151 results about "Bio engineering" patented technology

A federated distributed computational system enables secure, privacy-preserving biological data analysis and engineering through interconnected nodes coordinated in a distributed graph architecture. A federation manager allocates resources, manages data flow and lineage, establishes privacy boundaries, and maintains cross-institutional knowledge relationships. Each node contains a processing unit for biological data analysis, privacy preservation protocols for secure multi-party computation, a knowledge graph structure with supporting data stores, and encrypted network connections. The federation manager enforces all computation and data exchange through secure channels while maintaining privacy, security, and contractual boundaries. This architecture enables research institutions to collaborate on complex biological analyses without compromising sensitive data, facilitating breakthrough discoveries through shared computational resources while maintaining strict data privacy and security controls.

The invention belongs to the technical field of bioengineering, and discloses a recombinant yarrowia lipolytica capable of efficiently and rapidly producing sclareol, which is characterized in that optimized sclareol synthase related coding genes ScLPPS, ScSCS, GGPPS1, GGPPS2, ERG20 and tHMGR coding genes are inserted into a yarrowia lipolytica genome; inserting optimized pentadienol utilization pathway coding genes such as IPK, THIM and IDI coding genes; and inserting an optimized ATP: citrate lyase coding gene ACLY to obtain the ATP: citrate lyase coding gene ACLY. The recombinant yarrowia lipolytica is based on knockout of a yarrowia lipolytica strain responsible for coding a non-homologous recombination gene ku70, so that the homologous recombination capability of the yarrowia lipolytica strain is enhanced, gene integration is realized through the homologous recombination function of the yarrowia lipolytica, the genetic stability of the introduced gene can be greatly improved, and the recombinant yarrowia lipolytica strain has a good application prospect. The construction method of the recombinant yarrowia lipolytica strain is efficient and simple to operate.

The invention belongs to the technical field of bioengineering and the technical field of food engineering, and discloses a regulation and control method for improving the DHA content and the sn-2 DHA proportion in schizochytrium limacinum grease fermentation liquor, schizochytrium limacinum algae powder and application of the schizochytrium limacinum algae powder. In the fermentation culture process, the pH of fermentation liquor in the fermentation process is controlled by supplementing acid or alkali in stages. According to the method disclosed by the invention, the pH value of the fermentation liquor is regulated and controlled in stages in the fermentation culture process of the schizochytrium limacinum, and the schizochytrium limacinum can obtain a higher splitting propagation speed, a longer grease accumulation period and a shorter grease consumption end period by regulating and controlling the pH value in the fermentation process. According to the obtained schizochytrium limacinum grease, the oil yield is obviously improved compared with that of a traditional fermentation strategy, and the DHA content and the sn-2 DHA proportion in the oil are also obviously improved.

The invention provides a fluorescent protein selection marker staygold-ynaMr gene and application thereof, and belongs to the technical field of biological engineering. The method comprises the following steps: replacing codons of L-isoleucine in nucleotide sequences of a fluorescent protein gene staygold and a gene ynaM by using a rare codon ATA to respectively obtain the fluorescent protein gene staygold after codon replacement and the gene ynaMr after codon replacement, and connecting the two segments of genes after codon replacement by using a flexible protein peptide, and the fluorescent protein selection marker staygold-ynaMr gene is obtained. Experiments prove that the gene can obviously improve the screening efficiency of L-isoleucine high-yield strains.

The invention relates to the technical field of bioengineering fermentation, and discloses a bioengineering fermentation method, through multiple groups of sensors arranged in a fermentation reactor, key data parameters in the fermentation reactor can be detected and monitored in real time, an operator can be helped to quickly and accurately know the fermentation state of a material in the fermentation reactor, and the fermentation efficiency of the material in the fermentation reactor is improved. Real-time and reliable working parameter adjustment and control are achieved, a multi-parameter dynamic optimization type biological engineering fermentation method is adopted, key parameters in fermentation engineering are accurately analyzed and predicted through dynamic parameter collection in cooperation with a mathematical model and an algorithm, prediction is accurate and reliable, reliable data support can be provided for dynamic regulation and control, and the method is suitable for large-scale popularization and application. The fermentation conditions are dynamically adjusted, so that the growth and metabolism of microorganisms are optimized, the yield and quality of products are improved, the fermentation period is shortened, the production cost is reduced, reliable experience and data support can be provided for subsequent fermentation work in cooperation with data recording, and the fermentation efficiency and the product quality are further optimized.

