60 results about "Tissue sampling" patented technology

The invention relates to the technical field of medical equipment, in particular to a tumor puncture sampling device which comprises a body, a cannula, a needle core, a limiting mechanism, an excitation mechanism and a plugging mechanism. By changing the shape of the front end of the needle core, the end face of the front end of the sampling groove is flush with the end face of the front end of the needle core, so that the tumor tissue is directly cut in the process that the needle core pierces the tumor tissue, and the problem that in the prior art, a puncture needle with the front end being a solid cylinder pushes away the tumor tissue is solved; when the cannula is pierced, a tissue sample and tumor tissue around the tissue sample are cut off, and when the cannula moves by a preset distance, the plugging mechanism cuts off the front end of the tissue in the sampling groove, so that the sample is completely separated from the tumor tissue, and the plugging assembly plugs a gap between the needle core and the cannula, so that the tissue sample is prevented from being separated from the sampling groove. The problem that in the prior art, when a puncture sampling needle conducts puncture sampling, the needle core pushes away tumor tissue, and consequently the tissue sampling amount is insufficient is solved.

The utility model discloses a high-fidelity quick sampling device of a high-grade biological safety dissecting room, which relates to the technical field of biological sampling devices, and comprises a barrel, a first end of the barrel is provided with a cavity, an inlet communicated with the cavity is arranged at the first end of the barrel, scale marks are arranged outside the barrel, a first cutting part is arranged at the first end of the barrel, and a second cutting part is arranged at the second end of the barrel. The first cutting part has a cutting state and an idle state; the operation part is connected with the cylinder body, the operation part and the inlet are arranged in an avoiding manner, and a barrier layer is arranged between the operation part and the cylinder body; the blocking layer enables substances such as pathogens not to be attached to the operation part, the operation part is detachably connected with the second end of the cylinder body and the blocking layer, and therefore the situation that the pathogens attached to the cylinder body and the blocking layer are attached to the next biological tissue, errors are caused to follow-up identification and analysis of the next biological tissue can be avoided as far as possible. And high fidelity during sampling of different biological tissues is realized.

The invention discloses stomach wall tissue biopsy forceps under a laparoscope, and belongs to the technical field of medical instruments. The biopsy forceps aim at solving the problems that existing biopsy forceps are low in sampling efficiency and inconvenient in angle adjustment. The device comprises a fixed pipe and a rotating pipe, a first pull rod is slidably connected into the fixed pipe, a second pull rod is slidably connected into the rotating pipe, an extension column is integrally formed at one end of the second pull rod, a limiting ball is integrally formed at one end of the extension column, and a limiting groove matched with the extension column and the limiting ball is formed in one end of the first pull rod; a connecting part is arranged between the fixed pipe and the rotating pipe and is used for connecting the fixed pipe and the rotating pipe; biopsy forceps are installed at the end, away from the fixed tube, of the rotating tube, and the biopsy forceps and the rotating tube rotate through a connecting part. The driving part is arranged at one end of the fixing tube and used for driving the first pull rod to move, a plurality of samples can be taken through one-time insertion, angle adjustment is flexible, operation is labor-saving, and the device is used for stomach wall tissue sampling under a laparoscope.

The utility model provides a ruminant living body muscle adipose tissue minimally invasive sampling device, which relates to the technical field of animal tissue sampling, and comprises an outer sleeve and an inner cutting rod, one end of the outer sleeve is provided with a needle, the other end of the outer sleeve is provided with a handle, and the outer sleeve is provided with a sampling groove close to the needle; the inner cutting rod is movably arranged in the outer sleeve, and a sampling knife is correspondingly arranged at the sampling groove. The animal sampling device has the advantages of being high in sampling efficiency, capable of reducing animal injuries, convenient, easy and fast to operate and low in cost.

The invention relates to the technical field of neutrophile granulocyte separation, in particular to a mouse neutrophile granulocyte separation method which comprises the following steps: (1) anesthetizing and disinfecting a mouse; (2) tissue sampling; (3) tissue digestion; (4) preparing a cell suspension; (5) purifying cells; (6) cell culture; according to the method disclosed by the invention, isoflurane is adopted in the anesthesia disinfection step, so that central nervous activity of mice can be rapidly inhibited, tissue damage caused by stress reaction is avoided, and chemical stimulation can be reduced while body surface microorganisms are cleared through combination of ethanol disinfection and sterile normal saline flushing, and aortic tissues with intact activity are provided for subsequent material taking. In the compound enzyme digestive juice, collagenase I can specifically degrade aortic wall collagenous fibers, trypsin dissociates adhesion connection between cells, neutral protease assists in decomposing elastic fibers, and DNA enzyme I is supplemented to inhibit cell aggregation, so that the contradiction between incomplete digestion and large cell damage of the traditional enzyme juice is effectively solved.

