191 results about "Senescence" patented technology

Metastatic uveal melanomas are highly resistant to all existing treatments. To identify actionable vulnerabilities, the inventors conducted a CRISPR-Cas9 knockout screen using a library composed of chromatin remodelers. They revealed that the histone H3 methyltransferase SETDB1 plays a critical role in metastatic uveal melanoma cell proliferation and survival. Functionally, SETDB1 knockdown triggers decreased expression of genes related to replication and cell cycle and promotes growth arrest associated with increased markers for DNA damage and senescence entry. Using pre-clinical model, they further demonstrated that anti-SETDB1 therapy tumor growth in vivo. The inventors identify SETDB1 as a new relevant therapeutic target for the treatment of metastatic uveal melanomas. The present invention relates to a method for treating uveal melanoma in a subject in need thereof comprising a step of administering said subject with a therapeutically effective amount of SETDB1 inhibitor.

The present application relates to a system for assessing the degree of senescence, which assesses the degree of senescence of a cell by calculating a score of a cell metabolome state by taking into account metabonomics data of the cell. The system is especially suitable for cosmetic efficacy evaluation, anti-aging drug screening and other scenes, can shorten the research and development period in practical application, and has significant industrial application value.

The invention relates to a Saccharomyces cerevisiae C16I09 strain which is preserved in the China Center for Type Culture Collection on May 21, 2025, and the preservation number of the Saccharomyces cerevisiae C16I09 strain is CCTCC (China Center for Type Culture Collection) No. M20251150. The strain disclosed by the invention has good oxidation resistance and can be applied to the fields of skin care products, health care products, anti-aging foods and the like. Especially, extracellular fermentation broth of the strain, namely a fermentation product or a fermentation extract of the strain, has excellent oxidation resistance, can scavenge free radicals and relieve oxidative damage, has the effects of protecting skin, resisting inflammation, delaying senescence and the like, and can be applied to the fields of skin care products, health care products, anti-aging foods and the like as a natural anti-aging agent.

The invention relates to the technical field of collagen intelligent monitoring, and particularly discloses an intelligent collagen monitoring method and system integrated with a microsensor. Enzyme response hydrogel with switchable states is anchored to the skin through a microneedle array, a high-frequency acoustic impedance analysis module is used for continuously probing the acoustic impedance change of the hydrogel, and the collagen monitoring accuracy is improved. Identifying a collagen degradation event; once collagen degradation is detected, the system activates the micro-regeneration unit, a cleaning solution and a regeneration solution are conveyed through a microfluidic channel, a broken peptide chain is removed, a hydrogel cross-linked network is reconstructed, and functional regeneration of the sensing unit is realized; the system further comprises a regeneration state confirmation and cycle reset mechanism, and the reliability of each monitoring cycle and the consistency of data references are ensured. According to the method, the limitation of a traditional monitoring method is overcome, multiple times of recycling of a single sensor is achieved, the credibility and consistency of long-term monitoring data are improved, and the method has important significance on skin health management and senescence research.

The invention discloses an exosome as well as a preparation method and application thereof, and belongs to the technical field of exosomes. The preparation method comprises the following steps: step 1, intermittently immersing and culturing a sterile tissue to obtain a concentrated solution of the exosome I; step 2, collecting the cultured sterile tissues, and performing ultrasonic-assisted extraction to obtain a purified crude extract II; and 3, purifying the concentrated solution obtained in the step 1 and the purified crude extract 2 obtained in the step 2 by size exclusion chromatography to obtain the pure exosome. The exosome obtained by the invention is applied to preparation of cosmetics, health-care foods or medicines for inhibiting skin cell senescence. According to the preparation method disclosed by the invention, impure proteins and non-target particles are effectively removed, the purity of the exosome is improved, the structural integrity of the exosome is kept, false exosome impurities generated by a traditional crushing method are avoided, the yield of the exosome is improved by 3-5 times, and a reliable material basis is provided for subsequent functional research of the exosome.

