648 results about "Cotton bollworm" patented technology

The invention discloses cyano phthalic diamide compounds, a preparation method thereof and use thereof as an agricultural chemical pesticide. The compounds are represented by a structural formula (1), wherein the substituent groups are defined in the description. The phthalic diamide compounds of the invention have wide spectrum pesticidal activities and are effective for epidoptera; particularly, the compounds have high activities for plutella xylostella, cotton bollworm, prodenia litura and the like, and a better effect can be achieved with a small dose of the phthalic diamide compounds.

The invention discloses a corn planting and cultivating method. The corn planting and cultivating method includes the following steps of selecting a corn variety and conducting soil preparation before planting, arranging wide rows and narrow rows at intervals, sowing corn, completing sowing, pipe paving, film paving and soil covering through an air suction type precision seeder, and conducting field management in the seedling stage, the ear stage and the flower stage, wherein the width of each wide row ranges from 85cm to 95cm, the width of each narrow row ranges from 25cm to 35cm, a drip irrigation zone is paved in the middle of each narrow row. According to the corn planting and cultivating method, due to the fact that the method that the wide rows and the narrow rows are arranged at intervals is adopted, the number of drip lines which are used can be lowered. Modifiers are sprayed in the early period of the field management in the seedling stage to control the plant height and the ear position, and the defect that through a conventional planting method, the corn easily falls over due to the fact that the plant height is large and the ear position is high is overcome. Pesticide is sprayed through a self-propelled high-stalk crop boom sprayer in the middle and later period of management to control pests, the influences of natural disasters on corn growth are reduced, and the influences on productivity caused by a large number of European corn borers, cotton bollworms, aphids, leafhoppers and red spiders appearing after corn jointing are eliminated.

The invention discloses a fluoro methoxylpyrazole-containing o-formylaminobenzamide compound shown as a structural formula (I) or agriculturally suitable salts thereof, a synthesis method and application thereof. The fluoro methoxylpyrazole-containing o-formylaminobenzamide compound has the advantages of effectiveness, low toxicity, low cost and environmental safety and can be used for preventingand removing various pests in the fields of agriculture and horticulture or pests parasitized on animals. The fluoro methoxylpyrazole-containing o-formylaminobenzamide compound has very high activityon lepidopteran pests (plutella xylostella, spodoptera exigua and cotton bollworm), hemipteran pests (black peach aphid and aphis craccivora koch), homopteran pests (nilaparvata lugens), dipteran pests (liriomyza trifolii) and leaf beetle pests (phaedon conchleariae). Particularly, good effect on the lepidopteran pests, the hemipteran pests and the dipteran pests can be achieved under the condition of low dosage of the compound.

Belonging to the field of biological control of pests, the invention discloses a Bacillus thuringiensis strain that has a broad spectrum and is highly effective on lepidoptera pests, preparation of its wettable powder and application. The Bacillus thuringiensis YX-1 strain involved in the invention is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 5256. Containing a plurality of insecticidal genes, the strain has a highly effective lethal effect on Cydia molesta, cotton bollworm, fall webworms, Adoxophyes orana Fisher von Roslerstamm, Phalera flavescens Bremer et Grey, carpocapsa pomonella, prodenia litura, plutella xylostella, beet armyworm and other lepidoptera pests. The invention also discloses a method for preparation of wettable powder through the YX-1 strain. The Bacillus thuringiensis YX-1 strain and its wettable powder provided in the invention are mainly used for prevention and treatment of lepidoptera pests on various fruit trees and vegetables, also can be used for prevention and treatment of lepidoptera pests on field crops and garden plants, and have the characteristics of broad spectrum, high efficiency, safety to people, livestock as well as animals and plants, and no environmental pollution, etc.

