80 results about "Telomere" patented technology

The invention discloses a reference-level high-quality genome assembly method, which is characterized in that a compPoFi tool integrates a plurality of high-performance assembly tools, does not need expensive ONT sequencing, and can realize multi-species reference-level high-quality genome assembly only by adopting a second-generation Illumina short read length, a third-generation HiFi (Revio platform, average read length of 25kb) and Hi-C chromatin conformation capture sequencing. The sequencing cost is obviously reduced. In order to solve the problem of genome assembly with a high transposition element ratio, the average read length of a HiFi sequencing library is required to reach 25kb so as to cross most transposition elements. Under the standard, a developed compPoFi tool is utilized, the assembly of the high-quality chromosome scale reference genome of the field worst weed beckmannia syzigachne is completed for the first time, all telomere and centromere regions are identified, and the 2.8 G beckmannia syzigachne genome is assembled to the near telomere to telomere (T2T) level. The invention provides a more economical high-quality genome assembly scheme, and lays a research foundation for understanding rapid adaptation and evolution of weeds under environmental stress.

Provided herein are methods for determining allele-specific telomere length (ATL) and telomere variant repeat (TVR) sequences from long read sequencing data. In some embodiments, the methods include clustering telomere reads together into individual alleles using their adjacent TVR regions from which allele-specific TL can then be determined.

The invention provides a bifidobacterium animalis subspecies HX-BA21, and the preservation number of the bifidobacterium animalis subspecies HX-BA21 is CGMCC (China General Microbiological Culture Collection Center) NO.32958. The invention discloses bifidobacterium animalis subspecies HX-BA21 containing exosome metabiotics and an exosome-containing fermentation compound obtained by fermenting gastrodia elata through the HX-BA21. The fermentation compound comprises a probiotic protective layer (a core layer), an exosome delivery layer (a shell layer) and a prebiotics sustained release layer (a cocoon layer) from inside to outside. The probiotic protection layer comprises a sodium alginate-milk fat globule membrane compound and animal bifidobacterium subsp.animalis; the exosome delivery layer comprises a gelatin-chitosan compound and a gastrodia elata and probiotic mixed exosome; the prebiotic sustained-release layer comprises an octenyl succinic anhydride starch / polyvinyl alcohol compound and inulin. The compound can start cell repair by improving the cell migration rate, up-regulate DNA helicase and telomere related gene expression, delay premature senescence and delay cell senescence caused by D-galactose by adjusting the oxidative stress level.

The invention discloses a Rongchang pig T2T genome assembly method. The method comprises the following steps: 1) collecting and sequencing a sample; 2) genome investigation and assembly; 3) genome annotation; wherein in the sequencing step, three sequencing technical means, namely, a three-generation gene sequencing technology PacBio, Nanopore PromethION 48 short reading and chromatin conception capture (HiC), are adopted, and the Rongchang pig genome is subjected to sequencing and sequence splicing together. The Contig N50 value of the genome is nearly three times that of Sscrofa11.1, and the improvement is mainly embodied in a complex genome region (centromere and telomere regions), so that the genome becomes the most complete genome available at present.

The invention discloses a method for screening an active matter for protecting skin cell telomeres, and relates to the technical field of biotechnology and cosmetic development, and the method is technically characterized by comprising the following steps: a) providing stem cells derived from an exosome which carries at least one microRNA for regulating and controlling a telomere protection factor CTC1; b) acting a candidate active matter on the stem cells, and separating to obtain a treated exosome; the technical effects are as follows: the combination of miR-150-5p and miR-181c is delivered through the exosome, the dual-miRNA coordinated regulation is realized, the limitation of the traditional single-target detection is broken through, the CD63 / CD81 protein on the membrane surface of the exosome can be specifically combined with the skin cell membrane, and the Franz diffusion experiment verifies that the micro-fluidic chip integrates the collagen scaffold and the dynamic shear force.

Provided in the present invention are the use of insertion / deletion polymorphism genetic markers in detecting the copy number of human chromosomal subtelomeric regions, a reagent panel for detecting the insertion / deletion polymorphisms genetic markers, and a method for detecting the copy number of human chromosomal subtelomeric regions.

The invention belongs to the technical field of molecular biology, and particularly relates to a standard substance for detecting absolute telomere length and a preparation method and application thereof. The standard substance provided by the invention exists in the form of plasmids, and comprises a telomere standard substance and a single copy gene IFNB1 standard substance. The specific standard substance for detecting the absolute telomere length has good and stable amplification efficiency, and the standard curve stability of the standard substance is high.

