into animal food, a detection and screening technical means for multiple residues in animal food is added; and the safety guarantee is provided for the national animal husbandry industry and food safety.

Prior work on differential mobility analysis (DMA) combined with mass spectrometry (MS) has shown how to couple the output of a planar DMA with the atmospheric pressure inlet of an atmospheric pressure ionization mass spectrometer (APCI-MS). However, because the ion inlet to APCI-MS instruments is a round orifice, while conventional DMA geometries make use of elongated slits, the coupling of both has attained less resolving power or tolerated a smaller sample flow rate than a DMA alone. The present invention overcomes these limitations with an axial DMA of cylindrical symmetry using more than two electrodes. The configuration is related to that previously proposed by Labowsky and Fernández de la. Mora (2004, 2006), where ions with a critical electrical mobility are brought into the symmetry axis of the DMA. Ions with this critical mobility are now optimally transmitted into the MS, with much higher resolution than possible in planar DMAs. In a preferred embodiment of this DMA facilitating DMA-MS coupling, one DMA electrode intersecting the symmetry axis is relatively planar.

The present invention provides a single set of mass spectrometer capable of selectively performing the following two modes of analyses according to the purpose of analysis: the first mass spectrometry mode in which the analysis can be repeated at short intervals of time; and the second mass spectrometry mode in which the analysis can be performed with high mass resolution and high accuracy. In an embodiment of the present invention, ions ejected from an ion source 1 travel along a straight path B, on which gate electrodes 3 are provided. When a voltage is applied from an MS mode selection controller 7 to the gate electrodes 3, the ions are introduced into a loop orbit A. Located on the loop orbit A is a second ion detector 6, which is a nondestructive type of ion detector. Detection signals of the second ion detector 6 are sent to a data processor 9, which extracts flight time spectrum data from those signals and Fourier-transforms those data to convert the time axis to a frequency axis. From the frequency spectrum thus created, the mass-to-charge ratio of each ion is calculated with high accuracy. When no voltage is applied to the gate electrodes 3, the ions ejected from the ion source 1 travel along the straight path B and arrive at a first ion detector 5. This mode of analysis requires only a short period of time and can achieve a high level of time resolution.

The invention provides an establishing method for a high-resolution mass spectrum database of anti-oxidants and antiseptic, and a detection method for anti-oxidants and antiseptic. The detection method comprises the following steps: respectively preparing single-standard methanol solutions of anti-oxidants and antiseptic and carrying out mass spectrometry with a time-of-flight mass spectrometer in a positive ion full scanning mode and a negative ion full scanning mode so as to establish the high-resolution mass spectrum database; and extracting a to-be-detected sample with alcoholic solutions, carrying out mass spectrometry and detecting the anti-oxidants and antiseptic in the sample according to mass spectral data and the high-resolution mass spectrum database. Compared with the prior art, the invention has the advantages that since high-resolution mass spectrum and target analysis strategies are employed for detection of the anti-oxidants and antiseptic, the method can carry out rapid qualitative detection on a plurality of anti-oxidants and antiseptic at the same time without complex pre-treatment steps; a combination of liquid chromatography and high0resolution mass spectrometry is employed, so the method is high in accuracy and sensitivity; and the method can carry out qualitative determination on screened unknown anti-oxidants and antiseptic, so the scope of analysis and monitoring is broadened.

The invention discloses a method for qualitatively and quantitatively analyzing a key volatile component in cigarette bead blasting. The method comprises the following steps: (1) performing full-scanning analysis on a bead blasting isopropanol solution by a gas chromatography high-resolution time of flight mass spectrometry (GC-TOF / MS) to obtain a full-scanning atlas; (2) determining an unknown material and screening the key component by an area normalization method semiquantitative result; (3) determining the mass spectrometric qualitative and quantitative parameters of the key component by taking n-propyl benzoate as an internal standard substance and by combining gas chromatogram secondary mass spectrometry (GC-MS / MS); and (4) drawing a regression curve by a gas chromatogram secondary mass spectrometry combined multi-monitoring scanning mode, and analyzing the content of the key component accurately and quantitatively. The method is simple in operation, high in sensitivity, high inaccuracy degree, short in analysis time and suitable for performing qualitative and quantitative analysis on various volatile and semi-volatile organic compounds in bead blasting simultaneously.

