73 results about "Karyotype" patented technology

The invention relates to the technical field of molecular biology, and particularly provides an InDel molecular marker related to the size of a lychee kernel and application of the InDel molecular marker. The InDel molecular marker is located at a site 25610680 of a fifth chromosome of a reference genome GeneBank: GCA019925255.1 of the litchi, and the InDel molecular marker is used for detecting the DNA of the litchi. The genotype of the marker in a pyrokaryotic litchi material is homozygous' GAAAAA / GAAAAA ', and the genotype of the marker in a macrokaryotic litchi material is heterozygous' G---- / GAAAAA 'or homozygous' G---- / G----'. The invention further provides a primer group for detecting the molecular marker, an effective tool is provided for molecular marker-assisted breeding of the size of the lychee kernel, and the method is expected to assist in shortening the breeding period, improving the breeding efficiency and promoting the improvement work of the lychee variety.

The invention provides a full-automatic indirect immunofluorescence interpretation method and system, a storage medium and a program product, and relates to the technical field of indirect immunofluorescence detection.The method comprises the steps that when identification information of a target sample is obtained, a plurality of sealed antigen slides are placed in slide carrying groove positions of a bearing platform in batches, and the target sample is obtained; driving the bearing platform through the transmission mechanism to sequentially move the plurality of antigen slides to the imaging area, and performing positioning and fixing operation on the antigen slides entering the imaging area by using the positioning and clamping device; sequentially carrying out multi-view scanning on the antigen slides entering the imaging area to obtain multi-view fluorescence images, and splicing the fluorescence images to generate a fluorescence panoramic image; performing feature extraction on the panoramic image, determining a fluorescence karyotype category and a target fluorescence intensity value, and determining an antibody titer according to the category, the intensity value and dilution information; and performing association mapping on the titer, the category and the panoramic image according to the identification information to generate a fluorescence interpretation result.

The invention provides a chromosome image enhancement method and system based on semantic guidance. The method comprises the following steps: S1, preprocessing; s2, outputting a deep-band probability graph, a grey-band probability graph and a shallow-band probability graph through a semantic segmentation network composed of a lightweight encoder and a multi-scale decoder; s3, implementing differential layered enhancement according to a band type: adopting local adaptive histogram equalization for a deep band, adopting central axis constraint bilateral filtering for a gray band, adopting dynamic threshold truncation and gamma correction for a shallow band, and performing weighted fusion for a transition region according to probability; s4, carrying out structure strengthening, wherein the structure strengthening comprises centromere local sharpening, stripe phase alignment, edge sensing super-resolution and overlapping region separation; and S5, performing quality evaluation based on the deep band integrity, the band stripe contrast uniformity, the SSIM and the noise density, and triggering adaptive re-enhancement if necessary. According to the scheme, the contrast ratio and details are remarkably improved while the stripe structure and the position relation are kept, and the method has the advantages of light weight, interpretability and cross-sample robustness and is suitable for being integrated into an automatic karyotype analysis process.

The invention discloses a chromosome image generation method and device for deep learning. Performing adaptive multi-scale image enhancement processing on the chromosome mid-term image to obtain an enhanced chromosome mid-term image; obtaining a chromosome karyotype arrangement diagram based on the enhanced chromosome metaphase image; generating a chromosome set based on the chromosome karyotype arrangement diagram; sequentially merging the chromosomes in the chromosome set into one image as a chromosome foreground image; fusing the chromosome foreground image with a randomly selected background image, and carrying out edge smoothing processing on the fused image to generate a random background chromosome middle-term image; wherein the random background chromosome metaphase image is used for training a target detection model established based on deep learning. According to the method, high-quality and diversified chromosome metaphase images can be generated, and when the method is used for training the deep learning model, the generalization ability and precision of the deep learning model in a target detection task are improved.

