61 results about "Skin sample" patented technology

The invention discloses a skin LC-OCT image segmentation method based on improved GAN and multi-feature fusion. The skin LC-OCT image segmentation method comprises the following steps: collecting a skin B-scan sample by adopting LC-OCT equipment and storing the skin B-scan sample as an image; the method comprises the following steps: constructing a generator fusing reflection filling, multi-scale coding-decoding, self-attention and residual blocks, cooperating with a multi-scale fusion discriminator and a weight sharing twin network, and realizing high-fidelity denoising and enhancement through joint optimization of perception loss, multi-scale SSIM loss and Wasserstein adversarial loss; preprocessing and positioning a skin area based on a threshold value or morphology and normalizing the skin area; gradient is extracted from the de-noised image, and dynamic path search is carried out in combination with a gradient difference minimum criterion and a random forest; and after adaptive sampling and spline fitting are carried out on the path, candidates are generated through a random forest, and fracture or dislocation of a focus area is corrected. Therefore, accurate segmentation of the LC-OCT image of the skin layer can be realized in a high-noise environment, a high-quality enhanced data set can be constructed, and a reliable basis is provided for early diagnosis of skin diseases.

Provided are a method for preparing a protein marker for predicting the risk of developing atopic dermatitis or the risk of allergen sensitization in infants, and a method for predicting the risk of developing atopic dermatitis or the risk of allergen sensitization using said protein marker.　More specifically, the method for preparing a protein marker for predicting the risk of developing atopic dermatitis includes recovering at least one kind of protein selected from FGG, FGA, FGB, FN1, IGHG1, IGHG2, IGHG3, IGHG4, C3, C4B, C7, CFB, CFH, EPX, CLC, RNASE3, RNASE2 and PRG2 from a sample collected from the skin of an infant subject.

Improved allografts having controlled and consistent properties and useful for soft tissue repair, including breast reconstruction, and other surgical procedures, are disclosed. In one embodiment, an improved acellular dermal matrix (ADM) is produced from a skin sample, where the ADM consists essentially of a single dermal tissue type (e.g., papillary or reticular) and comprises a collagen matrix having substantially uniform density and porosity. In another embodiment, two ADMs are produced from the same dermal tissue type (e.g., papillary or reticular) of the same skin sample, each of which consists essentially of reticular dermis, and comprises a collagen matrix having substantially uniform density and porosity. All such improved ADMs are suitable for use in breast reconstruction and other tissue repair and modification surgery procedures.

The invention relates to the technical field of frozen slicing, in particular to a mammal skin embedding method for a spatiomics technology, which comprises the following steps: acquiring an in-vitro skin sample which comprises an epidermal layer, a corium layer and optional subcutaneous tissue; shearing the fat in the skin sample under the condition a to remove the fat in the skin sample, and freezing and embedding the fat-removed skin sample. The mammal skin embedding method provided by the embodiment of the invention provides a standardized overall scheme for sample analysis of mammal skin and hair follicle tissues, is beneficial to obtaining tissue slices with high quality, complete morphology and good cell state, and realizes space omics sequencing with high sample utilization rate.

The invention relates to a high-speed atomic force microscope (AFM, 1) for measuring a relevant sample (S), such as a skin sample, the AFM having a sensor unit (SU, 10) comprising a light source (11) and a collimating lens (12), the collimating lens being mechanically displaced by a collimating lens actuator (12 '). An AFM cantilever having an AFM tip (14) is arranged relative to the photodetection beam (15) for providing a focus error signal (FES) indicative of mechanical engagement with the sample. The collimating lens (12) is arranged to be adjusted by a collimating lens actuator (12 ') along the beam path to compensate for at least thermal drift in the focus error signal (FES). According to the invention, the improved high-speed AFM can be obtained through the internal thermal drift compensation mechanism. The high-speed AFM of the embodiment of the invention can have a theoretical linear scanning speed which can reach 700000 um / s, which allows one-time imaging to be completed within about 3.4 seconds.

The utility model relates to a fungus sampling device which comprises a handle and a collecting bottle, a mounting groove is formed in the bottom of the handle, a mounting block is arranged in the mounting groove, a scraper is mounted on the outer surface of the mounting block, an inserting hole is formed in the side surface of the mounting block, and a mounting mechanism capable of being matched with the inserting hole in an inserting mode is arranged on the outer surface of the handle. A groove is formed in the side face, away from the installation mechanism, of the handle, a negative pressure pipe is arranged in the groove, a fan-shaped suction nozzle is installed at one end of the negative pressure pipe and arranged on the outer side of the edge of the scraper, and a connecting end is installed at the other end of the negative pressure pipe and matched with the upper end of the collecting bottle. Skin sample scraps scraped by the scraper can be sucked into the collecting bottle to be collected, the sampling efficiency is improved, the situation that the samples fall to infect others is avoided, through the arrangement of the lifting mechanism, after the samples are taken, the exhaust fan can be driven to move downwards, and then the collecting bottle can be conveniently taken down and taken to a clinical laboratory to be examined.

