1718results about "Tetrapeptide ingredients" patented technology

The invention discloses preparation and application of antioxidative, anti-inflammatory and hypoglycemic lactoferrin peptide, and belongs to the field of food science and nutriology. According to the invention, lactoferrin is used as a raw material, hydrolysis is carried out by using a composite enzymatic hydrolysis method, and the molecular weight is obtained by ultrafiltration; the preparation method comprises the following steps: preparing a component of antioxidant, anti-inflammatory and hypoglycemic peptide from the active peptide YLSW (Tyr-Leu-Ser-Trp), carrying out freeze drying on the component to obtain peptide powder rich in antioxidant, anti-inflammatory and hypoglycemic peptides, identifying peptide fragments contained in the peptide powder by utilizing an LC-MS / MS technology, and screening out the active peptide YLSW (Tyr-Leu-Ser-Trp) with antioxidant, anti-inflammatory and hypoglycemic triple functions. The active peptide is added into the product to obtain the antioxidant, anti-inflammatory and hypoglycemic functional product.

The invention discloses application of an anti-CD20 antibody drug conjugate or a drug combination thereof in preparation of a drug for treating diffuse large B-cell lymphoma. The drug combination comprises the anti-CD20 antibody drug conjugate, gemcitabine and oxaliplatin, the anti-CD20 antibody drug conjugate or the drug combination therapy containing the anti-CD20 antibody drug conjugate has a better curative effect compared with the existing clinical second-line standard therapy.

The invention relates to a Trop2-targeting antibody coupling drug as well as a preparation method and application thereof, in particular to a nano antibody specifically bound with Trop2 or an antigen binding fragment thereof, a polypeptide construct thereof, an antibody coupling drug, a composition and medical application. The antibody coupling drug has excellent affinity to Trop2 protein, shows good targeting and inhibitory activity to Trop2 positive tumor cells, and is good in safety.

The invention discloses a tetrapeptide LILP with uric acid reducing activity and application thereof, and belongs to the technical field of biological medicine. The amino acid sequence of the tetrapeptide LILP is Leuu-Ile-Leu-Pro, and the amino acid sequence of the tetrapeptide LILP is shown in the specification. The invention discloses that the tetrapeptide LILP has uric acid reducing activity for the first time, and uric acid generation can be reduced by reducing the activity of xanthine oxidase in the liver; uric acid discharge can be increased by reducing kidney uric acid reabsorption. The invention provides a multi-target active substance for reducing uric acid, which can be applied to preparation of medicines for preventing or treating hyperuricemia or preparation of health-care foods for assisting in reducing the level of uric acid, and has a good application prospect.

The invention discloses a camel blood polypeptide and application thereof in preparation of an alpha-glucosidase inhibitor, a series of polypeptide products are separated from camel blood, and it is found that the camel blood polypeptide with the amino acid sequence being YFKI, DPPTMEL, DKPFGPDL or SEPSGTAFG has the remarkable alpha-glucosidase inhibitory activity; the compound can be applied to preparation of drugs for treating diseases caused by abnormal activity of alpha-glucosidase.

The invention discloses a wheat peptide with a hypoglycemic effect as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The invention provides a bioactive peptide LPQ or LPQF with a hypoglycemic effect, and animal model experiments prove that the bioactive peptide can effectively reduce the blood sugar level and improve related metabolic indexes, and has no obvious side effects. The invention also provides a wheat peptide rich in oligopeptide containing the peptide fragment LPQ, and animal model experiments prove that the wheat peptide has a remarkable effect in the aspect of reducing blood sugar. The invention provides a safe and effective alternative scheme for development of diabetes treatment drugs, and has good market prospects and application potential.

The disclosure relates to methods, compositions, and combinations for detoxification in a patient comprising performing therapeutic plasma exchange (TPE) on the patient. The disclosure also relates to methods, compositions, and combinations for removing toxins in a patient as a result of chronic exposure to the toxins, comprising performing one or more TPE sessions on the patient and administering an oral composition to the patient. The methods, compositions, and combinations of the disclosure may also be used to replenish biomarkers indicative of a healthy state of the body in order to improve immune system function or patient longevity, and / or reduce oxidative stress or inflammation. The methods, compositions, and combinations of the disclosure may also be used to reduce the level of atherosclerosis, improve cognitive function, prevent early cancer development, promote immune health, and / or treat health conditions or diseases such as autoimmune diseases, cancers, Alzheimer's disease, Parkinson's disease, chronic fatigue syndromes, or fibromyalgia. Additionally, the methods, compositions, and combinations of the disclosure may comprise an intravenous supplementation, wherein each TPE session is followed by administration to the patient of an IV composition. The disclosure further relates to oral compositions and IV compositions for use in the methods, compositions, and combinations of the disclosure.

