46results about How to "Proliferation effect is good" patented technology

The invention relates to a composite metabiotic inoculant for promoting vaginal micro-ecological balance and application thereof. Strain in the composite metabiotic inoculant for promoting vaginal micro-ecological balance comprises an inactivated LA88 strain and an inactivated N13 strain. The invention develops a brand-new probiotic intervention strategy for promoting the micro-ecological balance of the vagina, namely, the inactivated lactobacillus acidophilus LA88 strain and the inactivated lactobacillus plantarum N13 strain are compounded to form a brand-new composite metabiotic bacterial agent, and the composite metabiotic bacterial agent has a relatively good proliferation effect on various beneficial bacteria of the vagina, and can be used for preparing the probiotics for promoting the micro-ecological balance of the vagina. The traditional Chinese medicine composition has a strong inhibition effect on various vaginal pathogenic bacteria, and can promote recovery of a healthy microbial community structure.

The invention relates to the technical field of freeze-dried preparations, and provides exosome freeze-dried powder as well as a preparation method and application thereof. The exosome freeze-drying preparation is prepared by mixing an exosome solution and a freeze-drying protection solution and then freeze-drying the mixture, the concentration of the exosome solution in the mixed solution is 0.1-10% V / V, and the freeze-drying protection solution comprises 0.4-4% W / V of saccharides and 0.25-4% W / V of alcohols; the saccharide is trehalose, cane sugar or lactose; the alcohol is mannitol, sorbitol or a combination of mannitol and polyethylene glycol; and the solvent is sterilized water for injection or a PBS buffer solution. The exosome freeze-dried powder is stable in activity, small in irritation, good in safety and good in fibroblast proliferation promoting capacity, when the exosome freeze-dried powder is applied to supporting essence or masks in the medical beauty industry, the exosome activity of the exosome freeze-dried powder is still kept stable, and the application prospect is good.

The invention belongs to the technical field of bioengineering, and particularly relates to recombinant III-type humanized collagen as well as a coding gene and application thereof. According to the invention, a novel III-type humanized collagen sequence is designed by adopting an advanced AI technology and molecular dynamics simulation, so that the recombinant III-type humanized collagen product is ensured to have high stability and degradation resistance. According to the invention, the screened new III-type humanized collagen sequence is transferred to the pichia pastoris strain for recombinant expression, and the prepared recombinant III-type humanized collagen has the advantages of strong stability, high expression quantity and good hydrophilicity, and meanwhile, the recombinant III-type humanized collagen also has obvious effects of promoting cell proliferation and cell adhesion.

The invention belongs to the technical field of bioengineering, and particularly relates to recombinant III-type humanized collagen and application thereof. Area fragments with good hydrophilicity in a human III-type collagen alpha chain are selected and sequentially connected to serve as expression protein, the amino acid sequence is 100% identical to that of a corresponding part of natural human III-type collagen, and immunological rejection cannot be generated when the human III-type collagen is applied to a human body. According to the invention, the screened new III-type humanized collagen sequence is transferred into pichia pastoris for recombinant expression, the prepared recombinant III-type humanized collagen has the advantages of strong stability, high expression quantity and good hydrophilicity, and meanwhile, the recombinant III-type humanized collagen also has obvious effects of promoting cell proliferation and cell adhesion.

The invention provides a novel TPMS porous structure interbody fusion cage, and relates to the technical field of medical materials. The interbody fusion cage is designed by using a porous frame structure with different unit cell sizes and porosities, so that the biocompatibility of the interbody fusion cage is enhanced, the interbody fusion cage has higher mechanical properties, large static compression load and low elastic modulus, the elastic modulus is closer to that of human cortical bones, the stability of an implant in the early stage of osseointegration is improved, and the interbody fusion cage is suitable for being used in the field of osteosynthesis. Under the same porosity, the elastic modulus is lower, and the comprehensive performance is excellent.

