315 results about "Natural source" patented technology

The invention discloses a method for efficiently separating and purifying plant exosomes and application, and belongs to the technical field of biological nanotechnology. The method comprises the following key steps: (1) performing sterile cleaning on plant raw materials, and then performing peeling or tissue slitting pretreatment; (2) carrying out mechanical crushing treatment by adopting a phosphate buffer solution (PBS), and filtering through a 200-mesh screen to obtain a primary extracting solution; (3) performing gradient centrifugal purification on the extracting solution, collecting supernate, and filtering the supernate through a 0.45 mu m microporous filter membrane; (4) adding 8-12% of polyethylene glycol for low-temperature phase separation and precipitation; (5) purifying by using an electrodialysis technology; and (6) carrying out final concentration by adopting a 100kDa ultrafiltration membrane to obtain the high-purity PELNs preparation. According to the method, the process, equipment and reagents are simple and easy to obtain, the final yield is high, the purity is high, particle size distribution is uniform, and an innovative solution is provided for large-scale industrial production of natural-source nano-drug carriers.

The invention discloses a beta-glucuronidase inhibitor composition as well as a preparation method and application thereof. The composition is prepared from the following substances: ursolic acid, procyanidine, icariin, curcumin, epigallocatechin gallate and glycyrrhetinic acid. The beta-glucuronidase inhibitor composition has a broad-spectrum and good inhibition effect, the preparation method is simple, large-scale production is easy, and the inhibitor with a natural source is higher in use compliance, lower in toxic and side effects and more suitable for developing intestinal health products and has an application prospect.

Compositions of matter, methods, and systems for producing fibers, yarns, and fabrics with enhanced thermoregulation properties are provided. The composition may be created from a cellulose solution derived from natural sources, into which nano-sized phase change materials and high-specific heat minerals are incorporated. These additives form a mesoporous structure within the fiber, enabling the capture and retention of the nanomaterials. The method involves spinning this composite solution into yarn, which can be blended with additional fibers to produce a bespoke yarn. This yarn can then be knitted or woven into fabrics that maintain thermoregulation.

The invention discloses a design and preparation method of a cell membrane vesicle preparation loaded with mitochondria. The design method comprises the following steps: loading the mitochondria into an artificially constructed cell membrane bionic vesicle, and constructing a nano-scale vesicle preparation loaded with the mitochondria inside and a cell membrane outside; the preparation method comprises the following steps: preparing cells into a cell suspension; repeatedly extruding the cell suspension through an extrusion device provided with a filter membrane with a specific aperture; and carrying out differential centrifugation on the extruded liquid to prepare the cell membrane vesicle preparation loaded with mitochondria. The invention also discloses the vesicle preparation obtained by the method and application of the vesicle preparation in preparation of drugs for treating mitochondrial damage related diseases. According to the method, rapid and efficient loading of mitochondria in cells can be realized, and the problems of low yield of mitochondria vesicles from natural sources, limited loading efficiency and the like are solved. The prepared vesicle preparation can effectively protect the physiological function of mitochondria, delay rapid inactivation of mitochondria in the using process and achieve efficient mitochondrial transplantation of damaged cells.

The invention discloses a rare ginsenoside dammadienol synthetase and an application of the rare ginsenoside dammadienol synthetase in preparation of dammadienol. The dammadienol synthetase is identified for the first time in the research, a metabolic network of a saccharomyces cerevisiae host cell is modified through a metabolic engineering strategy, genes which do not exist in the host cell are introduced into an engineering strain for heterologous expression, the host cell is changed from nothing to existence, and the dammadienol engineering strain is constructed. In addition, through homologous modeling, key catalytic pocket searching and key enzyme gene site-directed mutagenesis modification, enzyme catalysis efficiency is improved, efficient synthesis of dammadienol is achieved, the problem that natural sources of the rare ginsenoside are insufficient is solved, green synthesis and sustainable utilization of the rare ginsenoside are promoted, and the method is suitable for industrial production. And a foundation is laid for subsequent construction of rare ginsenoside cell factories.

The invention relates to a method for extracting spermidine from a natural source. The method comprises the following steps: (1) extracting a natural source substance to obtain a crude extract; and (2) carrying out hydrophilic interaction liquid chromatography purification on the crude extract to obtain spermidine. According to the method disclosed by the invention, the extract from a natural source is efficiently and accurately separated by utilizing the hydrophilic interaction liquid chromatography, so that the high-purity spermidine with a wide source is obtained to meet the requirement on high-quality spermidine.

