9927 results about "Pathogenic bacteria" patented technology

The inventive subject matter relates to the methods for the induction of immunity and prevention of diarrhea resulting from Escherichia coli. The inventive subject matter also relates to the use Escherichia coli adhesins as immunogens and to the construction of conformationally stability and protease resistant Escherichia coli adhesin constructs useful for inducing immunity to Escherichia coli pathogenic bacteria. The methods provide for the induction of B-cell mediated immunity and for the induction of antibody capable of inhibiting the adherence and colonization of Escherichia coli including enterotoxigenic Escherichia coli, to human cells.

Various aspects and embodiments of the invention are directed to methods and compositions for reversing antibiotic resistance or virulence in and/or destroying pathogenic microbial cells such as, for example, pathogenic bacterial cells. The methods include exposing microbial cells to a delivery vehicle with at least one nucleic acid encoding an engineered autonomously distributed circuit that contains a programmable nuclease targeted to one or multiple genes of interest.

A mutant cholera holotoxin featuring a point mutation at amino acid 29 of the A subunit, wherein the glutamic acid residue is replaced by an amino acid other than aspartic acid, is useful as an adjuvant in an antigenic composition to enhance the immune response in a vertebrate host to a selected antigen from a pathogenic bacterium, virus, fungus or parasite. In a particular embodiment, the amino acid 29 is histidine. The mutant cholera holotoxin may contain at least one additional mutation in the A subunit at a position other than amino acid 29. The antigenic composition may include a second adjuvant in addition to the mutant cholera holotoxin.

The present invention generally relates to organic polymers able to participate in an analyte-recognition process, where an analyte facilitates an energy transfer between an energy donor and an energy acceptor. Certain embodiments of the invention make use of fluorescent conjugated polymers, such as poly(phenylene ethynylene)s and other polymers comprising pi-conjugated backbones. For example, one aspect of the invention provides a fluorescent conjugated polymer and an indicator that can interact with each other in the presence of an analyte to produce an emissive signal. In some cases, the interaction may include energy exchange mechanisms, such as Dexter energy transfer or the strong coupling effect. The interaction of the conjugated polymer and the indicator, in some instances, may be facilitated through specific interactions, such as a protein/carbohydrate interaction, a ligand/receptor interaction, etc. Another aspect of the invention provides for the detection of biological entities, for example, pathogenic bacteria such as E. coli, or viruses such as influenza virus. In some cases, biological recognition elements may be used to determine the biological entity, for instance, carbohydrates that can be used to specifically interact with at least part of the biological entity, such as a protein in the cell membrane of a bacterium. Still other aspects of the invention involve articles, devices, and kits using any of the above-described systems.

An ultraviolet area sterilizer or disinfector is incorporated into a building structure where concern exists regarding the presence of pathogenic bacteria on environmental surfaces. Ultraviolet C (UV-C) generators generate UV-C that is directed to architectural partitions of an enclosed area. The architectural partitions reflect UV-C to kill pathogens in the enclosed area. The device transmits a calculated dose of UV-C from a fixture mounted to an architectural partition in the enclosed area. Once an effective cumulative dose of UV-C has been reflected to radiation sensors, as measured by the sensors, the device shuts down.

The present invention relates to promoting whole body health in humans and animals by using topical oral compositions comprising a safe and effective amount of chlorite ion in admixture with a pharmaceutically acceptable carrier, said compositions being effective in controlling bacterial-mediated diseases and conditions present in the oral cavity and inhibiting the spread into the bloodstream of oral pathogenic bacteria and associated bacterial toxins and resultant inflammatory cytokines and mediators. The present invention also encompasses methods of use of these compositions by topically applying to the oral cavity, a safe and effective amount of chlorite ion to promote and/or enhance whole body health in humans and other animals.

The invention relates to a pest-proof disease-resistant organic fertilizer and a preparation method thereof, belonging to the technical field of organic fertilizers. Tobacco waste and oil tea pulp are utilized as main raw materials to develop a method of the pest-proof disease-resistant organic fertilizer. The invention breaks through the limitations of harmful substances and single product effect due to addition of chemical materials in the traditional organic fertilizer. The pest-proof disease-resistant organic fertilizer is prepared from the following raw materials: tobacco waste, oil tea pulp, organic substances, a poising agent and a microbial inoculant. The preparation method of the organic fertilizer comprises the following steps: crushing tobacco straws, tobacco stems, tobacco powder, oil tea pulp and organic substances, spraying the poising agent and microbial inoculant, mixing, carrying out pool stack fermentation and strip stack fermentation, screening and granulating to obtain the insect-proof disease-resistant organic fertilizer. The high-efficiency biological insect-inhibiting factors contained in the organic substances containing massive mycoprotein can effectively inhibit and kill pests and pathogenic bacteria, and solve the problem of environmental pollution caused by the pesticide fertilizer.