The invention provides a hypoglycemic peptide which is prepared by taking bovine whey protein as a raw material and is stable in gastrointestinal digestion and absorption. In an in-vitro cell experiment, the peptide fragment can improve the uptake of insulin-resistant HepG2 hepatocytes and synthesize glycogen by using glucose, can promote intestinal endocrine cells NCI-H716 to secrete GLP-1, has a certain synergistic effect, and can further play the effects of promoting GLP-1 secretion and activating a GLP-1 receptor through combined use. An insulin resistance mouse model is constructed by utilizing high-fat diet, and the peptide fragment is adopted for intervention, so that the hypoglycemic peptide is further proved to have obvious effects on losing weight, reducing lipid accumulation and assisting in reducing blood fat, and meanwhile, the hypoglycemic peptide can be used for improving insulin resistance related symptoms. The milk peptide provided by the invention has gastrointestinal digestion stability and hypoglycemic activity, can be used as a functional milk base material for developing food, medicines or health care products with the effect of regulating blood sugar, and is wide in application prospect.

The invention provides cubilose peptide derived from cubilose and capable of promoting silk polyprotein and inhibiting expression of inflammatory factors and application of the cubilose peptide. The amino acid sequence of the peptide is as shown in at least one of SEQ ID NO: 1-5. The peptide disclosed by the invention is derived from cubilose, has the performance advantages of being small in molecular weight, promoting the expression of cell filament protein, inhibiting the expression of cell inflammatory factors and reducing the level of reactive oxygen free radicals of cells, has the biological activity of moisturizing, resisting inflammation and resisting oxidation, can be used for preparing medicines, daily chemical products, moisturizing products, anti-oxidation products or anti-inflammatory products, and is wide in application prospect.

The invention discloses tetrapeptide with a hypoglycemic effect as well as preparation and application thereof, and belongs to the technical field of biological medicines. The amino acid sequence of the tetrapeptide LLRP is Leu-Leu-Arg-Pro, and the tetrapeptide LLRP has DPP-IV inhibitory activity, can effectively reduce the blood sugar level and has no obvious side effect, so that the tetrapeptide LLRP can be applied to preparation of drugs or food for reducing blood sugar. The invention further provides polypeptide containing the peptide fragment LLRP and wheat peptide rich in the polypeptide, tetrapeptide LLRP with the effect of reducing blood sugar is generated after digestion of gastrointestinal tracts, and the tetrapeptide LLRP is applied to preparation of drugs or food for reducing blood sugar. The invention provides a new scheme and theoretical basis for treatment of diabetes, and has good market prospect and application potential.

A group of polypeptides, which can be used as a tyrosinase inhibitor. The polypeptides have the effects of inhibiting tyrosinase activity, reducing melanin secretion, resisting oxidation, skin-whitening, spot-removing, etc. The polypeptides can be widely used in antioxidant drugs and in the preparation of skin-whitening cosmetics for resisting melanogenesis and lightening spots as a new raw material, and has good application scenarios.

The invention relates to the technical field of biology, in particular to an ACE inhibitory peptide, a preparation method and application, the ACE inhibitory peptide has the amino acid sequence of Trp-Pro-Phe (WPF) or Phe-Pro-Trp (FPW) or Phe-Gly-Trp (FGW) or Leu-Pro-Val-Pro-Ala-Phe-Asn (LPVPAFN) or Leu-Trp-Trp-Leu (LWWL), the ACE inhibitory peptide WPF, FPW, FGW, LPVPAFN and LWWL have outstanding antihypertensive activity, the IC50 values of the WPF, FPW, FGW, LPVPAFN and LWWL are 0.264 mg / mL, 0.800 mg / mL, 0.106 mg A variety of aquatic product peptide powder sources are separated and purified by an affinity ultrafiltration screening method, an ultrafiltrate component is selected for amino acid sequence identification, and a new amino acid sequence with ACE inhibitory activity is screened out; the ACE inhibitory peptides WFP, FPW, FGW, LPVPAFN and LWWL prepared by the invention have outstanding antihypertensive activity.

