27 results about "Cellular membrane" patented technology

The present application belongs to the technical field of daily chemical preparation, and particularly relates to a gynecological gel for inhibiting bacteria, regulating microecological balance and tightening vagina and a preparation method thereof. The gynecological gel comprises the following raw materials in mass fractions: 0.5-1 parts of carbomer, 0.04-0.09 parts of sodium hydroxide, 1-3 parts of collagen, 0.01-0.06 parts of sodium hyaluronate, 0.5-1.5 parts of isomaltooligosaccharide, 2-5 parts of propylene glycol, 0.05-0.3 parts of hydroxybenzoic acid methyl ester, 0.02-0.15 parts of hydroxyphenyl ethyl ester, 0.1-1.5 parts of hydrogenated lecithin, 1-6 parts of 1,3-butanediol, 3-5 parts of glycerol, 0.5-1 part of xanthan gum and 70-90 parts of water. The present application uses buckwheat filtrate to prepare collagen, and the active ingredients in the buckwheat filtrate can penetrate the cell membrane of pathogenic bacteria to play an antibacterial role; the collagen can repair the vaginal mucosa barrier and reduce the adhesion and invasion of pathogenic bacteria; therefore, after being compounded with the raw materials such as carbomer, sodium hydroxide, sodium hyaluronate, isomaltooligosaccharide and propylene glycol, the multiple components can synergistically promote the synthesis and repair of collagen fibers and improve the elasticity of tissues.

This invention relates to the field of biomedical technology, specifically to a microfluidic channel, a microfluidic chip, and their applications for squeezing cells. The microfluidic channel is composed of several continuously arranged cell squeezing units. Each cell squeezing unit consists of a main channel and a squeezing region. The main channel is composed of connecting sections and curved sections. The squeezing region includes a first squeezing section, a squeezing slit, and a second squeezing section. The width of the first squeezing section gradually decreases from the main channel, reaching its minimum at the squeezing slit, and then gradually increases towards the second squeezing section until it matches the width of the main channel. The squeezing slit is rectangular, with a width of 4-10 μm and a length of 20-60 μm. The microfluidic channel provided by this invention applies relatively uniform and dispersed mechanical stress to the cell membrane, reducing cell breakage and mortality rates, and allowing cells to restore their integrity through the cell membrane's self-repair mechanism after squeezing.

This invention relates to the fields of brachytherapy and radiosensitization, specifically providing a... 125 I-nano implantable particles, their preparation methods, and applications. 125 The 1-nanometer implanted particles have a core-shell structure, consisting of a particle core and particles adsorbed on the outer surface of the particle core. 125 I and the particles encapsulated in the nucleus and 125 The outer shell of the I-type photosensitizer is a covalent organic framework. This invention... 125 The core of the I-nanometer implanted particle is used to enhance the energy deposition of gamma rays and X-rays, generating more hydroxyl radicals; the shell containing a type I photosensitizer is used to absorb the energy of Auger electrons and internal conversion electrons and excite the photosensitizer to generate singlet oxygen, effectively improving the response to... 125 The efficiency of I decay energy utilization. 125 I-nano implanted particles, combined with internal radiation therapy and radiodynamic therapy, destroy tumor cell DNA and cell membranes, enhance tumor killing and induce immunogenic cell death, thereby activating anti-tumor immunity and inducing remote effects.

A computational system and method for digital modeling of cardiac cell membrane electrophysiology is disclosed. The system generates a digital model representing the cell membrane as a dielectric lipid bilayer comprising a capacitor element and a resistor element, where the resistor element is emulated by a plurality of ion channels. The system calculates cell membrane potential based on intracellular and extracellular concentrations of key ions, such as Na+, K+, Ca2+, and Cl-, using the Goldman-Hodgkin-Katz equation. Generation of cellular membrane action potentials is simulated by modeling the opening and closing of voltage-gated ion channels in response to triggering events. The model provides dynamic visualizations of the distinct phases of the action potential, including depolarization and repolarization, offering a high-fidelity tool for research and for enhancing the realism of training simulations in electrophysiology procedures.

