406 results about "Glycosyl" patented technology

According to the invention, a glycosyl transferase mutant derived from Bacillus licheniformis ZSP01 is used as a research object, AlphaFold 2 is used for constructing a three-dimensional structure of a protein, then technologies such as molecular docking and molecular dynamics simulation are used for analyzing amino acids far away from a catalytic center in glycosyl transferase, site-directed mutagenesis is carried out on the amino acids, and the glycosyl transferase is obtained. The activity of the mutant for catalytically synthesizing the icariside D2 glycosyltransferase is improved.

The invention belongs to the field of food, and particularly relates to application of a nutritional composition for assisting in improving memory ability. The invention provides application of a nutritional composition in preparation of food for assisting in improving memory ability of offspring. The nutritional composition comprises the following necessary components: active folic acid substances and fucosyllactose, moreover, in the nutritional composition, the mass ratio of the active folic acid substance to the fucosyllactose is (0.0001-0.0015): (0.5-10), the food is ingested by a mother to play the role of assisting in improving the memory ability of the offspring, the mother is in a gestation period and / or a lactation period, and the maternal is in the gestation period and / or the lactation period. The filial generation comprises a fetal-stage filial generation and / or an infant-stage filial generation and an optional child-stage filial generation. A large number of studies find that when active folic acid substances and fucosyllactose which are combined according to a certain proportion are supplemented to a mother at the beginning of the gestation period, the memory ability of offspring can be remarkably improved in an auxiliary mode.

According to the present invention, glycosyl transferase UGTBL1 derived from Bacillus licheniformis ZSP01 is adopted as a research object, AlphaFold 2 is adopted to carry out modeling, molecular docking, molecular dynamics simulation and other technologies, amino acids in an active pocket region of glycosyl transferase are analyzed, site-directed mutagenesis is performed on the amino acids to obtain a series of glycosyl transferase mutants, and salidroside can be highly selectively catalyzed and synthesized.

The invention discloses an efficient extraction method of large-molecular-weight astragalus polysaccharide, which belongs to the technical field of plant extraction, and comprises the following steps: cleaning astragalus, filtering, and crushing; the preparation method comprises the following steps: mixing a beta-1, 3-glucanase aqueous solution and trehalose ethylene diamine, and stirring for 10-30 minutes to obtain an enzyme protection system; soaking the radix astragali powder in a citric acid solution, adding an enzyme protection system, applying a bipolar pulsed electric field, and reacting at 40-60 DEG C for 30-40 minutes; adjusting the concentration of the ethanol to 40-50%, standing, centrifuging and collecting crude astragalus polysaccharide; purifying, decolorizing, concentrating, freeze-drying and crushing the crude astragalus polysaccharide to obtain refined astragalus polysaccharide; the mass ratio of the trehalose ethylene diamine to the beta-1, 3-glucanase is (15: 1) to (30: 1). The enzyme protection system maintains the activity of the enzyme, the enzyme and the pulsed electric field act synergistically, the extraction time is shortened, and the extraction rate is increased while the astragalus polysaccharide with large molecular weight is obtained.

The invention relates to the field of microbial metabolism engineering, and discloses a method for increasing the yield of 2 '-fucosyllactose (2'-FL) through the synergistic effect of various alpha-1, 2-fucosyltransferases. According to the method, two or more alpha-1, 2-fucosyltransferases with different sources are co-expressed in microbial cells, and the complementary enzymatic characteristics of the alpha-1, 2-fucosyltransferases are utilized, so that the synthesis efficiency of the 2 '-FL is remarkably improved, and the yield of the 2'-FL is effectively improved. The microbial cell is escherichia coli C43 (DE3) (E. coli C43 lacZ waaF with lacZ and waaF genes knocked out, the serial number is SL), a pETDuet-1 plasmid is constructed, and genes manB, manC, gmd, wcaG and zwf are overexpressed on a pETDuet-1 vector, so that the microbial cell can be used for preparing the microbial cell. In a 5L fermentation tank, after the final strain SL07 is used for final fermentation for 68 hours, the yield of 2 '-FL reaches 148g / L, and the production intensity reaches 2.18 g / L / h. The embodiment shows that the engineering strain for co-expressing the alpha-1, 2-fucosyltransferase from different sources can obviously improve the yield of the 2 '-FL.

The use of a nutritional composition in the preparation of a food product for assisting in improving the memory ability in offspring, wherein the nutritional composition contains the following essential components: an active folic acid substance and fucosyllactose; and in the nutritional composition, the mass ratio of the active folic acid substance to the fucosyllactose is (0.0001-0.0015):(0.5-10). The food product exerts the effect of assisting in improving the memory ability in offspring, wherein the food product is ingested by a mother, the mother is preganant and / or lactating, and the offspring comprises fetal offspring and / or infant offspring and optionally childhood offspring.