The invention discloses a fish by-product active peptide, a preparation and application, and belongs to the technical field of biological active peptides. The amino acid sequences of the fish by-product active peptide are shown as SEQ ID NO: 1 to SEQ ID NO: 3. Both the single peptide fragment and the compound peptide fragment of the fish by-product active peptide can obviously inhibit NO secretion of a lipopolysaccharide (LPS)-induced macrophage (RAW264.7) inflammation model, so that the fish by-product active peptide has a relatively good application prospect in preparation of a product with an anti-inflammatory effect; and a foundation is laid for high-added-value utilization of salmon byproducts and promotion of research, development and application of functional active peptides.

The invention discloses an oligopeptide from undaria pinnatifida and application thereof. The amino acid sequence of the oligopeptide is YRKD, and the molecular weight is 580.63 g / mol. The polypeptide is identified from undaria pinnatifida through a high performance liquid chromatography technology for the first time, and an unreported oligopeptide capable of inhibiting tyrosinase and cooperating with vitamin C to synergistically inhibit tyrosinase is screened out. The oligopeptide derived from undaria pinnatifida is synthesized by a solid phase method, has the characteristics of safety, no toxicity, good water solubility and good stability, and is combined with vitamin C to generate a synergistic effect and synergistically increase the inhibition effect on tyrosinase, so that the oligopeptide can achieve a better tyrosinase inhibition effect at a lower and more stable concentration; the use concentration of the oligopeptide can be greatly reduced, the stability is enhanced, and the oligopeptide can be widely applied to preparation of cosmetics, medicines, health-care products or food additives, and has important economic value and social significance.

Compositions and methods for treating diseases associated with dysregulation of miR-21, particularly cancer, are disclosed.

The present invention relates to the fields of medicine and cosmetology, and discloses a cell active peptide composition that promotes the regeneration of multi-type collagen structural proteins, its preparation method, and its application in medicine and cosmetology. The composition uses raw materials such as tripeptide-10 citrulline, hexapeptide-9, hydrolyzed sodium hyaluronate, γ-aminobutyric acid, decarboxylated carnosine hydrochloride, acetyl tetrapeptide-3, acetyl tetrapeptide-11, palmitoyl dipeptide-7, and palmitoyl pentapeptide-4. The composition can promote the coordinated expression of skin type I, type III, type IV, type VII, type XVII collagen, fibronectin, elastin, and glycosaminoglycans; prevent collagen cross-linking, maintain triple helix structure, and inhibit collagen degradation. Applied to dermatology and cosmetology, it can accelerate the regeneration of structural proteins such as skin collagen, repair skin damage, reduce wrinkles such as crow's feet, tear groove lines, nasolabial folds, and under-eye lines, solve the skin aging problem caused by the loss of structural proteins such as collagen, and improve skin elasticity and firmness.

The invention discloses a polypeptide as well as preparation and application thereof, and belongs to the technical field of active peptides. According to the present invention, three active peptides are prepared from walnut meal protein, the amino acid sequences of the three polypeptides are respectively Leuu-Pro-Gln-Phe (LPQF), Leuu-Pro-Ser-Phe (LPSF) or Vla-Pro-Phe-Pro (VPFP), and the detection results show that the three polypeptides have inhibition activity on dipeptidyl peptidase-IV (DPP-IV), such that the three polypeptides can be used for preparing the blood sugar reducing drug.

The invention discloses a horse milk-derived small molecule peptide, the amino acid sequence of which is Met-Ser-Pro-Phe (MSPF), and the molecular weight of which is 480.571 Da. The small molecule peptide disclosed by the invention has the activity of inhibiting pancreatic lipase (PL), the inhibition rate on the pancreatic lipase is 61.44%, and the accumulation of the content of triglyceride (TG) in oleic acid-induced human liver cancer (HepG2) cells can be reduced, so that the small molecule peptide can be used for inhibiting the pancreatic lipase (PL). The small molecule peptide can be applied to preparation of preparations for treating or assisting in treating hyperlipidemia and obese people, is simple to prepare, and is suitable for industrial production and market popularization and application.

The invention discloses a blood protein precise active peptide capable of tonifying qi and blood, improving microcirculation and promoting vasodilation and a preparation method and application thereof, and belongs to the technical field of blood protein peptide. The blood protein precision active peptide comprises at least one of LGFP, IGFP, FPHFN, LFL, LFI, FESF, ILF, LIF, FLL, FIL, FII or FPHFD. The prepared blood protein precision active peptide has multiple biological activities: an antihypertensive effect is achieved by inhibiting ACE and vasoconstriction substances; vasodilation is promoted by activating a PI3K / AKT / eNOS signal channel; a zebra fish model proves that the traditional Chinese medicine composition has the effects of remarkably improving microcirculation and tonifying qi and blood. The preparation method is low in equipment requirement, simple in process, easy to operate and convenient for industrial large-scale production. The method enriches a bioactive peptide library, and is of great significance in promoting technical progress and industrial upgrading in the field of hemoglobulin peptides.

