385results about How to "Proliferation effect is good" patented technology

The invention relates to a ginseng adventitious root induced proliferation method, which comprises the following steps: cutting ginseng tissue culture seedlings into small tissue blocks and then inoculating into a solid induced culture medium for inducing to form adventitious roots, cutting the adventitious roots into small adventitious root blocks and then inoculating into a liquid proliferation culture medium for performing proliferation culture of the adventitious roots, wherein the solid induced culture medium and the liquid proliferation culture medium take 1 / 2 MS(-N) culture medium as basic culture mediums and contain 1-10mg / L indolebutyric acid. By utilizing the method provided by the invention, the quantity of the adventitious roots obtained through induction can be obviously increased, the adventitious roots obtained through induction are subjected to proliferation culture, and compared with the method for performing adventitious root induction by utilizing callus tissues, the proliferation rate of the adventitious roots is higher, and the proliferation effect is better.

The invention relates to a CD47 and PD-L1 targeting bifunctional fusion protein, belongs to the field of biomedicine, and solves the problems that anti-PD-1 / PD-L1 treatment is poor in a low-immunogenicity tumor treatment effect and anti-CD47 treatment is poor in a targeting ability. The fusion protein is composed of a CD47 binding part and a PD-L1 binding part which are linked by a disulfide bond, can block the binding of CD47 and SIRPalpha, can block the binding of PD-L1 and PD-1, can achieve the effects of activating macrophages to phagocytize tumor cells and promoting antigen presentation in natural immunity, can achieve an effect of promoting the activation of tumor specific T cells in acquired immunity, and has lower blood toxicity; and as compared with the single use of anti-PD-L1 or anti-CD47 for treatment, the fusion protein has better antitumor curative effects and blood safety.

The invention discloses a production method of a high-salt diluted and low-temperature fragrant soy sauce, which comprises the following steps of: (1) curing raw materials including wheat and defatted soybeans, and then uniformly mixing the wheat and the defatted soybeans; (2) inoculating aspergillus oryzae strains to the obtained raw material mixture so as to obtain a yeast; (3) cultivating the yeast for 44-48 hours at a temperature of 25-30 DEG C so as to obtain a mature yeast; (4) adding 2.0-2.5 weight times of saline water into the mature yeast so as to obtain a raw soy sauce mash; (5) moving the raw soy sauce mash to the inside of an airtight fermentation tank for fermenting: firstly fermenting for 23-27 days at a temperature of 13-17 DEG C, then continuing to ferment at a temperature of 28-32 DEG C, and on the 28-32nd days, adding mixed fermentation strains into the fermentation tank and continuing to ferment; in the process of fermentation, stirring by using a compressed air flow; and fermenting for more than six months so as to obtain a mature soy sauce mash; and (6) squeezing and filtering the mature soy sauce mash so as to obtain the low-temperature fragrant soy sauce. According to the invention, the conversion rate of proteins in raw materials is high, and the contents of ammonia nitrogen, total nitrogen, soybean peptides and soybean oligosaccharides in the prepared soy sauce are high.

The invention relates to an industrial aquaculture fish shelter. The industrial aquaculture fish shelter is characterized in that a first fish shelter body is composed of a cover plate and a base, a gas-filling device is arranged on the cover plate, and the gas-filling device which is located in a cavity is connected with a gas bag device; the base is provided with a plurality of through holes, and a plurality of concave pits are formed in the outer surface of the cover plate; an arc-shaped panel body of a second fish shelter body is arranged on the outer side of a star-like structure and partially covers the star-like structure, and the arc-shaped panel body and the star-like structure define one or more containing bodies with the two ends opened; an anchor body of a positioning anchor is provided with a plurality of containing cavities, barbs are arranged in the containing cavities, and the barbs are in rotation connection with a convex block on the anchor body through pin shafts. According to the industrial aquaculture fish shelter, the first fish shelter body can be matched with the positioning anchor to control the suspension height of the fish shelter by inflating and deflating the gas bag, and the second fish shelter body is of a porous structure and has the advantages of being large in concealed space, high in concealing property, good in water body circulation and the like; the positioning anchor is matched with the first fish shelter body and can regulate the suspension depth to conduct artificial enhancement, and the enhancement effect is good.

