82 results about "Cotton bollworm" patented technology

The invention relates to application of a GhRPS20B gene in improvement of insect resistance of cotton bollworm and insect-resistant breeding, and belongs to the technical field of agricultural biology. The invention provides an application of a GhRPS20B gene as shown in SEQ ID NO.1 in improving the disease resistance of cotton bollworm diseases of cotton. Experiments show that the defense mechanism of jasmonic acid (JA) in cotton can be activated by enhancing the transcriptional level of the GhRPS20B gene in a plant body. The GhRPS20B gene is over-expressed in cotton through an agrobacterium tumefaciens-mediated genetic transformation technology, so that the expression level of the GhRPS20B gene in a target plant is enhanced, the plant with enhanced resistance can be effectively cultivated, and the natural defense capability of the plant to cotton bollworms is improved.

The invention provides an insecticide composition, an insecticide preparation and preparation and application of the insecticide composition and the insecticide preparation, and relates to the technical field of pesticides. The pesticide composition provided by the invention comprises a component A and a component B, the component A is (1S, 2R, 4S)-1, 3, 3-trimethyl bicyclo [2.2. 1] heptane-2-yl-4-(tert-butyl) benzoate, the component B is chlorantraniliprole, tetrachlorantraniliprole or emamectin benzoate, and the mass ratio of the component A to the component B is 1: (0.25-4). The insecticide composition provided by the invention has relatively high toxicity to lepidoptera pests such as cotton bollworms, plutella xylostella and corn borers, has a remarkable synergistic effect, can remarkably reduce the dosage of chlorantraniliprole, tetrachlorantraniliprole and emamectin benzoate, realizes application reduction and synergy of pesticides, and delays the development of pest resistance to drugs; and all the components are low-toxicity and low-residue compounds and are easy to degrade in the environment.

The invention relates to the technical field of microorganisms, in particular to a cotton bollworm metarhizium reesei MRDL2024 strain and application thereof.The classification name of the metarhizium reesei MRDL2024 is metarhizium reesei MRDL2024, and the preservation number of the metarhizium reesei MRDL2024 is CGMCC No.41555. The metarhizium reesei MRDL2024 is easy to culture, rapid in growth, large in sporulation quantity, high in spore germination rate and easy to culture and breed in a large amount indoors, and the metarhizium reesei MRDL2024 can be widely applied to the field of agricultural production. The fungus is a biocontrol fungus which can be used for preventing and controlling the pest cotton bollworm which harms crops such as corn and tobacco, has the characteristics of strong pathogenicity to the larva of the cotton bollworm, safety to the environment and difficulty in causing the pest to generate drug resistance, and can be used for biologically preventing and controlling the cotton bollworm.

The invention belongs to the technical field of pest control, and particularly provides a cotton bollworm nuclear polyhedrosis virus WV104 which is preserved in the China Center for Type Culture Collection (CCTCC) in June 2024, and the preservation number is CCTCC NO: V202461. The virus has the advantages of wide insecticidal spectrum, high toxicity and the like, and has good insecticidal activity on larvae of various lepidoptera pests. The insecticidal composition has a good control effect on oriental tobacco budworms, can remarkably reduce the harm degree of the oriental tobacco budworms and improve the yield and quality of crops, is harmless to the environment and human health, meets the requirements of green agriculture development, and has important significance on development of efficient insect virus insecticides and biological control of pests. The invention further provides a composite suspending agent based on the cotton bollworm nuclear polyhedrosis virus WV104, the composite suspending agent has the characteristics of ultraviolet resistance, high adhesiveness, long lasting period, greenness, health and environmental protection, the prevention effect of the composite suspending agent for preventing and treating oriental tobacco budworms in the field reaches 78% or above, and the composite suspending agent has good application and popularization prospects.