A human chronic myeloid leukemia cell line and the use thereof. The human chronic myeloid leukemia cell line is the first cell line internationally established from chronic-phase leukemia cells of chronic myeloid leukemia, and was named human chronic myeloid leukemia cell YYXY-M6, which was deposited at the China Center for Type Culture Collection (Wuhan University, Wuhan, China) on July 24, 2023, under the deposit number of CCTCC NO: C2023219. The leukemia cell line exhibits primitive cell morphology and has three karyotypes, i.e. t(6:11)(q25:q23), del(11)(q23) and normal karyotype (46, XX); is BCR-ABL gene-negative; has good in-vitro proliferation ability; can be used as cellular material for the study of the mechanism of occurrence and development of the chronic phase of chronic myeloid leukemia, from the chronic phase thereof to the blastic phase thereof, and of BCR-ABL gene-negative chronic myeloid leukemia, and for the in-vitro study of individualized treatment; and can also be used for both in-vitro and in-vivo studies of drug screening and evaluation for the chronic phase of chronic myeloid leukemia, from the chronic phase thereof to the blastic phase thereof, and BCR-ABL gene-negative chronic myeloid leukemia, providing guidance for clinical medication.

The application discloses a construction method and application of an ovary cell line of Quasipaa spinosa. The method comprises the following steps: selecting metamorphosis period larva ovary tissue, disinfecting and obtaining the ovary tissue, and then using collagenase I digestion solution to digest the ovary tissue at 26 DEG C for 1 hour; after the tissue block adheres to the wall, using a low-osmotic-pressure complete culture medium to carry out primary culture at 25-27 DEG C, wherein the culture medium is 60% diluted DMEM / F12 culture medium containing 10%-15% fetal bovine serum; when the cell confluence degree is higher than 70%, using trypsin-EDTA to digest the cells for 1-2 minutes to subculture, and the subculture culture medium contains 30% cell conditional culture medium; and finally, the cells are cryopreserved through programmed cooling. The ovary epithelial cell line of Quasipaa spinosa is successfully established for the first time, the survival rate of the cells after recovery is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and in-vitro conservation of germplasm resources.

The invention discloses a chromosome karyotype analysis method and system, and relates to the technical field of image recognition, and the method comprises the following steps: S1, obtaining to-be-analyzed chromosome image data, and marking the to-be-analyzed chromosome image data to obtain a to-be-analyzed image data set; s2, introducing an improved module to perform network reconstruction on the YOLOv11 network model to obtain a chromosome detection model; s3, training a chromosome detection model through the to-be-analyzed image data set, and performing performance evaluation to obtain a target chromosome detection model; and S4, analyzing the real-time chromosome image data through the target chromosome detection model, and outputting an analysis result. According to the scheme, the detection capability of the model on chromosomes in small-target, abnormal and complex scenes is enhanced by utilizing an improved module, and the problems of low accuracy and poor robustness in the prior art are solved, so that efficient and accurate chromosome karyotype analysis is realized, and clinical and occupational health examination requirements are met.

A chromosome image segmentation identification method and system based on deep learning relates to the technical field of medical image processing, and comprises the following steps: performing target detection processing on original chromosome image data, and outputting chromosome image data calibrated with chromosome clusters; performing multi-level convolution and down-sampling processing on the chromosome image data by using a dynamic convolution kernel to obtain middle-layer down-sampling feature data and final-layer down-sampling feature data; in combination with a parameter-free attention mechanism, performing depth separable convolution processing and pixel recombination processing on the sampling feature data under the final layer to obtain deep feature optimization data; performing deconvolution processing and jump connection splicing processing on the deep feature optimization data and the intermediate layer down-sampling feature data, and outputting chromosome segmentation image data; classifying and arranging the chromosome segmentation image data to obtain a chromosome karyotype analysis result; the chromosome image segmentation and identification method and system have good segmentation and identification effects on the chromosome image.

The application belongs to the technical field of biology, and discloses a use of antisense nucleotide of piR-bmo-796514 nucleotide for preparing a medicine for inhibiting Bombyx mori nuclear polyhedrosis virus, the antisense nucleotide of the piR-bmo-796514 nucleotide is mainly used for inhibiting replication of the Bombyx mori nuclear polyhedrosis virus in ovary cells of the Bombyx mori, and a sequence of the piRNA nucleotide is shown as SEQ ID NO:1; in addition, the application also discloses the antisense nucleotide of the piR-bmo-796514 nucleotide, and an antisense nucleotide sequence is shown as SEQ ID NO:2.