This invention discloses a method for calculating stress age based on saliva extraction, belonging to the field of data processing technology. It includes collecting saliva samples and personal data from individuals, including individual characteristics, physiological data, and behavioral data; obtaining telomere detection data from the saliva samples; analyzing the telomere detection data, physiological data, and behavioral data to obtain telomere-related data; and generating a test report based on the telomere-related data. This method achieves the scientific quantification of emotional and psychological stress, and compared to traditional scales, the test results are more scientific and accurate.

Methods are provided to capture telomere-associated DNA loci from chromatin. Chromatin was prepared from cultured cells by dual crosslinking using formaldehyde and EGS, followed by sonication. Biotinylated PNA probes were hybridized to the telomere DNA sequences, and serine protease was used to release the telomere-associated DNA sequences. The released DNA fragments in the supernatant were purified and subjected to library preparation for next-generation sequencing. The ligation-free chromatin capture and sequencing method enriches specific telomere-associated DNA loci to provide an accurate representation of chromatin interaction. Insights into the chromatin interactions occurring at telomeres are used to investigate the potential role of telomers in aging and age-related diseases.

The present disclosure relates, according to some embodiments, to telomere resolvases (e.g., TelA variants) having a turnover number over 1.

The present invention relates to a novel compounds for use in treatment or prevention of cancer, in particular to use of said compounds as an inhibitors of interactions between TRF1-TIN2 or TRF2-TIN2 telomeric proteins.

The invention relates to the technical field of medical detection, in particular to a chromosome image detection and hardware acceleration method based on a morphological prior fusion network. According to the technical scheme, the chromosome image detection and hardware acceleration method based on the morphological prior fusion network comprises the following steps: S1, extracting brightness distribution and noise distribution of telomeres and arm regions from a chromosome medical image, and generating a normalized initial image through weighted filtering and chromaticity correction; s2, performing multi-stage segmentation on the normalized image based on medical priori, extracting telomere positions, arm length and curvature parameters in combination with a contour tracking algorithm, and generating a region label containing morphological characteristics; s3, dynamic Anchor generation: according to telomere positions, arm length and curvature parameters in the morphological characteristics, generating a candidate frame adapted to a chromosome slender curved structure. According to the method, noise interference is effectively suppressed, microstructure features such as chromosome fracture and bending are reserved, and the contour extraction precision in a complex scene is improved.

An object of the present invention is to provide a novel use of fermented papaya preparation. A composition of the present invention is a composition for elongating telomeres comprising fermented papaya preparation as an active ingredient.

Disclosed are use of a circRNA molecule as a diagnostic marker in a cell senescence diagnosis kit, and use thereof as a treatment target for cell senescence. The present invention finds that circHERC1 is lowly expressed in both cell models of natural senescence and induced senescence. Overexpression of circHERC1 in endothelial cells can improve the proliferation capacity of the endothelial cells, effectively extend the telomere length of the cells, activate telomerase, reduce the β-galactosidase positive rate of the cells, and reduce the expression of all senescence-associated proteins. Introduction of exogenous circHERC1 into mice can effectively delay the senescence of various organs and the shortening of telomeres in aged mice and reduce the β-galactosidase positive rates of various organs of aged mice.

The invention relates to a primary sicca syndrome biomarker. The biomarker is telomere relative length. By detecting the relative length of telomeres in peripheral blood leucocytes and combining a statistical model, the early diagnosis sensitivity and specificity of the primary sicca syndrome are remarkably improved. Compared with a traditional diagnosis method, the disease risk can be predicted before clinical symptoms appear, and therefore early intervention of diseases is achieved.

The invention discloses a hybrid calculation method for generic genome assembly, which is characterized in that based on a hybrid calculation framework guided by de novo assembly and a reference genome, the reference genome depth is integrated into the whole process of generic genome assembly, and the generic genome assembly is obtained by utilizing telomeres of a sibling and / or same species to telomere reference genome and 10-20kbHiFi read length. A high quality replacement long sequence is generated to simulate and / or replace up to millions of bp ONT reads, achieving continuous high precision generic genome assembly with low computational power consumption. According to the invention, the developed hybrid computing framework and algorithm tool are combined with de novo assembly and a hybrid computing tool based on a reference assembly method, so that the existing reference genome from the same or related species can be efficiently utilized, and a high-quality substitutive long sequence can be generated by combining PacBio HiFi read length; the sequences and de novo assembly results are integrated to improve the assembly quality of a group scale data set, and reliable gene information resources are provided for research and application of generic genomes.