The invention relates to the technical field of microorganisms, in particular to serratia marcescens and application of the serratia marcescens in prevention and treatment of lepidoptera pests. The serratia marcescens CB08 provided by the invention is preserved in the China Center for Type Culture Collection, and the preservation number of the serratia marcescens CB08 is CCTCC (China Center for Type Culture Collection) NO: M 2025266. The strain has a remarkable prevention and control effect on Lepidoptera pests such as spodoptera frugiperda, armyworm, prodenia litura and cotton bollworm, has no pathogenicity on host crops such as corn, is a bacterium with excellent Lepidoptera pest prevention and control potential, has a very wide application prospect, and is expected to bring a new solution to the field of agricultural pest prevention and control.

The invention discloses an amide derivative and its application. The amide derivative is 5-chloro-N-(2-fluoro-3-(2-iodo-4-(perfluoropropane-2-yl)-6-(trifluoromethyl)phenyl)carbamoyl)phenyl)thiophene-2-carboxamide. The amide derivative of the invention has excellent persistence and rapidity in killing and controlling agricultural and forestry pests, especially chewing mouthparts pests such as diamondback moth, beet armyworm, cotton bollworm, etc., and is not easy to produce cross-resistance with existing diamide compounds. Moreover, the safety to mammals is very excellent, and the safety to beneficial insects such as bees is excellent. The product has huge market potential in the field of agricultural and forestry pest control in the future and has very good prospects for promotion and application.

The invention discloses an insect-resistant transgenic cotton event BrsC35 as well as a detection method and application thereof. According to the invention, an epsps gene, a Cry1Ac gene and a Vip3A gene are transferred into a genome of Zhongmiansui 24 through an agrobacterium-mediated method, so that a transgenic cotton event BrsC35 of which the resistance to lepidoptera insects such as cotton bollworms, ostrinia nubilalis and spodoptera frugiperda is remarkably improved is obtained. The transgenic cotton event BrsC35 is preserved in China General Microbiological Culture Collection Center (CGMCC) on February 17, 2025, and the preservation number is CGMCC No.32181. The transgenic cotton event BrsC35 has been preserved in China General Microbiological Culture Collection Center (CGMCC). The invention also provides a method for identifying whether a plant sample is derived from the transgenic cotton event BrsC35 or an offspring of the transgenic cotton event BrsC35, the transgenic cotton event BrsC35 can be specifically detected, and the transgenic cotton event BrsC35 can be better supervised and managed. The method has an important application value.

The invention relates to a method for constructing a cotton bollworm male sterile line and a related transformation event. The invention discloses a method for constructing a cotton bollworm male sterile line. After cotton bollworms containing a Chr16-Vasa-Cas9 transformation event and cotton bollworms containing a Chr5-U6-S2sgRNA transformation event are hybridized to obtain hybrid F1-generation cotton bollworms co-expressing Cas9 and sgRNA, the cotton bollworms show male sterility phenotypes and have no other fitness cost, namely the mating success rate, the mating time and the egg laying amount of the cotton bollworms are not remarkably changed. On one hand, two preferable transformation events are provided for constructing a cotton bollworm male sterile line, and a solid foundation is laid for developing a green and environment-friendly cotton bollworm efficient control strategy without resistance risk; meanwhile, the invention provides a method for carrying out molecular identification on the two transformation events, and an important scientific basis is provided for carrying out identity identification on the transformation events and tracing and supervising the transgenic cotton bollworm.

The invention relates to the field of microbial pesticides, and particularly discloses a microbial pesticide for preventing and treating crop pests and a preparation method thereof. The preparation method of the microbial pesticide for preventing and treating crop pests comprises the following steps: S1, extracting polysaccharide; s2, extracting insecticidal protein; s3, loading: dispersing insecticidal protein, Bt 4D19 polysaccharide, 2-methylimidazole and zinc acetate in a weight ratio of (8-12): (5-10): 500: (50-55) in water, stirring for reaction, filtering to obtain a precipitate, washing and drying to obtain a polysaccharide-protein-metal composite organic framework; s4, pesticide preparation. The microbial pesticide provided by the invention has high insecticidal efficacy, strong stability and good liquid medicine adhesion ability, has an obvious control effect on corn borers and cotton bollworms, has a survival rate of not higher than 10%, is applied after heating heat treatment at 55 DEG C, has a survival rate of not higher than 18%, and is applied after light treatment, and has a survival rate of not higher than 22%.

