69 results about "Epigenetics" patented technology

The invention discloses application of a gene marker in early screening of esophagus, stomach and intestine multiple cancer species, an early screening model construction method and a detection device, and belongs to the technical field of early noninvasive detection of digestive tract tumors. By analyzing the whole genome characteristics of circulating free DNA in peripheral blood, a novel multi-cancer-species screening system is established. On the basis of low-depth whole genome sequencing data, molecular markers in three dimensions, namely a genome copy number variation mode, a DNA fragment distribution characteristic with a specific length and an epigenetics signal of a transcription initiation region, are emphatically detected. An advanced converter neural network architecture is adopted, and the model can efficiently capture complex feature association in a whole genome range through a specific self-attention mechanism. The model design particularly considers the particularity of genome data, introduces an adaptive position coding system, and accurately reflects the spatial distribution relationship of DNA fragments on chromosomes. Therefore, the system can still maintain excellent detection performance under extremely low sequencing depth.

The invention belongs to the technical field of bioinformatics, and discloses an epigenetics age evaluation system and method based on machine learning. Comprising the following steps: collecting methylation data of a user in real time; screening out methylated feature data from the methylated data, and compressing the methylated feature data into hidden feature data; obtaining historical feature data, and constructing an age evaluation model; inputting hidden feature data acquired in real time into the age evaluation model, predicting an epigenetic age, and marking the epigenetic age as a predicted age; systematic disturbance is carried out on the predicted age, the predicted error value of each simulated age is calculated, and the simulated age corresponding to the predicted error value with the minimum numerical value is used as a corrected age; calculating a relative age, and taking a mean value of the relative age and the corrected age as an epigenetics age of the user; according to the method, the adaptability and accuracy of the model on different individuals and age groups can be improved, so that the accuracy and timeliness of epigenetics age assessment are improved.

The invention discloses application of miRNA-142a-5p in preparation of a biological medicine for intervening or treating retinitis pigmentosa, and aims to solve the technical problem that retinitis pigmentosa lesion is difficult to intervene and treat. The research finds that miRNA-142a-5p directly participates in the process of retinitis pigmentosa of rd1 mice, the pathological process of the retinitis pigmentosa can be obviously delayed by down-regulating the expression of the miRNA-142a-5p, and an epigenetics potential precise treatment target is provided for reversing the retinitis pigmentosa; the scAAV virus vector for knocking down the miRNA-142a-5p is further constructed and is used for subretinal space injection, and the retinitis pigmentosa degeneration process can be obviously delayed.

The invention discloses a skin care product anti-aging effect multi-dimensional comprehensive detection system, which comprises an in-vitro cell model experiment module for detecting indexes such as cell activity, proliferation, migration, apoptosis and antioxidant enzyme activity by simulating natural aging and light aging conditions; the content change of skin structure components such as collagen, elastic fibers and laminin is quantitatively analyzed, the DNA damage repair capacity is reflected by measuring the expression quantity of gammaH2AX, epigenetics research is developed, and the methylation state change of a CpG island is monitored; and the tissue engineering and three-dimensional skin model experiment module is used for performing morphological observation, biochemical index detection and skin physiological parameter evaluation under the condition of simulating a real skin environment by utilizing an artificial skin model. The whole process from the in-vitro cell model to the clinical human body test is covered, and the actual efficiency of the skin care product for delaying or improving the skin aging process can be accurately and objectively evaluated through multi-angle and multi-level data integration analysis.

The technology disclosed relates to reliably identifying variants that cause extreme levels of gene expression. Extreme levels of gene expression include under expression and over expression. Then, these variants are used to train artificial intelligence based models for a variety of prediction tasks. One example of the prediction tasks is to produce per-base resolution for chromatin sequences. Another example of the chromatin task is to produce gene expression changes caused by the reliably identified variants.