The invention discloses a preparation method of artemisia apiacea extracellular vesicles, which comprises the following steps: step 1, pre-treating artemisia apiacea, carrying out primary centrifugal treatment, removing large plant tissues and cell debris, carrying out secondary centrifugal treatment, filtering by a needle filter, and collecting; step 2, transferring the filtered suspension to a sucrose density gradient solution, then carrying out third centrifugal treatment, collecting the solution with the concentration of 30% in the layer, and carrying out fourth centrifugal treatment to obtain artemisia apiacea extracellular vesicles; the invention further discloses an artemisia apiacea extracellular vesicle, a pharmaceutical composition for treating cerebral apoplexy, a medicine and application of the artemisia apiacea extracellular vesicle in preparation of the medicine for treating cerebral apoplexy. The naturally-sourced vesicle delivery system provided by the invention is expected to have higher safety and lower toxic and side effects, and the enrichment concentration of the therapeutic component at the focus part of cerebral apoplexy is improved, so that the bioavailability and the treatment efficiency of the medicine are remarkably improved.

The invention discloses a bletilla striata polysaccharide hydrogel dressing and a preparation method and application thereof.The preparation method of the bletilla striata polysaccharide hydrogel dressing comprises the following steps that a carboxymethyl chitosan solution, a bletilla striata polysaccharide solution and cinnamyl aldehyde are mixed and stirred to be uniform at the temperature of 20-30 DEG C, a mixed solution system is prepared, and then standing is conducted to form gel; wherein the volume ratio of the carboxymethyl chitosan solution to the bletilla striata polysaccharide solution is (1-5): 1, and the concentration of the cinnamyl aldehyde in the mixed solution system is 0.05-5 wt%. The preparation process is simple, the raw materials are all green and natural sources, and the prepared hydrogel is good in biocompatibility, has good antibacterial, anti-inflammatory and angiogenesis promoting effects and can accelerate diabetic wound repair.

The invention discloses an anti-aging composition and a preparation method thereof, and belongs to the technical field of cosmetics, the anti-aging composition provided by the invention is prepared from the following components in parts by mass: 0.001-0.01 part of freeze-dried powder of secretin of porcine umbilical cord mesenchymal stem cells, 15-20 parts of argania spinosa leaf extract, 10-13 parts of eucheuma gelatinae extract and 1-3 parts of northeast stichopus japonicus adventitious root extract. The anti-aging composition provided by the invention has the effects of resisting aging and improving the migration rate of skin cells, and the whole anti-aging composition is mild and non-irritant; when the anti-aging composition is applied to cosmetics, the anti-aging composition can effectively resist aging, lighten wrinkles, improve skin elasticity and increase the water content of cuticles. In addition, the composition provided by the invention is natural in component source, simple in preparation method and suitable for actual production.

The invention belongs to the technical field of cosmetics, and particularly relates to a natural-source composition with a function of inhibiting multiple types of matrix metalloproteinases of skin and application of the natural-source composition. The composition is prepared from the following components: herba cistanche powder, American ginseng powder, a red-knees herb extract and a creeping rostellularia herb extract. The composition has the effects of inhibiting expression of multiple types of matrix metalloproteinases in skin immortalized keratinocytes, improving elastin synthesis and inhibiting elastase decomposition, and has no obvious side effects. The composition disclosed by the invention can be used for preparing various cosmetics, functional foods and health-care products related to skin aging resistance, and has a wide application prospect.

Provided are hair care formulations that protect the hair follicles and scalp of a subject from negative effects of hair dyeing. The hair care formulations include an antioxidant, a melanin stimulant, an anti-irritation component, a sebum modulator, a scalp repair component, and an anti-inflammatory agent. Several of these components are derived from natural sources, such as extracts from the roots of the Ononis spinosa flower, from mandarin orange fruit, and from seeds of the Schinus terebinthifolia tree. The formulations also include peptides used for melanin stimulation and scalp repair. Also provided are kits containing the scalp protection formulation, along with methods of using the scalp protection formulation. Also provided are formulations supporting hair pigmentation, hair growth, hair length, and hair health.

The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a traditional Chinese medicine composition for relieving acute kidney injury and a preparation method thereof.The traditional Chinese medicine composition comprises honeysuckle-derived exosome-like nano-vesicles (HELNVs), and the particle size distribution of the HELNVs is 68.06-164.20 nm. Firstly, the HELNVs retain various bioactive components of honeysuckle, and the HELNVs retain various bioactive components of the honeysuckle; the components can play a stronger pharmacological role through a synergistic effect; secondly, due to the nanoscale particle size (68.06-164.20 nm), the HELNVs can better penetrate through a biological barrier, and the targeting property and the bioavailability are improved; thirdly, the natural source and exosome-like structure of the HELNVs may endow the HELNVs with unique cell affinity, which is beneficial to the interaction of the HELNVs with target cells; finally, the HELNVs are used as a natural nano-carrier, so that the HELNVs have better safety.