The invention relates to the field of biological medicine, in particular to a water-soluble alginate microcarrier as well as a preparation method and application thereof. The alginate microcarrier comprises a carrier core structure and a shell surface modifier, the micro-carrier core layer is formed by carrying out plasma crosslinking on water-soluble alginate, ethylenediamine tetraacetic acid salt, nitrilotriacetic acid salt, diethylene triamine pentaacetic acid and water-soluble divalent metal salt, and light-sensitive alginate and a photoinitiator can also be added, so that the micro-carrier core layer has two crosslinking networks; the shell layer is composed of one of gelatin, collagen, fibrinogen, albumin and fibrin, or the gelatin, the collagen, the fibrinogen, the albumin and the fibrin and other biocompatible polymers; the obtained microcarrier has the characteristics of high transparency and rapid degradation, and adherent cells can be amplified in vitro; the microcarrier obtained in the invention can be used for mesenchymal stem cell culture, and can be rapidly degraded under a mild cell condition to harvest cells.

The invention provides a recombinant human I-type collagen fragment COLI-23H, and the amino acid sequence of the recombinant human I-type collagen fragment COLI-23H is as shown in SEQ ID NO.3. The invention also provides a coding gene of the protein COLI-23H, a pichia pastoris engineering bacterium comprising the gene and an engineering bacterium fermentation process. According to the invention, a sequence fragment is screened from an original human-derived I-type collagen sequence, and the nucleotide sequence of the fragment is optimized, so that the fragment is more suitable for a pichia pastoris expression system, a recombinant I-type collagen high-yield strain is obtained, and a fermentation process of the recombinant I-type collagen high-yield strain is optimized. The recombinant human I-type collagen fragment COLI-23H disclosed by the invention has excellent biological activity, can be subjected to recombinant expression and is high in yield. Meanwhile, the invention also provides application of the recombinant human I-type collagen COLI-23H in the fields of wound repair, medical aesthetic plastic surgery and cosmetics.

The invention discloses an apple rootstock tissue culture seedling raising method, and relates to the technical field of tissue culture. The method comprises the following steps: (1) washing annual robust branches with tender shoots in a dormancy period with running water, soaking with soapy water, cleaning, obliquely shearing into stem segments, soaking with alcohol, washing, sterilizing, washing, absorbing moisture, and shearing off wounds to obtain pretreated explants; (2) inoculating the pretreated explant into a differential culture medium, and culturing to obtain an adventitious bud; (3) transferring the adventitious buds into a proliferation culture medium, and performing dark culture and light culture to obtain adventitious bud clusters; (4) inoculating the adventitious bud clusters into a rooting culture medium, and culturing to obtain tissue culture seedlings; and (5) hardening the tissue culture seedlings, washing off the culture medium, disinfecting, putting into a culture medium, culturing to obtain apple seedlings, and transplanting to outdoor soil. According to the method, the seedlings capable of being transplanted outdoors can be rapidly obtained, and the problems of infectious microbe pollution, low survival rate, poor rooting effect and the like in existing tissue culture seedling culture are effectively solved.

The invention discloses a preparation method and application of a milk-derived immunomodulatory peptide, milk-derived casein is taken as a raw material, known immunomodulatory peptide is screened, virtual enzymolysis is performed on the known immunomodulatory peptide, and the immunomodulatory peptide is separated and prepared by a verification and freeze drying method. The casein can be subjected to targeted enzymolysis, the relative content and variety of the immunomodulatory peptide are increased, the situation that the immunomodulatory effect of other immunomodulatory casein peptide products is measured after pure enzymolysis, and the efficacy of the immunomodulatory peptide is not enhanced is reduced, the better immunomodulatory effect is achieved, and the immune cell proliferation effect is effectively promoted.

The invention discloses an apple rootstock tissue culture seedling raising method, and relates to the technical field of tissue culture. The method comprises the following steps: (1) washing annual robust branches with tender shoots in a dormancy period with running water, soaking with soapy water, cleaning, obliquely shearing into stem segments, soaking with alcohol, washing, sterilizing, washing, absorbing moisture, and shearing off wounds to obtain pretreated explants; (2) inoculating the pretreated explant into a differential culture medium, and culturing to obtain an adventitious bud; (3) transferring the adventitious buds into a proliferation culture medium, and performing dark culture and light culture to obtain adventitious bud clusters; (4) inoculating the adventitious bud clusters into a rooting culture medium, and culturing to obtain tissue culture seedlings; and (5) hardening the tissue culture seedlings, washing off the culture medium, disinfecting, putting into a culture medium, culturing to obtain apple seedlings, and transplanting to outdoor soil. According to the method, the seedlings capable of being transplanted outdoors can be rapidly obtained, and the problems of infectious microbe pollution, low survival rate, poor rooting effect and the like in existing tissue culture seedling culture are effectively solved.