The invention discloses an insecticidal composition containing metaflumizone, which comprises two active ingredients, namely the A ingredient and the B ingredient, wherein the A ingredient is the metaflumizone, the B ingredient is one of high-effect cypermethrin, cyhalothrin, profenofos, trichlorfon, diafenthiuron, pleocidin, tebufenozide, hexaflumuron, chlorfenapyr, pyridaben, acetamiprid, isoprocarb, dimehypo and padan, and the weight ratio of the A active ingredient to the B active ingredient is 1: 50-10: 1. The insecticidal composition is used for controlling lepidoptera pests in vegetables, rice, fruit trees, cotton and other crops, such as diamond-back moth, beet armyworm, rice leaf roller, chilo suppressalis, yellow rice borer, leaf roller, cotton bollworm and the like. The two active ingredients are mixed, the cotoxicity coefficient (CTC) value of the mixture is calculated according to the Sun Yunpei method, the insecticidal composition shows obvious synergy, the dosage of theactive ingredients can be reduced, and the generation of resistance to a new medicament, namely chlorantraniliprole can be deferred.

The invention provides a broad-spectrum rod-shaped viral pesticide produced by substitute hosts, the components and the weight proportion of which are: 20 billion PIB / ml virus suspension 2.5 to 15 percent, 60nm silicon dioxide 0.1 to 0.5 percent, aromatic amino acid 0.1 to 0.15 percent, refined fluorescent whitening agent 0.5 to 1 percent, sodium lignin sulfonate 0.1 to 1 percent, chlorfluazuron 0.01 to 0.1 percent, glycerol 1.0 to 2.0 percent, and remaining no sterilized water; the virus suspension is received by the infection of nuclear polyhedrosis virus of cabbage armymoth and the substation for the host---cotton bollworm or beet armyworm. The pesticide not only maintains the biological pesticide properties of viral pesticides and has no harms to human beings and higher animals, but also is suitable for the the massive employments on vegetables, and has the wide insect disinfestation spectrum. Therefore, a broad-spectrum viral pesticide can prevent and control various harmful insects while with only a rod-shaped virus. The invention has the advantages of good insecticidal effect, fast insecticidal speed, and very good application prospect.

The invention discloses an insect-fusion-resistant gene, coding protein, a carrier and application thereof. The insect-fusion-resistant gene is formed by using a connecting peptide coding gene to connect a nucleotide sequence of coding BT insecticidal crystal protein Cry1Ab with coding BT trophophase insecticidal protein Vip3A, the connecting peptide amino acid sequence is shown as SEQ ID NO:1. Insecticidal activity of the insect-fusion-resistant gene is improved, and insecticidal spectrum is wider. The insect-fusion-resistant gene can kill main lepidopterous insects like armyworm, Spodoptera litura, Ostrinia furnacalis, cotton bollworm and Spodoptera exigua of paddy and maize. In addition, the insect-fusion-resistant gene can effectively slow down insect resistance.

The invention discloses a pest and glyphosate resistant expression vector, and a plasmid and an application thereof. The expression vector contains a pest-resistant gene cry1Ab, a pest-resistant gene cry2Ab and a glyphosate-resistant gene G10evo. The vector simultaneously expresses Cry1Ab, Cry2Ab and G10evo proteins, and transgenic crops obtained by transferring crops with the vector can simultaneously resist cotton bollworms, beet armyworms, corn borers, striped rice borer and other main lepidoptera pests of rice, corn and cotton, so the insecticidal spectrum is widened, the pest resistance is effectively delayed, the transgenic crops have glyphosate resistance, the transgenic crops with composite properties accord with the current development direction of transgenic crops, and demands of large-scale agricultural production are met.

The invention discloses a Bacillus thuringiensis MB-15 strain which has a broad spectrum and high virulence on vegetable lepidoptera pests, and the preservation number of the strain is CGMCC No. 5255. The strain can form a diamond insecticidal crystal protein, contains cry1I, cry1Ac, cry2Ac and vip3Aa genes, and has high-efficiency insecticidal activity on plutella xylostella, Pieris brassicae, beet armyworm, Spodoptera litura, Helicoverpa armigera and other lepidoptera pests. The invention also relates to a preparation method of the wettable powder of strain. The wettable powder comprises the following content of main components: 40.0-50.0% of Bacillus thuringiensis MB-15 strain powder, 5.0-10.0% of a dispersant, 5.0-10.0% of a wetting agent, 0.5-1.0% of a stabilizer, 0.5-1.0% of an ultraviolet light protection agent, and 28.0-49.0% of a carrier. The wettable powder can be used to control a plurality of lepidoptera pests on vegetables, and has the characteristics of broad spectrum, high efficiency, and safety, etc.