The invention provides a genetically engineered bacterium for producing polydeoxyribonucleotide as well as a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium comprises kluyveromyces marxianus for expressing a salmon DNA polymerase I gene. According to the invention, the edible probiotic Kluyveromyces marxianus is transformed through genetic engineering, efficient, safe and controllable production of PDRN is realized, the biological activity of PDRN is equivalent to that of PDRN from a natural source, and large-scale production and application of PDRN are facilitated.

The invention relates to the technical field of food microorganisms, fermentation and seasonings, in particular to a natural fermentation flavor enhancer as well as a preparation method and application thereof. The natural fermentation flavor enhancer comprises 3-40 wt% of glutamic acid of a natural source and 0.2-25 wt% of IMP and / or GMP of a natural source. Wherein the natural fermented flavor enhancer is obtained by a method comprising fermentation by using microorganisms, and the microorganisms are selected from two or more of yeast, lactic acid bacteria, brevibacterium ammoniacum and corynebacterium glutamicum and at least comprise one of yeast or lactic acid bacteria. The natural fermented flavor enhancer is obtained through mixed fermentation of microorganisms, the flavor and taste of the flavor enhancer are improved, and astringency and peculiar smell of the product are eliminated; the natural substrate is adopted for fermentation, no additive is contained, and the requirements for greenness, safety and health are met.

A method of making monovalent and multivalent anion selective membrane. Such membrane can be used for electrodialysis (“ED”) operation and applied towards the important Cl−—SO42− separation in lithium extraction. The membrane thickness is much less than 100 μm, preferably less than 50 μm, more preferably less than 40 μm, and most preferably 20-30 μm.

The preparation method comprises the following steps: mixing a gelatin solution and sodium lignin sulfonate to obtain a mixed solution, heating the mixed solution to a set temperature, sequentially adding a polymeric monomer solution, potassium persulfate and N, N-dimethylformamide, and carrying out a reaction at a set temperature to obtain the lignin-based polymer hydrogel. Polymerizing with N, N-methylene bisacrylamide, and fully mixing to obtain the lignin-based polymer hydrogel after a gel state is formed; sodium lignin sulfonate and gelatin are adopted as bio-based raw materials, and compared with traditional lignin-based high-molecular hydrogel depending on petrochemical products, the lignin-based high-molecular hydrogel has the remarkable environment-friendly characteristic. The materials obtained from natural sources not only ensure the biodegradability of the lignin-based polymer hydrogel, but also can be naturally decomposed in soil application, so that the potential negative influence on an ecological system is greatly reduced. By using the lignin-based polymer hydrogel, secondary pollution in the environment is avoided, and the innovation potential of application of biological materials in environmental engineering and agricultural science and technology is shown.

The invention belongs to the technical field of medicines, and provides application of a natural-source compound monomer medicine composition in preparation of medicines for preventing and / or treating pancreatitis. The pharmaceutical composition comprises baicalin, paeoniflorin, geniposide, wogonin, baicalein, albiflorin, tetrahydropalmatine, rhein, wogonin, aloe-emodin, saikoside A, emodin, benzoyl paeoniflorin, chlorogenic acid, costunolide, or a pharmaceutically acceptable salt thereof. Compared with the prior art, the pharmaceutical composition disclosed by the invention has the advantages that the components of the composition are definite and are all natural sources, the safety is good, the pharmaceutical effect has a remarkable synergistic effect, the administration dosage is reduced by about 200 times under the condition that the treatment effect is better than that of a compound traditional Chinese medicine, the administration mode of the compound medicine for treating pancreatitis is innovated, and the pharmaceutical composition is suitable for clinical application. A new scheme is provided for the treatment of the disease.

The invention discloses a method for screening lipid-lowering active substances from natural extracts by improving enzyme immobilization efficiency, compared with the prior art, a polypropylene hollow fiber membrane is used as an adsorption carrier, glutaraldehyde is used as a cross-linking agent, pancreatic lipase is covalently cross-linked to the polypropylene hollow fiber membrane, and the lipid-lowering active substances are screened from the natural extracts. The enzyme loading amount, the repeated utilization rate and the storage stability of the polypropylene hollow fiber membrane are obviously improved; the enzyme immobilization efficiency and the enzyme activity are improved; furthermore, liquid chromatography-quadrupole time-of-flight mass spectrometry and a molecular network analysis technology are combined to screen 31 active ligand structures from the wild chrysanthemum flower extract with numerous and jumbled components, so that the method can be popularized and used for efficiently screening active ligands in natural products with complex components; the material basis of the lipid-lowering effect of the natural-source active ingredients can be deeply illuminated, and the active ligand has certain lipid-lowering potential and can be used as a pilot chemical of lipid-lowering drugs.

