578 results about "Tissue sample" patented technology

The utility model relates to the technical field of fixation and preservation of biological tissue samples, and provides an embedding mold for multiple frozen tissues, which comprises a base, a plurality of clamping pieces, a plurality of clamping pieces and a plurality of clamping pieces, and is characterized in that the top surface is provided with a pair of centrosymmetric supporting blocks; the sleeve is detachably arranged outside the pair of supporting blocks in a sleeving manner; wherein the pair of supporting blocks are arranged at an interval of 180 degrees, and the outer side walls of the supporting blocks are attached to the inner side wall of the sleeve in a transition fit mode; the sleeve is as high as the supporting block, and an accommodating cavity is defined by the inner side wall of the sleeve and is used for embedding the plurality of strip-shaped frozen tissue blocks in a vertical form. The containing cavity formed in the sleeve is changed into a columnar shape with enough depth from an existing flat shape with limited depth, the problems that the tissue structure is incomplete and an OCT embedding medium overflows are directly solved, the edge of a tissue combined block is smooth, slicing is facilitated, meanwhile, a plurality of strip-shaped frozen tissue blocks are embedded in a vertical mode, and the working efficiency is improved. The height of the tissue block is unified, the size of the tissue block is fixed, and the problems that sample loss is large and tissue arrangement is too close to the edge are solved.

The invention relates to the technical field of digital pathology acquisition, and discloses a digital pathology picture acquisition method. The method comprises the following steps: acquiring sample characteristic data such as thickness, dyeing degree and structural characteristics of a tissue sample to be detected, and determining initial image acquisition parameters including exposure time, image resolution and optical magnification; and meanwhile, environment condition data such as temperature, humidity and environment illumination intensity of a scanning environment are collected, so that whether the initial parameters are adjusted or not is judged. When adjustment is needed, collecting preliminary image data, extracting quality features including image definition, color accuracy and detail retention degree, calculating feature values, and comparing the feature values with historical image quality feature values in a historical database; and if the same characteristic value does not exist in the historical database, calculating the similarity, and adjusting the initial image acquisition parameters according to the comparison result or the similarity. According to the method, sample characteristics and environmental conditions are comprehensively considered, and the quality of the digital pathological picture is effectively improved.

A tamper for making paraffin blocks for histology may include a pliable tamper head and a handle. The handle may be permanently or removably attached to the tamper by using screws, magnets, or the like. The tamper may be made from a pliable material, such as a silicone rubber, with improved thermal transfer and high latent heat. In use, the tamper may be warmed in advance to a working temperature by placing the tamper on a hot plate of the embedding station or on a dedicated smaller hot plate. Alternatively, the tamper may be heated with an electric resistive element, a thermoelectric device, or an electric induction coil.

The invention relates to the technical field of bioengineering, in particular to an exosome separation and concentration method with marine animal source tissue as a raw material. Comprising the following steps: freeze-drying and crushing a marine animal source tissue sample; performing sample enzymolysis; and removing impurities and concentrating. According to the method disclosed by the invention, the exosome in the cells is fully dissociated through freeze-dried powder crushing and composite enzymolysis processes, and the filtering speed of the sample is accelerated by virtue of ultrasonic oscillation, so that the rapid and high-purity separation and concentration of the exosome in the marine animal source tissue are realized.

The invention relates to the technical field of biopsy picking devices, and particularly discloses a gastrointestinal endoscope biopsy picking device which comprises a flexible outer tube. The fixing base is fixedly connected with the end part of the flexible outer pipe; the number of the clamp flaps is two, and meshing teeth are arranged on the sides, close to each other, of the two clamp flaps; the opening and closing mechanism penetrates through the flexible outer tube and is connected with the two clamp flaps; according to the gastrointestinal endoscope biopsy extraction device, the expansion mechanism expands through the trigger type air bag, so that the root of a deep plica is expanded outwards, a dead angle area which cannot be expanded through conventional air injection is fully exposed, a doctor does not need to manually control an endoscope to push the plica or adjust the angle, semi-blind operation is not needed, missed diagnosis of a hidden focus is avoided, and the operation efficiency is improved. After the plica is unfolded, the clamp flaps can be vertically attached to a focus mucous membrane, the staggered design of the meshing teeth ensures that a tissue sample is firmly fixed, the focus is automatically exposed through the expansion mechanism, the endoscope view is clear, the operation process is simplified, and the sampling failure rate is reduced.