The present invention belongs to the technical fields of molecular biology and molecular breeding, and particularly relates to SNP molecular markers related to Bombyx mori nucleopolyhedrovirus (BmNPV) resistance and their applications. The SNP molecular markers include the combination of the Chr3-746 marker and the Chr27-5071 marker; the Chr3-746 marker is located at the 14951064th position of the 3rd chromosome sequence of the Bombyx mori p50T genome, and the base polymorphism is G / A; the Chr27-5071 marker is located at the 9462596th position of the 27th chromosome sequence of the Bombyx mori p50T genome, and the base polymorphism is C / G. The SNP molecular markers of the present invention can effectively distinguish BmNPV-resistant and -susceptible Bombyx mori, reduce the false positive rate of detection, improve the accuracy rate, and can be screened quickly and in a high-throughput manner, solve the technical problems of traditional Bombyx mori breeding, and accelerate the breeding of BmNPV-resistant Bombyx mori varieties.

The invention discloses an SSR molecular marker for screening nuclear cracking resistance characters of peaches and application of the SSR molecular marker, and relates to the technical field of genetic breeding of fruit trees, the SSR molecular marker is named as PpCPK12-SSR, and the nucleotide sequence of the SSR molecular marker is shown as SEQ ID NO.1. The invention also provides a specific primer pair for amplifying the marker, the nucleotide sequence of an upstream primer of the specific primer pair is shown as SEQ ID NO.2, and the nucleotide sequence of a downstream primer of the specific primer pair is shown as SEQ ID NO.2. The nucleotide sequence of the downstream primer is as shown in SEQ ID NO.3. Through PCR (polymerase chain reaction) amplification and electrophoresis detection, according to the size of an amplified fragment, the anti-nuclear-cleavage genotype of the peach can be quickly and accurately identified in the seedling stage of the peach: an individual carrying a 115bp fragment is an anti-nuclear-cleavage type; the defects that traditional field phenotype identification is long in period, low in efficiency and affected by the environment are overcome, an efficient molecular marker auxiliary selection tool is provided for peach nuclear cracking resistance breeding, and the breeding process can be remarkably accelerated.

The invention discloses an oligonucleotide-coated fluorescence in-situ hybridization probe for specifically recognizing a tartary buckwheat Chr1-4 chromosome and application, and belongs to the field of cytological markers. The method comprises the following steps: screening a single-copy oligonucleotide sequence of Chr1-4 from a tartary buckwheat whole genome by using Chorus2 software; filtering and removing repetitive sequences to obtain a Chr1-4 chromosome probe sequence library; screening a specific single copy oligonucleotide sequence of the Chr1-4 chromosome; and adding specific primers at the two ends of each single copy oligonucleotide sequence of Chr1-4, and combining the probes into a buckwheat Chr1-4 Oligo-Painting probe mixing pool. By utilizing the constructed Oligo-Painting probe mixing pool and a fluorescence in-situ hybridization technology, accurate identification and karyotype analysis of the buckwheat chromosomes can be realized, the number, morphology, heritable variation and other structural characteristics of the chromosomes can be known, and an important technical support is provided for cytological analysis of buckwheat crossbreeding.

