97 results about "Karyotype" patented technology

The invention relates to the technical field of molecular biology, and particularly provides an InDel molecular marker related to the size of a lychee kernel and application of the InDel molecular marker. The InDel molecular marker is located at a site 25610680 of a fifth chromosome of a reference genome GeneBank: GCA019925255.1 of the litchi, and the InDel molecular marker is used for detecting the DNA of the litchi. The genotype of the marker in a pyrokaryotic litchi material is homozygous' GAAAAA / GAAAAA ', and the genotype of the marker in a macrokaryotic litchi material is heterozygous' G---- / GAAAAA 'or homozygous' G---- / G----'. The invention further provides a primer group for detecting the molecular marker, an effective tool is provided for molecular marker-assisted breeding of the size of the lychee kernel, and the method is expected to assist in shortening the breeding period, improving the breeding efficiency and promoting the improvement work of the lychee variety.

The invention discloses a chromosome metaphase division phase image sorting method, which belongs to the field of image processing, and comprises the following steps of: segmenting crossed and adhered chromosomes in chromosome metaphase division phase images; extracting a plurality of features from the segmented chromosome image; setting a reasonable interval of each feature, when each feature of each image is in the corresponding interval, putting the image into a set S1, when a certain feature of each image is not in the interval, putting the image into a set S2, arranging the images in the set S1 according to the number of connected domains from large to small, arranging the images in the set S2 according to the number of chromosomes from large to small, and setting the images in the set S2 according to the number of chromosomes from large to small; the length of the set S1 is added to the serial numbers of all the images in the set S2, then the two sets are combined, overall sequencing is completed, through the method, the images meeting the requirement for karyotype analysis can be automatically screened out, and manual operation is not needed; according to the sorting method, the processing speed is high, the time required for karyotype analysis is effectively shortened, and the efficiency is improved.

The invention provides a full-automatic indirect immunofluorescence interpretation method and system, a storage medium and a program product, and relates to the technical field of indirect immunofluorescence detection.The method comprises the steps that when identification information of a target sample is obtained, a plurality of sealed antigen slides are placed in slide carrying groove positions of a bearing platform in batches, and the target sample is obtained; driving the bearing platform through the transmission mechanism to sequentially move the plurality of antigen slides to the imaging area, and performing positioning and fixing operation on the antigen slides entering the imaging area by using the positioning and clamping device; sequentially carrying out multi-view scanning on the antigen slides entering the imaging area to obtain multi-view fluorescence images, and splicing the fluorescence images to generate a fluorescence panoramic image; performing feature extraction on the panoramic image, determining a fluorescence karyotype category and a target fluorescence intensity value, and determining an antibody titer according to the category, the intensity value and dilution information; and performing association mapping on the titer, the category and the panoramic image according to the identification information to generate a fluorescence interpretation result.

The invention provides a chromosome image enhancement method and system based on semantic guidance. The method comprises the following steps: S1, preprocessing; s2, outputting a deep-band probability graph, a grey-band probability graph and a shallow-band probability graph through a semantic segmentation network composed of a lightweight encoder and a multi-scale decoder; s3, implementing differential layered enhancement according to a band type: adopting local adaptive histogram equalization for a deep band, adopting central axis constraint bilateral filtering for a gray band, adopting dynamic threshold truncation and gamma correction for a shallow band, and performing weighted fusion for a transition region according to probability; s4, carrying out structure strengthening, wherein the structure strengthening comprises centromere local sharpening, stripe phase alignment, edge sensing super-resolution and overlapping region separation; and S5, performing quality evaluation based on the deep band integrity, the band stripe contrast uniformity, the SSIM and the noise density, and triggering adaptive re-enhancement if necessary. According to the scheme, the contrast ratio and details are remarkably improved while the stripe structure and the position relation are kept, and the method has the advantages of light weight, interpretability and cross-sample robustness and is suitable for being integrated into an automatic karyotype analysis process.

The invention discloses a chromosome image generation method and device for deep learning. Performing adaptive multi-scale image enhancement processing on the chromosome mid-term image to obtain an enhanced chromosome mid-term image; obtaining a chromosome karyotype arrangement diagram based on the enhanced chromosome metaphase image; generating a chromosome set based on the chromosome karyotype arrangement diagram; sequentially merging the chromosomes in the chromosome set into one image as a chromosome foreground image; fusing the chromosome foreground image with a randomly selected background image, and carrying out edge smoothing processing on the fused image to generate a random background chromosome middle-term image; wherein the random background chromosome metaphase image is used for training a target detection model established based on deep learning. According to the method, high-quality and diversified chromosome metaphase images can be generated, and when the method is used for training the deep learning model, the generalization ability and precision of the deep learning model in a target detection task are improved.

