1075 results about "Glycosyl" patented technology

The invention provides a genetically engineered bacterium for synthesizing fucosyllactose as well as a preparation method and application of the genetically engineered bacterium. The genetically engineered bacterium is prepared by knocking out a glucose-1-phosphate undecanoate transferase gene wcaJ and a beta-galactosidase gene lacZ on the basis of an original strain, the invention relates to an overexpression gene of a phosphomannose gene manB, a phosphoguanine transferase gene manC, a GDP-mannose-4, 6-dehydratase gene gmd, a GDP-fucose synthetase gene WcaG and a beta-galactoside permease gene lacY, and further, the overexpression gene of an alpha-1, 2-fucosyltransferase or an alpha-1, 3-fucosyltransferase is also used for the overexpression of a phosphomannose gene manB, a phosphoguanine transferase gene manC, a GDP-mannose-4, 6-dehydratase gene gmd, a GDP-fucosyltransferase gene. According to the invention, the modified Escherichia coli probiotic Nissle1917 is preferably used as a host for biosynthesis of fucosyllactose, no endotoxin hidden danger exists, and a foundation is laid for industrial production of fucosyllactose.

The invention relates to application of breast milk oligosaccharides to improvement of intestinal flora of mothers and infants, in particular to application of 2 '-fucosyllactose to preparation of food which is taken by a mother and contributes to regulation of intestinal flora of the mother and offspring thereof, and the mother is in a gestation period and / or a lactation period. The invention is provided through a large amount of research; when a mother supplements a certain amount of 2 '-fucosyllactose in a gestation period and / or a lactation period, the abundance of beneficial bacteria in intestinal tracts of the mother can be remarkably improved, the content of harmful bacteria is reduced, meanwhile, the abundance of beneficial bacteria in intestinal tracts of filial generations of the mother is remarkably improved, and the content of harmful bacteria is reduced; particularly, the abundance of some beneficial bacteria which cannot be directly eaten in the intestinal tracts of the mother and the offspring can be adjusted.

According to the glycosyl transferase mutant provided by the invention, the region selectivity of tyrosol is remarkably improved from 51% of a wild type to 99% or above, and when the glycosyl transferase mutant is applied to catalytic synthesis of salidroside, a single glycosylation product salidroside can be generated; besides, the catalytic activity of the microbial glycosyl transferase mutant to tyrosol is remarkably improved, the specific enzyme activity is improved to 97 mU / mg from 14 mU / mg of a wild type, and the substrate conversion rate is higher than 99%; according to the invention, the key problems of difficult glycosyl transferase expression, low regioselectivity and low activity in the salidroside preparation process are successfully solved, and the salidroside has a very great industrial application prospect.

The invention discloses a glycosyl transferase UGT76G1 mutant and a method for catalytically synthesizing rebaudioside M by using the glycosyl transferase UGT76G1 mutant, and relates to the technical field of biological catalytic synthesis. The glycosyl transferase UGT76G1 mutant is any one of the following components (A)-(C): (A) a protein obtained by performing any one or more of the following mutations on the basis of an amino acid sequence as shown in SEQ ID NO.1: the 89th amino acid is mutated into H from M; the 380th amino acid is mutated from L to M; the 411th amino acid is mutated from A to Y; (B) a protein which has 95% or 98% or more of identity with the amino acid sequence defined by (A) and has the same function as the amino acid sequence defined by (A); and (C) a fusion protein obtained by connecting a tag to the terminal of the protein defined in (A) or (B). The glycosyl transferase UGT76G1 mutant disclosed by the invention is obtained by mutating and screening glycosyl transferase UGT76G1, has higher catalytic activity, and can be used for efficiently synthesizing rebaudioside M by taking rebaudioside D and UDPG as substrates.

The invention provides infant formula milk powder for improving intestinal inflammation and / or microbial disorder as well as a preparation method and application of the infant formula milk powder. The invention provides infant formula milk powder for the first time, the infant formula milk powder comprises 2 '-fucosyllactose and lactose-N-neotetraose, and the mass ratio of the 2'-fucosyllactose to the lactose-N-neotetraose is (1-5.5): 1. The infant formula milk powder can improve intestinal inflammation and / or microbial disorder.