The invention provides a novel human distal bile duct cancer cell line with TP53 missense mutation and application, the novel human distal bile duct cancer cell line CBC3T-3 is established, the cell line is preserved in the China Center for Type Culture Collection (the preservation number is CCTCC NO: C202555), the uniqueness and stability of the cell line are proved through STR typing and karyotype analysis, and the TP53 missense mutation novel human distal bile duct cancer cell line has the advantages that the TP53 missense mutation novel human distal bile duct cancer cell line CBC3T-3 can be used for preparing the TP53 missense mutation novel human distal bile duct cancer cell line CBC3T-3; and a plurality of driver gene mutations including TP53 missense mutation are carried. The CBC3T-3 has strong proliferation, invasion and migration capabilities, has high tumor formation rate in immunodeficient mice, is resistant to cis-platinum and sensitive to paclitaxel and gemcitabine, and provides an experimental basis for selection of clinical chemotherapy regimens. According to the model, the TP53 missense mutation type distal bile duct cancer in-vitro model is successfully established, and a key experimental platform is provided for deeply researching the drug resistance mechanism of the TP53 missense mutation type distal bile duct cancer and developing an individualized treatment strategy aiming at the subtype of the TP53 missense mutation type distal bile duct cancer.

The invention discloses a quality control product for noninvasive prenatal detection of fetal chromosome trisomy syndrome and a preparation method thereof, the quality control product comprises a positive quality control product and a negative quality control product, the positive quality control product is obtained by adding nucleic acid fragments of trisomy syndrome genome DNA into background plasma; the negative quality control product is obtained by adding nucleic acid fragments of normal karyotype genome DNA into background plasma; the length of the nucleic acid fragment is 130 to 200 bp; the background plasma is peripheral blood plasma of normal karyotype and pregnant fetus women. According to the invention, peripheral blood plasma of a pregnant woman with normal karyotype and pregnant fetus is used as background plasma, a DNA extraction correction coefficient is introduced, and nucleic acid fragments of trisomatic negative and positive genomes 130-200bp with sex chromosome XY are added to prepare the quality control product, so that the quality control product is more accurate and reliable, and is more suitable for pregnant woman plasma; and whether each index of the noninvasive antenatal detection kit meets the technical requirements of a product or not can be reflected more completely and truly.

The invention provides a gene with an antiviral effect and application thereof, the nucleotide sequence of an open reading frame of the gene is SEQ ID NO: 2, and the amino acid sequence of a coding protein of the gene is SEQ ID NO: 1. The invention also provides a method for increasing the sensitivity of the beet armyworm to the beet armyworm nuclear polyhedrosis virus, which is used for reducing the expression quantity of the gene in the beet armyworm. According to the invention, a gene with an antiviral effect is screened from an important agricultural pest beet armyworm. The sequence structure and tissue expression mode of the gene are analyzed, incremental expression and RNA interference technologies are utilized to prove that the gene has an anti-SeMNPV effect, a new target is provided for better utilizing baculovirus to prevent and control beet armyworms in production, and the gene has a good application prospect.

The invention provides an improved euchiloglanis kishinouyei chromosome preparation method, which comprises the following steps: gradually raising the temperature and injecting PHA twice: in the euchiloglanis kishinouyei temporary culture process, raising the water temperature from 18 DEG C to 20 DEG C within 12 hours, injecting a first needle of PHA, raising the temperature to 22.5-23 DEG C after 12 hours, injecting a second needle of PHA, and keeping the water temperature at 22.5-23 DEG C unchanged; after the PHA is injected for the second time for 3 hours, colchicine is injected; sampling: washing the head and kidney in normal saline, and collecting cells; 0.5% of KCl is used for hypotonic treatment; fixing with a Carnoy's fixing solution, and centrifuging; dropping by a cold dropping method and naturally drying; and carrying out Giemsa dyeing and flaking. According to the method, the euchiloglanis kishinouyei somatic cell proliferation is promoted through heating and twice PHA injection, so that the metaphase division phase is increased, the form is clear and dispersed, and the karyotype quality and the preparation success rate of the chromosome are remarkably improved.

Methods and techniques for controlled printing of a cell sample for karyotyping are provided. The methods can involve matrix printing using on-the-fly printing or dispensing to accurately spread cells within at least one cell sample on a surface in preparation for karyotyping, and further analysis. Advantageously, the methods result in a uniform distribution of chromosomes of the cell suspension or sample on the surface of a substrate which can be substantially discretely identified, and also provide for efficiency in a subsequent staining process and any further analysis of the stained chromosomes using a microscope or other imaging device.