The invention relates to the technical field of cosmetics, and discloses a composition and application thereof to preparation of a reagent for intervening lip aging. The composition comprises Inca inchi seed oil, stearyl glycyrrhizinate and a myrothamnus flabellifolia leaf and stem extract, wherein the mass ratio of the Inca inchi seed oil to the stearyl glycyrrhizinate to the myrothamnus flabellifolia leaf and stem extract is (1-15): 1: (0.2-10). According to the composition, through scientific compounding and a multi-target epigenetics mechanism, the function of tough lip blood vessels is improved and maintained, meanwhile, the epidermis is repaired, and lip rejuvenation is achieved through the dual effects of the epidermis and the blood vessels.

The invention discloses a whole genome methylation detection method based on MeDIP-MSRE, and belongs to the technical field of epigenetics detection. According to the method, methylation immunoprecipitation sequencing and a methylation sensitive restriction enzyme technology are innovatively combined, firstly, a methylation specific antibody is used for conducting immunoprecipitation on sample DNA, and whole genome methylation fragments are enriched; then carrying out enzyme digestion on the enriched product by adopting methylation sensitive restriction enzyme, specifically removing an unmethylated DNA region, and reserving a complete methylation sequence; and finally, constructing a methylation map through high-throughput sequencing. According to the method, traditional hydrosulfite chemical conversion is not needed, DNA damage and base conversion deviation caused by the traditional hydrosulfite chemical conversion are avoided, meanwhile, high sensitivity of MeDIP and high specificity of methylation sensitive restriction endonuclease are fused, and the fidelity, sensitivity and specificity of detection are remarkably improved.

The present invention relates to a method of determining whether a test animal and / or a product-obtaining test animal has received and / or is currently receiving treatment with at least one antibiotic and / or veterinary chemical, comprising: (a) determining a test methylation profile from genomic material, the genomic material is contained in a biological sample obtained from a test animal and / or an animal-derived product; and (b) comparing the test methylation profile obtained from (a) to a reference methylation profile obtained from a control animal of the same biometric population of the test animal wherein the control animal has not been accepted and / or has not currently accepted treatment with at least one antibiotic and / or veterinary chemical, wherein a difference in the test methylation profile of (a) compared to a reference methylation profile from a control animal indicates that the test animal has accepted and / or is currently accepted treatment with at least one antibiotic and / or veterinary chemical; and wherein the test animal is a terrestrial animal, and wherein the veterinary chemical is an antiparasitic agent, an antiviral agent, a feed additive, a water additive, a disinfectant, glutaraldehyde and / or formalin.

The invention discloses a fusion protein based on double-stranded DNA deaminase and an epigenetic modification detection method. The fusion protein comprises a molecule having a specific affinity for an antibody, a linker peptide, and a double-stranded DNA deaminase. Double-stranded DNA deaminase is guided to a specific antibody through a molecule with specific affinity to the antibody, so that a deamination reaction is performed on a DNA sequence of a target site of the antibody, and a chromatin-associated protein targeted by the antibody or the position of the chromatin-associated protein modified on the double-stranded DNA sequence is detected according to the determined position of the DNA deamination site.

Described herein are methods and compositions related to methylation-based determination and characterization of subjects afflicted with various stages of cancer, including lung cancer and non-small cell lung cancer (NSCLC). Osimertinib is one of the most common precision therapies in non-small cell lung cancer but nearly all patients eventually progress due to somatic mutations and cellular plasticity that bypass the treatment. Defining the cellular plasticity state with epigenetics will help physicians know when to switch therapies.