This application discloses a method and apparatus for constructing and applying a Parkinson's disease tendency prediction model, relating to the field of Parkinson's disease tendency prediction technology. The method includes: collecting skin samples from different sexes, different skin sampling sites, normal individuals, and Parkinson's patients; extracting DNA and detecting biomarkers from the skin samples to obtain skin biomarker data; the skin biomarker data includes the abundance of fungal biomarkers and bacterial biomarkers; constructing a training sample set from the skin biomarker data from different sexes, different skin sampling sites, normal individuals, and Parkinson's patients, and training a machine learning model to derive a Parkinson's disease tendency prediction model. This application proposes a non-invasive combination of core skin microecological biomarkers that takes into account "bacterial and fungal dual communities, sex specificity, and sampling site differences," enabling non-invasive and accurate prediction of Parkinson's disease tendency.

The invention provides a method for preparing a skin graft composition comprising (a) obtaining, from a subject requiring a skin layer patch or dressing, a skin sample, (b) placing the skin sample in a container with a sterile medium, (c) separating fibroblasts and keratinocytes from the skin sample to obtain a solution comprising fibroblasts and keratinocytes or reshaping and resizing the skin sample to obtain a solution comprising the reshaped and resized skin sample, and (d) combining the fibroblasts and the keratinocytes with a matrix to thereby form a skin graft composition. The invention also provides a method for providing a skin graft composition to a subject, as well as a method of providing a skin layer patch or dressing, or a skin graft, to a subject in need thereof.

The invention discloses a multi-stage cooling process for keeping the completeness of an alloy oxide skin, which adopts four-stage temperature control cooling and specifically comprises the following steps: in the first stage, cooling a sample from a heating temperature to 850-900 DEG C at a rate of 8-15 DEG C / min; in the second stage, heat preservation is conducted for 5-10 minutes in the temperature interval of 850-900 DEG C; in the third stage, the temperature is reduced to 240-260 DEG C from 850-900 DEG C at the speed of 3-5 DEG C / min; and a fourth stage: cooling from 240-260 DEG C to room temperature at a rate of 5-10 DEG C / min. By adopting the process disclosed by the invention, a complete oxide skin sample can be provided for researchers, and the process is used for accurately measuring the thickness of an oxide layer, analyzing component distribution and researching the characteristics of an interface transition layer, so that an alloy oxidation mechanism is deeply understood, and a theoretical basis and data support are provided for optimizing a production process and reducing oxide skin generation.

The present application relates to the field of aircraft surface laser advanced manufacturing technology, and discloses a laser removal method of aircraft surface stealth coating capable of identifying substrate damage response, comprising the following steps: 1) fixing a thermocouple on the bottom surface of the aircraft skin sample to be removed, and connecting the thermocouple with a temperature collector and a control end to record the temperature change of the bottom surface of the sample in real time; 2) placing the aircraft skin sample on a workbench with the stealth coating facing the laser; 3) turning on the laser, adjusting parameters, and removing the stealth coating; 4) observing the temperature change of the bottom surface of the sample in real time at the control end, and stopping the laser removal immediately when the temperature curve shows a steep increase trend. The present application links the substrate damage and the visual temperature change by using the temperature response caused by the laser directly acting on the substrate, converts the unobservable removal effect into the temperature curve change that can be monitored in real time, solves the pain point that the substrate damage cannot be identified in the laser removal process, and is convenient to operate and low in price.

A system and method for testing an effect of light exposure on a skin sample include a solar simulator arranged to administer a combination of visible light (VL) and long wavelength ultraviolet radiation (UVA1) to the skin sample. The solar simulator includes a lamp for generating a light beam and at least one customized filter for receiving the light beam and emitting a VL+UVA1 spectral output having a wavelength range of 370-700 nm for irradiating the skin sample.

The present invention relates to a system for collecting a skin sample from a test subject, the system comprising a collector device comprising an adhesive surface, said adhesive surface configured to collect the skin sample via adhesion upon contact of the adhesive surface with the skin sample. The present invention also relates to a method for collecting a skin sample from a test subject, the method comprising collecting the skin sample with a system comprising a collector device, said collector device comprising an adhesive surface, said adhesive surface configured to collect the skin sample from the test subject via adhesion upon contact of the adhesive surface with the skin sample.

