The invention discloses probiotic drops for improving infantile diarrhea and a preparation method of the probiotic drops, and belongs to the technical field of medical biology. The drops comprise thefollowing raw materials of bifidobacterium animalis, bifidobacterium lactis, bifidobacterium breve, lactobacillus rhamnosus, lactobacillus reuteri, lactobacillus acidophilus, lactobacillus fermentum,galactooligosaccharide, xylooligosaccharide, resistant dextrin, alginic acid, natural vitamin A, natural vitamin D, natural vitamin E, unsaturated fatty acid, vegetable oil and phospholipid oil. The preparation method of the drops is simple, and the prepared drops are stable in performance, uniform in component, good in oxidation resistance, more stable in storage and rapid in effect taking.