The invention discloses an epigenetics method for improving the cryopreservation efficiency of sheep semen. The method comprises the following steps: extracting seminal fluid of Donflilien and Hu sheep hybrid F1-generation sheep, dividing the seminal fluid into a fresh group and a frozen group, carrying out somatic cell removal and small non-coding RNA extraction, screening out differentially expressed microRNAs by utilizing a Pandorah sequencing technology, analyzing a target gene and a signal channel of the microRNAs, and determining the seminal fluid of the Donflilien and Hu sheep hybrid F1-generation sheep. The small non-coding RNA related to sperm cryopreservation is found to mainly relate to key biological processes such as oxidative stress response and cell surface receptor signal channels. The screened differentially expressed small non-coding RNA is added into the frozen semen through methods such as in vitro chemical synthesis, so that the artificial fertilization conception rate of the frozen semen is remarkably increased.

The invention discloses a biosensor for simultaneously detecting N6-methyladenosine at a single molecule level and a preparation method and application of the biosensor, and the preparation method of the biosensor comprises the following steps: step 1, incubating a reaction solution containing mRNA, a MazF buffer solution and MazF to complete a MazF cleavage reaction; and 2, adding a reaction product in the step 1 into a reaction system containing the hairpin probe, a DSN buffer solution and DSN, and incubating to complete DSN digestion reaction. The biosensor provided by the invention can be used for simultaneously detecting two m6A sites in different mRNAs with single nucleotide resolution. Besides, the method can identify 0.01% RNA methylation level, can distinguish m6A level between cancer cells / tissues and normal cells / tissues, and has a great application prospect in cancer detection and RNA epigenetics research.

The application belongs to the technical field of bioinformatics, and discloses an epigenetic age assessment system and method based on machine learning; comprising: collecting methylation data of a user in real time; screening methylation feature data from the methylation data and compressing the methylation feature data into hidden feature data; obtaining historical feature data and constructing an age assessment model; inputting the hidden feature data obtained in real time into the age assessment model, predicting an epigenetic age, and marking the epigenetic age as a predicted age; systematically perturbing the predicted age, calculating a prediction error value of each simulated age, taking a simulated age corresponding to a minimum prediction error value as a corrected age; calculating a relative age, and taking a mean value of the relative age and the corrected age as an epigenetic age of the user; the application can improve the adaptability and accuracy of the model on different individuals and age groups, thereby improving the accuracy and timeliness of epigenetic age assessment.

The application discloses a whole-genome DNA cytosine hydroxymethylation modification single-base precision library construction method, relates to the technical field of genomics, epigenetics and molecular biology, and contains the following steps: 5hmC labeling reaction, click chemistry reaction, sample DNA fragmentation, purification of the fragmentation product through magnetic beads, 5hmC fragmented DNA capture, washing of the capture product, reduction reaction, APOBEC enzyme deamination reaction, purification of the deamination product, double-strand conversion reaction, end repair and 3' end A addition reaction, linker ligation, purification of the ligation product and double sorting, library amplification and library purification. The library construction method integrates low data volume, single-base precision and conventional double-strand library construction kit in one under the premise of ensuring data authenticity, and has the technical advantages of high universality and highly repeatable data.

The application discloses a kind of gene markers in esophagus, stomach, intestine multiple cancer early screening application, early screening model construction method and detection device, belong to the early non-invasive detection technical field of digestive tract tumor.It establishes a new type of multiple cancer screening system by analyzing the whole genome characteristics of circulating free DNA in peripheral blood.Based on low-depth whole genome sequencing data, three dimensions of molecular markers are detected: genome copy number variation pattern, DNA fragment distribution characteristics of specific length and epigenetic signals of transcription initiation region.Advanced converter neural network architecture is used, and the model can efficiently capture the complex feature correlation in the whole genome range through its unique self-attention mechanism.The model design specially considers the particularity of genomic data, and introduces an adaptive position coding system to accurately reflect the spatial distribution relationship of DNA fragments on the chromosome.The system can still maintain excellent detection performance at very low sequencing depth.