The invention discloses a method for identifying key toxic substances in pesticide wastewater by taking daphnia magna toxicity as a precursor and belongs to the field of identification of non-target pollutants. The method comprises the following steps: performing enrichment on organic water sample extract; performing a daphnia magna toxicity experiment on an organic water sample extract; performing fractional separation on the organic water sample extract, and separating compounds of different polarities; performing the daphnia magna toxicity experiment on the separated components; and performing qualitative identification and quantitative determination on the compounds in toxic substances. The compounds of different polarities are separated by utilizing a fractional separation technology, the toxic substances are screened to reduce the complexity of a sample, the potential toxic substances are qualitatively identified through a high performance liquid chromatography-time-of-flight mass spectrometry, suspicious toxic substances are quantitatively determined by utilizing a liquid chromatography mass spectrometry, and the defect that key toxic pollutants in a mixing system cannot be obtained by the traditional non-target chemical analysis is overcome. Meanwhile, due to fractional separation and feature object screening, the method is high in resolution ratio, high in accuracy and high in information amount, so that the identification of non-target key toxic substances is relatively convenient and reliable.

The invention discloses a spectroscopy-mass spectrometry combined quantitative analysis device for elements in an unknown sample. The device uses a quantitative analysis structure and a method by using a laser-induced breakdown spectroscopy combining with a second level extraction field time-of-flight mass spectrometry (TOF). The quantitative analysis device with high detection sensitivity, real time, quickness, high accuracy, no contact, capability of simultaneously detecting multiple elements, and no need of pretreating the sample is realized. Due to dual-wavelength laser in the device, photofragments and molecular clusters are re-ionized conveniently, the mass spectrometry signal stability is improved, and a laser plasma emission spectroscopy signal is improved; due to a second level extraction field in the device, the TOF resolution ratio is improved conveniently and the signal is optimized conveniently; due to a spectroscopy collection system in the device, the capability of collecting plasma emission light to couple to optical fiber can be improved, an optical path can be folded, the integration and the miniaturization of an instrument are facilitated, and the commercialization of the instrument is realized conveniently.

The invention discloses a method for simultaneously detecting seven fungal toxins in original auxiliary materials of baijiu. According to the method, an improved QuEChERS method is adopted for extraction, further purification is not needed, high performance liquid chromatography is utilized for achieving separation of the fungal toxins, combined quadrupole-electrostatic field orbitrap mass spectrometry (Q-Orbitrab) is achieved, efficient parent ion selectivity and high-resolution accurate molecular weight (The mass number can be accurate to five digits after the decimal point.) are combined, accurate qualitativeness and quantification of the fungal toxins in baijiu and the original auxiliary materials can be achieved, and quantification is conducted through a matrix external standard method. The fungal toxins can be completely and effectively separated within 9 min, the linear relation of the fungal toxins within separate linear response ranges is good, the correlation coefficient R<2> is larger than 0.999, the lowest limit of quantification is lower than 1.0 microgram / L, the lowest detection limit is lower than 0.1 microgram / L, the recovery rate ranges from 70% to 120%, and the relative standard deviation (RSD) ranges from 0.5% to 4%. Compared with the national standard method, the method has the advantages of being rapid, simple, convenient, economical, efficient, safe and the like and can be suitable for separation and quantitative determination of various fungal toxins in baijiu and original auxiliary material samples.

The invention discloses a method for screening a sweetening agent in milk and dairy products by applying ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry, and belongs to the technical field of food safety inspection. An optimized QuEChERS method is used for preprocessing samples; the ultra-high performance liquid chromatography-quadrupole electrostatic field orbit ion trap mass spectrometry is applied for performing screening, a scanning mode of full-scanning / data-dependency collection and full-scanning / variable data-independent collection and full-scanning / full-ion fragmentation collection is adopted, a positive ion or negative ion mode is selected for scanning, and complete first-order and second-order spectra are obtained; Extract Finder 2.5 software is applied for processing the obtained first-order and second-order spectra, and information of a compound is extracted. Information in a built spectrum library and a tested object screening result are compared, and the composition of the sweetening agent in the milk and dairy products is ensured. An adopted mass spectrometer has the main advantages that the resolution is high, qualitative and quantitative results are accurate, sensitivity is high, and quality precision is high and can be applied to complex matrix analysis. The established screening method has the good application prospect in the field of food.