The invention relates to a preparation method of a magnesium alloy / hydroxyapatite composite, which comprises the steps of firstly preparing nano hydroxyapatite powder by a sol-gel process, and then uniformly mixing the magnesium powder with the nano hydroxyapatite powder; putting the mixture into a mould and performing cold pressing; putting the cold-pressing formed sample into a vacuum heat treatment furnace for sintering to obtain the magnesium alloy / hydroxyapatite composite. Compared with the prior art, in the method provided by the invention, the magnesium alloy is used as a metal matrix, the hydroxyapatite with the same chemical composition as the human skeleton and having low solubility in the human body environment is used as a reinforcing body, and the method aims at preparing a magnesium alloy / calcium phosphate composite biomedical material by use of a powder metallurgical process; the material is mainly applied to clinical medicine as a degradable endosteal fixing material, a porous bone repair material, a dental implantation material, an oral repair material, a cardiovascular stent and the like, and has broad prospects in terms of bone tissue defect repair.

The present invention provides one kind of slow-releasing basic fibroblast growdth factor (bFGF) microsphere and its preparation and use. The microsphere has coated bFGF as medicine, and matrix of polylactide-co-glycolide (PLGa). The slow-releasing bFGF microsphere is prepared by using PVA or mixed PVA-PEG liquid as dispersing medium and through w / o / wt% re-emulsion and drying process and mechanical stirring. Its freeze dried power has good dispersivity and low organic solvent residue. The present invention can be used for local administration or vein administration of treating fracture, bone defect and other diseases.

The present invention relates to an application of iridoid in the preparation of anti-osteoporosis medicines, which belongs to the medicines technical field. The invention provides an application of iridoid which is shown in a general formula I in the preparation of medicines used for treating and / or preventing osteoporosis. Experiments found that ALP activity in UMR106 cells is taken as guidance for tracing and separating an extract of iridoid possessing general formula I, the extract and each separated component are researched from the aspects of osteoblast propagation and antioxidation activity, the iridoid possessing the general formula I has the effects of substantially promoting alkaline phosphatase activity and osteoblast differentiation, so that the iridoid has anti-osteoporosis effect and can be used for preparing the medicines used for treating or preventing osteoporosis. The component of specnuezhenide and a compound G13 possesses the effects of substantially promoting the alkaline phosphatase activity and osteoblast differentiation, and the specnuezhenide has effects for obviously promoting UMR-106 cell proliferation, a better effect is obtained when the specnuezhenide is used for anti-osteoporosis effect.

In the present invention the microsphere has coated bFGF as medicine, and matrix of lactic acid-glycol copolymer (PLA-PEG). The slow-releasing bFGF microsphere is prepared by using PVA-PEG mixed liquid as dispersing medium and through evaporation and mechanical stirring. It has excellent slow-releasing performance of over two-week releasing period. Its freeze dried powder has good dispersivity and low organic solvent residue. The present invention can be used for local administration or vein administration of treating fracture, bone defect and other diseases.

The invention provides an undifferentiated anti-aging amplification culture medium for human umbilical cord / adipose tissue-derived stem cells. The problem in the prior art that a serum-free medium used for culturing mesenchymal stem cells is easy to differentiate and easily causes aging and apoptosis of adult stem cells is solved. The undifferentiated anti-aging amplification culture medium for human umbilical cord / adipose tissue-derived stem cells comprises cell factors, vitamins and chemical small molecules, and the culture system comprises multiple chemical small molecules and biomacromolecules and has the effects of improving physiological metabolism and proliferation and growth potential of cells, resisting oxidative stress response, scavenging free radicals, preventing DNA mutation, protecting mitochondria, reducing molecular wastes in the cells and activating longevity genes and telomerase.

The invention discloses a method for building a leaf in vitro regeneration system of begonia rex. The method comprises the following steps of: (1) selecting an explant and sterilizing the explant; (2) carrying out adventitious bud induced cultivation after cutting the processed leaves; (3) carrying out high-voltage electrostatic field treatment; (4) carrying out subculture proliferation cultivation on the adventitious bud induced by the leaves; (5) carrying out rooting culturing on a test-tube plantlet after the subculture proliferation cultivation; and (6) cutting and rooting. The begonia rex is subjected to tissue culturing by adopting the propagation method; the reproduction rate of a culturing period (51d) is 243.1 times; the propagation speed is fast; and the problem of seeding propagation of the begonia rex is effectively solved.