A human chronic myeloid leukemia cell line and the use thereof. The human chronic myeloid leukemia cell line is the first cell line internationally established from chronic-phase leukemia cells of chronic myeloid leukemia, and was named human chronic myeloid leukemia cell YYXY-M6, which was deposited at the China Center for Type Culture Collection (Wuhan University, Wuhan, China) on July 24, 2023, under the deposit number of CCTCC NO: C2023219. The leukemia cell line exhibits primitive cell morphology and has three karyotypes, i.e. t(6:11)(q25:q23), del(11)(q23) and normal karyotype (46, XX); is BCR-ABL gene-negative; has good in-vitro proliferation ability; can be used as cellular material for the study of the mechanism of occurrence and development of the chronic phase of chronic myeloid leukemia, from the chronic phase thereof to the blastic phase thereof, and of BCR-ABL gene-negative chronic myeloid leukemia, and for the in-vitro study of individualized treatment; and can also be used for both in-vitro and in-vivo studies of drug screening and evaluation for the chronic phase of chronic myeloid leukemia, from the chronic phase thereof to the blastic phase thereof, and BCR-ABL gene-negative chronic myeloid leukemia, providing guidance for clinical medication.

The invention discloses a method for preparing a nanocrystal nucleus type early strength agent by using an in-situ growth technology and application of the nanocrystal nucleus type early strength agent, and belongs to the technical field of concrete admixtures. The method comprises the following three steps: preparing an in-situ growth calcium source, preparing a silane modified polycarboxylic acid dispersing agent and preparing a nano hydrated calcium silicate crystal nucleus early strength agent. According to the preparation method, nano calcium oxide grows on a growth carrier by utilizing high dispersity of the growth carrier in water, so that the dispersing capacity of the nano calcium oxide in water is improved, in addition, a polycarboxylic acid dispersing agent is modified by utilizing amino silane, and a firm isolating layer with Si-O-Si as a framework is formed by utilizing the fact that silane and hydroxyl on the surface of a hydrated calcium silicate crystal nucleus can be subjected to dehydration condensation; the crystal nucleus growth is inhibited. Therefore, the nano C-S-H crystal nucleus dispersing agent has high dispersity and high stability.

The invention discloses a panda immortalized kidney cell line GPK-1 as well as a preparation method and application thereof, and belongs to the technical field of immortalized cells. The preparation method comprises the following steps: (1) collecting kidney tissues of dead pandas, and soaking the kidney tissues in a protective solution; (2) carrying out two-step digestion on the kidney tissue treated in the step (1); and (3) carrying out culture passage on the digested cells, then infecting the passage cells with lentiviruses carrying neomycin resistance genes and SV40-LT genes, and then screening to establish the panda immortalized kidney cell line. The invention provides a technology for efficiently separating and culturing panda nephrogenic cells, and establishes an immortalized panda nephrogenic cell line GPK-1. Compared with primary kidney cells of pandas, the GPK-1 retains normal karyotype and related molecular characteristics and is remarkably improved in the aspects of cell passage times, proliferation efficiency and transfection efficiency.

The application discloses a construction method and application of an ovary cell line of Quasipaa spinosa. The method comprises the following steps: selecting metamorphosis period larva ovary tissue, disinfecting and obtaining the ovary tissue, and then using collagenase I digestion solution to digest the ovary tissue at 26 DEG C for 1 hour; after the tissue block adheres to the wall, using a low-osmotic-pressure complete culture medium to carry out primary culture at 25-27 DEG C, wherein the culture medium is 60% diluted DMEM / F12 culture medium containing 10%-15% fetal bovine serum; when the cell confluence degree is higher than 70%, using trypsin-EDTA to digest the cells for 1-2 minutes to subculture, and the subculture culture medium contains 30% cell conditional culture medium; and finally, the cells are cryopreserved through programmed cooling. The ovary epithelial cell line of Quasipaa spinosa is successfully established for the first time, the survival rate of the cells after recovery is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and in-vitro conservation of germplasm resources.