The invention relates to a compound biological antiseptic and preservation agent for food. The compound biological antiseptic and preservation agent is prepared by evenly mixing pure biological solid preparations or products such as epsilon-polylysine, nisin, natamycin, lysozyme, bacillus subtilis, ascorbic acid, a toona sinensis leaf extract, a water-soluble resveratrol preparation, tea polyphenol and chitosan. The compound biological antiseptic and preservation agent has a strong inhibition effect on spoilage bacteria and pathogenic bacteria in various foods and good inhibition action on oxidative deterioration of the food. Compared with the existing various preservatives, the compound biological antiseptic and preservation agent has the characteristics of broad antibacterial spectrum, high safety, high efficiency and convenience in use.

The invention relates to a lactobacillus microcapsule, a method for preparing the same, and the use of the same. The lactobacillus microcapsule consists of an outer layer of wall material, a freeze dried protective agent and lactobacilli. The invention also provides the method for preparing the lactobacillus microcapsule and the use of the lactobacillus microcapsule as a feed additive. The lactobacillus microcapsule can effectively protect the lactobacilli in a core material, prolong the survival time of the lactobacilli at room temperature and improve the tolerance of the lactobacilli to the metal ions in the feed; in addition, the lactobacillus microcapsule has good gastric acid resistance and can disintegrate in the intestinal canal quickly to release the lactobacilli, thereby improving the utilization rate of the lactobacilli, balancing the micro-ecological environment in the intestinal canal, suppressing the growth of pathogenic bacteria, protecting the health of the intestinal canals of animals, reducing the incidence rate of the intestinal canals of the animals and the like.

Methods to reduce or inhibit populations of pathogenic bacteria in animals are disclosed. These methods include providing a number of administrations of probiotic microorganism compositions containing one or more strains or species of probiotic microorganisms. Routes of administration, dosage of administration and pathogenic organisms to be reduced or inhibited are also disclosed.

The present invention provides a composition, comprising: greater than about 0.1% by weight hydrogen peroxide; an aromatic acid component; surfactant; optionally, a solvent; and a carrier. The composition of the invention is useful as a disinfecting composition for killing microorganisms such as bacterium (including Mycobacterium), spores and fungi. The composition provides a pathogenic bacteria kill rate of 99.9% in about 30 seconds when bacteria are exposed to the composition and is effective in providing a Mycobacterium kill of 10⁶ with two minutes or less. Moreover, the compositions of the invention are generally more resistant to catalase deactivation than, for example, an aqueous solution of hydrogen peroxide. The concentration of hydrogen peroxide within the composition may range from about 1% by weight to about 7% by weight and the concentration of aromatic acid component may range from about 0.1% by weight to about 5% by weight. The invention also provides a method for disinfection of a substrate utilizing the composition. The composition of the invention may be used in the foregoing method on a medical instrument, such as an endoscope or the like. Applying the compositions to a substrate may be accomplished in any of a variety of application methods such as by roll coating, dipping, spraying, or rotational tumbling. The composition may be applied to the substrate for a period of time ranging from about 30 seconds to about ten minutes. In this aspect, the invention can further comprise drying the substrate after removing the composition.

A method for preserving a food product, such as meat, including the steps of inoculating meat with an effective amount of euhygienic non-pathogenic, non-spoilage bacteria in order to competitively inhibit the growth of undesired pathogenic and spoilage bacteria. Edible films that incorporate and/or cover euhygenic bacteria on the food product are used to ensure competitive inhibition of undesired spoilage and pathogenic bacteria.

The invention relates to bacillus amyloliquefaciens NCPSJ7 and further relates to an application of the strain in treating plant diseases, diseases before and after harvesting fruits and vegetables as well as in preventing food-borne pathogenic bacteria and putrefying bacteria. The strain is preserved in China Center for Type Culture Collection (CCTCC) on March 22, 2013, wherein the preservation number is CCTCC NO: M2013098 and the strain is named as bacillus amyloliquefaciens NCPSJ7. The thallus and fermentation liquor of the strain disclosed by the invention has the effects of treating plant diseases caused by plant pathogenic fungus including antagonistic peach root rotten disease, fusarium wilt of cucumber, botrytis cinerea, jujube anthracnose, pear black spot, pear blue mould, apple brown rot, apple altermaria leaf spot, watermelon fusarium wilt and the like, as well as diseases after harvesting fruits and vegetables and food-borne pathogenic bacteria and putrefying bacteria including antagonistic staphylococcus aureus, salmonella paratyphi A, vibrio parahaemolyticus, yeast and the like; moreover, the bacillus amyloliquefaciens is broad in spectrum and antibacterial and great in potential in developing novel, efficient and natural biological control and biological preservative and fresh-keeping preparation.