The present invention belongs to the field of deep learning and medical image recognition, and provides a method for identifying HEp-2 antinuclear antibody fluorescence images based on deep learning. The main purpose is to solve the problems in the prior art that it is difficult to process high-resolution medical images and difficult to fully utilize various features in the fluorescence images, resulting in poor recognition effect of the karyotype categories of the fluorescence images. The main solutions of the present invention include: 1) collecting HEp-2 antinuclear antibody fluorescence images and corresponding labels; 2) scaling and cutting the fluorescence images; 3) designing a neural network module; 4) training the neural network; 5) inputting the HEp-2 antinuclear antibody fluorescence image whose label needs to be predicted to obtain the label of its karyotype category. The present invention can be used to identify the karyotype categories of HEp-2 antinuclear antibody fluorescence images.

The present application relates to the field of aquatic organism breeding technology, and particularly relates to a method for preparing rainbow trout tetraploid fry. The method provided by the present application comprises the following steps: obtaining fertilized eggs by dry fertilization of naturally matured rainbow trout parents, inducing the rainbow trout fertilized eggs to obtain tetraploid rainbow trout after 6.2-6.4 hours, and the duration of the induction is 10-18 minutes; after the tetraploid rainbow trout fry is obtained by induction, the chromosome ploidy of the fish is determined by using techniques such as chromosome karyotype analysis and flow cytometry DNA content determination, and the tetraploid rainbow trout fry is identified and screened for cultivation. The present application adopts a drug induction method, and controls the starting time and duration of the induction to inhibit the first mitosis time of the fertilized eggs, thereby improving the induction rate and survival rate of the tetraploid rainbow trout, and the induction rate of the tetraploid rainbow trout can reach 25-30%, and the survival rate of the fry can reach more than 90%.

The present invention discloses a karyotype recognition method, device and electronic device based on fluorescence photographs. The method includes respectively obtaining each panoramic fluorescence image of a target sample at different exposure levels based on bracketed exposure; comparing each panoramic fluorescence image and screening out abnormal panoramic fluorescence images with overexposure or underexposure; identifying the fluorescence performance information of each cell in the remaining panoramic fluorescence images to generate a karyotype recognition result. The present invention realizes image comparison and recognition of a pathological sample after fluorescence staining by means of bracketed exposure, thereby screening out panoramic fluorescence images with overexposure caused by strong positivity, ensuring the accuracy of the fluorescence performance information finally obtained based on the panoramic fluorescence images, and further ensuring the accuracy of the finally generated karyotype recognition result.

The invention belongs to the technical field of biological medicine, and discloses a safe and efficient non-gene editing neural stem cell and application thereof in treatment of nervous system related diseases. The invention provides a non-gene-edited neural stem cell (hCiPSC-NSCs) derived from human induced pluripotent stem cells, the neural stem cell is obtained by reprogramming human umbilical cord mesenchymal stem cells through a small molecule compound (such as a TGF-beta / GSK-3beta inhibitor), exogenous gene introduction is not involved, and the neural stem cell has a normal karyotype, no tumorigenicity and relatively high safety; and the efficient implantation of the non-tumorigenicity hippocampal region is realized in three months. The neural stem cell can improve the autism-like phenotype of a VPA-exposed rat by targeting the intestinal-brain axis at the same time.

The invention relates to a serum-free culture medium for in-vitro amplification of neural stem cells and a preparation method of the serum-free culture medium, and belongs to the technical field of biology. The culture medium comprises a DMEM / F12 and Neurobasic double-basal culture medium (the volume ratio is 1: 1), a bFGF / EGF / BDNF three-growth-factor combination (20 / 15 / 10ng / mL), a B27 / N2 supplement and a metabolism regulator (6g / L glucose + 0.1 mM beta-mercaptoethanol), and is prepared through the processes of stepwise dissolution of a buffer system, magnetic stirring and mixing, positive-pressure filtration and sterilization and the like. The innovation points are as follows: the double-basal culture medium synergistically balances amino acid and neurotransmitter nutrition, so that the glucose consumption rate is reduced by 25%; the three factors synergistically activate a proliferation pathway and inhibit differentiation, the amplification multiple reaches 14.8 times (7 days), and the Netin positive rate is gt; 95%; a metabolism regulation system controls lactic acid lt; the karyotype abnormality rate is 1t; and 0.3%. According to the scheme, the cost is reduced by 33%, and the method is completely free of animal-derived components and suitable for large-scale automatic production of clinical-grade neural stem cells.