The invention discloses a detection method for rapidly screening various pesticides and biotoxins in an aquatic product. The detection method comprises the following steps: (1) preparing a standard solution; (2) carrying out QuEChERS extraction and purification of a sample; (3) carrying out concentration; and (4) carrying out analyzing by an ultra-high performance liquid chromatography high-resolution mass spectrometer. QuEChERS is used for pretreating the aquatic product, meanwhile, ultrahigh performance liquid chromatography quadrupole / electrostatic field orbitrap mass spectrometry (UPLC-Q Exactive Orbitrap HRMS is combined to conduct high-throughput measurement on 93 kinds of pesticide and 16 kinds of biotoxin residues in the aquatic product, and the method is sensitive, rapid, simple,accurate, time-saving, stable, high in practicability and capable of meeting the detection requirements of the current market.

The invention discloses a method for determining organic volatile substances in soil by using the gas chromatography-mass spectrometry-headspace. The method for determining the organic volatile substances in the soil by using the gas chromatography-mass spectrometry-headspace comprises the following steps of: step 1, soil collection; step 2, sample preservation and preparation; step 3, headspace analysis; step 4, gas chromatography-mass spectrometry analysis; step 5, standard curve establishment; and step 6, calculation. The method for determining the organic volatile substances in the soil byusing the gas chromatography-mass spectrometry-headspace is characterized in that: the soil is collected into a sample bottle, and the sample bottle is sealed; meanwhile, a soil parallel sample is collected and placed in a sample bottle for sealed storage; then the sample bottles are brought back to a laboratory for analysis; the sample bottles are taken out in the laboratory and returned to theroom temperature; 2g of the sample is weighed and placed into a 22mL headspace bottle; 10mL of matrix correction solution is quickly added to the headspace bottle, and then sealed immediately; chromatographically pure methanol is taken into a sample introduction bottle; and then a volatile standard stock solution is taken into methanol. According to the invention, the organic volatile substances in the sample are not easy to volatilize and lose; the operation is convenient; the resolution is high; the linear range is good; the sensitivity is high; and the interference by the impurity peak is small.

The invention provides a device and a method of rapi`q dly and accurately detecting impurities in high-purity hydrogen for a hydrogen fuel cell. A direct sampling mode is adopted, complex sample pretreatment is omitted, and the cost and the system complexity are greatly reduced; high-purity hydrogen is taken as a carrier gas for quantitative sampling, and a standard gas is taken as reference, so that accurate quantification is realized; the special mass spectrum device for hydrogen detection greatly improves low-end mass number resolution, detection stability and sensitivity, and the system stability is remarkably superior to that of a general chromatographic mass spectrometry method; a special control software system comprises a mass axis automatic calibration software module, a high-purity carrier gas automatic measurement software module, a standard gas quantitative automatic measurement software module, a sample quantitative automatic measurement software module, a sample impuritygas automatic calculation software module, and due to a built-in algorithm, measurement and control automation are realized. The characteristics are beneficial to rapid and accurate determination of the high-purity hydrogen impurities of the hydrogen station of the hydrogen fuel cell.

The invention provides a high-resolution mass spectrometry method for synchronously detecting 152 chemical pollutants in livestock and poultry meat. The method comprises a step of establishing a chemical pollutant standard quality spectrum library, a sample detection step and a screening analysis step. The method covers 152 target compounds such as sulfanilamides, quinolones, quinoxalines, nitroimidazoles, beta-lactams, tetracyclines, macrolides, chloramphenicols, benzimidazoles, nicotinic agonists, beta-receptor agonists, hormones and the like. The method has the advantages that the sample pretreatment method is simple and convenient; the 152 compounds can be screened and confirmed by one-needle sample injection; the detection flux is large; the accuracy is high; and the method is suitable for simultaneous qualitative confirmation detection of various veterinary drug residues in the livestock and poultry meat.