The invention belongs to the field of functional substance research, and particularly relates to a nutritional composition for promoting absorption of fatty acid and mineral substances and application. The nutritional composition provided by the invention comprises necessary active ingredients shown as the following (I) and (II): (I) 1, 3-dioleic acid-2-palmitic acid triglyceride; (II) a milk fat globule membrane, and / or, 2 '-fucosyllactose; moreover, in the nutritional composition, the mass ratio of the necessary active component shown in (I) to the necessary active component shown in (II) is (5-10): (0.5-10). The combination of OPO and MFGM and / or 2 '-FL provided by the invention can be beneficial to promoting the absorption of the body to fatty acids and mineral substances, and especially can be beneficial to promoting the transmembrane transport capability of intestinal cells of the body to fatty acids and mineral substances, so that the absorption efficiency is improved.

The invention belongs to the technical field of biology, and particularly relates to bifidobacterium longum subsp.infantis FMBL B250020GXY for metabolizing breast milk oligosaccharide and application of the bifidobacterium longum subsp.infantis FMBL B250020GXY, the bifidobacterium longum subsp.infantis FMBL B250020GXY is preserved in China Center for Type Culture Collection on September 1, 2025, and the preservation number is CCTCC M 20251931; three kinds of breast milk oligosaccharides, namely 2 '-fucosyllactose (2'-FL), lactose-N-neotetraose (LNnT) and lactose-N-tetraose (LNT), can be metabolized; plant source carbohydrates such as fructo-oligosaccharide and synanthrin can be metabolized; the compound has a relatively strong capability of removing DPPH and ABTS free radicals; a good inhibition effect is achieved on common pathogenic bacteria; good tolerance to acid and cholate is achieved; the strain is sensitive to gentamicin, streptomycin, tetracycline, chloramphenicol and vancomycin; the compound can be used for preparing antioxidant products, medicines for inhibiting pathogenic bacteria, infant foods and fermented foods, and has a wide application prospect.

The present invention relates to a combination of galacto-oligosaccharides (GOS) and a mixture of human milk oligosaccharides (HMOs), or nutritional compositions comprising the combination. The combination or compositions are for use in treating and / or preventing stress and / or a mood disorder in an infant, young child and / or child. The mixture of HMOs consists or consists essentially of 2′-fucosyllactose (2′FL) and difucosyllactose (DFL) or of lacto-N-tetraose (LNT) and 6′ sialyllactose (6′SL). The present invention also relates to the use of a combination of galacto-oligosaccharides (GOS) and a mixture of human milk oligosaccharides (HMOs), or compositions comprising the combination, for treating and / or preventing stress and / or a mood disorder in an infant, young child and / or child, wherein the mixture of HMOs consists or consists essentially of 2′-fucosyllactose (2′FL) and difucosyllactose (DFL) or of lacto-N-tetraose (LNT) and 6′ sialyllactose (6′SL).

The present invention provides a composition for use in the prevention and / or treatment of a viral infection in an individual wherein the composition comprises Bifidobacterium longum subsp. Infantis and a mixture of HMO consisting of 2 '-fucosyllactose (2FL), difucosyllactose (DFL), lactose-N-tetraose (LNT), 6'-sialyllactose (6SL), 3 '-sialyllactose (3SL), and 3-fucosyllactose (3FL). The present invention also provides compositions for use in preventing and / or reducing the risk of allergen sensitization, preventing and / or reducing the risk of developing respiratory disorders in an individual, and / or preventing and / or reducing the risk of developing asthma in an individual.

The invention belongs to the technical field of biology, and particularly relates to a construction method and application of a tobacco plant for expressing rare ginsenoside. According to the method, dammarendiol synthase DDS, cytochrome P450 CYP716A47 and glycosyl transferase UGTPg1 / UGTPg45 are added into tobacco, heterologous synthesis of rare ginsenosides CK and Rh2 is achieved, dependence on traditional ginseng cultivation extraction is avoided, and cost is remarkably reduced and efficiency is remarkably improved. The characteristics of short growth cycle and easy culture of tobacco are utilized to overcome the problems of low efficiency and low yield of traditional synthesis; positive transgenic tobacco leaves are dedifferentiated to form callus cells, so that materials are provided for large-scale fermentation, and an industrial production foundation is laid.