The invention discloses a c DNA (Complementary Desoxvribose Nucleic Acid) of a cotton bollworm COPI Beta gene and an application of the c DNA. A COPI Beta si (Small Interfering) RNA of a cotton bollworm is designed and synthesized, so that the expression of the COPI Beta gene is blocked after the COPI Beta si RNA is fed to the cotton bollworm, thus the death rate of the cotton bollworm is improved. Therefore, the biological prevention and control of the cotton bollworm is realized.

A pesticidal composition in the field of composite pesticides is disclosed in the invention. The effective components of the pesticidal composition comprise sulfoxaflor and amide pesticides, wherein, the weight ratio of sulfoxaflor to amide pesticides is 1-80:80-1, preferred 1-45:35-1. The total content of the effective components accounts for 1-90wt% of the pesticidal composition, preferred 1-50%, and the rest are auxiliary components which are allowed and accepted in pesticides. The pesticidal composition can be prepared into dosage forms, such as missible oil, suspending agent, wettable powder, water dispersible granule, emulsion in water and micron emulsion by known methods, and has substantial effects on controlling Lepidopterous pests such as diamondback moth, beet armyworm, prodenia litura, cotton bollworm, Chilo suppressalis, mealybugs and the like, and homopteran pests with piercing-sucking mouthparts such as plant hopper, aphid, whitefly, leafhopper and the like.

The invention discloses an insecticidal composition for agricultural insects, which contains two active ingredients: the first active ingredient is chlopyrifos, the second active ingredient is 3-chlorine-N<1>-(2-methyl-4-heptafluoroisopropyl phenyl)-N<2>-(1-methyl-1-cyano ethyl) phthalic diamide, and the weight ratio of the first active ingredient to the second active ingredient is (1-50):(20-1). The insecticidal composition is suitable for controlling insects such as crop prodenia litura, beet armyworms, plutella xylostella, chilo suppressalis walkers, rice leaf rollers and cotton bollworms, and is superior to a single insecticide in quick-acting property and persistent-acting property; the use amount and the use cost of the insecticide are reduced; and the environmental protection and comprehensive control of the agricultural insects are facilitated.

The invention discloses an insecticidal composition containing chromafenozide and amide. The insecticidal composition contains active component A and active component B. The active component A is selected from chromafenozide, the active component B is any compound selected from the following: flubendiamide, chlorantraniliprole and cyantraniliprole, and the active component A and the active component B are in a weight ratio of 1:70-70:1. The composition provided in the invention can control a plurality of pests, has an obvious synergistic effect, expands the insecticidal spectrum, and has high activity on plutella xylostella, beet armyworms, Pierisrapae Linne, tea tussock moth, Euproctis pseudoconspersa, peach fruit moths, Lithocolletis ringoniella, Olethreutidae, Archips fuscocupreana Walsingham, Pandermis heparana, cotton bollworms, Adoxophyes orana Fischervon Roslerstamm, Cnaphalocrocis medinalis Guenee, tryporyza incertulas, Chilo suppressalis and naranga aenescens moore. Also, the insecticidal composition can reduce the dosage of pesticides, reduce pesticide residue in crops, and alleviate environmental pollution.