The present invention discloses a pest trapping device for selectively protecting natural enemies. By utilizing the living habits of Lepidoptera pests, it attracts adult cotton bollworms, adult beet armyworms, adult Spodoptera litura, adult cotton leafrollers, adult cutworms and other Lepidoptera pests for capture to reduce the number of pests. At the same time, by arranging insect-blocking teeth pointing to the center of the attracting cavity on the inner edge of the insect inlet groove of the trapping device, it does not affect the entry of pests into the attracting cavity while preventing pests from escaping from the insect inlet groove. Natural enemy insects such as Adonia variegata, Coccinella undecimpunctata, Stethorus punctillum Weise, and Nabis sinoferus Hsiao, which are smaller in size than pests, can freely enter and exit. While trapping pests, it protects natural enemy insects, solves the problem that existing pest trapping devices are prone to capture natural enemy insects together when trapping pests, resulting in a simultaneous reduction in the number of pests and natural enemy insects and an insignificant control of the pest population, and achieves the purpose of protective biological control, having broad popularization and application value in the field of pest trapping technology.

The present application belongs to the field of agricultural pest control, and particularly relates to a method for improving the content and insecticidal activity of exogenous insecticidal protein of insect-resistant transgenic cotton. The method is realized by the following steps: maintaining the soil cadmium concentration at 1-5 mg / kg when the cotton is at the three-leaf stage; and maintaining the soil cadmium concentration at 1-4 mg / kg when the cotton is at the five-leaf stage. The method provided by the present application can significantly improve the content and insecticidal effect of exogenous insecticidal protein of insect-resistant transgenic cotton, and enhance the insecticidal activity against cotton bollworms. The method provided by the present application can improve the control effect on pests while controlling cadmium pollution when planting transgenic insect-resistant cotton on land contaminated by a certain concentration of cadmium, and reduce the use of insecticides. Therefore, the present application provides an effective reference for improving the control effect on pests when planting insect-resistant transgenic cotton on land contaminated by cadmium, and has important practical value and significance.

The invention relates to the technical field of agricultural biology, in particular to cotton bollworm salivary protein Ha22, a recombinant vector, recombinant bacteria and application of the cotton bollworm salivary protein Ha22. The invention provides a cotton bollworm salivary protein Ha22. The amino acid sequence of the cotton bollworm salivary protein Ha22 is as shown in SED ID NO. 1. Transient expression of the Ha22 protein on tobacco can cause tobacco cell necrosis, stable overexpression of the Ha22 protein on cotton plants can induce cotton defensive gene response and positively regulate plant insect resistance, and the Ha22 protein has great application value for cotton insect-resistant breeding. In addition, the protein has the active characteristic of an elicitor, so that the purpose of green prevention and control can be achieved by spraying whole protein to plants through in-vitro expression and inducing plant resistance.

This invention discloses aryl heterocyclic compounds containing substituted unsaturated hydrocarbons, their preparation methods, and uses. The compounds have the chemical structure shown in Formula I. The compounds of this invention are used as insecticides, showing particularly good control efficacy against armyworms, diamondback moths, cotton bollworms, corn borers, and rice leaf rollers, and are expected to become novel insecticides.

The invention belongs to the field of biological control, and relates to application and a method of dsRNA of an NRX gene in control of lepidoptera pests. It is found for the first time that the Neurexin-4 gene participates in the growth and development process of the cotton bollworm and the spodoptera frugiperda, the death rate of larvae of the cotton bollworm and the spodoptera frugiperda is remarkably increased through deletion of Neurexin-4 expression, and finally population development is inhibited. The gene can be used for developing RNA biopesticides for preventing and controlling cotton bollworms and spodoptera frugiperda. The invention provides an interference sequence for the cotton bollworm and grassland spodoptera Neurexin-4 gene, the sequence can inhibit the expression of Neurexin-4, and the sequence can be utilized to interfere the expression of the cotton bollworm and grassland spodoptera Neurexin-4 gene and inhibit the growth of the cotton bollworm and grassland spodoptera Neurexin-4 gene.