The present disclosure relates to a composition for balancing the immune system of a neonatal or infant subject, the composition comprising an effective amount of 2 '-fucosyllactose, 3-fucosyllactose, 3'-sialyllactose, 6 '-sialyllactose, and lactose-N-tetrasaccharide.

The invention relates to a gene modified cell for producing HMOs, which can be applied to synthesis of difucosyllactose, and belongs to the technical field of gene engineering. The invention provides a genetically modified cell, which contains an alpha-1, 2-fucosyltransferase polypeptide and a heterologous nucleic acid sequence of the alpha-1, 3-fucosyltransferase polypeptide. The genetically modified cell has higher activity of synthesizing difucosyllactose, and does not contain synthetic 3-fucosyllactose or lower side activity of synthetic 2 '-fucosyllactose. The technical scheme of the invention has positive significance for industrial production of human milk oligosaccharide, and the method is green, efficient and sustainable, is beneficial to industrial large-scale production, and has important practical value.

This powder material for a cosmetic contains a powder for a cosmetic that has been surface-treated with a surface treatment agent. The surface treatment agent contains: component (a), a mannosyl erythritol lipid represented by formula (1); and component (b), an ester compound of glycerin and a fatty acid having 8-20 carbon atoms. The content of component (a) is 0.5-3 mass%. The content of component (b) is 0.1-10 mass%.

The invention discloses an icaritin derivative, a composition and application, the structural formula of the icaritin derivative is as follows: in the structural formula, R1 is independently selected from hydrogen, hydroxyl and C1-C3 alkoxy; r2 is independently selected from hydrogen, an isopentenyl group, an isoamyl group and a Mannich base; r3 is independently selected from hydrogen, hydroxyl or OR3 ', R3 'is selected from rhamnosyl, rhamnose-(2-> 1)-glucosyl, rhamnose-(3-> 1)-glucosyl, propynyl and triazole groups substituted by alcoholic hydroxyl, benzyl and glycosyl; r4 is independently selected from a hydroxyl group or OR4 ', and R4' is selected from a propynyl group, a glucosyl group, and an alcoholic hydroxyl group, a benzyl group and a glycosyl substituted triazole group; r5 is independently selected from hydrogen, isopentenyl and Mannich base; mannich base is selected from N (CH3) 2 and N (CH2CH3) 2, R6 is independently selected from hydroxyl and OR6 ', and R6' is selected from hydrogen, rhamnosyl, rhamnose-(2-> 1)-xylosyl and rhamnose-(2-> 1)-rhamnosyl.The icaritin derivative provides a new direction for treatment of sarcopenia.

The invention belongs to the technical field of microorganisms, and particularly relates to application of bifidobacterium bifidum NKUFB3-12 and a composition thereof in preparation of products for relieving allergic diseases and / or regulating intestinal flora caused by allergy. The preservation number of the bifidobacterium bifidum NKUFB3-12 is GDMCC (China General Microbiological Culture Collection Center) No. 64013. The bifidobacterium bifidum NKUFB3-12 disclosed by the invention has excellent functional characteristics of relieving allergic diseases and regulating allergic intestinal flora, and also has excellent probiotic characteristics of acid resistance, cholate resistance and oxidation resistance, and the strain is safe and has no adverse side effect after being taken. The invention provides a composition. The composition comprises bifidobacterium bifidum NKUFB3-12 and 2 '-fucosyllactose (2'-FL), and the NKUFB3-12 is compounded with the 2 '-FL to show an effective inhibition effect on the imbalance of allergic immune response.

The present invention relates to nutritional compositions comprising digestible carbohydrates, lipids, proteins, 3-fucosyl lactose (3-FL) and a butyrate source, which are suitable for providing nutrition to an infant or young child suffering from or at risk of developing a food allergy, preferably cow milk protein or egg allergy, wherein the protein is or consists essentially of hydrolysed protein and / or free amino acids, and wherein the butyrate is provided in the form of dietary butyrate and / or by non-digestible saccharides selected from non-digestible polysaccharides, non-digestible oligosaccharides or mixtures thereof capable of undergoing microbial fermentation in the gastrointestinal tract to produce butyrate, and uses thereof.

The invention provides glycosyl transferase and application thereof in preparation of salidroside, and relates to the technical field of enzyme engineering. The glycosyl transferase FpUGT provided by the invention is derived from fraxinus pennsylvanica, the existence of the glycosyl transferase gene capable of directly catalytically synthesizing the salidroside in the plant is not reported in literatures, and a new biological way is provided for finding the glycosyl transferase for efficiently synthesizing the salidroside. And a new biological material is provided for preparing the salidroside.