This invention provides an application of a specific DNA molecule in identifying the authentic quality of Dendrobium officinale. The specific DNA molecule includes the sequence shown in SEQ ID NO.1. Based on the principles of epigenetics, this invention identifies environment-associated molecular markers in Dendrobium officinale grown under different environments. Using these markers for quantitative real-time PCR, the growth environment of Dendrobium officinale materials can be distinguished within one day. The procedure is simplified, easy to operate, and cost-effective, providing an objective and qualitative detection method for the authentic quality of Dendrobium officinale.

The invention discloses application of a PLAC8 promoter methylation promoter in preparation of a product for promoting angiogenesis of a pig placenta, and belongs to the technical field of biological medicines. Experiments prove that PLAC8 as an anti-angiogenesis factor can directly inhibit the angiogenesis function, and DNMT3A can promote placenta vascular development and improve sow farrowing performance by regulating the methylation level of a PLAC8 promoter. In addition, it is determined that daily ration supplementation of methyl donors in the pig placenta vascular development peak period is an important nutrition way for improving DNA methylation of the PLAC8 promoter region. According to the method, the placenta injury mechanism under the adverse pregnancy outcome is directly determined through combination of in-vivo and in-vitro experiments, a theoretical basis is provided for analysis of a regulation strategy for promoting pig placenta angiogenesis and improving sow reproductive performance from epigenetics, and the accuracy and credibility of a result are improved.

This invention relates to the fields of traditional Chinese medicine and anti-aging technology. The invention provides a scientifically formulated traditional Chinese medicine composition based on epigenetic guidance, its preparation method, and its application. The composition is made from the following raw materials in parts by weight: ginseng 3-12 parts, cordyceps 3-12 parts, polygonatum 2-8 parts, ganoderma lucidum 2-10 parts, mulberry fungus 2-6 parts, pine needles 1-6 parts, epimedium 2-8 parts, salvia miltiorrhiza 3-10 parts, bletilla striata 1-5 parts, cistanche deserticola 2-6 parts, rosemary 1-5 parts, and banana peel 1-6 parts. The composition is designed to comprehensively consider aging-related oxidative stress, energy metabolism regulation, and multi-component intervention needs, and is constructed in conjunction with epigenetic regulatory directions, reflecting the standardized design concept of scientifically formulated traditional Chinese medicine. The composition of this invention has the characteristics of clear composition, feasible preparation process, controllable quality parameters, and verifiable efficacy indicators.

The application provides a preparation method of a cell membrane coated nanometer drug delivery platform and belongs to the technical field of drug delivery systems. The prepared NH2-MIL-88B(Fe)-MG132 material is used as a drug carrier to construct a cell membrane coated nanometer drug delivery platform, so that the targeted chemical dynamic therapy and the epigenetic regulation combined treatment effect are achieved. The drug delivery platform constructed by the application has the advantages of high targeting and low toxic side effects, reduces the phagocytosis of macrophages before the concentration in the tumor microenvironment, plays a certain role in activating the body and tumor related immune activity, and even achieves the effect of immunogenic site death of tumor cells. The drug delivery platform has high targeting and specificity, no drug burst release, high water solution stability, and higher aggregation in the tumor site.

The invention discloses a gene for regulating skeletal muscle differentiation and muscle fiber type conversion and application thereof, and relates to the technical field of biology. The nucleotide sequence of the gene is as shown in SEQ ID NO. 1. Experiments prove that the MSS51 gene promotes skeletal muscle differentiation and accelerates conversion from rapid glycolytic muscle fibers to slow oxidation muscle fibers. In addition, the YTHDF2 and the IGF2BP2 are remarkably and highly expressed in the solefish muscle tissue. Therefore, YTHDF2 or IGF2BP2 is supposed to be combined with the m6A peak of the MSS513 '-UTR region in a targeted manner and promote the stability of MSS51, and finally the differentiation and muscle fiber transformation of skeletal muscles are regulated and controlled. The invention provides a new insight for gene epigenetics in muscle fiber type conversion, and preliminarily reveals the meat yield and the genetic and regulation mechanism of the meat quality.