The invention provides a composition with a function of stabilizing skin enzyme activity as well as a preparation method and application of the composition, and the composition is prepared from the following components in parts by mass: 0.001 to 0.01 part of superoxide dismutase, 0.001 to 0.05 part of nicotinamide adenine dinucleotide, 0.11 to 12 parts of a polypeptide compound a and 0.005 to 0.5 part of a polypeptide compound b. The invention also provides a preparation method of the composition and application of the composition in preparation of skin care products for improving skin enzyme activity, repairing skin and resisting aging. The composition can be prepared into emulsion, solution, paste, gel or spray. The composition can effectively improve the enzyme activity of skin, improve the texture and appearance of the skin and repair the skin, and is beneficial to delaying the aging of the skin at the same time.

The invention discloses a grape seed source anti-inflammatory peptide, and belongs to the technical field of biological medicine. The amino acid sequence of the anti-inflammatory peptide is selected from any one of WGF, SHFGF, EGPFF, WAPR, SFYRAF, HFAFL, PGRF or FDSF. A BPS-induced RAW 264.7 mouse mononuclear macrophage inflammation model is established, and the eight synthetic peptides are found to significantly reduce the content level of NO in inflammatory cells and have an inhibition effect on secretion of inflammatory factors TNF-alpha and IL-6. A qRT-PCR (quantitative reverse transcription-polymerase chain reaction) experiment shows that the synthetic peptide can inhibit expression of mRNA (messenger Ribonucleic Acid) of p65, Tnf alpha, Il6, Il1b and Nos2, and can play an anti-inflammatory role by regulating and controlling an NF-kappa B signal channel. According to the method, the high-activity anti-inflammatory peptide is screened from the wine brewing byproduct grape seeds for the first time, and green and high-value utilization of the grape processing byproduct is realized.

The invention discloses a cubilose peptide with ACE inhibitory activity and a preparation method and application thereof, the cubilose peptide with the ACE inhibitory activity is prepared through enzymolysis, meanwhile, LC-MS / MS analysis is performed on peptides in enzymatic hydrolysate, a peptide fragment with potential ACE inhibitory activity is screened out in combination with a bioinformatics means, and the obtained peptide fragment has high ACE inhibitory activity through synthesis verification. The bird's nest ACE inhibitory peptide disclosed by the invention has a good application prospect in functional foods or health-care products and food-borne medicines.

The invention provides a spirulina-derived ACE (angiotensin converting enzyme) inhibitory peptide as well as a preparation method and application thereof, and belongs to the technical field of marine bioactive peptides. Spirulina phycobiliprotein is used for preparing the ACE inhibitory peptide of the spirulina source, five kinds of small molecule polypeptides including YATY, RYVTY, YVTY, KAYF and SPSWY are obtained through co-screening, the five kinds of polypeptides all have good ACE inhibitory activity, and the RYVTY activity is the highest. The spirulina-derived polypeptide prepared by the preparation method disclosed by the invention has high hydrolysis degree and high ACE inhibitory activity, can be applied to the fields of food, health care products, medicines and the like, and has a wide application prospect.

The invention belongs to the technical field of active peptides, and particularly relates to an active peptide capable of improving memory, a brain peptide enzymolysis extract and a preparation method and application of the brain peptide enzymolysis extract. The amino acid sequence of the active peptide is shown as SEQ ID NO.1 Ala-Ala-Val-Pro, and the active peptide can improve sleep and improve memory; on the other hand, the invention provides a preparation method of the brain peptide enzymolysis extract. The preparation process comprises the steps of raw material pretreatment and grinding, microwave treatment and centrifugal separation, protein precipitation and enzymolysis, microwave-assisted enzymolysis and purification and nanofiltration membrane filtration. Pepsase is added in the microwave treatment, and the temperature is not more than 50 DEG C in the enzymolysis process, so that the small molecule peptide keeps relatively high activity; furthermore, carbon dioxide gas is introduced in the microwave treatment for aeration, and the aeration pressure and the pH value are optimized, so that the activity and the yield of the brain peptide enzymolysis extract are further improved.

