352 results about "Cellular membrane" patented technology

The invention discloses a label-free nano plastic and single cell interaction monitoring imaging method which comprises the following steps: placing a sensing chip attached to cells on an SPRM, and recording a cell SPR image in the interaction process of the cells on the sensing chip and nano plastic; after the cell SPR image and the cell bright field image are compared to determine the corresponding area of the single cell in the SPR image, the deformation data of the cell membrane in the interaction process of the single cell and the nano plastic are obtained according to the SPR signal intensity of the area of the single cell, and the monitoring of the interaction of the nano plastic and the single cell under the resolution of the single cell is realized. The technical support is provided for researching the binding kinetics and cytotoxicity of the nano plastic.

The invention relates to dithiolane modified beta-cyclodextrin, a preparation method thereof and application of the dithiolane modified beta-cyclodextrin as a drug carrier. The method comprises the following steps: carrying out acylation reaction on beta-cyclodextrin and lipoic acid, and modifying different numbers of dithiolane on the beta-cyclodextrin by changing a feeding ratio; the beta-cyclodextrin has a plurality of hydroxyl groups, and the activity of the hydroxyl groups at the C6 position is relatively high. The hydrophobic cavity of the dithiolane-modified beta-cyclodextrin can be loaded with a hydrophobic small-molecule drug, and the hydrophobic small-molecule drug can be loaded into the dithiolane-modified beta-cyclodextrin under the condition of heating reflux to form a nano-drug. The disulfide bond on the surface of the nano-drug and sulfydryl on protein or lipid on the outer side of a cell membrane are subjected to dynamic covalent thiol exchange, so that the nano-drug enters cells through a thiol-mediated cellular uptake way, endosomes and lysosomes can be avoided, the drug is quickly and directly delivered to the cytoplasm, degradation of the drug in the lysosomes is reduced, and the drug delivery efficiency is improved. The utilization rate of small-molecule drugs is improved, side effects are reduced, and better curative effects are achieved.

The invention discloses a specific cell targeted hybrid nano-delivery carrier and a preparation method and application thereof, and aims to solve the problems of insufficient specificity, off-target risk, low delivery efficiency and the like of an existing drug delivery system. And a bionic hybrid nano delivery platform with a natural targeting function and high drug loading capacity is constructed. The vector retains key receptor molecules and signal markers on a source cell membrane, and can actively identify and target corresponding cells; meanwhile, due to the introduction of the lipidosome, the yield and stability of the material are remarkably improved, and the immune clearance rate is reduced. Experimental results show that the nano-carrier shows good targeting and biocompatibility in various disease models, and has wide clinical application prospects in the aspects of tumor treatment, tissue repair, inflammation control, targeted imaging and the like.

The invention relates to the technical field of nano-liposomes, and discloses cardamonin nano-liposomes for promoting transdermal absorption and a preparation method and application thereof, the cardamonin nano-liposomes comprise an inner phase and an outer phase, the inner phase comprises the following raw materials in parts by weight: 5-10 parts of cardamonin, 2-10 parts of lecithin and 5-20 parts of grease, and the outer phase comprises the following raw materials in parts by weight: 5-10 parts of cardamonin, 2-10 parts of lecithin and 5-20 parts of grease; the stabilizer is carboxymethyl chitosan; an external phase: 0.1-5 parts of a surfactant, 0.1-3 parts of a stabilizer, 10-20 parts of short-chain alcohol and 44.9-80 parts of water; the mixture of the external phase and the internal phase is sequentially subjected to homogenization treatment and microjet homogenization treatment, and the cardamonin nano-liposome for promoting transdermal absorption is prepared. The cardamonin nano-liposome for promoting transdermal absorption provided by the invention is a flexible liposome, and can moisten skin and reduce moisture loss on the skin surface; the transdermal amount and retention amount of the carried components are obviously improved; the permeability of the cardamonin to the skin cell membrane is enhanced.

The present application discloses a method for injecting exogenous substances into cells based on a single-cell operation device. The single-cell operation device includes a stage carrying a sample, a first probe, a first electrode arranged on the first probe, and a second electrode electrically connected to the sample. The first probe is an elongated hollow structure and includes a front end and a rear end. The front end of the first probe acts on the cell membrane of the target cell in the sample. The method for injecting exogenous substances into cells includes: step S100, establishing a first electric field between the first electrode and the second electrode, and the first probe detects the change of the first electric field signal during the movement to determine whether the front end of the first probe is in contact with the target cell; step S200, the front end of the first probe moves relative to the sample to penetrate the cell membrane of the target cell; step S300, establishing a second electric field between the first electrode and the second electrode, and the exogenous substance is injected into the target cell under the action of the second electric field. The technical solution in the present application has the advantages of high precision, low damage, high throughput, and multifunctionality.

