52 results about "Telomere" patented technology

Provided herein are methods for determining allele-specific telomere length (ATL) and telomere variant repeat (TVR) sequences from long read sequencing data. In some embodiments, the methods include clustering telomere reads together into individual alleles using their adjacent TVR regions from which allele-specific TL can then be determined.

The invention discloses a Rongchang pig T2T genome assembly method. The method comprises the following steps: 1) collecting and sequencing a sample; 2) genome investigation and assembly; 3) genome annotation; wherein in the sequencing step, three sequencing technical means, namely, a three-generation gene sequencing technology PacBio, Nanopore PromethION 48 short reading and chromatin conception capture (HiC), are adopted, and the Rongchang pig genome is subjected to sequencing and sequence splicing together. The Contig N50 value of the genome is nearly three times that of Sscrofa11.1, and the improvement is mainly embodied in a complex genome region (centromere and telomere regions), so that the genome becomes the most complete genome available at present.

Provided in the present invention are the use of insertion / deletion polymorphism genetic markers in detecting the copy number of human chromosomal subtelomeric regions, a reagent panel for detecting the insertion / deletion polymorphisms genetic markers, and a method for detecting the copy number of human chromosomal subtelomeric regions.

The invention belongs to the technical field of molecular biology, and particularly relates to a standard substance for detecting absolute telomere length and a preparation method and application thereof. The standard substance provided by the invention exists in the form of plasmids, and comprises a telomere standard substance and a single copy gene IFNB1 standard substance. The specific standard substance for detecting the absolute telomere length has good and stable amplification efficiency, and the standard curve stability of the standard substance is high.

This invention discloses a method for calculating stress age based on saliva extraction, belonging to the field of data processing technology. It includes collecting saliva samples and personal data from individuals, including individual characteristics, physiological data, and behavioral data; obtaining telomere detection data from the saliva samples; analyzing the telomere detection data, physiological data, and behavioral data to obtain telomere-related data; and generating a test report based on the telomere-related data. This method achieves the scientific quantification of emotional and psychological stress, and compared to traditional scales, the test results are more scientific and accurate.

The present disclosure relates, according to some embodiments, to telomere resolvases (e.g., TelA variants) having a turnover number over 1.

The present invention relates to a novel compounds for use in treatment or prevention of cancer, in particular to use of said compounds as an inhibitors of interactions between TRF1-TIN2 or TRF2-TIN2 telomeric proteins.

An object of the present invention is to provide a novel use of fermented papaya preparation. A composition of the present invention is a composition for elongating telomeres comprising fermented papaya preparation as an active ingredient.

Disclosed are use of a circRNA molecule as a diagnostic marker in a cell senescence diagnosis kit, and use thereof as a treatment target for cell senescence. The present invention finds that circHERC1 is lowly expressed in both cell models of natural senescence and induced senescence. Overexpression of circHERC1 in endothelial cells can improve the proliferation capacity of the endothelial cells, effectively extend the telomere length of the cells, activate telomerase, reduce the β-galactosidase positive rate of the cells, and reduce the expression of all senescence-associated proteins. Introduction of exogenous circHERC1 into mice can effectively delay the senescence of various organs and the shortening of telomeres in aged mice and reduce the β-galactosidase positive rates of various organs of aged mice.

The invention relates to a primary sicca syndrome biomarker. The biomarker is telomere relative length. By detecting the relative length of telomeres in peripheral blood leucocytes and combining a statistical model, the early diagnosis sensitivity and specificity of the primary sicca syndrome are remarkably improved. Compared with a traditional diagnosis method, the disease risk can be predicted before clinical symptoms appear, and therefore early intervention of diseases is achieved.

The invention discloses a hybrid calculation method for generic genome assembly, which is characterized in that based on a hybrid calculation framework guided by de novo assembly and a reference genome, the reference genome depth is integrated into the whole process of generic genome assembly, and the generic genome assembly is obtained by utilizing telomeres of a sibling and / or same species to telomere reference genome and 10-20kbHiFi read length. A high quality replacement long sequence is generated to simulate and / or replace up to millions of bp ONT reads, achieving continuous high precision generic genome assembly with low computational power consumption. According to the invention, the developed hybrid computing framework and algorithm tool are combined with de novo assembly and a hybrid computing tool based on a reference assembly method, so that the existing reference genome from the same or related species can be efficiently utilized, and a high-quality substitutive long sequence can be generated by combining PacBio HiFi read length; the sequences and de novo assembly results are integrated to improve the assembly quality of a group scale data set, and reliable gene information resources are provided for research and application of generic genomes.

