33 results about "Karyotype" patented technology

The invention provides a full-automatic indirect immunofluorescence interpretation method and system, a storage medium and a program product, and relates to the technical field of indirect immunofluorescence detection.The method comprises the steps that when identification information of a target sample is obtained, a plurality of sealed antigen slides are placed in slide carrying groove positions of a bearing platform in batches, and the target sample is obtained; driving the bearing platform through the transmission mechanism to sequentially move the plurality of antigen slides to the imaging area, and performing positioning and fixing operation on the antigen slides entering the imaging area by using the positioning and clamping device; sequentially carrying out multi-view scanning on the antigen slides entering the imaging area to obtain multi-view fluorescence images, and splicing the fluorescence images to generate a fluorescence panoramic image; performing feature extraction on the panoramic image, determining a fluorescence karyotype category and a target fluorescence intensity value, and determining an antibody titer according to the category, the intensity value and dilution information; and performing association mapping on the titer, the category and the panoramic image according to the identification information to generate a fluorescence interpretation result.

The invention provides a chromosome image enhancement method and system based on semantic guidance. The method comprises the following steps: S1, preprocessing; s2, outputting a deep-band probability graph, a grey-band probability graph and a shallow-band probability graph through a semantic segmentation network composed of a lightweight encoder and a multi-scale decoder; s3, implementing differential layered enhancement according to a band type: adopting local adaptive histogram equalization for a deep band, adopting central axis constraint bilateral filtering for a gray band, adopting dynamic threshold truncation and gamma correction for a shallow band, and performing weighted fusion for a transition region according to probability; s4, carrying out structure strengthening, wherein the structure strengthening comprises centromere local sharpening, stripe phase alignment, edge sensing super-resolution and overlapping region separation; and S5, performing quality evaluation based on the deep band integrity, the band stripe contrast uniformity, the SSIM and the noise density, and triggering adaptive re-enhancement if necessary. According to the scheme, the contrast ratio and details are remarkably improved while the stripe structure and the position relation are kept, and the method has the advantages of light weight, interpretability and cross-sample robustness and is suitable for being integrated into an automatic karyotype analysis process.

The invention discloses a chromosome image generation method and device for deep learning. Performing adaptive multi-scale image enhancement processing on the chromosome mid-term image to obtain an enhanced chromosome mid-term image; obtaining a chromosome karyotype arrangement diagram based on the enhanced chromosome metaphase image; generating a chromosome set based on the chromosome karyotype arrangement diagram; sequentially merging the chromosomes in the chromosome set into one image as a chromosome foreground image; fusing the chromosome foreground image with a randomly selected background image, and carrying out edge smoothing processing on the fused image to generate a random background chromosome middle-term image; wherein the random background chromosome metaphase image is used for training a target detection model established based on deep learning. According to the method, high-quality and diversified chromosome metaphase images can be generated, and when the method is used for training the deep learning model, the generalization ability and precision of the deep learning model in a target detection task are improved.

The application discloses a construction method and application of an ovary cell line of Quasipaa spinosa. The method comprises the following steps: selecting metamorphosis period larva ovary tissue, disinfecting and obtaining the ovary tissue, and then using collagenase I digestion solution to digest the ovary tissue at 26 DEG C for 1 hour; after the tissue block adheres to the wall, using a low-osmotic-pressure complete culture medium to carry out primary culture at 25-27 DEG C, wherein the culture medium is 60% diluted DMEM / F12 culture medium containing 10%-15% fetal bovine serum; when the cell confluence degree is higher than 70%, using trypsin-EDTA to digest the cells for 1-2 minutes to subculture, and the subculture culture medium contains 30% cell conditional culture medium; and finally, the cells are cryopreserved through programmed cooling. The ovary epithelial cell line of Quasipaa spinosa is successfully established for the first time, the survival rate of the cells after recovery is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and in-vitro conservation of germplasm resources.

