62 results about "Glycosyl" patented technology

The present invention relates to nutritional compositions comprising digestible carbohydrates, lipids, proteins, 3-fucosyl lactose (3-FL) and a butyrate source, which are suitable for providing nutrition to an infant or young child suffering from or at risk of developing a food allergy, preferably cow milk protein or egg allergy, wherein the protein is or consists essentially of hydrolysed protein and / or free amino acids, and wherein the butyrate is provided in the form of dietary butyrate and / or by non-digestible saccharides selected from non-digestible polysaccharides, non-digestible oligosaccharides or mixtures thereof capable of undergoing microbial fermentation in the gastrointestinal tract to produce butyrate, and uses thereof.

The present invention relates to an antifungal prodrug comprising an antifungal moiety linked to a trigger moiety by means of a self-immolative spacer. The trigger moiety is selected from a glycosyl residue and an oligosaccharide, stabilizing the self-immolative spacer and enabling cleavage by a pathogen hydrolase, preferably an extracellular glycosidase (EC 3.2.1). Upon cleavage of the trigger moiety by the pathogen hydrolase, the self-immolative spacer undergoes spontaneous degradation in order to release the antifungal moiety. The present invention further relates to pharmaceutical compositions comprising the prodrug and to the use thereof in the treatment of infectious diseases.

This application discloses a glutinous rice lime-based dry powder wall material and its preparation method. The wall material includes: hydrated lime; a modified glutinous rice adhesive powder system composed of α-pregelatinized glutinous rice starch and highly active metakaolin; and diatomaceous earth-supported glycosyl microparticles as a retarder and anti-caking agent. The preparation method includes: separately preparing the modified glutinous rice adhesive powder system and the diatomaceous earth-supported glycosyl microparticles, and then dry mixing the components. This application solves the technical problems of complex preparation, unstable storage, and poor durability of traditional glutinous rice lime slurry by loading hygroscopic glycosyl components into the micropores of diatomaceous earth and constructing an organic-inorganic dual-curing network, thus achieving industrialized production, convenient construction, long storage period, and good durability of the material.

This invention relates to a nitrogen-doped hard carbon material and its preparation method, belonging to the field of sodium-ion battery technology. The preparation method of this invention includes the following steps: S1, urea and a glycosyl carbon source are thoroughly mixed and heated to melt, then cooled to obtain a eutectic salt; S2, under a protective atmosphere, the eutectic salt is prepolymerized, pyrolyzed, and carbonized to obtain the nitrogen-doped hard carbon material. First, urea and a glycosyl carbon source form a eutectic salt. During this process, the strongly polar functional groups in the glycosyl carbon source form a hydrogen bond network with the amino and carbonyl groups in the urea. This hydrogen bond network can restrict the free movement of urea molecules and constrain their thermal decomposition path, thereby delaying and inhibiting premature decomposition of urea, while simultaneously improving the nitrogen retention rate. A three-stage heating program is used to achieve layered nitrogen doping. This segmented temperature control strategy constructs a layered doping mechanism of "first building a carbon framework, then introducing nitrogen atoms, and finally stabilizing the carbon framework structure."

Provided is an adjuvant composition comprising a glycoarchaeol and at least one immunostimulant selected from a Toll-like receptor (TLR) agonist and a saponin. The glycoarchaeol and / or immunostimulant may be present as a pharmaceutically acceptable salt. The adjuvant composition may be comprised together with an antigen in an immunogenic composition, such as a vaccine composition, which may be for use to induce an immune response in a subject. Further provided is use of the immunogenic composition to induce an immune response in a subject, particularly an immune response that comprises both a cell-mediated response and a humoral response.

The present application provides a nutritional composition for improving intestinal inflammation and microbial imbalance and application thereof. Specifically, the present application provides application of a composition in preparation of a product for improving intestinal inflammation and / or microbial imbalance, wherein the composition comprises 2'-fucosyllactose and lacto-N-neotetraose, and the mass ratio of 2'-fucosyllactose to lacto-N-neotetraose is (1-5.5):1. The present application also provides a food containing the composition, and application of the composition or the food in preparation of a product for improving intestinal inflammation and microbial imbalance.

Provided herein is a noninvasive method for measuring pH, glucose and glycogen levels using a single acquisition method, as well as systems for performing the method. This method for glycogen / glucose (and pH) detection has high specificity enabled by superior chemical shift separation in high-strength magnetic fields. The methods have applications in detecting changing tumor microenvironments in cancerous cells for measuring important metrics for noninvasive and quantitative metabolic profiling.

