25 results about "Epigenetics" patented technology

The invention relates to the technical field of cosmetics, and discloses a composition and application thereof to preparation of a reagent for intervening lip aging. The composition comprises Inca inchi seed oil, stearyl glycyrrhizinate and a myrothamnus flabellifolia leaf and stem extract, wherein the mass ratio of the Inca inchi seed oil to the stearyl glycyrrhizinate to the myrothamnus flabellifolia leaf and stem extract is (1-15): 1: (0.2-10). According to the composition, through scientific compounding and a multi-target epigenetics mechanism, the function of tough lip blood vessels is improved and maintained, meanwhile, the epidermis is repaired, and lip rejuvenation is achieved through the dual effects of the epidermis and the blood vessels.

The invention discloses a whole genome methylation detection method based on MeDIP-MSRE, and belongs to the technical field of epigenetics detection. According to the method, methylation immunoprecipitation sequencing and a methylation sensitive restriction enzyme technology are innovatively combined, firstly, a methylation specific antibody is used for conducting immunoprecipitation on sample DNA, and whole genome methylation fragments are enriched; then carrying out enzyme digestion on the enriched product by adopting methylation sensitive restriction enzyme, specifically removing an unmethylated DNA region, and reserving a complete methylation sequence; and finally, constructing a methylation map through high-throughput sequencing. According to the method, traditional hydrosulfite chemical conversion is not needed, DNA damage and base conversion deviation caused by the traditional hydrosulfite chemical conversion are avoided, meanwhile, high sensitivity of MeDIP and high specificity of methylation sensitive restriction endonuclease are fused, and the fidelity, sensitivity and specificity of detection are remarkably improved.

Described herein are methods and compositions related to methylation-based determination and characterization of subjects afflicted with various stages of cancer, including lung cancer and non-small cell lung cancer (NSCLC). Osimertinib is one of the most common precision therapies in non-small cell lung cancer but nearly all patients eventually progress due to somatic mutations and cellular plasticity that bypass the treatment. Defining the cellular plasticity state with epigenetics will help physicians know when to switch therapies.

The application belongs to the technical field of bioinformatics, and discloses an epigenetic age assessment system and method based on machine learning; comprising: collecting methylation data of a user in real time; screening methylation feature data from the methylation data and compressing the methylation feature data into hidden feature data; obtaining historical feature data and constructing an age assessment model; inputting the hidden feature data obtained in real time into the age assessment model, predicting an epigenetic age, and marking the epigenetic age as a predicted age; systematically perturbing the predicted age, calculating a prediction error value of each simulated age, taking a simulated age corresponding to a minimum prediction error value as a corrected age; calculating a relative age, and taking a mean value of the relative age and the corrected age as an epigenetic age of the user; the application can improve the adaptability and accuracy of the model on different individuals and age groups, thereby improving the accuracy and timeliness of epigenetic age assessment.

The application discloses a whole-genome DNA cytosine hydroxymethylation modification single-base precision library construction method, relates to the technical field of genomics, epigenetics and molecular biology, and contains the following steps: 5hmC labeling reaction, click chemistry reaction, sample DNA fragmentation, purification of the fragmentation product through magnetic beads, 5hmC fragmented DNA capture, washing of the capture product, reduction reaction, APOBEC enzyme deamination reaction, purification of the deamination product, double-strand conversion reaction, end repair and 3' end A addition reaction, linker ligation, purification of the ligation product and double sorting, library amplification and library purification. The library construction method integrates low data volume, single-base precision and conventional double-strand library construction kit in one under the premise of ensuring data authenticity, and has the technical advantages of high universality and highly repeatable data.

The application discloses a kind of gene markers in esophagus, stomach, intestine multiple cancer early screening application, early screening model construction method and detection device, belong to the early non-invasive detection technical field of digestive tract tumor.It establishes a new type of multiple cancer screening system by analyzing the whole genome characteristics of circulating free DNA in peripheral blood.Based on low-depth whole genome sequencing data, three dimensions of molecular markers are detected: genome copy number variation pattern, DNA fragment distribution characteristics of specific length and epigenetic signals of transcription initiation region.Advanced converter neural network architecture is used, and the model can efficiently capture the complex feature correlation in the whole genome range through its unique self-attention mechanism.The model design specially considers the particularity of genomic data, and introduces an adaptive position coding system to accurately reflect the spatial distribution relationship of DNA fragments on the chromosome.The system can still maintain excellent detection performance at very low sequencing depth.