The invention relates to a gene modified cell and a method for producing fucosyllactose by using the gene modified cell, in particular to a saccharomyces cerevisiae gene modified cell and application thereof, and belongs to the technical field of biological gene engineering. The present invention provides a genetically modified cell comprising a recombinant nucleic acid sequence encoding a polypeptide having an [alpha]-1, 2-fucosyltransferase polypeptide ([alpha]-1, 2-fucosyltransferase) activity. The alpha-1, 2-fucosyltransferase polypeptide includes, but is not limited to, polypeptides having at least 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 99% and 100% identical amino acid sequences with the amino acid sequences of SEQ ID NO: 1-25. According to the gene modified cell, the fucosyllactose synthesis capability of an original strain is improved.

The invention discloses construction and optimization of a gene editing tool based on CRISPR (clustered regularly interspaced short palindromic repeats)-associated transsposases and application of the gene editing tool in escherichia coli, and belongs to the field of synthetic biology and gene engineering. According to the invention, an efficient gene editing system is established in Escherichia coli, and the burden of a MUSCULAR-CAST system on strains is reduced through element optimization; by eliminating the self-targeting effect, large-fragment (> 10k bp) target gene expression cassettes can be efficiently integrated, and the editing degree of the gene editing system is improved. According to the invention, a MUSCULAR-CAST gene editing system is used for constructing the chassis strains for producing 3-fucosyllactose, L-tryptophan, 2 '-fucosyllactose and neotetraose, so that the chassis strains have equivalent or higher expression yield compared with plasmids, and the growth condition is better or the conversion rate is higher.

The invention provides an application of citrus flavonoid glycosyl transferase CitUGT73A78 in biosynthesis of flavonoid glycoside, and belongs to the technical field of gene engineering and enzyme engineering. According to the present invention, the function of the CitUGT73A78 gene is cloned and verified, and the in vitro enzyme activity test proves that the CitUGT73A78 recombinant protein has broad-spectrum catalytic activity, and can efficiently catalyze the glucosylation of a variety of flavonoids so as to generate the corresponding flavonoids 7-O-glucoside and / or flavonoids 4 '-O-glucoside. An in-vivo transient overexpression test also proves that the CitUGT73A78 gene has a promoting effect on the accumulation of the content of the citrus flavonoid 7-O-glucoside. The invention not only provides a preparation method for the production of flavonoid 4 '-O-glucoside, has the advantages of simplicity, convenience, high catalytic efficiency, low cost, greenness and safety, but also can be applied to nutritional quality improvement and germplasm innovation of citrus fruits.

The invention provides an enzyme modification method based on artificial intelligence. The enzyme modification method comprises the following steps: training an AlphaFold2 model to obtain a glycosyltransferase tertiary structure prediction model; performing multi-sequence comparison on the protein and nucleic acid sequence database and the non-redundant protein feature sequence database based on the active point location recognition model to obtain a comparison result; initializing a matrix of which the length is equal to that of a comparison result, so as to construct a PSSM matrix and screen out potential mutation sites; according to the screened potential mutation sites, constructing an initial mutation library to form a virtual screening mutation library; performing structure reconstruction on the sequence in the virtual screening mutation library based on a glycosyl transferase tertiary structure prediction model so as to predict the glycosyl transferase tertiary structure and perform molecular docking and dynamic simulation on the glycosyl transferase tertiary structure; the method is used for analyzing binding pockets of proteins and ligands and determining dynamic interaction of amino acid residues and ligands in the binding pockets, and the contact number of each amino acid residue and the ligands is used for modifying the tertiary structure of the glycosyltransferase.