This invention provides a method, program, and apparatus for more easily designing delivery carriers suitable for target cells. [Solution] A method for predicting delivery carrier composition comprises the steps of preparing a delivery carrier prediction tool constructed by machine learning using a delivery carrier composition dataset of multiple types of delivery carriers exhibiting different component compositions, a non-target cell membrane composition dataset of multiple types of cells other than target cells relating to cell membrane lipid composition, and a non-target cell delivery amount dataset of multiple types of delivery carriers relating to the amount of delivery substances delivered to multiple types of cells other than target cells as training datasets, and using the delivery carrier prediction tool to obtain a delivery carrier composition that satisfies a target value based on the cell membrane lipid composition of target cells.

The application discloses a design and application of a temperature-sensitive solid-liquid phase change peptide-based condensate probe, and belongs to the field of biomedical technologies. The peptide-based probe can form a condensate droplet through solid-liquid phase change near body temperature, and forms a gel at room temperature, can penetrate a cell membrane and produce fluorescence when combined with RNA.

The application relates to the biotechnology field and discloses a drought stress related alfalfa gene and application thereof, and the MsCAS15 gene is cloned from alfalfa, the nucleotide sequence of which is shown as SEQ ID NO. 1, and the encoded dehydrin is shown as SEQ ID NO. 2. The protein is rich in glycine and lysine and has the functions of protecting cell membranes and biological macromolecules. The application obtains a transgenic plant overexpressing the MsCAS15 by constructing a recombinant expression vector containing the gene and using an agrobacterium-mediated method. The experimental results show that overexpression of the gene enhances the drought resistance of the plant, and the specific performance is that the seed germination rate of the transgenic plant is higher than that of the wild type under osmotic stress, and under the natural drought condition of soil, the wilting degree of the transgenic plant leaves is reduced, and the growth state is better than that of the wild type. The application provides an effective gene resource for cultivating new drought-resistant crop varieties.

Provided is a cell stretching device that creates stress by applying stretching to the cell to trigger mechanical signaling in the cell and thus biochemical signals. The cell stretching device includes the following: four stretching motors providing bidirectional linear movement and a control unit for controlling the frequency and movement distances of said stretching motors, at least one stretching plate connected to the linear movement outputs of the stretching motors for each edge of said membrane and a plurality of pin openings provided on the stretching plates for each membrane edge and enabling said membrane to be connected to the stretching plates.

This invention discloses a three-dimensional imaging method for biological tissue samples based on CUBIC clearing antibody staining, comprising: placing mouse brain tissue in CUBIC-1 solution for degreasing and dehydration; subjecting the degreased and dehydrated mouse brain tissue to sequential cell membrane permeation and blocking treatments; incubating the blocked mouse brain tissue with diluted primary antibody GFAP and secondary antibody Alexa488; washing the mouse brain tissue after two antibody incubations; placing the washed mouse brain tissue in CUBIC-2 solution for matching, and then embedding the mouse brain tissue in resin; placing the resin-embedded sample in a buffer bath, and acquiring images using a TDI-fMOST camera. This invention achieves overall staining of large biological tissue samples using CUBIC clearing antibody staining.

This invention belongs to the interdisciplinary field of pharmaceutical formulation and gene therapy, and discloses a dsRNA targeting ALK7, its composition, and its applications. The ALK7-targeting dsRNA comprises a sense strand and an antisense strand. The antisense strand includes a complementary region to the ALK7 RNA transcript, and the complementary region contains at least 15 consecutive nucleotides differing from one of the antisense sequences listed in Tables 1-2 by 0, 1, 2, or 3 nucleotides. The nucleotide sequence of the sense strand is as shown in any one of SEQ ID NO. 6-277, and the nucleotide sequence of the antisense strand is as shown in any one of SEQ ID NO. 278-549. The dsRNA of this invention, through chemical modification, optimizes its ribose and phosphate ester structures to exhibit resistance to nuclease degradation and significantly reduces the negative charge carried by the molecule, enhancing its ability to permeate cell membranes. Furthermore, this invention can reduce fat accumulation at its source, improve insulin resistance, and reduce the risk of other metabolic diseases.

