2976 results about "Pathogenic bacteria" patented technology

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention relates to the technical field of biology, in particular to a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention discloses a verticillium dahliae virulence gene VdPHO23like. The verticillium dahliae virulence gene VdPHO23like comprises a polynucleotide sequence for coding an amino acid sequence of SEQ ID NO: 3. According to the invention, expression of the gene is inhibited in a targeted manner through an RNA interference mediated gene silencing technology so as to weaken virulence of pathogenic bacteria, and an efficient, specific and environment-friendly comprehensive prevention and control strategy is provided for verticillium wilt of crops such as cotton.

The invention belongs to the technical field of microorganisms, particularly relates to rice endophytic bacillus velezensis AN6 and application thereof, and particularly provides bacillus velezensis AN6 which is classified and named as bacillus velezensis and has the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20231886. The invention provides a novel rice endophytic bacillus velezensis strain AN6, through separation, screening, identification and experimental verification, it is found that the strain shows broad-spectrum resistance to various phytopathogens including rice bacterial leaf blight, rice blast and the like, the strain has the capacity of inhibiting various phytopathogens and has the capacity of adsorbing heavy metal, and the strain has the advantages of being capable of inhibiting various phytopathogens and capable of being used as a novel rice endophytic bacillus velezensis strain AN6. The method is of great significance to biological control of rice diseases and development of green agriculture.

The invention provides a streptomyces, which is named as Streptomyces sp. And is registered and preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 65917. The invention also provides a fermentation broth of the streptomyces, and an ethanol extract and a crude extract of the fermentation broth. The novel streptomyces, the crude extract thereof and the like have broad-spectrum antibacterial activity, have a good antagonistic effect on various pathogenic bacteria such as banana long leaf spot germs and banana colletotrichum gloeosporioides, can enable hyphae of the banana colletotrichum gloeosporioides to shrink, deform and break, inhibit spore germination of the banana colletotrichum gloeosporioides, and have a good anti-bacterial effect on the banana colletotrichum gloeosporioides. According to the present invention, with the application of the bacterial strain, the colletotrichum gloeosporioides spores are shrunk and broken, the growth of the colletotrichum gloeosporioides is inhibited, the peroxidase activity, the polyperoxidase activity, the CAT activity and the like in the banana peel can be improved, and the bacterial strain is the potential biological agent for the prevention and control of the colletotrichum gloeosporioides diseases, and has wide development space and good development and application prospects.

The invention relates to lactobacillus gasseri FMTA1125B with the effect of preventing, relieving or treating vaginitis and application of the lactobacillus gasseri FMTA1125B. The lactobacillus gasseri FMTA1125B is classified and named as lactobacillus gasseri, and the preservation number of the lactobacillus gasseri FMTA1125B is CGMCC No.34292. The lactobacillus gasseri FMTA1125B has the effects of preventing, relieving or treating vaginitis and the application of the lactobacillus gasseri FMTA1125B. The bacterium can produce beneficial components such as lactic acid, gamma-aminobutyric acid and hydrogen peroxide through metabolism so as to regulate the genital tract environment and improve the health degree; and the traditional Chinese medicine composition has relatively good bacteriostasis and sterilization effects on common vaginal pathogenic bacteria such as escherichia coli, staphylococcus aureus, gardnerella vaginalis, candida albicans, neisseria gonorrhoeae and the like. Therefore, the lactobacillus gasseri FMTA1125B strain can be used for preparing a preparation with the effect of improving related genital tract diseases such as vaginitis.

The invention relates to the field of microorganisms, in particular to a mortierella alpina strain MA with a plant growth promoting function and application of the mortierella alpina strain MA. The strain has remarkable nitrogen fixation, siderophore production, auxin (IAA) production and cellulase activity, can inhibit various soil-borne pathogenic bacteria such as phytophthora capsici and fusarium, and remarkably improves the disease resistance and growth performance of crops such as pepper. The strain has various functions and broad-spectrum action, is suitable for replacing part of chemical fertilizers and pesticides in intensive agriculture, and realizes green and efficient planting of crops.