The invention discloses a chromosome karyotype analysis method and system, and relates to the technical field of image recognition, and the method comprises the following steps: S1, obtaining to-be-analyzed chromosome image data, and marking the to-be-analyzed chromosome image data to obtain a to-be-analyzed image data set; s2, introducing an improved module to perform network reconstruction on the YOLOv11 network model to obtain a chromosome detection model; s3, training a chromosome detection model through the to-be-analyzed image data set, and performing performance evaluation to obtain a target chromosome detection model; and S4, analyzing the real-time chromosome image data through the target chromosome detection model, and outputting an analysis result. According to the scheme, the detection capability of the model on chromosomes in small-target, abnormal and complex scenes is enhanced by utilizing an improved module, and the problems of low accuracy and poor robustness in the prior art are solved, so that efficient and accurate chromosome karyotype analysis is realized, and clinical and occupational health examination requirements are met.

The application belongs to the technical field of biology, and discloses a use of antisense nucleotide of piR-bmo-796514 nucleotide for preparing a medicine for inhibiting Bombyx mori nuclear polyhedrosis virus, the antisense nucleotide of the piR-bmo-796514 nucleotide is mainly used for inhibiting replication of the Bombyx mori nuclear polyhedrosis virus in ovary cells of the Bombyx mori, and a sequence of the piRNA nucleotide is shown as SEQ ID NO:1; in addition, the application also discloses the antisense nucleotide of the piR-bmo-796514 nucleotide, and an antisense nucleotide sequence is shown as SEQ ID NO:2.

The invention discloses an SSR molecular marker for screening nuclear cracking resistance characters of peaches and application of the SSR molecular marker, and relates to the technical field of genetic breeding of fruit trees, the SSR molecular marker is named as PpCPK12-SSR, and the nucleotide sequence of the SSR molecular marker is shown as SEQ ID NO.1. The invention also provides a specific primer pair for amplifying the marker, the nucleotide sequence of an upstream primer of the specific primer pair is shown as SEQ ID NO.2, and the nucleotide sequence of a downstream primer of the specific primer pair is shown as SEQ ID NO.2. The nucleotide sequence of the downstream primer is as shown in SEQ ID NO.3. Through PCR (polymerase chain reaction) amplification and electrophoresis detection, according to the size of an amplified fragment, the anti-nuclear-cleavage genotype of the peach can be quickly and accurately identified in the seedling stage of the peach: an individual carrying a 115bp fragment is an anti-nuclear-cleavage type; the defects that traditional field phenotype identification is long in period, low in efficiency and affected by the environment are overcome, an efficient molecular marker auxiliary selection tool is provided for peach nuclear cracking resistance breeding, and the breeding process can be remarkably accelerated.

The invention provides a novel human distal bile duct cancer cell line with TP53 missense mutation and application, the novel human distal bile duct cancer cell line CBC3T-3 is established, the cell line is preserved in the China Center for Type Culture Collection (the preservation number is CCTCC NO: C202555), the uniqueness and stability of the cell line are proved through STR typing and karyotype analysis, and the TP53 missense mutation novel human distal bile duct cancer cell line has the advantages that the TP53 missense mutation novel human distal bile duct cancer cell line CBC3T-3 can be used for preparing the TP53 missense mutation novel human distal bile duct cancer cell line CBC3T-3; and a plurality of driver gene mutations including TP53 missense mutation are carried. The CBC3T-3 has strong proliferation, invasion and migration capabilities, has high tumor formation rate in immunodeficient mice, is resistant to cis-platinum and sensitive to paclitaxel and gemcitabine, and provides an experimental basis for selection of clinical chemotherapy regimens. According to the model, the TP53 missense mutation type distal bile duct cancer in-vitro model is successfully established, and a key experimental platform is provided for deeply researching the drug resistance mechanism of the TP53 missense mutation type distal bile duct cancer and developing an individualized treatment strategy aiming at the subtype of the TP53 missense mutation type distal bile duct cancer.