The invention relates to a preparation method of core-shell nanoparticles with photo-thermal-phase change dual functions and intracellular delivery application of the core-shell nanoparticles, and belongs to the field of nano materials and cell delivery. The invention provides a photothermal-phase change bifunctional core-shell nanoparticle applied to intracellular delivery of biomacromolecules, the nanoparticle is of a core-shell structure, a high-molecular polymer is taken as a shell, liquid fluorocarbon and a photothermal material are taken as a core, the nanoparticle is prepared by an ultrasonic emulsification method, and the preparation method is simple. The boiling point of the liquid fluorocarbon is low, and the photo-thermal material has good photo-thermal conversion performance, so that the core-shell nanoparticles can quickly generate photo-thermal steam microbubbles under laser induction. Under the synergistic effect of laser and core-shell nanoparticles, generated steam microbubbles are induced to generate micropores in plant cell walls and cell membranes or animal cell membranes, so that the physical barrier of the cell walls and the cell membranes is overcome, and the efficiency of delivering exogenous functional biomacromolecules into cells is remarkably improved.

A genome-editing complex for modifying a target polynucleotide comprising a recombinant β helical protein linked to either one or more molecules of a genome-editing system or a plasmid encoding for one or more molecules of a genome-editing system, wherein the β helical protein length is in the range of from 5 nm to 25 nm, and width is in the range of from 1 nm to 5 nm.

The invention relates to a striker type micro-nano robot for cell membrane breakthrough and a preparation process of the striker type micro-nano robot, in particular to a striker type micro-nano robot driving control method based on a photomagnetic coupling field and a rotary blasting type cell membrane breakthrough method. The invention discloses a striker type micro-nano robot for cell membrane breakthrough. The striker type micro-nano robot consists of a magnetic iron oxide core and a silicon dioxide needle handle, wherein the surface of one side of the magnetic iron oxide core is coated with a gold layer. The invention discloses a preparation process of a firing pin type micro-nano robot. The preparation process of the firing pin type micro-nano robot comprises the steps of sol-gel core preparation, magnetron sputtering and chemical precipitation needle handle preparation. Under the cooperative driving of an external magnetic field and a near-infrared light field, rotary blasting type breakthrough can be performed on a mononuclear membrane through the tip end of the needle handle.

The invention discloses a cell fate distinguishing and differentiation prediction method based on a three-dimensional form, and belongs to the technical field of cell fate distinguishing and differentiation prediction. In order to solve the problem that cell fate differentiation is difficult to distinguish and predict under a small sample size, the method comprises the following steps: acquiring a living biological sample, and imaging by using a three-dimensional delay microscope to obtain cell three-dimensional image data; segmenting the cell membrane boundary and extracting geometric and high-order three-dimensional morphological characteristic parameters; labeling cell fate and differentiation states based on means such as cell lineage tracking or gene fluorescence labeling; and finally, determining an optimal morphological index and a minimum sample size through single-side double-sample t test, and applying the optimal morphological index and the minimum sample size to two new cell populations and outputting a cell fate distinguishing and differentiation prediction result. The method can realize cross-species, low-loss, low-cost and high-precision cell fate differentiation and differentiation prediction, and is suitable for development research, stem cell culture and disease diagnosis.

The invention provides a hybrid bionic nano delivery system and a preparation method thereof. The hybrid bionic nano delivery system is composed of a carbon dot doped nitrogen carbide-based nanoparticle core and a cell membrane group wrapping the nitrogen carbide-based nanoparticle core. The invention constructs a hybrid bionic nano delivery system, which has good biocompatibility and biological metabolism, is beneficial to application in the field of biomedicine, and can realize low-temperature photothermal therapy of hepatic fibrosis. The carbon nitride-based nano bionic system constructed by the invention is mainly synthesized by high-temperature calcination, ultrasonic treatment and hybrid cell membrane wrapping. The preparation method disclosed by the invention is simple, has good repeatability and has a relatively good industrial production prospect.