The invention discloses a pharmaceutical composition with skin repairing, anti-inflammatory and anti-aging effects as well as a preparation method and application of the pharmaceutical composition. The pharmaceutical composition mainly comprises a stem cell exosome and a fusion polypeptide-single domain antibody conjugate, and can also comprise proline and pharmaceutically acceptable auxiliary materials. The amino acid sequence of the fusion polypeptide is as shown in SEQ ID NO.1, and the fusion polypeptide can specifically target skin cells; the single-domain antibody is an anti-IL-6 single-domain antibody, the amino acid sequence of the single-domain antibody is shown as SEQ ID NO.2, and the single-domain antibody can efficiently inhibit inflammatory factors. Experiments show that through the synergistic effect of all the components, the composition remarkably promotes proliferation of skin fibroblasts and keratinocytes, inhibits secretion of inflammatory factors such as IL-6 and TNF-alpha, promotes synthesis of collagen, effectively improves skin aging and inflammation states and has wide application prospects in the fields of skin repair, inflammation resistance and senescence resistance.

The invention discloses a composition for improving immunity and delaying senescence and a preparation method thereof. Belongs to the technical field of functional health. The composition comprises the following components in parts by mass: 5-50 parts of collagen, 10-80 parts of paeonia ostii tea, 1-20 parts of an antioxidant, 3-10 parts of an intestinal microecology regulator and 1-3 parts of an energy metabolism and nerve regulator. According to the invention, through multi-system cooperation and multi-target intervention, dual effects of immune enhancement and senescence delay are realized. Through scientific compounding and a special process, the product has the advantages of stable components, uniform activity and good solubility. Animal experiments and theoretical models show that immune organs can be effectively protected, accumulation of aging markers is reduced, and a solution is provided for development of comprehensive anti-aging health products.

Cardiomyopathies are heart muscle disorders which represent a heterogeneous group of diseases that often lead to progressive heart failure with significant morbidity and mortality. Here, the inventors show that neutralization of acyl CoA binding protein (ACBP) / diazepam binding inhibitor (DBI) reduces the deleterious effect of the Vehicle anthracycline doxombicin on cardiac function. Since anthracycline-induced heart failure and anthracycline-induced senescence are models of accelerated cardiac aging, it is also plausible to use ACBP / DBI inhibition or neutralization as a method to prevent or treat aging of the cardiovascular system. The inventors also found that genetic and pharmacological interventions to deplete ACBP / DBI efficiently improves cardiac dysfunction associated with experimental HFpEF, a condition associated with aging, hypertension and obesity. Accordingly, the present disclosure relates to methods for the treatment of cardiac dysfunction comprising neutralization of ACBP / DBI.

The invention discloses a fresh-keeping storage method of plum fruits, which is characterized in that a compact network structure of a shellac-pullulan-gellan gum basic composition effectively obstructs invasion of external microorganisms, and significantly slows down loss of water in the fruits, so that the plump appearance and hardness of the fruits are maintained, and the fruit quality is improved. The siRNA and the AVG wrapped by the lipidosome accurately interfere with the ripening and senescence process in the fruit from the molecular level, the siRNA specifically silences the gene expression of cell wall degradation key enzyme and delays the softening process of the fruit, and the AVG effectively blocks the biosynthesis pathway of ethylene and inhibits the start of an after-ripening signal, so that the ripening and senescence process of the fruit is delayed. The external formed physical film provides a stable microenvironment for internal bioactive molecules, protects the internal bioactive molecules from being degraded and prolongs the action time, and the internal molecular regulation delays the disintegration of cell structures, so that the external film can be attached more enduringly and exerts a barrier function, and the maintenance of the fruit quality is realized under a refrigeration condition.

The invention relates to a mesenchymal stem cell serum-free culture medium with limited chemical components as well as application and a method thereof, the serum-free culture medium is completely serum-free and has definite components: all additives are known chemical substances and meet the production requirements of cGMP (Complementary Good Manufacturing Practice); telomere shortening can be obviously delayed; replicative senescence is slowed down by activating TERT expression; the multiplication capacity can also be enhanced; the P10 generation cells still have the active division capacity; multidirectional differentiation is supported; the osteogenic differentiation efficiency is superior to that of a traditional serum-containing system; and the method is suitable for industrial production and clinical transformation.

The application discloses application of falcarinol-7-methyl ether and derivatives thereof in preparation of medicines and functional foods for delaying aging or immune regulation. In the application, the falcarinol-7-methyl ether and derivatives thereof are derived from falcarinol-7-methyl ether, and can effectively delay replicative senescence of mesenchymal stem cells, inhibit expression of aging-related markers of mouse liver and lung induced by adriamycin, improve grip strength of the mouse, enhance muscle function, and promote proliferation of T cells separated from human peripheral blood mononuclear cells, and have an immune regulation effect. Therefore, the falcarinol-7-methyl ether and derivatives thereof have good effects of improving telomerase activity, anti-aging and immune regulation, and can be developed into anti-aging or immune regulation products.

