194 results about "Cellular membrane" patented technology

The invention discloses a specific cell targeted hybrid nano-delivery carrier and a preparation method and application thereof, and aims to solve the problems of insufficient specificity, off-target risk, low delivery efficiency and the like of an existing drug delivery system. And a bionic hybrid nano delivery platform with a natural targeting function and high drug loading capacity is constructed. The vector retains key receptor molecules and signal markers on a source cell membrane, and can actively identify and target corresponding cells; meanwhile, due to the introduction of the lipidosome, the yield and stability of the material are remarkably improved, and the immune clearance rate is reduced. Experimental results show that the nano-carrier shows good targeting and biocompatibility in various disease models, and has wide clinical application prospects in the aspects of tumor treatment, tissue repair, inflammation control, targeted imaging and the like.

The invention relates to a striker type micro-nano robot for cell membrane breakthrough and a preparation process of the striker type micro-nano robot, in particular to a striker type micro-nano robot driving control method based on a photomagnetic coupling field and a rotary blasting type cell membrane breakthrough method. The invention discloses a striker type micro-nano robot for cell membrane breakthrough. The striker type micro-nano robot consists of a magnetic iron oxide core and a silicon dioxide needle handle, wherein the surface of one side of the magnetic iron oxide core is coated with a gold layer. The invention discloses a preparation process of a firing pin type micro-nano robot. The preparation process of the firing pin type micro-nano robot comprises the steps of sol-gel core preparation, magnetron sputtering and chemical precipitation needle handle preparation. Under the cooperative driving of an external magnetic field and a near-infrared light field, rotary blasting type breakthrough can be performed on a mononuclear membrane through the tip end of the needle handle.

The invention discloses a cell fate distinguishing and differentiation prediction method based on a three-dimensional form, and belongs to the technical field of cell fate distinguishing and differentiation prediction. In order to solve the problem that cell fate differentiation is difficult to distinguish and predict under a small sample size, the method comprises the following steps: acquiring a living biological sample, and imaging by using a three-dimensional delay microscope to obtain cell three-dimensional image data; segmenting the cell membrane boundary and extracting geometric and high-order three-dimensional morphological characteristic parameters; labeling cell fate and differentiation states based on means such as cell lineage tracking or gene fluorescence labeling; and finally, determining an optimal morphological index and a minimum sample size through single-side double-sample t test, and applying the optimal morphological index and the minimum sample size to two new cell populations and outputting a cell fate distinguishing and differentiation prediction result. The method can realize cross-species, low-loss, low-cost and high-precision cell fate differentiation and differentiation prediction, and is suitable for development research, stem cell culture and disease diagnosis.

The invention provides a hybrid bionic nano delivery system and a preparation method thereof. The hybrid bionic nano delivery system is composed of a carbon dot doped nitrogen carbide-based nanoparticle core and a cell membrane group wrapping the nitrogen carbide-based nanoparticle core. The invention constructs a hybrid bionic nano delivery system, which has good biocompatibility and biological metabolism, is beneficial to application in the field of biomedicine, and can realize low-temperature photothermal therapy of hepatic fibrosis. The carbon nitride-based nano bionic system constructed by the invention is mainly synthesized by high-temperature calcination, ultrasonic treatment and hybrid cell membrane wrapping. The preparation method disclosed by the invention is simple, has good repeatability and has a relatively good industrial production prospect.

The invention relates to a plasma active group and microbial cell membrane action analysis system, which comprises a plasma active group diagnosis module used for acquiring plasma active group data under different conditions, and the group data comprises the type, concentration and spatial distribution of active groups; the microbial characterization analysis module is used for acquiring cell membrane data before and after plasma treatment, and the cell membrane data comprises morphological structure, component and functional change of cell membranes; and the machine learning data analysis module is used for carrying out data preprocessing, feature extraction and importance sorting on the acquired plasma active group data and cell membrane data so as to select important features to carry out regression analysis and obtain a nonlinear correlation model of the action of the plasma active group and the cell membrane. Compared with the prior art, the method has the advantages that the interaction effect and mechanism of the plasma active group and the microbial cell membrane can be quickly and accurately analyzed and predicted, and the research efficiency and precision are remarkably improved.