The application discloses a human lung large cell neuroendocrine carcinoma cell line and application, the cell line is preserved in China typical culture preservation center, the preservation time is August 22, 2024, the preservation number is CCTCC NO:C2024278, and the culture name is human lung large cell neuroendocrine carcinoma cell strain ZX2024C.The cell line can be stably passed, can keep the biological characteristics of human lung large cell neuroendocrine carcinoma cell, no human cell cross contamination is found, and has abnormal chromosome karyotype, can form tumor in the body of nude mouse, can be applied to the preparation of lung large cell neuroendocrine carcinoma diagnostic product, screening lung large cell neuroendocrine carcinoma drug, researching lung large cell neuroendocrine carcinoma cell proliferation mechanism, constructing mammalian lung large cell neuroendocrine carcinoma animal model.

The invention relates to the technical field of cell separation and purification and cell biological detection, in particular to a method for separating and purifying fetal NRBC from peripheral blood of a pregnant woman. The invention provides a method for separating and purifying fetal NRBC from peripheral blood of a pregnant woman, which is characterized in that a LukSF and LukAB bi-component perforation toxin is designed and utilized to specifically recognize and split a CXCR1 / 2 chemotactic factor receptor and a CD11b / CD18 integrin receptor on the surface of maternal leukocyte, and the fetal NRBC is completely reserved due to lack of corresponding receptors, so that the fetal NRBC can be separated and purified. Therefore, leukocyte pollution in nucleated red blood cells of a peripheral blood fetus of a pregnant woman is efficiently removed. According to the method, the purity of the extracted fetal NRBC can be improved, the false positive of gene detection is greatly reduced, the activity and integrity of the NRBC are reserved, and the accurate diagnosis requirements of chromosome karyotype analysis, monogenic disease detection and the like are met; the method is simple to operate and low in cost, can be directly adapted to existing clinical laboratory equipment, and has large-scale popularization value.

The invention relates to the technical field of aquatic organism breeding, in particular to a rainbow trout tetraploid fry preparation method. The method provided by the invention comprises the following steps: carrying out dry fertilization on naturally mature rainbow trout parents to obtain fertilized eggs, inducing the rainbow trout fertilized eggs after 6.2-6.4 hours to obtain tetraploid rainbow trout, and carrying out induction for 10-18 minutes; after rainbow trout tetraploid offspring seeds are obtained through induction, chromosome ploidy of the rainbow trout body is measured through the technologies of chromosome karyotype analysis, flow cytometry DNA content measurement and the like, and the rainbow trout tetraploid offspring seeds are identified and screened out to be bred. According to the method, a drug induction method is adopted, the induction starting time and the induction lasting time are controlled, and the first mitotic time of fertilized eggs is inhibited, so that the induction rate and the survival rate of the tetraploid rainbow trout are improved, the induction rate of the obtained rainbow trout tetraploid can reach 25-30%, and the survival rate of the obtained rainbow trout tetraploid can reach 90% or above.

The invention discloses a construction method and application of an ovarian cell line of hapalogenys nitens. The method comprises the following steps: selecting a metamorphosis stage larva ovarian tissue, disinfecting and obtaining, and digesting for 1 hour at 26 DEG C by using collagenase I digestive juice; after the tissue block adheres to the wall, a low-osmotic-pressure complete medium is used for primary culture at the temperature of 25-27 DEG C, and the medium is a 60% diluted DMEM / F12 medium containing 10%-15% of fetal calf serum; after the cell confluence degree exceeds 70%, trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) is used for digesting for 1-2 minutes for passage, and a passage culture medium contains 30% of a cell condition culture medium; and finally performing programmed cooling and cryopreservation. According to the method, the ovarian epithelial cell line of the hapalogenys nitens is successfully established for the first time, the cell recovery survival rate is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and germplasm resource in-vitro preservation.

