38 results about "Cotton bollworm" patented technology

The invention discloses an insect-resistant transgenic cotton event BrsC35 as well as a detection method and application thereof. According to the invention, an epsps gene, a Cry1Ac gene and a Vip3A gene are transferred into a genome of Zhongmiansui 24 through an agrobacterium-mediated method, so that a transgenic cotton event BrsC35 of which the resistance to lepidoptera insects such as cotton bollworms, ostrinia nubilalis and spodoptera frugiperda is remarkably improved is obtained. The transgenic cotton event BrsC35 is preserved in China General Microbiological Culture Collection Center (CGMCC) on February 17, 2025, and the preservation number is CGMCC No.32181. The transgenic cotton event BrsC35 has been preserved in China General Microbiological Culture Collection Center (CGMCC). The invention also provides a method for identifying whether a plant sample is derived from the transgenic cotton event BrsC35 or an offspring of the transgenic cotton event BrsC35, the transgenic cotton event BrsC35 can be specifically detected, and the transgenic cotton event BrsC35 can be better supervised and managed. The method has an important application value.

The present application belongs to the field of agricultural pest control, and particularly relates to a method for improving the content and insecticidal activity of exogenous insecticidal protein of insect-resistant transgenic cotton. The method is realized by the following steps: maintaining the soil cadmium concentration at 1-5 mg / kg when the cotton is at the three-leaf stage; and maintaining the soil cadmium concentration at 1-4 mg / kg when the cotton is at the five-leaf stage. The method provided by the present application can significantly improve the content and insecticidal effect of exogenous insecticidal protein of insect-resistant transgenic cotton, and enhance the insecticidal activity against cotton bollworms. The method provided by the present application can improve the control effect on pests while controlling cadmium pollution when planting transgenic insect-resistant cotton on land contaminated by a certain concentration of cadmium, and reduce the use of insecticides. Therefore, the present application provides an effective reference for improving the control effect on pests when planting insect-resistant transgenic cotton on land contaminated by cadmium, and has important practical value and significance.

The invention relates to the technical field of agricultural biology, in particular to cotton bollworm salivary protein Ha22, a recombinant vector, recombinant bacteria and application of the cotton bollworm salivary protein Ha22. The invention provides a cotton bollworm salivary protein Ha22. The amino acid sequence of the cotton bollworm salivary protein Ha22 is as shown in SED ID NO. 1. Transient expression of the Ha22 protein on tobacco can cause tobacco cell necrosis, stable overexpression of the Ha22 protein on cotton plants can induce cotton defensive gene response and positively regulate plant insect resistance, and the Ha22 protein has great application value for cotton insect-resistant breeding. In addition, the protein has the active characteristic of an elicitor, so that the purpose of green prevention and control can be achieved by spraying whole protein to plants through in-vitro expression and inducing plant resistance.

This invention discloses aryl heterocyclic compounds containing substituted unsaturated hydrocarbons, their preparation methods, and uses. The compounds have the chemical structure shown in Formula I. The compounds of this invention are used as insecticides, showing particularly good control efficacy against armyworms, diamondback moths, cotton bollworms, corn borers, and rice leaf rollers, and are expected to become novel insecticides.

The invention belongs to the field of biological control, and relates to application and a method of dsRNA of an NRX gene in control of lepidoptera pests. It is found for the first time that the Neurexin-4 gene participates in the growth and development process of the cotton bollworm and the spodoptera frugiperda, the death rate of larvae of the cotton bollworm and the spodoptera frugiperda is remarkably increased through deletion of Neurexin-4 expression, and finally population development is inhibited. The gene can be used for developing RNA biopesticides for preventing and controlling cotton bollworms and spodoptera frugiperda. The invention provides an interference sequence for the cotton bollworm and grassland spodoptera Neurexin-4 gene, the sequence can inhibit the expression of Neurexin-4, and the sequence can be utilized to interfere the expression of the cotton bollworm and grassland spodoptera Neurexin-4 gene and inhibit the growth of the cotton bollworm and grassland spodoptera Neurexin-4 gene.

