58 results about "Cotton bollworm" patented technology

The invention relates to the technical field of microorganisms, in particular to serratia marcescens and application of the serratia marcescens in prevention and treatment of lepidoptera pests. The serratia marcescens CB08 provided by the invention is preserved in the China Center for Type Culture Collection, and the preservation number of the serratia marcescens CB08 is CCTCC (China Center for Type Culture Collection) NO: M 2025266. The strain has a remarkable prevention and control effect on Lepidoptera pests such as spodoptera frugiperda, armyworm, prodenia litura and cotton bollworm, has no pathogenicity on host crops such as corn, is a bacterium with excellent Lepidoptera pest prevention and control potential, has a very wide application prospect, and is expected to bring a new solution to the field of agricultural pest prevention and control.

The invention discloses an insect-resistant transgenic cotton event BrsC35 as well as a detection method and application thereof. According to the invention, an epsps gene, a Cry1Ac gene and a Vip3A gene are transferred into a genome of Zhongmiansui 24 through an agrobacterium-mediated method, so that a transgenic cotton event BrsC35 of which the resistance to lepidoptera insects such as cotton bollworms, ostrinia nubilalis and spodoptera frugiperda is remarkably improved is obtained. The transgenic cotton event BrsC35 is preserved in China General Microbiological Culture Collection Center (CGMCC) on February 17, 2025, and the preservation number is CGMCC No.32181. The transgenic cotton event BrsC35 has been preserved in China General Microbiological Culture Collection Center (CGMCC). The invention also provides a method for identifying whether a plant sample is derived from the transgenic cotton event BrsC35 or an offspring of the transgenic cotton event BrsC35, the transgenic cotton event BrsC35 can be specifically detected, and the transgenic cotton event BrsC35 can be better supervised and managed. The method has an important application value.

The present application belongs to the field of agricultural pest control, and particularly relates to a method for improving the content and insecticidal activity of exogenous insecticidal protein of insect-resistant transgenic cotton. The method is realized by the following steps: maintaining the soil cadmium concentration at 1-5 mg / kg when the cotton is at the three-leaf stage; and maintaining the soil cadmium concentration at 1-4 mg / kg when the cotton is at the five-leaf stage. The method provided by the present application can significantly improve the content and insecticidal effect of exogenous insecticidal protein of insect-resistant transgenic cotton, and enhance the insecticidal activity against cotton bollworms. The method provided by the present application can improve the control effect on pests while controlling cadmium pollution when planting transgenic insect-resistant cotton on land contaminated by a certain concentration of cadmium, and reduce the use of insecticides. Therefore, the present application provides an effective reference for improving the control effect on pests when planting insect-resistant transgenic cotton on land contaminated by cadmium, and has important practical value and significance.

The invention relates to the technical field of agricultural biology, in particular to cotton bollworm salivary protein Ha22, a recombinant vector, recombinant bacteria and application of the cotton bollworm salivary protein Ha22. The invention provides a cotton bollworm salivary protein Ha22. The amino acid sequence of the cotton bollworm salivary protein Ha22 is as shown in SED ID NO. 1. Transient expression of the Ha22 protein on tobacco can cause tobacco cell necrosis, stable overexpression of the Ha22 protein on cotton plants can induce cotton defensive gene response and positively regulate plant insect resistance, and the Ha22 protein has great application value for cotton insect-resistant breeding. In addition, the protein has the active characteristic of an elicitor, so that the purpose of green prevention and control can be achieved by spraying whole protein to plants through in-vitro expression and inducing plant resistance.

This invention discloses aryl heterocyclic compounds containing substituted unsaturated hydrocarbons, their preparation methods, and uses. The compounds have the chemical structure shown in Formula I. The compounds of this invention are used as insecticides, showing particularly good control efficacy against armyworms, diamondback moths, cotton bollworms, corn borers, and rice leaf rollers, and are expected to become novel insecticides.

The invention belongs to the field of biological control, and relates to application and a method of dsRNA of an NRX gene in control of lepidoptera pests. It is found for the first time that the Neurexin-4 gene participates in the growth and development process of the cotton bollworm and the spodoptera frugiperda, the death rate of larvae of the cotton bollworm and the spodoptera frugiperda is remarkably increased through deletion of Neurexin-4 expression, and finally population development is inhibited. The gene can be used for developing RNA biopesticides for preventing and controlling cotton bollworms and spodoptera frugiperda. The invention provides an interference sequence for the cotton bollworm and grassland spodoptera Neurexin-4 gene, the sequence can inhibit the expression of Neurexin-4, and the sequence can be utilized to interfere the expression of the cotton bollworm and grassland spodoptera Neurexin-4 gene and inhibit the growth of the cotton bollworm and grassland spodoptera Neurexin-4 gene.