The present disclosure relates to gypsum slurries. In one aspect, a surfactant combination containing (i) 20 wt% to 80 wt% of an anionic diaryl oxide surfactant; and (ii) 20 wt% to 80 wt% of a non-ionic glycosyl surfactant can be used as a foaming agent for gypsum slurries used to produce gypsum board. The minimum ratio of anionic surfactant to non-ionic surfactant can vary depending on the lipophilic portion of the anionic surfactant. The resulting board can have a desired combination of weight and physical strength, as evidenced by nail pull resistance.

The application discloses a diabetes patient biological age calculation method based on glycosylation markers and a multi-task deep learning framework, and belongs to the technical field of bioinformatics and medical data processing technology, and the method comprises the following steps: obtaining glycomics feature data and clinical examination data of diabetes patients; preprocessing the data; obtaining a feature set by adopting a multi-method screening and fusion strategy; constructing a deep learning model comprising a multi-modal feature fusion module and a multi-task learning module for training, wherein the multi-task learning module comprises a main task of aging clock regression and at least one auxiliary task; and outputting biological age prediction values and age acceleration evaluation results of individuals by using the trained model. The method realizes deep fusion of multi-modal biological data and exclusive modeling for the diabetes population, can accurately quantify the biological aging process and the deviation degree relative to the calendar age, and provides an objective quantitative tool for clinical evaluation.

The application provides a composition for promoting growth of Akkermansia and application, the composition comprises colostrum basic protein, beta-glucan and 2'-fucosyllactose, wherein, in the composition, the concentration ratio of the colostrum basic protein, beta-glucan and 2'-fucosyllactose is (0.025-0.3):(0.03-0.15):(0.6-2.5). The composition can fully meet the nutritional requirements of Akkermansia bacteria, especially mucinophilic Akkermansia bacteria, can effectively promote the growth of Akkermansia bacteria, can be used for culturing Akkermansia bacteria in vitro, and can promote the growth of mucinophilic Akkermansia bacteria in human intestinal tract, increase the potential probiotic Akkermansia bacteria in the intestinal tract, and improve intestinal health.

Provided in the present application are a glycosylated fusion protein, a nucleic acid molecule, an expression vector, a host cell and the use thereof. A first aspect of the present application provides the glycosylated fusion protein, comprising a murine Fc variant and a hepatitis B virus antigen, wherein the murine Fc variant is obtained by performing amino acid mutation and non-mammalian glycosylation modification on a murine Fc fragment, the murine Fc variant comprises at least one of alanine at position 223, alanine at position 228, alanine at position 230, leucine at position 330, and glutamic acid at position 332, the glycosylation modification does not comprise sialic acid modification, and the positions are numbered according to the EU numbering system. The fusion protein can bind to DC cells and activate DC cells, and thus promote the proliferation and activation of specific T cells.

The invention belongs to the technical field of biology, and particularly relates to a method for detecting and enriching glycosylated RNA (Ribonucleic Acid) based on in-vitro enzyme chemical labeling of alpha-2, 3, alpha-2, 6 type sialyltransferase. According to the application, sialidase is firstly used for digesting sialic acid at the tail end of a naturally generated glycoRNA sugar chain, and sialidase digestion is not needed for an RNA sample which does not have sialic acid at the tail end of the glycoRNA sugar chain but has potential sialic acid modification sites; sialic acid with a labeled group is transferred to a potential sialic acid modification site at the tail end of a glycoRNA carbohydrate chain by using alpha-2, 3, alpha-2, 6 type sialyltransferase, such as PmST1, PmST3, Ph2, 3siat and Pd26ST, so that glycoRNA with a detectable group can be directly obtained, or the detectable group is further introduced through subsequent reaction, so that subsequent signal detection or enrichment can be carried out; the RNA part can be further sequenced, and the glycosyl part can be further subjected to mass spectrum identification. The application provides a very efficient, simple and convenient detection means for detection and enrichment of sialylated glycoRNA, has the characteristics of high specificity and high sensitivity, and has a wide application prospect.

The present invention relates to C-glycosyl aryl compounds and their use for protecting the heart and kidney. In particular, the compounds of the invention have the structure of Formula I, wherein the definitions of the various groups and substituents are as described in the specification. Methods for preparing the compounds and their use for protecting the heart and kidney are also disclosed.

