5 results about "Dammarane" patented technology

The invention belongs to the technical field of medicinal chemistry, and particularly relates to a dammarane sapogenin-isatin derivative and application thereof in anti-tumor treatment. The dammarane sapogenin-isatin conjugate is constructed by taking dammarane sapogenin as a mother nucleus, deriving the dammarane sapogenin and chloroacetic acid and then introducing an indolone structural unit, and R1, R2 and R3 are as described in the claims and the specification. According to the invention, isatin fragments and ginsenoside are coupled for the first time, the anti-tumor activity of the compounds is systematically evaluated, and the action mechanism research of the compounds in colorectal cancer and other tumors is further developed. Results show that the obtained compound has relatively good anti-tumor efficacy, tumor cell selectivity, relatively low toxicity and relatively high bioavailability, and shows good patent medicine potential and wide market prospects.

The invention belongs to the field of traditional Chinese medicine chemistry and natural product separation, and particularly relates to extraction and separation of a novel triterpenoid compound 25-Etherxy-dammar-20 (22) Z-ene-3beta, 6alpha, 12beta-triol and application of the novel triterpenoid compound 25-Etherxy-dammar-20 (22) Z-ene-3beta, 6alpha, 12beta-triol in myocardial protection drugs. The compound is named as 25-Etherxy-dammar-20 (22) Z-ene-3beta, 6alpha, 12beta-triol, and the structural formula of the compound is shown in the description. The invention provides an extraction and separation method of the compound. The extraction and separation method mainly comprises the following steps: sequentially carrying out solvent extraction, macroporous adsorption resin enrichment, silica gel column chromatography, ODS medium-pressure column chromatography, high performance liquid chromatography separation and purification and the like on ginseng and other traditional Chinese medicine processed products and hydrolysates. The structure of the compound is determined through comprehensive analysis of a high-resolution mass spectrum (HRMS), one-dimensional nuclear magnetic resonance (1H NMR, 13C NMR) and a two-dimensional nuclear magnetic resonance spectrum (such as HSQC, HMBC, ROESY and the like), and the compound is a newly discovered dammarane type triterpene compound. Experiments show that the compound and the composition thereof have a remarkable protection effect on myocardial cell hypoxia / reoxygenation reperfusion injury, and can be used for preparing drugs for preventing or treating myocardial ischemia reperfusion injury and other related diseases.

The present application relates to the field of biotechnology, in particular to a method for heterologous synthesis of dammarane-type ginsenosides in the endosperm of crop seeds, a vector, a plant and application. Through modular design, a core gene module driven by an endosperm-specific promoter is assembled in the T-DNA region of the vector, and a downstream glycosylation or hydroxylation-glycosylation modification module is configured to realize the directional synthesis of PPD-type and PPT-type ginsenosides, respectively. The transgenic crops obtained by using the vector can specifically accumulate target ginsenosides in the endosperm. The obtained ginseng rice shows clear anti-tumor activity in in vitro tumor cell experiments and tumor-bearing mouse models. The present application provides a new strategy for low-cost production of high-value natural products, and opens up a new direction for the application of functional crops in the field of medicine.

The invention belongs to the field of application of anti-adenocarcinoma drugs, and relates to medical application of a neodammarane derivative, in particular to application of the neodammarane derivative in preparation of anti-pancreatic cancer drugs. According to the application disclosed by the invention, by investigating the inhibition effect of a series of new dammarane type derivatives such as PD, 25M-PPD and 25H-PPD or pharmaceutically acceptable salts thereof on a plurality of pancreatic cancer cells, the series of compounds disclosed by the invention are found to be capable of remarkably inhibiting the growth and proliferation of the plurality of pancreatic cancer cells (Panc-1, HPAC, Capan-2 and PaTu 8988t) and inducing the differentiation and apoptosis of the pancreatic cancer cells (Panc-1, HPAC, Capan-2 and PaTu 8988t). Part of the compounds also have inhibition effects on prostatic cancer and breast cancer. Therefore, the neodammarane derivative or the pharmaceutically acceptable salt thereof can be used for preparing anti-adenocarcinoma drugs.

The invention discloses a pseudo-ginseng-derived glycosyl transferase and an application thereof in preparation of pseudo-ginseng or ginsenoside. Specifically, genes capable of catalyzing xylose glycosylation of dammarane type ginsenoside C3-Glc-(2, 1) Glc, xylose glycosylation of C20-Glc and glycosylation modification of arabinopyranose are identified in a pseudo-ginseng genome, and the genes are named as UXT3, UXT4, UXT5, UXT6, UXT8, UXT9, UXT10 and UXT12. Products such as ginsenoside Rb3, ginsenoside Rb2, ginsenoside Rd2, ginsenoside Y, ginsenoside F3, ginsenoside Fa, ginsenoside R4, ginsenoside Fc, ginsenoside Fd, ginsenoside Fz, ginsenoside FP1, ginsenoside FP2, ginsenoside Ft1, ginsenoside R1-Xyl, ginsenoside XIII, ginsenoside R7, ginsenoside LM1, ginsenoside L17, ginsenoside E and the like are obtained. And a new gene is provided for artificially synthesizing a ginseng / notoginsenoside monomer.