The invention discloses a method for improving citrus canker resistance through a CsAHL15 gene and application of the CsAHL15 gene, and relates to the field of agricultural biological genes. A method for improving citrus canker resistance through CsAHL15 comprises the following steps that 1, a citrus CsAHL15 coding sequence is cloned, and the CsAHL15 coding sequence is shown as SEQ ID No.1; (2) constructing a CsAHL15 overexpression vector; and (3) CsAHL15 overexpression vector transformation citrus, and identification is carried out to obtain a transgenic plant with improved canker resistance. According to the invention, the citrus CsAHL15 coding sequence is cloned, the overexpression vector is constructed, and then the citrus is transformed, so that the canker attack degree of the obtained transgenic plant can be maximally reduced to 40.09% of that of the existing citrus, the canker attack degree can be obviously reduced, and the scab area can be reduced. In conclusion, the method is a potential biological engineering technology for improving the citrus canker resistance, and has a great value for citrus canker resistance molecular breeding.

The invention relates to the field of biological engineering and technology, in particular to an enzyme for synthesizing glabridin, a biological synthesis method of glabridin and a bacterial strain. The invention discloses a series of glabridin synthesizing enzymes (phenylalanine ammonialyase, cinnamic acid-4-hydrogenase, tyrosine ammonialyase, p-coumaric acid coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4 '-oxymethyltransferase, 4'-hydroxymethyltransferase and 4 '-hydroxymethyltransferase) derived from glycyrrhiza glabra for the first time. ) can be used in the preparation of a compound (e.g., isoflavone 2 '-hydrogenase, isoflavone reductase, Vissisoketoreductase, isoprenyltransferase, pterocarpin reductase, oxidative cyclase, demethylase, cytochrome P450 reductase, and the like). The de novo synthesis of the glabridin in a microbial cell factory is realized for the first time, and the application prospect of the glabridin biological manufacturing industry is greatly promoted.

The invention provides a genetically engineered bacterium and a construction method of the genetically engineered bacterium, and the genetically engineered bacterium is characterized in that a green fluorescent protein sfGFP is used for mediating protein glutaminase, PG (protein glutaminase), PGF (protein glutaminase), PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF, PGF and PGF; eC 3.5. 1.44) is efficiently secreted and expressed in engineering bacteria, and belongs to the technical field of biological engineering. The method comprises the following steps: an obtained sfGFP gene is derived from an NCBI database (GenBank numbering: CP035486.1), an obtained PG zymogen gene PP (Propeptide-Protein glutaminase) is derived from a Chryseobacterium prion prgA gene (GenBank numbering: AB046594.1) in the NCBI database, a recombinant plasmid pHT01 / sfGFP-PP is introduced into bacillus subtilis WB800N to obtain an engineering bacterium, extracellular secretion expression of PP is realized, the sfGFP-PP is activated by trypsin to obtain PG, and the PG is subjected to enzyme activation to obtain the recombinant bacillus subtilis. The expression quantity and the secretion efficiency of the PG in the bacillus subtilis are greatly improved. The recombinant PG produced by the method can generate 23.5 U / mL enzymatic activity under activation of trypsin, can be used for improving protein functional characteristics, and has relatively high industrial production and application values.

The invention relates to the field of biological engineering and technology, in particular to an enzyme for synthesizing glabridin, a biological synthesis method of glabridin and an engineering-modified biological host. The invention discloses a series of glabridin synthesizing enzymes (4 '-O-methyltransferase, isoflavone 2'-hydrogenase, isoflavone reductase, Vissisoketoreductase, isoprenyltransferase, pterocarpin reductase, oxidative cyclase and demethylase) from glycyrrhiza glabra for the first time, and the glabridin synthesizing enzymes have corresponding activities in various biological hosts. The heterologous biosynthesis of the glabridin is realized for the first time, and the yield of the glabridin can reach 60% or above.