The invention provides an improved euchiloglanis kishinouyei chromosome preparation method, which comprises the following steps: gradually raising the temperature and injecting PHA twice: in the euchiloglanis kishinouyei temporary culture process, raising the water temperature from 18 DEG C to 20 DEG C within 12 hours, injecting a first needle of PHA, raising the temperature to 22.5-23 DEG C after 12 hours, injecting a second needle of PHA, and keeping the water temperature at 22.5-23 DEG C unchanged; after the PHA is injected for the second time for 3 hours, colchicine is injected; sampling: washing the head and kidney in normal saline, and collecting cells; 0.5% of KCl is used for hypotonic treatment; fixing with a Carnoy's fixing solution, and centrifuging; dropping by a cold dropping method and naturally drying; and carrying out Giemsa dyeing and flaking. According to the method, the euchiloglanis kishinouyei somatic cell proliferation is promoted through heating and twice PHA injection, so that the metaphase division phase is increased, the form is clear and dispersed, and the karyotype quality and the preparation success rate of the chromosome are remarkably improved.

The present application relates to the field of aquatic organism breeding technology, and particularly relates to a method for preparing rainbow trout tetraploid fry. The method provided by the present application comprises the following steps: obtaining fertilized eggs by dry fertilization of naturally matured rainbow trout parents, inducing the rainbow trout fertilized eggs to obtain tetraploid rainbow trout after 6.2-6.4 hours, and the duration of the induction is 10-18 minutes; after the tetraploid rainbow trout fry is obtained by induction, the chromosome ploidy of the fish is determined by using techniques such as chromosome karyotype analysis and flow cytometry DNA content determination, and the tetraploid rainbow trout fry is identified and screened for cultivation. The present application adopts a drug induction method, and controls the starting time and duration of the induction to inhibit the first mitosis time of the fertilized eggs, thereby improving the induction rate and survival rate of the tetraploid rainbow trout, and the induction rate of the tetraploid rainbow trout can reach 25-30%, and the survival rate of the fry can reach more than 90%.

The invention relates to the technical field of cell separation and purification and cell biological detection, in particular to a method for separating and purifying fetal NRBC from peripheral blood of a pregnant woman. The invention provides a method for separating and purifying fetal NRBC from peripheral blood of a pregnant woman, which is characterized in that a LukSF and LukAB bi-component perforation toxin is designed and utilized to specifically recognize and split a CXCR1 / 2 chemotactic factor receptor and a CD11b / CD18 integrin receptor on the surface of maternal leukocyte, and the fetal NRBC is completely reserved due to lack of corresponding receptors, so that the fetal NRBC can be separated and purified. Therefore, leukocyte pollution in nucleated red blood cells of a peripheral blood fetus of a pregnant woman is efficiently removed. According to the method, the purity of the extracted fetal NRBC can be improved, the false positive of gene detection is greatly reduced, the activity and integrity of the NRBC are reserved, and the accurate diagnosis requirements of chromosome karyotype analysis, monogenic disease detection and the like are met; the method is simple to operate and low in cost, can be directly adapted to existing clinical laboratory equipment, and has large-scale popularization value.

The invention discloses a construction method and application of an ovarian cell line of hapalogenys nitens. The method comprises the following steps: selecting a metamorphosis stage larva ovarian tissue, disinfecting and obtaining, and digesting for 1 hour at 26 DEG C by using collagenase I digestive juice; after the tissue block adheres to the wall, a low-osmotic-pressure complete medium is used for primary culture at the temperature of 25-27 DEG C, and the medium is a 60% diluted DMEM / F12 medium containing 10%-15% of fetal calf serum; after the cell confluence degree exceeds 70%, trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) is used for digesting for 1-2 minutes for passage, and a passage culture medium contains 30% of a cell condition culture medium; and finally performing programmed cooling and cryopreservation. According to the method, the ovarian epithelial cell line of the hapalogenys nitens is successfully established for the first time, the cell recovery survival rate is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and germplasm resource in-vitro preservation.