The invention provides lactobacillus crispatus, which is preserved in the general microbiological center of the China Committee for Culture Collection of Microorganisms on February 7, 2023, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No. 26503. The bacillus subtilis can play a relatively strong role in inhibiting pathogenic bacteria such as gardnerella vaginalis, escherichia coli and staphylococcus aureus at the same time, and also has a certain role in inhibiting candida albicans, two-way proteurella and Atortoria vaginalis. Therefore, the lactobacillus crispatus has the potential of maintaining the environment in the vagina to be faintly acid and improving the mucosal immunity and infection resistance of a host, and can be used for preventing and treating vaginal infection and inflammation, and maintaining, improving or recovering the steady state of the environment in the vagina.

The invention provides a primer group for targeted simultaneous detection of multiple bacterial pathogens based on multiple amplicons and application of the primer group, and belongs to the technical field of biological detection of pathogenic bacteria. The primer group for targeted simultaneous detection of multiple bacterial pathogens based on multiple amplicons provided by the invention consists of 196 pairs of primers, and the nucleotide sequences of the primers are shown as SEQ ID NO: 1-392. By using the primer group provided by the invention, multiple pathogens can be rapidly and efficiently detected at the same time, and the primer group has the technical advantages of high targeting, strong specificity and the like.

The invention provides Yunnan golden camellia root endophytic fungi and application thereof, and relates to the technical field of crop disease control. The Yunnan camellia nitidissima root endophytic fungus is Lecanicillium vase JHC098 and is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2025749, and a liquid fermentation liquor crude extract of the Lecanicillium vase can be applied to prevention and treatment of crop diseases. The invention overcomes the defects in the prior art, and the crude extract of the liquid fermentation liquor of the endophytic fungus Lecanicillium viculare JHC098 at the root of Yunnan golden camellia can effectively inhibit the growth of pathogenic bacteria such as citrus canker and tobacco bacterial wilt, thereby achieving the purpose of certain biological control.

The invention relates to the technical field of microorganisms, in particular to talaromyces purpurogenus, strain fermentation liquor of the talaromyces purpurogenus and application of the talaromyces purpurogenus to prevention and treatment of northern corn leaf blight. The invention provides biocontrol fungus fermentation liquor for preventing and treating northern corn leaf blight and application of the biocontrol fungus fermentation liquor. The preservation number of a strain of the biocontrol fungus fermentation liquor is CGMCC No.41911. The strain and the fermentation liquor thereof can completely inhibit various pathogenic bacteria, active substances in the fermentation liquor are relatively stable, the active validity period is long, the systemic property is relatively strong, the strain is not easy to degrade, high temperature resistance and microwave radiation resistance are realized, the antibacterial effect of chemical agents can be remarkably improved, and the effects of pesticide reduction and synergism are remarkably achieved when the strain and the fermentation liquor are mixed with chemical pesticides; therefore, the environmental safety problem and the drug resistance caused by chemical pesticides are obviously reduced.

The invention discloses talaromyces sp. DFHJ2 and application of a fermentation product of the talaromyces sp. DFHJ2 in strawberry growth promotion and pathogenic bacterium inhibition, and relates to the technical field of microbiology. The talarospora scabra is preserved in the China General Microbiological Culture Collection Center on February 17, 2025, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.41813. The talarospora scabra is named as talarospora scabra. According to the invention, a talaromyces scabra strain is separated from the root of 'Zhongjiang Junjun' strawberry, and the talaromyces scabra strain has a good antagonistic effect on strawberry anthracnose and other types of strawberry pathogenic bacteria which are seriously harmful in the current production, and can be used for efficiently preventing and treating strawberry anthracnose and other strawberry diseases. In addition, the strain is moderate in growth speed, large in sporulation quantity, easy to ferment and high in adaptability to the pH value of the environment, has a certain growth promoting effect on strawberry seedlings, and has the huge potential of being developed into an efficient biocontrol microbial agent.