The application discloses a cotton bollworm nuclear polyhedrosis virus spike period targeted synergistic adjuvant, a pesticide preparation and application, relates to the technical field of agricultural biological pesticides, and the composite adjuvant is prepared from ultraviolet protection and shielding materials, film-forming materials, midgut infection promoters, penetration and spreading agents, system compatible components and carriers.The adjuvant is applied by unmanned aerial vehicle top spraying, and after being compounded with HaNPV, the deposition rate of the virus on the corn tassel can be increased by 3.2 times, the retention amount is increased by 5.62 times, the destruction rate of the midgut peritrophic membrane of the cotton bollworm larvae is more than 90%, and the mortality of the 3rd instar cotton bollworm larvae is increased to 87.72%.The application solves the two technical bottlenecks of the virus pesticide, i.e., 'difficult coverage of the tassel target' and 'low midgut infection efficiency', and provides key technical support for green and precise prevention and control of the cotton bollworm in the corn tassel period.

The invention discloses an insecticidal composition containing entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and relates to the technical field of biological insecticide, the active ingredients of the insecticidal composition are entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and the entomopathogenic virus is nuclear polyhedrosis virus of baculoviridae. The weight ratio of the virus to the jasmonic acid methyl ester or jasmonic acid is 1: (0.5-1000), and the insecticidal composition can be prepared into dosage forms such as a suspending agent, water dispersible granules, wettable powder and the like. The invention also provides a method for preventing and treating lepidoptera pests, which comprises the step of applying the entomopathogenic virus and methyl jasmonate or jasmonic acid to target pests or a living environment of the target pests in a combined manner, and the combined application is simultaneous application or successive interval application. The insecticidal composition has a synergistic effect on lepidoptera pests such as cotton bollworms and beet armyworms, can obviously reduce the food intake, accelerate the death of pests and improve the field control effect, and has a wide application prospect.

The invention provides an insect-resistant and herbicide-resistant gene of corn and application of the insect-resistant and herbicide-resistant gene to cultivation of insect-resistant and herbicide-resistant corn, and belongs to the field of plant disease resistance. Experiments prove that the expression vector for simultaneously expressing the Cry1Ab, Cry2Ab and cp4-epsps genes is constructed for the first time, transgenic crops obtained by transforming crops with the vector can simultaneously resist Lepidoptera pests such as ostrinia nubilalis and cotton bollworm and have good glyphosate resistance, the insect-resistant and herbicide-resistant application of the transgenic crops is widened, and the transgenic crops have broad application prospects. The method has a wide application prospect in corn genetic breeding improvement.

The application belongs to the technical field of biology, and particularly relates to a dsRNA of a target Helicoverpa armigera HaPburs gene and application thereof in prevention and treatment of the Helicoverpa armigera. HaPburs RNA interference mediated knockdown reduces the transcript level by 60.01%, and induces morphological abnormalities of pheromone glands (PG), which are irregular black protrusions; the silencing HaPburs reduces the main pheromone component Z 11-16: Ald content, while the male insect attraction rate and mating rate, cumulative egg production also significantly decrease, and the transcript level of the sex pheromone biosynthesis related genes HaPKA and HaACC is also significantly reduced. It is shown that HaPburs the genes participate in PG morphogenesis and regulate sex pheromone biosynthesis through a cAMP-PKA-ACC regulatory axis, thereby affecting the reproductive communication of the Helicoverpa armigera. The application provides a novel target and technical scheme for green and efficient prevention and control of the Helicoverpa armigera, and has important research and development value and application prospect.

The application of broccoli genes BoSULTR1;1 and BoSULTR1;2 in improving plant stress resistance belongs to the technical field of plant genetic engineering. In order to solve the technical problem of how to improve the stress resistance of broccoli plants, the application provides the broccoli genes BoSULTR1;1 and BoSULTR1;2, and the application uses an expression vector or a recombinant bacterium overexpressing BoSULTR1;1 and BoSULTR1;2 genes to prepare a transgenic broccoli plant. The stress resistance related detection results prove that the BoSULTR1;1 and BoSULTR1;2 genes have the ability to improve the resistance of the transgenic broccoli plant to cotton bollworm, pathogenic bacteria and drought. The transgenic material obtained by the application has important theoretical significance and practical value for accelerating the breeding process of stress-resistant plants and improving the breeding efficiency.