The invention discloses a high performance liquid chromatography-time-of-flight mass spectrometry hyphenated method capable of simultaneously and rapidly screening and identifying alkaloid in a water sample, belonging to the field of analytical chemistry. The method comprises the steps of (A) filtering a filter membrane; (B) extracting a solid phase columella; (C) eluting a solvent; (D) concentrating and metering the volume; and (E) detecting on a machine, wherein the sample is screened and identified by a high performance liquid chromatography--time-of-flight mass spectrometry. The high performance liquid chromatography-time-of-flight mass spectrometry is adopted for testing and is cooperated with the analytical method of a peak view comprehensive assessment grading software to screen and survey the water sample in large scale, the water sample is scanned under a first mass spectrum mode and a second mass spectrum mode, the resolution ratio is high, the accuracy is high, the scanning and a compound substance library are matched through a second mass spectrum spectrogram peak shape, the quantitative advantage is obvious, the result of purity score is displayed concisely and intuitively, and the result is more accurate and reliable.

The invention relates to a method for screening a feature marker for identifying a honey producing place. According to the method, a simple sample pre-treatment process is performed; then, a great amount of high-resolution mass spectrum data is obtained by an ultra-high performance liquid chromatography-quadrupole / electrostatic field orbital hydrazine high-resolution mass spectrometry; deviation and variation interference in experiment data are eliminated through background removal, chromatographic peak extraction, normalization, data conversion and data zooming; then, through main ingredient analysis, t-inspection, Volcano plots and variable importance projection drawing, the real feature markers capable of distinguishing the same honey source honey from different producing places can be discovered and verified. After the feature marker is used for identifying the honey producing place, identification results are objective and precise; the method can be used for the variety source tracing identification of honey of the same honey source from different producing places, and can also be used for the variety source tracing and producing place tracing identification of other farm products.

An analysis method for dynamically tracing and monitoring nicotine and nicotine metabolites in an animal is characterized in that the animal is exposed after pyridine ring 2H labeled nicotine ([2H4]-(+ / -)-Nicotine) and unlabeled nicotine are mixed in equal proportion, a micro-dialysis system is utilized to respectively collect blood and brain dialysate samples in a micro-centrifuge tube, the samples are directly put on an ultra high performance liquid chromatography-orbit trap to conduct high-resolution mass spectrometry detection on the nicotine metabolites and pyridine ring 2H labelers of the nicotine metabolites in the micro-dialysis samples after an analyzing interior label is added for short-term low-speed centrifugation, the nicotine metabolites are identified according to test results, and a corresponding time-concentration curve is drawn for dynamic monitoring and analysis. The analysis method has the advantages that a variety of nicotine metabolites in animals can be accurately traced and identified, a sample pretreatment step is greatly simplified, accurate analysis and synchronous comparison of nicotine metabolite changes in different systems in animals are achieved, and a novel method for researching the nicotine metabolites in animals is created.

The invention relates to a method for rapidly analyzing lipid components in bee pollen. The method comprises the following steps: using an ultra performance liquid chromatography and quadrupole-static electric field orbitrap high resolution mass spectrum coupling technique (UPLC-Q-Exactive Orbitrap MS), and using positive and negative ion monitoring mode, full-scanning and the function of automatically triggering a tandem mass spectrometry scanning to accurately realize precise quantification of lipid components in bee pollen from different plants, and providing the basis for the origin place of bee pollen, authenticity judgment, doping counterfeiting and functional nutriology research of bee pollen. The technology is high in resolution ratio and accurate in positioning, overcomes the problems of low accuracy and reliability in ingredient identification in the prior art, and is a novel efficient technology for lipid component analysis.

