10results about How to "Improve anti-aging effect" patented technology

The present application provides a rhodiolide composition with a core-shell-shell structure, a preparation method thereof comprising the steps of: 1) EGCG inclusion: adding beta-cyclodextrin and EGCG into water, ultrasonic, forming an inclusion compound; 2) liposome assembly: weighing soybean phospholipid and cholesterol, dissolving in chloroform to form a uniform solution; weighing rhodiolide, dissolving in the uniform solution, rotary evaporation to remove chloroform, then adding the inclusion compound and PBS buffer into the container, hydrating, forming a liposome wrapping rhodiolide and inclusion compound; 3) shell loading: weighing collagen, mixing with chitosan solution uniformly to form a complex; adding the complex into the liposome, the complex is coated on the outer layer of the liposome, centrifugal purification, obtaining a rhodiolide composition with a core-shell-shell structure. The present application belongs to the technical field of cosmetic raw materials, and the rhodiolide composition provided has the advantages of high stability, good transdermal effect, and significant anti-aging effect.

This invention discloses a fusion protein targeting the urokinase-type plasminogen activator receptor and its application in the field of anti-aging. The fusion protein is composed of a hepatocyte growth factor active domain and a humanized anti-uPAR single-domain antibody linked by a flexible linker peptide, and its specific amino acid sequence is SEQ ID NO:3. This fusion protein can specifically bind to uPAR, which is highly expressed on the surface of senescent cells, with intramolar affinity, achieving targeted delivery of the hepatocyte growth factor active domain. In vitro experiments have demonstrated that this protein can significantly reverse the aging phenotype of human fibroblasts, effectively reduce aging-related β-galactosidase activity, p16INK4a protein expression, and interleukin-6 secretion, and promote cell proliferation. In a rapidly aging mouse model, this protein can systematically improve age-related physiological functional decline. The fusion protein of this invention achieves specific targeting and efficient treatment of senescent cells, providing a new solution for the development of anti-aging drugs.

The invention relates to the technical field of functional compositions, in particular to an anti-wrinkle and firming functional composition containing glassine as well as a preparation method and application of the anti-wrinkle and firming functional composition containing glassine. The composition containing the botryine and having the anti-wrinkle and firming effects is prepared from the following components: 3 to 15 parts of an RS configuration botryine solution, 1 to 3 parts of a spherical PDRN compound, 0.1 to 0.5 part of a folium achilleae millefolium extract wrapped by maltodextrin and 0.5 to 1.25 parts of a sodium mannose phosphate solution, the RS-configuration glassine solution comprises R-type glassine and S-type glassine in a mass ratio of (7-8): (2-3); the spherical PDRN compound is prepared from poly (deoxyribonucleotide) (PDRN), hydrolyzed sodium hyaluronate, 1, 2-pentanediol, 1, 2-hexanediol and deionized water; the sodium mannose phosphate solution comprises sodium mannose phosphate, glycerol and water. Through the multi-target multi-layer synergistic effect of all the components, the obvious tightening and wrinkle fading effects superior to those of a single component or a conventional compound are obtained.

The invention relates to the technical field of skin care product raw materials, and particularly discloses an anti-aging composition and a preparation method thereof, the anti-aging composition comprises the following components by mass: 9-10 parts of a composite extract; 4.7 to 5.3 parts of a cell metabolism activator; 0.9 to 1.1 parts of a preservative; 84.6 to 86.4 parts of a solvent; the composite extract is prepared by compounding a dendrobium officinale stem extract, a snow lotus herb extract, a cistanche deserticola extract and a ganoderma spore extract. The anti-aging tea has the advantage of having a better anti-aging effect.

The application provides a preparation method of medicinal liquor and application of the medicinal liquor in preparation of antioxidant and anti-aging products, and relates to the technical field of pharmaceutical preparations. The preparation method comprises the following steps: (1) crushing; (2) extraction; (3) standing and separation; (4) freezing and filtration, so that the medicinal liquor is obtained. The medicinal liquor is obtained by adopting the steps of batch crushing, mixed extraction, standing, freezing and separating supernatant and suspension by using different separation methods to extract traditional Chinese medicine ingredients. The medicinal liquor can better improve the space exploration ability of aging mice caused by D-galactose, reduce malondialdehyde (MDA), improve superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and has significant antioxidant and anti-aging effects.