The invention relates to the technical field of genomics and microbial science, in particular to a myxobacteria T2T genome assembling method. The assembling method mainly comprises the four steps of global assembling, telomere sequence screening, local sequence clustering screening and second-generation data multi-round correction. According to the method provided by the invention, pollution removal strategies of telomere sequence screening and local sequence clustering screening are newly added, so that the problem that tandem repeat sequences in a myxobacteria genome are complex and short is effectively solved, the analysis of a complex region becomes possible, the integrity and continuity of assembly are improved, and the assembly efficiency is improved. The method does not need fingerprint spectrum, Hi-C technology or optical spectrum sequencing technology, reduces the experiment cost, ensures that all the sequences belonging to myxobacteria are extracted, effectively removes the interference of repetitive sequences and deep pronucleus pollution, enables highly similar repetitive sequence fragments to be reduced and positioned to an accurate genome position, and improves the accuracy of the detection result. The integrity and continuity of the genome are obviously improved.

The invention relates to a high-throughput method for detecting chromosomal aberrations and / or telomere aberrations, using a biological sample of 150 μl to 200 μl.

Provided is a method of preparing at least one ligation product from a sample including a plurality of single-strand nucleic acid fragments, the method including the steps of: (a) ligating a first universal oligonucleotide adaptor to at least one single-strand nucleic acid fragment, wherein the first universal oligonucleotide adaptor is configured for ligating to a 3′ end of individual single-strand nucleic acid fragment; and (b) ligating a second universal oligonucleotide adaptor to the at least one single-strand nucleic acid fragment, wherein the second universal oligonucleotide adaptor is configured for ligating to a 5′ end of individual single-strand nucleic acid fragment, thereby at least one ligation product is formed. In another embodiment, provided is at least one cancer biomarker comprising human telomere sequence with two or more consecutive repeats of nucleotide sequence TTAGGG.

Computer-implemented methods for predicting alternative lengthening of telomeres (ALT) status are described. The computer-implemented methods may comprise, for example, receiving, at one or more processors, targeted sequence read data derived from a sample from a subject; processing, using the one or more processors, the targeted sequence read data to identify one or more genomic features; providing, using the one or more processors, the one or more genomic features as input to a trained model configured to predict an ALT status of the sample based on the one or more genomic features; and outputting, using the one or more processors, the predicted ALT status of the sample.

The invention discloses a toolkit and method for supplementing chromosome telomeres in a T2T genome, and the toolkit comprises a comparison and screening module which is used for comparing a long reading sequencing sequence of a to-be-detected genome with a reference genome, and screening a comparison result to obtain a first out-of-range sequence; the genome assembly and polishing module is used for constructing and polishing a draft genome according to the overlapping relationship among the fragments in the first out-of-range sequence; the new genome generation module is used for comparing the polished draft genome into the original genome to obtain a secondary comparison result, determining start-stop position information of a second out-of-range sequence, extracting a telomere sequence and attaching the telomere sequence to the original genome to obtain a new genome; and the visual output module is used for obtaining a telomere sequence analysis report according to the original genome, the new genome and the specific telomere motif of the corresponding species. According to the method, the existing sequencing data can be fully utilized to improve the assembly integrity of the telomere region, and complete assembly of the telomere region is realized.

The invention relates to the field of microbial products, in particular to application of Lactobacillus kefir Fanghua in preparation of products for prolonging life of animals or increasing telomere length and a composition containing the Lactobacillus kefir Fanghua. According to the present invention, the preservation number of the Lactobacillus kefir Fanghua is CGMCC (China General Microbiological Culture Collection Center) No.27733, and the preservation number of the Lactobacillus kefir Fanghua is CGMCC No.27733; the intervention of the Kefir Lactobacillus brachyphyllum Fanghua or a product containing the strain can significantly increase the telomere length of cells, and significantly prolong the life of caenorhabditis elegans.

The invention belongs to the technical field of biology, and particularly relates to a method for improving in-vitro mature oocyte telomere length and maturation quality and application of the in-vitro mature oocyte telomere length and the maturation quality. And daphnetin. According to the preparation disclosed by the invention, at least one of the Sema3 type protein and the daphnetin is added, so that the telomere length of an oocyte and an in-vitro embryo is effectively maintained, repaired or prolonged, the maturation quality of the in-vitro oocyte and the development potential and development efficiency of the in-vitro embryo are further remarkably improved, and further, the development potential and development efficiency of the in-vitro embryo are further improved. The application provides a more effective new strategy for in-vitro embryo production of livestock, animal genetic improvement, assisted reproduction technology and the like, and the application prospect is wide.