The application discloses a cotton bollworm nuclear polyhedrosis virus spike period targeted synergistic adjuvant, a pesticide preparation and application, relates to the technical field of agricultural biological pesticides, and the composite adjuvant is prepared from ultraviolet protection and shielding materials, film-forming materials, midgut infection promoters, penetration and spreading agents, system compatible components and carriers.The adjuvant is applied by unmanned aerial vehicle top spraying, and after being compounded with HaNPV, the deposition rate of the virus on the corn tassel can be increased by 3.2 times, the retention amount is increased by 5.62 times, the destruction rate of the midgut peritrophic membrane of the cotton bollworm larvae is more than 90%, and the mortality of the 3rd instar cotton bollworm larvae is increased to 87.72%.The application solves the two technical bottlenecks of the virus pesticide, i.e., 'difficult coverage of the tassel target' and 'low midgut infection efficiency', and provides key technical support for green and precise prevention and control of the cotton bollworm in the corn tassel period.

The invention discloses an insecticidal composition containing entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and relates to the technical field of biological insecticide, the active ingredients of the insecticidal composition are entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and the entomopathogenic virus is nuclear polyhedrosis virus of baculoviridae. The weight ratio of the virus to the jasmonic acid methyl ester or jasmonic acid is 1: (0.5-1000), and the insecticidal composition can be prepared into dosage forms such as a suspending agent, water dispersible granules, wettable powder and the like. The invention also provides a method for preventing and treating lepidoptera pests, which comprises the step of applying the entomopathogenic virus and methyl jasmonate or jasmonic acid to target pests or a living environment of the target pests in a combined manner, and the combined application is simultaneous application or successive interval application. The insecticidal composition has a synergistic effect on lepidoptera pests such as cotton bollworms and beet armyworms, can obviously reduce the food intake, accelerate the death of pests and improve the field control effect, and has a wide application prospect.

The invention provides an insect-resistant and herbicide-resistant gene of corn and application of the insect-resistant and herbicide-resistant gene to cultivation of insect-resistant and herbicide-resistant corn, and belongs to the field of plant disease resistance. Experiments prove that the expression vector for simultaneously expressing the Cry1Ab, Cry2Ab and cp4-epsps genes is constructed for the first time, transgenic crops obtained by transforming crops with the vector can simultaneously resist Lepidoptera pests such as ostrinia nubilalis and cotton bollworm and have good glyphosate resistance, the insect-resistant and herbicide-resistant application of the transgenic crops is widened, and the transgenic crops have broad application prospects. The method has a wide application prospect in corn genetic breeding improvement.

The invention discloses a method for improving insect resistance of Bt cotton based on amino acid, which comprises the following step: after the Bt cotton blooms, spraying an amino acid composition on cotton plants. According to the invention, the amino acid composition is sprayed on cotton plants of Bt cotton after blooming so as to improve the insect resistance of the Bt cotton. By means of the method, the Bt insecticidal protein concentration of Bt cotton leaves can be increased by 38.9%-41.2%, the Bt insecticidal protein concentration of cotton bolls can be increased by 25.5%-29.8%, the number of hundreds of cotton bollworms can be decreased by 33.2%-38.6%, the damage of the cotton bollworms to squaring and bolls is decreased by 31.2%-37.6%, the boll weight is increased by 5.5%-6.3%, and the yield is increased by 6.7%-8.3%.

The application belongs to the technical field of biology, and particularly relates to a dsRNA of a target Helicoverpa armigera HaPburs gene and application thereof in prevention and treatment of the Helicoverpa armigera. HaPburs RNA interference mediated knockdown reduces the transcript level by 60.01%, and induces morphological abnormalities of pheromone glands (PG), which are irregular black protrusions; the silencing HaPburs reduces the main pheromone component Z 11-16: Ald content, while the male insect attraction rate and mating rate, cumulative egg production also significantly decrease, and the transcript level of the sex pheromone biosynthesis related genes HaPKA and HaACC is also significantly reduced. It is shown that HaPburs the genes participate in PG morphogenesis and regulate sex pheromone biosynthesis through a cAMP-PKA-ACC regulatory axis, thereby affecting the reproductive communication of the Helicoverpa armigera. The application provides a novel target and technical scheme for green and efficient prevention and control of the Helicoverpa armigera, and has important research and development value and application prospect.