The invention discloses a polypeptide nanoparticle composition, which relates to the gene delivery technology field in the field of gene therapy. The peptide for delivering nucleic acid has the following general structure: (Arg)n-Xa-Yb-Xc-Cys (general formula I), wherein: n+a+b+c+1=6-40, n, a, b or c are independent of each other, and n≥1, b≥1, a, c are any of 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26. The invention provides a novel non-natural polypeptide having the function of encapsulating nucleic acid and selectively promoting nucleic acid to pass through cell membrane, as well as a macromolecular lipid polypeptide nanoparticle containing the polypeptide, a method for using the same for in vivo and in vitro cell gene transfection, and a method for applying the same to vaccine preparations.

The invention belongs to the technical field of cell biology, and particularly relates to a preparation method of tumor enhanced targeting and therapeutic function extracellular vesicles and a chip device. The invention firstly provides an integrated chip device which is provided with a cell screening module and a functional preparation module and comprises a plurality of functional layers, wherein one functional layer is provided with a cell evolution screening area with gradient for cell screening, and the other functional layer is provided with a cell electric remodeling area for cell functionalization transformation. The invention innovatively proposes that tumor cells with strong metastasis ability are screened based on culture gradient, the tumor cells with stronger invasion ability are distinguished by utilizing spatial segmentation, then the screened cell subpopulation is transferred to a cell electric remodeling area in combination with overturning operation of the chip, and the cell subpopulation is transferred to the cell electric remodeling area in a manner of focusing an electric field through nanopores. And meanwhile, therapeutic molecules are efficiently delivered to realize cell transformation, so that the transformed cells can enhance the output of extracellular vesicles with a therapeutic function.

The invention relates to a plasma active group and microbial cell membrane action analysis system, which comprises a plasma active group diagnosis module used for acquiring plasma active group data under different conditions, and the group data comprises the type, concentration and spatial distribution of active groups; the microbial characterization analysis module is used for acquiring cell membrane data before and after plasma treatment, and the cell membrane data comprises morphological structure, component and functional change of cell membranes; and the machine learning data analysis module is used for carrying out data preprocessing, feature extraction and importance sorting on the acquired plasma active group data and cell membrane data so as to select important features to carry out regression analysis and obtain a nonlinear correlation model of the action of the plasma active group and the cell membrane. Compared with the prior art, the method has the advantages that the interaction effect and mechanism of the plasma active group and the microbial cell membrane can be quickly and accurately analyzed and predicted, and the research efficiency and precision are remarkably improved.

The invention belongs to the technical field of biological medicine, and particularly relates to a novel recombinant protein TAT-FLG2 with cell membrane penetrating capacity and application. The novel recombinant protein TAT-FLG2 provided by the invention comprises a part of peptide fragment of silk protein 2 and a section of cell-penetrating peptide which are connected through a section of flexible connexon. The three-dimensional structure of the novel recombinant protein TAT-FLG2 has a shape similar to a V shape. The novel recombinant protein TAT-FLG2 provided by the invention has the capability of efficiently penetrating cell membranes and the capability of promoting the migration of fibroblasts.

The invention relates to the technical field of medical biology, and discloses a composition for promoting interaction of collagen and hyaluronic acid, which comprises collagen, hyaluronic acid and a chemical cross-linking agent. According to the preparation method of the composition for promoting interaction of the collagen and the hyaluronic acid and the application of the chemical cross-linking agent N (beta maleimido) propionate, a stable chemical bond is formed between the collagen and the hyaluronic acid, and the molecular structure is optimized, so that the composition is easier to penetrate through the cuticle of the skin, and the interaction of the collagen and the hyaluronic acid is promoted. Physical treatment of ultrasonic waves and a high-voltage pulse electric field accelerates formation of a compound, the skin barrier is reduced through mechanical and electroporation effects, conditions are provided for deep penetration of the compound, particles are refined through the cavitation effect of the ultrasonic waves, and uniformity and fluidity are improved; the electroporation effect of the high-voltage pulsed electric field increases the permeability of cell membranes, an additional permeation path is provided for the compound, and the comprehensive measures effectively improve the transdermal absorption efficiency and the biological activity of the compound.

A microneedle or microneedle device includes a microneedle body extending from a base to a penetrating tip formed from a silk fibroin based material, which is easy to fabricate and highly biocompatible. The microneedle device can include one or more microneedles mounted to a substrate. The silk fibroin can include active agents to be transported into or across biological barriers such as skin, tissue and cell membranes. The silk fibroin microneedles can be fully or partially biodegradable and / or bioerodible. The silk fibroin is highly stable, affords room temperature storage and is implantable. The silk fibroin structure can be modulated to control the rate of active agent delivery.