A system and method for reducing or preventing bacterial contamination in food includes application of a bacteriophage treatment to any type of food product at any stage of processing the food product. The bacteriophage treatment may also be applied to non-food surfaces and water systems, which may be susceptible to bacterial contamination and subsequent spread of bacteria. The bacteriophage treatment comprises at least one bacteriophage in a concentration sufficient to reduce or prevent bacterial contamination from pathogenic bacteria and/or spoilage bacteria. In some embodiments, the bacteriophage is able to reduce or eliminate bacteria introduced to a food product after the bacteriophage treatment was applied to the food product. In some embodiments, the bacteriophage treatment includes a buffering agent to maintain the bacteriophage at a pH level that sustains the bacteriophage. In some embodiments, the bacteriophage treatment includes a surfactant and/or a thickener to aid in applying the bacteriophage. Additional adjuvants and enhancers may be used in some embodiments to stabilize the bacteriophage or enhance its performance as an antibacterial agent.

The invention relates to microbial organic fertilizer and compound fertilizer, and preparation methods thereof, belonging to the field of fertilizer production. Firstly, chicken manure, grass peat, brown sugar, carbamide, powdered rock phosphate, rice bran or wheat bran and zymocyte are proportionally mixed and then are fermented, and the microbial organic fertilizer can be prepared by adding functional bacteria to the mixture after the fermentation; and inorganic fertilizer, medium trace elements, humic acid, biologic protein and functional bacteria are proportionally added to the prepared microbial organic fertilizer, the obtained mixture is thoroughly stirred and then is processed by granulation, polishing, drying and screening, thereby obtaining the compound fertilizer. The functional bacteria include photosynthetic bacteria, lactic acid bacteria, microzyme, Gram positive actinomycetes, phosphate-solubilizing bacteria, potassium-solubilizing bacteria and nitrogen-fixing bacteria. The fertilizer prepared by the invention can effectively improve the ecology environment for the growth of crop, increase the quality of the crop, inhibit plant diseases and insect pests and kill pathogenic bacteria, and the medium trace elements contained are of many types, are sufficient in quantities and present high fertilizer efficiency; and during application, the fertilizer just needs to be applied for once, thereby saving labor power and causing no environmental pollution.

Disclosed are vaccines and immunogenic compositions which use live attenuated pathogenic bacteria, such as Salmonella, to deliver ectopic antigens to the mucosal immune system of vertebrates. The attenuated pathogenic bacteria are engineered to secrete the antigen into the periplasmic space of the bacteria or into the environment surrounding the bacteria. The vertebrate mounts a Th2-mediated immune response toward the secreted antigen.

A kit for the rapid detection of pathogens in food supplies. The kit includes a microspot device and one or more indicator reagents to be applied to a well of the microspot device. The employed indicator reagent produces a detectable change upon contact with a pathogen of interest. The microspot device is fabricated from a porous membrane, such as filter paper. A substantially continuous boundary composed of a low melting temperature solid is deposited within the porous membrane extending from the top of the membrane to the bottom of the membrane and defines the peripheral sides of the well. Additionally, a barrier is applied to the bottom of the membrane, thus defining the bottom of the well. The kit can further include growth media for enriching the pathogenic bacteria and instructions for use of the kit employing the microspot device and the one or more indicator reagents.

The invention discloses a strain of Streptomyces and application thereof. The biocontrol Streptomyces is blastmycin Streptomyces and its strain number is JZB130180, which is registered in the general microbiological center of China Committee for Culture Collection of Microorganisms with CGMCC No. 13270. The strain had significant antagonistic effect on 14 kinds of plant pathogenic fungi and 6 kinds of plant pathogenic bacteria in agricultural production, and has wide antimicrobial spectrum. The strain produces chitinase, cellulase, protease and selenium, and has the basic characteristics of biocontrol bacteria. The fermentation broth of this strain has strong control effect on tomato gray mold and peach brown rot, and has significant promotion action on the tomato plants, which indicated that the strain had a broad application prospect in the field of biological control of plant diseases.