The invention discloses a chromosome counting method and device based on image processing and electronic equipment. The method comprises the steps that computer equipment acquires a chromosome karyotype graph; projecting each row of pixel points of the chromosome karyotype graph to obtain a first projection vector; determining a plurality of foreground regions of the chromosome karyotype graph according to the first projection vector; selecting a plurality of first foreground areas from the plurality of foreground areas, and extracting target image areas corresponding to the plurality of first foreground areas; projecting each column of pixel points of the target image area to obtain a second projection vector; determining one or more chromosome regions contained in the target image region according to the second projection vector; selecting a target chromosome region comprising the chromosomes of the target type from one or more chromosome regions contained in the target image region; and obtaining the chromosome number corresponding to the chromosome of the target type. According to the invention, the counting accuracy and efficiency of the chromosome karyotype graph can be improved.

The application relates to a chromosome image segmentation and recognition method and system based on deep learning, and relates to the technical field of medical image processing.The method comprises the following steps: performing target detection processing on original chromosome image data to output chromosome image data with labeled chromosome clusters; using a dynamic convolution kernel and performing multi-level convolution and down-sampling processing on the chromosome image data to obtain intermediate layer down-sampling feature data and final layer down-sampling feature data; combining a parameter-free attention mechanism, performing deep separable convolution processing and pixel recombination processing on the final layer down-sampling feature data to obtain deep layer feature optimization data; performing inverse convolution processing and jump connection splicing processing on the deep layer feature optimization data and the intermediate layer down-sampling feature data to output chromosome segmentation image data; and performing classification and arrangement processing on the chromosome segmentation image data to obtain a chromosome karyotype analysis result.The chromosome image segmentation and recognition method and system can achieve good segmentation and recognition effects on chromosome images.

The invention discloses a preparation method of chromosomes of tiny insects trichogramma dendrolimus, and belongs to the technical field of chromosome preparation. Comprising the following steps: (1) preparing a pre-pupa material: selecting corcyra cephalonica eggs parasitized by trichogramma dendrolimus for 4-8 days, and cutting out a trichogramma dendrolimus pre-pupa; (2) pretreatment: pretreating the pre-pupa material in a colchicine solution; (3) low-permeability treatment: carrying out low-permeability treatment on the pretreated material in a low-permeability solution; and (4) fixing, uncovering, dyeing and sealing the material subjected to the low-permeability treatment. The invention provides a simpler trichogramma dendrolimus chromosome slide preparation method, chromosome slides with clear forms can be prepared in batches, the dispersion degree of chromosomes is large, the length, centromere position, arm ratio and other characteristics of the chromosomes can be observed more clearly, the chromosome characteristics are determined through karyotype analysis, and the chromosome slide preparation method is suitable for large-scale production. And a foundation is laid for cell level research and genomics deep research of trichogramma dendrolimus.

The application provides a miscarriage risk assessment method and system of whole-exome load and serum markers, comprising: independently scoring each variation in whole-exome sequencing data to obtain a comprehensive pathogenic score; constructing a gene pathogenic load matrix; mapping the gene pathogenic load to a biological pathway to obtain a pathway comprehensive score and construct a model; solving the model by jointly optimizing the regularization strength parameters of the two-layer ridge penalty terms, outputting significant pathway features; standardizing and preprocessing serum detection data; jointly modeling the output significant pathway features and the output standardized serum data under the same logistic regression probability framework, outputting a miscarriage-related risk probability value, and performing probability stratification. The application also outputs a double-track coordination coefficient to quantify the directional consistency of genetic signals and serum signals. It is significantly superior to a single omics solution and provides a high-precision individualized risk assessment tool for recurrent miscarriage with normal karyotype.