The invention provides application of acidophilous glycosyltransferase in salidroside production, and belongs to the technical field of biological engineering. The problem of producing salidroside under the acidic condition is solved. Comprising an application of acidophilous glycosyl transferase with an amino acid sequence as shown in SEQ ID NO.1 in salidroside production under an acidic condition and an acidophilous escherichia coli engineering strain for producing salidroside. The escherichia coli engineering strain overexpresses a mutant 3-deoxy-D-arabinoheptulose-7-phosphate (DAHP) synthetase gene aroGfbr, a cyclohexadiene dehydrogenase gene tyrC, a glucose phosphate mutant enzyme gene pgm and a UDP-glucose pyrophosphorylase galU, overexpresses a phenylpyruvate decarboxylase gene ARO10 derived from saccharomyces cerevisiae, and can be used for producing a mutant 3-deoxy-D-arabinoheptulose-7-phosphate mutant enzyme. The kit comprises an ethanol dehydrogenase gene ADH6 and a glycosyl transferase gene LrUGT85AF8. The method is mainly used for producing salidroside under an acidic condition.

The invention discloses a recombinant escherichia coli engineering strain for producing levodopa and application, and belongs to the technical field of genetic engineering and bioengineering. Escherichia coli WSH-Z06 is used as a starting strain, a levodopa synthesis route is constructed, and supply of cofactors is enhanced by introducing glucose dehydrogenase BmgdH; and the catalytic efficiency of the HpaB is greatly improved by modifying the entrance of the HpaB substrate tunnel. In addition, by optimizing the fermentation pH and induction time, the accumulation amount of the levodopa in the fermentation liquor reaches 60.7 g / L, the production intensity reaches 1.26 g / L / h, a foundation is laid for industrial production of the levodopa, and potential value and significance are achieved for development of synthetic biology.

The invention discloses a schizochytrium limacinum strain for efficiently converting kitchen waste to produce single-cell protein and application of the schizochytrium limacinum strain, and belongs to the technical field of biological engineering. The preservation number of the schizochytrium sp LOX-2 is CGMCC (China General Microbiological Culture Collection Center) No. 41623. The invention also discloses a method for producing single-cell protein by efficiently converting kitchen waste. The method comprises the following steps: fermenting food waste to produce volatile fatty acid; and culturing schizochytrium limacinum by using the strain as a carbon source to obtain single-cell protein. According to the recombinant strain spLOX-2, the utilization of lactic acid is improved, the biomass and the protein yield of the recombinant strain spLOX-2 are respectively improved by 14.19% and 12% compared with those of a wild strain, and the grease content is obviously improved by 107.1%. According to the invention, a new way is provided for a sustainable biological economic mode, wastes are converted into valuable biological chemicals, and a solid foundation is laid for expanding the scale of the process and the economic feasibility of the process in industrial application.

The application discloses a fusion protein NGF2 with improved in-vivo half-life and application thereof, and belongs to the technical field of medical biological engineering, and comprises ABD and FGF2, and the gene and protein sequence thereof are respectively composed of the sequences shown in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:6 and SEQ ID NO:7, and the complete gene and protein sequence thereof is shown in SEQ ID NO:4 and SEQ ID NO:8. The fusion protein NGF2 can keep activity for more than 7 days in an environment of 37 DEG C, and the half-life of the fusion protein in solution is prolonged. In addition, the ABD is combined with HAS to form a complex, and the total molecular weight reaches 90kDa, which is higher than the cut-off molecular weight of glomerular filtration, so that the in-vivo half-life of the fusion protein is effectively prolonged.