The invention provides a streptomyces flavogriseus C-21 strain, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the registration number of the streptomyces flavogriseus C-21 strain is CGMCC No. 36050. The preparation method of the sterile fermentation filtrate of the streptomyces fulvus C-21 comprises the following steps: inoculating a strain C-21 to an oat culture medium plate in a streak manner, culturing for 7 days at 28 DEG C, scraping spores on the surface of the culture medium, adding sterile water to prepare a spore suspension with the concentration of 108 / mL, inoculating the spore suspension into a millet immersion culture solution according to the inoculum size of 2%, and culturing for 7 days at the temperature of 28 DEG C to obtain the sterile fermentation filtrate of the streptomyces fulvus C-21. Performing shaking culture for 7 days in a constant-temperature shaking table at the temperature of 28 DEG C and the speed of 180 r / min, centrifuging for 30 minutes at the speed of 12000 r / min, and filtering supernate by using a 0.22 mu m microporous filter membrane to obtain sterile fermentation filtrate. The streptomyces fulvus C-21 can generate extracellular enzymes such as cellulase, protease and amylase, sterile fermentation filtrate of the streptomyces fulvus C-21 can destroy mycelial morphology and cell membrane integrity of the tomato late blight bacteria, mycelial growth, sporangium germination and zoospore release of the tomato late blight bacteria are remarkably inhibited, and the streptomyces fulvus C-21 has a good control effect on the tomato late blight.

The present application belongs to the technical field of daily chemical preparation, and particularly relates to a gynecological gel for inhibiting bacteria, regulating microecological balance and tightening vagina and a preparation method thereof. The gynecological gel comprises the following raw materials in mass fractions: 0.5-1 parts of carbomer, 0.04-0.09 parts of sodium hydroxide, 1-3 parts of collagen, 0.01-0.06 parts of sodium hyaluronate, 0.5-1.5 parts of isomaltooligosaccharide, 2-5 parts of propylene glycol, 0.05-0.3 parts of hydroxybenzoic acid methyl ester, 0.02-0.15 parts of hydroxyphenyl ethyl ester, 0.1-1.5 parts of hydrogenated lecithin, 1-6 parts of 1,3-butanediol, 3-5 parts of glycerol, 0.5-1 part of xanthan gum and 70-90 parts of water. The present application uses buckwheat filtrate to prepare collagen, and the active ingredients in the buckwheat filtrate can penetrate the cell membrane of pathogenic bacteria to play an antibacterial role; the collagen can repair the vaginal mucosa barrier and reduce the adhesion and invasion of pathogenic bacteria; therefore, after being compounded with the raw materials such as carbomer, sodium hydroxide, sodium hyaluronate, isomaltooligosaccharide and propylene glycol, the multiple components can synergistically promote the synthesis and repair of collagen fibers and improve the elasticity of tissues.

This invention provides a multifunctional nanodelivery system integrating gene delivery and CT tracking. The multifunctional nanodelivery system includes a gene delivery vector and an HGF plasmid gene loaded on the gene delivery vector; the gene delivery vector comprises gold nanoparticles coated with protamine sulfate. The gene delivery vector AuPS provided by this invention has excellent cell membrane transport and nuclear localization capabilities, enabling the HGF plasmid gene to efficiently enter cells and be expressed in the nucleus. The multifunctional nanodelivery system provided by this invention can be used for cell function regulation and tracking of therapeutic cells such as stem cells.

A microfluidic chip for causing the delivery of a payload to a cell comprises a plurality of constrictions configured to allow a cell suspension to flow through one or more of the plurality of constrictions from a first fluid flow region to a second fluid flow region within the microfluidic chip, wherein a cross-sectional width of each of the plurality of constrictions is less than a diameter of cells in the cell suspension, such that membranes of the cells are perturbed when passing through the constrictions such that a payload is able to pass through the perturbed cell membranes, and wherein a quotient of a cross-sectional area over a cross-sectional perimeter of each of the plurality of constrictions is greater than greater than or equal to 0.5 μm.