The invention discloses application of an METTL14 inhibitor in preparation of a medicine for treating malignant tumors carrying DNA outside chromosomes, and belongs to the technical field of medicines. A human colon cancer cell HT29-4 containing ecDNA and an ovarian cancer cell UACC-1598-4 are selected as research objects, an METTL14 stable knock-down and overexpression cell model is constructed through a lentivirus-mediated RNA interference technology, and the knock-down and overexpression efficiency is verified through Western Blot. Kyotype analysis shows that the quantity of ecDNA is reduced by knocking down the METTL14, and the quantity of the ecDNA is increased by overexpressing the METTL14. The copy number of DHFR and EIF5A2 genes carried by ecDNA is detected through qPCR, and the result shows that the copy number is decreased after METTL14 is knocked down, and the copy number of overexpression groups is increased. EdU proliferation experiments and cell scratch experiments show that knock-down of METTL14 inhibits proliferation and migration ability of tumor cells containing ecDNA, and overexpression promotes the phenotypes. The invention provides an effective treatment means for the intractable tumor driven by the ecDNA.

The application discloses a chromosome image generation method and device for deep learning. Adaptive multi-scale image enhancement processing is performed on a chromosome metaphase image to obtain an enhanced chromosome metaphase image; a chromosome karyotype arrangement map is obtained based on the enhanced chromosome metaphase image; a chromosome set is generated based on the chromosome karyotype arrangement map; chromosomes in the chromosome set are sequentially merged into one image as a chromosome foreground image; the chromosome foreground image is fused with a randomly selected background image, and edge smoothing processing is performed on the fused image to generate a random background chromosome metaphase image; wherein the random background chromosome metaphase image is used for training a target detection model established based on deep learning. The method can generate high-quality and diversified chromosome metaphase images, and thus is used for training a deep learning model, and improves the generalization ability and precision of the deep learning model in a target detection task.

The invention discloses establishment and application of a humpback perch frontal bone tissue osteoblast line, and relates to the technical field of biology. The cell line is a humpback perch frontal bone tissue osteoblast line CAFBOB, the preservation number is CCTCC NO: C2025129, and the cell line is derived from a humpback perch juvenile fish frontal bone tissue and is established after tissue separation, primary culture by a dry pasting method, subculture and cryopreservation resuscitation. The biological characteristics are stable, 1-40 generations are mainly long fusiform, the chromosome karyotype is 2n = 48, strong alkaline phosphatase activity is achieved, the transfection efficiency is about 85%, and the activity is good after cryopreservation and resuscitation. The establishment method is high in repeatability, the obtained cell line can be used as an in-vitro research model and applied to morphological specialization mechanism analysis, osteogenesis-related gene function verification, classification dispute solution and seeding process research of the humpback perch, the blank of the bone tissue cell line of the humpback perch is filled, and the bone tissue cell line has important scientific research value and application prospect.

The invention discloses a macaque skin squamous carcinoma cell line MCSCC14397 and application of the macaque skin squamous carcinoma cell line MCSCC14397. The macaque skin squamous carcinoma cell line is named as macaque skin squamous carcinoma cell line MCSCC14397, and the preservation number of the macaque skin squamous carcinoma cell line is CCTCC (China Center For Type Culture Collection) NO: C2025156. The cell line disclosed by the invention has similar protein expression with primary tumor tissues, has chromosome abnormal karyotype, in-vitro tumor formation capability and stable proliferation characteristics, and simultaneously shows remarkable genome instability (such as high-frequency Cgt, T mutation and structural variation) and inflammation signal activation. The MYO10 gene expression in the cell line is up-regulated and drives DNA damage reaction and inflammatory factor expression, and unique advantages are provided for application of the cell line in skin squamous cell carcinoma mechanism research, drug screening and drug efficacy evaluation. The cell line disclosed by the invention can be used as an effective cell model, can be used for establishing a xenotransplantation animal model, and can provide a research basis for deeply researching the occurrence, development, metastasis mechanism and drug resistance mechanism of human skin squamous cell carcinoma as well as innovative drug development and screening.