The invention discloses a high-yield cotton cultivation method. The high-yield cotton cultivation method includes the steps: using a constant value mode of 1.5m X 4m and 3m X 4m as spacing in the rows and spacing between rows of fruit trees in jujube-cotton intercropping; before cotton seeding, topdressing and irrigating for the first time on the last twenty days of March, implementing autumn ploughing and winter irrigation, and completing fertilization, land preparation and weed control before autumn ploughing; selecting high-quality seeds, and starting to seed on April 1st and April 5th , wherein seeding depth is 2-3cm, seeding rate is 60-75kg / hm2, 2-3 seeds are sown in each hole, and earthing depth is 1.0-1.5cm; starting final singling when one true leaf appears, intertilling timely, using mepiquat chloride 9 to control the plant height within a range of 65-70cm, and subjecting cotton to topdressing, irrigation, topping and collection; preventing pests such as tetranychus, aphid, thrips and cotton bollworm in jujube-cotton intercropping cropland. By means of the high-yield cotton cultivation method, land utilization rate is fully increased, adverse impact of jujubes on cotton intercropped with the jujubes is lessened, yield of intercropped cotton is maintained and improved, high yield and high efficiency of the cropland can be achieved finally, and consequently income increase is facilitated for farmers.

The invention relates to a method for extracting cottonseed protein. The method comprises the following steps: (1), smashing cottonseeds; (2), treating the cottonseeds with ultrasonic waves; (3), extracting active enzyme in cotton bollworms; (4), performing enzymolysis to cottonseed meal; (5), increasing the temperature, inactivating the enzyme, and centrifugating to obtain supernatant liquid andfilter residue; (6), adding water to flush the filter residue, and mixing the liquid after the filter residue is flushed with the supernatant liquid obtained in the step (3); and (7), removing impurities from the mixed supernatant liquid through a micro filter membrane, condensing through a nanofiltration membrane, and drying to obtain the powdery cottonseed protein. The method provided by the invention can ensure a higher extraction rate in the production of cottonseed protein, can enable the product to have a stronger functional activity, and meanwhile solves the problem that the conventional biological enzymolysis process scheme requires to purchase costly protease, resulting in a high production cost.

The invention relates to a spinetoram compounded insecticidal composition and belongs to the field of pesticide compounding. Active components of the spinetoram compounded insecticidal composition comprise spinetoram and chitin synthesis inhibitor compounds. A weight percentage of the spinetoram to the chitin synthesis inhibitor compounds is (1 to 90): (90 to 1), and preferably, the weight percentage is (1 to 45): (60 to 3). The spinetoram compounded insecticidal composition comprises 1 to 90% by mass of the active components, and preferably, comprises 3 to 7% by mass of the active components and the balance auxiliary ingredients which can be used in pesticides and are acceptable. The spinetoram compounded insecticidal composition can be processed into emulsion in water, microemulsion, missible oil, a suspending agent, wettable powder and water dispersible granules by known methods. The spinetoram compounded insecticidal composition has the advantage that through the active components of spinetoram and the chitin synthesis inhibitor compounds, control of lepidopterous, thysanopterous and homopterous pests such as cnaphalocrocis medinalis guenee, diamondback moth, prodenia litura, cotton bollworm, beet armyworm and thrip is realized.

The invention relates to a pesticide composition with a synergy effect. The pesticide composition is characterized in that effective components comprise methoxyfenozide and emamectin benzoate, wherein the mass ratio of the methoxyfenozide and the emamectin benzoate is 100:1-1:1. The composition has obvious synergy effect and can reduce the pesticide dosage and delay the resistance development. The composition can be used for preventing and controlling lepidoptera pests and is particularly suitable for preventing and controlling noctuidae pests, such as spodoptera exigua, prodenia litura, cotton bollworm, and the like.

The invention discloses a helicoverpa armigera juvenile hormone binding protein (Ha-JHBP) and an encoding gene and application thereof. The helicoverpa armigera juvenile hormone binding protein provided by the invention is a protein shown as (a) or (b), wherein (a) is constituted by an amino acid sequence shown as a sequence 1 in a sequence table, and (b) is obtained by performing substitution and / or deletion and / or addition of one or more amino acid residues on an amino acid residue sequence shown as a sequence 1, is relevant to helicoverpa armigera growth and is derived the sequence 1. The invention further provides an encoding gene of the protein and a functional fragment of the encoding gene. By establishing a dsRNA (double-stranded Ribonucleic Acid) plant expression vector in a way of taking a Ha-JHBP gene as a target gene and expressing a large quantity of dsRNAs of the Ha-JHBP in plants, the insect resistance of transgenic plants is enhanced. The Ha-JHBP has important application values in the fields of biological prevention and control of phytophagous insects, plant insect resistance gene engineering and molecular biology.