The invention relates to the technical field of genomics and microbial science, in particular to a myxobacteria T2T genome assembling method. The assembling method mainly comprises the four steps of global assembling, telomere sequence screening, local sequence clustering screening and second-generation data multi-round correction. According to the method provided by the invention, pollution removal strategies of telomere sequence screening and local sequence clustering screening are newly added, so that the problem that tandem repeat sequences in a myxobacteria genome are complex and short is effectively solved, the analysis of a complex region becomes possible, the integrity and continuity of assembly are improved, and the assembly efficiency is improved. The method does not need fingerprint spectrum, Hi-C technology or optical spectrum sequencing technology, reduces the experiment cost, ensures that all the sequences belonging to myxobacteria are extracted, effectively removes the interference of repetitive sequences and deep pronucleus pollution, enables highly similar repetitive sequence fragments to be reduced and positioned to an accurate genome position, and improves the accuracy of the detection result. The integrity and continuity of the genome are obviously improved.

The invention relates to a high-throughput method for detecting chromosomal aberrations and / or telomere aberrations, using a biological sample of 150 μl to 200 μl.

Provided is a method of preparing at least one ligation product from a sample including a plurality of single-strand nucleic acid fragments, the method including the steps of: (a) ligating a first universal oligonucleotide adaptor to at least one single-strand nucleic acid fragment, wherein the first universal oligonucleotide adaptor is configured for ligating to a 3′ end of individual single-strand nucleic acid fragment; and (b) ligating a second universal oligonucleotide adaptor to the at least one single-strand nucleic acid fragment, wherein the second universal oligonucleotide adaptor is configured for ligating to a 5′ end of individual single-strand nucleic acid fragment, thereby at least one ligation product is formed. In another embodiment, provided is at least one cancer biomarker comprising human telomere sequence with two or more consecutive repeats of nucleotide sequence TTAGGG.

The invention belongs to the technical field of biology, and particularly relates to a method for improving in-vitro mature oocyte telomere length and maturation quality and application of the in-vitro mature oocyte telomere length and the maturation quality. And daphnetin. According to the preparation disclosed by the invention, at least one of the Sema3 type protein and the daphnetin is added, so that the telomere length of an oocyte and an in-vitro embryo is effectively maintained, repaired or prolonged, the maturation quality of the in-vitro oocyte and the development potential and development efficiency of the in-vitro embryo are further remarkably improved, and further, the development potential and development efficiency of the in-vitro embryo are further improved. The application provides a more effective new strategy for in-vitro embryo production of livestock, animal genetic improvement, assisted reproduction technology and the like, and the application prospect is wide.

The application discloses H3F3A beta protein involved in regulating telomere length and application thereof. The amino acid sequence of the H3F3A beta protein is shown in the sequence table SEQ ID NO:1. The application constructs H3F3A beta specific knockout cell lines and H3F3A beta mutant recovery cell lines, finds that compared with a wild type control group, the telomere length of the H3F3A beta specific knockout cell lines is significantly shortened, and cell proliferation is significantly slowed down, the telomere length of the H3F3A beta mutant gene recovery cell lines is significantly lengthened, and cell proliferation is significantly accelerated, proves that H3F3A beta plays a biological role in maintaining the length of telomeres, and has an effective inhibiting effect on the shortening of the length of telomeres by overexpressing H3F3A beta. Since the length of telomeres can directly affect the aging process of the body, therefore, the H3F3A beta and the mutant related genes or the recombinant expression vector provided by the application have the potential for preparing anti-aging drugs.

A method of treating a cancer in a patient includes obtaining a sample from the patient, using a C-circle assay to detect a presence of an alternative lengthening of telomeres (ALT) phenotype in the sample, and administering an effect amount of at least one of PRIMA-1 or APR-246 to the patient.

The application belongs to the technical field of biological medicine, and particularly relates to a composition for improving NAD+ level and a preparation method and application thereof. The composition is prepared by compounding NMN and urolithin A. Through synergistic effect of the two, the problem of limited efficacy of single component is effectively solved. The composition can efficiently improve NAD+ level, prolong telomere length, improve sleep quality under the condition of halving the dosage, improve aging-related indexes from multiple targets and multiple dimensions, and improve product safety and economy, thereby providing a scientific basis for development of related functional products.

The invention discloses a primer pair for evaluating large yellow croaker cell senescence based on relative telomere length and a method thereof, and belongs to the field of molecular genetics. The specific method comprises the following steps: 1) taking DNA of a large yellow croaker cell genome; 2) performing qPCR amplification on the DNA obtained in the step S1 by using a first qPCR amplification system containing a telomere universal primer to obtain a telomere copy number Cttel; (3) carrying out qPCR amplification on the obtained DNA by using a second qPCR amplification system containing the forward primer as shown in SEQ ID No.1 and the reverse primer as shown in SEQ ID No.2, so as to obtain the copy number Ctactb2 of the internal reference single copy gene; and 4) calculating the relative telomere length T / S = 2-[delta] Ct, wherein [delta] Ct = Ctel-Ctactb2. The relative telomere length detection actb2 primer provided by the invention can specifically amplify large yellow croaker DNA, and can realize accurate detection by adopting a trace sample.