An improved baculovirus expression system for enhanced heterologous protein expression in hosts selected from Bombyx mori larvae, pupae, primary cells, or established insect cell lines is disclosed. The expression system is derived from a wild-type strain of Bombyx mori nucleopolyhedrovirus (BmNPV) by deletion or inactivation of non-essential genes, of the BmNPV genome. The system includes novel transfer vectors designed for targeted gene modification and incorporation of a bacterial origin of replication and an insertion site for a gene of interest (GOI). This engineered bacmid maintains infectivity while offering enhanced genetic stability and significantly improved heterologous protein expression in insect cells, larvae, or pupae. Methods for constructing this bacmid via homologous recombination and using it for high-level protein production are also described.

The invention discloses establishment and application of an epinephelus lanceolatus frontal bone tissue osteoblast line, and relates to the technical field of biology. The cell line is an epinephelus lanceolatus frontal bone tissue osteoblast line ELFBOB, the preservation number is CCTCC NO: C2025132, the cell line is derived from epinephelus lanceolatus fry frontal bone tissue, and the cell line is established after tissue separation, primary culture with a dry pasting method, subculture and cryopreservation resuscitation. The biological characteristics are stable, 1-40 generations are mainly long fusiform, the chromosome karyotype is 2n = 48, strong alkaline phosphatase activity is achieved, the transfection efficiency is about 75%, and the activity is good after cryopreservation and resuscitation. The establishment method is high in repeatability, the obtained cell line can serve as an in-vitro research model and is applied to epinephelus lanceolatus skull malformation cause analysis, osteogenesis-related gene function verification, improved variety breeding and healthy culture strategy optimization, the blank of the epinephelus lanceolatus bone tissue cell line is filled, and the method has important scientific research value and industrial application prospects.

The application provides a fluorescent image intelligent recognition method, system, device and medium, and relates to the technical field of image recognition. The method comprises the following steps: acquiring a plurality of original fluorescent images corresponding to a plurality of dilutions of a sample; extracting texture morphological features, fluorescent bright spot spatial distribution features and average fluorescent intensity of a cell nucleus region in a target original fluorescent image, the target original fluorescent image being any one of the plurality of original fluorescent images; fusing the texture morphological features and the fluorescent bright spot spatial distribution features to obtain a mixed feature vector; judging the mixed feature vector through a preset karyotype classification model to obtain a karyotype category of the target original fluorescent image; and calculating an antibody titer value according to the plurality of dilutions, the karyotype category corresponding to each dilution and the average fluorescent intensity corresponding to each dilution. The technical solution provided by the application improves the efficiency, objectivity and accuracy of detection.

The invention belongs to the technical field of cell culture, and discloses a bone marrow cell culture medium and a preparation method thereof, and a method for in-vitro culture of bone marrow cells, the bone marrow cell culture medium is composed of a basic culture medium, streptomycin, gentamicin, a platelet lysis buffer, and glutamate enzyme; the final concentration of streptomycin is 50-100 [mu] g / mL, the final concentration of gentamicin is 20-50 [mu] g / mL, the final concentration of platelet lysate is 5-15% (v / v), and the final concentration of glutamate enzyme is 0.1-1 mmol / L. The bone marrow cell culture medium can efficiently support in-vitro proliferation of bone marrow cells, through specific combination and appropriate concentration control of the culture medium, the medium-term cell acquisition amount can be remarkably increased, and medium-term cell chromosomes are complete in morphology and good in dispersity; the standard requirements of clinical bone marrow cell chromosome karyotype analysis, blood disease diagnosis and cell genetics experiments in the scientific research field can be met.

The chromosome classification processing method and system based on self-supervised contrast learning relates to the technical field of image processing; the chromosome classification processing method and system comprises obtaining a preliminary chromosome classification result; according to a self-defined homologous chromosome loss function, self-supervised contrast learning is performed on all first chromosome images and second chromosome images to obtain chromosome similarity analysis results of all first chromosome images and second chromosome images; the homologous chromosome loss function introduces a homologous similarity constraint term, and the chromosome similarity analysis results include the chromosome similarity of the first chromosome image and each second chromosome image; according to the preliminary chromosome classification result, the chromosome similarity analysis result and a priority matching rule, a final chromosome classification result is output, so as to solve the problem that the feature similarity of homologous chromosomes is not captured by the existing pre-training classification model, resulting in classification errors and affecting the accuracy of chromosome karyotype analysis.