The invention belongs to the technical field of organic compounds, and particularly relates to a multifunctional organic silicon glycosyl amide cationic surfactant as well as a preparation method and application thereof. The preparation method of the surfactant comprises the following steps: carrying out amine ester reaction on glucolactone and N, N-dimethyl dipropylene triamine to obtain a precursor; carrying out substitution reaction on the precursor and bromododecane to obtain an intermediate; the intermediate and 3-chloropropyl methyl dimethoxy silane or 3-chloropropyl methyl trimethoxy silane are subjected to a quaternization reaction, and the surfactant is obtained. The surfactant has good environmental protection property and biocompatibility, is non-toxic and non-irritant, can sterilize and improve the rewettability of the material surface, and has wide application prospects in the aspects of pesticide, textile, daily chemical industry and material transportation and storage.

The present invention provides a combination comprising lactose and human milk oligosaccharides (HMOs) 2'-fucosyllactose (2'-FL), lacto- / V-neotetraose (LNnT), 3- fucosyllactose (3'-FL), at least one of 3'-sialyllactose (3'-SL) or 6'-sialyllactose (6'-SL), and optionally lacto-N-tetraose (LNT) for use in the dietary management of dysbiosis of the gut microbiota in a subject or for use in the dietary management of a disease or disorder associated with metabolism and / or accumulation of BCFA in a subject.

The present invention relates to a molecular imaging probe for tumor diagnosis, and provides a sugar nucleic acid molecular imaging probe, the probe comprises a DNA aptamer sequence and more than one glycosyl nucleoside module, and is labeled by radionuclide, the DNA aptamer sequence can specifically target tumor cells after functional folding; the glycosyl nucleoside module is a glycosyl modified nucleoside derivative, is accurately modified at the 5'end or / and the 3 'end of the aptamer through DNA solid-phase synthesis, has the imaging characteristics of biological safety, high sensitivity and high resolution, and has an excellent diagnosis effect on part of cancers which are difficult to diagnose by a traditional method. The invention also provides a preparation method and a use method of the sugar nucleic acid molecular imaging probe, so that sugar modification of the aptamer is convenient, rapid, accurate, controllable and modularized, and the sugar nucleic acid molecular imaging probe has huge clinical application potential and transformation value in cancer diagnosis.

A surfactant system comprising an anion surfactant, a fatty alcohol ethoxylate and a mannosyl erythritol lipid can be used in detergents, cleaning or textile care products and has improved detergent power.

The invention belongs to the field of nutrient substance research, and particularly relates to application of a nutrient composition to regulation of brain immunity. The invention provides application of a nutritional composition in preparation of food for regulating brain immunity. Wherein the nutritional composition comprises the following necessary components: lactoferrin and fucosyllactose, and in the nutritional composition, the mass ratio of the lactoferrin to the fucosyllactose is 1: (0.1-30). Research finds that after lactoferrin and fucosyllactose are combined according to a certain proportion, the lactoferrin and fucosyllactose have a synergistic effect of regulating brain immunity. Experimental data show that after the combination of lactoferrin and fucosyllactose is used for treating the BV2 cells, the trend that the BV2 cells are differentiated into M2 phenotype microglial cells is higher than the trend that the BV2 cells are differentiated into M1 phenotype microglial cells, and the amount of secreted inflammatory factors is obviously reduced.

The invention relates to 2 '-fucosyllactose, in particular to application of the 2'-fucosyllactose to preparation of immune balance drugs or food. The immune balance comprises regulation and control of the ratio of Th17 cells to Treg cells of Th17 / Treg immune imbalance offspring activated by maternal immune or induced by virus infection; according to the application disclosed by the invention, the fact that filial generations generated after the maternal body of a mammal generates immune reaction activation can regulate and control the proportion of Th17 and Treg cells through the influence of 2 '-fucosyllactose on intestinal flora is found, so that Th17 and Treg immune balance is realized; in addition, social and cognitive disorders and repetitive behaviors related to filial generation autism induced by Th17 / Treg immune imbalance can be improved.

