47 results about "Epigenetics" patented technology

The invention discloses application of a gene marker in early screening of esophagus, stomach and intestine multiple cancer species, an early screening model construction method and a detection device, and belongs to the technical field of early noninvasive detection of digestive tract tumors. By analyzing the whole genome characteristics of circulating free DNA in peripheral blood, a novel multi-cancer-species screening system is established. On the basis of low-depth whole genome sequencing data, molecular markers in three dimensions, namely a genome copy number variation mode, a DNA fragment distribution characteristic with a specific length and an epigenetics signal of a transcription initiation region, are emphatically detected. An advanced converter neural network architecture is adopted, and the model can efficiently capture complex feature association in a whole genome range through a specific self-attention mechanism. The model design particularly considers the particularity of genome data, introduces an adaptive position coding system, and accurately reflects the spatial distribution relationship of DNA fragments on chromosomes. Therefore, the system can still maintain excellent detection performance under extremely low sequencing depth.

The invention belongs to the technical field of bioinformatics, and discloses an epigenetics age evaluation system and method based on machine learning. Comprising the following steps: collecting methylation data of a user in real time; screening out methylated feature data from the methylated data, and compressing the methylated feature data into hidden feature data; obtaining historical feature data, and constructing an age evaluation model; inputting hidden feature data acquired in real time into the age evaluation model, predicting an epigenetic age, and marking the epigenetic age as a predicted age; systematic disturbance is carried out on the predicted age, the predicted error value of each simulated age is calculated, and the simulated age corresponding to the predicted error value with the minimum numerical value is used as a corrected age; calculating a relative age, and taking a mean value of the relative age and the corrected age as an epigenetics age of the user; according to the method, the adaptability and accuracy of the model on different individuals and age groups can be improved, so that the accuracy and timeliness of epigenetics age assessment are improved.

The technology disclosed relates to reliably identifying variants that cause extreme levels of gene expression. Extreme levels of gene expression include under expression and over expression. Then, these variants are used to train artificial intelligence based models for a variety of prediction tasks. One example of the prediction tasks is to produce per-base resolution for chromatin sequences. Another example of the chromatin task is to produce gene expression changes caused by the reliably identified variants.

The invention relates to the technical field of cosmetics, and discloses a composition and application thereof to preparation of a reagent for intervening lip aging. The composition comprises Inca inchi seed oil, stearyl glycyrrhizinate and a myrothamnus flabellifolia leaf and stem extract, wherein the mass ratio of the Inca inchi seed oil to the stearyl glycyrrhizinate to the myrothamnus flabellifolia leaf and stem extract is (1-15): 1: (0.2-10). According to the composition, through scientific compounding and a multi-target epigenetics mechanism, the function of tough lip blood vessels is improved and maintained, meanwhile, the epidermis is repaired, and lip rejuvenation is achieved through the dual effects of the epidermis and the blood vessels.

The invention discloses a whole genome methylation detection method based on MeDIP-MSRE, and belongs to the technical field of epigenetics detection. According to the method, methylation immunoprecipitation sequencing and a methylation sensitive restriction enzyme technology are innovatively combined, firstly, a methylation specific antibody is used for conducting immunoprecipitation on sample DNA, and whole genome methylation fragments are enriched; then carrying out enzyme digestion on the enriched product by adopting methylation sensitive restriction enzyme, specifically removing an unmethylated DNA region, and reserving a complete methylation sequence; and finally, constructing a methylation map through high-throughput sequencing. According to the method, traditional hydrosulfite chemical conversion is not needed, DNA damage and base conversion deviation caused by the traditional hydrosulfite chemical conversion are avoided, meanwhile, high sensitivity of MeDIP and high specificity of methylation sensitive restriction endonuclease are fused, and the fidelity, sensitivity and specificity of detection are remarkably improved.

Described herein are methods and compositions related to methylation-based determination and characterization of subjects afflicted with various stages of cancer, including lung cancer and non-small cell lung cancer (NSCLC). Osimertinib is one of the most common precision therapies in non-small cell lung cancer but nearly all patients eventually progress due to somatic mutations and cellular plasticity that bypass the treatment. Defining the cellular plasticity state with epigenetics will help physicians know when to switch therapies.