This invention provides an application of a specific DNA molecule in identifying the authentic quality of Dendrobium officinale. The specific DNA molecule includes the sequence shown in SEQ ID NO.1. Based on the principles of epigenetics, this invention identifies environment-associated molecular markers in Dendrobium officinale grown under different environments. Using these markers for quantitative real-time PCR, the growth environment of Dendrobium officinale materials can be distinguished within one day. The procedure is simplified, easy to operate, and cost-effective, providing an objective and qualitative detection method for the authentic quality of Dendrobium officinale.

This invention relates to the fields of traditional Chinese medicine and anti-aging technology. The invention provides a scientifically formulated traditional Chinese medicine composition based on epigenetic guidance, its preparation method, and its application. The composition is made from the following raw materials in parts by weight: ginseng 3-12 parts, cordyceps 3-12 parts, polygonatum 2-8 parts, ganoderma lucidum 2-10 parts, mulberry fungus 2-6 parts, pine needles 1-6 parts, epimedium 2-8 parts, salvia miltiorrhiza 3-10 parts, bletilla striata 1-5 parts, cistanche deserticola 2-6 parts, rosemary 1-5 parts, and banana peel 1-6 parts. The composition is designed to comprehensively consider aging-related oxidative stress, energy metabolism regulation, and multi-component intervention needs, and is constructed in conjunction with epigenetic regulatory directions, reflecting the standardized design concept of scientifically formulated traditional Chinese medicine. The composition of this invention has the characteristics of clear composition, feasible preparation process, controllable quality parameters, and verifiable efficacy indicators.

The invention relates to an immunofluorescence method and kit for detecting zebra fish heart DNA methylation level, and belongs to the technical field of biological detection. The immunofluorescence method provided by the invention comprises the following steps: exposing a zebrafish embryo to a substance to be detected, dissecting a heart sample of the zebrafish embryo and carrying out fixation, membrane permeation, protease K digestion, hydrochloric acid DNA denaturation and neutralization treatment, and then carrying out sealing, incubation of a 5-methylcytosine primary antibody and a fluorescently labeled secondary antibody, cell nucleus staining and fluorescence detection. And finally, analyzing and evaluating the DNA methylation level through fluorescence imaging. According to the method disclosed by the invention, through a combined step of protease K and hydrochloric acid denaturation treatment, the binding efficiency to the antibody is remarkably improved, so that high-sensitivity and low-background detection on the methylation level of the whole DNA in a zebra fish micro heart sample is realized; the detection kit provided by the invention can be widely applied to epigenetics evaluation of toxicity of environmental pollutants.

Disclosed herein is a novel DNA methylation-based tool designed to predict intrinsic capacity, which encompasses the sum of an individual's physical and mental capacities. A computational model was developed that, given the DNA methylation levels of 91 Cytosine-phosphate-Guanine (CpGs) sites in the blood, calculates an estimate of intrinsic capacity.

The application discloses a data analysis method in the field of epigenetics: a quantitative analysis method of RNA 5-methylcytosine modification on splicing isomers. The method can solve the problem of how to quantitatively analyze RNA 5-methylcytosine modification on the level of gene splicing isomers, and the technical points include: converting the data set generated by the RNA-BSseq sequencing technology into a virtual RNA-Seq data set and marking the data in which RNA 5-methylcytosine modification occurs; estimating the expression amount of the splicing isomer in the virtual data set, and calculating the mean and variance thereof by using the bootstrap technology; estimating the RNA 5-methylcytosine modification level of the splicing isomer, and calculating the mean and variance of the RNA 5-methylcytosine modification level of the splicing isomer by using the Delta technology; and based on the normal distribution, testing whether there is a significant difference in the estimated RNA 5-methylcytosine modification level of the splicing isomer under different conditions. The application helps to reveal the regulation mechanism of RNA 5-methylcytosine modification in physiological processes and pathological changes.

The application discloses a solution for separating fat cell nucleus and cytoplasm, and the solution comprises solution 1 and solution 2; wherein the solution 1 comprises 8-12 mM hydrochloride buffer, 0.03-0.15% Tween-20, 8-12 mM sodium chloride, 2-4 mM magnesium chloride, 0.8-2% bovine serum albumin and 0.4-0.6% ethyl phenyl polyethylene glycol; the solution 2 comprises 8-12 mM hydrochloride buffer, 8-25 mM sodium chloride, 1-15 mM magnesium chloride and 0.8-2.5% bovine serum albumin; the percentage is volume percentage. The application can realize the research on all cell types of fat tissues, greatly reduces the required amount of fat tissues and improves the possibility of carrying out single cell research. The application can be used for both transcriptome research and epigenetic research.