The invention belongs to the field of daily chemicals, and discloses a gene-level anti-aging composition for inhibiting DNA methylation, which comprises the following components in percentage by weight: 50-70% of hydrolyzed royal jelly protein complex; 30 to 50 percent of panthenol; and 1-3% of a kelp extract. The composition takes a hydrolyzed royal jelly protein compound, panthenol and a kelp extract as main active ingredients, the composition is proved to have an excellent apparent anti-aging function through an epigenetics anti-aging test, and an excellent anti-aging effect can be achieved under the condition that the hydrolyzed royal jelly protein compound, the panthenol and the kelp extract are relatively small in dosage through related anti-aging tests. Meanwhile, the invention further discloses application of the composition and cosmetics.

To provide methods of treating WHSC1-overexpressing cancers by inhibiting SETD2.SOLUTION: The present disclosure relates to epigenetic-based cancer therapy, and the unexpected discovery that inhibiting SETD2, despite its functionality as a tumor suppressor, can be used to treat cancers. Additionally, the present disclosure relates to the unexpected discovery that inhibiting SETD2 can be used to treat cancers that overexpress WHSC1.SELECTED DRAWING: None

The invention relates to an immunofluorescence method and kit for detecting zebra fish heart DNA methylation level, and belongs to the technical field of biological detection. The immunofluorescence method provided by the invention comprises the following steps: exposing a zebrafish embryo to a substance to be detected, dissecting a heart sample of the zebrafish embryo and carrying out fixation, membrane permeation, protease K digestion, hydrochloric acid DNA denaturation and neutralization treatment, and then carrying out sealing, incubation of a 5-methylcytosine primary antibody and a fluorescently labeled secondary antibody, cell nucleus staining and fluorescence detection. And finally, analyzing and evaluating the DNA methylation level through fluorescence imaging. According to the method disclosed by the invention, through a combined step of protease K and hydrochloric acid denaturation treatment, the binding efficiency to the antibody is remarkably improved, so that high-sensitivity and low-background detection on the methylation level of the whole DNA in a zebra fish micro heart sample is realized; the detection kit provided by the invention can be widely applied to epigenetics evaluation of toxicity of environmental pollutants.

Disclosed herein is a novel DNA methylation-based tool designed to predict intrinsic capacity, which encompasses the sum of an individual's physical and mental capacities. A computational model was developed that, given the DNA methylation levels of 91 Cytosine-phosphate-Guanine (CpGs) sites in the blood, calculates an estimate of intrinsic capacity.

The invention belongs to the technical field of microbial preparations, and particularly relates to a metagen for synergistically improving the hypoglycemic capacity of D-psicose, a composition and application. Specifically, the metagen is combined with the D-psicose, so that the hypoglycemic effect is remarkably improved. According to the D-psicose and the preparation method of the D-psicose, the metagen obtained through fermentation of the Lactobacillus gasseri co-1 has a synergistic effect with the D-psicose, so that the blood glucose metabolism can be more effectively regulated. In addition, the positive effect of the postbiotics in the aspect of intestinal health further enhances the overall hypoglycemic ability. The metabiotics can improve the intestinal environment related to metabolic diseases by enhancing the barrier function of the intestinal tract and regulating the flora of the intestinal tract. The multi-layer action mechanism not only is beneficial to the control of blood sugar, but also can improve the symptoms of the early stage of diabetes mellitus and effectively prevent or delay the development of diabetes mellitus, thereby having a good practical application value.

The present invention discloses a lysate for animal tissue and a method for using the same in CUT&Tag. The present invention relates to the fields of biotechnology and epigenetic technology, and specifically provides a lysate suitable for animal tissue and a method for using the same in CUT&Tag. The lysate contains an appropriate amount of mild non-ionic detergent NP40 (or CA630) and Triton X-100, which can efficiently lyse tissue clumps while ensuring the integrity of cell nuclei, promote the rapid release of cell nuclei, and thereby obtain cell nuclei suitable for the construction of tissue CUT&Tag libraries. This makes it possible to implement CUT&Tag technology at the animal tissue level, and has great application advantages and high commercial value in the field of epigenetic technology research.