The application discloses a cotton bollworm nuclear polyhedrosis virus spike period targeted synergistic adjuvant, a pesticide preparation and application, relates to the technical field of agricultural biological pesticides, and the composite adjuvant is prepared from ultraviolet protection and shielding materials, film-forming materials, midgut infection promoters, penetration and spreading agents, system compatible components and carriers.The adjuvant is applied by unmanned aerial vehicle top spraying, and after being compounded with HaNPV, the deposition rate of the virus on the corn tassel can be increased by 3.2 times, the retention amount is increased by 5.62 times, the destruction rate of the midgut peritrophic membrane of the cotton bollworm larvae is more than 90%, and the mortality of the 3rd instar cotton bollworm larvae is increased to 87.72%.The application solves the two technical bottlenecks of the virus pesticide, i.e., 'difficult coverage of the tassel target' and 'low midgut infection efficiency', and provides key technical support for green and precise prevention and control of the cotton bollworm in the corn tassel period.

The invention discloses an insecticidal composition containing entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and relates to the technical field of biological insecticide, the active ingredients of the insecticidal composition are entomopathogenic virus and jasmonic acid methyl ester or jasmonic acid, and the entomopathogenic virus is nuclear polyhedrosis virus of baculoviridae. The weight ratio of the virus to the jasmonic acid methyl ester or jasmonic acid is 1: (0.5-1000), and the insecticidal composition can be prepared into dosage forms such as a suspending agent, water dispersible granules, wettable powder and the like. The invention also provides a method for preventing and treating lepidoptera pests, which comprises the step of applying the entomopathogenic virus and methyl jasmonate or jasmonic acid to target pests or a living environment of the target pests in a combined manner, and the combined application is simultaneous application or successive interval application. The insecticidal composition has a synergistic effect on lepidoptera pests such as cotton bollworms and beet armyworms, can obviously reduce the food intake, accelerate the death of pests and improve the field control effect, and has a wide application prospect.

The application belongs to the technical field of biology, and particularly relates to a dsRNA of a target Helicoverpa armigera HaPburs gene and application thereof in prevention and treatment of the Helicoverpa armigera. HaPburs RNA interference mediated knockdown reduces the transcript level by 60.01%, and induces morphological abnormalities of pheromone glands (PG), which are irregular black protrusions; the silencing HaPburs reduces the main pheromone component Z 11-16: Ald content, while the male insect attraction rate and mating rate, cumulative egg production also significantly decrease, and the transcript level of the sex pheromone biosynthesis related genes HaPKA and HaACC is also significantly reduced. It is shown that HaPburs the genes participate in PG morphogenesis and regulate sex pheromone biosynthesis through a cAMP-PKA-ACC regulatory axis, thereby affecting the reproductive communication of the Helicoverpa armigera. The application provides a novel target and technical scheme for green and efficient prevention and control of the Helicoverpa armigera, and has important research and development value and application prospect.

The application of broccoli genes BoSULTR1;1 and BoSULTR1;2 in improving plant stress resistance belongs to the technical field of plant genetic engineering. In order to solve the technical problem of how to improve the stress resistance of broccoli plants, the application provides the broccoli genes BoSULTR1;1 and BoSULTR1;2, and the application uses an expression vector or a recombinant bacterium overexpressing BoSULTR1;1 and BoSULTR1;2 genes to prepare a transgenic broccoli plant. The stress resistance related detection results prove that the BoSULTR1;1 and BoSULTR1;2 genes have the ability to improve the resistance of the transgenic broccoli plant to cotton bollworm, pathogenic bacteria and drought. The transgenic material obtained by the application has important theoretical significance and practical value for accelerating the breeding process of stress-resistant plants and improving the breeding efficiency.

The invention discloses an RNA biopesticide as well as application and a preparation method thereof. A first aspect of the present application discloses an RNA biopesticide comprising a double-stranded RNA molecule, the glycosyl moiety of at least one nucleotide of the double-stranded RNA molecule having a 2 '-OMe modification. Research results show that when 2 '-OMe-NTPs is doped into the dsRNA, the dsRNA can bear ultraviolet radiation for a long time, and the stability of the dsRNA can be remarkably improved in a high-temperature environment. More importantly, the composition is prevented from being degraded by nuclease in leaf surfaces or lepidoptera pest cotton bollworm intestinal juice environment, and the control effect on cotton aphids and cotton bollworms is remarkably improved. Therefore, the stability of the dsRNA and the RNAi efficiency are improved by modifying the binding site and only depending on the structural change of the dsRNA under the condition of not introducing other materials.