The application discloses a novel AIE photosensitizer and a preparation method and application thereof. The AIE photosensitizer of the application comprises TDQ shown in the following structural formula, has superior photo-thermal conversion performance and photodynamic performance, and can be used for preparing a tumor diagnosis reagent or a tumor treatment drug. The co-loading liposome of TDQ and a senescence-inducing molecule (Ali) can be used as a multi-modal light diagnosis and treatment agent to realize efficient delivery of drugs to tumor tissues, and the photo-thermal therapy and the photodynamic therapy based on TDQ not only induce cell apoptosis through oxidative stress and mitochondrial dysfunction, but also trigger cancer cell senescence and promote the secretion of SASPs, so that the anti-tumor immunity is significantly enhanced, and finally the growth of primary tumors, tumor recurrence and re-metastasis are inhibited. The material of the application has simple preparation process, low cost, and is suitable for scale production and clinical medical use.

A compound, or a salt or solvate thereof, according to formula I as defined herein and pharmaceutical composition comprising said compound. Compounds of formula I are capable of detecting senescence. As such, these compounds are useful in methods of detecting or imaging senescent cells or extracts thereof or senescence in a subject or a biological sample taken from a subject.

The invention provides an immortalized livestock precursor fat cell line and a preparation method thereof, and belongs to the technical field of biology. The invention provides a preparation method of an immortalized livestock precursor adipocyte line, which takes adipose tissues of livestock larvae as a cell source, the cell source contains a small amount of mature adipocytes, but a main cell population is precursor adipocytes, so that the purity of a starting material for subsequent immortalization is ensured; after immortalized induction, the monoclonal cell strain is screened by using a limited dilution method, the immortalized monoclonal cell strain still has the capability of being differentiated into mature adipocytes and forming lipid droplets after induced differentiation treatment, and the immortalized cell line can keep normal cell morphology and overcome replicative senescence. The immortalized cell strain disclosed by the invention can be efficiently differentiated into fat cells with mature functions after being induced, and has normal lipid synthesis and storage capabilities.

The application discloses application of a novel peptide UHP in preparation of a medicine for treating and / or preventing delayed aging, and belongs to the technical field of biological medicines.The novel peptide UHP is composed of 37 L-type amino acids including a transmembrane peptide HIV-TAT.It is proved by cell experiments that the novel peptide UHP significantly improves the aging phenotype of a HGPS cell model simulated by overexpression of progerin and a replicative senescent human normal fibroblast cell, reduces the SA-beta-gal positive cell proportion and the expression of SASP factors, and increases the expression levels of cell proliferation markers Ki67, Lamin B1 and Cyclin A2.It is proved by animal experiments that the novel peptide UHP can improve the progeria phenotype, body weight and exercise capacity of a progeria mouse, relieve fibrosis in multiple tissues, improve the skin aging phenotype, and prolong the survival period of the progeria mouse, thereby providing a new medicine selection for clinical treatment of aging-related diseases.

The present invention relates to compounds useful for visualizing cell senescence in vitro and in vivo, the preparation of said compounds and their use. In particular, the present invention pertains to novel glycosides for use as senescence tracers in vitro and in vivo.

This invention relates to the field of tumor molecular biology, specifically providing the application of targeting the ISG15 protein in chemotherapy sensitization drugs and prognostic assessment for B-cell lymphoma. This invention also provides the use of the ISG15 protein as a biomarker in the preparation of prognostic assessment products for B-cell lymphoma. This invention effectively reverses chemotherapy-induced senescence-related drug resistance and restores the chemosensitivity of tumor cells by inhibiting the ISG15 pathway; simultaneously, the ISG15 expression level can serve as a biomarker for prognostic assessment of B-cell lymphoma. This invention provides a new technical solution for overcoming chemotherapy resistance in B-cell lymphoma and assessing patient prognosis.

The invention relates to a gRNA of a targeted non-human animal Zmpste24 gene, a preparation method of a Zmpste24 gene knockout non-human animal model and a phenotype identification method. The gRNA comprises a nucleotide sequence which is complementary with a second exon region of the non-human animal Zmpste24 gene; the target sequence of the second exon region of the non-human animal Zmpste24 gene is as shown in SEQ ID NO: 4; the gRNA comprises gRNA1 as shown in SEQ ID NO: 1 and gRNA3 as shown in SEQ ID NO: 3, and the molar ratio of the gRNA1 to the gRNA3 is (1-5): (1-5). The Zmpste24 gene knockout non-human animal model prepared by the invention shows a remarkable senescence phenotype at the age of 12 weeks, and has the advantages of remarkable phenotype, good reproducibility, short experimental period and the like. The multi-dimensional phenotype identification method established by the invention can comprehensively evaluate the influence of gene knockout on the aging process.

