65 results about "Bio engineering" patented technology

The application discloses a fusion protein NGF2 with improved in-vivo half-life and application thereof, and belongs to the technical field of medical biological engineering, and comprises ABD and FGF2, and the gene and protein sequence thereof are respectively composed of the sequences shown in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:6 and SEQ ID NO:7, and the complete gene and protein sequence thereof is shown in SEQ ID NO:4 and SEQ ID NO:8. The fusion protein NGF2 can keep activity for more than 7 days in an environment of 37 DEG C, and the half-life of the fusion protein in solution is prolonged. In addition, the ABD is combined with HAS to form a complex, and the total molecular weight reaches 90kDa, which is higher than the cut-off molecular weight of glomerular filtration, so that the in-vivo half-life of the fusion protein is effectively prolonged.

The invention discloses a method for preparing the ginsenoside preparation by biological engineering technology, wherein the ginsenoside preparation comprises at least one of the saponin-enriched preparation and the saponin-enriched syrup; the compound bio-enzyme preparation prepared by the combined bacteria preparation of yeast and lactic acid bacteria provided by the invention is rich in amino acid residues, amides, coenzymes and active groups such as lactic acid and linoleic acid; and the ginsenoside preparations containing ginsenosides can be prepared by adding monosaccharides or polysaccharides to the compound bio-enzyme preparations, providing new directions and ideas for obtaining ginsenosides and their products.

The utility model relates to biological engineering equipment technical field, concretely relates to a biochemical incubator, including base, is equipped with the box on the base, is fixed in the box incubator, is pasted with the conduction sheet on the incubator, and the semiconductor refrigeration heating module is connected with the conduction sheet, and one side of the box is equipped with air filtration system, and is communicated with the incubator through the circulation pipe, and the ultrasonic humidifier is equipped below the semiconductor refrigeration heating module, and is communicated with the incubator through the pipeline, and the incubator is equipped with the sealed door, and there is the gap between the incubator and the box and forms the heat insulation layer, and the controller is connected with the temperature and humidity sensor group, gas sensor group electric signal, can automatically regulate and control each module according to real -time data.

The invention relates to the technical field of food processing equipment, in particular to a microbial engineering fermentation device.The microbial engineering fermentation device comprises a heater, a water supply heating assembly, an integrated tank assembly and a fermentation liquor circulating assembly.The integrated tank assembly comprises a tank body, a tank body end socket, a rice inlet pipe, an air return pipe, a conical barrel, a water inlet pipe, a high-pressure component and an overflow component; the rice inlet pipe and the air return pipe are fixedly communicated with the rice conveyor, rice is guided into the tank body, the water inlet pipe is communicated with the water supply heating assembly, hot water is input into the tank body, and the high-pressure component introduces high-pressure gas into the tank body from the bottom, so that the rice in the tank body is fully turned over, impurities are washed away, and the rice is discharged from the overflow component. The fermentation liquid circulating assembly enables materials in the tank body to keep balanced fermentation conditions in different areas, fermentation parameters can be accurately set and adjusted, the fermentation period is shortened, the fermentation efficiency is improved, and balanced management integrating cleaning, soaking and fermentation is achieved.

This invention presents a novel process for producing a bio-engineered black TiO2 (B-TiO2) photocatalyst, leveraging the eco-friendly and efficient attributes of bio-synthesis. Distinctively, this method utilizes a natural plant extract as a chelating agent, coupled with deionized water and Titanium(IV) bis / ammonium lactate dihydroxide as the titanium source, eliminating the need for additional pH control compounds, catalysts, or vacuum processes. The process entails forming a precipitate from these components, which is then dried and annealed to produce a black TiO2 powder. This innovative approach not only simplifies the synthesis of B-TiO2 under mild conditions but also enhances its catalytic activity in dark conditions, marking a significant advancement in the field of photocatalysis. The bio-engineered B-TiO2 demonstrates bandgap tunability and a potential for various environmental and industrial applications, highlighting its versatility and the green synthesis method's contribution to sustainable technologies.