The invention discloses an epigenetics method for improving the cryopreservation efficiency of sheep semen. The method comprises the following steps: extracting seminal fluid of Donflilien and Hu sheep hybrid F1-generation sheep, dividing the seminal fluid into a fresh group and a frozen group, carrying out somatic cell removal and small non-coding RNA extraction, screening out differentially expressed microRNAs by utilizing a Pandorah sequencing technology, analyzing a target gene and a signal channel of the microRNAs, and determining the seminal fluid of the Donflilien and Hu sheep hybrid F1-generation sheep. The small non-coding RNA related to sperm cryopreservation is found to mainly relate to key biological processes such as oxidative stress response and cell surface receptor signal channels. The screened differentially expressed small non-coding RNA is added into the frozen semen through methods such as in vitro chemical synthesis, so that the artificial fertilization conception rate of the frozen semen is remarkably increased.

The application belongs to the technical field of bioinformatics, and discloses an epigenetic age assessment system and method based on machine learning; comprising: collecting methylation data of a user in real time; screening methylation feature data from the methylation data and compressing the methylation feature data into hidden feature data; obtaining historical feature data and constructing an age assessment model; inputting the hidden feature data obtained in real time into the age assessment model, predicting an epigenetic age, and marking the epigenetic age as a predicted age; systematically perturbing the predicted age, calculating a prediction error value of each simulated age, taking a simulated age corresponding to a minimum prediction error value as a corrected age; calculating a relative age, and taking a mean value of the relative age and the corrected age as an epigenetic age of the user; the application can improve the adaptability and accuracy of the model on different individuals and age groups, thereby improving the accuracy and timeliness of epigenetic age assessment.

The application discloses a whole-genome DNA cytosine hydroxymethylation modification single-base precision library construction method, relates to the technical field of genomics, epigenetics and molecular biology, and contains the following steps: 5hmC labeling reaction, click chemistry reaction, sample DNA fragmentation, purification of the fragmentation product through magnetic beads, 5hmC fragmented DNA capture, washing of the capture product, reduction reaction, APOBEC enzyme deamination reaction, purification of the deamination product, double-strand conversion reaction, end repair and 3' end A addition reaction, linker ligation, purification of the ligation product and double sorting, library amplification and library purification. The library construction method integrates low data volume, single-base precision and conventional double-strand library construction kit in one under the premise of ensuring data authenticity, and has the technical advantages of high universality and highly repeatable data.

The application discloses a kind of gene markers in esophagus, stomach, intestine multiple cancer early screening application, early screening model construction method and detection device, belong to the early non-invasive detection technical field of digestive tract tumor.It establishes a new type of multiple cancer screening system by analyzing the whole genome characteristics of circulating free DNA in peripheral blood.Based on low-depth whole genome sequencing data, three dimensions of molecular markers are detected: genome copy number variation pattern, DNA fragment distribution characteristics of specific length and epigenetic signals of transcription initiation region.Advanced converter neural network architecture is used, and the model can efficiently capture the complex feature correlation in the whole genome range through its unique self-attention mechanism.The model design specially considers the particularity of genomic data, and introduces an adaptive position coding system to accurately reflect the spatial distribution relationship of DNA fragments on the chromosome.The system can still maintain excellent detection performance at very low sequencing depth.

This invention provides an application of a specific DNA molecule in identifying the authentic quality of Dendrobium officinale. The specific DNA molecule includes the sequence shown in SEQ ID NO.1. Based on the principles of epigenetics, this invention identifies environment-associated molecular markers in Dendrobium officinale grown under different environments. Using these markers for quantitative real-time PCR, the growth environment of Dendrobium officinale materials can be distinguished within one day. The procedure is simplified, easy to operate, and cost-effective, providing an objective and qualitative detection method for the authentic quality of Dendrobium officinale.

The invention discloses application of a PLAC8 promoter methylation promoter in preparation of a product for promoting angiogenesis of a pig placenta, and belongs to the technical field of biological medicines. Experiments prove that PLAC8 as an anti-angiogenesis factor can directly inhibit the angiogenesis function, and DNMT3A can promote placenta vascular development and improve sow farrowing performance by regulating the methylation level of a PLAC8 promoter. In addition, it is determined that daily ration supplementation of methyl donors in the pig placenta vascular development peak period is an important nutrition way for improving DNA methylation of the PLAC8 promoter region. According to the method, the placenta injury mechanism under the adverse pregnancy outcome is directly determined through combination of in-vivo and in-vitro experiments, a theoretical basis is provided for analysis of a regulation strategy for promoting pig placenta angiogenesis and improving sow reproductive performance from epigenetics, and the accuracy and credibility of a result are improved.