The invention relates to the technical field of biotechnology and animal genetic breeding, and aims at providing application of an SLC2A9 gene in identifying the chicken ovary state. The second purpose of the invention is to provide an application of the SLC2A9 gene or the expression product thereof as a marker in identifying or evaluating the follicular atresia degree of chicken. The third purpose of the invention is to provide the application of the SLC2A9 gene or the expression product of the SLC2A9 gene as a marker in identifying or evaluating the senescence state of the chicken ovary. The invention finds that the SLC2A9 gene or the expression product thereof can be used as a specific biomarker for evaluating the chicken ovarian development state, follicular atresia degree and ovarian aging degree. By detecting the expression level, the objective and quantitative evaluation of the ovarian physiological status of the individual chicken can be realized, and the limitation of only depending on external phenotype observation is overcome.

The invention relates to an opto-acoustic-ultrasonic multi-mode bone metabolism and senescence noninvasive detection method and device, and the method comprises the following steps: respectively collecting an ultrasonic signal and a multi-wavelength opto-acoustic signal in a detection period; calculating the ultrasonic sound velocity and the broadband ultrasonic attenuation coefficient of the to-be-detected bone based on the ultrasonic signal, comparing the ultrasonic sound velocity and the broadband ultrasonic attenuation coefficient with the data of the healthy bone, and preliminarily judging the health degree of bone metabolism; based on the ultrasonic sound velocity and the broadband ultrasonic attenuation coefficient of the to-be-detected bone, performing ultrasonic attenuation compensation on a bone tissue part in the photoacoustic signal; fitting the opto-acoustic signal subjected to ultrasonic attenuation compensation corresponding to each wavelength into a transmission equation of a macroscopic homogeneous medium, calculating to obtain a relative light absorption coefficient of each wavelength, and mapping the relative light absorption coefficient and the corresponding wavelength to obtain a relative light absorption spectrum of the to-be-detected skeleton; and performing linear fitting on the relative light absorption spectrum and the main component spectrum in the skeleton, decoupling related photoacoustic parameters of the chemical component content, and further judging the skeleton metabolism and aging degree. Compared with the prior art, the method has the advantages of being low in detection complexity, non-invasive, non-radiative, capable of comprehensively obtaining physical microstructures and biochemical information related to bone metabolism and aging and the like.

The application provides an application of scEMC10 in treatment and diagnosis of brain vascular endothelial cell aging, and relates to the technical field of biomedicine. The inventor finds that scEMC10 deficiency leads to brain microvascular endothelial cell aging; brain microvascular endothelial cells treated by scEMC10 can down-regulate beta-galactosidase, SASP and classic aging markers related to aging; after old mice and 5XFAD model mice of Alzheimer's disease are supplemented with scEmc10, the movement coordination ability and spatial cognitive ability of the mice can be obviously improved, and the relative content of the Parkinson's disease marker, phosphorylated alpha-synuclein, of the old mice can be obviously reduced, and the content of the tyrosine hydroxylase phosphorylation activity form can be increased. Therefore, scEMC10 can be used for treatment of brain microvascular endothelial cell aging, improvement of Alzheimer's disease and Parkinson's disease, and detection of brain microvascular endothelial cell aging as a marker.

Provided is a combination of at least two of creatine, bifida ferment lysate and sechium edule fruit extract to repair DNA damage to the skin caused by light in the range 280-1400 nm. These agents are combined with UV filters and optionally one or more blue light and / or one or more IR reflector(s) in cosmetic compositions used to prevent sun-induced damage to the skin, in particular to prevent senolytic or senomorphic senescence and / or appearance of the visible signs of skin aging, in particular one or more of fine lines, wrinkles, age spots, skin dryness, thinning of the skin and loss of elasticity.