The utility model relates to an automatic sampling system of a culture tank. Through accurate control of the control system, automation of the sampling process is achieved, manual intervention is not needed, the sampling efficiency is greatly improved, and the labor intensity is reduced. By utilizing the high-precision sensing device and the control system, the sampling time and the sampling amount can be accurately controlled, the accuracy and the consistency of each sampling are ensured, and the reliability of experimental data is improved. Due to the application of the sterile air driving mechanism and the one-way valve, external pollutants are effectively prevented from being introduced in the sampling process, the purity of a culture environment is guaranteed, and the pollution risk is reduced. By monitoring parameters such as pressure in the culture tank in real time and feeding data back to the control system, the system can intelligently regulate and control the sampling process, and smooth sampling operation is ensured. Through the automatic, high-precision and pollution-free design, the sampling efficiency and accuracy in the cell culture and fermentation process in the field of bioengineering are remarkably improved.

The invention discloses a complex, a capped RNA co-transcription synthesis method, a transcription reaction system and a kit, and relates to the technical field of chemical and biological engineering. Compared with the prior art, the method has the advantages that under the same NTP and cap analogue concentration condition, the mRNA obtained through transcription has a higher cap adding rate, and the high cap adding rate of a wider-spectrum cap analogue can be met. Especially for transcription of cap analogues with complex structures, the method has high cap adding rate and wide application prospects.

The invention belongs to the technical field of biological engineering, and discloses a schizochytrium limacinum engineering strain co-expressing a PDC-ACC gene, a construction method and application, and the genetic engineering strain is obtained by taking schizochytrium limacinum as an original strain and expressing a pyruvate decarboxylase compound gene PDC and an acetyl-coenzyme A carboxylase gene ACC in yarrowia lipolytica. The PDC and ACC genes in the schizochytrium limacinum genetic engineering strain disclosed by the invention are cloned to yarrowia lipolytica, namely Po1f. The yield of traditional fermented grease is 43.90 g / L. The engineering strain performs overexpression on PDC and ACC genes, so that the grease content of the schizochytrium limacinum engineering strain is increased by 53.13% and reaches 67.22 g / L, and the DHA content is increased by 36.97% and is increased from 40.71% to 55.76%.

The invention relates to the technical field of bioengineering fermentation, and discloses a method for synergistically fermenting low-salt raw thick broad-bean sauce by fusing Weissella and Candida angustifolia, and the Weissella and Candida angustifolia are inoculated and fused to strengthen the fermentation function of the low-salt raw thick broad-bean sauce. The salinity of the low-salt raw thick broad-bean sauce prepared by fermentation with the method can be reduced to 8-10%, titratable acid is well controlled, and the color, flavor and typicality of the thick broad-bean sauce are all improved.

The invention discloses construction and application of a recombinant escherichia coli engineering strain with high yield of D-pantothenic acid, and belongs to the technical field of genetic engineering and biological engineering. According to the invention, acetic acid branch pathway genes are knocked out, so that accumulation of precursor pyruvic acid is improved; carrying out free expression and integrated expression on panBC respectively; by weakening a cofactor 5, 10-CH2-THF degradation pathway and strengthening an de novo synthesis pathway of the cofactor 5, 10-CH2-THF, supply of 5, 10-CH2-THF is promoted, and the yield of the constructed engineering strain D-pantothenic acid can reach 4.83 g / L. The Escherichia coli recombinant strain with high yield of D-pantothenic acid, constructed by the invention, lays a foundation for subsequent biosynthesis of D-pantothenic acid and derivatives, and has potential value and significance for development of synthetic biology.

The invention provides recombinant escherichia coli for preparing soluble EC-SOD and application of the recombinant escherichia coli, and relates to the technical field of biological engineering. According to the present invention, SOD and KGF2 genes are connected by using a connecting peptide coding sequence containing a protease cleavage site, and are cloned to a pET28a expression vector; the pET28a expression vector is transformed into competent cells of escherichia coli BL21 (DE3), and recombinant escherichia coli is constructed. The obtained recombinant Escherichia coli can solve the problem that an inactive inclusion body is formed when EC-SOD is expressed in Escherichia coli. The method is suitable for preparing soluble EC-SOD.