The application discloses a peanut-shaped magnetorheological capsule robot living tissue sampling controller based on IGJO, which comprises mechanical analysis of a living tissue sampling module for the peanut-shaped magnetorheological capsule robot, research on the driving effect of an external driving permanent magnet on the capsule robot, establishment of the living tissue sampling controller of the capsule robot, design of an improved golden jackal optimization algorithm, introduction of Fuch mapping theory and an elite population strategy in the population initialization stage, optimization initialization of the golden jackal population, introduction of an adaptive inertia weight omega and a searcher position updating strategy in the improved sparrow algorithm in the iterative output stage of the population, repeated optimization of the output solution of the golden jackal population, and effective improvement of the global search capability of the algorithm. The application improves the algorithm performance of the golden jackal algorithm, avoids falling into local optimization, realizes the optimization and adjustment of the living tissue sampling controller parameters of the capsule robot, optimizes the control effect, and improves the control precision.

The utility model discloses a centrum focus tissue sampling device which comprises an outer sleeve and an inner sleeve and further comprises an inner core sampling structure, the inner sleeve is clamped in the outer sleeve and can be disassembled and assembled, the inner core sampling structure is movably arranged in the inner sleeve, and the inner core sampling structure is connected with the outer sleeve. The inner core sampling structure is matched with the inner sleeve to rotate to sample focus tissues; the inner core sampling structure comprises an inner core rod, a sampling rod is arranged on one side of the inner core rod, a cone part is arranged at the end of the sampling rod, and a threaded tip is arranged on the sampling rod. By adopting the closed structure of the inner core sampling structure and the inner sleeve, the sampling of the focus tissue is facilitated, the focus tissue at the same part can be sampled for multiple times, the operation is convenient, and the replacement and sleeve connection are not needed.

The invention aims to provide a general sampling device for pathological diagnosis, and relates to the technical field of medical instruments, tissues and stationary liquid can be sucked into a filter cartridge by pulling a pressurizing rod backwards, and then a filter is mounted on an outlet pipe; liquid in the filter cartridge can be pushed to the filter by pushing the pressurizing rod forwards, and fine flocculent tissues in the stationary liquid are filtered on a filter membrane of the filter and are extruded to be left in the outlet pipe, the extension pipe and the front end of the filter cartridge; when in use, the filter is detached, the push head of the push rod is slid to extend out of the push groove to push tissues in the front end of the filter cartridge out of the outlet pipe and the extension pipe, so that cylindrical tissue blocks are obtained, and the tissues can be completely collected, taken and subsequently embedded and sliced conveniently; the push rod slides to control the scraper to slide out of the piston head to be in contact with the front end of the filter cartridge to scrape the tissues remaining in the filter cartridge into the outlet pipe, so that the tissues are conveniently pushed out by the push rod and are prevented from being adhered to the front end of the filter cartridge.

The present application relates to the technical field of medical equipment, in particular to a puncture sampling device for tumor, which comprises a main body, a sleeve, a needle core, a limiting mechanism, an exciting mechanism and a plugging mechanism; the present application changes the shape of the front end of the needle core, so that the end face of the front end of the sampling groove is flush with the end face of the front end of the needle core, thereby directly cutting the tumor tissue in the process of the needle core piercing into the tumor tissue, and the problem that the tumor tissue is pushed away by the puncture needle with a solid cylindrical front end in the prior art does not exist; the tissue sample and the tumor tissue around it are cut off in the process of the sleeve piercing in; when the sleeve moves a preset distance, the plugging mechanism cuts off the front end of the tissue in the sampling groove, so that the sample is completely separated from the tumor tissue, and the plugging component plugs the gap between the needle core and the sleeve to prevent the tissue sample from separating from the sampling groove. The present application solves the problem that the tissue sampling amount is insufficient due to the tumor tissue being pushed away by the needle core when the puncture sampling needle in the prior art performs puncture sampling.