The invention discloses application of EPO (erythropoietin) in preparation of a medicine for preventing and treating corneal injury, belongs to the technical field of medicines, and focuses on innovative application of EPO in preparation of the medicine for preventing and treating corneal injury. The cornea is used as a key structure of eyes and is easily damaged by various factors such as mechanical trauma, chemical injury, infection and diabetes, and more than ten millions of people in the world are troubled by cornea blindness. The invention finds that EPO has a remarkable treatment effect on corneal injury, and can promote proliferation and migration of corneal epithelium and stromal cells, increase the generation of extracellular matrix and enhance the tolerance of corneal epithelium cells and corneal stromal cells to high-level sugar. The medicine can be a self-freeze-drying preparation or a solution preparation, the administration modes are diversified, and various eye local injection, eye drop, gene therapy and the like are covered. Refined treatment schemes are provided for corneal injuries of different pathogenesis, a new way is developed for treatment of the corneal injuries, the corneal blindness rate is expected to be reduced, and the development of ophthalmic medicine is promoted.

The present invention belongs to the field of biotechnology, and specifically relates to a kind of adipose mesenchymal stem cell cryopreservation solution and its application. The above-mentioned adipose mesenchymal stem cell cryopreservation solution is composed of a culture medium and a cryoprotectant, and the composition of the cryoprotectant is: Exendin-4, sofalcone, rosmarinic acid, glycerol, and the weight ratio of each component of Exendin-4, sofalcone, rosmarinic acid, and glycerol in the cryoprotectant is 1:0.8~1.7:0.4~1.2:50~70. The present invention has been found through experiments that Exendin-4, sofalcone, and rosmarinic acid can make adipose mesenchymal stem cells have good viability, adhesion rate and proliferation ability after cryopreservation and recovery.

The present application relates to the field of cell therapy and regenerative medicine, and in particular to a kind of old age adipose tissue-derived mesenchymal stem cells pretreated by MK8722, its preparation method and application.The method comprises the following steps: the old age adipose tissue-derived mesenchymal stem cells are inoculated in culture solution containing MK8722 to carry out in vitro treatment, and first cells are obtained;The culture solution containing MK8722 is removed, and the first cells are washed to obtain second cells;Second cells are cultured using culture solution without MK8722, and the old age adipose tissue-derived mesenchymal stem cells pretreated by MK8722 are obtained.The cells obtained by the method, relative to the old age adipose tissue-derived mesenchymal stem cells without the in vitro treatment, show at least one improvement in proliferation capacity, continuous passage amplification capacity, adipogenic differentiation capacity or chondrogenic differentiation capacity, and can be used to prepare products for treating or alleviating osteoarthritis.

The invention relates to the technical field of biological material preparation, and particularly discloses hyaluronic acid hydrogel as well as a preparation method and application thereof in preparation of a corneal injury repair material. The preparation method of the hyaluronic acid hydrogel comprises the following steps: S1, preparing a hyaluronic acid solution; s2, adding a cross-linking agent into the hyaluronic acid solution, and adjusting to acidic pH to obtain a solution A; s3, adding a carboxyl activator into the solution A, and stirring to obtain hyaluronic acid hydrogel; a preparation method of the hyaluronic acid solution in the step S1 comprises the following steps: taking hyaluronic acid, adding the hyaluronic acid into the traditional Chinese medicine extracting solution, and uniformly stirring to obtain the hyaluronic acid solution. Researches show that the hyaluronic acid hydrogel prepared by the method disclosed by the invention has the effect of promoting proliferation of corneal epithelial cells; therefore, the material has important application value when being used as a corneal injury repair material.