This invention relates to the field of intelligent detection technology in bio-fermentation, specifically a method for detecting samples from the fermentation process of 2'-fucosylated lactose. The method involves collecting 2'-fucosylated lactose fermentation broth from the fermenter via an automated aseptic sampling interface. This interface includes a micro-flow sensor, a control pump, and a pressure sensor. The pressure sensor monitors the transmembrane pressure difference across a filter membrane in real time, while the micro-flow sensor monitors the sampling flow rate and provides feedback to control the pump speed, ensuring the sampling flow rate remains stable within a set range. The collected 2'-fucosylated lactose fermentation broth is centrifuged and filtered, then diluted with a diluent to obtain the sample solution to be tested. This sample solution is injected into a high-performance liquid chromatography (HPLC) system and an online near-infrared spectroscopy (NIRS) system for detection. The HPLC system, under elution conditions, determines the concentrations of 2'-fucosylated lactose, lactose, glucose, and sucrose. The NIRS system uses a pre-established partial least squares regression model to predict the concentrations of each component in real time.

A substantially anhydrous, concentrated surfactant composition comprising a sugar- based surfactant; an amino acid based surfactant; a polyglycerol ester a polyhydroxy ether, or a combination of a polyglycerol ester a polyhydroxy ether; and, optionally, a glycol, wherein the substantially anhydrous surfactant composition contains less than about 12 weight percent of water.

Provided are two novel endo-beta-N-acetylglucosaminidases, as well as a method for using the two with a disaccharide linker to prepare a non-natural sugar-engineered antibody, or a fixed-point quantitative antibody drug conjugate based on glycosyl modification, and a use.

This invention belongs to the field of hydrogel technology, and relates to an oxygen-initiated free radical polymerization hydrogel, its preparation method, and its applications. The method, conducted in an air atmosphere, uses monomers, sodium glycosyl sulfinate, and a crosslinking agent as reaction components. Dissolved oxygen in the reaction system or ambient oxygen serves as the free radical initiator, achieving rapid in-situ gelation of the hydrogel over a wide pH range and reaction temperature conditions. It is applicable to various monomer systems. The hydrogel prepared using this method exhibits good mechanical properties and structural stability, meeting the requirements for rapid gelation in open environments. It can be applied in fields such as biomedical materials, wearable electronic devices, bioprinting, and on-site remediation. This invention breaks through the traditional understanding of oxygen as a polymerization inhibitor in free radical polymerization, proposing a novel rapid gelation strategy for hydrogels using oxygen as the initiator.

This invention discloses a glycosyl carbon quantum dot oil displacement agent for unconventional oil reservoirs, its preparation method, and its application, belonging to the field of oilfield chemical technology. The preparation of the glycosyl carbon quantum dot oil displacement agent for unconventional oil reservoirs of this invention includes: adding a carbon source and a modifier to an alcohol solution, stirring to dissolve, and then carrying out a hydrothermal reaction to obtain the product; wherein the modifier is a ketoxime modifier; this method is simple, low-cost, has controllable particle size, and high yield, making it suitable for industrial application; the obtained carbon quantum dots have a high specific surface area and functional groups such as carboxyl and hydroxyl groups, which can penetrate nanopores and synergistically reduce the oil-water interfacial tension to below 5.0 mN / m; this product has outstanding high-temperature resistance and penetration ability, and can effectively penetrate into the nanopore throat structure of tight reservoirs, significantly improving oil displacement efficiency through interfacial modification, and is particularly suitable for the exploitation of unconventional nano-oil and gas resources.

This invention belongs to the field of cosmetics, specifically relating to a highly adherent foundation makeup composition and its preparation method. The highly adherent foundation makeup composition comprises an emulsifier phase, a thickener phase, a functional powder phase, and an oil phase; wherein the emulsifier phase comprises one or more polyglycerol fatty acid esters, the thickener phase comprises one or more modified bentonite and / or glycosyl fatty acid esters, the functional powder phase comprises one or more mica, silica, and zinc oxide, and the oil phase comprises one or more fatty acid esters. The foundation makeup composition obtained by this invention has advantages such as good system stability, uniform powder dispersion, good spreadability, even and delicate application, high adherence, and resistance to powder settling and patchiness, and is suitable for both water-based and waterless foundation makeup products.

Described herein are novel methods of preparing sphingosines and glycosylated sphingosines. A method of preparing a glycosylated sphingosine as described herein includes an efficient, four-step route for synthesizing lactosylsphingosine (LacboSph) from Garner's aldehyde. Also described herein is a cost-effective method of synthesizing a variety of glycosylated sphingosines, sphingosines and glycosyl acceptors, including both the d18:1 and d20:1 D-erythro-sphingosines and the d18:1 and d20:1 D-erythro-sphingosine glycosyl acceptors, from xylose.