The invention discloses application of a corn Zm00001d028750 gene in regulation and control of grain traits, and relates to the technical field of bioengineering, a nucleotide sequence of a coding region of the Zm00001d028750 gene is as shown in SEQ ID NO.1, and an amino acid sequence of a protein coded by the Zm00001d028750 gene is as shown in SEQ ID NO.2. The invention further discloses application of the corn Zm00001d028750 gene in regulation and control of grain traits and application of the corn Zm00001d028750 gene in regulation and control of grain traits. According to the application of the corn Zm00001d028750 gene in regulation and control of grain traits, the hundred-grain weight of corn is an important factor influencing the yield of corn, and along with the increase of the hundred-grain weight of corn, the higher the seed yield is, the higher the yield is; lysine is an essential amino acid for human beings and monogastric animals, and the eating and feeding values of corn can be remarkably improved by increasing the content of lysine and total amino acids.

The invention discloses issatchenkia orientalis capable of efficiently synthesizing L-malic acid and application of the issatchenkia orientalis, and belongs to the technical field of biological engineering. In an existing gene edited yeast strain in a laboratory, endogenous lactic dehydrogenase IoLDH2 and IoLDH3 are over-expressed, endogenous IoLDH1 is knocked out, and lactic dehydrogenase EcD-LDH from escherichia coli and lactic dehydrogenase NmD-LDH from neisseria meningitidis are over-expressed. After fermentation for 72 h in a 5 L fermentation tank, the accumulation amount of L-malic acid reaches 296.27 g / L, the yield is 0.97 g / g, the production intensity is 4.11 g / L / h, and the by-product lactic acid is remarkably reduced from 23.56 g / L to 6.7 g / L and is reduced by 71.56%.

The invention discloses photoresponse hydrogel as well as a preparation method and application thereof, and belongs to the technical field of intelligent materials. The preparation method specifically comprises the following steps: (1) synthesizing a compound 1; (2) synthesizing a compound 2; (3) dissolving acryloyl chloride in dichloromethane, dropwise adding into a dichloromethane mixed solution containing the compound 2 and triethylamine, stirring, washing, concentrating under reduced pressure, and recrystallizing; and (4) dissolving N, N-dimethylacrylamide, a phenylboronic acid monomer, diol, MEH and N, N '-methylene bisacrylamide in water, filling nitrogen, adding azodiisobutyronitrile, gelatinizing, and dialyzing, so as to obtain the hydrogel. The hydrogel based on visible light driven deformation is designed and prepared, controllable change of shape, volume or motion can be achieved through illumination, and the core application of the hydrogel comprises soft robot application, medical and biological engineering application, environment and energy application and other innovative application.

The invention belongs to the technical field of molecular biology, and particularly discloses a method for improving citrus canker resistance by using a CsENPP1 gene, the coding protein of the CsENPP1 gene is citrus nucleotide pyrophosphatase / phosphodiesterase 1, the coding sequence of the CsENPP1 is a nucleotide sequence shown as SEQ ID No.1, and the citrus canker resistance is improved based on overexpression of the CsENPP1 gene in citrus cells. The citrus nucleotide pyrophosphatase / phosphodiesterase 1 coding gene is integrated into citrus through an expression vector, the canker attack degree of the obtained transgenic material can be reduced to 48.9% of that of the existing citrus to the maximum extent, the canker attack degree can be remarkably reduced, the scab area is reduced, and the yield of the citrus is improved. The technology provided by the invention is a bioengineering technology which has potential to improve the citrus canker resistance, and has great value for citrus canker resistance molecular breeding.

The invention relates to the technical field of biological engineering, in particular to application of a gene ZmNPF6.8 in regulation and control of corn nitrogen absorption and utilization efficiency and single-plant yield of corn. The gene ZmNPF6.8 has the following characteristics: 1) a coding region nucleotide sequence as shown in SEQ ID NO: 3; and 2) a nucleotide sequence which has more than 90% of homology with the nucleotide sequence as shown in SEQ ID NO: 3 and forms protein with the same function as the nucleotide sequence as the nucleotide sequence as shown in SEQ ID NO: 3. According to the invention, the expression quantity of the gene ZmNPF6.8 can be down-regulated by inserting a Mutuator transposon into an amino acid coding region of the gene, and meanwhile, the nitrogen absorption and utilization efficiency and the yield per plant of corn are remarkably reduced. Researches show that the nitrogen absorption and utilization efficiency of the corn and the change of the yield of a single plant of the corn can be regulated and controlled by changing the expression quantity of the gene ZmNPF6.8, and the gene is of great significance to the high-efficiency and high-yield breeding work of the corn in the future.