The invention relates to casein peptide hydrolysate and application thereof in preparation of drugs for preventing or treating nerve aging, and belongs to the technical field of biological medicines. The casein hydrolysate is obtained by fermenting a casein solution with the probiotic bifidobacterium longum BNCC 185354, and the obtained casein hydrolysate has superoxide anion free radical scavenging capacity, can prolong the telomere length of the hippocampus of a subacute aging model mouse and relieve DNA oxidative damage of the hippocampus of the mouse. The brand-new casein peptide with both brain-accessible activity and biological activity is further screened from casein hydrolysate, free radicals can be removed, nerve cell inflammation can be eliminated, and therefore nerve aging is delayed. The casein peptide and the casein hydrolysate provided by the invention can be applied to preparation of drugs for preventing and / or treating nerve aging, and have good application prospects.

The present application discloses a method and system for filling the gap of genome assembly. The present application filters out the conflicting alignment by processing the preliminary alignment file, and calculates a series of specific scores to evaluate the quality of the alignment, and obtains the sequence corresponding to the optimal gap filling based on the alignment quality. The present application can effectively fill the gap during genome assembly by simple operation without professional technical personnel, and is particularly suitable for telomere-to-telomere level genome assembly. Meanwhile, the present application can greatly reduce the gap filling time and avoid high workload.

This utility model relates to medical diagnostic equipment and in vitro laboratory diagnostics, including microfluidic impedance cytometers for rapid assessment of the white blood cell (WBC) count in whole blood. It can be used in automated systems for calculating biological age based on telomere indices (T / S) and / or associated biomarkers. The technical result is the elimination of biological age (BA) bias arising from interindividual differences in the white blood cell count (proportion of granulocytes and lymphocytes), without the use of a separate blood analyzer, thanks to integrated multi-frequency impedance classification of WBC and automatic transfer of the calculated proportions to the telomere computing circuit (TEL-BA / BA-Dock) for T / S → BA regression correction.The technical result is achieved in that the impedance microcytometer "WBC-Adjust" is made in the form of an embedded (inline) micromodule installed downstream of the qPCR cartridge, and contains a microfluidic channel with a hydrodynamic focusing system, an array of measuring electrodes connected to a multi-frequency impedance meter, an electronic unit for classifying cells according to a set of "size-multi-frequency dielectric profile" features, a communication interface (wired / wireless) for transmitting calculated WBC shares to the TEL-BA / BA-Dock, as well as a controller implementing self-testing protocols, temperature monitoring and synchronization with the main measuring cycle of the telomeric cartridge.

The invention discloses a quantitative analysis method and system for HER2 gene copy number based on a neural network, and relates to the technical field of gene detection, the system comprises a microscopic monitoring module, a laser reflection module, a neural network processing module and a probability adjustment module, the microcosmic monitoring module generates a microcosmic magnified image reflecting a blood sample by adopting receiving and interpretation of an electron microscope, and the laser reflection module adopts characteristics of diffuse reflection laser reflected when artificial laser propagation encounters telomere and centromere of No.17 chromosome; the laser absorption rate change caused by different dyeing intensities in a cell nucleus is measured, the laser absorption rate is evaluated, the neural network processing module combines a processed micro-amplified image with a reflected light analysis result to form an image internal comprehensive analysis result, and the probability adjusting module is used for adjusting the probability according to the reflected light analysis result. The method has the characteristic of high accuracy.

The invention provides a multi-modal screening system and method for candidate patients for cochlear implantation of senile deaf patients. The method comprises the steps that the relative telomere length of leukocyte DNA in peripheral blood sample data of the senile deaf patients and a temporal bone three-dimensional image of the senile deaf patients are obtained; analyzing the temporal bone three-dimensional image to obtain an oval window width and an included angle between a facial nerve level segment and a projia; obtaining an electric evoked compound action potential signal of the auditory nerve of the senile deafness patient under the electric stimulation effect of the preset parameters, and obtaining a filtered electric evoked compound action potential signal; based on the telomere relative length, the oval window width, the included angle and the filtered electric evoked composite action potential signal, candidate patients suitable for cochlear implant implantation are judged; according to the technical scheme provided by the invention, the construction of a comprehensive evaluation system is realized, so that the artificial cochlea implantation fitness of the patient is evaluated more accurately, and the scientificity and reliability of candidate patient screening are improved.