The invention discloses a chromosome karyotype analysis method and system, and relates to the technical field of image recognition, and the method comprises the following steps: S1, obtaining to-be-analyzed chromosome image data, and marking the to-be-analyzed chromosome image data to obtain a to-be-analyzed image data set; s2, introducing an improved module to perform network reconstruction on the YOLOv11 network model to obtain a chromosome detection model; s3, training a chromosome detection model through the to-be-analyzed image data set, and performing performance evaluation to obtain a target chromosome detection model; and S4, analyzing the real-time chromosome image data through the target chromosome detection model, and outputting an analysis result. According to the scheme, the detection capability of the model on chromosomes in small-target, abnormal and complex scenes is enhanced by utilizing an improved module, and the problems of low accuracy and poor robustness in the prior art are solved, so that efficient and accurate chromosome karyotype analysis is realized, and clinical and occupational health examination requirements are met.

The invention discloses a slide preparation method for rapidly obtaining root tip chromosomes of Shenghua and application, and belongs to the technical field of chromosome slide preparation. The flaking method comprises the following steps: carrying out cuttage water culture on node vines at the overground part of the ginseng and the sweet potatoes, and inducing to generate adventitious roots; the root tips of the adventitious roots are taken and placed in a pretreatment solution to be pretreated; the pretreated root tips are placed in a fixing solution to be fixed; sequentially carrying out acidolysis and enzymolysis on the fixed root tips; taking root tip meristematic zone tissues after acidolysis and enzymolysis, placing the root tip meristematic zone tissues on a glass slide, dyeing, tabletting, and placing on a heating plate for heating; according to the flaking method disclosed by the invention, the division phase metaphase cells with clean flaking background, clear chromosome morphological structure, obvious chromosome overflow mark and good dispersion can be rapidly obtained, the chromosome number statistics and karyotype analysis are convenient, and the flaking method has important significance on ploidy identification, variety identification, genetic relationship identification and the like of the sweet potatoes.

A chromosome image segmentation identification method and system based on deep learning relates to the technical field of medical image processing, and comprises the following steps: performing target detection processing on original chromosome image data, and outputting chromosome image data calibrated with chromosome clusters; performing multi-level convolution and down-sampling processing on the chromosome image data by using a dynamic convolution kernel to obtain middle-layer down-sampling feature data and final-layer down-sampling feature data; in combination with a parameter-free attention mechanism, performing depth separable convolution processing and pixel recombination processing on the sampling feature data under the final layer to obtain deep feature optimization data; performing deconvolution processing and jump connection splicing processing on the deep feature optimization data and the intermediate layer down-sampling feature data, and outputting chromosome segmentation image data; classifying and arranging the chromosome segmentation image data to obtain a chromosome karyotype analysis result; the chromosome image segmentation and identification method and system have good segmentation and identification effects on the chromosome image.

The application belongs to the technical field of biology, and discloses a use of antisense nucleotide of piR-bmo-796514 nucleotide for preparing a medicine for inhibiting Bombyx mori nuclear polyhedrosis virus, the antisense nucleotide of the piR-bmo-796514 nucleotide is mainly used for inhibiting replication of the Bombyx mori nuclear polyhedrosis virus in ovary cells of the Bombyx mori, and a sequence of the piRNA nucleotide is shown as SEQ ID NO:1; in addition, the application also discloses the antisense nucleotide of the piR-bmo-796514 nucleotide, and an antisense nucleotide sequence is shown as SEQ ID NO:2.

The invention belongs to the technical field of agricultural biology, and particularly relates to application of tunicamycin to prevention and treatment of bombyx mori nuclear polyhedrosis viruses. Bioinformatics analysis, cell transfection gene overexpression, gene knockout, apoptosis detection and other test methods find that bombyx mori GPT (BmGPT) plays an important role in BmNPV proliferation, at least part of BmGPT knock-down inhibits BmNPV proliferation by enhancing BmN cell apoptosis, the GPT inhibitor chicamycin can inhibit BmNPV proliferation in bombyx mori cells, and the BmNPV proliferation inhibitor can inhibit BmNPV proliferation in bombyx mori cells. And a new means is provided for prevention and treatment of the BmNPV of the silkworms.

The invention belongs to the field of biological detection, and particularly relates to a blood treating fluid and a method for performing karyotype analysis by using the treating fluid. The treating fluid prepared by the invention comprises a treating fluid A and a treating fluid B. The treating fluid can be used for treating umbilical cord blood of a fetus at the early pregnancy stage, so that the interference of red blood cells can be reduced, and rapid proliferation of lymphocytes can be induced. The umbilical cord blood treated by the treatment liquid is subjected to chromosome karyotype analysis, can be refined into number abnormality or structure abnormality typing, and has important significance for clinical prenatal diagnosis.