The invention relates to hair growth lotion containing natural fungus extract and a preparation method thereof, and belongs to the technical field of alopecia treatment. The hair-growing water provided by the invention contains a core effective component, namely the sarcodon aspratus polysaccharide, and a liquid auxiliary component. The sarcodon aspratus polysaccharide is extracted from sarcodon aspratus, the molecular weight range of the sarcodon aspratus polysaccharide is 10,000-30,000 Daltons, and the sarcodon aspratus polysaccharide has the biological activity of promoting proliferation and migration of hair follicle cells. The auxiliary components comprise 5% of cocamidopropyl betaine, 2% of glycerin, 0.5% of tea tree essential oil and 92.5% of distilled water, and the components act together to improve scalp health and promote hair growth. The hair growth lotion provided by the invention can provide a new and effective choice for clinical treatment of alopecia, and is especially suitable for users who pursue natural therapy. In addition, the method disclosed by the invention is simple and convenient, high in cost benefit and wide in market application prospect.

The invention provides a preparation method of fructo-oligosaccharide, and belongs to the technical field of fructo-oligosaccharide synthesis. The method comprises the following steps: (1) preparing a reaction stock solution; (2) adding fructosyl transferase into the reaction stock solution, and then carrying out constant-temperature water bath reaction; supplementing the sugar solution in the reaction process to continue the reaction to obtain a first-process saccharified solution; (3) filtering the first-process saccharification liquid by adopting a semi-permeable membrane to obtain trapped fluid and filtrate; (4) performing a constant-temperature water bath reaction on the filtrate to obtain a second-process saccharification liquid, and then mixing the second-process saccharification liquid with the trapped fluid to obtain a fructo-oligosaccharide crude liquid; (5) carrying out decoloration, desalination, chromatographic separation, evaporation concentration and drying on the fructo-oligosaccharide crude liquid to prepare fructo-oligosaccharide powder; the reaction time is shortened, the utilization rate of fructose is increased, generation of kestose in a system is promoted, the content of kestose in fructo-oligosaccharide is increased, and the water activity of fructo-oligosaccharide is reduced.

This invention discloses a method for preparing Hu sheep bone polypeptides, belonging to the field of polypeptide preparation technology. Specifically, it involves pretreating fresh Hu sheep bones to obtain Hu sheep bone powder, and then enzymatically hydrolyzing the Hu sheep bone powder to obtain Hu sheep bone polypeptides. The enzymatic hydrolysis uses a complex protease, including trypsin and papain, with a mass ratio of trypsin to papain of 1:0.1-10. The enzymatic hydrolysis is purified using silica gel column separation. The silica gel column packing material includes silica gel or modified silica gel. In the preparation of modified silica gel, the silica gel is first activated with acid, then functionalized with 3-aminopropyltriethoxysilane, and then reacted with a modified monomer, cyclohexylvinyl ether, to obtain modified silica gel. This invention also discloses a method for preparing Hu sheep bone polypeptides with high hydroxyl radical scavenging rate, high calcium chelation rate, and good cell proliferation effect after calcium chelation.

The present invention discloses the use of ethyl 4-hydroxy-2-quinolinecarboxylate in the preparation of a drug or reagent for promoting hepatocyte proliferation. The present invention confirms through in vitro cell experiments that ethyl 4-hydroxy-2-quinolinecarboxylate has a significant effect on promoting hepatocyte proliferation and is non-toxic to cells in a concentration range of 1 to 500 μM. Ethyl 4-hydroxy-2-quinolinecarboxylate can be used to promote hepatocyte proliferation in humans, rats or other animals, including the process of hepatocyte proliferation and the process of hepatocyte regeneration after partial liver resection.

This invention relates to the field of in vitro stem cell culture technology, specifically to a culture medium for the proliferation of muscle satellite cells, its culture method, and its application. The culture medium comprises a basal medium, fetal bovine serum, and plant-derived active substances; the plant-derived active substances include at least one selected from apigenin, resveratrol, and quercetin; the volume percentage of fetal bovine serum in the culture medium is 2%-5%. This invention, by adding plant-derived active substances, effectively maintains cell stemness and significantly promotes cell proliferation at low serum concentrations.