The invention discloses a natural plant-based antibacterial sanitary napkin and a preparation method thereof, and relates to the technical field of antibacterial sanitary napks.The natural plant-based antibacterial sanitary napkin comprises a surface layer, a bottom layer and an absorption core located between the surface layer and the bottom layer, and the absorption core comprises modified plant antibacterial fibers of a skin-core structure; the modified plant antibacterial fibers are prepared from matrix fibers and modified finishing liquid components through an in-situ mineralization reaction, and the modified plant antibacterial fibers are prepared from the following raw materials in parts by weight: 100 parts of matrix fibers; and 0.06-8 parts of a modified finishing liquid component. A stable metal-polyphenol coordination network is constructed on the surface of the fiber in situ, efficient and lasting antibacterial and odor removal functions are achieved, the problems that a traditional antibacterial agent is prone to loss and poor in odor removal capacity are solved, meanwhile, natural-source raw materials are adopted, excellent biological safety and hydrophilic air permeability are achieved, and the preparation method is suitable for industrial production. And dryness, comfort and safety protection in the using process are guaranteed.

The invention belongs to the technical field of biological small molecule active peptides, and more specifically relates to a uric acid reducing active peptide, a preparation method and an application thereof. The uric acid reducing activity LQKW has remarkable XOD inhibitory activity, IC50 is 2.70 mg / mL, and the IC50 is superior to that of many known natural source XOD inhibitory peptides; good gastrointestinal digestion stability is shown, and the activity retention rate after in-vitro simulated gastrointestinal digestion is (61.84 + / -0.82)%. According to the invention, a specific binding mechanism of uric acid reduction activity and XOD active sites (Ile648, Phe649 and the like) is clarified through molecular docking, and a basis is provided for rational design based on a structure. According to the invention, the method for efficiently preparing the active peptide fragment from the white spirit vinasse is established, and the high-valued utilization of the wine brewing byproducts is realized. The uric acid reducing activity LQKW provided by the invention can be prepared through solid-phase synthesis, the process is mature, the quality is controllable, and a foundation is laid for industrialization. In addition, as the XOD inhibitor of a natural source, the uric acid reducing activity LQKW has good safety.

The invention provides a radix asparagi homogeneous acidic polysaccharide. The radix asparagi homogeneous acidic polysaccharide contains pectic polysaccharide which is shown as a formula 1, takes rhamnogalacturonidan I (RG-I) as a core and has a homopolygalacturonic acid (HG) structural domain. The invention further provides a preparation method and application of the radix asparagi homogeneous acidic polysaccharide. The asparagi pectin polysaccharide with a new structure is found for the first time, and the asparagi pectin polysaccharide has the effect on acute lung injury. The acidic polysaccharide disclosed by the invention has the advantage of anti-ALI activity, realizes synergistic protection of intestine-lung double target regions, has anti-inflammatory and anti-oxidation double activities, is natural in source and high in biological safety, and is suitable for long-term adjuvant therapy of acute lung injury.

The present disclosure generally relates to the utilization of a fine mineral matter in the process of upgrading the liquid products obtained by thermolysis or pyrolysis of solid plastic waste or biomass or from cracking, coking or visbreaking of petroleum feedstocks. More particularly, the present disclosure is directed to a process of stabilization of the free-radical intermediates formed during thermal or catalytic cracking of hydrocarbon feedstocks including plastic waste and on a process of catalytic in-situ heavy oil upgrading. The fine mineral matter may be derived from natural sources or from synthetic sources.

The invention discloses a natural product composition and application thereof in reducing uric acid and blood sugar. The composition is prepared from luteolin, verbascoside and curcumin according to the weight ratio of 1: 3: 2. According to the invention, when luteolin, verbascoside and curcumin are combined according to a weight ratio of 1: 3: 2, the inhibitory activity on xanthine oxidase (XO) and alpha-glycosidase is the strongest, and the inhibitory activity is obviously superior to the inhibitory activity on xanthine oxidase (XO) and alpha-glycosidase when the luteolin, verbascoside and curcumin are independently used, so that the composition has a synergistic effect on reducing the level of uric acid and regulating blood sugar. The discovery provides a theoretical basis and a formula basis for developing novel natural-source functional products for reducing uric acid and reducing blood sugar, and has important application prospects and development values.