The present invention belongs to the technical fields of molecular biology and molecular breeding, and particularly relates to SNP molecular markers related to Bombyx mori nucleopolyhedrovirus (BmNPV) resistance and their applications. The SNP molecular markers include the combination of the Chr3-746 marker and the Chr27-5071 marker; the Chr3-746 marker is located at the 14951064th position of the 3rd chromosome sequence of the Bombyx mori p50T genome, and the base polymorphism is G / A; the Chr27-5071 marker is located at the 9462596th position of the 27th chromosome sequence of the Bombyx mori p50T genome, and the base polymorphism is C / G. The SNP molecular markers of the present invention can effectively distinguish BmNPV-resistant and -susceptible Bombyx mori, reduce the false positive rate of detection, improve the accuracy rate, and can be screened quickly and in a high-throughput manner, solve the technical problems of traditional Bombyx mori breeding, and accelerate the breeding of BmNPV-resistant Bombyx mori varieties.

The present application relates to medical chromosome karyotype image processing technical field, provide a kind of semi-automatic labeling method of chromosome karyotype image data.The method includes: obtaining the key information required for automatic labeling;Based on the key information, construct and generate a labeling file according to the specified data format, and extract the coordinate point information of the effective contour of the chromosome required for automatic labeling;Manual annotation is completed using the labeling tool to the unannotated chromosome.The present application automatically annotates the internal objects of chromosome karyotype image by image processing method, and checks and annotates the annotation results manually, shortens the time cost spent on labeling, greatly improves the labeling efficiency.

The present invention discloses a giant panda immortalized kidney cell line GPK‑1, a preparation method and a use thereof, and belongs to the field of immortalized cell technology. The preparation method is as follows: (1) collecting kidney tissue of a dead giant panda and immersing it in a protective solution; (2) performing two-step digestion on the kidney tissue treated in step (1); (3) culturing and propagating the digested cells, and then infecting the propagated cells with a lentivirus carrying a neomycin resistance gene and an SV40‑LT gene, and then screening and establishing a giant panda immortalized kidney cell line. The present invention provides a technology for efficiently isolating and culturing giant panda kidney-derived cells, and establishes an immortalized giant panda kidney-derived cell line GPK‑1. Compared with primary giant panda kidney cells, GPK‑1 retains normal karyotype and related molecular characteristics, and has been significantly improved in terms of cell passage times, proliferation efficiency and transfection efficiency.

The invention discloses an SSR molecular marker for screening nuclear cracking resistance characters of peaches and application of the SSR molecular marker, and relates to the technical field of genetic breeding of fruit trees, the SSR molecular marker is named as PpCPK12-SSR, and the nucleotide sequence of the SSR molecular marker is shown as SEQ ID NO.1. The invention also provides a specific primer pair for amplifying the marker, the nucleotide sequence of an upstream primer of the specific primer pair is shown as SEQ ID NO.2, and the nucleotide sequence of a downstream primer of the specific primer pair is shown as SEQ ID NO.2. The nucleotide sequence of the downstream primer is as shown in SEQ ID NO.3. Through PCR (polymerase chain reaction) amplification and electrophoresis detection, according to the size of an amplified fragment, the anti-nuclear-cleavage genotype of the peach can be quickly and accurately identified in the seedling stage of the peach: an individual carrying a 115bp fragment is an anti-nuclear-cleavage type; the defects that traditional field phenotype identification is long in period, low in efficiency and affected by the environment are overcome, an efficient molecular marker auxiliary selection tool is provided for peach nuclear cracking resistance breeding, and the breeding process can be remarkably accelerated.

The invention discloses a peripheral blood lymphocyte culture standardization process, and relates to the technical field of cell culture, and the peripheral blood lymphocyte culture standardization process comprises the following steps: S1, blood sample planting; s2, cell culture; s3, harvesting the cells; s4, wafer dropping; s5, carrying out banding treatment; and S6, scanning and analyzing. The standardized process intends to standardize the whole peripheral blood chromosome preparation process, manual errors are reduced through instrument operation, experience judgment is replaced with specific numerical values, the standard operation process can avoid the slide preparation gap caused by human factors, then the peripheral blood chromosome karyotype analysis resolution is improved, a guarantee is provided for the reliability of clinical results, and the method is suitable for large-scale popularization and application. The step is the key of cytogenetics research, and detailed cell and chromosome data are provided; the standardized process aims to ensure that high-quality data output can be achieved in the culture, treatment and analysis process of the lymphocytes, so that the final analysis result is accurate and reliable.

