9results about How to "Proliferation effect is good" patented technology

The present application relates to the field of cell therapy and regenerative medicine, and in particular to a kind of old age adipose tissue-derived mesenchymal stem cells pretreated by MK8722, its preparation method and application.The method comprises the following steps: the old age adipose tissue-derived mesenchymal stem cells are inoculated in culture solution containing MK8722 to carry out in vitro treatment, and first cells are obtained;The culture solution containing MK8722 is removed, and the first cells are washed to obtain second cells;Second cells are cultured using culture solution without MK8722, and the old age adipose tissue-derived mesenchymal stem cells pretreated by MK8722 are obtained.The cells obtained by the method, relative to the old age adipose tissue-derived mesenchymal stem cells without the in vitro treatment, show at least one improvement in proliferation capacity, continuous passage amplification capacity, adipogenic differentiation capacity or chondrogenic differentiation capacity, and can be used to prepare products for treating or alleviating osteoarthritis.

This invention discloses a method for preparing Hu sheep bone polypeptides, belonging to the field of polypeptide preparation technology. Specifically, it involves pretreating fresh Hu sheep bones to obtain Hu sheep bone powder, and then enzymatically hydrolyzing the Hu sheep bone powder to obtain Hu sheep bone polypeptides. The enzymatic hydrolysis uses a complex protease, including trypsin and papain, with a mass ratio of trypsin to papain of 1:0.1-10. The enzymatic hydrolysis is purified using silica gel column separation. The silica gel column packing material includes silica gel or modified silica gel. In the preparation of modified silica gel, the silica gel is first activated with acid, then functionalized with 3-aminopropyltriethoxysilane, and then reacted with a modified monomer, cyclohexylvinyl ether, to obtain modified silica gel. This invention also discloses a method for preparing Hu sheep bone polypeptides with high hydroxyl radical scavenging rate, high calcium chelation rate, and good cell proliferation effect after calcium chelation.

This invention relates to the field of in vitro stem cell culture technology, specifically to a culture medium for the proliferation of muscle satellite cells, its culture method, and its application. The culture medium comprises a basal medium, fetal bovine serum, and plant-derived active substances; the plant-derived active substances include at least one selected from apigenin, resveratrol, and quercetin; the volume percentage of fetal bovine serum in the culture medium is 2%-5%. This invention, by adding plant-derived active substances, effectively maintains cell stemness and significantly promotes cell proliferation at low serum concentrations.

The invention belongs to the technical field of plant tissue culture, and discloses a Paeonia fulva tissue culture and rapid propagation method for inducing cluster buds by using immature embryos, which comprises the following steps: collecting fresh seeds at a specific development stage, sterilizing, and stripping embryos aseptically; inoculating the embryos into a germination culture medium containing 6-BA, IAA and GA3 to start culture; after radicle tail ends of the germinating embryos are cut off, the embryos are directionally inoculated to a subculture medium according to the original polarity, and sterile seedlings are obtained; transferring the seedlings into an induction culture medium containing 6-BA, IAA and charcoal to induce cluster buds, and further proliferating; cutting robust tender shoots, removing cotyledons, and inoculating into a rooting culture medium containing IBA (indolebutyric acid), NAA (naphthylacetic acid) and charcoal to induce rooting; and finally, carrying out strong seedling culture and domestication transplanting to obtain a survived plant. According to the method, the deep dormancy obstacle of the seeds is broken through, efficient and stable regeneration from embryos to seedlings is achieved, the breeding cycle is short, the rooting and transplanting survival rate is high, and the method is suitable for large-scale protection and production of rare resources.