The invention provides a method for promoting growth of probiotics by adding traditional Chinese medicine components into a grain culture medium. The method comprises the following steps: inoculating the probiotics into an improved MRS (Methicillin-Resistant Staphylococcus) lactobacillus culture medium or a mixed grain puffed powder culture medium added with different traditional Chinese medicine components and fermenting. Each liter of the culture medium contains 5g of mulberry leaf extract or cordyceps polysaccharide or a mixture of a plurality of traditional Chinese medicines. The probiotics are lactobacillus rhamnosus or a plurality of types of mixed probiotics. According to the method provided by the invention, the fermentation speed of the probiotics in a fermentation solution and the active bacteria number of the probiotics in the fermentation solution can be remarkably improved; the mulberry leaf extract or the cordyceps polysaccharide is added; the culture medium is fermented for 12h and the active bacteria number reaches 10<9>CFU / mL; after the grain culture medium added with the traditional Chinese medicine components is fermented, the active bacteria number reaches 4.1*10<8>CFU / mL. Meanwhile, an experiment method for detecting the active bacteria number of the probiotics in a turbid fermentation system by adopting flow cytometer detection is established.

The invention relates to a separation and efficient amplification culture method for an antigen specific T lymphocyte. By means of the immunomagnetic bead technology and the character of an active T cell expression CD137, the antigen specific T lymphocyte is separated from a complex cell mass; then an antigen specific T lymphocyte with high tumor killing activity is obtained through in-vitro efficient amplification culture. CD137L (CD137-ligand), IL-7, IL-15 and IL-2 are added in the culturing process, so that the phenomenon that activated and induced cells are liable to apoptosis in the culturing process is avoided, the amplification time is increased, and the killing activity of the antigen specific T lymphocyte is improved.

The invention provides serum-free culture fluid for culture of salivary gland epidermal cells and salivary gland stem cells of mammals, which is prepared by using MCDB153HAA as basic culture medium and adding amino acid, vitamins, salts, lipid, trace elements, buffer fluid, hormone-like compounds, transgenic metalloprotein, antioxidants, seralbumin, glucide, purine, pyrimidine base substances and pH value indicators. The serum-free culture fluid has the advantages that salivary gland epidermal cells of the mammals can vigorously grow, good cell activity and physiological properties are maintained, in addition, the serum-free culture fluid is also suitable for the salivary gland epidermal cell culture, is particularly suitable for the field of study relevant to biological tissue engineering and belongs to the commercial cell culture fluid.

A lactic acid bacteria fermented substance is characterized by being obtained by culturing lactic acid bacteria in a medium containing an extract of at least one food material selected from the group consisting of rice bran, persimmon leaf, Perilla frutescens, Houttuynia cordata, Eucommia ulmoides, turmeric, clove, cinnamon and Rubus suavissimus. Because the extract to be used in the production of the lactic acid bacteria fermented substance does not cause a flavor problem and can increase the number of living lactic acid bacteria easily by simply being added to and mixed in the medium, the lactic acid bacteria fermented substance containing many living lactic acid bacteria in which high activity is maintained and a food or a drink using the same can be obtained.

The embodiments of the invention disclose a solid beverage with an intestinal tract regulating function. The solid beverage is prepared from the following raw materials in percentage by weight: 54% to75% of complex prebiotics, 10% to 35% of dietary fibers, 2% to 10% of integrated fruit and vegetable fermented powder and 2% to 10% of pea peptide, wherein the complex prebiotics comprise dahlin, lactitol, oligomerized fructose, xylo-oligosaccharide and stachyose, and the dietary fibers comprise resistant dextrin and psyllium seed husks. According to the solid beverage, the kind of and proportioning ratio of each raw material of the solid beverage are extensively researched, so that the final obtained finished product is beneficial to improvement on health of intestinal tracts, can be used for effectively preventing diarrhea and loosening bowels to relieve constipation and achieves the action of improving constipation. The composition disclosed by the invention is natural and is free of side effect, safe and effective in use and broad in market application prospect.