The invention relates to the field of wound tissue repair, and discloses an ANGPTL-7 recombinant protein, a gene, a recombinant vector, a recombinant strain, a composition and applications thereof. The recombinant protein has an amino acid sequence selected from any one of the following (a)-(c): (a) an amino acid sequence as shown in SEQ ID NO: 1; (b) a derivative protein obtained by substitution, deletion or addition of at least one amino acid to the amino acid sequence represented by SEQ ID NO: 1, wherein the protein activity of the derivative protein is not lower than 95% of the protein activity of (a); (c) an amino acid sequence of which the amino terminal and / or carboxyl terminal of the amino acid sequence (a) or (b) are / is connected with a label; and (d) an amino acid sequence formed by connecting a signal sequence to the amino terminal of the amino acid sequence (a) or (b). The recombinant protein can promote tissue repair and accelerate wound healing, and is expected to be widely applied to clinical treatment.

The invention discloses establishment and application of a human ovarian ectopic inner membrane cell strain (HOEEC) based on SV40T gene transfection, and relates to the technical field of biology, and the establishment comprises the following steps: S1, collecting a specimen; s2, isolated culture of human ovarian ectopic inner membrane primary cells; s3, amplification and extraction of plasmids; s4, performing lentivirus transfection to establish a human ovarian ectopic inner membrane cell strain; the method comprises the following steps: firstly, extracting and separating to obtain primary cells of the human ectopic ovarian endometrium, then establishing the human ectopic ovarian endometrium cell strain by transfecting SV40T, comparing the differences between the HOEEC and the primary cells in the aspects of cellular morphology, passage condition, multiplication capacity, invasion capacity, hormone receptor expression and estrogen reaction, and detecting the number and morphology of HOEEC chromosomes and the existence of in-vivo tumorigenicity. A new human ovarian ectopic endometrial cell strain is established by transfecting the SV40T gene for the first time, and an in-vitro model is provided for studying the pathogenesis of ovarian endometriosis.

The present invention belongs to the field of cell culture technology, and specifically relates to a combination of bone marrow mesenchymal stem cell culture media and a culture method. The bone marrow mesenchymal stem cell culture media combination of the present invention includes culture media I and culture media II; the culture media I is a DMEM / F12 culture media with added asterone, melatonin, linoleic acid, L-glutamine, bFGF, insulin, and astragalus polysaccharide; the culture media II is a DMEM / F12 culture media with added ACE2, linoleic acid, L-glutamine, bFGF, insulin, and astragalus polysaccharide. The present invention provides a method for culturing bone marrow mesenchymal stem cells, improves the culture media used for bone marrow mesenchymal stem cells, and specifically designs primary culture media and passage culture media for different cell culture processes, and the two cooperate with each other, and the bone marrow mesenchymal stem cells obtained by culture have a good growth state and better proliferation ability and osteogenic differentiation potential.

The invention discloses a culture medium of umbilical cord mesenchymal stem cells and an isolated culture method of the umbilical cord mesenchymal stem cells, and belongs to the technical field of biological medicines.The culture medium is high in proliferation speed, large in quantity and stable in phenotype due to mutual cooperation of all components, and on the premise that animal origin, antibiotics and serum are not added in the whole preparation process, the yield of the umbilical cord mesenchymal stem cells is increased. The umbilical cord mesenchymal stem cells with high purity, high motility rate and high yield are separated from Wharton's jelly. By improving the purity and motility rate of the umbilical cord mesenchymal stem cells, the effectiveness and timeliness of clinical treatment of diseases by the umbilical cord mesenchymal stem cells are improved. Meanwhile, the cost of clinical use of stem cell treatment is reduced by increasing the yield of the umbilical cord mesenchymal stem cells. The umbilical cord mesenchymal stem cells obtained through separation culture have good multiplication capacity and multidirectional differentiation potential, and the umbilical cord mesenchymal stem cells obtained through separation culture can be rapidly induced and differentiated into osteoblasts, adipoblasts and chondroblasts in an induced differentiation culture medium.