The invention discloses an angle-adjustable interventional biopsy needle suitable for deep tumors and a sampling method of the angle-adjustable interventional biopsy needle, and relates to the field of tumor interventional biopsy sampling, the angle-adjustable interventional biopsy needle comprises a guide sheathing canal, the guide sheathing canal is composed of an outer sheath layer on the outermost layer, a braid layer in the middle and a first lining layer on the inner layer, and a bending mechanism is arranged at the bottom of the guide sheathing canal; the bending mechanism comprises an active bending tube, the active bending tube is fixedly connected with the bottom of the guiding sheath tube, a protection pad is arranged at the bottom of the active bending tube, and a second lining layer is arranged on the inner side of the active bending tube. On the premise of single puncture, multi-point and multi-angle sampling of different quadrants and different depths in a tumor can be easily achieved, representative tissue samples can be obtained to the maximum extent through the design, sampling errors caused by tumor heterogeneity are effectively avoided, the occurrence rate of false negative results is remarkably reduced, and the sampling efficiency is improved. And a more reliable pathological basis is provided for clinical diagnosis.

Methods include applying a first stain composition comprising a fluorescent counterstain to a biological sample, measuring information corresponding to one or more stains of the first stain composition, removing the fluorescent counterstain from the biological sample, applying a second stain composition to the biological sample, measuring information corresponding to one or more stains of the second stain composition in the biological sample, where the one or more stains include a fluorescent label, and generating a first image of the biological sample, where the first image corresponds to a pattern of simulated hematoxylin staining in the biological sample.

Abstract A method for automated preparation of a regenerative epidermal suspension is disclosed. A cartridge containing a tissue sample, a cup and a well plate having wells containing enzyme solution and buffer solution is positioned within a processing system. In response to an initiation signal, a processor controls operation of a tissue disaggregator to mechanically disaggregate the tissue sample while sequentially exposing the tissue sample to the enzyme solution and the buffer solution. The tissue disaggregator may apply rotational, grinding and / or pressing forces to the tissue sample supported on a screen within the cup. The processor may further control movement of the cup, rotation of the well plate and operation of one or more motors to automate preparation of the regenerative epidermal suspension. Abstract 20 26 20 46 06 1 6 Ju n 20 26 wo 2024 / 145258 PCT / US2023 / 085844 16 Jun 2026 303 301 * 2026204606 300 308 305 00 307 306 309 312 310 314 311 315 313 316 317 321 317a 318 320 319 322 FIG. 3A

This invention provides a feeding mechanism for a biological tissue sample staining device, including a translational feeding assembly, a sample slide feeding lifting assembly, a sample slide feeding heating chamber, and a microscope slide vertical support. The translational feeding assembly has a translational feeding platform capable of holding multiple microscope slide vertical supports. Multiple microscope slides can be vertically fixed in an array within each microscope slide vertical support, with each slide spaced apart. The sample slide feeding lifting assembly cooperates with the translational feeding platform. The sample slide feeding heating chamber is positioned above the sample slide feeding lifting assembly, and the sample slide feeding lifting assembly can reciprocate between the translational feeding platform and the sample slide feeding heating chamber. The beneficial effect of this invention is that it can avoid the risk of cross-contamination between microscope slides.

The invention discloses a low-intensity transcranial focused ultrasound cerebral apoplexy rehabilitation animal experiment method and system. The method sequentially comprises the following steps of ultrasonic parameter safety calibration, animal screening and baseline establishment, cerebral apoplexy animal model preparation and establishment, ultrasonic intervention treatment, neurobehavioral dynamic evaluation, tissue sample acquisition and processing and multi-modal tissue inspection. The system comprises a stimulation unit, a positioning unit and a control unit, and is used for realizing accurate and parameter-controllable ultrasonic intervention. By constructing a complete research closed loop from parameter safety verification and standardized model intervention to multi-dimensional effect evaluation, the problems of blind parameter selection, single evaluation system and nonstandard experimental process in the prior art are solved, and a set of standardized and repeatable research normal form is provided. The rehabilitation effect of the LIFU on the cerebral apoplexy can be comprehensively and objectively evaluated, and parameter optimization can be guided.

A biopsy system includes a biopsy probe configured to sever tissue to acquire a tissue sample. The biopsy probe has biopsy needle portion having a proximal end, a distal end, and a lumen. A vacuum source is coupled to the lumen. The vacuum source is configured to generate a vacuum to transport the tissue sample through the lumen and expel the tissue sample from the lumen at the proximal end of the biopsy needle portion. An arrangement of compression plates is configured to receive the tissue sample from the proximal end of the biopsy probe and is operable to compress the tissue sample. The arrangement of compression plates has a plurality of impedance measurement electrodes. An impedance measurement circuit is connected to the plurality of impedance measurement electrodes.