The invention belongs to the technical field of gene editing, and discloses a cotton bollworm UGT33 gene cluster targeted knockout sgRNA, the sgRNA comprises sgRNA1 and sgRNA2, the sequence of the sgRNA1 is 5 '-, and the sequence of the sgRNA2 is 5'-.

The invention discloses a cotton bollworm larva feeding antifeedant and application of special valeralose derived from plants in cotton bollworm larva feeding refusal, L-arabinose has antifeedant activity on cotton bollworm larvae, and the L-arabinose also has a function of inhibiting the sum of midgut digestive enzymes of the cotton bollworm larvae; l-arabinose is stable in chemical property and not easy to degrade in nature, not only can inhibit feeding of young larvae, but also can inhibit feeding of young cotton bollworm larvae. As a plant source antifeedant, the L-arabinose has a remarkable antifeedant effect on cotton bollworm larvae, is convenient to use and low in cost, has no harm to the environment and no influence on plant growth and development, is more suitable for being mixed with other types of plant source pesticides for application, and can be developed and applied as an efficient, economic, simple, convenient and novel plant source pesticide.

The invention discloses an RNA biopesticide as well as application and a preparation method thereof. A first aspect of the present application discloses an RNA biopesticide comprising a double-stranded RNA molecule, the glycosyl moiety of at least one nucleotide of the double-stranded RNA molecule having a 2 '-OMe modification. Research results show that when 2 '-OMe-NTPs is doped into the dsRNA, the dsRNA can bear ultraviolet radiation for a long time, and the stability of the dsRNA can be remarkably improved in a high-temperature environment. More importantly, the composition is prevented from being degraded by nuclease in leaf surfaces or lepidoptera pest cotton bollworm intestinal juice environment, and the control effect on cotton aphids and cotton bollworms is remarkably improved. Therefore, the stability of the dsRNA and the RNAi efficiency are improved by modifying the binding site and only depending on the structural change of the dsRNA under the condition of not introducing other materials.

The invention belongs to the field of gene engineering, and particularly relates to a bacillus thuringiensis binary toxin gene and application thereof in cotton bollworm prevention and control. The Cry-Tpp binary toxin combination with inhibitory activity on lepidoptera insects is identified for the first time, gene resources with commercial potential are provided for development of novel microbial insecticides and insect-resistant transgenic crops, and a new direction is provided for enhancing the activity of the Cry toxoid, artificially modifying the Cry toxoid and solving the problem of insect resistance. The method is of great significance to deep development and development innovation of Bt resources.

This application belongs to the field of biopesticides and provides the application of HearNPV-miR-hr5b in the control of cotton bollworm. The nucleotide sequence of HearNPV-miR-hr5b is CGGGCAAAUUUCAAAUAAA. HearNPV-miR-hr5b of this application can enhance the replication and accumulation of HearNPV in the host cotton bollworm, accelerate the larval mortality rate, and increase the rate of resistant cotton bollworm control. It can be used to develop HearNPV synergists and has good application prospects in the biological control of cotton bollworm.

The invention relates to an in-situ binding method for evaluating binding of Bt toxin Cry1Ac and midgut epithelial cells of cotton bollworm larvae. The method comprises the following steps: incubating and culturing midgut tissues of larvae of lepidoptera pests and a Bt toxin-containing Sf-900 II cell culture solution, carrying out immunostaining, observing and photographing through a laser confocal microscope, and finally carrying out quantitative processing on a fluorescence image by utilizing ImageJ software so as to calculate an average unit area fluorescence signal value. And further evaluating the difference between the binding amounts of the midgut epithelial cells and the Bt toxins of the larvae of different strains. The method can intuitively and accurately observe and evaluate the binding condition of the Bt toxin and the midgut epithelial cells on the premise of retaining the original structure and physiological environment of the midgut tissue of the larvae, provides direct data support for Bt resistance research, assists in analyzing the pest Bt resistance mechanism, has important significance in formulating resistance early warning and treatment strategies, and has a wide application prospect. The method is suitable for the biotechnical fields of Bt toxin insecticidal mechanism research, lepidoptera pest drug resistance mechanism exploration and the like.

The invention relates to the technical field of preparation of phenylisoxazole derivatives, in particular to phenylisoxazole derivatives containing pyrazole or triazole amide as well as a preparation method and application of the phenylisoxazole derivatives containing pyrazole or triazole amide. The phenylisoxazole derivative containing pyrazole or triazole amide provided by the invention has good insecticidal activity on lepidoptera pests such as plutella xylostella, spodoptera frugiperda, beet armyworm, cotton bollworm and the like, and can be used as a pesticide active component to be applied to prevention and control of agricultural pests and preparation of insecticides.