To provide a defined culture medium for isolating stratified epithelial stem cells and stably maintaining the epigenetics of the stratified epithelial stem cells during multiple passages, and to provide clonal stratified epithelial stem cells isolated using the defined culture medium.SOLUTION: And a defined culture medium comprising each of ROCK (Rho kinase) inhibitors, mitogenic growth factors, insulin or IGF, TrkA inhibitors, and Oct4 activators, and comprising at least one of VEGF inhibitors, tyrosine kinase inhibitors, and / or FGF10 or FGF10 agonists, and optionally further comprising TGF β signaling pathway inhibitors and / or bone morphogenetic proteins (BMP) antagonists, wherein the defined culture medium supports epigenetically stable growth and proliferation of stem cells of stratified epithelial tissue origin in culture.SELECTED DRAWING: Figure 1

The invention relates to the cross technical field of bioinformatics and epigenetics, and particularly discloses a method for rapidly identifying plant imprinting genes based on multi-species sequence conservation.The method comprises the steps that a whole genome gene set of target plant species is obtained; selecting reference plant species with different genetic relationships with the target plant species, obtaining an optimal homologous gene of each gene of the target plant species in the reference plant species, and constructing a multi-species homologous sequence set; performing multi-sequence comparison on the multi-species homologous sequence set, and calculating a conservative score of each target gene based on a comparison result; and sorting and screening the target genes based on the conservative scores, and preliminarily identifying the genes with the conservative scores higher than a set threshold as candidate plant imprinting genes. According to the method, high-throughput and low-cost screening and prediction of potential plant imprinting genes are realized by utilizing the characteristic that the plant imprinting genes have higher conservative property in an evolution process and calculating cross-species conservative property scores of the genes.

The invention relates to the technical field of stem cell culture, in particular to an AI algorithm for evaluating the growth state of mesenchymal stem cells, which comprises the following steps: acquiring mesenchymal stem cell image data, and inputting related basic information; extracting morphological characteristic data of the cells from the image data by utilizing a stem cell culture technology and a deep learning algorithm, and judging whether the cells have pollution, degeneration or abnormal morphology; based on the cell morphological characteristic data, analyzing the growth stage of the cells, and adjusting culture conditions according to the characteristics of the stage; collecting epigenetics data of stem cells, predicting cell proliferation and differentiation trends by using a deep learning model in combination with cell morphological characteristic data and cell growth stage analysis results, and adjusting culture conditions; and monitoring metabolite data in the stem cell culture solution in real time, evaluating the health state of the cells by combining the metabolite data, the cell morphological characteristic data and the cell cycle analysis result, and judging whether the problem of abnormal metabolism or decline exists or not.

The invention provides a biological microsphere and a preparation method thereof, and a multi-skin appendage in-situ regeneration method, the biological microsphere is constructed by loading a small molecule compound combination and a protein by using a lipid carrier sphere, the small molecule compound combination and the protein are integrated, and the lipid carrier sphere is used for slow release, so that epigenetics can be deeply changed; the method has the advantages of maintaining cell proliferation, activating hair papilla cell and sweat gland cell fate, greatly promoting the cell fate conversion efficiency, achieving cell reprogramming, being safe, efficient and easy to operate, and avoiding the safety risk of gene editing, thereby effectively solving the problems of wound repairing, scar weakening and skin functional regeneration.

The invention belongs to the technical field of biological fermentation, and discloses a method for producing pleosporoneF by using endophytic fungi through epigenetics modification. The fermentation method comprises the following steps: adding an activated strain Aspergillus fumigasus into a PDB culture medium with the nicotinamide content of 50-500 [mu] M, culturing for 3-7 days under the conditions of 25-30 DEG C and 150-200 rpm / min to obtain a seed solution, inoculating the seed solution into the PDB culture medium with the nicotinamide content of 50-500 [mu] M for mass fermentation, culturing for 5-15 days under the conditions of 25-30 DEG C and 150-200 rpm / min, extracting a fermentation solution for three times by using ethyl acetate, merging ethyl acetate extracting solutions, and spin-drying to obtain a finished product. The method comprises the following steps: removing impurities by using a silica gel column, and recrystallizing to obtain a target product. According to the method disclosed by the invention, the pleosporone F is produced by utilizing the endophytic fungi Aspergillus fumigasus, so that the fermentation time is shortened, the technological process is simpler and more environment-friendly, the production and purification processes are simple to operate, and the production cost can be effectively reduced.