The application of broccoli genes BoSULTR1;1 and BoSULTR1;2 in improving plant stress resistance belongs to the technical field of plant genetic engineering. In order to solve the technical problem of how to improve the stress resistance of broccoli plants, the application provides the broccoli genes BoSULTR1;1 and BoSULTR1;2, and the application uses an expression vector or a recombinant bacterium overexpressing BoSULTR1;1 and BoSULTR1;2 genes to prepare a transgenic broccoli plant. The stress resistance related detection results prove that the BoSULTR1;1 and BoSULTR1;2 genes have the ability to improve the resistance of the transgenic broccoli plant to cotton bollworm, pathogenic bacteria and drought. The transgenic material obtained by the application has important theoretical significance and practical value for accelerating the breeding process of stress-resistant plants and improving the breeding efficiency.

The invention belongs to the technical field of gene editing, and discloses a cotton bollworm UGT33 gene cluster targeted knockout sgRNA, the sgRNA comprises sgRNA1 and sgRNA2, the sequence of the sgRNA1 is 5 '-, and the sequence of the sgRNA2 is 5'-.

The invention discloses a cotton bollworm larva feeding antifeedant and application of special valeralose derived from plants in cotton bollworm larva feeding refusal, L-arabinose has antifeedant activity on cotton bollworm larvae, and the L-arabinose also has a function of inhibiting the sum of midgut digestive enzymes of the cotton bollworm larvae; l-arabinose is stable in chemical property and not easy to degrade in nature, not only can inhibit feeding of young larvae, but also can inhibit feeding of young cotton bollworm larvae. As a plant source antifeedant, the L-arabinose has a remarkable antifeedant effect on cotton bollworm larvae, is convenient to use and low in cost, has no harm to the environment and no influence on plant growth and development, is more suitable for being mixed with other types of plant source pesticides for application, and can be developed and applied as an efficient, economic, simple, convenient and novel plant source pesticide.

The invention discloses an RNA biopesticide as well as application and a preparation method thereof. A first aspect of the present application discloses an RNA biopesticide comprising a double-stranded RNA molecule, the glycosyl moiety of at least one nucleotide of the double-stranded RNA molecule having a 2 '-OMe modification. Research results show that when 2 '-OMe-NTPs is doped into the dsRNA, the dsRNA can bear ultraviolet radiation for a long time, and the stability of the dsRNA can be remarkably improved in a high-temperature environment. More importantly, the composition is prevented from being degraded by nuclease in leaf surfaces or lepidoptera pest cotton bollworm intestinal juice environment, and the control effect on cotton aphids and cotton bollworms is remarkably improved. Therefore, the stability of the dsRNA and the RNAi efficiency are improved by modifying the binding site and only depending on the structural change of the dsRNA under the condition of not introducing other materials.

The invention belongs to the field of gene engineering, and particularly relates to a bacillus thuringiensis binary toxin gene and application thereof in cotton bollworm prevention and control. The Cry-Tpp binary toxin combination with inhibitory activity on lepidoptera insects is identified for the first time, gene resources with commercial potential are provided for development of novel microbial insecticides and insect-resistant transgenic crops, and a new direction is provided for enhancing the activity of the Cry toxoid, artificially modifying the Cry toxoid and solving the problem of insect resistance. The method is of great significance to deep development and development innovation of Bt resources.

This application belongs to the field of biopesticides and provides the application of HearNPV-miR-hr5b in the control of cotton bollworm. The nucleotide sequence of HearNPV-miR-hr5b is CGGGCAAAUUUCAAAUAAA. HearNPV-miR-hr5b of this application can enhance the replication and accumulation of HearNPV in the host cotton bollworm, accelerate the larval mortality rate, and increase the rate of resistant cotton bollworm control. It can be used to develop HearNPV synergists and has good application prospects in the biological control of cotton bollworm.