The present application relates to the technical field of biological medicine, and particularly relates to a tumor immunogenic death biomimetic nanovesicle with high membrane breaking efficiency, a preparation method and application thereof.The tumor immunogenic death biomimetic nanovesicle with high membrane breaking efficiency comprises a tumor cell membrane and a photothermal reagent; the photothermal reagent is compound A or compound B.The biomimetic nanovesicle provided by the present application has a killing rate of more than 95% on various tumor cells, can be prepared into ICMs for different tumors by flexibly matching cell membranes of different cancer types, supports individualized treatment and combined immunotherapy, and provides an innovative scheme with high efficiency, wide spectrum and low toxicity for solid tumor treatment through synergistic action of targeted delivery and photothermal-induced cell membrane destruction, and has high clinical application potential.

The application discloses a preparation method of a biomimetic membrane coated nano preparation based on regulation of in-vivo distribution of nanomedicine by fusing cell membranes and application thereof, and belongs to the field of biological medicine and nanometer delivery technology. The method fuses 4T1 cell membranes and red blood cell membranes at a certain ratio, and coats the fused membranes on the surface of nanoparticles to construct the biomimetic membrane coated nano preparation. The method can regulate the in-vivo distribution behavior of nanomedicine, so that the nanomedicine can exhibit spleen-biased distribution while maintaining certain tumor recognition capability. The preparation has the advantages of simple preparation method, good biocompatibility and strong regulation, and can be used for preparing a drug for preventing and / or treating breast cancer.

Table linen (6), in particular disposable table linen and / or textile table linen, wherein the table linen (6) is soaked and / or impregnated with an active ingredient, wherein this active ingredient has an antiviral and / or antibacterial effect, wherein the active ingredient is an antiviral active ingredient with a biocide, in particular silver chloride and / or a chemically activated saline solution and / or a silver phosphate glass and / or a chemical-free solution at the molecular level, wherein positively charged ions are used which, upon contact, damage and / or destroy the negatively charged viral, microbial cells, and / or is silver chloride and / or titanium dioxide.and / or wherein the active ingredient has an antibacterial effect to prevent body odor and / or wherein the active ingredient prevents infection of healthy human cells by harmful viruses and / or wherein the active ingredient works with a silver and vesicle technology and / or wherein the active ingredient has positively charged nanofibers and / or wherein the active ingredient is based on a sustainable, biological, non-chemical basis and / or wherein the active ingredient is based on a specially modified water-based polymer emulsion based on special polymer mixtures, the basis of which is physical and / or mechanical in nature and / or wherein the active ingredient exhibits strong antimicrobial properties after curing,which are solely attributable to hydrophobic interactions of the cured polymer emulsion with the cell membrane of microorganisms and / or wherein this antimicrobial effect is purely physical and / or mechanical in nature and is not attributable to chemical or ionic interactions and / or wherein the polymer emulsion results in a polymer coating and / or wherein the chemical and physical properties of the polymer coating are modeled on the natural structure of microorganisms and / or wherein the active ingredient is free of biocides and nanoparticles.

The application discloses a method for detecting circulating tumor cells based on cell membrane surface sialic acid expression quantity and single cell detection technology, and belongs to the field of biomedical technology. The method comprises the following steps: using a boron acid group modified probe to label cell surface sialic acid in a blood sample after erythrocyte lysis; passing the labeled sample through a phase transfer flow focusing chip to enrich and purify circulating tumor cells based on cell size; and detecting the enriched and purified cells by a single cell analysis method. The application solves the problems of high cost, long time consumption and high missed detection rate of the current circulating tumor cell detection method applied in clinical practice, and provides a method with simple operation and higher universality for circulating tumor cell detection.

The application discloses an optimized method for preparing amniotic membrane stem cell membrane pieces, relates to the technical field of tissue engineering and regenerative medicine, and significantly shortens the preparation period of the amniotic membrane stem cell membrane pieces by adding nicotinamide adenine dinucleotide (NAD+) and a precursor compound beta-nicotinamide mononucleotide (NMN) in a traditional cell membrane piece culture medium, combining with vitamin C (VC) and a mechanical scratching method. More importantly, the method can effectively reduce the surface expression amount of stem cell CD34 in the membrane pieces, from about 2.04% in a conventional method to below 0.12%, and the prepared stem cell membrane pieces have lower immunogenicity and more definite mesenchymal stem cell characteristics, so that the safety and effectiveness of the stem cell membrane pieces in clinical transplantation application are greatly improved.