The invention discloses a chromosome counting method and device based on image processing and electronic equipment. The method comprises the steps that computer equipment acquires a chromosome karyotype graph; projecting each row of pixel points of the chromosome karyotype graph to obtain a first projection vector; determining a plurality of foreground regions of the chromosome karyotype graph according to the first projection vector; selecting a plurality of first foreground areas from the plurality of foreground areas, and extracting target image areas corresponding to the plurality of first foreground areas; projecting each column of pixel points of the target image area to obtain a second projection vector; determining one or more chromosome regions contained in the target image region according to the second projection vector; selecting a target chromosome region comprising the chromosomes of the target type from one or more chromosome regions contained in the target image region; and obtaining the chromosome number corresponding to the chromosome of the target type. According to the invention, the counting accuracy and efficiency of the chromosome karyotype graph can be improved.

The application relates to a chromosome image segmentation and recognition method and system based on deep learning, and relates to the technical field of medical image processing.The method comprises the following steps: performing target detection processing on original chromosome image data to output chromosome image data with labeled chromosome clusters; using a dynamic convolution kernel and performing multi-level convolution and down-sampling processing on the chromosome image data to obtain intermediate layer down-sampling feature data and final layer down-sampling feature data; combining a parameter-free attention mechanism, performing deep separable convolution processing and pixel recombination processing on the final layer down-sampling feature data to obtain deep layer feature optimization data; performing inverse convolution processing and jump connection splicing processing on the deep layer feature optimization data and the intermediate layer down-sampling feature data to output chromosome segmentation image data; and performing classification and arrangement processing on the chromosome segmentation image data to obtain a chromosome karyotype analysis result.The chromosome image segmentation and recognition method and system can achieve good segmentation and recognition effects on chromosome images.

The invention discloses a preparation method of chromosomes of tiny insects trichogramma dendrolimus, and belongs to the technical field of chromosome preparation. Comprising the following steps: (1) preparing a pre-pupa material: selecting corcyra cephalonica eggs parasitized by trichogramma dendrolimus for 4-8 days, and cutting out a trichogramma dendrolimus pre-pupa; (2) pretreatment: pretreating the pre-pupa material in a colchicine solution; (3) low-permeability treatment: carrying out low-permeability treatment on the pretreated material in a low-permeability solution; and (4) fixing, uncovering, dyeing and sealing the material subjected to the low-permeability treatment. The invention provides a simpler trichogramma dendrolimus chromosome slide preparation method, chromosome slides with clear forms can be prepared in batches, the dispersion degree of chromosomes is large, the length, centromere position, arm ratio and other characteristics of the chromosomes can be observed more clearly, the chromosome characteristics are determined through karyotype analysis, and the chromosome slide preparation method is suitable for large-scale production. And a foundation is laid for cell level research and genomics deep research of trichogramma dendrolimus.

The application provides a miscarriage risk assessment method and system of whole-exome load and serum markers, comprising: independently scoring each variation in whole-exome sequencing data to obtain a comprehensive pathogenic score; constructing a gene pathogenic load matrix; mapping the gene pathogenic load to a biological pathway to obtain a pathway comprehensive score and construct a model; solving the model by jointly optimizing the regularization strength parameters of the two-layer ridge penalty terms, outputting significant pathway features; standardizing and preprocessing serum detection data; jointly modeling the output significant pathway features and the output standardized serum data under the same logistic regression probability framework, outputting a miscarriage-related risk probability value, and performing probability stratification. The application also outputs a double-track coordination coefficient to quantify the directional consistency of genetic signals and serum signals. It is significantly superior to a single omics solution and provides a high-precision individualized risk assessment tool for recurrent miscarriage with normal karyotype.