The invention relates to the field of microbial detection and discloses a method for rapid detection of enterobacter sakazakii. The method comprises the steps of: adding immunized superparamagnetism nanometer magnetic beads into a sample, adding a stabilizer, and incubating for 45-60min at 30-40 DEG C, wherein the magnetic beads are specifically bonded and enriched on microbial food-borne pathogenic bacteria, namely the enterobacter sakazakii, in the sample; and measuring spinning-spinning relaxation time and calculating a value deltaT2 by taking a bacteria-free sample as a blank control. The method disclosed by the invention has high specificity and sensitivity to the detection of the enterobacter sakazakii, is rapid, and is particularly suitable to detection of low-concentration enterobacter sakazakii, especially the enterobacter sakazakii in dairy products; in addition, the method is convenient and simple to operate and has good reliability and low requirement on assorted equipment.

This invention relates to devices and methods for performing active, multi-step molecular and biological sample preparation and diagnostic analyses employing immunochemical techniques. It relates generally to bioparticle separation, bioparticle enrichment, and electric field-mediated immunochemical detection on active electronic matrix devices utilizing AC and DC electric fields. More specifically, the invention relates to devices and methods for sample preparation/manipulation, immunoimmobilization, and immunoassays, all of which can be conducted on one or more active electronic chip devices within a single system. These manipulations are useful in a variety of applications, including, for example, detection of pathogenic bacteria and biological warfare agents, point-of-care diagnostics, food or medical product quality control assays, and other biological assays.

A method for preserving a food product, such as meat, comprising the steps of inoculating meat with an effective amount of euhygienic non-pathogenic, non-spoilage bacteria in order to competitively inhibit the growth of undesired pathogenic and spoilage bacteria. Edible films that incorporate and/or cover euhygenic bacteria on the food product are used to ensure competitive inhibition of undesired spoilage and pathogenic bacteria.

The present invention relates to novel Syn isomers of racemates and optical isomers of 3-(heteroaryl acetamido)-2-oxo-azetidine-1-sulfonic acids of formula (I) and its use in treating the infections caused by gram-negative pathogenic bacteria.

The invention discloses an organic rice cultivation method, which comprises the following four steps of: 1) rice seedling raising, 2) fertilizer application; 3) reasonable close planting and 4) field management. The organic rice cultivation method is characterized in that the step of fertilizer application comprises the following two sub-steps of: 1) preparing and applying composted farmyard manure: evenly spraying microbial manure into farmyard manure, covering the farmyard manure with a plastic film, processing for 21 days to obtain the composted farmyard manure and then applying the composted farmyard manure in a paddy field; and 2) applying microbial manure: applying the microbial manure in the paddy field with water depth being less than 3cm and transplanting rice seedlings 15 days later after the microbial manure is applied. The organic rice cultivation method has the advantages that the heavy-metal ions and the chemical residues thereof in soil can be eliminated; the caking and the desertification of the soil can be gradually eliminated, the content of organic matters is improved, the pH (potential of hydrogen) is regulated, the granular structure is optimized and the breeding of harmful pathogenic bacteria is inhibited; the nutrients are sufficient; and a large quantity of nitrogen elements in air and various intrinsic elements in the soil are fully utilized, and low-carbon, environmental-friendly, sustainable, circular and high-efficiency agricultural economy is formed.

The invention discloses a composite biological soil conditioner and a preparation method of the composite biological soil conditioner. The composite biological soil conditioner comprises the following steps: 1) preparing charcoal; 2) modifying the charcoal; 3) fermenting livestock excrement; 4) preparing an ecological organic fertilizer; 5) compounding, wherein the soil conditioner comprises the following materials in parts by weight: 130-150 parts of modified charcoal, 250-280 parts of the ecological organic fertilizer, 30-35 parts of humic acid, 5-8 parts of probiotics, 3-5 parts of active peptide, 32-38 parts of a water retaining agent, and 190-220 parts of an auxiliary. The composite biological soil conditioner has the beneficial effects that the soil conditioner not only strengthens the water holding capacity of soil, but also improves the soil structure, reduces the bulk density of the soil, increases the porosity and the content of organic matters in the soil, regulates the pH value of the soil, improves the microbial flora of the soil, inhibits the growth and propagation of pathogenic bacteria of a root system, and complexes and adsorbs the heavy metal ions in the soil, and therefore, the composite biological soil conditioner is a safe and efficient composite biological soil conditioner.