An improved baculovirus expression system for enhanced heterologous protein expression in hosts selected from Bombyx mori larvae, pupae, primary cells, or established insect cell lines is disclosed. The expression system is derived from a wild-type strain of Bombyx mori nucleopolyhedrovirus (BmNPV) by deletion or inactivation of non-essential genes, of the BmNPV genome. The system includes novel transfer vectors designed for targeted gene modification and incorporation of a bacterial origin of replication and an insertion site for a gene of interest (GOI). This engineered bacmid maintains infectivity while offering enhanced genetic stability and significantly improved heterologous protein expression in insect cells, larvae, or pupae. Methods for constructing this bacmid via homologous recombination and using it for high-level protein production are also described.

The application provides a Chinese lung large cell neuroendocrine carcinoma cell line with high lymph node metastasis potential and an application, and the preservation number of the cell line is CCTCC NO: C2024277. The cell line of the application is a lung large cell neuroendocrine carcinoma cell line which is rare in the histopathological type of lung cancer, has no homology with any human lung cancer cell line of other histopathological types, and has an abnormal karyotype. The cell line of the application can be continuously subcultured in vitro while keeping the biological characteristics of lung large cell neuroendocrine carcinoma tumor cells unchanged; has an in-vitro unlimited proliferation capacity, a good invasion and migration capacity, can induce subcutaneous tumor formation in mice, has a strong popliteal lymph node metastasis capacity in mice, and is suitable for being used as an in-vitro cell platform and an animal model cell line for researching the molecular mechanism of the occurrence, development and metastasis of the rare lung large cell neuroendocrine carcinoma and related diagnosis, treatment and drug research.

The application provides a chromosome image analysis method and system based on progressive segmentation and focused classification, and comprises the following steps: a progressive segmentation step: performing segmentation on a metaphase chromosome image by using progressive segmentation to obtain a single chromosome image set; a focused classification step: identifying the categories of all single chromosomes in the single chromosome image set by using a focused classification algorithm, and finally generating a karyotype analysis graph. The application combines a traditional chromosome processing method and deep learning to form a progressive segmentation method, which gradually and effectively separates the adherent chromosome clusters, has high segmentation precision, and does not involve manual participation.

Provided are a fusion protein comprising a mutated kinetochore protein and dCas9. The fusion protein is used in conjunction with guide RNAs target the fusion protein to a location of kinetochore assembly on a centromere such that the fusion protein interferes with chromosome segregation. Use of the fusion protein and the guide RNAs in cells results the cells acquiring an aneuploidy karyotype. Expression vectors that encode the fusion proteins and / or the guide RNAs and their uses in the method of producing an aneuploidy karyotype are also provided.

This invention relates to the fields of tumor biology and cell engineering, specifically disclosing the construction and application of a human prostate cancer cell line. The human prostate cancer cell line is derived from primary prostate cancer tissue, expresses prostate-specific antigen and α-methylacyl-CoA racemic enzyme, and has a 46,XY karyotype. The construction method employs an innovative strategy of co-culturing fully digested cell suspensions with incompletely digested tissue blocks, significantly improving the success rate of establishing the cell line from the primary lesion. This cell line can stably proliferate in conventional culture media supplemented with testosterone and has been successfully applied to subcutaneous tumorigenesis models in immunodeficient mice and in vivo migration models in zebrafish. The primary lesion-derived prostate cancer cell line provided by this invention offers a highly relevant, stable, and reliable new experimental tool for studying the biological characteristics of primary prostate cancer, drug screening, and personalized treatment.