The invention relates to a bifidobacterium animalis subsp. Lactis YYSJ001 with a blood sugar reducing effect and application of the bifidobacterium animalis subsp. Lactis YYSJ001. The classification name of the strain is bifidobacterium animalis subsp. Lactis, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.33471. The invention further relates to a preparation method of the bifidobacterium animalis subsp. Lactis YYSJ001. The bacterial strain has good artificial gastric juice resistance, artificial intestinal juice resistance and intestinal tract adhesion ability, has an inhibition effect on numerous pathogenic bacteria such as escherichia coli, staphylococcus aureus, salmonella, klebsiella pneumoniae and quail chicken enterococcus, and can stimulate intestinal cells to generate glucagon-like peptide-1, so that the bacterial strain can be applied to preparation of various pathogenic bacteria. Diseases of diabetic mice can be effectively improved, and the compound can be used for preparing products for preventing and treating diabetes or assisting in reducing blood sugar.

The invention discloses a pseudomonas synthetic flora and application thereof in prevention and treatment of apple continuous cropping obstacles, and belongs to the technical field of agricultural microorganisms. The synthetic flora is constructed from three strains of pseudomonas, i.e., alkylphenol pseudomonas, oleaginous pseudomonas and pseudomonas putida, and the biological preservation numbers of the three strains of pseudomonas are respectively as follows: alkylphenol pseudomonas S4, CGMCC (China General Microbiological Culture Collection Center) No.36078; the culture medium is characterized in that the culture medium is Pseudomonas oleovorans S38, and the culture medium is CGMCC (China General Microbiological Culture Collection Center) No.36079; pseudomonas putida S42, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.36080, is CGMCC No.36080. The synthetic flora has a relatively strong antagonistic effect on main pathogenic bacteria (fusarium oxysporum and fusarium solani) of apple continuous cropping obstacles, and the inhibition capability of the synthetic flora is remarkably superior to that of a single strain; field and pot experiment results show that the liquid bacterial fertilizer and the solid bacterial fertilizer of the synthetic flora can promote the growth of overground parts and underground parts of the continuous cropping apple trees; the number of harmful fungi in continuous cropping soil is obviously reduced; the root protecting enzyme activity is improved, and the MDA content is reduced; the rhizosphere micro-ecology of the continuous cropping apples is optimized, and the continuous cropping obstacles of the apples are remarkably relieved.

The invention discloses a broad-spectrum lytic bacteriophage for preventing and controlling various vibrio infections in an aquaculture environment. The invention relates to V.alginolyticus phage vBValM-R37J, which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on August 6, 2025, the address is No.100 Courtyard, Xianlie Middle Road, Xianlie District, Guangzhou City, Guangdong Province, the postcode is 510070, and the preservation number is GDMCC NO.66813-B1. The invention further discloses a method for preparing the V.alginolyticus phage vBValM-R37J strain. The bacteriophage R37J is a candidate therapeutic bacteriophage with wide host spectrum, high splitting efficiency, gene safety and environmental stability, and has the potential of being popularized and applied in aquaculture as a novel biological tool for accurately preventing and controlling various vibrio infections. A new biological resource is provided for accurate prevention and control of aquatic pathogenic bacteria, and a theoretical basis and a technical support are provided for popularization and application of a phage therapy in an actual culture system.

The invention relates to application of turmeric rhizome extract emulsifiable concentrate in preventing and treating corn leaf spot and tomato gray mold. Compared with the prior art, the turmeric rhizome extract missible oil disclosed by the invention contains an effective substance aromatic turmerone, and the aromatic turmerone can reduce the spore germination rate and sporulation quantity of pathogenic bacteria, increase the permeability of hypha cell membranes and cause overflow of contents, so that normal growth of hyphae is inhibited, and an antibacterial effect is achieved. Aromatic turmerone acts on plants, so that plant cells generate more polyphenol and flavonoid substances, and the immunity of the plants is improved. Pharmacodynamic test results in fields in two fields show that the 6.5% turmeric rhizome extract emulsifiable concentrate can be used for stably and effectively preventing and treating corn northern leaf blight and tomato gray mold after being diluted by 150-300 times, and also has a yield increasing effect.

The invention discloses a Burkholderia sp. Strain and an application thereof in prevention and treatment of eucalyptus blight. The Burkholderia sp. AS-SF-1 disclosed by the invention has the preservation number of GDMCC (Graphical December Microorganism Center) No. 66223; the culture and volatile matter of the strain can inhibit growth of various forest pathogenic bacteria, the eucalyptus has a remarkable control effect on branch blight, siderophore can be generated, absorption and utilization of iron can be promoted, various types of carbon sources or nitrogen sources can be utilized, and the strain can grow within the pH range of 4-9. The burkholderia sp. AS-SF-1 disclosed by the invention has an important application value on planting of eucalyptus and prevention of diseases.