The invention discloses application of a targeted stimulating molecule in preparation of drugs, health care products and cosmetics for preventing and treating natural aging, and relates to the field of biological medicines. The molecule provided by the invention can mediate the interaction between senescence cells and immune cells, enhance the endogenous immune function, and achieve the purposes of effectively removing senescence cells and improving senescence-related diseases. One end of the functional molecule for targeted senescence cell immune sensitization targets senescence cell membrane molecules, glutamic acid is marked on the surfaces of senescence cells, coupling between endogenous immune cells and the senescence cells is mediated, and adhesion and removal of the senescence cells by the immune cells are promoted. The molecule can regulate the immune microenvironment of senescent tissues, effectively reduce the number of positive senescent cells of senescence-related beta-galactosidase, improve the natural senescence phenotype of an organism and recover the phenotype and function of senescent skin.

The invention is concerned with the cosmetic use of a composition comprising an extract of Saccharomyces cerevisiae for modulating exosome content, thereby preventing aging and / or senescence, in particular intrinsic and / or extrinsic dermal aging.

A compound, or a salt or solvate thereof, according to formula I as defined herein and pharmaceutical composition comprising said compound. Compounds of formula I are capable of detecting senescence. As such, these compounds are useful in methods of detecting or imaging senescent cells or extracts thereof or senescence in a subject or a biological sample taken from a subject.

The invention provides application of a sodium-glucose co-transporter 2 inhibitor in preparation of a medicine for preventing or treating senescence. In an aging animal model, it is found that the sodium-glucose co-transporter 2 inhibitor empagliflozin can improve heart aging, enhance heart functions caused by aging and increase the ejection fraction of left ventricle; meanwhile, the memory function caused by senescence can be improved, and the expression levels of senescence factors p16, p21 and p53 are improved. The sodium-glucose co-transporter 2 inhibitor empagliflozin has a prospect of being developed as an anti-aging drug.

The present disclosure relates to enhancing nitrogen fixation in legumes grown under high nitrate or nitrate stress conditions. In particular, the present disclosure relates to genetically modified plants with altered levels or expression of FUN or downstream targets of FUN, as well as methods for producing and growing the same. The present disclosure also relates to nodule senescence regulated by FUN and its downstream targets, and the regulation of FUN activity or expression by cellular zinc.

The invention provides an application of OICR-9429 or a pharmaceutically acceptable salt thereof in preparation of a product for improving senescence indications. The OICR-9429 provided by the invention shows multiple beneficial technical effects in the aspect of improving senescence-related indications, not only can effectively relieve inflammatory response caused by pyroptosis in the senescence process, but also can improve the immune function and anti-infection ability of senescence organisms. The OICR-9429 also has a remarkable treatment effect on sepsis, the increase of the inflammation level caused by infection is inhibited, the survival rate of old patients is increased, and the prognosis is improved. Long-term intervention of the OICR-9429 can continuously relieve the chronic inflammation state of an aging body, increase the muscle mass, enhance the exercise ability, prolong the life and reduce various lesions accompanied by aging. The invention lays a foundation for developing products for comprehensively intervening aging and related function decline, and has important application value in the aspect of improving the life quality of old people.

The application belongs to the field of fresh flower preservation and relates to a SSS polypeptide, a preservative and application. grf6 and grf8 The mutant has the phenotype of early leaf senescence. A small piece of amino acid of the JID region of the MYC2 protein is prepared into a polypeptide powder, dissolved in ultrapure water to prepare a polypeptide water agent, and sprayed on the surface of the flower. The research of the application shows that spraying the polypeptide reagent can prolong the flowering period of fresh flowers, delay senescence, slow down the drooping of flowers, and slow down the degree of wilting of flowers, thereby achieving the effect of fresh flower preservation. The polypeptide fresh flower preservative has the advantages of safety, non-toxicity, simple operation and obvious effect, and has good application prospect.

The invention discloses a lactobacillus reuteri source extracellular vesicle for preventing and relieving senescence syndrome by relieving immune cell senescence, and belongs to the technical field of microorganisms and the technical field of medicines. The extracellular vesicle provided by the invention can improve learning, memory and cognitive abilities; the muscle strength capability is enhanced; the exercise coordination ability is improved; the motion balance capability is enhanced; the blood inflammatory factor level is obviously reduced, and the anti-inflammatory factor level is improved; the enzyme activity of the senescence-related enzyme SA-beta-Gal in the brain tissue is obviously reduced; the expression levels of brain-derived neurotrophic factors and neurotransmitters are obviously up-regulated; the collagen content and the collagen synthesis capability in skin tissues are obviously improved. The lactobacillus reuteri CCFM1471 source extracellular vesicles do not generate toxic and side effects in a host body, can be used for preparing functional foods and / or dietary supplements and related beauty products with the effects of preventing and relieving senescence syndromes, and have huge application prospects.