The invention relates to the technical field of biological engineering, and particularly discloses an integrated cell treatment super-clean bench, which comprises a super-clean bench body with a fan system, and a pipetting mechanism, an uncovering mechanism, a centrifugal mechanism and a pipe cap opener which are arranged in the super-clean bench body, the super clean bench further comprises a first feeding and discharging mechanism and a second feeding and discharging mechanism, the first feeding and discharging mechanism is used for feeding / discharging culture dishes into / out of the super clean bench body through the feeding and discharging channel, and the second feeding and discharging mechanism is used for feeding / discharging test tubes into / out of the super clean bench body through the feeding and discharging channel. According to the scheme, the problem that a culture dish storage area and a test tube storage area are arranged in the super clean bench and occupy a large space in integrated equipment is solved.

The present invention belongs to the field of biotechnology and its applications, and discloses an application of a transcriptional regulator Cgl2415 in improving the production of ergothioneine in Corynebacterium glutamicum and a method for preparing ergothioneine. The present invention enhances the expression of the transcriptional regulator Cgl2415 , and the enhancement methods include plasmid overexpression, increasing the genomic expression copy number, replacing the strong regulatory element, etc., so as to obtain an increase in the production of ergothioneine. The nucleotide sequence of the transcriptional regulator Cgl2415 is as shown in SEQ ID NO. 2. The method for improving the target product ergothioneine by changing the expression level of the transcriptional regulator of the present invention has guiding significance for the metabolic engineering synthesis and transformation of industrial strains.

The invention discloses a method for preparing the ginsenoside preparation by biological engineering technology, wherein the ginsenoside preparation comprises at least one of the saponin-enriched preparation and the saponin-enriched syrup; the compound bio-enzyme preparation prepared by the combined bacteria preparation of yeast and lactic acid bacteria provided by the invention is rich in amino acid residues, amides, coenzymes and active groups such as lactic acid and linoleic acid; and the ginsenoside preparations containing ginsenosides can be prepared by adding monosaccharides or polysaccharides to the compound bio-enzyme preparations, providing new directions and ideas for obtaining ginsenosides and their products.

The invention belongs to the technical field of biological engineering, and particularly relates to a construction method and application of yeast engineering bacteria for producing 1-aminocyclopropane-1-carboxylic acid (ACC). Pyruvate carboxylase gene RoPYC, yeast SAM enzyme gene Sam2 and ACS synthase gene GmACS are overexpressed in saccharomyces cerevisiae, so that the 1-aminocyclopropane-1-carboxylic acid (ACC) is obtained; a path for biosynthesizing 1-aminocyclopropane-1-carboxylic acid (ACC) by taking glucose as a substrate is designed, a saccharomyces cerevisiae engineering bacterium for producing ACC is constructed, the ACC can be produced by large-scale fermentation, and the highest yield can reach 50.56 g / L. The method has the advantages of low environmental influence, easiness in large-scale production expansion, high production efficiency and the like, has a good economic prospect, and lays a foundation for artificial efficient biosynthesis of ACC.

The invention discloses an AMP sulfating enzyme mutant for producing adenosine phosphoryl sulfuric acid and application, and belongs to the technical field of biological engineering. According to the invention, AMP is used as a raw material, and AMP sulfating enzymes BtaAPSST M2 and PcAPSK are used for biosynthesis of PAPS. Under the catalysis of 10 g / L of BtaAPSST M2 wet thallus and 20 g / L of PcAPSK wet thallus, the conversion rate of PAPS is 70.59%. Compared with the existing ATP sulfating enzyme capable of catalyzing ATP to generate APS, the ATP sulfating enzyme disclosed by the invention is lower in cost and equivalent in conversion rate. Wherein the PAPS yield of the BtaAPSST M2 is two times that of a wild enzyme, and the industrial process of producing the PAPS by a microbial synthesis method is accelerated.