The invention relates to a pesticide composition in the field of pesticide combination, wherein the effective ingredients thereof are spinetoram and one of three pyrethroid compounds, the ratio of the two effective ingredients by weight percent is 1 to 80:40 to 0.1, and preferably 3 to 45: 20 to 0.1; the total mass percent of the effective ingredients in the pesticide composition is 1% to 90%, preferably 3% to 50%, the balance is assistant ingredients allowable and acceptable in pesticide preparation, and aqueous emulsion, particle, micro-emulsion, emulsifiable concentrate, suspension, soluble liquid, wettable powder, microcapsule suspension or water dispersible granule can be prepared using known methods; the pesticide combination of the invention can be used for preventing and controlling homopteran and thysanoptera pests such as cnaphalocrocis medinalis guenee, diamondback moth, prodenia litura, cotton bollworm or beet armyworm, thrip, etc.

The invention provides a compound insecticide containing avermectin, active compositions of the invention consist of the avermectin and one of cyhalothrin, profenofos, bifenthrin or triazophos. The invention can be used for controlling insect pest in agriculture or in forest, and especially has good control effect on cotton bollworms, red spider, etc.

The invention discloses a synthetic Bt insecticidal gene mcry1Ab for transgenosis insect-resistant plants. The Bt insecticidal gene mcry1Ab for transgenosis insect-resistant plants provided by the invention is used for encoding mCry1Ab protein. The mCry1Ab protein is b1), b2) or b3) as follows: b1) the protein with the amino acid sequence as shown in the sequence 2 in a sequence table; b2) the fused protein acquired in the manner of connecting a label with a N end or / and C end of the protein as shown in the sequence 2 in the sequence table; and b3) the protein related to plant insect resistance that is acquired in the manner of substituting and / or deleting and / or adding one or more amino acid residue of the amino acid sequence shown as the sequence 2 in the sequence table. An experiment proves that the mcry1Ab gene can easily acquire the transformant with high expression quantity than the cry1Ab gene, the cry1Ab protein, compared with the mcry1Ab protein, has obvious higher insecticidal activity to ostrinia nubilalis, oriental armyworm, cotton bollworm and dichocrocis punctiferalis, and the mcry1Ab gene has an important popularizing value.

The invention discloses an insecticidal composition containing spinetoram. The insecticidal composition comprises a first active ingredient of spinetoram and a second active ingredient of methoxyfenozide or ZJ0967, wherein the weight ratio of the first active ingredient to the second active ingredient is 1:20-15:1. The insecticidal composition can be prepared into agriculturally accepted water dispersible granules, suspending agents, oil emulsion, micellar emulsion or aqueous emulsion; the insecticidal composition has reasonable components and a good insecticidal effect and is low in cost, and the activity and the insecticidal effect of the insecticidal composition is not equal to the simple superposition of the activity of all components; and compared with the presently used single preparation, the insecticidal composition achieves an obvious insecticidal effect as well as obvious synergistic action, has high safety and meets the safety requirements of pesticide formulations. The insecticidal composition has quite high insecticidal activity on cnaphalocrocis medinalis guenee, cotton bollworms, prodenia litura and mythimna separate.

The invention provides a mixed pesticide with spinosad, and the active ingredients thereof are the combination of spinosad and one or more compounds of profenofos, diafenthiuron, and cyhalothrin. The mixed pesticide is used for preventing and curing the damage caused by pests in agriculture or forestry, and has particularly good effects on preventing and curing the damage caused by cotton bollworms, vegetable diamondback moths, cabbage worms, and lima bean pod borers.