The invention relates to a multifunctional capsule robot integrating dual-channel biopsy, drug administration and anchoring functions, which comprises a shell, a capsule, a capsule, a capsule and an anchoring device, wherein a bracket is arranged in the shell; the three-degree-of-freedom magnetic driving assembly is arranged on the bracket, and the three-degree-of-freedom magnetic driving assembly is arranged on the bracket; the tool switching mechanism is used for switching different tool cabins, and the tool switching mechanism is installed on the support; the four tool cabins respectively have the functions of 30G biopsy, 25G biopsy, drug A administration and drug B administration, are used for supporting the realization of double-channel biopsy and double-channel administration, and are mounted on a four-phase tool wheel disc of the tool switching mechanism; and the deformation anchoring mechanism is used for being fixed near a focus, and the deformation anchoring mechanism is connected with the support. The device is used for diagnosis and treatment of human body lumen organs such as digestive tracts, is suitable for multi-part tissue sampling, multi-drug precise administration and stable anchoring operation under the non-invasive or minimally invasive condition, realizes function cooperation through the four-phase tool wheel disc, and flexibly switches the working state between two-channel biopsy and drug administration; and an integrated solution is provided for diagnosis and treatment of intracavity diseases.

The invention relates to a bronchoscope matching sampling assembly and an automatic forceps examination system, and relates to the technical field of medical auxiliary instruments, the assembly comprises a forceps examination auxiliary device used for clamping biopsy forceps and driving the biopsy forceps to move to execute tissue sampling action of bronchoscope forceps examination; the front end guide plate is arranged at the foremost end of the clamp inspection auxiliary equipment body or the bearing table and is provided with a first positioning guide hole allowing biopsy forceps to penetrate through, the front side of the front end guide plate is integrated with a closed spray washing cavity, a cavity plate of the spray washing cavity is provided with a second positioning guide hole allowing the biopsy forceps to penetrate through, and the first positioning guide hole and the second positioning guide hole are aligned; a spray-washing head is arranged in the spray-washing cavity, and the liquid outlet direction faces a spray-washing area between the first positioning guide hole and the second positioning guide hole; a liquid collecting container is communicated with the lower part of the spray-washing cavity; the forceps head position detection assembly triggers a control signal when detecting that the forceps head position of the biopsy forceps reaches a spray washing area, stops the traction action of the forceps detection auxiliary equipment on the biopsy forceps, and starts the spray washing action.

The utility model relates to the technical field of tissue sampling, in particular to a fur opening device for animal subcutaneous tissue sampling. The distraction device comprises a first distraction rod, and a second distraction rod is rotationally arranged in the middle of the surface of the first distraction rod; threaded holes are formed in the surfaces of the first distraction rod and the second distraction rod, positioning mechanisms are arranged in the threaded holes in a threaded mode, and connecting rods are fixedly arranged at the ends, away from the positioning mechanisms, of the first distraction rod and the second distraction rod. According to the fur opening device for animal subcutaneous tissue sampling, by arranging the opening mechanisms, a first opening rod and a second opening rod are separated to open animal fur, at the moment, the two sets of opening mechanisms are arranged on the inner side of the animal fur, a fixing frame is arranged on the outer side of the animal fur, and an inner buckling plate is matched with the fixing frame to open the animal fur; the effect of opening animal fur is achieved, personnel can sample subcutaneous tissue conveniently, and the situation that animal fur is attached to the subcutaneous tissue and influences sampling is reduced.

This invention discloses a clinically practical AI-assisted diagnostic system based on the tissue sampling site of a medical case. The system includes: an acquisition module for acquiring case diagnostic information; a first determination module for determining label information for each tissue sampling site in each case based on the case diagnostic information; a second determination module for determining the target slide corresponding to the target tissue sampling site based on the label information; a preprocessing module for performing image preprocessing on the target slide to obtain a target slide in feature vector form; and a fusion inference module for performing image fusion inference on the target slide converted to feature vector form to obtain the AI-assisted diagnostic result. This system can accurately and efficiently simulate the diagnostic process of a pathologist, improving the capabilities of AI-assisted diagnosis.

The invention relates to the technical field of cell tissue sampling, and discloses a cancer tissue slice sampling device for tumor immunoassay, the cancer tissue slice sampling device comprises a case, a wax coating constraint assembly, a slice shifting assembly, a slice linear driver, a slice cutter, a feeding linear motor and a lifting bracket, and the wax coating constraint assembly is located in the case. The invention particularly provides a cancer tissue slice sampling device for tumor immunoassay, which can stably position a wax coated sample and can take slices one by one after integral cutting.