Modular multifunctional system, characterized by: a) at least one natural support matrix (module A), b) at least one bioactive component of natural origin (Module B), c) at least one metallic functional element (module C), d) optional synergy-enhancing additions (module D), wherein components b) and c) interact synergistically and together generate functionalities that go beyond the sum of the individual effects.

The application discloses a 100% natural source reverse micelle essential oil and a preparation method thereof, and the preparation raw materials comprise 0.6-13 parts of emulsifiers and 20-100 parts of natural source oils in terms of mass fraction. The combination of PCA glyceryl oleate and polyglycerol nonionic surfactants has a mass ratio of (0.5-0.8):(2-2.5), and the combination of polyglyceryl-2 isostearate and polyglyceryl-4 oleate has a mass ratio of (1-1.5):(0.8-1), so that the reverse micelle essential oil can be constructed, the essential oil has the advantages that the essential oil is skin-friendly and non-greasy, can be compatible with hydrophilic active ingredients and oleophilic active ingredients, and can effectively moisturize and nourish the skin.

The invention discloses a natural-source instant wrinkle-removing composition and application thereof, a cosmetic and a preparation method of the cosmetic. The natural-source instant wrinkle-removing composition is prepared from the following components in parts by weight: 0.1 to 6 parts of apple extract, 1 to 5 parts of peach resin extract and 0.01 to 0.5 part of hydroxyproline. The components in the composition are all natural products, and the composition with a good instant wrinkle removing effect can be obtained by matching the components. Meanwhile, compared with an existing wrinkle removing mode, when the natural-source instant wrinkle removing composition is used, the burden of skin can be effectively reduced on the basis that a good wrinkle removing effect is achieved, and the natural-source instant wrinkle removing composition is more friendly to the skin.

The invention relates to a skin moistening composition for a mask and a preparation method thereof, and belongs to the technical field of skin care products, the skin moistening composition is composed of sodium polyglutamate, saccharide isomeride, prunus amygdalus dulcis oil, cajeput leaf oil, an auxiliary natural extract, a permeation enhancing component, a dispersing component, a thickening component, an antiseptic component and deionized water, the auxiliary natural extract comprises a dracocephalum dracocephalum extract and a roselle extract, the permeation enhancing component is at least one of laurocapram and isopropyl myristate, the dispersing component comprises polyglycerol-10 myristate and polyglycerol-6 octoate, the thickening component comprises carbomer and hydroxyethyl cellulose, and the stabilizing component comprises a stabilizing component and a stabilizing component. According to the skin moistening composition, the preservative components comprise sodium ethylparaben and sodium methyl paraben, although the active components of the skin moistening composition are all natural source components, the skin moistening performance is still excellent, the skin moistening and moistening effects are excellent, and the skin care advantages of mildness and strong effect are shown.

The invention relates to a process for preparing a mixture of linear alkanes of natural origin, preferably of plant origin, comprising the steps of: Metathesis of at least one terminal linear olefin, comprising between 5 and 24 carbon atoms, derived from at least one fatty acid or fatty acid ester of natural origin, preferably of plant origin, in the presence of a non-isomerizing olefin metathesis catalyst, whereby a first mixture of internal olefins (I) is obtained; Isomerizing metathesis of the mixture of internal olefins (I) obtained in step i) in the presence of a catalyst or a mixture of catalysts, whereby a second mixture of internal olefins (II) is obtained, and Hydrogenation of the mixture of internal olefins (II) in the presence of a catalyst, whereby a mixture (III) of linear alkanes of natural origin is obtained.

This invention relates to a bioactivator based on L-proline structural modification, its synthesis method, and its application, belonging to the field of agricultural chemistry. The activator employs a biomimetic molecular design strategy, prepared through the splicing of active structural units. L-proline's unique natural source advantage and the important active unit fragment N,N-diethylethanolamine from DA-6 undergo an esterification reaction to yield 2-(diethylamino)ethyl-L-proline ester. Bioactivity experiments were conducted on wheat and soybean, respectively. The results showed that this compound can promote plant growth at low concentrations, exhibiting significantly better growth effects than the blank control in terms of germination rate, root length, and stem length. Furthermore, the preparation process of this invention is simple, low-cost, and easily scalable for industrial production, and it possesses good environmental compatibility. Its unique natural source advantage provides a new option for activator development and has significant potential for agricultural development.

A packaging material comprising a base layer and a film layer extending along the base layer. The film layer comprises at least a biopolymer and one or more naturally sourced additives.