The application provides a human-derived genetically engineered antibody with targeted simulation of Bt Cry1C toxin structure and insecticidal function and a high-activity mutant of the human-derived genetically engineered antibody, nucleotide sequences and amino acid sequences of the human-derived genetically engineered antibody are respectively shown as SEQ IN NO. 1 and SEQ IN NO. 2, nucleotide sequences and amino acid sequences of the mutant are respectively shown as SEQ IN NO. 3 and SEQ IN NO. 4; after the antibody and the mutant are expressed in prokaryotes, the LD50 of corresponding proteins to Plutella xylostella larvae is respectively 38.9% and 46.8% of the original Bt Cry1C toxin, the LD50 of corresponding proteins to cotton bollworm larvae is respectively 32.9% and 37.4% of the original Bt Cry1C toxin; the two can be respectively used as coating antigens in an IC-ELISA detection method to detect Bt Cry1C toxin with high sensitivity; the antibody and the mutant can be used as antigen analogue materials to replace the structure of Bt Cry1C toxin, not only can be used as new protein insect-resistant materials to have potential application value in green control of agricultural pests, but also can be used for detection of Bt Cry1C toxin residues in the environment and food samples, and have wide application value.

The invention discloses serratia marcescens with efficient insecticidal activity on lepidoptera larvae and application of the serratia marcescens. Researches show that fermentation liquor or diluent of the obtained serratia marcescens Sm001 strain has a good insecticidal effect on beet armyworms, cotton bollworms, prodenia litura and armyworms, for example, the corrected death rate of the beet armyworms treated for 3 days is as high as 96.67%. Therefore, the strain has a very good biological prevention and control effect, can be used for preventing and controlling agricultural pests, and is beneficial to reducing the use of chemical pesticides and the like.

The invention relates to the field of biological prevention and treatment, in particular to a stomach toxicity insecticidal active polypeptide as well as a preparation method and application thereof, and more particularly relates to the stomach toxicity insecticidal active polypeptide as well as polynucleotide, an expression cassette, a preparation method, a use method and application thereof. The invention relates to a polynucleotide molecule for coding a stomach toxicity insecticidal active polypeptide. The polynucleotide molecule is selected from one of the following nucleic acid molecules: a nucleic acid molecule consisting of a nucleotide sequence selected from SEQ ID NO: 1; nucleic acid molecules encoding a polypeptide consisting of the amino acid sequence of SEQ ID NO: 2. The Pc-1 has wide and efficient insecticidal activity on various lepidoptera pests such as prodenia litura, cotton bollworm and the like, so that the Pc-1 has broad-spectrum application potential, and an important core molecular entity is provided for developing a new generation of efficient, low-residue and good-environmental-compatibility polypeptide biological insecticide.

The present invention relates to the use of fatty acid esters in deterring oviposition by cotton bollworms. In particular, the use of a combination of methyl palmitate, methyl stearate and methyl oleate in deterring oviposition by cotton bollworms.

The invention provides a pest and disease damage accurate monitoring and recognition system, and relates to the field of agricultural intelligent robots, the pest and disease damage accurate monitoring and recognition system comprises a searchlighting recognition system, a walking mechanism, a mechanical arm assembly, an electric water pump, a central control system, a liquid medicine box, a chassis and a sensing system.The specific recognition control method comprises the steps that 1, addition correction is conducted firstly, then binocular stereoscopic vision calibration is conducted, and a calibration result is obtained; 2, monitoring insect pests of the crops in real time by adopting an improved YOLOv8 algorithm, and identifying corresponding types and numbers of the insect pests; 3, acquiring three-dimensional space coordinates of the insect pest image through the insect pest image; and 4, controlling the dosage of the pesticide according to the three-dimensional space coordinates and the corresponding types and quantity of the insect pests. The system can carry out all-weather and all-directional insect pest recognition, is high in detection precision and high in real-time performance, and effectively improves the accuracy and timeliness of cotton bollworm pest prevention and control.