The utility model discloses an integrated device for transferring and preparing small sample tissues after sampling, which belongs to the technical field of storage boxes and comprises a storage shell, a storage box used for storing the small sample tissues and a refrigerating system used for refrigerating the storage box and arranged in the storage box. The storage box is arranged on the refrigeration equipment, a storage cover is arranged on the storage box, and a storage groove is formed in the storage box; according to the device, after required operation samples are taken, the required operation samples can be placed in the storage grooves in the storage box to be stored, and the required samples are registered and marked and then placed in the corresponding label bags to be stored, so that the moisture-preserving low-temperature transportation of the samples and the accuracy of sample information are realized; and liquid nitrogen can be injected from the connecting pipeline preferentially to fix the sample, so that a powerful foundation is laid for taking out the OCT embedded frozen section from the sample subsequently.

The application provides an animal tissue sampling device and belongs to the technical field of cell culture. The technical scheme is as follows: a base and a sampling mechanism arranged on the base, the base is provided with an operation sleeve through a pair of inverted L-shaped supporting rods, the sampling mechanism is movably arranged in the operation sleeve, the sampling mechanism comprises a fixing frame movably arranged in the operation sleeve, the fixing frame is provided with an outer sampling sleeve, the fixing frame comprises a U-shaped handle, the U-shaped handle is respectively provided with a first fixing cylinder and a second fixing cylinder at both ends, and the outer sampling sleeve is arranged between the first fixing cylinder and the second fixing cylinder. The application has the advantages of simple structure, convenient use, fast sampling, great guarantee of sample pollution, and improved efficiency and quality of scientific research.

A catheter-based biopsy system integrates real-time optical coherence tomography (OCT) imaging with a multi-degree-of-freedom biopsy needle for precise tissue sampling within luminal organs. The system comprises a segmented handle, an outer sheath, a steerable needle, and an imaging stylet housed within the needle. The imaging stylet includes a metasurface-based optical lens for acquiring cross-sectional images through an imaging window in the needle. Independent handle segments control the extension of the needle and the positioning of the imaging stylet. The system supports multiple operational modes, including imaging, image-guided biopsy, and tissue retrieval, with optional encoder-based tracking for enhanced navigation. The integrated design enables targeted fine needle aspiration and biopsy under direct visualization, improving diagnostic accuracy and procedural efficiency.

The utility model relates to the field of agricultural biotechnology discloses a kind of root tip material taking positioner for seed source purification, including machine body, the inside right end of machine body is fixedly connected with fixed mechanism, the fixed mechanism is used to be fixed to different size culture dish, the inside top of machine body is equipped with moving mechanism, the moving mechanism is used to take material to root source;The fixed mechanism includes fixed half ring, the fixed half ring is fixedly connected in the inside right end of machine body, the right side of fixed half ring is equipped with moving half ring, the bottom of machine body is equipped with sliding slot. In the utility model, support frame is fixed in machine body, and it supports double rack movement, drives moving half ring to slide in the sliding slot of the bottom of machine body, fixed half ring is fixed in machine body, and the cooperation of both realizes the fixation of different size culture dish, and effectively fixes the culture dish containing Chinese medicinal material root source.

The application relates to the field of thyroid tissue sampling technology and discloses a thyroid tissue sampling needle with a negative pressure adsorption function, which comprises a guide stab needle and a hollow needle tube, a mounting block is arranged on the hollow needle tube, a communication hole in communication with the hollow needle tube is arranged on the mounting block, a communication pipe is arranged on the side wall of the mounting block, the communication pipe is in communication with the communication hole and an external negative pressure device, a fixing block is arranged on the top of the mounting block, a fixed magnetic ring is arranged in the fixing block, a fixed magnetic block and a positioning magnetic block are embeddedly arranged on the guide stab needle; when the fixed magnetic block is attracted to the fixed magnetic ring, the guide stab needle is arranged in a posture that the needle tip part penetrates through the needle tip part of the hollow needle tube; when the positioning magnetic block is attracted to the fixed magnetic ring, the guide stab needle is arranged in a posture that the needle tip part moves into the communication hole, so that the communication pipe is in communication with the hollow needle tube through the communication hole; the application has the advantages of coherent operation, guaranteed sample quality, avoided sample pollution and loss.

A medical system including a flexible tissue sampling mechanism having a cannula and a stylet disposed within the cannula. The medical system includes at least one sensor configured to generate sensor data indicating a shape of the flexible tissue sampling mechanism. Additionally, the medical system includes a drive mechanism configured to generate a drive force that varies based on the sensor data to advance the cannula relative to the stylet to take a tissue sample.