The invention discloses a molecular-biological method for quickly distinguishing prodenia litura and other two noctuidae pests (cotton bollworm and tobacco budworm). The method comprises the following steps of: extracting total DNA (Deoxyribonucleic Acid) by adopting a standard phenol-chloroform method or a DNA extraction kit; using a DNA bar code primer to amplify a sequence at the front part of mitochondria CO1 as a label; and comparing diagnostic characters of nucleotides. The molecular-biological method can be used for distinguishing different biotypes and larvae or incomplete individuals of the prodenia litura, the cotton bollworm and the tobacco budworm, has the characteristics of accuracy, quickness, convenience and quickness, and is more accurate and reliable compared with the traditional morphological identification.

The invention discloses a method for distinguishing female and male adults of cotton bollworm based on computer vision technology, after pre-processing the collected images of cotton bollworm of different sexes, the images with feet and antennae removed are obtained, and the color, shape and texture of the images are extracted respectively. The simulated annealing algorithm is used to optimize thedimensionality reduction process. The obtained feature data is trained and tested by the support vector machine classifier, and finally the automatic classification and recognition are realized. Theinvention has the advantages of simple operation, strong robustness and high identification accuracy, and has ideal time performance, and can remarkably improve the identification efficiency of insectsex classification.

The invention relates to a compound with a general formula (A) or an optical isomer, cis-trans isomer or pesticide science-acceptable salt of the compound and a preparation method thereof. The invention also relates to an agricultural composition containing the compound or the optical isomer, cis-trans isomer or pesticide science-acceptable salt of the compound and the application of the agricultural composition. The compound and derivatives thereof have high pesticidal activity on homoptera, lepidoptera and other agricultural and forest pests, such as aphids, plant hoppers, whiteflies, leafhoppers, thrips, cotton bollworms, cabbage caterpillars, diamond back moths, prodenia lituras and armyworms.

The invention relates to insecticide composition in the field of pesticide mixture. The effective constituent of the insecticide composition mixed with amides pesticide is formed by mixture of novel pyrifluquinazon and one of four amide compounds, wherein the weight ratio of the novel pyrifluquinazon to the one of the four amide compounds is 1-90:90-1, and a preferential weight ratio is 1-45:60-5. Mass ration of the effective constituent of the insecticide composition mixed with amides pesticide is 1%-90% and a preferential mass ration is 5%-65%. The other composition of the insecticide composition mixed with amides pesticide is auxiliary component which is allowed and acceptable in pesticide. The insecticide composition mixed with amides pesticide can be prepared into emulsion in water, microemulsion, missible oil, suspending agents, wettable powder, micro-capsule suspending agents, and water dispersible granule. Due to the fact that the effective constituent of the insecticide composition is formed by the mixture of the pyrifluquinazon and the one of the amides compounds, the insecticide composition mixed with the amides pesticide can effectively prevent lepidopteron, such as plutella xylostella, beet armyworms, prodenia litura, cotton bollworms and mealybug and homoptera piercing-sucking mouthpart insects, such as aphids, aleyrodidaes, and leafhopper.

Provided is a method of preparing platinum nano particles by using cotton bollworm karyotype polyhedrin. Inherent structural features of the cotton bollworm karyotype polyhedrin are used, a PtC14 solution is added to a purified cotton bollworm karyotype polyhedral body solution, and the precious metal platinum nano particles which are even in distribution and consistent in diameter of the particles are obtained on the surface of the cotton bollworm karyotype polyhedrin through hatch, ultrasonic wave assistance reduction treatment. Due to the fact that a cotton bollworm karyotype polyhedron carrier has natural specific structures of functional group repetitive distribution, the complex preparation technology of a conventional carrier is avoided, and therefore production cost is reduced. The sizes and distribution of the precious metal platinum nano particles are effectively controlled through control of the alkaline hydrolysis degree of the cotton bollworm karyotype polyhedron, and the method of preparing the platinum nano particles by using the cotton bollworm karyotype polyhedrin can be significantly applied to catalytic fuel cells or biosensing or the like.