This application belongs to the field of fluorescence microscopy imaging, and more specifically, relates to a dual-gel system suitable for dilatational microscopy of large-volume biological tissues. This dual-gel system achieves uniform expansion and morphological stability of tissue samples through a dilatational gel, and then imparts high mechanical strength using a stabilizing gel. This allows millimeter-scale large-volume biological tissue samples to possess both high expansion ratio and excellent mechanical stability, achieving 3-5 times linear expansion with a Young's modulus of not less than 300 kPa. It can stably adapt to vibrational cutting operations and support cutting of 100-500 μm thick layers, with intact cut surfaces and good interlayer consistency, greatly improving the efficiency and quality of three-dimensional data acquisition. Furthermore, the hydrogel sections obtained from the cutting have intact morphology and can be used as independent slides for multidimensional analysis. This application overcomes the limitations of traditional dilatational microscopy techniques in terms of sample volume and mechanical stability, providing an efficient and reliable solution for the three-dimensional reconstruction of millimeter-scale biological tissues.

The present disclosure provides methods of amplifying tissue samples derived from humans for purposes of treating certain diseases. The method comprises: isolating a small amount of associated tissue by biopsy or other tissue acquisition methods; the tissue is amplified in vitro, and meanwhile, natural functions are reserved; and re-implanting the amplified tissue to alleviate human disease. One example includes ex vivo expansion of human pancreatic islet tissue and reimplantation of the expanded cells into an individual with Type 1 diabetes for treatment and long term relief or cure of disease.

The invention relates to a method for detecting alkaloid compounds based on UPLC-MSMS (ultra performance liquid chromatography-mass spectrometry) and application. The method comprises the following steps: after a sample is extracted, detecting a sample extracting solution and a standard solution by adopting an ultra performance liquid chromatography-mass spectrometry combined method; a mobile phase adopted by the ultra-high performance liquid chromatography is as follows: a mobile phase A is an aqueous solution containing 0.1% of formic acid; the mobile phase B is an acetonitrile solution containing 0.1% of formic acid. According to the method disclosed by the invention, after the extracted tissue sample is subjected to liquid chromatography separation, the alkaloid metabolites are qualitatively and quantitatively detected in a targeted manner, and due to the high selectivity of the high-resolution mass spectrum, parameter optimization does not need to be performed on each metabolite.

A method of detecting a biomarker by a machine learning-based detection system, the method comprising: identifying, by the detection system, a plurality of tiles corresponding to full slide image data of a tissue sample; generating, by the detection system, tile-level embedded data based on the plurality of tiles; generating, by the detection system, cellular level embedding data based on the plurality of tiles; and generating, by the detection system, a slide level prediction based on the tile level embedding data and the cellular level embedding data, the slide level prediction indicating the presence or absence of the biomarker in the tissue sample.

The utility model provides a ruminant living body muscle adipose tissue minimally invasive sampling device, which relates to the technical field of animal tissue sampling, and comprises an outer sleeve and an inner cutting rod, one end of the outer sleeve is provided with a needle, the other end of the outer sleeve is provided with a handle, and the outer sleeve is provided with a sampling groove close to the needle; the inner cutting rod is movably arranged in the outer sleeve, and a sampling knife is correspondingly arranged at the sampling groove. The animal sampling device has the advantages of being high in sampling efficiency, capable of reducing animal injuries, convenient, easy and fast to operate and low in cost.

A cartridge (2600) for use with a medical waste collection system (30). The cartridge includes a housing (2602, 2612) shaped to be removably attached to a receptacle (1699) integral with the housing. A sleeve (2640) is attached to the cartridge to be positioned in front of the housing. The sleeve is formed with a void space. A connector (2664) leading to the suction line for collection is attached to the cannula. A screen (2714) is removably mounted within the casing void space. When it is desired to capture tissue that may be contained in the fluid flow from the suction line, the screen is mounted within the cannula void space.

The present invention relates to an in vitro method for predicting the response to immunotherapy treatment of hepatocellular carcinoma in a subject, comprising the following steps: 1) A step of determining the energy metabolic state of a liver tissue sample from a subject, wherein the sample comprises pathological tissue and optionally non-pathological tissue, 2) A step of detecting the presence or absence of energy conversion resulting from a reduction in mitochondrial respiration in the pathological tissue by comparing it with a liver tissue sample of the subject or a non-pathological tissue from a standard non-pathological liver sample, Here: - If mitochondrial respiration is reduced in pathological tissue, the subject is less likely to respond to immunotherapy treatment, and - If mitochondrial respiration is not reduced in the pathological tissue, the subject is highly likely to respond to immunotherapy treatment.