This application belongs to the field of biopesticides and provides the application of HearNPV-miR-hr5b in the control of cotton bollworm. The nucleotide sequence of HearNPV-miR-hr5b is CGGGCAAAUUUCAAAUAAA. HearNPV-miR-hr5b of this application can enhance the replication and accumulation of HearNPV in the host cotton bollworm, accelerate the larval mortality rate, and increase the rate of resistant cotton bollworm control. It can be used to develop HearNPV synergists and has good application prospects in the biological control of cotton bollworm.

The invention relates to an in-situ binding method for evaluating binding of Bt toxin Cry1Ac and midgut epithelial cells of cotton bollworm larvae. The method comprises the following steps: incubating and culturing midgut tissues of larvae of lepidoptera pests and a Bt toxin-containing Sf-900 II cell culture solution, carrying out immunostaining, observing and photographing through a laser confocal microscope, and finally carrying out quantitative processing on a fluorescence image by utilizing ImageJ software so as to calculate an average unit area fluorescence signal value. And further evaluating the difference between the binding amounts of the midgut epithelial cells and the Bt toxins of the larvae of different strains. The method can intuitively and accurately observe and evaluate the binding condition of the Bt toxin and the midgut epithelial cells on the premise of retaining the original structure and physiological environment of the midgut tissue of the larvae, provides direct data support for Bt resistance research, assists in analyzing the pest Bt resistance mechanism, has important significance in formulating resistance early warning and treatment strategies, and has a wide application prospect. The method is suitable for the biotechnical fields of Bt toxin insecticidal mechanism research, lepidoptera pest drug resistance mechanism exploration and the like.

The invention relates to the technical field of preparation of phenylisoxazole derivatives, in particular to phenylisoxazole derivatives containing pyrazole or triazole amide as well as a preparation method and application of the phenylisoxazole derivatives containing pyrazole or triazole amide. The phenylisoxazole derivative containing pyrazole or triazole amide provided by the invention has good insecticidal activity on lepidoptera pests such as plutella xylostella, spodoptera frugiperda, beet armyworm, cotton bollworm and the like, and can be used as a pesticide active component to be applied to prevention and control of agricultural pests and preparation of insecticides.

The invention relates to the field of biological prevention and treatment, in particular to a stomach toxicity insecticidal active polypeptide as well as a preparation method and application thereof, and more particularly relates to the stomach toxicity insecticidal active polypeptide as well as polynucleotide, an expression cassette, a preparation method, a use method and application thereof. The invention relates to a polynucleotide molecule for coding a stomach toxicity insecticidal active polypeptide. The polynucleotide molecule is selected from one of the following nucleic acid molecules: a nucleic acid molecule consisting of a nucleotide sequence selected from SEQ ID NO: 1; nucleic acid molecules encoding a polypeptide consisting of the amino acid sequence of SEQ ID NO: 2. The Pc-1 has wide and efficient insecticidal activity on various lepidoptera pests such as prodenia litura, cotton bollworm and the like, so that the Pc-1 has broad-spectrum application potential, and an important core molecular entity is provided for developing a new generation of efficient, low-residue and good-environmental-compatibility polypeptide biological insecticide.

The present invention relates to the use of fatty acid esters in deterring oviposition by cotton bollworms. In particular, the use of a combination of methyl palmitate, methyl stearate and methyl oleate in deterring oviposition by cotton bollworms.