The invention discloses a Xinjiang artemisia plant metabonomics analysis method. The method comprises the following steps: 1) taking dry powder of different tissue organs of Xinjiang artemisia, respectively performing steps of extraction with an organic solvent, ultrasonic wave, centrifugation, and condensation to obtain a concentrate, then performing redissolving, ultrasonic wave, centrifugationand filtering on the concentrate, and taking supernatants of metabolome of the different tissue organs; 2) employing a liquid chromatogram-tandem mass spectrometry on the supernatants of metabolome ofthe different tissue organs, and processing the obtained data; employing multivariable statistics analysis to obtain differential variables, according to a high-resolution mass-to-charge ratio and tandem mass spectrum data of the differential metabolite, and combining a metabolite database Metlin retrieval contrast analysis, obtaining the difference of the plant metabolome of the different tissueorgans of the Xinjiang artemisia. The method has the advantages that pre-treatment operation on a sample is simple, the stability of an analysis method is good, sensitivity is high, a metabolite covering range is wide, and the metabolome information of the plant can be efficiently and comprehensively obtained.

The invention relates to a high performance liquid chromatography-time-of-flight mass spectrometry method for identifying a degradation product in a compound cold treatment medicine based on a heart-cutting technology. The method comprises the following steps: separating a sample by using a mass spectrum incompatible mobile phase in a first-dimensional chromatograph; acquiring ultraviolet data through a one-dimensional detector; determining a target peak, and carrying out heart-cutting to make the target peak exist in a sample loop; carrying out back flushing through valve switching by using a two-dimensional system using a mass spectrum compatible mobile phase to make the target peak exist in the two-dimensional system and enter a two-dimensional ultraviolet and mass spectrum detector in order to complete impurity analysis and identification. The method has the advantages of no change of original separation conditions, high specificity, accuracy and resolution in impurity detection, and improvement of the reliability of nonvolatile buffer salt separation system impurity mass spectrometry and identification.

The invention relates to a method for simultaneous chiral resolution and determination of nicotine and nornicotine in electronic cigarette liquid. The method comprises the following steps: 1) mixing the electronic cigarette liquid with isopropanol and successively carrying out ultrasonic extraction and filtering so as to obtain a to-be-detected solution; and 2) analyzing the to-be-detected solution obtained in the step 1) by using ultra-performance convergence chromatography-tandem mass spectrometry. The method for simultaneous chiral resolution and determination of nicotine and nornicotine in the electronic cigarette liquid is simple in pre-treatment, rapid in determination and high in sensitivity and is a good method for determination of chiral substances in nicotiana alkaloids; and the method does not use any chiral shift reagent, can realizes simultaneous resolution of chiral isomers of the two alkaloids, i.e., nicotine and nornicotine, in the electronic cigarette liquid, and is high in analysis efficiency and friendly to environment.

The invention discloses a new Gelsmium elegans Benth. alkaloid compound as well as a preparation method and application thereof. In the preparation method, a target compound is separated from Gelsmium elegans Benth. by using a pH-zone-refining countercurrent chromatography and taking a total Gelsmium elegans Benth. alkaloid crude extract as a raw material and a high-speed counter-current chromatographic instrument as separation equipment; and the method has the advantages of large preparation amount, less sample loss and strong controllability, and is convenient and fast and is suitable for industrial production. By the determination of high-resolution mass spectrometry, ultraviolet spectrum, infrared spectrum, <1>H, <13>C-NMR (nuclear magnetic resonance) spectrum, <1>H-<1>HCOSY (correlation spectrometry), HSQC (heteronuclear single quantum coherence) spectrum and HMBC (heteronuclear multiple-bond correlation) spectrum, the new Gelsmium elegans Benth. alkaloid compound has the structure of a formula (I). A pharmacological test shows that the compound in the invention has dose dependence, powerful analgesic effect, less tolerance, addiction and side effect and treatment index far higher than that of total Gelsmium elegans Benth. alkaloid, can be used for preparing a novel powerful low-toxicity medicament or lead compound used for treating pain, and has definite industrial prospect.