This invention discloses a fully humanized N-glycosylated yeast strain and its applications. The modification of the yeast strain in this invention includes the knockout of basal N-glycosylation, the construction of a humanized N-glycosylation pathway, the integration of genes related to heparin precursor synthesis, and the integration of genes for heparin-modifying enzymes. The N-glycosylated Pichia pastoris platform strain described in this invention represents the first time that full humanization has been achieved, with its N-glycosyl group including fucosylate. When expressing animal-derived heparin-modifying enzymes, the fully humanized Pichia pastoris platform strain of this invention exhibits increased enzyme activity, thereby increasing the sulfate groups in the heparin glycan chains, thus enhancing the biological activity of heparin.

The application belongs to the technical field of biological medicine, and discloses a preparation method and application of an antibody drug conjugate with site-specific glycosyl coupling. The application discloses a recombinant cell containing a nucleic acid molecule encoding trastuzumab, and the FUT8 gene and the B4GALT1 gene are deleted. The application provides a recombinant cell which can efficiently and stably prepare trastuzumab with a uniform A2 glycosylation type. Based on the trastuzumab, 2, 4 or 6 azido groups can be introduced at specific sites of the antibody by using alpha 1, 6-fucosyltransferase and / or beta 1, 4-galactosyltransferase, and a toxin molecule is further connected, so that an antibody conjugate with uniformity and DAR value = 2, DAR value = 4 or DAR value = 6 is prepared.

The invention relates to a composition based on enzymes of the class of glycosyl hydrolases. The given composition can be used as a dishwashing composition, especially for removal of tough stains and soils based on hemicellulose residues of shells of cereals, plants and fungi even at lower temperatures.

The invention discloses a platycodon grandiflorum phosphomannose isomerase gene PgPMI as well as an encoded product and application thereof, and belongs to the technical field of platycodon grandiflorum genes. According to the invention, the platycodon grandiflorum phosphomannose isomerase gene PgPMI is cloned from platycodon grandiflorum, and the nucleotide sequence of the platycodon grandiflorum phosphomannose isomerase gene PgPMI is shown as SEQ ID NO. 1. According to the invention, the expression of the PgPMI gene in escherichia coli is successfully realized, and an enzymatic experiment verifies that the PgPMI has catalytic activity, so that mannose-6-phosphoric acid and fructose-6-phosphoric acid can be mutually converted, and a foundation is laid for revealing biosynthesis of platycodon grandiflorum polysaccharide. In addition, the platycodon grandiflorum phosphomannose isomerase can also be applied to biosynthesis pathways of plant ascorbic acid, cell walls, glycosylated proteins, lipoproteins, glycosyl phosphate inositol ankyrin and the like.

The present invention provides a combination of vitamin mixtures and synbiotics for use in enhancing bone growth and / or bone strength, for example, in children suffering from and / or developmental delay.

The invention belongs to the field of chemical synthesis, and relates to glycosyl thioether with oxime functional groups and a preparation method and application thereof. According to the invention, glycosyl thioether with oxime functional groups is prepared, the glycosyl thioether is used as a glycosyl free radical donor, a styrene compound is used as a glycosyl acceptor substrate, and a method for synthesizing alkyl C-glycoside through visible light catalysis is established and is used for synthesizing a series of C-glycoside compounds. The oxime-bonded glycosyl thioether prepared by the invention is stable in property, the preparation method has the advantages of simplicity, convenience, high yield, wide applicable saccharide range and the like, and the oxime-bonded glycosyl thioether is applied to synthesis of carbon glycoside and has the advantages of simplicity, wide substrate application range, including various drugs and polypeptides and the like. Therefore, the glycosyl thioether provides a new thought for development of complex glycoconjugates and drugs, and further promotes industrial development of carbon glycoside medicines.

The present invention provides a combination of lactose and at least one of the human milk oligosaccharides (HMOs) 2'-fucosyl lactose (2'-FL), lacto- / V-neotetraose (LNnT), 3-fucosyl lactose (3'-FL), 3'-sialyl lactose (3'-SL), or 6'-sialyl lactose (6'-SL), and optionally lacto-N-tetraose (LNT), for use in dietary management of intestinal microbiota dysbiosis in a subject, or for use in dietary management of diseases or disorders related to the metabolism and / or accumulation of BCFAs in a subject.

The present invention provides a cosmetic composition that can maintain high moisturizing properties even after being washed off with water. [Solution] The cosmetic composition of the present invention contains a biosurfactant having a mannose skeleton and a water-retaining agent. For example, the water-retaining agent is a sugar alcohol, rare sugar, isomerized sugar, alkyl glycoside, sucrose fatty acid ester, or hyaluronic acid and derivatives thereof, and the biosurfactant contains mannosylerythritol lipid (MEL).