The invention discloses a novel polypeptide, a polypeptide derivative and application thereof, and belongs to the technical field of biological medicine. The invention specifically relates to a specific sequence, a discovery process, a specific anti-tumor type, a long-acting modification mode and application of the novel polypeptide. The tumors comprise one or more of brain glioma, neuroblastoma, head and neck cancer, esophagus cancer, thyroid cancer, lung cancer, liver cancer, kidney cancer, breast cancer, cervical cancer, uterine cancer, ovarian cancer, colon cancer, small intestinal cancer, ileocecum cancer, stomach cancer, bladder cancer, pancreatic cancer, prostate cancer, bile duct cancer, melanoma, sarcoma, myeloma, lymphoma and leukemia; the compound has the specific effect of inhibiting proliferation and / or metastasis of tumor cells, is wide in treatment spectrum and has important treatment value in various tumors.

The invention discloses a long-acting anti-aging composition capable of quickly removing dark circles and application of the composition. The composition is prepared from the following components: beta-nicotinamide mononucleotide, glutathione, acetyl hexapeptide-8, acetyl tetrapeptide-5, snake venom-like peptide, arginine / lysine polypeptide, tocopheryl acetate and ergothioneine. According to the present invention, multiple functions are adopted, such that the effects of dark circle removing, repair, elasticity recovery, aging resistance and the like are provided so as to block the generation of dark circle, and the normal function of skin is ensured. The composition disclosed by the invention has the effect of quickly removing dark circles, and when the eye cream contains the composition, the eye cream can effectively remove the dark circles and has an obvious and quick effect within 15 minutes; after being used for a long time, the eye mask can remove dark circles and play a good control role.

The invention discloses a bioactive peptide with antioxidant and hypoglycemic effects, a preparation and application thereof, and belongs to the technical field of bioactive peptides. The bioactive peptide provided by the invention is as shown in at least one of SEQ ID NO: 1 to SEQ ID NO: 4. Degreased shiny-leaved yellowhorn meal is used as a raw material, after high-temperature heat treatment and ultrahigh-pressure pretreatment are performed, the bioactive peptide with antioxidant and hypoglycemic effects is separated through enzymolysis, ultrafiltration and a liquid chromatography separation technology in combination with a bioinformatics technology, and components and sequences of the bioactive peptide are preliminarily identified through a liquid chromatography-mass spectrometry technology. And carrying out chemical synthesis according to the identified peptide fragment sequence to obtain four bioactive peptides with antioxidant and hypoglycemic effects. Through detection, the peptide fragments and the compound peptide fragments thereof have good alpha-glucosidase and alpha-amylase inhibitory activity, and have good application prospects in preparation of products with antioxidant and hypoglycemic effects.

The invention relates to combined application of an antibody drug conjugate and an immune checkpoint inhibitor, in particular to application of single drug or combined use of the antibody drug conjugate in preparation of drugs for preventing and / or treating cancers. Specifically, the invention provides an application of the antibody drug conjugate or pharmaceutically acceptable salt, metabolite or solvate single drug thereof or a combined immune checkpoint inhibitor in preparation of drugs for preventing and / or treating cancers.

The invention discloses a preparation method of polypeptide with an anti-inflammatory effect. The preparation method comprises the following steps: S1, swelling resin; s2, removing Fmoc protecting groups of the resin; s3, washing the resin, and draining the cleaned resin to ensure that the resin reaches the cleanliness required by the next-step reaction; s4, amino acid coupling; s5, removing an Fmoc protecting group in the amino acid; s6, polypeptide cutting: preparing a cutting reagent, after the last amino acid is synthesized and the Fmoc group is removed, cleaning the resin, adding the cutting reagent, putting a synthesis tube into a shaking table, oscillating, cutting, collecting a cutting liquid into a centrifugal tube, and drying by blowing; s7, polypeptide purification; and S8, polypeptide freeze-drying: performing freeze-drying treatment on the purified polypeptide solution through a low-temperature freeze-drying machine to finally obtain solid powder of the target polypeptide. The anti-inflammatory effect can be improved, adverse reactions are reduced, toxic and side effects on the body in the anti-inflammatory process are reduced or eliminated, and the varieties of anti-inflammatory polypeptides are enriched.

To provide modified or polymer conjugated MetAP2 inhibitors.SOLUTION: The present invention relates to methods of preventing, inducing, causing or increasing weight loss, treating obesity and / or treating metabolic syndrome utilizing the modified or polymer conjugated MetAP2 inhibitors. The present invention also relates to methods of improving insulin sensitivity and glycemic control, reducing insulin levels and / or improving leptin sensitivity utilizing the modified or polymer conjugated MetAP2 inhibitors.SELECTED DRAWING: Figure 1