The present invention relates to a combination of a mixture of galactooligosaccharides (GOS) and human milk oligosaccharides (HMO), or a nutritional composition comprising such a combination. The combination or composition is useful for the treatment and / or prevention of stress and / or mood disorders in infants, toddlers and / or children. The mixture of HMO consists of or consists essentially of 2 '-fucosyllactose (2' FL) and difucosyllactose (DFL) or lactose-N-tetrasaccharide (LNT) and 6 '-sialyllactose (6' SL). The invention also relates to the use of a combination of a mixture of galactooligosaccharides (GOS) and human milk oligosaccharides (HMO) or a composition comprising such a combination for the treatment and / or prevention of stress and / or mood disorders in infants, toddlers and / or children, wherein the mixture of HMO consists of or consists essentially of 2 '-fucosyllactose (2' FL) and difucosyllactose (DFL) or lactose-N-tetrasaccharide (LNT) and 6 '-sialyllactose (6' SL).

The invention discloses a solid composite carbon source for sewage denitrification and a preparation method thereof. The composite carbon source is prepared from the following components in parts by weight: 10 to 60 parts of sodium acetate, 10 to 30 parts of glucose, 10 to 30 parts of starch, 0.1 to 2 parts of yeast powder, 1 to 5 parts of cellulose and 0.1 to 0.3 part of glycosyl hydrolase. Sodium acetate in the composite carbon source can react quickly, an instantaneous carbon source is provided, glucose and starch can be utilized by microorganisms for a long time, and durability of the carbon source is guaranteed. The invention also provides a sewage treatment method using the composite carbon source, and the method comprises the following steps: adding the composite carbon source into sewage, and carrying out denitrification treatment. The addition amount of the composite carbon source is determined based on the C / N ratio in the sewage. By adding the composite carbon source, the content of nitrate nitrogen in sewage can be effectively reduced, so that the sewage treatment efficiency is improved. The composite carbon source exists in a solid form, is convenient to store and transport, and reduces the influence on the environment. The composite carbon source not only can obviously improve the sewage treatment effect and improve the effluent quality, but also can reduce the operation cost and additional cost, and has important significance for improving the environment.

The invention discloses a composition for improving bone density and application thereof, and belongs to the technical field of food. According to the invention, the 1, 3-dioleic acid-2-palmitin, the osteopontin and the oligosaccharide are compounded for use, and the components interact to promote mutual absorption and utilization, so that the growth and development of bones in the growth period of infants are improved, the mineral content and the bone mineral density of the bones are increased, the health of the bones is promoted, and the composition is safe and free of side effects. And by optimizing the variety of the oligosaccharide, the compound use of the galactooligosaccharide and the 2 '-fucosyllactose is found to be capable of better improving the growth and development of bones in the growth period of the infants.

The invention belongs to the technical field of nutritious food, and discloses a composition for simulating whole breast milk oligosaccharide and application of the composition. In the composition for simulating the whole breast milk oligosaccharide, the mass ratio of 2 '-fucosyllactose to lactose-N-neotetraose to 3'-sialyllactose is (3.9-8.1): (0.8-1.2): (0.8-1.2), and the breast milk oligosaccharide 2 '-fucosyllactose, the lactose-N-neotetraose and the 3'-sialyllactose are mutually matched to take effect in a synergistic manner, so that the whole breast milk oligosaccharide is obtained. The composition can improve the memory ability and cognitive ability of an eating object, can be added into infant food (including infant formula powder, complementary food and nutritional supplements) and nutritional supplements or food of children over 3 years old, teenagers and aged people, and has a wide application prospect.

The invention belongs to the technical field of food, and relates to application of breast milk oligosaccharide in regulating brain immunity. Specifically, the invention relates to the non-therapeutic purpose of adjusting brain immunity by adjusting polarization of microglial cells M1 / M2, and the breast milk oligosaccharide is any one of 3-fucosyllactose, lactose-N-tetraose, lactose-N-neotetraose, 3 '-sialic acid lactose and 6'-sialic acid lactose. Research results show that a specific variety of breast milk oligosaccharides (especially 3-FL) can obviously inhibit the generation of neurotoxic factors NO, inflammatory factors TNF-alpha and IL-1beta in microglial cells, and promote the generation of anti-inflammatory cytokines IL-10 in the microglial cells, so that the state of the microglial cells is improved, damage repair is promoted, and the microglial cells are prevented from being damaged. Therefore, the effect of regulating brain immunity is achieved.