The invention relates to the field of tissue engineering biological materials, in particular to an epidermal stem cell-loaded imitation skin ridge microneedle and a preparation method and application thereof, and the method comprises the following steps: providing a target skin sample; a three-dimensional structure of the target skin sample is obtained through laser femtosecond three-dimensional reconstruction, image processing software is adopted for analysis and quantification, and skin ridge size parameters of the target skin sample are obtained; preparing a microneedle mold according to the skin ridge size parameters; respectively preparing a needle tip solution and a substrate solution, and adding the epidermal stem cells in the culture logarithmic phase into the needle tip solution to obtain a mixed solution; filling a microneedle area of a microneedle mold with the mixed solution, and performing centrifugal treatment; and filling a substrate area of a microneedle mold with the substrate solution, carrying out illumination crosslinking, and demolding to obtain the epidermal stem cell-loaded artificial skin ridge microneedle. The method can realize stable survival of epidermal stem cells in the artificial skin ridge microneedle, and has the effects of reconstructing a skin wound skin ridge structure and improving wound healing quality.

The invention relates to the technical field of biology, in particular to a primer group and a method for detecting malassezia furfur. The primer group for detecting malassezia furfur, disclosed by the invention, comprises the nucleotide sequences of primers as shown in SEQ ID NO. 1 to SEQ ID NO. 5; a skin sample can be directly collected and added into an LAMP reaction reagent integrated in the micro-fluidic chip, the detection process is automatically completed by a full-automatic detection instrument, a spectrum is analyzed through a spectrum sensor, comprehensive judgment is conducted on collected spectrum signals through an AI technology, full-automatic operation is achieved, the detection time can be controlled within one hour, and the detection efficiency is greatly improved. The kit realizes instant diagnosis, does not need professionals and laboratory environments, is portable and light, can be applied to instant detection in scenes such as pet hospitals on site, adopts an innovative LAMP test method, does not need nucleic acid extraction, and realizes sample outcome.

The invention relates to the technical field of animal histological analysis technology and skin appendage application, in particular to a goose skin hair follicle microsection manufacturing and evaluating method, which specifically comprises the following steps: selecting back skin of a Sanhua goose, avoiding dehairing injury, cutting a 5 * 5mm full-thickness skin sample by using a scalpel, and taking out the full-thickness skin sample; a sample is immediately put into 4% paraformaldehyde to be fixed for 24 hours, stepped ethanol dehydration is adopted, tissue brittleness is reduced, paraffin embedding optimization is adopted, so that a hair follicle structure slice is clear in layer, the method is easy and convenient to operate, tissue treatment procedures do not need to be optimized, it is guaranteed that the thickness of the hair follicle slice is more uniform, and the quality of the hair follicle slice is improved. The number of primary hair follicles and hair follicles is uniform, recording is accurate, hair follicle slice observation and number analysis are facilitated, and the method plays an important role in goose breeding and down feather quality evaluation.

The invention relates to a skin in-vivo confocal imaging image brightness adaptive adjusting device and method, and the method comprises the steps: employing a scanning imaging device to emit illumination light to a skin sample, and carrying out the point-by-point scanning imaging of the skin sample through a micro-electromechanical system in a point-by-point scanning mode; and the brightness of the illumination light is adaptively adjusted in an iterative mode through the brightness adjusting device. In the iteration process, the average signal intensity of the electric signals of the obtained imaging result in the sampling period and the number of the pixels with the signal intensity higher than the reference value are substituted into an empirical formula, an adjusting parameter value for adjusting the brightness of the illumination light in the next step is obtained, and then the brightness adjustment in the next step is carried out. Therefore, according to the self-adaptive adjusting device disclosed by the invention, the light emitting power of the light source does not need to be frequently and manually adjusted or the signal dynamic range in a software system does not need to be manually changed, special image processing is not needed, and the sample detection efficiency and the imaging effect can be improved.

This invention belongs to the field of biodetection technology, specifically disclosing a novel skin typing method, biomarkers based on skin surface metabolomics typing, and their applications. Based on population-level skin surface metabolite measurement data and through multi-dimensional scientific analysis, this application ultimately discovered that the skin samples of the subjects formed two distinct clusters. This demonstrates that differences in metabolomics clusters based on skin typing objectively exist. Furthermore, the two distinct clusters based on the aforementioned skin surface metabolomics data are named "Metabolic Type 1" or "Metabolic Type 2." Simultaneously, these two typing methods also exhibit differences in various skin condition indicators (e.g., sebum levels, actual age, wrinkles, freckles, etc.). By using the typing method provided in this application to study skin surface metabolomics substances, one or more metabolites can be used as biomarkers to identify the specific skin typing level and guide adjustments to the desired skin condition. This application overcomes the limitations of existing methods that define skin typing solely based on dry, oily, etc., providing more comprehensive guidance for practice.

