11 results about "Dammarane" patented technology

The invention discloses a gynostemma pentaphyllum transcription factor GpMYC65 and application thereof. The nucleotide sequence of the gynostemma pentaphyllum transcription factor GpMYC65 is as shown in SEQ ID NO. 1. The invention also discloses a preparation method of the gynostemma pentaphyllum transcription factor GpMYC65. The amino acid sequence of the protein encoded by the gynostemma pentaphylla transcription factor GpMYC65 is as shown in SEQ ID NO. 2. The heavy recombinant vector contains a gynostemma pentaphylla transcription factor GpMYC65. The invention relates to application of a Gynostemma pentaphyllum transcription factor GpMYC65 in regulating and controlling the content of dammarane type triterpenoid saponin in Gynostemma pentaphyllum. The application comprises the following steps: step 1, constructing an overexpression vector containing a transcription factor GpMYC65 encoding gene; 2, transforming the overexpression vector into gynostemma pentaphyllum leaves by using an agrobacterium-mediated method to obtain genetic transformation hairy roots, and screening and identifying positive hairy roots of an overexpression transcription factor GpMYC65; and 3, carrying out liquid culture on the screened positive hairy roots to obtain transgenic hairy roots of which the dammarane type triterpenoid saponin content is higher than that of the wild hairy roots. The content of dammarane type triterpenoid saponin can be remarkably increased by overexpressing the transcription factor GpMYC65 in the fiveleaf gynostemma herb hairy roots.

The invention belongs to the technical field of medicinal chemistry, and particularly relates to a dammarane sapogenin-isatin derivative and application thereof in anti-tumor treatment. The dammarane sapogenin-isatin conjugate is constructed by taking dammarane sapogenin as a mother nucleus, deriving the dammarane sapogenin and chloroacetic acid and then introducing an indolone structural unit, and R1, R2 and R3 are as described in the claims and the specification. According to the invention, isatin fragments and ginsenoside are coupled for the first time, the anti-tumor activity of the compounds is systematically evaluated, and the action mechanism research of the compounds in colorectal cancer and other tumors is further developed. Results show that the obtained compound has relatively good anti-tumor efficacy, tumor cell selectivity, relatively low toxicity and relatively high bioavailability, and shows good patent medicine potential and wide market prospects.

The invention belongs to the field of traditional Chinese medicine chemistry and natural product separation, and particularly relates to extraction and separation of a novel triterpenoid compound 25-Etherxy-dammar-20 (22) Z-ene-3beta, 6alpha, 12beta-triol and application of the novel triterpenoid compound 25-Etherxy-dammar-20 (22) Z-ene-3beta, 6alpha, 12beta-triol in myocardial protection drugs. The compound is named as 25-Etherxy-dammar-20 (22) Z-ene-3beta, 6alpha, 12beta-triol, and the structural formula of the compound is shown in the description. The invention provides an extraction and separation method of the compound. The extraction and separation method mainly comprises the following steps: sequentially carrying out solvent extraction, macroporous adsorption resin enrichment, silica gel column chromatography, ODS medium-pressure column chromatography, high performance liquid chromatography separation and purification and the like on ginseng and other traditional Chinese medicine processed products and hydrolysates. The structure of the compound is determined through comprehensive analysis of a high-resolution mass spectrum (HRMS), one-dimensional nuclear magnetic resonance (1H NMR, 13C NMR) and a two-dimensional nuclear magnetic resonance spectrum (such as HSQC, HMBC, ROESY and the like), and the compound is a newly discovered dammarane type triterpene compound. Experiments show that the compound and the composition thereof have a remarkable protection effect on myocardial cell hypoxia / reoxygenation reperfusion injury, and can be used for preparing drugs for preventing or treating myocardial ischemia reperfusion injury and other related diseases.

The invention discloses a dammarane type triterpenoid compound or a pharmaceutically acceptable salt thereof. The dammarane type triterpenoid compound has a structural formula as shown in a formula I which is described in the specification. The triterpenoids which are extracted and separated from a gynostemma pentaphyllum medicinal material and have a new chemical structure have an obvious effect of promoting ulcer healing, the application range of gynostemma pentaphyllum can be expanded by developing the triterpenoids into medicines for treating diseases and symptoms related to ulcer, and the utilization value of gynostemma pentaphyllum plant resources is improved. # imgabs0 #

The invention discloses a dammarane type triterpenoid compound or a pharmaceutically acceptable salt thereof. The dammarane type triterpenoid compound has a structural formula as shown in a formula I which is described in the specification. The triterpenoids which are extracted and separated from a gynostemma pentaphyllum medicinal material and have a new chemical structure have an obvious anti-infection effect, the application range of gynostemma pentaphyllum can be expanded by developing the triterpenoids into medicines for treating infection-related diseases and symptoms, and the utilization value of gynostemma pentaphyllum plant resources is improved. # imgabs0 #

The present application relates to the field of biotechnology, in particular to a method for heterologous synthesis of dammarane-type ginsenosides in the endosperm of crop seeds, a vector, a plant and application. Through modular design, a core gene module driven by an endosperm-specific promoter is assembled in the T-DNA region of the vector, and a downstream glycosylation or hydroxylation-glycosylation modification module is configured to realize the directional synthesis of PPD-type and PPT-type ginsenosides, respectively. The transgenic crops obtained by using the vector can specifically accumulate target ginsenosides in the endosperm. The obtained ginseng rice shows clear anti-tumor activity in in vitro tumor cell experiments and tumor-bearing mouse models. The present application provides a new strategy for low-cost production of high-value natural products, and opens up a new direction for the application of functional crops in the field of medicine.