The separation tube is mainly used for centrifugal crushing operation of tissue samples in the liquid nitrogen grinding process and specifically comprises a tube body and a tube cover installed at an opening in the upper end of the tube body, a grinding baffle ring and a grinding inner container which are distributed up and down are arranged in an inner cavity of the tube body in a sleeved mode, and the inner wall of an opening in the lower end of the grinding baffle ring is provided with an inclined face. The inclined face is opposite to the inner wall of the grinding inner container, and evenly-distributed grinding balls are installed on the inclined face and the inner wall of the grinding inner container. According to the invention, the grinding baffle ring and the grinding liner are respectively mounted at the upper end and the lower end of the inner cavity of the tube body, and the grinding balls are uniformly distributed on the inclined surface of the grinding baffle ring and the inner wall of the grinding liner, so that tissue samples in the tube body can collide with the peripheral grinding balls at a high speed when the tube body is subjected to rotary centrifugation, and crushing centrifugation is realized; a grinding medium does not need to be added, the link of screening the grinding medium is omitted, actual operation steps are optimized, operation is easy and convenient, and convenience is brought to actual centrifugal operation.

The present invention relates to sampling kits for the collection, storage and transport of a tissue sample of a test subject. The kit comprises a sampling tool having a tip section configured to collect the tissue sample. The collected tissue sample may be directly retained and stored in the tip section or may be transferred onto a tape element and stored thereon.

The application relates to the technical field of biological sample pretreatment, and discloses a high-efficiency separation method of a tissue cell extraction device. The method comprises the following steps: performing parameter calibration and reference loss modeling of a driving motor, and eliminating background interference of an actuator; applying a disturbance signal to a dissociation chamber, locking the characteristic resonance frequency of a tissue sample through frequency domain energy analysis, and performing pulsating crushing to accelerate the dissociation of a matrix structure; extracting the net torque of the motor in real time, deducing the fluid dynamic viscosity, adaptively adjusting the motor speed according to the viscosity drop trend, maintaining the fluid shear force in a preset target interval, and avoiding mechanical damage to cells; calculating the mechanical energy dissipation power in real time, and performing feedforward temperature control compensation to eliminate temperature overshoot caused by thermal conduction lag. The application realizes closed-loop control of the physical phase evolution of a heterogeneous tissue dissociation process, solves the limitation of a fixed parameter in a traditional method, shortens the dissociation time, and significantly improves the single-cell yield and survival rate.

An apparatus for storing tissue and other biological materials includes a separable flexible container that includes a first layer coupled to a second layer via a set of seals to define a first storage volume and a second storage volume. Separate tissue specimens are containable within the first storge volume and the second storage volume. A first portion of the first layer or the second layer defines a first opening into the first storage volume. A second portion of the first layer or the second layer defines a second opening into the second storage volume. The opening into the first volume and the opening into the second volume are positioned near opposite edges of the flexible container. The separable flexible container also includes a hinge to allow the first opening and the second opening to move from location on opposing edges of the separable flexible container to adjacent locations.

The invention provides a method for carrying out multi-term immunohistochemical staining by using a single tissue slice, which is characterized by comprising the following steps: S1, selecting and preparing a tissue slice to be detected, S2, analyzing pathological tissues and related information of an HE tissue slice, S3, marking, transferring, correcting and punching staining through holes of the HE tissue slice and the tissue slice to be detected, S4, preparing before immunohistochemical staining, and S5, carrying out multi-term immunohistochemical staining by using a single tissue slice. Removing the protective film on the bottom surface of the perforated transparent adhesive sticker, bonding the bottom surface of the transparent adhesive sticker with the wax mask tissues of the to-be-detected tissue slice, exposing the wax mask tissues of the to-be-detected tissue slice at the bottoms of the dyeing through holes corresponding to the transparent adhesive sticker respectively, and removing the protective film on the surface of the transparent adhesive sticker to obtain the to-be-detected tissue slice. And opening or closing the corresponding dyeing through holes by using the silica gel nail covers to form the to-be-detected tissue sample sheet with a plurality of dyeing holes. And more comprehensive detection results of a plurality of items can be obtained on only one tissue slice, so that the application range of immunohistochemical detection is expanded, the immunohistochemical detection time is shortened, the working efficiency is improved, and the detection cost is reduced.

An image analysis method for determining the biomedical state of a tissue sample. The method includes receiving a digital image of a tissue sample, identifying the number and location of A-type cells and B-type cells, obtaining an observed relative distribution, obtaining a reference relative distribution of expected distances between reference A-type cells and reference B-type cells, computing a proximity score as a difference of the reference relative distribution and the observed relative distribution, computing a combined score comprising the proximity score and the density of the A-type cells and / or the density of the B-type cells, and using the combined score for determining the biomedical state of the tissue sample and / or outputting the combined score.