The invention discloses a composition for preventing and / or treating alcoholic liver diseases, a product and application thereof. The present invention relates to a composition comprising: breast milk oligosaccharides and vitamins; the breast milk oligosaccharide comprises fucosyllactose and sialic acid lactose; the composition can provide nutritional support for patients with alcoholic liver diseases, repair intestinal mucosal barrier, reduce endotoxin entering blood, improve abundance of beneficial bacteria in intestinal tracts and reduce inflammatory response, so as to achieve the purpose of preventing and / or treating alcoholic liver diseases induced by alcohol.

The invention relates to a halogen-free heavy oil removal surfactant and a preparation method thereof, and belongs to the technical field of surfactants. The heavy oil removal surfactant is prepared from fatty alcohol-polyoxyethylene ether, hydrophilic alkyl quaternary ammonium salt, a glycosyl nonionic surfactant and an alcohol compound, the glycosyl nonionic surfactant is prepared by sequentially carrying out hydrophobic amidation and hydrophobic epoxy addition on lactobionic acid lactone; the heavy oil removal surfactant is good in oil removal effect, safe, free of halogen and small in corrosivity, and the use performance is improved through compound use of the fatty alcohol-polyoxyethylene ether, the hydrophilic alkyl quaternary ammonium salt and the glycosyl nonionic surfactant.

The invention discloses a hederacoside C deglycosylation derivative and a preparation method and application thereof.The hederacoside C deglycosylation derivative is prepared from hederacoside C through specific glycosidase hydrolysis, different products can be flexibly regulated and enriched on the basis of selectivity of different glycosidase to glucosidic bonds and glycosyl sites, the preparation method is simple, reaction conditions are mild, and the hederacoside C deglycosylation derivative is suitable for industrial production. The method is suitable for large-scale mass production. The hederacoside C deglycosyl derivative does not show obvious cytotoxicity to human immortalized keratinocytes, has the effect of promoting cell proliferation and the activity of promoting cell migration, and can obviously inhibit LPS (lipopolysaccharide)-induced cell apoptosis. Besides, the partial hederacoside C deglycosyl derivatives can reduce inflammatory factor level rise caused by wounds, remarkably promote wound healing and reduce formation of wound scars, and have good application prospects in preparation of drugs or skin care products for promoting wound repair.

The present application relates to a kind of 2'-fucosyllactose producing recombinant escherichia coli and its construction method and application, belong to the field of bioengineering technology.The present application expresses 2'-fucosyllactose remediation synthesis pathway related gene in recombinant escherichia coli, including the gene fkp of coding GDP-fucose pyrophosphorylase FKP and the gene futC of coding fucosyltransferase FutC, respectively in the upstream of fkp gene and futC gene expression macromolecular coagulation material protein FUSLCD after the modification of coagulation tag GCN4, and replaces replicon, constructs a strain of high-yield 2'-fucosyllactose recombinant escherichia coli, yield and production intensity reach 38.68g / L and 0.35g / L / h respectively.It has good application prospect, has the potential of industrial application.

The invention relates to the non-therapeutic nutricosmetic use of a composition in a form suitable for oral administration comprising a wheat extract in a physiologically acceptable medium. The invention is characterized in that the wheat extract contains 35-40% of triglycerides and diglycerides, 20-30% of glycolipids, 7-10% of phospholipids and 2-4% of ceramides and glycosylceramides, by weight relative to the total weight of the extract, and is used to reduce excessive sebum secretion in the skin of the face.

The application relates to a bacterial aggregation and killing bifunctional photosensitizer aggregate and a preparation method thereof. An AIE photosensitizer and a glycosyl amphiphilic molecule are added into a phosphate buffer solution according to a certain molar ratio, stirred uniformly, and then left to form the photosensitizer aggregate spontaneously. The preparation process of the photosensitizer aggregate constructed by the method is simple, convenient to operate, and has good repeatability. Experimental results prove that the constructed aggregate can induce bacterial aggregation, realize efficient killing of pathogenic bacteria, and further improve the sterilization efficiency when light is applied.

The present invention relates to an antifungal prodrug comprising an antifungal moiety linked to a trigger moiety by means of a self-immolative spacer. The trigger moiety is selected from a glycosyl residue and an oligosaccharide, stabilizing the self-immolative spacer and enabling cleavage by a pathogen hydrolase, preferably an extracellular glycosidase (EC 3.2.1). Upon cleavage of the trigger moiety by the pathogen hydrolase, the self-immolative spacer undergoes spontaneous degradation in order to release the antifungal moiety. The present invention further relates to pharmaceutical compositions comprising the prodrug and to the use thereof in the treatment of infectious diseases.