The invention discloses an oligonucleotide-coated fluorescence in-situ hybridization probe for specifically recognizing a tartary buckwheat Chr1-4 chromosome and application, and belongs to the field of cytological markers. The method comprises the following steps: screening a single-copy oligonucleotide sequence of Chr1-4 from a tartary buckwheat whole genome by using Chorus2 software; filtering and removing repetitive sequences to obtain a Chr1-4 chromosome probe sequence library; screening a specific single copy oligonucleotide sequence of the Chr1-4 chromosome; and adding specific primers at the two ends of each single copy oligonucleotide sequence of Chr1-4, and combining the probes into a buckwheat Chr1-4 Oligo-Painting probe mixing pool. By utilizing the constructed Oligo-Painting probe mixing pool and a fluorescence in-situ hybridization technology, accurate identification and karyotype analysis of the buckwheat chromosomes can be realized, the number, morphology, heritable variation and other structural characteristics of the chromosomes can be known, and an important technical support is provided for cytological analysis of buckwheat crossbreeding.

The invention provides a novel human distal bile duct cancer cell line with TP53 missense mutation and application, the novel human distal bile duct cancer cell line CBC3T-3 is established, the cell line is preserved in the China Center for Type Culture Collection (the preservation number is CCTCC NO: C202555), the uniqueness and stability of the cell line are proved through STR typing and karyotype analysis, and the TP53 missense mutation novel human distal bile duct cancer cell line has the advantages that the TP53 missense mutation novel human distal bile duct cancer cell line CBC3T-3 can be used for preparing the TP53 missense mutation novel human distal bile duct cancer cell line CBC3T-3; and a plurality of driver gene mutations including TP53 missense mutation are carried. The CBC3T-3 has strong proliferation, invasion and migration capabilities, has high tumor formation rate in immunodeficient mice, is resistant to cis-platinum and sensitive to paclitaxel and gemcitabine, and provides an experimental basis for selection of clinical chemotherapy regimens. According to the model, the TP53 missense mutation type distal bile duct cancer in-vitro model is successfully established, and a key experimental platform is provided for deeply researching the drug resistance mechanism of the TP53 missense mutation type distal bile duct cancer and developing an individualized treatment strategy aiming at the subtype of the TP53 missense mutation type distal bile duct cancer.

The invention discloses a quality control product for noninvasive prenatal detection of fetal chromosome trisomy syndrome and a preparation method thereof, the quality control product comprises a positive quality control product and a negative quality control product, the positive quality control product is obtained by adding nucleic acid fragments of trisomy syndrome genome DNA into background plasma; the negative quality control product is obtained by adding nucleic acid fragments of normal karyotype genome DNA into background plasma; the length of the nucleic acid fragment is 130 to 200 bp; the background plasma is peripheral blood plasma of normal karyotype and pregnant fetus women. According to the invention, peripheral blood plasma of a pregnant woman with normal karyotype and pregnant fetus is used as background plasma, a DNA extraction correction coefficient is introduced, and nucleic acid fragments of trisomatic negative and positive genomes 130-200bp with sex chromosome XY are added to prepare the quality control product, so that the quality control product is more accurate and reliable, and is more suitable for pregnant woman plasma; and whether each index of the noninvasive antenatal detection kit meets the technical requirements of a product or not can be reflected more completely and truly.

The invention provides a gene with an antiviral effect and application thereof, the nucleotide sequence of an open reading frame of the gene is SEQ ID NO: 2, and the amino acid sequence of a coding protein of the gene is SEQ ID NO: 1. The invention also provides a method for increasing the sensitivity of the beet armyworm to the beet armyworm nuclear polyhedrosis virus, which is used for reducing the expression quantity of the gene in the beet armyworm. According to the invention, a gene with an antiviral effect is screened from an important agricultural pest beet armyworm. The sequence structure and tissue expression mode of the gene are analyzed, incremental expression and RNA interference technologies are utilized to prove that the gene has an anti-SeMNPV effect, a new target is provided for better utilizing baculovirus to prevent and control beet armyworms in production, and the gene has a good application prospect.