Method for simultaneously preventing pink bollworm and American bollworm, the method including at least: a step in which cotton seedlings are planted in a field 21-41 days after the date of occurrence of the adult stage of the pink bollworm in the case of the first generation, and 1-29 days after the date of occurrence of the adult stage in the case of second and subsequent generations; and a step in which an extended-release pheromone preparation including at least Z,Z / Z,E-7,11-hexadecadienyl acetate, which is a sexual pheromone substance for pink bollworm, and Z-11-hexadecenal, which is a sexual pheromone substance for American bollworm, is implanted in the field 2-15 days before the expected date of occurrence of the adult stage of the next generation of pink bollworm, the expected date derived from the effective integrated temperature, and the sexual pheromone substances in the extended-release pheromone preparation are released in the field.

This invention belongs to the field of insecticide ecological safety evaluation technology, and specifically relates to a rapid evaluation method for insecticide safety based on parasitic wasp embryonic development. This invention uses the embryos of the cotton bollworm *Ichthyophthirius multifiliis* as the research object, selecting embryonic development status as the core evaluation indicator. Combining the statistical significance and quantitative change range of this indicator with the control group, a standardized experimental measurement method and a three-level ecological safety classification rule are established. By selecting commonly used agricultural insecticides bromfenac and abamectin, ecological safety evaluations were conducted. Compared with the control group, the number of normal embryos with bromfenac decreased by approximately 17%, classifying it as a medium-risk insecticide; the number of normally developing embryos with abamectin decreased by approximately 23.21%, classifying it as a high-risk insecticide. This application fills the technical gap in the ecological safety evaluation of insecticides at the parasitic wasp embryonic stage, improves the evaluation system for non-target natural enemies, and can be directly applied to pesticide research and development, insecticide screening, and field application guidance, contributing to the green development of agriculture.

The application discloses application of an epidermal protein gene SlituCP26 in prevention and treatment of Spodoptera litura, and relates to the technical field of biotechnology.The nucleotide sequence of the SlituCP26 gene is shown as SEQ ID NO:1, the gene is highly expressed in indoxacarb-resistant strains, the dsRNA of the gene is prepared, and the dsRNA is microinjected into the bodies of Spodoptera litura larvae; after the injection, the expression level of the SlituCP26 gene is significantly reduced, the body size of the larvae is obviously reduced, the epidermal thickness is significantly thinned, and the sensitivity to indoxacarb is significantly improved.The application further provides a SlituCP26 gene promoter, and the difference on the sequence amplified through the promoter can be used for detecting the drug resistance of Spodoptera litura.The application confirms that the epidermal protein SlituCP26 gene is involved in the action of Spodoptera litura to insecticides and the formation process of the resistance mechanism, and has theoretical significance for development of new insecticides.

The invention belongs to the field of biological medicine, and relates to a dsRNA compound auxiliary agent and a method and application thereof for preparing RNA biopesticide, BioIon18 biological ions (BI18) are used as a main material for loading dsRNA, and the loaded dsRNA shows relatively strong stability in extreme environments such as RNase A and strong ultraviolet, and can effectively prevent degradation of the dsRNA. Meanwhile, cell uptake of dsRNA can be promoted, and the effect of improving the target gene expression inhibition effect in cotton bollworm larvae is achieved. Other aspect designs of the invention include these BI18 at dsRNA drug complexes suitable for use in the field of ecology, as well as methods of inhibiting expression of target genes by administering these dsRNA drugs, e.g., for use in agricultural pest management.

The application belongs to the field of biological pesticides, and provides application of HearNPV-miR-56 in cotton bollworm control, wherein the nucleotide sequence of the HearNPV-miR-56 is CGGUAACAGAAGCGGUCACG. The HearNPV-miR-56 can improve virus replication amount of cotton bollworm nuclear polyhedrosis virus infected larvae, thereby promoting death, and can be used for developing a cotton bollworm nuclear polyhedrosis virus synergist, and has potential application prospect in biological control of cotton bollworm.

The invention relates to the technical field of pesticide chemistry, and discloses an isoxazoline derivative containing a triazole thioether structure and preparation and application of the isoxazoline derivative, three active fragments of isoxazole, triazole and thioether are innovatively subjected to structural splicing synthesis, and the preparation process is clear in step, mild in reaction condition, excellent in intermediate yield and easy to industrially amplify. The insecticidal activity of the derivative on major agricultural pests such as plutella xylostella, spodoptera frugiperda and ostrinia nubilalis reaches 100%, the activity of the derivative is equivalent to that of fluxazole amide, the derivative can prevent and treat various pests such as cotton bollworm, aphid and rice planthopper, and the derivative is not applied in the field, has no drug resistance of the pests, has the advantages of broad spectrum, high efficiency, difficult generation of drug resistance and the like, can be used for preparing insecticides, and has wide application prospects. A brand new solution is provided for green prevention and control of agricultural pests.