Apparatuses and methods for recovering solid tissue and dislodged cells ("D-cells") from a biopsy are disclosed herein. In an embodiment, a biopsy container apparatus for recovering solid tissue and D-cells from a biopsy includes a receiving container and a sieve container. The receiving container has a receiving chamber with an upper opening and a watertight bottom surface. The sieve container is configured to be located at least partially within the receiving chamber and removed from the receiving chamber through the upper opening. The sieve container has a bottom sieve surface including a plurality of apertures configured to pass the D-cells from the biopsy but not the solid tissue from the biopsy into the receiving chamber after the solid tissue and the D-cells from the biopsy are placed into the sieve container.

Provided herein are compositions that include bone grinding and a tissue sample derived from intervertebral disc. The compositions may include or be used as therapeutics. For example, the compositions may be implanted into a subject's body, such as in a body part, organ, or tissue to cause a therapeutic effect in the subject. In some embodiments, the compositions may be used for cell therapy. In some embodiments, the compositions may be used to treat cancer. In some embodiments, cells, such as stem cells and / or bone marrow cells in the compositions of the present disclosure may be used to treat diseases such as cancer.

A biopsy system includes a biopsy device, a control module, and a tissue delivery tube. A biopsy device includes a probe, a needle, and a cutter. A needle extends distally from the probe. The cutter is movable relative to the needle to cut one or more tissue samples. A control module is in communication with the biopsy device and includes a sample tray configured to receive one or more tissue samples cut by the cutter. The tissue delivery tube is adapted to be connected between a tissue sample holder and a cutter of a biopsy device. The sample tray has a sampling configuration and an imaging configuration. The sample tray is configured to move between a sampling axis corresponding to the sampling configuration and an imaging axis corresponding to the imaging configuration to receive one or more tissue samples and subsequently image the one or more tissue samples received within the sample tray.

To provide a more automatable, consistent and comprehensive method for providing a diagnosis of the state of cells present in a patient sample.SOLUTION: The detection of pathological abnormalities in a tissue sample and / or a plurality of cells is a highly specialized and time-consuming task, usually performed by a group of selected clinicians and technicians. Described herein are methods for more automatable, consistent and comprehensive cell sample analysis to provide rapid and reliable detailed classification, for example, but not limited to cancer diagnosis, particularly diagnosis of the state of the cells present in the sample.SELECTED DRAWING: Figure 1A

The invention discloses a nucleic acid extraction-free direct RT-qPCR (reverse transcription-quantitative polymerase chain reaction) quantitative analysis method for RNA (Ribonucleic Acid) in tissue, which comprises the following steps: S1, preparing a tissue nucleic acid extraction-free lysis solution, mixing the tissue nucleic acid extraction-free lysis solution with a tissue sample, performing homogeneous crushing treatment, performing gradient centrifugation, and taking supernatant as a reverse transcription template; s2, designing a specific primer probe combination for RNA to be detected, performing reverse transcription on the reverse transcription template obtained in the S1, adding a reverse primer into a reverse transcription system during reverse transcription, and performing reverse transcription to obtain cDNA (complementary deoxyribonucleic acid); and S3, carrying out quantitative analysis on a tissue sample by taking the cDNA obtained in S2 as a template. According to the method provided by the invention, a nucleic acid extraction step is not needed, the detection time is remarkably shortened, the cost is reduced, meanwhile, target RNA is prevented from being lost, the accuracy and the stability of a quantitative result are improved, and the method is suitable for efficient detection of RNA in various tissues.

The invention relates to the technical field of virus detection, in particular to a fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection probe 1106VP and / or 1106WP and primers 1106VWF and 1106VWR. The invention also discloses a fluorescent RT-PCR detection reagent, a fluorescent RT-PCR detection kit, a fluorescent RT-PCR detection probe, a primer, the fluorescent RT-PCR detection reagent and the application of the fluorescent RT-PCR detection kit in identification of a porcine reproductive and respiratory syndrome NJ-1106R strain and a porcine reproductive and respiratory syndrome non-NJ-1106R strain. The probe and the primer disclosed by the invention are high in specificity and high in sensitivity; the accuracy is high, and the accuracy rate of blind sample detection is 100%; the kit is high in universality, can be used for detecting virus liquid for cell culture in a laboratory, and can also be used for detecting porcine serum and tissue samples in clinical samples. The method is an effective method for identifying the PRRSV NJ-1106R vaccine strain and other PRRSV wild strains.