This invention relates to the fields of traditional Chinese medicine and anti-aging technology. The invention provides a scientifically formulated traditional Chinese medicine composition based on epigenetic guidance, its preparation method, and its application. The composition is made from the following raw materials in parts by weight: ginseng 3-12 parts, cordyceps 3-12 parts, polygonatum 2-8 parts, ganoderma lucidum 2-10 parts, mulberry fungus 2-6 parts, pine needles 1-6 parts, epimedium 2-8 parts, salvia miltiorrhiza 3-10 parts, bletilla striata 1-5 parts, cistanche deserticola 2-6 parts, rosemary 1-5 parts, and banana peel 1-6 parts. The composition is designed to comprehensively consider aging-related oxidative stress, energy metabolism regulation, and multi-component intervention needs, and is constructed in conjunction with epigenetic regulatory directions, reflecting the standardized design concept of scientifically formulated traditional Chinese medicine. The composition of this invention has the characteristics of clear composition, feasible preparation process, controllable quality parameters, and verifiable efficacy indicators.

The application provides a preparation method of a cell membrane coated nanometer drug delivery platform and belongs to the technical field of drug delivery systems. The prepared NH2-MIL-88B(Fe)-MG132 material is used as a drug carrier to construct a cell membrane coated nanometer drug delivery platform, so that the targeted chemical dynamic therapy and the epigenetic regulation combined treatment effect are achieved. The drug delivery platform constructed by the application has the advantages of high targeting and low toxic side effects, reduces the phagocytosis of macrophages before the concentration in the tumor microenvironment, plays a certain role in activating the body and tumor related immune activity, and even achieves the effect of immunogenic site death of tumor cells. The drug delivery platform has high targeting and specificity, no drug burst release, high water solution stability, and higher aggregation in the tumor site.

The invention discloses a gene for regulating skeletal muscle differentiation and muscle fiber type conversion and application thereof, and relates to the technical field of biology. The nucleotide sequence of the gene is as shown in SEQ ID NO. 1. Experiments prove that the MSS51 gene promotes skeletal muscle differentiation and accelerates conversion from rapid glycolytic muscle fibers to slow oxidation muscle fibers. In addition, the YTHDF2 and the IGF2BP2 are remarkably and highly expressed in the solefish muscle tissue. Therefore, YTHDF2 or IGF2BP2 is supposed to be combined with the m6A peak of the MSS513 '-UTR region in a targeted manner and promote the stability of MSS51, and finally the differentiation and muscle fiber transformation of skeletal muscles are regulated and controlled. The invention provides a new insight for gene epigenetics in muscle fiber type conversion, and preliminarily reveals the meat yield and the genetic and regulation mechanism of the meat quality.

The invention belongs to the field of daily chemicals, and discloses a gene-level anti-aging composition for inhibiting DNA methylation, which comprises the following components in percentage by weight: 50-70% of hydrolyzed royal jelly protein complex; 30 to 50 percent of panthenol; and 1-3% of a kelp extract. The composition takes a hydrolyzed royal jelly protein compound, panthenol and a kelp extract as main active ingredients, the composition is proved to have an excellent apparent anti-aging function through an epigenetics anti-aging test, and an excellent anti-aging effect can be achieved under the condition that the hydrolyzed royal jelly protein compound, the panthenol and the kelp extract are relatively small in dosage through related anti-aging tests. Meanwhile, the invention further discloses application of the composition and cosmetics.

To provide methods of treating WHSC1-overexpressing cancers by inhibiting SETD2.SOLUTION: The present disclosure relates to epigenetic-based cancer therapy, and the unexpected discovery that inhibiting SETD2, despite its functionality as a tumor suppressor, can be used to treat cancers. Additionally, the present disclosure relates to the unexpected discovery that inhibiting SETD2 can be used to treat cancers that overexpress WHSC1.SELECTED DRAWING: None

The invention relates to an immunofluorescence method and kit for detecting zebra fish heart DNA methylation level, and belongs to the technical field of biological detection. The immunofluorescence method provided by the invention comprises the following steps: exposing a zebrafish embryo to a substance to be detected, dissecting a heart sample of the zebrafish embryo and carrying out fixation, membrane permeation, protease K digestion, hydrochloric acid DNA denaturation and neutralization treatment, and then carrying out sealing, incubation of a 5-methylcytosine primary antibody and a fluorescently labeled secondary antibody, cell nucleus staining and fluorescence detection. And finally, analyzing and evaluating the DNA methylation level through fluorescence imaging. According to the method disclosed by the invention, through a combined step of protease K and hydrochloric acid denaturation treatment, the binding efficiency to the antibody is remarkably improved, so that high-sensitivity and low-background detection on the methylation level of the whole DNA in a zebra fish micro heart sample is realized; the detection kit provided by the invention can be widely applied to epigenetics evaluation of toxicity of environmental pollutants.