The invention discloses a method for calculating the length of autosomal telomeres of a crowd. The method comprises the following steps: S1, acquiring three-generation sequencing gene long fragment data of different age groups of the crowd; s2, identifying a long fragment containing a telomere sequence; s3, comparing the long fragment data of the third-generation sequencing gene in the step S1 to a reference genome; s4, grouping in chromosome and sequence directions according to a comparison result; s5, identifying and extracting positions of telomere sequences in the same chromosome and direction; sequencing according to the front and back positions of the telomere sequence on the chromosome, and determining a minimum starting position and a maximum ending position; according to the method, the advantage of long reading length of third-generation sequencing is utilized, the whole telomere area can be directly crossed, deviation caused by reading length of second-generation sequencing is avoided, the measurement result is more accurate and reliable, the efficiency is high, and the cost is low.

The invention relates to the field of molecular detection and biological sample treatment, and discloses a saliva collection, pretreatment and cell sorting method for telomere length detection. The method selects saliva as a sample and comprises the following steps: pre-treating the saliva sample; neutrophils in the saliva sample are obtained through magnetic bead sorting, and the obtained neutrophils are used for fluorescent quantitative telomere length detection after being subjected to nucleic acid extraction. According to the method, saliva is selected as a telomere detection sample, no trauma is caused to a human body, and the problems that at present, sample collection is unqualified, transportation is improper, the quality of the saliva sample is unqualified due to long-time placement, follow-up treatment sorting and genome extraction are affected, and correct telomere detection is greatly interfered are effectively solved. Besides, through pretreatment and magnetic bead sorting of a saliva sample, target cells are collected, interference of saliva impurities is reduced, the integrity of the extracted genome DNA is high, the concentration of the target genome is high, subsequent fluorescent quantitative detection is facilitated, and the detection result is more accurate.

The application discloses H3F3A beta protein involved in regulating telomere length and application thereof. The amino acid sequence of the H3F3A beta protein is shown in the sequence table SEQ ID NO:1. The application constructs H3F3A beta specific knockout cell lines and H3F3A beta mutant recovery cell lines, finds that compared with a wild type control group, the telomere length of the H3F3A beta specific knockout cell lines is significantly shortened, and cell proliferation is significantly slowed down, the telomere length of the H3F3A beta mutant gene recovery cell lines is significantly lengthened, and cell proliferation is significantly accelerated, proves that H3F3A beta plays a biological role in maintaining the length of telomeres, and has an effective inhibiting effect on the shortening of the length of telomeres by overexpressing H3F3A beta. Since the length of telomeres can directly affect the aging process of the body, therefore, the H3F3A beta and the mutant related genes or the recombinant expression vector provided by the application have the potential for preparing anti-aging drugs.

According to the human physiological age prediction method based on the telomere length, the aging degree of an individual is quantified by the telomere loss degree on the basis of the telomere length index of white blood cells in crowd blood, and meanwhile, a machine learning model for predicting the physiological age is constructed on the basis of the telomere length and the actual calendar age; the method is used for evaluating the aging condition of the human body relative to the real age. In the prediction process, firstly, the abnormal distribution value of telomere data at each age is identified and filtered, and in the machine learning model construction process, physiological age models are constructed for men and women at different age groups so as to accurately evaluate the health condition and the aging degree of individuals. Therefore, the human physiological age prediction method based on the telomere length has the advantages of being good in prediction effect and high in accuracy.

A method of treating a cancer in a patient includes obtaining a sample from the patient, using a C-circle assay to detect a presence of an alternative lengthening of telomeres (ALT) phenotype in the sample, and administering an effect amount of at least one of PRIMA-1 or APR-246 to the patient.

The application belongs to the technical field of biological medicine, and particularly relates to a composition for improving NAD+ level and a preparation method and application thereof. The composition is prepared by compounding NMN and urolithin A. Through synergistic effect of the two, the problem of limited efficacy of single component is effectively solved. The composition can efficiently improve NAD+ level, prolong telomere length, improve sleep quality under the condition of halving the dosage, improve aging-related indexes from multiple targets and multiple dimensions, and improve product safety and economy, thereby providing a scientific basis for development of related functional products.

The invention discloses a primer pair for evaluating large yellow croaker cell senescence based on relative telomere length and a method thereof, and belongs to the field of molecular genetics. The specific method comprises the following steps: 1) taking DNA of a large yellow croaker cell genome; 2) performing qPCR amplification on the DNA obtained in the step S1 by using a first qPCR amplification system containing a telomere universal primer to obtain a telomere copy number Cttel; (3) carrying out qPCR amplification on the obtained DNA by using a second qPCR amplification system containing the forward primer as shown in SEQ ID No.1 and the reverse primer as shown in SEQ ID No.2, so as to obtain the copy number Ctactb2 of the internal reference single copy gene; and 4) calculating the relative telomere length T / S = 2-[delta] Ct, wherein [delta] Ct = Ctel-Ctactb2. The relative telomere length detection actb2 primer provided by the invention can specifically amplify large yellow croaker DNA, and can realize accurate detection by adopting a trace sample.