The application discloses a tissue culture regeneration system and culture method of Hunan millet, and relates to the field of biological engineering. The regeneration system is to take the Hunan millet seeds with removed hulls as explants, and utilizes a culture medium composed of 1 / 2MS basic culture medium, 0.6-0.99 mg / L 2,4-D, 30 g / L sucrose, 7 g / L agar and 0.1% PPM, with pH being 5.8, to make the explants form complete Hunan millet plants. The regeneration system is used for tissue culture of the Hunan millet, effectively avoiding the problems of long culture period, high requirement for personnel and high culture cost caused by diversification of culture medium configuration in large-scale factory production. The application provides a simpler and more efficient method for large-scale factory production, research on gene function and molecular breeding and the like.

The invention belongs to the field of bioengineering, and particularly relates to a construction method and application of a transgenic mouse for screening tissue cell membrane protein in vivo. Aiming at the problems of low in-vivo research flux, unstable library, limited tissue applicability and the like of the traditional membrane protein gene, sgRNA library plasmids are constructed by designing sgRNA of a membrane protein target gene and a control gene; three-positive-mouse containing Cas9, UBC-CreERT2 and sgRNA libraries is obtained through embryo microinjection and hybridization, genotype identification, inducer regulation and control, disease modeling, multi-tissue sample treatment and NGS analysis technical systems are established in a matched mode, and membrane protein gene in-vivo high-throughput screening is achieved. The model can guarantee library stability and screening reliability, covers multi-tissue research scenes, links in-vitro screening and in-vivo verification and is suitable for membrane protein function analysis and disease target mining, the technical process can be popularized to other gene families, and systematic biological research is promoted.

The invention discloses a method for constructing a nasal mucosa epithelium single-layer model by using a single-cell digested organoid, and belongs to the technical field of bioengineering. The method comprises the following steps: firstly, carrying out digestion treatment, red blood cell lysis treatment, inoculated culture and passage on nasal mucosa tissues to obtain nasal mucosa organoid, then adding recombinase for dissociation to obtain dissociated epithelial cells, finally, planting the dissociated epithelial cells in a culture container subjected to coating treatment for incubation and adherence, and continuing to culture to obtain the nasal mucosa organoid. And preparing the nasal mucosa epithelium single-layer model. The multi-lineage differentiation potential and individual specificity of the organoid-derived cells are reserved, the cell adherence, passage and cryopreservation recovery stability is remarkably improved, the culture period is shortened, reagent expenditure and time cost are saved, and the defects that a traditional cell line is poor in physiological correlation, difficult in primary and gas-liquid interface culture acquisition, long in period and low in cost are overcome. And the three-dimensional organ-like chamber structure is not beneficial to top surface observation and solute transport research, so that the three-dimensional organ-like chamber structure is more suitable for scientific experiments.

The invention discloses a method for synthesizing levosalbutamol and a precursor thereof through three-enzyme cascade catalysis, and belongs to the technical field of biological engineering. The invention designs a new route for efficiently biologically synthesizing the levosalbutamol and the precursor thereof by taking cheap 5-[2-[(1, 1-dimethylethyl) amino] acetyl]-2-hydroxybenzaldehyde as a substrate through three-enzyme cascade catalysis of benzyl alcohol dehydrogenase, carbonyl reductase and glucose dehydrogenase. When the substrate concentration is 100 mM, the yield of the levosalbutamol reaches 98%, and the enantiomeric excess (ee) is greater than 99%. In addition, according to the route, 5-bromoacetyl-2-hydroxybenzaldehyde can be used as a substrate, and the levosalbutamol precursor can be efficiently synthesized through three-enzyme cascade catalysis. Compared with the existing method for mainly producing the levosalbutamol and the precursor thereof, the method disclosed by the invention has the advantages that the production cost is obviously reduced, the synthesis efficiency and the chiral purity of the product are improved, and the method has a wide application prospect.