The utility model relates to biological engineering equipment technical field, concretely relates to a biochemical incubator, including base, is equipped with the box on the base, is fixed in the box incubator, is pasted with the conduction sheet on the incubator, and the semiconductor refrigeration heating module is connected with the conduction sheet, and one side of the box is equipped with air filtration system, and is communicated with the incubator through the circulation pipe, and the ultrasonic humidifier is equipped below the semiconductor refrigeration heating module, and is communicated with the incubator through the pipeline, and the incubator is equipped with the sealed door, and there is the gap between the incubator and the box and forms the heat insulation layer, and the controller is connected with the temperature and humidity sensor group, gas sensor group electric signal, can automatically regulate and control each module according to real -time data.

The invention relates to the technical field of bioengineering, in particular to a production strain for synthesizing acetoin by using xylose and application of the production strain. According to the production strain, cellulose hydrolysate is used as a substrate; the production strain is halomonas for enhancing at least one protein function in the following (1) and at least one protein function in the following (2); (1) XYLA and XYLB; and (2) ALS and ALDC. According to the invention, the specific protein function of the halomonas is enhanced by utilizing a biological means, the halomonas synthesis of acetoin by taking cellulose hydrolysate as a substrate is realized, and a new way is provided for industrial production of acetoin.

The invention relates to the technical field of food processing equipment, in particular to a microbial engineering fermentation device.The microbial engineering fermentation device comprises a heater, a water supply heating assembly, an integrated tank assembly and a fermentation liquor circulating assembly.The integrated tank assembly comprises a tank body, a tank body end socket, a rice inlet pipe, an air return pipe, a conical barrel, a water inlet pipe, a high-pressure component and an overflow component; the rice inlet pipe and the air return pipe are fixedly communicated with the rice conveyor, rice is guided into the tank body, the water inlet pipe is communicated with the water supply heating assembly, hot water is input into the tank body, and the high-pressure component introduces high-pressure gas into the tank body from the bottom, so that the rice in the tank body is fully turned over, impurities are washed away, and the rice is discharged from the overflow component. The fermentation liquid circulating assembly enables materials in the tank body to keep balanced fermentation conditions in different areas, fermentation parameters can be accurately set and adjusted, the fermentation period is shortened, the fermentation efficiency is improved, and balanced management integrating cleaning, soaking and fermentation is achieved.

This invention presents a novel process for producing a bio-engineered black TiO2 (B-TiO2) photocatalyst, leveraging the eco-friendly and efficient attributes of bio-synthesis. Distinctively, this method utilizes a natural plant extract as a chelating agent, coupled with deionized water and Titanium(IV) bis / ammonium lactate dihydroxide as the titanium source, eliminating the need for additional pH control compounds, catalysts, or vacuum processes. The process entails forming a precipitate from these components, which is then dried and annealed to produce a black TiO2 powder. This innovative approach not only simplifies the synthesis of B-TiO2 under mild conditions but also enhances its catalytic activity in dark conditions, marking a significant advancement in the field of photocatalysis. The bio-engineered B-TiO2 demonstrates bandgap tunability and a potential for various environmental and industrial applications, highlighting its versatility and the green synthesis method's contribution to sustainable technologies.

The invention discloses application of a corn Zm00001d028750 gene in regulation and control of grain traits, and relates to the technical field of bioengineering, a nucleotide sequence of a coding region of the Zm00001d028750 gene is as shown in SEQ ID NO.1, and an amino acid sequence of a protein coded by the Zm00001d028750 gene is as shown in SEQ ID NO.2. The invention further discloses application of the corn Zm00001d028750 gene in regulation and control of grain traits and application of the corn Zm00001d028750 gene in regulation and control of grain traits. According to the application of the corn Zm00001d028750 gene in regulation and control of grain traits, the hundred-grain weight of corn is an important factor influencing the yield of corn, and along with the increase of the hundred-grain weight of corn, the higher the seed yield is, the higher the yield is; lysine is an essential amino acid for human beings and monogastric animals, and the eating and feeding values of corn can be remarkably improved by increasing the content of lysine and total amino acids.