The application discloses a data analysis method in the field of epigenetics: a quantitative analysis method of RNA 5-methylcytosine modification on splicing isomers. The method can solve the problem of how to quantitatively analyze RNA 5-methylcytosine modification on the level of gene splicing isomers, and the technical points include: converting the data set generated by the RNA-BSseq sequencing technology into a virtual RNA-Seq data set and marking the data in which RNA 5-methylcytosine modification occurs; estimating the expression amount of the splicing isomer in the virtual data set, and calculating the mean and variance thereof by using the bootstrap technology; estimating the RNA 5-methylcytosine modification level of the splicing isomer, and calculating the mean and variance of the RNA 5-methylcytosine modification level of the splicing isomer by using the Delta technology; and based on the normal distribution, testing whether there is a significant difference in the estimated RNA 5-methylcytosine modification level of the splicing isomer under different conditions. The application helps to reveal the regulation mechanism of RNA 5-methylcytosine modification in physiological processes and pathological changes.

The application discloses a solution for separating fat cell nucleus and cytoplasm, and the solution comprises solution 1 and solution 2; wherein the solution 1 comprises 8-12 mM hydrochloride buffer, 0.03-0.15% Tween-20, 8-12 mM sodium chloride, 2-4 mM magnesium chloride, 0.8-2% bovine serum albumin and 0.4-0.6% ethyl phenyl polyethylene glycol; the solution 2 comprises 8-12 mM hydrochloride buffer, 8-25 mM sodium chloride, 1-15 mM magnesium chloride and 0.8-2.5% bovine serum albumin; the percentage is volume percentage. The application can realize the research on all cell types of fat tissues, greatly reduces the required amount of fat tissues and improves the possibility of carrying out single cell research. The application can be used for both transcriptome research and epigenetic research.

The purpose of the present invention is to provide molecules that induce, in cells, the decomposition of proteins associated with epigenetic modification, particularly enzymes (e.g. DNA methyltransferase) that catalyze methylation of nucleic acids.　More specifically, the purpose of the present invention is to provide a compound represented by formula (I) or a salt thereof, or a solvate or a hydrate of the same. [In formula (I), A is a double-stranded nucleic acid and includes, in one strand, at least one 5'-CpG-3' sequence in which the 5-position of cytosine (C) has been methylated, the C of the 3'-GpC-5' that is complementary to said 5'-CpG-3' may be a modified base for covalently bonding DNMT1 to A, L is a hydrocarbon that may include oxygen, sulfur, phosphorus, or nitrogen, and B is a ligand molecule of an E3 ubiquitin ligase.]

The invention discloses an experimental method for researching the influence of a pulse electromagnetic field on MC3T3-E1, and relates to the technical field of biomedical engineering. Comprising the following steps: S1, culturing cells; s11, preparation of an MC3T3-E1 cell is carried out; s12, carrying out cell passage; s2, pulse electromagnetic field treatment; s21, setting a conventional culture group and a pulse electromagnetic stimulation group, taking a third generation of MC3T3-E1 cells, rinsing the MC3T3-E1 cells twice with a PBS solution free of calcium and magnesium ions, and counting the MC3T3-E1 cells after the MC3T3-E1 cells are prepared into a cell suspension; respectively inoculating into 25ml cell culture flasks and dividing into three groups: a conventional culture group, a conventional culture and pulse electromagnetic stimulation applying group and a conventional culture and pulse electromagnetic stimulation applying group. The invention provides an important theoretical basis for treating osteoporosis by clinically applying the PEMFs; the potential molecular mechanism of PEMFs is disclosed from the perspective of epigenetics methylation regulation.