The invention relates to the technical field of bioengineering, and particularly discloses a microorganism 3D printing embedding immobilization method based on double-network hydrogel. A microbial 3D printing embedding immobilization method based on double-network hydrogel comprises the following steps: heating and dissolving polyvinyl alcohol and sodium alginate in water, then adding a nano material, stirring and mixing uniformly, cooling, sequentially adding acrylamide, a cross-linking agent and a microbial agent, and mixing uniformly to obtain a first network premixed solution; dissolving calcium chloride in a saturated boric acid solution to obtain a solidification liquid; filling the first network premixed liquid into an extrusion type 3D printer, and immersing a printing head into the solidification liquid for printing to obtain a gel structure; taking out the gel structure from the solidification liquid, immersing the gel structure into a solution containing an initiator, and initiating polymerization to form stable dual-network hydrogel; and taking out the double-network hydrogel, and cleaning to obtain a final microorganism embedding product.

The invention relates to the field of microbial engineering, in particular to an engineering modified bacillus and a construction method and application thereof. The engineered bacillus comprises a MreB protein fused with a mutated ssrA tag protein at the C-terminal, and the engineered bacillus does not express an SspB protein. The volume of the engineering modified bacillus provided by the embodiment of the invention can be increased to more than 5 times of the volume of a wild type in the later growth period. When the engineering modified bacillus is used for preparing biological products through fermentation, the production efficiency can be remarkably improved.

The invention relates to the field of microbial engineering, in particular to an engineering modified bacillus and a construction method and application thereof. The engineered bacterium comprises an MreB protein fused with a mutated ssrA tag protein at the C-terminal, does not express an SspB protein, and overexpresses a cell division inhibiting gene. The volume of the engineering modified bacillus provided by the embodiment of the invention can be increased to 10 times of that of a wild type in the later growth period. When the engineering modified bacillus is used for preparing biological products through fermentation, the production efficiency can be remarkably improved.

The invention relates to recombinant human VII type collagen as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. According to the invention, a section of VII type collagen sequence with high activity, strong stability and good water solubility is analyzed and screened, the VII type collagen sequence is connected in series to form a section of brand new recombinant human source VII type collagen, and pichia pastoris is selected as a carrier for expressing foreign protein. The nucleotide of the sequence is subjected to pichia pastoris codon preference optimization, and a stable and high-yield recombinant human source VII type collagen gene engineering strain is successfully constructed by an integrated plasmid mediated recombinant bacterium construction method, and the collagen yield can reach 7g / L. The recombinant human VII type collagen prepared by the invention has good activity of promoting cell proliferation and migration, is superior to the existing commercially available products, and can be widely applied to the fields of food, cosmetics, health care products and medical and mechanical products.

The invention relates to the technical field of water and soil conservation and water resource regulation and control, in particular to a soil-biology-engineering reservoir collaborative reservoir expansion and capacity increase method and system for a dry and hot valley area. By constructing a'soil-biology-engineering 'multi-source reservoir cooperative system, full-watershed sediment control, vegetation structure optimization, efficient water utilization and'three-reservoir' cooperative capacity increase are achieved, and the method has the following beneficial effects that (1) reservoir sediment deposition is remarkably reduced, and the engineering service life is prolonged; vegetation survival rate and ecological stability are improved, and manual irrigation dependence is reduced; (3) the utilization efficiency of water resources is improved by 30-50%; 4, the soil water storage capacity is enhanced, and the soil structure is improved; and (5) resource utilization of ineffective rainfall is realized, and regional drought resistance is improved.

The invention relates to neurobiology, molecular virology, biomedical engineering and the like, and discloses a domesticated low-serum Vero cell line and a method for preparing an HSV virus vector through suspension culture cell amplification. Aiming at the bottleneck problem that a process for amplifying HSV by adherent culture of Vero cells is difficult to realize large-scale and engineering amplified production, the invention researches and develops a method for preparing HSV based on suspension culture of cell amplification. Vero cells introduced from ATCC are domesticated, and the serum content of a culture medium is gradually reduced, so that the Vero cells are suitable for suspension culture in a microcarrier or sheet carrier reactor by using a low-serum or serum-free culture medium. The method comprises the following steps: using Tamp of Dibide Bioengineering (Shanghai) Co., Ltd; and carrying out suspension culture of the Vero cells and infection amplification of the HSV virus vector by using a J Cloud Ready TM bioreactor. High-activity suspension culture and HSV amplification of Vero cells are realized by optimizing production conditions, cell density, biochemical detection and the like, and a stable and efficient method is provided for preparing a high-titer and high-purity HSV virus vector in combination with purification process upgrading.