The present invention discloses a novel antimicrobial peptide and an application thereof in citrus preservation. A linear tetrapeptide which has a strong inhibition effect on rotten fungi after citrusharvesting is separated out from fermentation liquid of penicillum oxalicum SG-4 by steps of ethanol precipitation, concentration and freeze-drying, high performance liquid chromatography, etc. Aftermagnetic resonance imaging, high-resolution mass spectrometry and other spectrum analysis, the substance is found to be a propylene glycol monomethyl ether connected linear 4-peptide compound Thr-Thr-Val-Ser (propanediol tetrapeptide, PTP for short), parts of peptide bonds and alpha-amino groups show methylation, and structures and functions of the type of the compound are not reported internationally. Experiments show that the PTP has the strong inhibition effect on rotten fungi penicillium italicum after the citrus harvesting, antimicrobial functions also exist when PTP is at a low concentration of 1 [mu]g / mL, and the effect of the PTP is significantly better than commonly used citrus preservatives of bellkute, a prochloraz and imazalil compound, carbendazim, etc. in the market. The antibiotic is a potential citrus preservative and can be used on a large scale in industrial and agricultural production.

Disclosed is a method for detecting disulfide bond breakage of protein. The method for detecting disulfide bond breakage of the protein comprises utilizing iodoacetic acid to react with free sulfydryl, removing unreacted iodoacetic acid through filtration, breaking the disulfide bonds inside the protein through dithiothreitol, utilizing ammonium iodoacetate to react with newly-produced sulfydryl, utilizing trypsin to hydrolyze the protein into polypeptide, detecting the mass to charge ratio information of the polypeptide through high-resolution mass spectrometry and comparing the mass to charge ratio information with the disulfide bond information of natural protein to determine whether the disulfide bonds break. The method for detecting disulfide bond breakage of the protein can be effectively used for determining whether the sulfydryl exists in a free or disulfide mode; the mass difference caused by reactions between the iodoacetic acid as well as the ammonium iodoacetate and the sulfydryl can be within 1Da, so that the difference between the two reactions can be determined very accurately through the high-resolution mass spectrometry; accurate judgment on whether the disulfide bonds of the protein break can lay the foundation for relevant theoretical researches and provide theoretical foundation for modification of specific proteins and actual production.

The embodiment of the application provides an accelerator mass spectrometry system and relates to the technical field of AMS. The accelerator mass spectrometry system comprises an ECR strong current positive ion source subsystem, an injector subsystem, a strong current accelerator subsystem, a high-energy analysis subsystem and a high-resolution detector subsystem which are successively connected.The ECR strong current positive ion source subsystem is configured to generate strong current positive ions in multiple charge states. The strong current accelerator subsystem is configured to directly accelerate the strong current positive ions. The accelerator mass spectrometry system has the advantages of strong beam current, high total efficiency, and good background lowering capability, andcan greatly improve the abundance sensitivity of the spectrometry.

The invention provides an analysis method of lipid components in Fuzhuan tea. The method is used for carrying out lipidomic analysis on a tea sample and carrying out structural identification on lipidsubstances. According to the invention, after tea powder of Fuzhuan tea is processed, a total ion flow diagram of lipid substance components in a Fuzhuan tea sample is obtained by adopting a tea lipidomic analysis method of ultra-high-phase liquid chromatography-quadrupole and orbital hydrazine high-resolution mass spectrometry combined UHPLC-Q Exactive MS, and original data of the total ion flowdiagram of the Fuzhuan tea sample is introduced into a lipid qualitative software to carry out structural identification on the lipid substances in the Fuzhuan tea, so that a theoretical basis is provided for regulation and control of the quality of the Fuzhuan tea.

The invention discloses a method for detecting the content of NDMA in a tidine drug. The method adopting liquid chromatography-mass spectrometry detection comprises the following steps: (1), separating a tidine drug sample by using liquid chromatography that adopts a hydrophilic interaction chromatographic column, and has no acid added into a pre-column mobile phase; and (2), detecting the NDMA content of the sample subjected to liquid chromatography separation in combination with mass spectrometry. According to the invention, a hydrophilic interaction chromatographic analysis method is adopted to separate the tidine component and the NDMA; acid is not added into the pre-column mobile phase, so that the tidine and the NDMA are kept in a non-ionic state to be separated, the separation timedifference between the tidine and the NDMA is 12-13 minutes; and thus the interference of tidine components on the NDMA can be completely removed, and the quantitative result is accurate. According tothe method, electrospray ionization is combined with tandem mass spectrometry, ESI is low-temperature mass spectrometry, the mass spectrometry part is non-high in resolution, and the manufacturing cost is relatively low, so that the application and popularization performance is higher, and the conversion and application prospect is good.