This application discloses a glutinous rice lime-based dry powder wall material and its preparation method. The wall material includes: hydrated lime; a modified glutinous rice adhesive powder system composed of α-pregelatinized glutinous rice starch and highly active metakaolin; and diatomaceous earth-supported glycosyl microparticles as a retarder and anti-caking agent. The preparation method includes: separately preparing the modified glutinous rice adhesive powder system and the diatomaceous earth-supported glycosyl microparticles, and then dry mixing the components. This application solves the technical problems of complex preparation, unstable storage, and poor durability of traditional glutinous rice lime slurry by loading hygroscopic glycosyl components into the micropores of diatomaceous earth and constructing an organic-inorganic dual-curing network, thus achieving industrialized production, convenient construction, long storage period, and good durability of the material.

The present disclosure provides mutated PglB oligosaccharyltransferase enzymes that have the ability to efficiently catalyze the transfer of a saccharide from a lipid carrier to an asparagine reissue in a glycosylation motif on a protein. Also provided are polynucleotides encoding the mutated PglB oligosaccharyltransferase enzymes, host cells capable of expressing the engineered PglB oligosaccharyltransferase enzymes, and methods of using the engineered PglB oligosaccharyltransferase enzymes to make N-glycosylated proteins. Also provided are N-glycosylated proteins that are made using the engineered PglB oligosaccharyltransferase enzymes.

The invention discloses beta-N-acetyl hexosaminidase Fmhen (I436S), the amino acid sequence of the beta-N-acetyl hexosaminidase Fmhen (I436S) is shown as SEQ ID NO.5, and the beta-N-acetyl hexosaminidase Fmhen is applied to synthesis of lactose-N-trisaccharide II. The invention also discloses a beta-N-acetyl hexosaminidase Fmhex (W291A), wherein the amino acid sequence of the beta-N-acetyl hexosaminidase Fmhex is shown as SEQ ID NO. 6. The invention also discloses an application of the mutant in synthesis of lactose-N-trisaccharide II. According to the invention, beta-N-acetyl hexosaminidase Fmhex is subjected to mutation modification to obtain two mutants with higher transglycosylation activity, namely Fmhex (W291A) and Fmhex (I436S), the conversion rates of the prepared lactose-N-trisaccharide II are respectively 1.68 times and 1.88 times of those of the beta-N-acetyl hexosaminidase Fmhex, and the beta-N-acetyl hexosaminidase Fmhex has higher application potential. The research of the invention has important industrial production value and economic value.

The invention discloses a disaccharide monosubstituted derivative of Lamellarin D and N. The structure of the disaccharide monosubstituted derivative is as follows: R1 and R2 are respectively and independently selected from one of H, methyl, benzyl, acetyl and disaccharide, R3 is selected from H, benzyl or disaccharide, R4 is selected from H, methylsulfonyl or disaccharide, and at least one of R1-R4 is disaccharide; the disaccharide group is one of cellobiose group, acetylated cellobiose group, lactosyl group, acetylated lactosyl group, maltose group and acetylated maltose group. According to the present invention, at least one disaccharide group is introduced into R1-R4 to obtain the disaccharide mono-substituted derivative of Lamellarin D and N, the proliferation inhibition activity of tumor cells is improved with the compound and the monosaccharide substituted derivative, and the inhibition effect on non-solid tumor cell line Jurkat is significantly improved by about 5 times;

This invention relates to a nitrogen-doped hard carbon material and its preparation method, belonging to the field of sodium-ion battery technology. The preparation method of this invention includes the following steps: S1, urea and a glycosyl carbon source are thoroughly mixed and heated to melt, then cooled to obtain a eutectic salt; S2, under a protective atmosphere, the eutectic salt is prepolymerized, pyrolyzed, and carbonized to obtain the nitrogen-doped hard carbon material. First, urea and a glycosyl carbon source form a eutectic salt. During this process, the strongly polar functional groups in the glycosyl carbon source form a hydrogen bond network with the amino and carbonyl groups in the urea. This hydrogen bond network can restrict the free movement of urea molecules and constrain their thermal decomposition path, thereby delaying and inhibiting premature decomposition of urea, while simultaneously improving the nitrogen retention rate. A three-stage heating program is used to achieve layered nitrogen doping. This segmented temperature control strategy constructs a layered doping mechanism of "first building a carbon framework, then introducing nitrogen atoms, and finally stabilizing the carbon framework structure."