The present disclosure relates to devices, e.g, extracorporeal blood purification (EBP) devices, which comprise a cartridge or sorbent comprising a cellular membrane derived from a cell. The present disclosure also relates methods for removing or reducing a substance, e.g, an undesirable substance, from a fluid, e.g, a subject's blood, using the devices or EBP devices.

The invention discloses a PavSWEET9b gene for regulating and controlling the sugar content of sweet cherry fruits and application of the PavSWEET9b gene, and belongs to the technical field of gene engineering. The nucleotide sequence of the PavSWEET9b gene for regulating and controlling the sugar content of the sweet cherry fruits is shown as SEQ ID NO.1. The research finds that the PavSWEET9b protein is positioned on a cell membrane, and the soluble sugar content of the sweet cherry fruits is correspondingly reduced or increased after the gene is silenced and overexpressed, so that the PavSWEET9b gene can regulate and control the soluble sugar accumulation of the sweet cherry fruits; according to the method, the content of sugar in the sweet cherry fruits can be increased, so that the fruit quality is improved, the taste of the sweet cherry fruits is improved, the requirements of consumers are better met, and a new idea is provided for development of new sweet cherry varieties.

An antibacterial artificial nano-cell and a preparation method therefor. The antibacterial artificial nano-cell is prepared by the following steps: mixing polyvinylpyrrolidone or an aqueous solution thereof, CuCl2·2H2O or an aqueous solution thereof, FeCl2·4H2O or an aqueous solution thereof, Elesclomol or a DMSO solution thereof, NaOH or an aqueous solution thereof, and H2O2 or an aqueous solution thereof, uniformly stirring the mixture, followed by washing, ultrafiltration and centrifugation to obtain nanoparticles, uniformly mixing the nanoparticles with cell membranes prepared from bone marrow stromal cells, and carrying out ultrasonic incubation and extrusion to obtain the antibacterial artificial nano-cells.

1. Name of the product in this design: Cell membrane potential modulation machine. 2. Purpose of this design: To apply the principle of biomimetic pulsed magnetic resonance to regulate cell membrane potential, thereby improving sleep and regulating sub-health conditions. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The application belongs to the technical field of biological medicine, and particularly relates to a kind of composite nanoparticles and its preparation method and application.The composite nanoparticles provided by the application include nano calcium peroxide, hyaluronic acid coated on the surface of the nano calcium peroxide and urease coated on the surface of the hyaluronic acid.In the application, the hyaluronic acid has tumor cell targeting property, which is conducive to accurately delivering the composite nanoparticles to tumor cells;at the same time, the hyaluronic acid can fix the urease on the surface of the nano calcium peroxide, which is conducive to improving the biological mineralization rate.In the application, when the composite nanoparticles enter the tumor microenvironment under the condition of providing urea outside, they are affected by the acidic microenvironment, the nano calcium peroxide decomposes to release calcium ions, and at the same time, the urease specifically decomposes urea in the microenvironment to release carbonate ions, the calcium ions and carbonate ions produce a mineral layer on the surface of the cell membrane through synergistic effect, thereby blocking the channel of cell material exchange and inducing cell apoptosis.

This invention relates to the field of embryo resuscitation technology and discloses an auxiliary warming device for frozen embryo resuscitation, including a collection plate. A fixing ring is fixedly connected to the side wall of the collection plate, and a forward threaded groove is formed on the side wall of the fixing ring. The cover plate drives the separator ring to rotate clockwise through the contact rod and the fixing plate. Since the reverse threaded groove rotates in the opposite direction to the forward threaded groove, the rotating ring will move upward at this time. The upward rotating ring will drive the separator ring to move upward synchronously, so that the through hole gap coincides with the flow hole. At this time, the new solution on the outer wall of the separator ring flows downward through the flow hole and the through hole gap. Finally, the new solution mixes with the solution inside the collection plate. At this time, the pressure of the dripping will be absorbed by the top of the fixing ring and will not directly contact the solution inside the collection plate. When the operator twists the cover plate to close it, the flow hole and the through hole gap will coincide, and the new solution will mix with the solution inside the collection plate, effectively reducing the damage to the outer wall of the embryo cell membrane when injecting the new solution.