The invention provides a pest and disease damage accurate monitoring and recognition system, and relates to the field of agricultural intelligent robots, the pest and disease damage accurate monitoring and recognition system comprises a searchlighting recognition system, a walking mechanism, a mechanical arm assembly, an electric water pump, a central control system, a liquid medicine box, a chassis and a sensing system.The specific recognition control method comprises the steps that 1, addition correction is conducted firstly, then binocular stereoscopic vision calibration is conducted, and a calibration result is obtained; 2, monitoring insect pests of the crops in real time by adopting an improved YOLOv8 algorithm, and identifying corresponding types and numbers of the insect pests; 3, acquiring three-dimensional space coordinates of the insect pest image through the insect pest image; and 4, controlling the dosage of the pesticide according to the three-dimensional space coordinates and the corresponding types and quantity of the insect pests. The system can carry out all-weather and all-directional insect pest recognition, is high in detection precision and high in real-time performance, and effectively improves the accuracy and timeliness of cotton bollworm pest prevention and control.

This invention belongs to the field of insecticide ecological safety evaluation technology, and specifically relates to a rapid evaluation method for insecticide safety based on parasitic wasp embryonic development. This invention uses the embryos of the cotton bollworm *Ichthyophthirius multifiliis* as the research object, selecting embryonic development status as the core evaluation indicator. Combining the statistical significance and quantitative change range of this indicator with the control group, a standardized experimental measurement method and a three-level ecological safety classification rule are established. By selecting commonly used agricultural insecticides bromfenac and abamectin, ecological safety evaluations were conducted. Compared with the control group, the number of normal embryos with bromfenac decreased by approximately 17%, classifying it as a medium-risk insecticide; the number of normally developing embryos with abamectin decreased by approximately 23.21%, classifying it as a high-risk insecticide. This application fills the technical gap in the ecological safety evaluation of insecticides at the parasitic wasp embryonic stage, improves the evaluation system for non-target natural enemies, and can be directly applied to pesticide research and development, insecticide screening, and field application guidance, contributing to the green development of agriculture.

The invention belongs to the field of biological medicine, and relates to a dsRNA compound auxiliary agent and a method and application thereof for preparing RNA biopesticide, BioIon18 biological ions (BI18) are used as a main material for loading dsRNA, and the loaded dsRNA shows relatively strong stability in extreme environments such as RNase A and strong ultraviolet, and can effectively prevent degradation of the dsRNA. Meanwhile, cell uptake of dsRNA can be promoted, and the effect of improving the target gene expression inhibition effect in cotton bollworm larvae is achieved. Other aspect designs of the invention include these BI18 at dsRNA drug complexes suitable for use in the field of ecology, as well as methods of inhibiting expression of target genes by administering these dsRNA drugs, e.g., for use in agricultural pest management.

The application belongs to the field of biological pesticides, and provides application of HearNPV-miR-56 in cotton bollworm control, wherein the nucleotide sequence of the HearNPV-miR-56 is CGGUAACAGAAGCGGUCACG. The HearNPV-miR-56 can improve virus replication amount of cotton bollworm nuclear polyhedrosis virus infected larvae, thereby promoting death, and can be used for developing a cotton bollworm nuclear polyhedrosis virus synergist, and has potential application prospect in biological control of cotton bollworm.

The invention relates to the technical field of pesticide chemistry, and discloses an isoxazoline derivative containing a triazole thioether structure and preparation and application of the isoxazoline derivative, three active fragments of isoxazole, triazole and thioether are innovatively subjected to structural splicing synthesis, and the preparation process is clear in step, mild in reaction condition, excellent in intermediate yield and easy to industrially amplify. The insecticidal activity of the derivative on major agricultural pests such as plutella xylostella, spodoptera frugiperda and ostrinia nubilalis reaches 100%, the activity of the derivative is equivalent to that of fluxazole amide, the derivative can prevent and treat various pests such as cotton bollworm, aphid and rice planthopper, and the derivative is not applied in the field, has no drug resistance of the pests, has the advantages of broad spectrum, high efficiency, difficult generation of drug resistance and the like, can be used for preparing insecticides, and has wide application prospects. A brand new solution is provided for green prevention and control of agricultural pests.