The application relates to the technical field of biological medicine, and particularly discloses a telomere-related gene marker for hepatocellular carcinoma prognosis prediction, a prognosis risk scoring model and application thereof. The application integrates the expression profile of a telomere-related gene in a TCGA-LIHC queue and clinical data, uses consistent clustering analysis to identify two telomere subtypes with significant survival difference, and further uses LASSO-Cox regression to construct a hepatocellular carcinoma prognosis risk scoring model containing 20 characteristic genes. The model shows excellent prediction performance in an internal training set and an external validation set, and can accurately distinguish high-risk and low-risk patients. The risk score is closely related to a tumor immune microenvironment, and high-risk patients show an immune inhibition state, such as reduced CD8+ T cell infiltration, which provides a basis for predicting the response of patients to an immune checkpoint inhibitor.

The present application relates to the field of microbial technology, and particularly relates to the application of AKK bacteria and products thereof in anti-aging and / or anti-oxidation. The preservation number of the AKK bacteria is CGMCC No. 20954, and the strain is preferably used in a pasteurized form. Through three kinds of zebrafish model experiments, it is proved that the strain can effectively improve cell aging, specifically, the telomere length is improved, the expression amount of anti-aging gene spns1 is enhanced, and the symptoms of muscle atrophy and cognitive impairment caused by aging are obviously improved. In particular, the minimum effective amount of the strain in the water body for feeding zebrafish is 250 μg / mL, and the minimum effective amount in the human body is 30 billion AFU per person per day. The present application provides a clear strain source and dosage basis for developing a multi-dimensional and systematic anti-aging functional composition.

The application discloses an aspergillus telomere-to-telomere genome assembly method, device, equipment and storage medium. The method comprises the following steps: using a plurality of sequencing sequence assembly tools to assemble target aspergillus long read genome sequencing data from scratch to obtain a first assembled genome; selecting a first assembled genome meeting a preset condition as an initial assembled genome; integrating other first assembled genomes to fill gaps between repeat regions of the initial assembled genome to obtain a second assembled genome; aligning the long read genome sequencing data to the second assembled genome, identifying abnormal coverage regions and correcting sequences to obtain a third assembled genome; aligning a reference genome to the third assembled genome, connecting and orienting different contigs, and mounting the contigs to chromosomes to obtain a fourth assembled genome; and aligning the long read genome sequencing data and short read sequencing data to the fourth assembled genome for correction to obtain an aspergillus telomere-to-telomere genome assembly result.

The invention discloses a library building method for detecting G-quadruplex structures in oocytes, early embryos and trace cells, and belongs to the technical field of whole genome immunoprecipitation library building. The DNA G-quadruplex structure provided by the invention is a DNA secondary structure which is different from a classic double-helix structure; g-quadruplex structure abnormity can damage telomere stability, genome stability, transcriptional activity and the like; this is an important reason for poor ovum quality and embryonic development retardation. The method provided by the invention can be used for evaluating the quality of the ova of the infertile women and judging reasons causing immaturity of the ova; abnormal enrichment of in-vivo and in-vitro development arrest embryo whole genome G-quadruplex is detected and can be used as an important index for screening reasons causing embryonic development retardation; the method can be used for guiding whether corresponding clinical patients are suitable for a series of assisted reproductive technologies (ART) such as in vitro fertilization-embryo transfer (IVF-ET) and the like.

A composition comprising a recombinant probiotic organism engineered to constitutively express an enzyme involved in maintaining telomere length, wherein the enzyme comprises a telomerase subunit, the genetic modification comprises an operon comprising a gene sequence encoding telomerase having a promoter recognized by the RNA polymerase of the probiotic organism and a genetic modification that induces the secretion of telomerase into the host organism, and the genetic modification of the telomerase gene sequence enhances the entry of the telomerase enzyme into the host organism's cells.

This invention belongs to the field of genomics and bioinformatics technology, specifically relating to a method for multidimensional quality assessment of telomere-to-telomere genomes. The method includes the following steps: preprocessing the genome data to be assessed to obtain preprocessed data; quantitatively assessing multiple core features of the preprocessed data, including accuracy, completeness, continuity, and region specificity; the region specificity includes telomere identification, centromere identification, and collinearity with a reference genome; calculating a comprehensive score based on the weighted results of the quantitative assessment of the core features, and using the comprehensive score to determine the quality of the genome to be assessed. This assessment method is specifically adapted to the core features of T2T genomes, taking into account continuity, completeness, accuracy, and region specificity, thus solving the problem that traditional methods cannot assess T2T-specific features.