Disclosed herein is a novel DNA methylation-based tool designed to predict intrinsic capacity, which encompasses the sum of an individual's physical and mental capacities. A computational model was developed that, given the DNA methylation levels of 91 Cytosine-phosphate-Guanine (CpGs) sites in the blood, calculates an estimate of intrinsic capacity.

The present invention discloses a lysate for animal tissue and a method for using the same in CUT&Tag. The present invention relates to the fields of biotechnology and epigenetic technology, and specifically provides a lysate suitable for animal tissue and a method for using the same in CUT&Tag. The lysate contains an appropriate amount of mild non-ionic detergent NP40 (or CA630) and Triton X-100, which can efficiently lyse tissue clumps while ensuring the integrity of cell nuclei, promote the rapid release of cell nuclei, and thereby obtain cell nuclei suitable for the construction of tissue CUT&Tag libraries. This makes it possible to implement CUT&Tag technology at the animal tissue level, and has great application advantages and high commercial value in the field of epigenetic technology research.

The application discloses a data analysis method in the field of epigenetics: a quantitative analysis method of RNA 5-methylcytosine modification on splicing isomers. The method can solve the problem of how to quantitatively analyze RNA 5-methylcytosine modification on the level of gene splicing isomers, and the technical points include: converting the data set generated by the RNA-BSseq sequencing technology into a virtual RNA-Seq data set and marking the data in which RNA 5-methylcytosine modification occurs; estimating the expression amount of the splicing isomer in the virtual data set, and calculating the mean and variance thereof by using the bootstrap technology; estimating the RNA 5-methylcytosine modification level of the splicing isomer, and calculating the mean and variance of the RNA 5-methylcytosine modification level of the splicing isomer by using the Delta technology; and based on the normal distribution, testing whether there is a significant difference in the estimated RNA 5-methylcytosine modification level of the splicing isomer under different conditions. The application helps to reveal the regulation mechanism of RNA 5-methylcytosine modification in physiological processes and pathological changes.

The application discloses a solution for separating fat cell nucleus and cytoplasm, and the solution comprises solution 1 and solution 2; wherein the solution 1 comprises 8-12 mM hydrochloride buffer, 0.03-0.15% Tween-20, 8-12 mM sodium chloride, 2-4 mM magnesium chloride, 0.8-2% bovine serum albumin and 0.4-0.6% ethyl phenyl polyethylene glycol; the solution 2 comprises 8-12 mM hydrochloride buffer, 8-25 mM sodium chloride, 1-15 mM magnesium chloride and 0.8-2.5% bovine serum albumin; the percentage is volume percentage. The application can realize the research on all cell types of fat tissues, greatly reduces the required amount of fat tissues and improves the possibility of carrying out single cell research. The application can be used for both transcriptome research and epigenetic research.

The purpose of the present invention is to provide molecules that induce, in cells, the decomposition of proteins associated with epigenetic modification, particularly enzymes (e.g. DNA methyltransferase) that catalyze methylation of nucleic acids.　More specifically, the purpose of the present invention is to provide a compound represented by formula (I) or a salt thereof, or a solvate or a hydrate of the same. [In formula (I), A is a double-stranded nucleic acid and includes, in one strand, at least one 5'-CpG-3' sequence in which the 5-position of cytosine (C) has been methylated, the C of the 3'-GpC-5' that is complementary to said 5'-CpG-3' may be a modified base for covalently bonding DNMT1 to A, L is a hydrocarbon that may include oxygen, sulfur, phosphorus, or nitrogen, and B is a ligand molecule of an E3 ubiquitin ligase.]

To provide a defined culture medium for isolating stratified epithelial stem cells and stably maintaining the epigenetics of the stratified epithelial stem cells during multiple passages, and to provide clonal stratified epithelial stem cells isolated using the defined culture medium.SOLUTION: And a defined culture medium comprising each of ROCK (Rho kinase) inhibitors, mitogenic growth factors, insulin or IGF, TrkA inhibitors, and Oct4 activators, and comprising at least one of VEGF inhibitors, tyrosine kinase inhibitors, and / or FGF10 or FGF10 agonists, and optionally further comprising TGF β signaling pathway inhibitors and / or bone morphogenetic proteins (BMP) antagonists, wherein the defined culture medium supports epigenetically stable growth and proliferation of stem cells of stratified epithelial tissue origin in culture.SELECTED DRAWING: Figure 1