The invention provides a skin cutting device, and belongs to the technical field of medical equipment.The skin cutting device comprises a shell, a linear motion mechanism and a tool bit, and the shell is provided with a containing space with a first opening; the linear motion mechanism is installed in the containing space, and the motion end of the linear motion mechanism can do reciprocating linear motion in the direction pointing to the first opening. The tool bit is detachably connected to the moving end from the first opening, and the tool bit can be driven by the moving end to do reciprocating rectilinear motion so as to cut the skin. According to the skin cutting device provided by the invention, compared with bare-handed skin cutting, a medical worker can conveniently and quickly cut the skin of a patient through the skin cutting device, so that the operation efficiency is improved, and the pain of the patient is effectively reduced; by reasonably designing the specification and size of the cutter head, a skin sample with standard size and thickness can be cut, and the inspection precision of skin biopsy is improved.

The present invention relates to a system for collecting and storing a skin sample of a test subject, the system comprising a collection device comprising an engaging segment, said engaging segment configured to collect the skin sample from the test subject; and a cartridge configured to receive the skin sample after collection The present invention also relates to a corresponding method and use of the system.

Provided herein are methods for diagnosis and treatment of chronic hand eczema (CHE) using skin surface biomarkers. The methods include obtaining from a subject skin samples, detecting certain biomarkers, determining the levels of the biomarkers, comparing the levels to reference levels, and determining whether the subject has CHE.

A computer implemented method for use in conjunction with a computing device, system, network, and cloud with touch screen two dimension display or augmented / mixed reality three dimension display comprising: obtaining, analyzing and detecting user blood, saliva, hair, urine, stool, fingernail, height, weight and skin sampling analysis chemistry data, mapping the blood, saliva, hair, urine, stool, fingernail, height, weight and skin data into a database associated with a specific user, applying the data with optimization equations, mapping equations to food and beverage chemistry, scoring or ranking a plurality of optimized results such that a user may order food and beverage from a food / beverage distribution point or have food / beverage delivered to the user which has been specifically optimized for their specific biochemistry characteristic target ranges. The method is particularly useful in enhancing online internet search engine results.

The utility model relates to the technical field of skin clamping, and discloses a skin clamping device for a drug transdermal tester, which comprises a mounting seat, a plurality of mounting holes are formed in the top of the mounting seat, a plurality of positioning grooves are formed in the top of the mounting seat, springs are fixedly connected to the bottom ends of the interiors of the mounting holes, and the springs are arranged in the positioning grooves. Protective pads are fixedly connected to the tops of the multiple springs, supporting columns are slidably connected to the interiors of the multiple mounting holes, clamping blocks are fixedly connected to the bottom ends of the front sides and the rear sides of the multiple supporting columns, threaded rods are fixedly connected to the tops of the multiple supporting columns, and two chucks are slidably connected to the outer walls of the multiple threaded rods; the tops of the outer walls of the multiple threaded rods are in threaded connection with nuts. According to the skin sample clamping device, the supporting columns are installed at different positions to adapt to skin samples of different specifications, the clamping force is adjusted through the nuts, the effect that the device is convenient to detach for cleaning and maintenance is achieved, and the service life of the device is prolonged.

The invention provides a Parkinson's disease rapid RT-QuIC diagnostic kit based on A30P mutant alpha-synuclein, and belongs to the technical field of detection kits. The specific A30P mutant protein is used as a reaction substrate, and rapid and high-sensitivity amplification detection can be carried out on trace misfolded alpha-synuclein in a skin sample through an optimized RT-QuIC system. The kit has the characteristics of short detection period and convenience in operation, provides a new tool for molecular diagnosis of Parkinson's disease, is suitable for definite diagnosis of clinical PD suspected cases, and can also be used for screening high-risk groups. The kit has the advantages of mature preparation process, easily available raw materials, easiness in industrial production and large-scale popularization, and important clinical application value.