The invention discloses a dammarane type triterpenoid compound or a pharmaceutically acceptable salt thereof. The dammarane type triterpenoid compound has a structural formula as shown in a formula I which is described in the specification. The triterpenoids which are extracted and separated from a gynostemma pentaphylla medicinal material and have a new chemical structure have an obvious antipyretic effect, the application range of gynostemma pentaphylla can be expanded by developing the triterpenoids into medicines for treating diseases and symptoms related to body temperature rise, and the utilization value of gynostemma pentaphylla plant resources is improved. # imgabs0 #

The invention discloses a dammarane type tetracyclic triterpenoid saponin composition and application thereof, and belongs to the technical field of biological medicine. According to the invention, three saponins (notoginsenoside Ft1, 20 (R)-ginsenoside Rh2 and 20 (R)-ginsenoside Rg3) are combined to prepare the composition, and the composition can effectively inhibit the increase of triglyceride (TG), total cholesterol (TC), malondialdehyde (MDA), aspartate transaminase (AST) and alanine transaminase (ALT) of mouse livers in a high-dose (40mg / kg) group and a medium-dose (20mg / kg) group; the synthesis of glutathione (GSH) is effectively promoted, and the reduction of mouse body weight and the weight gain of liver are restrained; the high-dose composition effectively inhibits the generation of liver fat particles; the traditional Chinese medicine composition has a good treatment effect on alcoholic fatty liver.

The invention belongs to the field of application of anti-adenocarcinoma drugs, and relates to medical application of a neodammarane derivative, in particular to application of the neodammarane derivative in preparation of anti-pancreatic cancer drugs. According to the application disclosed by the invention, by investigating the inhibition effect of a series of new dammarane type derivatives such as PD, 25M-PPD and 25H-PPD or pharmaceutically acceptable salts thereof on a plurality of pancreatic cancer cells, the series of compounds disclosed by the invention are found to be capable of remarkably inhibiting the growth and proliferation of the plurality of pancreatic cancer cells (Panc-1, HPAC, Capan-2 and PaTu 8988t) and inducing the differentiation and apoptosis of the pancreatic cancer cells (Panc-1, HPAC, Capan-2 and PaTu 8988t). Part of the compounds also have inhibition effects on prostatic cancer and breast cancer. Therefore, the neodammarane derivative or the pharmaceutically acceptable salt thereof can be used for preparing anti-adenocarcinoma drugs.

The invention provides a dammarane type triterpenoid saponin enzymolysis derivative as well as a preparation method and application thereof. A series of dammarane type triterpenoid saponin enzymolysis derivatives are prepared by selectively hydrolyzing glycosyl through glycosidase; the preparation method disclosed by the invention can be used as a powerful means for preparing triterpenoid saponin series derivatives. In addition, through enzymolysis of the dammarane type triterpenoid saponin glycosyl, the AMPK agonistic activity and the biological activity of inhibiting hepatic stellate cells are increased, and the dammarane type triterpenoid saponin glycosyl can be used for preparing the medicine for preventing or treating fibrosis diseases represented by hepatic fibrosis and liver cirrhosis.

The invention discloses a pseudo-ginseng-derived glycosyl transferase and an application thereof in preparation of pseudo-ginseng or ginsenoside. Specifically, genes capable of catalyzing xylose glycosylation of dammarane type ginsenoside C3-Glc-(2, 1) Glc, xylose glycosylation of C20-Glc and glycosylation modification of arabinopyranose are identified in a pseudo-ginseng genome, and the genes are named as UXT3, UXT4, UXT5, UXT6, UXT8, UXT9, UXT10 and UXT12. Products such as ginsenoside Rb3, ginsenoside Rb2, ginsenoside Rd2, ginsenoside Y, ginsenoside F3, ginsenoside Fa, ginsenoside R4, ginsenoside Fc, ginsenoside Fd, ginsenoside Fz, ginsenoside FP1, ginsenoside FP2, ginsenoside Ft1, ginsenoside R1-Xyl, ginsenoside XIII, ginsenoside R7, ginsenoside LM1, ginsenoside L17, ginsenoside E and the like are obtained. And a new gene is provided for artificially synthesizing a ginseng / notoginsenoside monomer.