This invention relates to the field of biomedical technology, specifically to a microfluidic channel, a microfluidic chip, and their applications for squeezing cells. The microfluidic channel is composed of several continuously arranged cell squeezing units. Each cell squeezing unit consists of a main channel and a squeezing region. The main channel is composed of connecting sections and curved sections. The squeezing region includes a first squeezing section, a squeezing slit, and a second squeezing section. The width of the first squeezing section gradually decreases from the main channel, reaching its minimum at the squeezing slit, and then gradually increases towards the second squeezing section until it matches the width of the main channel. The squeezing slit is rectangular, with a width of 4-10 μm and a length of 20-60 μm. The microfluidic channel provided by this invention applies relatively uniform and dispersed mechanical stress to the cell membrane, reducing cell breakage and mortality rates, and allowing cells to restore their integrity through the cell membrane's self-repair mechanism after squeezing.

This invention relates to the fields of brachytherapy and radiosensitization, specifically providing a... 125 I-nano implantable particles, their preparation methods, and applications. 125 The 1-nanometer implanted particles have a core-shell structure, consisting of a particle core and particles adsorbed on the outer surface of the particle core. 125 I and the particles encapsulated in the nucleus and 125 The outer shell of the I-type photosensitizer is a covalent organic framework. This invention... 125 The core of the I-nanometer implanted particle is used to enhance the energy deposition of gamma rays and X-rays, generating more hydroxyl radicals; the shell containing a type I photosensitizer is used to absorb the energy of Auger electrons and internal conversion electrons and excite the photosensitizer to generate singlet oxygen, effectively improving the response to... 125 The efficiency of I decay energy utilization. 125 I-nano implanted particles, combined with internal radiation therapy and radiodynamic therapy, destroy tumor cell DNA and cell membranes, enhance tumor killing and induce immunogenic cell death, thereby activating anti-tumor immunity and inducing remote effects.

A computational system and method for digital modeling of cardiac cell membrane electrophysiology is disclosed. The system generates a digital model representing the cell membrane as a dielectric lipid bilayer comprising a capacitor element and a resistor element, where the resistor element is emulated by a plurality of ion channels. The system calculates cell membrane potential based on intracellular and extracellular concentrations of key ions, such as Na+, K+, Ca2+, and Cl-, using the Goldman-Hodgkin-Katz equation. Generation of cellular membrane action potentials is simulated by modeling the opening and closing of voltage-gated ion channels in response to triggering events. The model provides dynamic visualizations of the distinct phases of the action potential, including depolarization and repolarization, offering a high-fidelity tool for research and for enhancing the realism of training simulations in electrophysiology procedures.

The invention discloses a near-infrared ratio type fluorescent probe for rapidly and reversibly detecting histamine (HA), a preparation method of the fluorescent probe and application of the fluorescent probe in dynamic cell imaging, and belongs to the technical field of fluorescence imaging and biosensing. The preparation method comprises the following specific steps: synthesizing aza-BODIPY molecules (1a-1d, 2a-2d) with different halogenations, and meanwhile, connecting the aza-BODIPY molecules with hydroxyl groups at 3 and 5 sites with a hydrophilic PEG (Polyethylene Glycol) fragment and halogen to obtain an HA probe HAPCyto with good water solubility; in addition, the hydroxyl aza-BODIPY molecules are continuously subjected to substitution, halogenation and hydrolysis reaction to obtain carboxyl-containing aza-BODIPY molecules, then the aza-BODIPY molecules are subjected to amidation reaction by utilizing the site, and different organic fragments are respectively connected to obtain a cell membrane targeted HA probe HAPPM, a golgi apparatus targeted HA probe HAPGA and a HaloTag HA fluorescent probe HAPHaloTag. When HA exists in a solution, the developed probe can realize rapid reversible detection of the near-infrared ratio of histamine, also shows good reversible property in mast cells, and can realize dynamic monitoring of histamine in cells.