This invention discloses an engineered stem cell membrane biomimetic formulation, its preparation method, and its applications. The biomimetic formulation of this invention consists of core-shell structured drug-loaded nanoparticles. The core is a lipid polymer hybrid nanoparticle loaded with a drug for reprogramming microglia metabolism; the outer shell is an engineered stem cell membrane on which ligands that specifically target microglia are highly expressed. The engineered stem cell membrane-coated biomimetic formulation provided by this invention has a simple preparation process and features high drug loading capacity, controlled release, high blood-brain barrier penetration, and precise microglia targeting capability. It can achieve synergistic therapeutic effects through microglia energy metabolism reprogramming and has high clinical research value in the treatment of neurodegenerative diseases.

In the present invention, a current related to a cell membrane formed in a well is measured appropriately by a measurement circuit. Specifically, a cell membrane current measurement device 1 comprises a plate-shaped chip 10 and a substrate 76 disposed so as to face the chip 10. A well 20 for forming a cell membrane 64 is provided in the surface 11 of the chip 10 on the opposite side from the substrate 76. An electrode 25 disposed in the well 20 is exposed on a surface 12 of the chip 10 on the substrate 76 side. The substrate 76 is provided with a measurement circuit 100 for measuring a current I related to the cell membrane 64 formed in the well 20. The substrate 76 is provided with a conduction member 70 fixed to the substrate 76 and extending linearly from the substrate 76 toward the chip 10. The electrode 25 and the measurement circuit 100 are electrically connected by bringing the conduction member 70 into contact with the electrode 25.

In some aspects, this disclosure relates to high-throughput systems and methods of using the same for intracellular delivery of one or more payloads into individual cell samples. For example, in some aspects, a high-throughput liquid processor is provided, configured to distribute mechanically disturbed cells in a form suitable for high-throughput cell assays. In some other aspects, this disclosure relates to components of the high-throughput system, such as a liquid processor and a housing comprising a filter having a plurality of pores penetrating the filter for agitating cell membranes; and the use of the high-throughput system.

The application discloses a vanadium carbide nano material capable of enhancing photothermal therapy and application thereof, and relates to the technical field of biomedical materials. The vanadium carbide nano material comprises vanadium carbide nanosheets and cell membrane vesicles. The vanadium carbide nano material is coated with V2C nanosheets through engineering of tumor cell membrane vesicles, target delivery is realized, and the photothermal therapy effect is significantly enhanced by utilizing the characteristic of vanadium elements to block the HSF1 phase separation process. The vanadium carbide nano material integrates multiple functions such as target delivery, photothermal conversion, oxidative stress induction and HSF1 phase separation inhibition, and effectively overcomes the heat tolerance of tumor cells.

This invention discloses a label-free, in-situ detection device and method for single-cell membranes, belonging to the field of mass spectrometry imaging technology. The device sequentially comprises a VUV laser generator, a spectrometer, an ionization chamber, a Time-of-Flight (TOF) mass analyzer, and a data acquisition processor. The spectrometer includes a spectroscopic system. This invention utilizes a lens focusing method, adjusting the distance between plano-convex mirrors in the spectroscopic system to split and focus the VUV laser, resulting in a minimal focused spot. The optimal lateral and longitudinal resolution (12 nm) of the device was determined by characterizing the spot size. Using this device to detect HeLa cell membranes, it was confirmed that it can accurately peel off real cell membranes, effectively improving the device's resolution, especially the longitudinal resolution, providing more accurate reference information for fields such as cancer research, cell communication, drug screening, and disease diagnosis.

A method of treatment includes modifying and / or killing cells by changing the cell membrane charging time to make the cells more susceptible to the influence of electric fields. The method may further include inserting one or more electrodes into tissue, acquiring a temperature of the tissue, delivering electrical energy to the tissue by electrical pulses, determining a rate of energy delivery of the electrical energy such that the temperature rises above a first critical temperature but remains below a second critical temperature, and delivering the electrical energy until a specific dose is achieved.