The invention relates to the technical field of medical instruments, and discloses colposcope biopsy forceps capable of synchronously sampling at multiple points, the inner wall of a forceps tube is fixedly connected with three adjustable biopsy forceps components, the inner wall of a handle sleeve is fixedly connected with a synchronous opening and closing driving component, and three movable forceps clips are driven by a telescopic bracket to respectively approach to corresponding fixed forceps clips, so that the synchronous opening and closing of the forceps is realized. Synchronous closing of jaws of the three groups of movable forceps holders and the fixed forceps holder is realized, so that a plurality of tissue samples can be obtained from different parts at the same time in single operation, the biopsy sampling efficiency is improved, and the pain and bleeding risk of a patient are prevented from being increased; the fixed forceps holder and the movable forceps holder are independently moved and adjusted to a preset depth, so that more accurate tissue sampling depth adjustment is realized, and the sampling positioning accuracy and the subsequent sample detection accuracy are improved; independent and rapid collection of a plurality of samples is realized, cross contamination of the plurality of samples is avoided, and the reliability of subsequent pathological results is ensured.

The invention belongs to the technical field of medicines, and particularly relates to an experimental method for treating liver cancer by using oleanolic acid. Comprising an in-vitro experiment method and an in-vivo experiment method. The in-vitro experiment method comprises the following steps: S1, cell culture; s2, cell proliferation and migration; s3, detecting cell apoptosis and a cell cycle; s4, performing immunoblotting; s5, carrying out real-time quantitative PCR; the in-vivo experiment method comprises the following steps: step 1, performing chick embryo chorioallantoic model experiment; and 2, carrying out tumor-bearing experiment, treatment and tissue sampling on the mouse. The application of the oleanolic acid in liver cancer treatment is systematically researched for the first time, it is found that the oleanolic acid has the remarkable effects of inhibiting proliferation, inducing apoptosis, resisting angiogenesis and the like on liver tumors through the synergistic effect of multiple mechanisms, the remarkable curative effect on cancer mice is achieved, and a new medicine choice is provided for liver cancer treatment.

Disclosed embodiments include devices, systems, and methods for securing a control device of an elongate instrument to an insertion device. In an exemplary embodiment, the device includes a fitting configured to couple the control device to a port of the insertion device used to deliver the elongate instrument to a target location. A bushing extends from the fitting and is configured to be inserted into an opening of the port of the insertion device. The elongate instrument is movably extendable through the bushing. A first seal member is disposed on an outer surface of the bushing to seal the outer surface of the bushing against an inner surface of the opening of the port. A second seal member is disposed on the outer surface of the elongate instrument to movably seal the outer surface of the elongate instrument.

The utility model provides a tissue sampling tool in an endoscopic surgery, which relates to the technical field of medical instruments and comprises an electronic controller, a rotating shaft disc is arranged on the outer side of the electronic controller, a sampling and wire drawing motor is mounted on the lower side of the electronic controller, a wire drawing button is mounted on the outer side of the sampling and wire drawing motor, and the rotating shaft disc is arranged on the rotating shaft disc. The extension hose made of polytetrafluoroethylene has an extremely low friction coefficient, so that when the catheter passes through an endoscope working channel or moves in a human body, the resistance borne by the catheter is very small, the biocompatibility is good, immune response or inflammatory response of the human body cannot be caused, and the optical fiber lamp can be connected with the clamping plate through the characteristic that the optical fiber lamp can be connected with the clamping plate. The optical fiber lamp is arranged on the sampling forceps head and directly illuminates the sampling forceps head, medical staff can conveniently conduct more fit operation, the micro camera can move along with movement of the sampling forceps head so that control can be better achieved, and due to the fact that the micro camera and the optical fiber lamp are symmetrically arranged, help can be better provided when the sampling forceps head conducts forceps.

The invention relates to the technical field of animal brain tissue sampling, in particular to a low-temperature perfusion device which is characterized in that a recycling groove is formed in the edge of an operation plate, the bottom face of the recycling groove is hollowed out, a binding plate and two stacked low-temperature perfusion assemblies are arranged above the operation plate, and the recycling assembly is arranged in an operation frame; and an operation cooling plate is mounted below the binding plate. The using method of the low-temperature perfusion device comprises the steps that after the limb binding pieces are adjusted, an experimental animal is bound to the upper portion of the binding plate, the two low-temperature perfusion assemblies are filled with a PBS solution and a PFA solution, and then the two low-temperature perfusion assemblies are controlled to conduct PBS solution and PFA solution perfusion on the experimental animal. According to the low-temperature perfusion device, the perfusion function, the low-temperature function and the toxic waste liquid recovery function are integrated, the overall use efficiency of the low-temperature perfusion device is higher, the safety of operators and the environment are better protected, and more importantly, perfusion can be accurately conducted at the small liquid perfusion speed through the ingenious design of the low-temperature perfusion assembly.