The invention provides essential oil containing ceramide and a preparation method of the essential oil. The essential oil containing ceramide is prepared from ceramide, mannosyl erythritol lipid, synthetic grease and natural vegetable grease. According to the ceramide-containing essential oil disclosed by the invention, complex components such as a surfactant and a stabilizer do not need to be added, and the added mannosyl erythritol lipid is natural sugar ester and has good biocompatibility and safety, so that the ceramide-containing essential oil disclosed by the invention is mild and non-irritant to skin and has excellent product safety; besides, according to the pure oil formulation formula, the purpose of improving the solubility and stability of ceramide can be achieved only by adding mannosyl erythritol lipid, the skin barrier maintaining effect of ceramide can be better played, the anti-inflammatory and skin repairing effects of ceramide are improved, and the skin health level is improved; the application range of ceramide in cosmetics is expanded.

The invention provides a composition for promoting bone health and bone formation as well as application and a product thereof, and belongs to the field of foods. The composition disclosed by the invention is prepared from the following components in parts by weight: 1 to 5 parts of osteopontin, 1 to 50 parts of 2 '-fucosyllactose and 0.05 to 50 parts of a calcium source. The composition disclosed by the invention shows a relatively good synergistic effect in promoting calcium absorption, and can be used for improving bone density, preventing and treating osteoporosis and the like.

The invention relates to the technical field of gene engineering, in particular to a glycosyl transferase mutant, a sucrose synthase mutant and application of the glycosyl transferase mutant and the sucrose synthase mutant in synthesis of rebaudioside M. Wild glycosyl transferase and wild sucrose synthase are modified according to a directed evolution theory to obtain a glycosyl transferase mutant and a sucrose synthase mutant. Rebaudioside M is obtained by catalyzing rebaudioside D through a glycosyl transferase mutant and sucrose synthase mutant co-expression strain, and the catalytic efficiency is superior to that of a wild enzyme. The rebaudioside M is obtained by completely catalyzing 110 g / L of rebaudioside D through the co-expression strain of the glycosyl transferase mutant and the sucrose synthase mutant, only 20 h is needed, the conversion rate reaches 97%, the yield of the rebaudioside M reaches 122 g / L, the raw material conversion rate and the yield of the rebaudioside M are effectively increased, and meanwhile the synthesis time is shortened.

The invention relates to the technical field of oilfield chemistry, in particular to a glycosyl anionic surfactant and a preparation method and application thereof, and the glycosyl anionic surfactant is prepared from glucose, long-chain alkyl primary amine and succinic anhydride sodium hydroxide. The glycosyl anionic surfactant has excellent surface activity, is simple in preparation method, high in product yield, safe and reliable, can be used for oil displacement agents, polymer surface oil displacement agents, emulsifiers, discharge aiding agents, heavy oil viscosity reducers and the like, is wide in application, and meets the green development requirements of oil fields. The acid fracturing discharge aiding agent using the glycosyl anionic surfactant as a raw material can effectively reduce the surface and interfacial tension of an aqueous solution, and meets the technical specification of the acid fracturing discharge aiding agent.

The invention provides a children formula food for improving intestinal inflammation and / or microbial disorder as well as a preparation method and application of the children formula food. The invention firstly provides the infant formula food, the infant formula food is infant formula food or child formula food, the infant formula food comprises 2 '-fucosyllactose and lactose-N-neotetraose, and the mass ratio of the 2'-fucosyllactose to the lactose-N-neotetraose is (1-5.5): 1. The children formula food can improve intestinal inflammation and / or microbial disorder.

The invention discloses a preparation method of glucosyl stevioside, and relates to the technical field of glucosyl stevioside production, rebaudioside A or stevioside is taken as a raw material, cyclodextrin is taken as an auxiliary material, cyclodextrin glucosyltransferase is added for glycosylation reaction, a glucosyl stevioside solution I is cooled, and a glucosyl stevioside solution II is obtained; the method comprises the following steps: adding sucrose, UGT glycosyl transferase, sucrose synthase and uridine diphosphate into rebaudioside A or stevioside, continuously converting unreacted rebaudioside A or stevioside into rebaudioside D or rebaudioside E, inactivating after the reaction is finished, filtering by a plate frame, removing impurities through resin, analyzing the resin by using ethanol to obtain an analysis solution, and treating the analysis solution at 78-82 DEG C for 2-4 hours to obtain the rebaudioside D or rebaudioside E; and dealcoholizing at the vacuum degree of-0.1 MPa, concentrating and drying to obtain the glucosyl stevioside. The quality of the product is improved, the cost is reduced, and the taste of the product is integrally improved.