The present disclosure, in some aspects, is directed to high-throughput systems, and methods of use thereof, for intracellular delivery of one or more payloads to separate cell samples. For example, in certain aspects, provided is a high-throughput liquid handler configured for distribution of mechanically perturbed cells in formats suitable for high-throughput cellular assays. In certain other aspects, the present disclosure is directed to components of said high-throughput systems, e.g., liquid handlers and cartridges comprising a filter comprising a plurality of pores therethrough for perturbing cell membranes, and uses of said high-throughput systems.

The invention provides a yellow virus replicon (sef-replicating mRNA (messenger ribonucleic acid), srRNA (srRNA)) vector with a plurality of structures aiming at functional localization of different therapeutic proteins. Recombinant sites and structural characteristics of the srRNAs and carrying target genes are explained in detail, therapeutic proteins are expressed according to functions of the therapeutic proteins and are correctly distributed inside and outside cells or embedded into cell membranes, the half-life period and stability of srRNA expression are improved, and the srRNAs are effectively applied to gene therapy of diseases.

The present disclosure pertains to a circulating tumor cell (CTC) microfluidic platform that is used for the detection of CTCs, enumeration of CTCs in a sample, characterization of biophysical properties, CTC cell size, CTC cell membrane deformability, stresses on CTC cell membranes, adhesion stress on CTC cells, normal stress of CTC cells, or combinations thereof.

Described herein are localization polypeptides, RNA carrier polypeptides, polynucleotides, dimerization handles, cells, compositions, methods, and kits relating to the spatial localization of RNA in relation to a cell. In certain aspects, localization polypeptides and RNA carrier polypeptides may interact to carry an RNA of interest to a subcellular location or other location associated with a cellular membrane. In embodiments, such interaction may be covalent, for example, by the way of translational fusion, or may be inducible through a dimerization handle as described herein. In embodiments, RNA localization can be carried out on (and cells of the present disclosure can be) asymmetric cells, for example, neurons or neural progenitors. In embodiments, the ABI / PYL system can be utilized in conjunction with a catalytically-dead (i.e., nuclease-inactivated) RNA-guided CAS enzyme to precisely spatially localize an RNA of interest to a subcellular location of interest.

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.

The present application belongs to the technical field of daily chemical preparation, and particularly relates to a gynecological gel for inhibiting bacteria, regulating microecological balance and tightening vagina and a preparation method thereof. The gynecological gel comprises the following raw materials in mass fractions: 0.5-1 parts of carbomer, 0.04-0.09 parts of sodium hydroxide, 1-3 parts of collagen, 0.01-0.06 parts of sodium hyaluronate, 0.5-1.5 parts of isomaltooligosaccharide, 2-5 parts of propylene glycol, 0.05-0.3 parts of hydroxybenzoic acid methyl ester, 0.02-0.15 parts of hydroxyphenyl ethyl ester, 0.1-1.5 parts of hydrogenated lecithin, 1-6 parts of 1,3-butanediol, 3-5 parts of glycerol, 0.5-1 part of xanthan gum and 70-90 parts of water. The present application uses buckwheat filtrate to prepare collagen, and the active ingredients in the buckwheat filtrate can penetrate the cell membrane of pathogenic bacteria to play an antibacterial role; the collagen can repair the vaginal mucosa barrier and reduce the adhesion and invasion of pathogenic bacteria; therefore, after being compounded with the raw materials such as carbomer, sodium hydroxide, sodium hyaluronate, isomaltooligosaccharide and propylene glycol, the multiple components can synergistically promote the synthesis and repair of collagen fibers and improve the elasticity of tissues.

This invention provides a multifunctional nanodelivery system integrating gene delivery and CT tracking. The multifunctional nanodelivery system includes a gene delivery vector and an HGF plasmid gene loaded on the gene delivery vector; the gene delivery vector comprises gold nanoparticles coated with protamine sulfate. The gene delivery vector AuPS provided by this invention has excellent cell membrane transport and nuclear localization capabilities, enabling the HGF plasmid gene to efficiently enter cells and be expressed in the nucleus. The multifunctional nanodelivery system provided by this invention can be used for cell function regulation and tracking of therapeutic cells such as stem cells.