The invention discloses a preparation method of xylooligosaccharide with low DP (degree of polymerization). The preparation method is characterized by comprising steps as follows: (1) wood fiber biomass is taken as a raw material and subjected to diluted acid / diluted base / hot water pretreatment, a liquid is filtered, a solid phase is treated in a high-temperature degrading or steam explosion pretreatment manner, and the wood fiber biomass is cooled for later use; (2) xylanase is added to a pretreatment solution in step (1) for an enzymatic hydrolysis reaction; (3) an enzymatic hydrolysis solution is treated through enzyme deactivation, purification, concentration and drying, and xylooligosaccharide with low DP is prepared. The DP of finally obtained xylooligosaccharide is low by reasonably optimizing each process step, and xylobiose, xylotriose and xylotetraose are taken as main components, so that the functionality of xylooligosaccharide as a bifidus factor is enhanced. Tests verify that prepared xylooligosaccharide has a good proliferative effect on bifidobacterium animalis and lactobacillus casei. The process is simplified, the product quality is improved, and xylooligosaccharide has good application prospect.

The invention relates to probiotic preparations, and particularly relates to probiotic drops and a preparing method thereof. The probiotic drops include, by mass, 1-2.5% of unsaturated fatty acid, 85-90% of vegetable oil, 1-7% of phospholipid oil and 2-8% of a probiotic suspension. The probiotic suspension includes glycerin, Tween-80 and probiotic cells. The method includes S1) mixing and equilibrizing the probiotic cells with the glycerin and the Tween-80 according to a certain ratio, and allowing the mixture to stand for 15-30 min to achieve a system equilibrium state to obtain the probioticsuspension; and S2) fully uniformly emulsifying the probiotic suspension with the unsaturated fatty acid, the vegetable oil and the phospholipid oil according to a certain ratio to obtain the probiotic drops. The probiotic drops can improve microecology in oral cavities and noses of smoking persons and improve resistance of the smoking persons.

The invention provides a camellia chrysantha tissue culture propagation method. The camellia chrysantha tissue culture propagation method comprises the steps of obtaining aseptic seedlings, propagating the aseptic seedlings, culturing strong multiple shoots, expanding propagation and carrying out rooting culture, wherein camellia chrysantha embryos are selected as explants and then inoculated to a primary culture medium to obtain the aseptic seedlings of the camellia chrysantha; next, the aseptic seedlings are inoculated to a subculture multiplication culture medium and a strong shoot culture medium; the seedling propagation and strengthening processes are repeated and circulated until a large quantity of strong aseptic seedlings are obtained; finally, the aseptic seedlings are transplanted to a rooting culture medium for rooting culture. The camellia chrysantha tissue culture propagation method has the advantages that the vitrification problem of the aseptic seedlings in the camellia chrysantha tissue culture process is overcome, multiple subculture of materials can be realized, large-scale propagation is realized, the seedling growing period of the camellia chrysantha is shortened, the seedling growing materials are saved, the rooting rate is 95%-98%, and the transplanting survival rate is more than 90%; as a result, the camellia chrysantha tissue culture propagation method has excellent economic benefit, social benefit and ecologic benefit.

The invention discloses a fermented dairy product containing a compound prebiotic and probiotic group. Lactobacillus bulgaricus, Streptococcus thermophilus, Bifidobacterium lactis BB-12 and Lactobacillus acidophilus are added into the fermented dairy product. Two kinds of prebiotics, namely fructooligosaccharide and galactooligosacchride are also added into the fermented dairy product; and the content of the fructooligosaccharide is 0.25 to 60g and the content of the galactooligosacchride is 0.25 to 60g in 1,000g of fermented dairy product raw material, and the content sum of the fructooligosaccharide and the galactooligosacchride is 0.5 to 120g. By the fermented dairy product containing the compound prebiotic and probiotic group, the phenomenon that the excessive eating of a fermented dairy product may cause flatulence, exhaust and diarrhea of a small number of users when single prebiotics are used is avoided, and Bifidobacterium and lactobacillus in intestinal tracts have a good proliferation effect; and meanwhile, the fermented dairy product slightly proliferates enterococcus, enterobacter and Clostridium perfringens, and produces low pH values in the intestinal tracts.