The invention relates to a microbial agent prepared based on a new strain of bacillus velezensis, which has the function of broad-spectrum antagonism of common pathogenic bacteria of aquatic products and can effectively prevent and / or treat bacterial diseases and / or fungal diseases of aquatic organisms including crustaceans and fishes. Meanwhile, the content of nitrite and ammonia nitrogen in aquaculture water can be effectively reduced, and the usage amount and residual quantity of antibiotics and chemical drugs in aquaculture are reduced.

The invention discloses a Trichoderma auritum GUWM22 strain and application thereof, and belongs to the field of microorganisms. The preservation number of the trichoderma auritum GUWM22 is CCTCC (China Center for Type Culture Collection) NO: M20242639, the preservation date is November 26, 2024, the preservation unit is China Center for Type Culture Collection, and the preservation address is Wuhan University, Wuhan, China. Experiments prove that the GUWM22 has a very strong inhibition effect on six pathogenic bacteria (phytophthora nicotianae, phytophthora capsici, botryosphaeria dothidea, alternaria alternata, phomopsis and colletotrichum capsici), the bacteriostasis rate reaches 78% or above, in addition, the GUWM22 also has a good prevention and treatment effect on anthracnose of picked peppers (line peppers), the prevention effect is 82.11%, and it can be seen that the GUWM22 can be used for preventing and treating anthracnose of the picked peppers (line peppers). The Trichoderma aureum GUWM22 is a new biocontrol Trichoderma strain with good potential. The invention provides a new strain resource and an environment-friendly and efficient biocontrol approach for effective prevention and treatment of pepper anthracnose.

The invention relates to a bacterial pneumonia pathogenic bacterium identification method based on an intelligent Raman technology, which comprises the following steps: S1, setting a type label according to a gram staining characteristic classification mode or the occurrence frequency of bacterial types in bacterial pneumonia infection, and selecting a known strain according to the type label, collecting Raman spectrum data of a known strain; s2, carrying out wave number extraction, background subtraction, smooth noise reduction and normalization preprocessing on the Raman spectrum data; s3, constructing a classification model according to an AlexNet model; s4, dividing the preprocessed Raman spectrum data into a training set and a verification set, and inputting the training set, the verification set and corresponding type labels into a classification model for training to obtain a trained classification model; and S5, acquiring Raman spectrum data of detected bacteria by using a Raman spectrometer, preprocessing the Raman spectrum data, and inputting the preprocessed Raman spectrum data into the trained classification model to obtain the type of the detected bacteria. The bacteria are identified by establishing the classification model, so that the operation complexity is reduced, and the identification time is shortened.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to Lecanicillium lecanii as well as a fungicide and application thereof, Lecanicillium lecanii GC-2 is obtained by screening and separating phyllospheric microorganisms, and the strain is proved to have relatively strong inhibitory activity on wheat stripe rust, fusarium graminearum and pseudocereal fusarium; a pot experiment verifies that the strain has a very strong inhibition effect on uredospores of wheat stripe rust, has a relatively strong antagonism effect on pathogenic bacteria of wheat scab and wheat stem rot, has a remarkable prevention and treatment effect on the wheat stripe rust, the wheat stem rot and the wheat scab, has a better prevention effect than a treatment effect, and can be widely applied to prevention and treatment of wheat stripe rust, wheat stem rot and wheat scab. Meanwhile, the strain has no adverse effect on growth and development of wheat and can be applied to prevention and treatment of wheat stripe rust, basal stem rot and gibberellic disease. The invention provides a new strain resource for a biocontrol resource library of plant diseases.

The invention belongs to the technical field of biology, and discloses application of a mycoplasma hyopneumoniae Ebh GA protein in preparation of mycoplasma hyopneumoniae or a multi-combined vaccine containing the mycoplasma hyopneumoniae. The applicant screens out the Ebh GA protein with immunogenicity from the mycoplasma hyopneumoniae for the first time, the Ebh GA protein is shown in SEQ ID NO.7 and can be used as a mycoplasma hyopneumoniae subunit vaccine, and therefore the applicant screens out the protein with immunogenicity of other pathogenic bacteria at the same time and combines the protein with the protein into a quadruple subunit vaccine. The antigens of the obtained quadruple vaccine have a synergistic effect, and compared with a single dose, the quadruple vaccine can enhance the immune effect of the quadruple vaccine.