The invention relates to glycosyltransferase, a nucleic acid fragment, a recombinant expression vector, a host cell and application of the glycosyltransferase. The amino acid sequence of the glycosyl transferase is as shown in SEQ ID NO: 1. According to the application, an enzyme for converting 3-oxo-ionol glycosyl into 3-oxo-ionol glucoside is found from tobacco, namely, a novel glycosyl transferase is provided, and the function of the enzyme in biosynthesis of related glycosyl transferase genes is cloned and verified for the first time; in vitro, the recombinant protein can be used for efficiently converting a 3-oxo-ionol substrate into corresponding glycoside. The invention further provides a recombinant plasmid containing the glycosyl transferase gene, glucoside can be massively synthesized through a bioengineering method, meanwhile, the invention further provides application of the recombinant plasmid capable of massively synthesizing 3-oxo-ionol glucoside, more basic elements are provided for synthetic biology of natural products, and the recombinant plasmid can be used for preparing a large amount of 3-oxo-ionol glucoside. In addition, guidance and basis are provided for rational design of the enzymes, and the method has good industrialization prospects.

The invention provides engineering probiotics EcN-Vh as well as a construction method and application thereof, and belongs to the technical field of biological medicines. According to the present invention, the engineering probiotic capable of expressing the BTApep-TAT is constructed through the bioengineering technology, the BTApep-TAT is expressed through the plasmid integration mode, the release of the peptide is achieved by using the arabinose-induced cracking system, and the engineering probiotic is used for the in-vivo sustainable production of the BTApep-TAT. The engineering probiotic EcN-Vh disclosed by the invention can continuously generate an inhibitory peptide BTApep-TAT of a targeted BORIS (Bovine Biosensor), and is used for treating the colorectal cancer. According to the probiotics, by inhibiting ADP-ribosylation and Wnt / beta-catenin signal channels of BORIS, the occurrence rate and progress of tumors are remarkably reduced, and a novel, safe and efficient probiotic delivery system is provided for treatment of colorectal cancer.

The invention discloses a genetically engineered bacterium for catalyzing synthesis of ornamental blue from an ornamental blue derivative as well as a construction method and application of the genetically engineered bacterium, and relates to the technical field of bioengineering. According to the invention, on the basis of a gene editing technology, CobB, NOX, katE, NAMPT, nadD, rbsk, prs and ppk genes are integrated onto an escherichia coli genome; a genetically engineered bacterium for catalyzing synthesis of the observation blue derivative (N-acetylobservation blue or N, N '-diacetyl-observation blue) is constructed, synthesis of NAD + can be enhanced, cyclic regeneration of NAD + can be realized, H2O2 which is generated in the synthesis process of NAD + and has a toxic effect on enzyme is eliminated, supply of cofactors PRPP and ATP is enhanced, deacetylase is activated, and the observation blue derivative (N-acetylobservation blue or N, N'-diacetyl-observation blue) is enabled to be converted into the observation blue derivative (N-acetylobservation blue or N, N '-diacetyl-observation blue) into the observation blue derivative (N-acetylobservation blue or N, N'-diacetyl-observation blue). N, N '-diacetyl-observation blue) is used for catalytic synthesis of observation blue.

The present disclosure belongs to the technical field of biological engineering, and in particular to a temperature-sensitive aroma-producing Saccharomyces cerevisiae and use thereof in preparation of flavor enhancers. The temperature-sensitive aroma-producing Saccharomyces cerevisiae is preserved in China Center for Type Culture Collection on May 31, 2024, with the preservation number of CCTCC NO: M20241116. The present disclosure adopts a mixed fermentation method of temperature-sensitive aroma-producing Saccharomyces cerevisiae and Corynebacterium glutamicum (the preservation number is CCTCC NO: M 2024431) to obtain a fermentation broth containing high concentrations of glutamic acid and nucleotides. Simultaneously, the fermentation broth also contains a variety of functional nutrients, such as other amino acids, organic acids and β-glucan, enabling higher functional nutritional value.