The invention belongs to the technical field of plant tissue and cell culture, and in particular relates to a plant regeneration method by using leaves of Chinese rose as explants through a somatic cell embryogenesis path. The method comprises the following three culture stages: (1) culturing the Chinese rose explants on an induction medium, and directly inducing somatic embryos from the explants, wherein the explants are wrapped leaves of Chinese rose respectively; (2) inoculating the inoculate on a propagation culture medium for propagation and accelerating further differentiation thereof; and (3) inoculating the somatic embryos subjected to propagation culture to a somatic embryo seedling culture medium and a rooting culture medium and regenerating complete plants. The method has the advantages of wide sources of explants, convenient material selection and the like. The prepared somatic embryos are agrobacterium-mediated excellent receptors through genetic transformation and can be used for research on genetic transformation of the Chinese rose. The invention also relates to components of culture media for the method.

The invention relates to a chaffy dish base flavoring, and specifically relates to a nutritive chaffy dish base flavoring. The invention belongs to the field of foodstuff processing. According to the invention, spinach is added to the base flavoring. Spinach contains a large amount of beta carotene and iron, and is also an excellent source of vitamin B6, folic acid, iron and potassium. Spinach contains a large amount of anti-oxidants such as vitamin E and selenium element. Spinach has functions of aging resisting, cell proliferation promoting, brain function activating, vigor enhancing, brain aging preventing, and Alzheimer's disease preventing. Also, spinach has an antihypertensive effect, such that the base flavoring is suitable for the elderly to eat. According to the invention, xylitol is used for replacing edible sugar, such that the sugar content of the base flavoring is reduced. The base flavoring is suitable for various populations to eat, and has a nutritive function.

The invention discloses a molten salt reactor core, a molten salt reactor system, a fuel cycle system and a fuel cycle method. An active region of the molten salt reactor core has a respective independent transmutation region and a proliferation region, and the transmutation region is disposed in the proliferation region; the transmutation region is used for fast-spectrum transmutation of tran uranium nuclides under chlorine salt and no moderators conditions, and transferring high residual neutrons obtained by the transmutation to the proliferation region; the proliferation region is used forsuperheating-spectrum proliferation of nuclear fuel thorium under fluoride salt and graphite balls as the moderator conditions. The molten salt reactor core, the molten salt reactor system, the fuel circulation system and the fuel cycle method have an excellent thorium uranium proliferation capability and a transmutation capability, can efficiently transmute TRU in spent fuel, fully utilize the nuclear fuel thorium, and successfully solve current difficulties of shortage of nuclear fuel resources and accumulation of highly active wastes in nuclear development.

The present invention discloses a giant salamander active peptide and hyaluronic acid composition for effectively promoting fibroblast cell proliferation. A mass ratio of a giant salamander active peptide solution at a mass volume concentration of 1-10% and pH of 5-7 to hyaluronic acid is (1-8):1, molecular weight of the giant salamander active peptide is less than 2,000 Da, mole percentage of lysine and arginine are 7.2% and 9.7%, respectively, and molecular weight of the hyaluronic acid is 42.5-145 kDa. The giant salamander active peptide can be naturally cross-linked with the hyaluronic acid rich in multiple hydroxyl groups through hydrogen bonding, natural affinity of the hyaluronic acid and human skin cells is used, and the giant salamander active peptide and hyaluronic acid composition can effectively promote promotion of skin fibroblast proliferation and has broad application prospects in the fields of medicines and cosmetics,.

The invention provides mesenchymal stem cell injection liquid and a preparation method, which aim to solve the problem of how to use a mesenchymal stem cell to efficiently repair and improve damaged skin and fundamentally delay and stop skin cell aging. The injection liquid provided by the invention is prepared from the mesenchymal stem cell, DMSO, EGF, glutathione, vitamin C, human serum albumin,a ginkgo biloba extract and a menstruum. The mesenchymal stem cell is obtained through carrying out hungry culture on P2 to P5-generation mesenchymal stem cells and stimulating through an epidermal growth factor. The obtained mesenchymal stem cell has a good differentiative capacity and a good proliferation capacity, has a better differentiation potential, and is synergized with the DMSO, the EGF, the glutathione, the vitamin C, the human serum albumin and the ginkgo biloba extract, so that skin wrinkles and hyperpigmentation can be remarkably reduced.

The invention relates to CIK cell cryopreservation liquid. The CIK cell cryopreservation liquid is prepared from, by volume and concentration, 5-10% of DMSO, 1-2 mg / ml of grape seed extracts, 5-10 mg / ml of raffinose, 10-20% of human albumin, 40-70% of FBS and the balance PBS. Due to the fact that raffinose, grape seed extracts and human albumin are added, cell survival time is prolonged, and the cellular structure is prevented from being damaged by free radicals. By the adoption of the CIK cell cryopreservation liquid, cell viability can be better maintained, loss of cell surface antigens is reduced, and cells still have high multiplication capacity and killing activity after recovery.