The invention relates to an ardisia crenata carbon glycosyl transferase AcCGT64 gene and application of the ardisia crenata carbon glycosyl transferase AcCGT64 gene in preparation of bergenin, and belongs to the technical field of biology. The nucleotide sequence of the ardisia crenata carbon glycosyl transferase AcCGT64 gene is as shown in SEQ ID NO. 1, and the total length of the sequence is 1359 bp; the amino acid sequence of the encoded protein is as shown in SEQ ID NO.2, and 451 amino acid residues are encoded. The ardisia crenata carbon glycosyl transferase AcCGT64 gene disclosed by the invention can be used as a biosynthesis regulation gene of bergenin, is applied to preparation of bergenin, and is remarkable in application prospect and easy to popularize and apply.

The invention relates to a sample treatment method for selectively removing glycosyl from glycosphingolipid through photocatalysis and a mass spectrometric detection method. The sample treatment method specifically comprises the following steps: (1) dissolving a sample in an alkaline protic solvent; (2) dispersing titanium dioxide particles; and (3) carrying out photocatalytic reaction under ultraviolet irradiation, and centrifuging to obtain a sample solution subjected to photocatalytic treatment. The mass spectrometric detection method specifically comprises the following steps: injecting a sample solution subjected to photocatalysis treatment into a mass spectrum, carrying out primary mass spectrometric detection, and carrying out comparison data analysis on the sample solution subjected to photocatalysis treatment and a sample extracting solution not subjected to photocatalysis treatment, a primary mass spectrum peak which is possibly a glycolipid decomposition product is screened through comparison and analysis results, and secondary mass spectrum detection is further carried out, so that the accurate molecular structure of glycolipid is analyzed. According to the method, the glycosyl head of glycolipid can be selectively, rapidly and efficiently removed in the presence of complex lipidomes, subsequent mass spectrum detection analysis is carried out, glycolipid spectrum peaks in complex mass spectrum peaks can be effectively, rapidly and accurately found out by comparing the mass spectrum peaks before and after removal, and the detection result is accurate. Important chemical information is provided for rapid locking of correct candidate substances in the first-level mass spectrum qualitative process, and meanwhile, the glycolipid decomposition product can obtain carbon chain structure information which is richer than that of original glycolipid to assist qualitative analysis.

According to the invention, a glycosyl transferase mutant derived from Bacillus licheniformis ZSP01 is used as a research object, AlphaFold 2 is used for constructing a three-dimensional structure of a protein, then technologies such as molecular docking and molecular dynamics simulation are used for analyzing amino acids far away from a catalytic center in glycosyl transferase, site-directed mutagenesis is carried out on the amino acids, and the glycosyl transferase is obtained. The activity of the mutant for catalytically synthesizing the icariside D2 glycosyltransferase is improved.

The invention belongs to the field of food, and particularly relates to a nutritional composition of oligosaccharide and probiotics and application of the nutritional composition. The invention provides application of a nutritional composition in preparation of food beneficial to improvement of constipation and improvement of cognitive competence decline caused by constipation. Wherein the nutritional composition comprises the following components (i) and (ii): (i) a neutral fucosylated breast milk oligosaccharide, and (ii) a bifidobacterium probiotic; wherein the neutral fucosylated breast milk oligosaccharide at least comprises 2 '-fucosylated lactose, and the bifidobacterium probiotics at least comprise bifidobacterium longum subsp. Longum. According to the present invention, with the substance combination of the neutral fucosylated breast milk oligosaccharide and the bifidobacterium probiotic, the abnormal brain-intestine interaction can be improved, such that the constipation can be easily improved, and the cognitive ability reduction caused by the constipation can be easily improved;