The invention relates to a mesenchymal stem cell-nano knapsack system, preparation thereof and application of the mesenchymal stem cell-nano knapsack system in treatment of cerebral arterial thrombosis. The invention constructs an engineered mesenchymal stem cell-thrombin responsive nano knapsack for sequential targeted delivery of rtPA, antioxidant active molecules and MSCs for ischemic stroke treatment. In a sequential delivery mode, firstly, a thrombus site is targeted, the thrombin is responded to release rtPA for thrombolysis, brain blood supply is recovered, and the rtPA thrombolysis efficiency is improved. The antioxidant active molecules are entrapped on the erythrocyte membrane bionic nanoparticles, are loaded on the surfaces of the MSCs in a knapsack form, penetrate through BBB and tend to damage ischemic penumbra and release the antioxidant active molecules by means of central transport and inflammatory homing effects of the MSCs, on one hand, oxidative stress is inhibited, a microenvironment beneficial to activity maintenance is provided for the MSCs, and on the other hand, the antioxidant active molecules are released; and meanwhile, the medicine cooperates with MSCs to play a role in protecting injured nerve and blood vessel units, so that the treatment effect on cerebral arterial thrombosis is improved.

A system and method of performing deep cell body segmentation on a biological sample is provided. The method includes receiving a first and a second stained image. The first image is processed using a trained machine learned model that outputs locations of a plurality of cell nuclei in the first stained image. Seed points are then determined based on the locations of the plurality of cell nuclei. The second image is then processed using the seed points to determine a plurality of cell membranes using a watershed segmentation. The second image is then post-processed and an output image is produced. The output image is then analyzed and gene sequencing is performed.

The present invention discloses a molecular dynamics simulation analysis method for ultrasonic perturbation of the Piezo1 protein, belonging to the field of ultrasonic perturbation. In the molecular dynamics simulation, ultrasonic perturbation is achieved by applying an ultrasonic function to the boundary, and successful ultrasonic loading is verified based on the water density on the X-axis. Furthermore, an analytical method is established to explain the effects of ultrasound on cell membranes from multiple angles, such as cell membrane thickness and lipid-protein interactions, and further study the effects of cell membranes on proteins, thereby determining the effects of ultrasound on the Piezo1 structure. This invention is the first to use ultrasonic loading coarse-grained molecular dynamics simulation to study the conformational changes of the Piezo1 mechanosensitive ion channel, providing important research ideas for future applications in ultrasound therapy, acoustic genetics, and other fields.

The invention relates to the technical field of fluorescent probes, in particular to a cell membrane targeted near-infrared AIE fluorescent probe as well as a preparation method and application of the cell membrane targeted near-infrared AIE fluorescent probe. Dithieno [2, 3-b: 2 ', 3'-d] thiophene is adopted as a bridging structure, a triphenylamine unit is connected with a pyridinium salt unit, and the compound is specially used for targeted cell membrane localization and photodynamic tumor treatment. The near-infrared AIE fluorescent probe disclosed by the invention can be combined with a cell membrane to realize an effect of accurately and rapidly positioning cells; and under light stimulation, I-type / II-type ROS can be generated, the cell membrane structure can be destroyed, the cell membrane vacuolation can be realized, the effective killing of in-vivo and in-vitro tumor cells can be realized, and the method has important significance on tumor treatment.

The application provides a nano-catalytic material system capable of targeting tumors for gene editing, which is obtained by loading a CRISPR / Cas9 plasmid of a gene editing system capable of editing a core protein gene GPX4 related to cell ferroptosis into the pores of an iron single-atom nano-catalyst, and then modifying the cell membrane of a homologous tumor. The nano-catalytic material system provided by the application has excellent Fenton catalytic performance, can accurately edit target genes, induces tumor cells to undergo ferroptosis, and has the advantages of simple synthesis process, low cost, easy batch production, high enrichment degree at a lesion site, good treatment effect, and good clinical application prospect.