The invention provides an improved euchiloglanis kishinouyei chromosome preparation method, which comprises the following steps: gradually raising the temperature and injecting PHA twice: in the euchiloglanis kishinouyei temporary culture process, raising the water temperature from 18 DEG C to 20 DEG C within 12 hours, injecting a first needle of PHA, raising the temperature to 22.5-23 DEG C after 12 hours, injecting a second needle of PHA, and keeping the water temperature at 22.5-23 DEG C unchanged; after the PHA is injected for the second time for 3 hours, colchicine is injected; sampling: washing the head and kidney in normal saline, and collecting cells; 0.5% of KCl is used for hypotonic treatment; fixing with a Carnoy's fixing solution, and centrifuging; dropping by a cold dropping method and naturally drying; and carrying out Giemsa dyeing and flaking. According to the method, the euchiloglanis kishinouyei somatic cell proliferation is promoted through heating and twice PHA injection, so that the metaphase division phase is increased, the form is clear and dispersed, and the karyotype quality and the preparation success rate of the chromosome are remarkably improved.

Methods and techniques for controlled printing of a cell sample for karyotyping are provided. The methods can involve matrix printing using on-the-fly printing or dispensing to accurately spread cells within at least one cell sample on a surface in preparation for karyotyping, and further analysis. Advantageously, the methods result in a uniform distribution of chromosomes of the cell suspension or sample on the surface of a substrate which can be substantially discretely identified, and also provide for efficiency in a subsequent staining process and any further analysis of the stained chromosomes using a microscope or other imaging device.

The present invention belongs to the field of deep learning and medical image recognition, and provides a method for identifying HEp-2 antinuclear antibody fluorescence images based on deep learning. The main purpose is to solve the problems in the prior art that it is difficult to process high-resolution medical images and difficult to fully utilize various features in the fluorescence images, resulting in poor recognition effect of the karyotype categories of the fluorescence images. The main solutions of the present invention include: 1) collecting HEp-2 antinuclear antibody fluorescence images and corresponding labels; 2) scaling and cutting the fluorescence images; 3) designing a neural network module; 4) training the neural network; 5) inputting the HEp-2 antinuclear antibody fluorescence image whose label needs to be predicted to obtain the label of its karyotype category. The present invention can be used to identify the karyotype categories of HEp-2 antinuclear antibody fluorescence images.

The present application relates to the field of aquatic organism breeding technology, and particularly relates to a method for preparing rainbow trout tetraploid fry. The method provided by the present application comprises the following steps: obtaining fertilized eggs by dry fertilization of naturally matured rainbow trout parents, inducing the rainbow trout fertilized eggs to obtain tetraploid rainbow trout after 6.2-6.4 hours, and the duration of the induction is 10-18 minutes; after the tetraploid rainbow trout fry is obtained by induction, the chromosome ploidy of the fish is determined by using techniques such as chromosome karyotype analysis and flow cytometry DNA content determination, and the tetraploid rainbow trout fry is identified and screened for cultivation. The present application adopts a drug induction method, and controls the starting time and duration of the induction to inhibit the first mitosis time of the fertilized eggs, thereby improving the induction rate and survival rate of the tetraploid rainbow trout, and the induction rate of the tetraploid rainbow trout can reach 25-30%, and the survival rate of the fry can reach more than 90%.

The present invention discloses a karyotype recognition method, device and electronic device based on fluorescence photographs. The method includes respectively obtaining each panoramic fluorescence image of a target sample at different exposure levels based on bracketed exposure; comparing each panoramic fluorescence image and screening out abnormal panoramic fluorescence images with overexposure or underexposure; identifying the fluorescence performance information of each cell in the remaining panoramic fluorescence images to generate a karyotype recognition result. The present invention realizes image comparison and recognition of a pathological sample after fluorescence staining by means of bracketed exposure, thereby screening out panoramic fluorescence images with overexposure caused by strong positivity, ensuring the accuracy of the fluorescence performance information finally obtained based on the panoramic fluorescence images, and further ensuring the accuracy of the finally generated karyotype recognition result.

The invention belongs to the technical field of biological medicine, and discloses a safe and efficient non-gene editing neural stem cell and application thereof in treatment of nervous system related diseases. The invention provides a non-gene-edited neural stem cell (hCiPSC-NSCs) derived from human induced pluripotent stem cells, the neural stem cell is obtained by reprogramming human umbilical cord mesenchymal stem cells through a small molecule compound (such as a TGF-beta / GSK-3beta inhibitor), exogenous gene introduction is not involved, and the neural stem cell has a normal karyotype, no tumorigenicity and relatively high safety; and the efficient implantation of the non-tumorigenicity hippocampal region is realized in three months. The neural stem cell can improve the autism-like phenotype of a VPA-exposed rat by targeting the intestinal-brain axis at the same time.