The invention provides a preparation method and a cryopreservation and recovery method of an immortalized Yangtze globefish lipidated keratinocyte line, and belongs to the technical field of animal cell culture. According to the invention, a set of method for preparing an immortalized Yangtze River globefish lipidated keratinocyte cell line in vitro under laboratory conditions is established by utilizing fresh skin samples of Yangtze River globefish and recent dead individuals or living bodies obtained from water field investigation, and a suitable culture, passage and recovery method is explored and finally invented based on the cell line. According to the present invention, the immortalized Yangtze River globefish lipidated keratinocyte line is successfully prepared, and by using the immortalized Yangtze River globefish lipidated keratinocyte amplification culture medium, the immortalized Yangtze River globefish lipidated keratinocytes can normally adhere to the wall to grow; the cryopreservation liquid and the recovery culture medium for the immortalized Yangtze River globefish lipidated keratinocytes can improve the survival rate of the immortalized Yangtze River globefish lipidated keratinocytes.

The invention provides a skin type detection method, a skin type detection device and a computer readable storage medium. The skin detection method comprises the following steps: acquiring spectral image data of a skin sample and standard instrument detection data; preprocessing the spectral image data of the skin sample; constructing a skin detection model according to the standard instrument detection data and the preprocessed skin sample spectral image data; acquiring spectral image data of the skin to be detected; and inputting the spectral image data of the skin to be detected into the skin detection model, and outputting a multi-dimensional skin detection result. According to the skin type detection method, deep and multi-dimensional detection can be accurately, efficiently and reliably carried out on the real skin type, non-contact detection and overall or local detection are supported, and the user experience can be improved.

The application belongs to the technical field of medical diagnosis, and particularly relates to application of Abiotrophia and related metabolites in preparation of a Kawasaki Disease (KD) diagnostic kit. The metabolites are 1,2-dieicosenoyl-sn-glycero-3-phosphocholine and 1,3-dicaffeoylquinic acid. By detecting the levels of the above markers in the skin samples of subjects, non-invasive auxiliary diagnosis of Kawasaki Disease is achieved, and the problem of poor compliance of existing blood tests is solved.

An apparatus for obtaining images for use in determining one or more properties of skin of a subject, the apparatus comprising an image sensor unit for generating an image of a skin sample, wherein the skin sample corresponds to an area of skin of the subject within a field of view, FOV, of the image sensor unit when the skin of the subject is spaced a predetermined working distance from the image sensor unit; a first light arrangement comprising one or more light sources for illuminating the skin of the subject; and a second light arrangement comprising one or more light sources for illuminating the skin of the subject. The one or more light sources in the first light arrangement are spaced from the image sensor unit and arranged such that light emitted by the light sources in the first light arrangement that is specularly reflected by a skin sample at the predetermined working distance is incident on the image sensor unit, and the one or more light sources in the second light arrangement are spaced from the image sensor unit and arranged such that light emitted by the light sources in the second light arrangement that is specularly reflected by a skin sample at the predetermined working distance is not incident on the image sensor unit.

The invention relates to a distillation learning method and device for a skin problem segmentation model, equipment and a medium. The method comprises the following steps: acquiring a skin sample image and an image labeling result corresponding to the skin sample image; performing skin problem region segmentation on the skin sample image through a teacher model to obtain a first output result; performing skin problem region segmentation on the skin sample image through a student model to obtain a second output result; determining distillation loss according to the first output result, the second output result and the image labeling result; determining segmentation loss according to the second output result and the image annotation result; and based on the distillation loss and the segmentation loss, performing model training on the student model to obtain a trained student model. By adopting the method, the detection precision of the skin problem segmentation model can be improved.

Described herein are personalized skin care compositions including a cultured composition including a milk sample and a skin sample. and optionally an additive. Also described herein are kits and methods for making personalized skin care compositions. Also described herein are methods of treating or preventing skin conditions using personalized skin care compositions.

The utility model discloses a skin biopsy sampling tool, which relates to the technical field of medical treatment, and comprises a tool main body, two cutting plates fixedly connected to the lower end of the main body and symmetrically arranged, and sampling tweezers, the lower end of the main body is provided with a mounting groove, the upper end of the sampling tweezers is mounted in the mounting groove, and the lower end of the sampling tweezers extends out of the lower end of the main body. The extending end of the sampling tweezers and the two cutting plates are combined into an annular structure, a limiting column used for limiting the sampling tweezers is fixedly installed in the installation groove, the annular structure formed by the sampling tweezers and the cutting plates is rotatably inserted into the skin sampling position, the two sides of the sampling tweezers are pressed, the sampling tweezers clamp a skin sample, and then sampling tissue can be pulled out from the skin surface. According to the skin biopsy sampling tool, the combination of the punching sampler and the tweezers is completed, and the problems that when an existing skin biopsy sampling tool is used for sampling, the punching sampler and the tweezers need to be alternately used, the operation time is prolonged, and meanwhile the operation complexity is increased are solved.