2 results about "Dammarane" patented technology

The present application relates to the field of biotechnology, in particular to a method for heterologous synthesis of dammarane-type ginsenosides in the endosperm of crop seeds, a vector, a plant and application. Through modular design, a core gene module driven by an endosperm-specific promoter is assembled in the T-DNA region of the vector, and a downstream glycosylation or hydroxylation-glycosylation modification module is configured to realize the directional synthesis of PPD-type and PPT-type ginsenosides, respectively. The transgenic crops obtained by using the vector can specifically accumulate target ginsenosides in the endosperm. The obtained ginseng rice shows clear anti-tumor activity in in vitro tumor cell experiments and tumor-bearing mouse models. The present application provides a new strategy for low-cost production of high-value natural products, and opens up a new direction for the application of functional crops in the field of medicine.

The invention discloses a pseudo-ginseng-derived glycosyl transferase and an application thereof in preparation of pseudo-ginseng or ginsenoside. Specifically, genes capable of catalyzing xylose glycosylation of dammarane type ginsenoside C3-Glc-(2, 1) Glc, xylose glycosylation of C20-Glc and glycosylation modification of arabinopyranose are identified in a pseudo-ginseng genome, and the genes are named as UXT3, UXT4, UXT5, UXT6, UXT8, UXT9, UXT10 and UXT12. Products such as ginsenoside Rb3, ginsenoside Rb2, ginsenoside Rd2, ginsenoside Y, ginsenoside F3, ginsenoside Fa, ginsenoside R4, ginsenoside Fc, ginsenoside Fd, ginsenoside Fz, ginsenoside FP1, ginsenoside FP2, ginsenoside Ft1, ginsenoside R1-Xyl, ginsenoside XIII, ginsenoside R7, ginsenoside LM1, ginsenoside L17, ginsenoside E and the like are obtained. And a new gene is provided for artificially synthesizing a ginseng / notoginsenoside monomer.