The invention relates to the technical field of public health safety, and discloses a quantitative evaluation method and system for pathogen transmission risk, and the evaluation method comprises the following steps: step 1, obtaining virulence genes, reference virulence genes, environmental parameters, transmission factors and host risk factor parameters of pathogen samples; 2, performing variation detection on the virulence gene by adopting various variation detection algorithms to obtain all variation sites, and processing all variation sites to obtain effective variation sites; 3, constructing a virulence index model, inputting the variation distance into the virulence index model, and calculating the virulence index of the pathogenic bacteria sample; and 4, constructing a propagation risk assessment model, inputting the virulence index, the environmental factor, the propagation coefficient and the host susceptibility risk index into the propagation risk assessment model, and outputting a risk value by the propagation risk assessment model. According to the method, accurate prediction of the pathogen transmission risk can be realized.

The invention relates to the technical field of bacterium detection, in particular to a colorimetric-Raman coding immunochromatography test strip based on a 3D film-shaped SERS probe and a multi-bacterium detection method thereof. According to the technical scheme, the kit comprises a sample pad, a nitrocellulose membrane, a water absorption pad, a 3D membrane-like SERS probe and an operation buffer solution. According to the present invention, the immunochromatography of the 3D film-like probe is provided, the MoDAu-coated Ag SERS nano-film is adopted as the probe in the system, such that the pathogenic bacteria can be highly specifically recognized and captured, the immune complex fluidity can be increased, the rapid quantitative detection of the three pathogenic bacteria can be achieved, and the flexible 3D film-like SERS probe capable of enhancing the colorimetric ability and the SERS activity and highly specifically capturing the pathogenic bacteria can be prepared; by combining with an antibody-modified ICA test strip, rapid, simultaneous and quantitative determination of various pathogenic bacteria is realized.

The invention relates to the technical field of high-throughput targeted sequencing, in particular to a primer group for detecting important drug-resistant genes and virulence genes of multi-drug-resistant pathogenic bacteria and application of the primer group. Specifically, the kit comprises detection primers for multiple drug-resistant bacteria such as third-generation cephalosporin drug-resistant enterobacteriaceae bacteria, carbapenem drug-resistant enterobacteriaceae bacteria, carbapenem drug-resistant acinetobacter baumannii, rifampicin drug-resistant mycobacterium tuberculosis and the like. The method has the technical advantages of rapidness, high efficiency, strong targeting property, strong specificity and the like, is suitable for epidemic and difference analysis of various important drug-resistant genes and virulence genes carried by metagenome samples of various environments or disease materials and the like, evaluates the risk of related environments on the basis, and can further guide prevention and clinical treatment medication.

Provided are a method for controlling a soilborne disease in which a load on an environment and a human body is reduced, and a novel predatory bacterium therefor.　Provided are: a predatory bacterium or a variant thereof selected from the group consisting of bacteria of the genus Bacteriovorax and bacteria of the genus Bdellovibrio which selectively prey on a pathogenic bacterium belonging to the family Burkholderiaceae, the family Pectobacteriaceae, or the family Lysobacteraceae; a method for controlling a soilborne plant disease caused by the pathogenic bacterium, the method characterized in that the predatory bacterium or variant thereof is applied to a seed, root, or underground stem of a plant, rhizosphere soil, or soil; and a composition.