The invention discloses a puncture guide device for an abdominal tumor, relates to the field of medical surgical instruments, and solves the problems that when an existing puncture guide device for the abdominal tumor is used, it is difficult to seal a puncture channel in a multi-section mode in the using process, and diffusion of internal tissue and fluid is avoided. Comprising a guiding sleeve, a fixing block, a sampling sleeve, a puncturing mechanism and a sealing mechanism, the puncturing mechanism comprises a guiding inner core and a sampling inner core, the sealing mechanism comprises an expansion sponge and a non-return piece, and an annular groove is formed in the guiding sleeve. Tissue sampling operation is achieved through cooperation of the sampling sleeve and the sampling inner core, the situation that fluid around the guiding inner core flows back into the guiding sleeve is reduced through the non-return piece, the output state of the multiple sets of expansion sponges is controlled through the sealing mechanism, multi-section sealing operation on the needed position is achieved, and the probability that a tumor is diffused through a puncture channel is reduced.

The present application relates to the technical field of biological information, and particularly relates to a non-small cell lung cancer medical data mining method.The data mining method detects blood samples of patients before medication, avoids invasive tissue sampling of patients, and compared with the limitation of a traditional method which only relies on a small number of indexes (such as gene expression, mutation, etc.), complex data at a single cell level are used to ensure the robustness of a deep learning model.

Systems, devices, and methods for planning an ultrasound-guided tissue sampling procedure are disclosed. An exemplary system comprises an ultrasound imaging device for generating ultrasound images of an anatomical target in a real-time ultrasound field of view (FOV), a display for showing the ultrasound images, and a control unit. Before a tissue sampling device is extended into the ultrasound field of view, the control unit displays graphical user interface elements (UIEs) that show a predicted nominal trajectory of the sampling device intersecting the anatomical target object in the ultrasound field of view. As the tissue sampling device is extended into the ultrasound field of view, the control unit determines one or more real-time trajectory parameters and updates the UIEs.The control unit displays the updated UIEs to provide visual feedback on whether the tissue sampling device will intersect the anatomical target.

The utility model discloses a disposable intraoperative frozen pathological tissue sampling and collecting device, which belongs to the technical field of pathology inspection, and comprises a collecting box, the front end of the collecting box is provided with a sticking groove I, and the rear end of the collecting box is fixedly connected with two rotating seats; through cooperation of the collecting box and the sealing cover mechanism, when frozen pathological tissue in an operation needs to be taken for inspection, the pathological tissue can be effectively prevented from diffusing an infection source to the outside, meanwhile, the anti-falling function is achieved, application forms needed during inspection can be stored through the storage assembly, and the storage assembly is convenient to use. The application form can be effectively prevented from being lost, the device can be provided with a sealing strip through the pasting assembly, the sealing strip can be conveniently and rapidly torn down and pasted to the outer surface of the device, the function of sealing a pathological sample can be achieved, and the practicability of the device is improved.

The invention belongs to the field of biotechnology research, and describes a method for monitoring tomato fusarium wilt and early prevention and treatment of diseases based on minimally invasive tissue sampling. The method comprises the following steps: puncturing a tomato stem base through a sterile syringe needle to obtain trace vascular bundle tissues, and performing rapid nucleic acid extraction; then, whether the plant is infected or not is judged 5-7 days before typical symptoms appear through an isothermal amplification nucleic acid detection technology, the positive detection rate of a loop-mediated nucleic acid isothermal amplification (LAMP) detection method is 47.6%, and the positive detection rate of a recombinase-mediated nucleic acid isothermal amplification (RAA) detection method is 89.71%; finally, for the detected positive plants, in the window period when disease symptoms do not appear, trifluoropyridylamine and bacillus velezensis JSBV63 are adopted for root irrigation treatment in time, the progress of plant diseases can be controlled and blocked, and the control effect can reach 90% or above after two times of pesticide application. The combination of minimally invasive sampling, isothermal nucleic acid amplification detection and medicament treatment realizes a whole set of complete fusarium wilt prevention and treatment technical process from early diagnosis of pathogen infection to disease treatment.