The application provides a nerve activator for regulating opening of a MSC and application, and belongs to the technical field of nerve activation.The nerve activator is composed of an optical tweezer system and microparticles.The application utilizes the optical tweezer system to convert dynamic optical force into vibrating mechanical force through microparticle medium, and then precisely acts on a neuron cell membrane, dynamically regulates opening of a mechanically sensitive ion channel on the surface of the neuron cell membrane, realizes internal flow of calcium ions and the like, and thus completes precise activation and function regulation of the neuron at a subcellular precision.The nerve regulation mode of the application has the characteristics of non-contact and flexible precision, is expected to provide a non-genetic optical method for high-precision nerve regulation, and provides technical support for understanding neuron interaction and pathogenesis and treatment of neurodegenerative diseases from a subcellular level.

This invention provides grapes CAMTA The application of genes in improving plant cold resistance belongs to the field of plant genetic engineering technology. This invention uses the Eurasian grape variety 'Elegance' as research material and clones grape varieties... CAMTA The gene, whose nucleotide sequence is shown in SEQ ID NO.1, was constructed into an expression vector. Overexpression of this gene was obtained by transforming grapes, Arabidopsis thaliana, and tomato using Agrobacterium GV3101. CAMTA Transgenic plants of the gene. The results of cold resistance verification showed that under low-temperature stress, the survival rate of transgenic grape, Arabidopsis, and tomato lines was significantly higher than that of wild types. Cell membrane stability was enhanced, reactive oxygen species accumulation was reduced, and cold resistance was significantly improved, indicating that overexpression of the gene... CAMTA Genes can significantly enhance plant cold resistance. This invention provides key functional genes and technical support for cold-resistant molecular breeding of grapes and other plants, and has important application value in the selection of cold-resistant varieties.

The technical idea of the present invention provides a genetic material detection apparatus comprising: a substrate for accommodating, on a main surface thereof, a sample in which cells are suspended; a first electrode disposed on the main surface of the substrate; a second electrode disposed to face the first electrode; a first insulating film disposed on an upper surface of the first electrode and having a first window exposing a portion of the upper surface of the first electrode; a second insulating film disposed on an upper surface of the second electrode and having a second window exposing a portion of the upper surface of the second electrode; a power source configured to apply an alternating current voltage to the first electrode and the second electrode to generate an electric field for trapping the cells within the first window or the second window, and then to increase a peak voltage of the alternating current voltage to rupture membranes of the cells so as to extract genetic material; and a biosensor including a probe capable of binding to the genetic material.

The present invention relates to a composition for inducing cell membrane expression of TMC1 and a method for inducing cell membrane expression of TMC1 using same. By fusing a fragment of the GAP-43 protein to TMC1 to induce artificial palmitoylation, TMC1 can be stably expressed on the cell surface. Accordingly, the present invention is expected to be advantageously used in the development of new drugs for various diseases related to the activity, function, and expression abnormality of TMC1 by efficiently simulating the in vivo expression pattern of TMC1 to enable functional experiments thereof at the cellular level.

A microfluidic chip for delivering a payload to cells includes a plurality of constrictions configured to allow a cell suspension to flow from a first fluid flow region to a second fluid flow region within the microfluidic chip through one or more constrictions, wherein a cross-sectional width of each of the plurality of constrictions is smaller than a diameter of a cell in the cell suspension, whereby the membrane of the cell is perturbed as it passes through the constriction such that the payload can pass through the perturbed cell membrane, and wherein a quotient of the cross-sectional area to the cross-sectional perimeter of each of the plurality of constrictions is greater than or equal to 0.5 μm.

This invention discloses a method for obtaining ganglion cells through induction and enrichment of human embryonic stem cells. The method includes the following steps: knocking in genes encoding cell membrane markers and fluorescent proteins into the genome of human embryonic stem cells to obtain a reporter cell line; subjecting the reporter cell line to three-dimensional induction to obtain human retinal organoids; and sorting ganglion cells endogenously expressing cell membrane markers and fluorescent proteins from the human retinal organoids. This application provides an efficient method for obtaining ganglion cells and has significant application value.