Cancer treatment using TTFields (Tumor Therapy Fields) can be tailored to each individual subject. This involves obtaining cancer cells from the subject, determining their electrical properties (e.g., dielectric electrophoretic force, cell membrane capacitance, etc.), determining the frequency for using the TTField based on these properties, and treating the cancer by applying the TTField to the subject at the determined frequency. Furthermore, cancer treatment can be planned for each individual subject by obtaining cancer cells from the subject, determining their electrical properties, predicting whether the TTField will be effective in treating the cancer based on these properties, and treating the subject by applying the TTField if the prediction indicates its effectiveness.

A genome-editing complex for modifying a target polynucleotide comprising a recombinant β helical protein linked to either one or more molecules of a genome-editing system or a plasmid encoding for one or more molecules of a genome-editing system, wherein the β helical protein length is in the range of from 5 nm to 25 nm, and width is in the range of from 1 nm to 5 nm.

The invention belongs to the technical field of biological medicines, and relates to a high-throughput screening method based on cell membrane nano-vesicles and application of a screened drug afloqualone in resisting encephaledema. Through a screening model, it is surprisingly found that the drug afloqualone AFQ on the market has the remarkable effect of inhibiting water inflow and encephaledema. The glibenclamide pharmaceutical composition shows a curative effect superior to that of glibenclamide in an animal model, can effectively reduce the volume of cerebral infarction, reduce the death rate in an acute stage and improve neurological impairment, and is known to have good clinical safety and good clinical application prospects. The invention provides a brand-new treatment strategy with a great clinical application prospect for solving the problem of lack of effective medicines for post-stroke encephaledema at present.

The invention provides a viscosity response type fluorescent probe as well as a preparation method and application thereof, and belongs to the technical field of biochemical detection and analysis. The chemical structural formula of the fluorescent probe is shown in the specification, X represents halogen, and n is an integer between 0 and 10. The method can be applied to specific viscosity detection, targeted positioning of cell membranes and distinguishing of normal and tumor cells by utilizing the viscosity difference of the cell membranes. The probe synthesized by the invention can realize accurate detection of the viscosity change of the cell membrane.

The present invention provides: a method for preparing tissue-targeting nanoparticles, the method comprising a step for generating phospholipid nanoparticles, a step for generating mesenchymal stem cell-derived cell membrane nanoparticles, and a step for fusing the generated phospholipid nanoparticles and cell membrane nanoparticles to generate tissue-targeting nanoparticles; and tissue-targeting nanoparticles which are prepared by the method and comprise bisphosphonate-containing phospholipid nanoparticles and mesenchymal stem cell-derived cell membrane nanoparticles.

The invention relates to the field of micro-nano robots, in particular to a hexapod cell puncture micro-nano robot with complex environment adaptability. The structure comprises a core and an outer foot, the core is made of iron oxide, and the outer foot is made of silicon dioxide. The core material iron oxide of the micro-nano robot has paramagnetism and can respond to an external magnetic field; the six-foot structure generates multiple contact fulcrums in the movement process of the tissue surface with the complex morphology, rolling movement on the tissue surface with the complex morphology is facilitated, and the capacity of the micro-nano robot for adapting to the complex surface morphology of the tissue in a living body can be improved. When the micro-nano robot rolls over the cancer cell membrane along the cancer cell membrane, the magnetic field generated by the coil is adjusted to be a plane rotating magnetic field, and the micro-nano robot rotates at high frequency in the axial direction perpendicular to the horizontal plane; the gradient magnetic field device at the bottom is started, the micro-nano robot rotates and linearly moves under the action of gradient magnetic force in the direction perpendicular to the cell culture substrate, and effective puncture breakthrough on cell membranes is achieved.