The invention relates to the technical field of biological medicine, and discloses a formula of compound probiotic enzyme powder for promoting gastrointestinal microecological balance and nutrient absorption, by constructing a cross feeding system of bifidobacteria, lactobacillus and butyric acid bacteria and combining a stomach-intestine stage enzyme activity gradient adaptation strategy and a microenvironment response type double-layer embedding technology, the gastrointestinal microecological balance and nutrient absorption can be promoted, and the gastrointestinal microecological balance and nutrient absorption can be promoted. And cross-scale synergy of flora metabolism, substrate conversion and targeted release is realized. Wherein the sodium alginate-pectin composite embedding structure forms a pH buffer micro-area in a gastric acid environment, and proton pump gene expression of the acid-resistant strain is synchronously triggered; the prebiotics compounding and step-by-step enzymolysis process breaks through a closed-loop path of fiber degradation-flora proliferation-short-chain fatty acid generation. Compared with a traditional single-strain intervention scheme, the method has the advantages that double limitations of flora function splitting and process isolation are broken through, and systematic technical advantages are formed in the aspects of improving flora colonization stability, enhancing metabolite conversion efficiency and inhibiting pathogenic bacterium colonization.

The invention relates to the technical field of microorganisms, and particularly discloses bacillus velezensis DSSR-2, a biocontrol microbial agent and application of the bacillus velezensis DSSR-2, the bacillus velezensis DSSR-2 is preserved in the China General Microbiological Culture Collection Center on July 8, 2024, the preservation number is CGMCC No.31207, and the bacillus velezensis DSSR-2 is classified and named as bacillus velezensis. The bacillus velezensis DSSR-2 provided by the invention has broad-spectrum antibacterial activity and can be used for inhibiting pathogenic bacteria of plant diseases, so that the plant diseases are prevented and treated, and meanwhile, the growth and development of plants are promoted.

The invention discloses a primer probe group and a kit for detecting southern rust pathogenic bacteria, namely puccinia polypoda, based on MIRA-LFIA and application of the primer probe group and the kit. The primer probe group comprises a forward primer, a reverse primer and a probe combination, and the nucleotide sequence of the forward primer is as shown in SEQ ID NO.1; the nucleotide sequence of the reverse primer is as shown in SEQ ID NO. 2; the nucleic acid sequence of the probe primer is as shown in SEQ ID NO. 3. The MIRA-LFIA detection method of the pathogenic bacteria is established on the basis of the primers, and the detection method has good specificity and sensitivity, short detection time and low cost, realizes visualization of interpretation results by combining lateral flow immunochromatography color development, and is especially suitable for field real-time monitoring. The detection method provided by the invention lays a foundation for early diagnosis of puccinia multiflora and development of a monitoring technology of pathogenic bacteria, and provides guarantee for high yield of corn.

The invention discloses a Brucella with antibacterial activity and application of the Brucella. The invention discloses Burkholderia sp.HNIV0701, and the preservation number of the Burkholderia sp.HNIV0701 is GDMCC No: 66007. The Burkholderia sp.HNIV0701 disclosed by the invention has a stable, efficient and broad-spectrum antibacterial property, has an inhibition effect on a plurality of plant pathogenic bacteria such as Botryosphaeria dothidea, Phytophthora nicotianae, Fusarium solani, Alternaria alternata, Thanatepora cucurbitae, Ralstonia solanacearum and the like, and also can be used for inhibiting Staphylococcus aureus and / or Listeria monocytogenes, so that the Burkholderia sp.HNIV0701 can be used for inhibiting a plurality of plant pathogenic bacteria such as the Botryosphaeria dothidea, the Phytophthora nicotianae, the Fusarium solanum, the Alternaria solanacearum and the like. The Brucella HNIV0701 can hydrolyze organic phosphorus and can be used for soil improvement. The Brucella HNIV0701 has nitrogen fixation capability and has the potential of promoting plant growth.

The invention belongs to the technical field of antibacterial active microorganisms, and particularly relates to a strain of diffusion carbon pad bacteria LUCF 4343-3, a bacterial agent and application of the bacterial agent in preparation of an antibacterial preparation. According to the invention, an endophytic fungus strain with remarkable antibacterial potential is successfully separated from Parmotrema, and the endophytic fungus strain is identified as diffusion carbon pad fungus LUCF 4343-3. Experiments show that an ethyl acetate extract of the strain shows a good inhibition effect on various clinical common pathogenic bacteria, including gram-positive bacteria (staphylococcus aureus and enterococcus faecalis), gram-negative bacteria (pseudomonas aeruginosa), fungi (candida albicans and candida glabrata) and the like; the discovery provides an important basis for the application of the strain in the development of novel broad-spectrum antibacterial drugs.