The invention belongs to the technical field of biology, and relates to green prevention and control of cotton bollworm damage. The invention provides dsRNA of a HaStonedB gene as well as application and a method of the dsRNA in prevention and treatment of cotton bollworms. An RNAi technology is used for inhibiting a cotton bollworm Stoned B (HaStonedB) gene, and it is found that a good prevention and treatment effect on cotton bollworms can be achieved, growth and development of the cotton bollworms can be inhibited, and the target gene is a potential cotton bollworm RNAi target gene.

The invention belongs to the technical field of biological control of agricultural pests, and particularly relates to a method for comprehensively evaluating the storage quality of low-temperature storage parasitic wasps and application. Through real-time fixed-point dissection, the effective storage starting point of the cotton bollworm odontolimus starts from 24 + / -4 hours of cocooning; establishing a pupal period effective accumulated temperature equation N = 112.46 / (T-7.94), predicting the developmental duration at the temperature of 7.94-25 DEG C, and further adjusting the developmental duration according to field requirements; an equation Y = 0.8611 X-5. 968 for comprehensively evaluating the pest control ability of the low-temperature storage parasitic wasps is established and used for adjusting the field release number of the parasitic wasps during storage at different temperatures so as to guarantee a good field pest control effect; a storage quality standard for comprehensively evaluating the parasitic wasps is established, and the comprehensive storage quality of the parasitic wasps at different temperatures is determined. The result shows that the comprehensive storage quality at 20 DEG C is the highest, the storage time is prolonged, and the biological control progress of lepidoptera noctuidae is promoted.

The invention belongs to the technical field of insect feeding and insect resistance identification, and particularly relates to an inter-field insect medium for cotton bollworms and application. The field insect prevention medium for cotton bollworms comprises the following raw materials: soybean meal, corn flour, wheat germs, wheat bran, beer yeast powder, konjaku flour, cane sugar, Webster salt, multivitamins, ascorbic acid, linoleic acid, potassium sorbate, streptomycin sulfate, erythromycin, methyl p-hydroxybenzoate, blighted grain hull particles and water. The field insect inoculation medium for the cotton bollworms is low in raw material cost, the preparation method is simple and rapid, application is convenient, and manual insect inoculation can be completed without any professional operation. The inter-field insect killing medium provided by the invention fully meets the nutrition required by the growth and development of cotton bollworms, and can improve the inter-field insect killing effect of the cotton bollworms.

The invention discloses double-stranded circular DNA and application thereof in biological prevention and control of cotton bollworm, and relates to the technical field of genetic engineering and agricultural biological prevention and control, the two circular DNA (recombinant plasmids: pBE-cDNA4-lhAChE and pBE-cDNA4-dsAChE) stably generate double-stranded RNA and long hairpin RNA in an insect body and activate RNA interference (RNAi) effect by targeting a key gene of the cotton bollworm, so that the target gene is silenced; and the compound can also have synergistic interaction with viruses (HaNPV), so that the growth and development of cotton bollworms are inhibited. The invention has the advantages of high stability, strong delivery efficiency, environmental friendliness and the like, and is suitable for biological control of cotton bollworm. The technology can reduce the use of chemical pesticides and promote the sustainable development of agriculture.

The invention discloses a method for evaluating the influence of transgenic herbicide-resistant soybeans (XP-2) on the growth and development of cotton bollworms and symbiotic microorganisms. Relates to the technical field of safety evaluation of transgenic insect-resistant plants on non-target organisms, and evaluates the influence of the transgenic herbicide-resistant plants on the growth and development of the non-target organisms and symbiotic microorganisms by measuring the developmental duration, the fecundity and the diversity of intestinal microorganisms of cotton bollworms. XP-2 and non-transgenic soybean (W28) are used as materials, intestinal flora is analyzed by measuring developmental duration, survival rate and reproductive capacity (single female egg laying amount and hatching rate) of cotton bollworms and 16S rRNA sequencing, and ecological safety of the cotton bollworms is determined. A standardized scientific evaluation method is established, the influence of the transgenic herbicide-resistant plant on the growth and development of non-target organisms and symbiotic microorganisms can be more efficiently and accurately evaluated, and scientific data is provided for evaluating the ecological safety of the transgenic herbicide-resistant crop.