The invention relates to an in-situ binding method for evaluating binding of Bt toxin Cry1Ac and midgut epithelial cells of cotton bollworm larvae. The method comprises the following steps: incubating and culturing midgut tissues of larvae of lepidoptera pests and a Bt toxin-containing Sf-900 II cell culture solution, carrying out immunostaining, observing and photographing through a laser confocal microscope, and finally carrying out quantitative processing on a fluorescence image by utilizing ImageJ software so as to calculate an average unit area fluorescence signal value. And further evaluating the difference between the binding amounts of the midgut epithelial cells and the Bt toxins of the larvae of different strains. The method can intuitively and accurately observe and evaluate the binding condition of the Bt toxin and the midgut epithelial cells on the premise of retaining the original structure and physiological environment of the midgut tissue of the larvae, provides direct data support for Bt resistance research, assists in analyzing the pest Bt resistance mechanism, has important significance in formulating resistance early warning and treatment strategies, and has a wide application prospect. The method is suitable for the biotechnical fields of Bt toxin insecticidal mechanism research, lepidoptera pest drug resistance mechanism exploration and the like.

The invention relates to the technical field of preparation of phenylisoxazole derivatives, in particular to phenylisoxazole derivatives containing pyrazole or triazole amide as well as a preparation method and application of the phenylisoxazole derivatives containing pyrazole or triazole amide. The phenylisoxazole derivative containing pyrazole or triazole amide provided by the invention has good insecticidal activity on lepidoptera pests such as plutella xylostella, spodoptera frugiperda, beet armyworm, cotton bollworm and the like, and can be used as a pesticide active component to be applied to prevention and control of agricultural pests and preparation of insecticides.

The invention relates to a botanical insecticidal composition containing 2-tridecanone and Saccharomyces alhagi and application of the botanical insecticidal composition. The botanical insecticidal composition is prepared from 2-tridecanone and Saccharomyces alhagi in a mass ratio of (1: 10)-(3: 1). According to the botanical insecticidal composition provided by the invention, a synergistic interaction effect can be achieved after the 2-tridecanone and the saccharomyces alhagi are mixed according to a specific mass ratio through a large number of experimental research screening, and the botanical insecticidal composition has a better insecticidal effect under the same dosage, so that the compounded composition has relatively high effective activity, and the application prospect is broad. The insecticidal composition can effectively prevent generation of pest resistance or delay the resistance speed, and is lower in cost and smaller in environmental influence. The botanical insecticidal composition provided by the invention has obvious prevention and treatment effects on multiple important lepidoptera pests such as armyworms, plutella xylostella, beet armyworms, cotton bollworms, cabbage caterpillars, prodenia litura and the like.

The application provides a human-derived genetically engineered antibody with targeted simulation of Bt Cry1C toxin structure and insecticidal function and a high-activity mutant of the human-derived genetically engineered antibody, nucleotide sequences and amino acid sequences of the human-derived genetically engineered antibody are respectively shown as SEQ IN NO. 1 and SEQ IN NO. 2, nucleotide sequences and amino acid sequences of the mutant are respectively shown as SEQ IN NO. 3 and SEQ IN NO. 4; after the antibody and the mutant are expressed in prokaryotes, the LD50 of corresponding proteins to Plutella xylostella larvae is respectively 38.9% and 46.8% of the original Bt Cry1C toxin, the LD50 of corresponding proteins to cotton bollworm larvae is respectively 32.9% and 37.4% of the original Bt Cry1C toxin; the two can be respectively used as coating antigens in an IC-ELISA detection method to detect Bt Cry1C toxin with high sensitivity; the antibody and the mutant can be used as antigen analogue materials to replace the structure of Bt Cry1C toxin, not only can be used as new protein insect-resistant materials to have potential application value in green control of agricultural pests, but also can be used for detection of Bt Cry1C toxin residues in the environment and food samples, and have wide application value.