This invention discloses a method for rapid propagation of *Prunus campanulata* via tissue culture and regeneration of adventitious buds. This invention pioneers a system for rapid propagation and organ regeneration of *Prunus campanulata* via tissue culture, utilizing its vegetative organs, stem segments, leaves, or seeds as explants. This results in a primary induction rate as high as 74.4%, a proliferation coefficient of approximately 3.9, and a rooting rate of 83.5%, achieving highly efficient and rapid propagation. Unrooted, robust buds can also be used as scions for grafting propagation. In the seed disinfection process, the plant tissue culture antibacterial agent PPM is applied for the first time to disinfect seeds of the *Prunus* subgenus, achieving good control of the contamination rate and significantly reducing it. The invention also explores the number of times mercuric chloride disinfectant can be reused for the first time, finding that its disinfection effect decreases with increasing reuse frequency, with a significant decrease in disinfection effect after three or more uses. Furthermore, the same culture medium is used for obtaining sterile explants, inducing sterile seedlings, and propagation culture, simplifying the operation process and reducing production costs.

The invention discloses miR-184 and application of the miR-184 in regulating and controlling the reproduction of female diaphorina citri. The nucleotide sequence of the micromolecule miR-184 provided by the invention is shown as SEQ ID NO.4, and the micromolecule miR-184 is highly expressed in the ovary of the female diaphorina citri. By feeding the miR-184 agonist, the egg laying amount of the diaphorina citri is obviously reduced, the development of the ovary is inhibited, the titer of the Candidatus Liberibacter asiaticum in the ovary is obviously reduced, and the miR-184 agonist has a good application prospect in the aspects of regulating and controlling the reproduction of female diaphorina citri, preventing and treating the female diaphorina citri, preventing the Candidatus Liberibacter asiaticum and the like.

This invention relates to the field of plant tissue culture technology, specifically to a culture medium and method for inducing and regenerating tiger nut callus. This invention creatively combines growth regulators (2,4-D, 6-BA, NAA, and ZT) in the culture medium for three different stages of tiger nut callus induction, regeneration, and differentiation, and optimizes the selection of the best explants. This allows each component to fully synergistically enhance its effect at each stage, resulting in high callus induction rate, good growth status, low browning degree, good proliferation effect, and high differentiation rate and differentiation coefficient. Furthermore, no growth regulators are required during rooting induction, resulting in lower costs. This invention lays the foundation for tiger nut gene function research, variety genetic improvement, and germplasm resource optimization.

The present application relates to the technical field of industrial microorganisms, and particularly relates to a lacticaseibacillus paracasei ZF616 and application thereof, and further relates to application of the lacticaseibacillus paracasei in soy sauce production.The lacticaseibacillus paracasei ZF616 is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No: 62634, and the preservation address is No. 59, Building, Guangzhou, Guangdong Province.The strain of the present application has the advantages of being capable of increasing the content of malic acid in fermented soy sauce, and not affecting other components in fermented soy sauce after being added, and when the strain is applied to a soy sauce fermentation process, the obtained soy sauce not only retains the rich soy sauce flavor of fermented soy sauce, but also increases the pleasant sour taste of the soy sauce, the taste is more sour, the sweet and sour taste is more full and coordinated, and the thickness of the soy sauce is improved.

This invention discloses a method for preparing a *Lactobacillus plantarum* enrichment agent and its application, relating to the field of microbial technology. The preparation method includes: extracting traditional Chinese medicine to obtain medicinal residue; enzymatically hydrolyzing the residue; adjusting the pH to neutral to obtain the enrichment agent; wherein the traditional Chinese medicine is selected from one or more of the following: dried tangerine peel, white hyacinth bean, coix seed, polygonatum, lotus leaf, dendrobium officinale, licorice, atractylodes macrocephala, poria cocos, chicken gizzard lining, roasted malt, wolfberry, kudzu root, and clove. The *Lactobacillus plantarum* enrichment agent provided by this invention uses medicinal residue as raw material and is specifically processed using a compound enzyme. It has low raw material costs, high product added value, and can significantly increase the viable count of *Lactobacillus plantarum* in a unit volume of fermentation broth, effectively reducing the cost of preparing highly active *Lactobacillus plantarum* inoculants.