The invention belongs to the technical field of biology, and particularly relates to an insect-resistant protein mutant Cry2AhM, a fused insect-resistant protein FP2Ah1Ac, an expression vector and application. The amino acid sequence of the insect-resistant protein mutant Cry2AhM provided by the invention is as shown in SEQ ID No.1, and the insect-resistant protein mutant Cry2AhM has high insecticidal activity on lepidoptera pests; according to the fusion insect-resistant protein FP2Ah1Ac provided by the invention, the mutant and GFM Cry1A protein are subjected to fusion expression, and the resistance of lepidoptera pests to transgenic products of the lepidoptera pests can be reduced, so that the lepidoptera pests are efficiently killed, and normal growth of plants is not influenced; according to the expression vector provided by the invention, the fusion insect-resistant protein FP2Ah1Ac can be highly expressed in a plant body, so that receptor cotton obtains the character of high cotton bollworm resistance, and the fusion insect-resistant protein FP2Ah1Ac has important significance in reducing insect damage, reducing pesticide use, reducing production cost and improving cotton yield.

The present application relates to the technical field of pest control, in particular to a short peptide simulating a Cry1Ac protein anti-insect function epitope and application thereof. The present application takes a cotton bollworm middle intestine cadherin fragment (HaCad-TBR) as a target, and carries out screening on a phage display random 6-peptide library constructed by the laboratory, to obtain a 6-peptide LTETGK capable of combining with the cotton bollworm middle intestine cadherin, i.e. the 6-peptide has the effect of simulating a key anti-insect function epitope of the Cry1Ac protein, and a recombinant insecticidal protein C8CL2 having good insecticidal activity on the cotton bollworm can be obtained by replacing Loop2 of Domain II in the Cry1Ac. The present application expands the diversity of anti-insect materials of the Cry1Ac protein, and provides a potential alternative new material for preventing and controlling target pests, and can be used for the prevention and control of Lepidoptera pests, and provides technical support for the development of Lepidoptera pest control products.

This invention relates to the field of biological control, specifically to a stomach poison insecticidal active polypeptide, its preparation method, and its application; more specifically, to the stomach poison insecticidal active polypeptide, its polynucleotide, expression cassette, preparation method, usage method, and application. A polynucleotide molecule encoding a stomach poison insecticidal active polypeptide is provided, wherein the polynucleotide molecule is selected from one of the following nucleic acid molecules: a nucleic acid molecule composed of a nucleotide sequence selected from SEQ ID NO:1; or a nucleic acid molecule encoding a polypeptide composed of an amino acid sequence selected from SEQ ID NO:2. Pc-1 exhibits broad and efficient insecticidal activity against various lepidopteran pests such as the beet armyworm and the cotton bollworm, indicating its broad-spectrum application potential and providing an important core molecular entity for developing a new generation of highly efficient, low-residue, and environmentally compatible polypeptide-based biological insecticides.

The invention discloses a plant insect-resistant related protein as well as a coding gene and application thereof. According to the invention, the full length of the SlJAIB14 gene is amplified from tomato, and two restriction enzyme cutting sites of BamHI and Sac I are introduced to the 5'and 3 'ends of the gene through primer sequences. The newly synthesized gene is connected with a pYPX158 plant expression vector containing double 35S promoters through a T4 DNA (Deoxyribose Nucleic Acid) ligase, so that a recombinant plasmid pYPX-S1JAIB14 containing a target gene S1JAIB14 is obtained. According to the invention, plasmids are introduced into agrobacterium tumefaciens (GV3101) by using an electric shock method. The SlJAIB14 gene is transformed into tomato by using an agrobacterium-mediated method, and the resistance of the transgenic plant to cotton bollworm is verified through a feeding experiment.

Provided are an amide derivative 5-chloro-N-(2-fluoro-3-(2-iodo-4-(perfluoropropane-2-yl)-6-(trifluoromethyl)phenyl)carbamoyl)phenyl)thiophene-2-carboxamide and the use thereof in killing, preventing and controlling agricultural and forestry pests, particularly pests with chewing mouthparts, such as plutella xylostella, beet armyworm, and cotton bollworm. The compound is safe for honey bees.