The invention relates to the technical field of stem cell engineering and anti-aging biology, in particular to a histone modification regulation method based on placenta amnion sub-totipotent stem cells and an anti-aging preparation thereof. The regulation and control method comprises the following steps: separating and purifying healthy full-month caesarean section placenta amniotic membrane tissues, carrying out composite enzymolysis and flow cytometry screening to obtain CD105 < + >, CD73 < + > and CD45 <-> stem cells, and primarily culturing the CD105 < + >, CD73 < + > and CD45 <-> stem cells; the method comprises the following steps: determining histone methyltransferase MLL3 and demethylase JMJD3 as target spots through ChIP-seq, and determining H3K4me3 and H3K27me3 as target modification sites; an MLL3 activator CM-272 with the final concentration of 15 [mu] M and a JMJD3 inhibitor GSK-J4 with the final concentration of 10 [mu] M form a composite regulator, and induction culture is performed for 84 h under the conditions of 37 DEG C and 5% CO2; and carrying out Western Blot verification and screening on the stem cells meeting the standard. According to the anti-aging preparation, regulated stem cells are used as active ingredients, a sodium alginate-gelatin (2: 1) composite carrier, 5% mannitol and 1% human serum albumin protective agent are used as auxiliary materials, and the injection with the pH of 7.2-7.4 is prepared. The method is accurate in regulation and control, and the preparation is remarkable in anti-aging effect and low in immunological rejection risk.

The invention relates to the technical field of health-care food, in particular to application of Chinese wolfberry fruit puree in preparation of an anti-aging preparation. The invention provides application of Chinese wolfberry fruit puree in preparation of an anti-aging preparation. The Chinese wolfberry fruit puree comprises Chinese wolfberry fruits and Chinese wolfberry fruit seed oil extracted from Chinese wolfberry fruit seeds. According to the present invention, the Chinese wolfberry primary pulp can significantly enhance the muscle strength of mice, improve the movement coordination degree and the balance force of the mice, and achieve the effect better than the effect of the single use of the same dose of the Chinese wolfberry aqueous solution, such that the Chinese wolfberry primary pulp has the good anti-aging effect;

The invention discloses a vanadyl sulfate anti-aging composition as well as a preparation method and application thereof, and belongs to the technical field of aging resistance. The vanadyl sulfate anti-aging composition comprises the following active ingredients in parts by mass: 1-10 parts of vanadyl sulfate, 5-20 parts of vitamin E, 2-15 parts of glutathione and / or 1-8 parts of astaxanthin. According to the vanadyl sulfate anti-aging composition disclosed by the invention, the active ingredients are compounded according to a specific proportion, a quaternary active ingredient system with a synergistic effect is constructed, and the active ingredients play roles from different target spots, so that excellent anti-aging performance is shown. Meanwhile, the anti-aging composition can remarkably inhibit the natural oxidation process of vanadyl sulfate and effectively inhibit the generation of toxic + 5-valent vanadium, so that the use safety of the composition is greatly improved, and the chemical stability and bioavailability of the core active component in the long-term storage and use process are also ensured.

The application relates to a preparation method of MMP-13 response nanoparticles loaded with PDRN, and belongs to the technical field of biological medicines. In order to solve the problems of low transdermal efficiency of polydeoxyribonucleotide, poor stability under conventional storage conditions, and the fact that a traditional delivery system cannot distinguish between aging and normal tissues, leading to insufficient concentration at a target site and low bioavailability, the application provides MMP-13 response nanoparticles loaded with PDRN. By introducing a natural and safe response unit and a cross-linking strategy, an innovative full-chain solution and solid data support are provided for the development of the next generation of intelligent anti-aging cosmetics which are precise in targeting, efficient in anti-aging, and excellent in stability.

The present application relates to a kind of lycopene and resveratrol microbead and preparation method thereof, to provide a kind of lycopene and resveratrol microbead capable of stable package, not only improve the solubility of two, and have significant antioxidant effect, can make active ingredient stable storage before use, belong to the skin care product technical field.