The invention relates to the cross technical field of bioinformatics and epigenetics, and particularly discloses a method for rapidly identifying plant imprinting genes based on multi-species sequence conservation.The method comprises the steps that a whole genome gene set of target plant species is obtained; selecting reference plant species with different genetic relationships with the target plant species, obtaining an optimal homologous gene of each gene of the target plant species in the reference plant species, and constructing a multi-species homologous sequence set; performing multi-sequence comparison on the multi-species homologous sequence set, and calculating a conservative score of each target gene based on a comparison result; and sorting and screening the target genes based on the conservative scores, and preliminarily identifying the genes with the conservative scores higher than a set threshold as candidate plant imprinting genes. According to the method, high-throughput and low-cost screening and prediction of potential plant imprinting genes are realized by utilizing the characteristic that the plant imprinting genes have higher conservative property in an evolution process and calculating cross-species conservative property scores of the genes.

The invention provides herein epigenetics changes identified in differentially methylated regions (DMRs) of DNA of a father that are associated with the risk for an offspring having autism spectrum disorder (ASD). The invention further provides methods of determining a risk of having an offspring with ASD, methods of diagnosing ASD in a subject, and methods of determining an association between exposure to an environmental factor in a subject and an increased risk of having an offspring with ASD. The invention also provides a kit for determining whether a subject has or is at risk of having or inheriting a risk of having ASD.

The present application provides a Saccharomyces cerevisiae histone H3K18Q point mutation, a point mutant and an application, which belongs to the field of genetic engineering technology. The present application obtains a Saccharomyces cerevisiae histone H3K18Q point mutation by mutating the 18th lysine at the N-terminus of the Saccharomyces cerevisiae histone, which can be acetylated, into glutamine, and transfers the pJFE3‑XI plasmid into the Saccharomyces cerevisiae histone H3K18Q point mutation to form a Saccharomyces cerevisiae histone H3K18Q point mutant. The Saccharomyces cerevisiae histone H3K18Q point mutant can significantly increase the xylose consumption rate and ethanol yield of recombinant Saccharomyces cerevisiae, and can be directly used in the performance optimization of lignocellulosic ethanol production strains, providing a reference for the application of epigenetics in second-generation ethanol production, and providing a new theoretical basis and technical route for strain optimization in the industrial production of lignocellulosic ethanol.

The invention relates to the field of bioengineering technology and biomedicine, and discloses a nucleic acid modified protein and application thereof. The TdpC protein disclosed by the invention not only shows efficient catalytic activity in DNA phosphorus-sulfur acylation modification and specific adenylation of a DNA phosphate skeleton, but also can stably work in a high-temperature, strong-acidity or high-sulfur-enrichment extreme environment, so that the TdpC protein has wide application potential in the extreme environment. Experimental results fully prove that the method has high efficiency and applicability in the fields related to epigenetics research, genomics research and biological medicine.

The invention provides a biological microsphere and a preparation method thereof, and a multi-skin appendage in-situ regeneration method, the biological microsphere is constructed by loading a small molecule compound combination and a protein by using a lipid carrier sphere, the small molecule compound combination and the protein are integrated, and the lipid carrier sphere is used for slow release, so that epigenetics can be deeply changed; the method has the advantages of maintaining cell proliferation, activating hair papilla cell and sweat gland cell fate, greatly promoting the cell fate conversion efficiency, achieving cell reprogramming, being safe, efficient and easy to operate, and avoiding the safety risk of gene editing, thereby effectively solving the problems of wound repairing, scar weakening and skin functional regeneration.

The invention discloses a crowd specific imprinting region detection method based on a long-read-long sequencing technology, and relates to the fields of bioinformatics and epigenetics, in particular to the crowd specific imprinting region detection method based on the long-read-long sequencing technology. The problems that existing hDMR recognition precision is low, a statistical model is too simple, and a standardized process is lacked are solved. The method comprises the following steps of: 1, acquiring a sequenced BAM file with methylation information of each person in a group of people; 2, performing haplotype typing on the BAM file to obtain a hap1 file and a hap2 file after typing; 3, performing haplotype differential methylation region identification on the hap1 file and the hap2 file to obtain a candidate mark region of the person 1; 4, obtaining a candidate mark area of one crowd; 5, repeatedly executing the steps 1 to 4 to obtain a candidate mark area of another group of crowds; and 6, based on the candidate imprint areas of the two crowds, obtaining specific imprint areas of the two crowds.