The invention belongs to the technical field of preparation of probiotic products, and particularly relates to a preparation method of Bifidobacterium microcapsules, which comprises the following steps: 1. preparing a composite bacterial suspension; 2. dissolving a wall material; and 3. preparing the composite microcapsules. The invention provides a novel method for preparing Bifidobacterium powder microcapsules, removes the film-forming reaction of chitosan, and lowers the production cost. According to the invention, the Bifidobacterium is embedded into an enteric material isolate the outside air, so that the Bifidobacterium microcapsules are hardly dissolved in gastric acid and slowly release in the intestinal tract; and after the Bifidobacterium microcapsules are digested by the intestinal tract, the bioavailability is enhanced, and the survival rate is higher than the document report.

The invention relates to new use of B Salvianolic acid. The B Salvianolic acid has a strong inhibition effect to tyrosinase, thereby being used for whitening skin, removing freckle, resisting wrinkle, and treating hyperpigmentation diseases, and being applicable to food additives. The inhibition of the B Salvianolic acid to the activity of the tyrosinase is detected, the B Salvianolic acid is very high in the activity of the tyrosinase, and is better in stability, and the B Salvianolic acid can effectively inhibit the generation of the melanin, and can be taken as the natural skin-whitening agent for cosmetics. Meanwhile, the B Salvianolic acid can more preferably promote the skin cell proliferation function, and has the functions of beautifying and resisting wrinkle, thereby being applicable to the cosmetics with the functions of beautifying and resisting wrinkle.

The invention discloses a method for efficiently propagating dendrobium by using roots and a culture medium of the dendrobium. The method in the invention comprises the following steps of: selecting a root section of the dendrobium, and incubating and inducing the root section to obtain an embryonic callus, wherein the embryonic callus can be greatly multiplied through subculture; transferring the embryonic callus into a protocorm induction culture medium for induction and multiplication to obtain protocorm and bud seedlings; and transferring the protocorm and the bud seedlings into an adult seedling culture medium and culturing a dendrobium rooting plant. According to the method, MS is used as a basic culture medium; 6-BA and PIC are required to be added into the culture medium for the embryonic callus induction or subculture; CPPU and NAA are required to be added into the culture medium for the protocorm induction. The method for culturing regenerated plants by using the dendrobium roots provided by the invention is high in propagation coefficient, high in planting percentage and low in cost; a seedling growing process, a seedling robusting process and a rooting progress of the protocorm can be further finished; the plants are robust, and the roots are robust.

The invention provides a method for promoting growth of probiotics by adding rutin in a culture medium. According to the method, a probiotics strain (lactobacillus rhamnosus) is inoculated into a rutin-added improved MRS lactic acid bacteria culture medium for fermentation, wherein the improved MRS lactic acid bacteria culture medium includes the following components in percentage by weight: 2% of glucose, 2% of peptone, 1% of a beef extract, 0.5% of a yeast extract, 0.5% of sodium acetate, 0.2% of dipotassium phosphate, 0.2% of diammonium hydrogen citrate, 0.025% of magnesium sulfate, 0.005% of manganese sulfate, 0.3% of tween-80, and the pH is 7.0; 1g-5g of rutin (with the impurity of 99%) is added into each liter of the fermentation culture medium. According to the method, the fermentation speed of probiotics in fermentation liquid and the viable count of probiotics can be remarkably increased; after carrying out fermentation growth on probiotics for 24 hours, the maximal viable count of obtained probiotics fermentation liquid can reach about 10<9>CFU / mL; added rutin has the effects of decreasing capillary fragility, improving microcirculation, resisting radiation and free radicals and the like, and the functional effect of a cultured product can be substantially improved.

The invention relates to an injection organism repair material and relative production. Wherein, it comprises carrier support and seed cell adhered on the carrier support to form skeleton three-dimension structure and biological active composite; and it uses the injection chitose-beta-tricalcium phosphate composite as carrier support to couple seed cell; the seed cell is bone marrow substrate stem cell. The test has proved that the chitose-beta-tricalcium phosphate composite has better compatibility with BMSCs.