The application provides application of specific DNA molecules in identification of genuine quality of Dendrobium, and the specific DNA molecules comprise a sequence shown in SEQ ID NO. 1. According to the principle of epigenetics, the environment-related molecular markers are found in Dendrobium grown in different environments, and the markers are used for fluorescence quantitative PCR, so that the growth environment of Dendrobium materials can be distinguished within one day, the steps are simplified, the operation is easy, the cost is controllable, and an objective qualitative detection method for the genuine quality of Dendrobium is provided.

This invention discloses a method for constructing an age prediction model based on DNA methylation microhaplotypes, belonging to the field of bioinformatics. Through bioinformatics analysis of methylation data from multiple whole blood samples, DNA methylation microhaplotype sites for accurate age prediction are selected using the sparse group Lasso algorithm. Further, a convolutional neural network is used for age regression modeling, and the stability and accuracy of the model are verified in multiple independent cohorts. This model provides a novel and effective technical means for age prediction in epigenetics, with high scalability and application prospects.

The invention belongs to the technical field of biological detection equipment, and particularly relates to an epigenetic detection micro-fluidic chip which comprises a sample injection layer, a reaction detection layer, a signal conduction layer and a substrate supporting layer which are sequentially arranged from top to bottom, and all the layers are packaged and fixed into an integrated chip body through laser welding. The sample injection layer is integrated with a single-channel sample injection port, a micro filtering module and an exclusive reagent pre-storage cavity, the reaction detection layer is provided with a plurality of groups of targeted reaction chambers, a nanogold-graphene composite array modified by a specific probe is fixed on the inner wall of the cavity body of each targeted reaction chamber, and the reaction detection layer is provided with a plurality of groups of targeted reaction chambers; through multi-layer function integrated design, targeted nano array modification and process innovation, the detection specificity and efficiency are remarkably improved, and rapid, accurate and integrated detection of the Alzheimer's disease epigenetics modification marker is achieved.

This invention belongs to the field of epigenetics, and specifically relates to a maize gene. Jmj1 Applications in regulating maize photoperiod. (Gene) Jmj1 The coding region sequence is shown in SEQ ID No. 1, knockout Jmj1 Arabidopsis thaliana strains with genes ( Jmj1 The bolting time of Zm-KO was significantly advanced, indicating an earlier flowering initiation process; Jmj1 Multiple knockout lines (such as Zm‑Jmj1 The tasseling time of ko*1, ko*2, and ko*10 / 11 / 12 was significantly advanced, and the development process of the male spike was significantly accelerated; enzyme activity detection proved that this gene can significantly reduce the modification level of histone H3K36me3. This invention reveals Jmj1 The key role of genes in the regulation of maize photoperiod provides crucial gene resources for breeding early-maturing maize varieties adapted to different photoperiod environments, and has significant application value and industrialization prospects in maize molecular breeding and agricultural quality improvement and yield enhancement.

The present invention relates to engineered TET enzymes useful for epigenetics and next generation sequencing (NGS), and more particularly to sequencing methods such as Tet assisted pyridine borane sequencing (TAPS).

The application provides a composite biomaterial loaded with multi-mode reprogramming therapeutic agents, a preparation method thereof and application thereof in treating osteoarthritis, and belongs to the technical field of biological medical materials, drug delivery, regenerative medicine and epigenetics. The composite biomaterial comprises sodium isoamyl barbital, an epigenetic reprogramming small molecule VC6TF and a lipid nanoparticle encapsulating a reprogramming factor mRNA. The composite material has a precise construction idea and a systematic in-vivo and in-vitro performance and safety evaluation system, so that it can treat osteoarthritis by precisely reversing the epigenetic age of joint cells.

This invention discloses a method, system, and storage medium for detecting canine biological age based on simplified methylation sequencing, belonging to the fields of biotechnology, animal epigenetics, and bioinformatics. This invention obtains sample DNA through non-invasive saliva sampling, uses simplified methylation sequencing to detect targeted methylation regions, and, based on the methylation window features obtained from prior bioinformatics analysis, screens age-related features in the training set samples to determine the core methylation window features used for modeling. Furthermore, it constructs a stable and interpretable regularized linear age prediction model based on elastic network regression. This invention has advantages such as non-invasive sampling, low cost, rapid detection, accurate results, and interpretable model, enabling efficient, accurate, and standardized assessment of canine biological age. It is suitable for pet health management, aging evaluation, early warning of geriatric diseases, and monitoring of anti-aging intervention effects.