The application belongs to the technical field of microorganisms and particularly relates to a Pichia kudriavzevii and application thereof. Pichia kudriavzevii The Pichia kudriavzevii is Pichia kudriavzevii AMCC 31367 (Pichia kudriavzevii CCTCC NO: M 20251680). The Pichia kudriavzevii has the characteristics of multi-environment tolerance, broad-spectrum substrate applicability and high protein and amino acid content, and can tolerate high-concentration lactic acid, and the yeast can still grow under the lactic acid concentration of 170 g / L, thereby solving the problems of limited raw material selection, low production efficiency and high cost in the development of microbial cell protein, and the strain has application potential in the fields of biological materials, microbial engineering, environmental governance, feed and food processing.

The invention provides a sample data generation method and device, equipment and a storage medium, which can be applied to various scenes such as biological engineering and biological pharmacy. The method comprises the following steps: acquiring sequence data of a first biomolecule and structural data of the first biomolecule and a second biomolecule; determining a group belonging to a binding site in groups included in the structural data of the first biomolecule based on the spatial information of the first biomolecule and the second biomolecule; labeling whether each group included in the sequence data of the first biomolecule belongs to a binding site or not based on the group belonging to the binding site in the groups included in the structural data of the first biomolecule to obtain label information of the first biomolecule; and on the basis of the tag information of the first biomolecule, the sample data corresponding to the first biomolecule is generated, so that the quality of the sample data is improved, the labor cost is low, the efficiency is high, and large-scale and standardized data samples can be obtained.

The invention belongs to the technical field of food science and bioengineering, and provides a method for improving antioxidant and hypoglycemic activity of bovine bone enzymatic hydrolysate. The method comprises the following steps: performing ultrasonic pretreatment on cattle bone powder (500W, 30min), performing alkaline protease hydrolysis (50 DEG C, 5h), mixing the inactivated cattle bone powder with D-xylose (8: 1), and performing Maillard reaction (100 DEG C, 120min). The DPPH free radical scavenging rate and the ABTS free radical scavenging rate of the obtained product respectively reach 91% and 88.13%, and the antioxidant activity is remarkably improved; the synthetic peptide, such as FGFDGDFYR, has both the hydroxyl radical scavenging rate of 74.52% and the alpha-glucamylase inhibition rate of 70.2%, so that the double-function activity of resisting oxidation and reducing blood sugar is realized; meanwhile, the product is enhanced in palatable taste and reduced in bitter taste, is rich in meat flavor compounds (such as 2-methyl-3-furanmercaptan), has flavor and functional advantages, and promotes high-value utilization of bovine bones.

The utility model discloses a biological enzyme purification and separation device, and relates to the technical field of biological engineering. Under the action of the fixing mechanism, the shaking influence generated during purification and separation can be reduced, so that the actual use effect and quality can be ensured; the driver is started to drive the inner cylinder to perform circular motion, bio-enzyme liquid in the inner cylinder is purified and separated through the purification layer, and under the action of centrifugal force, the bio-enzyme liquid flows into the outer cylinder through the through holes in the inner cylinder and enters the crystallization equipment through the liquid discharge pipe and the connecting pipe for crystallization. The high-purity enzyme crystals can be obtained through crystallization of the crystallization equipment, the volume of the purified and filtered bio-enzyme liquid in the outer barrel can be monitored in real time through the liquid level sensor, and when the volume exceeds an early warning value, material injection into the hopper is stopped.

The invention discloses a construction method and application of escherichia coli capable of producing fumaric acid, and belongs to the technical field of biological engineering. By-product synthesis related genes such as a fumarase coding gene fumA, a lactic dehydrogenase coding gene ldhA and a pyruvate oxidase coding gene poxB are knocked out, so that the yield of fumaric acid is effectively increased, and the content of heteroacid is reduced. On the basis, the expression of Afpyc, Ecppc and EcaceA genes is up-regulated independently or in a combined manner. The genetically engineered bacterium constructed by the invention adopts an aerobic-microaerobic two-stage fermentation strategy in a 5L fermentation tank, the highest yield of fumaric acid reaches 50.2 g / L after fermentation for 60 hours, the glucose conversion rate is 0.48 g / g, and the by-products lactic acid and acetic acid are both controlled to be 4-5g / L. The strain does not need to carry plasmids, so that the risks of antibiotic use and plasmid loss are avoided, and the strain has good industrial application potential.