A microfluidic chip for causing the delivery of a payload to a cell comprises a plurality of constrictions configured to allow a cell suspension to flow through one or more of the plurality of constrictions from a first fluid flow region to a second fluid flow region within the microfluidic chip, wherein a cross-sectional width of each of the plurality of constrictions is less than a diameter of cells in the cell suspension, such that membranes of the cells are perturbed when passing through the constrictions such that a payload is able to pass through the perturbed cell membranes, and wherein a quotient of a cross-sectional area over a cross-sectional perimeter of each of the plurality of constrictions is greater than greater than or equal to 0.5 μm.

The present disclosure relates to devices, e.g, extracorporeal blood purification (EBP) devices, which comprise a cartridge or sorbent comprising a cellular membrane derived from a cell. The present disclosure also relates methods for removing or reducing a substance, e.g, an undesirable substance, from a fluid, e.g, a subject's blood, using the devices or EBP devices.

The invention discloses a PavSWEET9b gene for regulating and controlling the sugar content of sweet cherry fruits and application of the PavSWEET9b gene, and belongs to the technical field of gene engineering. The nucleotide sequence of the PavSWEET9b gene for regulating and controlling the sugar content of the sweet cherry fruits is shown as SEQ ID NO.1. The research finds that the PavSWEET9b protein is positioned on a cell membrane, and the soluble sugar content of the sweet cherry fruits is correspondingly reduced or increased after the gene is silenced and overexpressed, so that the PavSWEET9b gene can regulate and control the soluble sugar accumulation of the sweet cherry fruits; according to the method, the content of sugar in the sweet cherry fruits can be increased, so that the fruit quality is improved, the taste of the sweet cherry fruits is improved, the requirements of consumers are better met, and a new idea is provided for development of new sweet cherry varieties.

An antibacterial artificial nano-cell and a preparation method therefor. The antibacterial artificial nano-cell is prepared by the following steps: mixing polyvinylpyrrolidone or an aqueous solution thereof, CuCl2·2H2O or an aqueous solution thereof, FeCl2·4H2O or an aqueous solution thereof, Elesclomol or a DMSO solution thereof, NaOH or an aqueous solution thereof, and H2O2 or an aqueous solution thereof, uniformly stirring the mixture, followed by washing, ultrafiltration and centrifugation to obtain nanoparticles, uniformly mixing the nanoparticles with cell membranes prepared from bone marrow stromal cells, and carrying out ultrasonic incubation and extrusion to obtain the antibacterial artificial nano-cells.

To introduce material to cells, contemporary medicinal constructs rely on the uptake mechanisms of the cell membrane. This puts major restrictions on the types of utilizable materials (e.g., charge compatible), specifications (e.g., 100 nanometer scale or less) and organizations (mostly simplistic spheroids); this is the regime of nanoparticles, protein / peptide conjugates etc. However, the focus and novelty of the innovation presented are constructs which can still achieve this membrane interaction to connect to cells yet the constructs themselves remain outside of the cell, thus establishing a network by which to transfer materials. These can surpass the aforementioned limitations as well as create entirely new application spaces as these new constructs enable different desired distribution patterns and exchanged material.

1. Name of the product in this design: Cell membrane potential modulation machine. 2. Purpose of this design: To apply the principle of biomimetic pulsed magnetic resonance to regulate cell membrane potential, thereby improving sleep and regulating sub-health conditions. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The application belongs to the technical field of biological medicine, and particularly relates to a kind of composite nanoparticles and its preparation method and application.The composite nanoparticles provided by the application include nano calcium peroxide, hyaluronic acid coated on the surface of the nano calcium peroxide and urease coated on the surface of the hyaluronic acid.In the application, the hyaluronic acid has tumor cell targeting property, which is conducive to accurately delivering the composite nanoparticles to tumor cells;at the same time, the hyaluronic acid can fix the urease on the surface of the nano calcium peroxide, which is conducive to improving the biological mineralization rate.In the application, when the composite nanoparticles enter the tumor microenvironment under the condition of providing urea outside, they are affected by the acidic microenvironment, the nano calcium peroxide decomposes to release calcium ions, and at the same time, the urease specifically decomposes urea in the microenvironment to release carbonate ions, the calcium ions and carbonate ions produce a mineral layer on the surface of the cell membrane through synergistic effect, thereby blocking the channel of cell material exchange and inducing cell apoptosis.