The present invention provides an adenine Cu-MOF-based hydrogel composite material and a preparation method and application thereof, and relates to the technical field of MOF materials. The adenine Cu-MOF-based hydrogel composite material is composed of a copper metal organic framework loaded on a hydrogel; wherein the copper metal organic framework is [Cu2(Ad)2(SA)]·3DMA; wherein the hydrogel is methacrylated gelatin; wherein the mass ratio of the copper metal organic framework to the hydrogel is (0.1-1):(5-15). The adenine Cu-MOF-based hydrogel composite material of the present invention has good biocompatibility and stability, and the MOF has an obvious effect of promoting keratinocyte proliferation at an appropriate concentration, and has the potential to promote wound healing.

The invention belongs to the technical field of plant tissue culture, and discloses a Paeonia fulva tissue culture and rapid propagation method for inducing cluster buds by using immature embryos, which comprises the following steps: collecting fresh seeds at a specific development stage, sterilizing, and stripping embryos aseptically; inoculating the embryos into a germination culture medium containing 6-BA, IAA and GA3 to start culture; after radicle tail ends of the germinating embryos are cut off, the embryos are directionally inoculated to a subculture medium according to the original polarity, and sterile seedlings are obtained; transferring the seedlings into an induction culture medium containing 6-BA, IAA and charcoal to induce cluster buds, and further proliferating; cutting robust tender shoots, removing cotyledons, and inoculating into a rooting culture medium containing IBA (indolebutyric acid), NAA (naphthylacetic acid) and charcoal to induce rooting; and finally, carrying out strong seedling culture and domestication transplanting to obtain a survived plant. According to the method, the deep dormancy obstacle of the seeds is broken through, efficient and stable regeneration from embryos to seedlings is achieved, the breeding cycle is short, the rooting and transplanting survival rate is high, and the method is suitable for large-scale protection and production of rare resources.

The invention discloses a kind of efficient propagation method of Codonopsis pilosula, by using culture medium A in bottle to complete callus induction, then using culture medium B to complete the induction and proliferation steps of adventitious buds, the proliferation coefficient reaches 45.0-50.0;Then cooperate with "micro-cutting" outside the bottle, so that the rooting rate of adventitious buds reaches more than 90%, and subsequent domestication and transplanting steps can be smoothly carried out, and then after continuing to grow and grow, the rooting seedlings with thick roots and strong seedlings are obtained, and the seedling hardening and transplanting are carried out, and the survival rate after transplanting is also more than 90%. The present invention not only simplifies the tissue culture production process of Codonopsis pilosula seedlings, and the proliferation coefficient is much higher than the existing reports of other species of the same family, and the efficient "micro-cutting" rooting system combines adventitious root induction and domestication, greatly shortens the seedling raising cycle of Codonopsis pilosula tissue culture seedlings, solves the low proliferation coefficient in the tissue culture process of Codonopsis pilosula plants in Campanulaceae, the phenomenon such as difficult rooting and domestication, and low cost, short cycle, high quality and survival rate.

The invention provides a porcine rotavirus G5 type and application thereof. According to the present invention, the porcine rotavirus G5 type SC05 strain is separated from the porcine intestinal tract tissue, and is subjected to passage purification to obtain the porcine rotavirus G5 type SC05 strain, and the microbial preservation number of the porcine rotavirus G5 type SC05 strain is CGMCC No.46179; the isolated strain can stably proliferate on passage cells to generate typical cytopathy. The G5 type isolated strain of the porcine rotavirus disclosed by the invention has excellent immunogenicity; the vaccine prepared from the isolated strain can induce piglets to generate high-level neutralizing antibodies; meanwhile, the ELISA kit prepared from the isolated strain can be used for well detecting the porcine rotavirus G5 type antibody in serum.

An organoid production method includes culturing a human stem cell in a culture medium containing a cyclic peptide having an amino acid sequence set forth in Formula (1) or a pharmaceutically acceptable salt of the cyclic peptide [in Formula (1), X1 to X6 each represent a specific modified amino acid, X7 represents any amino acid residue, R is absent or represents a C-terminal modification group, n is an integer of 0 or 1, PeG is N-(2-phenylethyl)-glycine, and Nal1 is β-(1-naphthyl)-L-alanine].

The invention relates to the technical field of plant seedling culture, and discloses a method for artificially and efficiently breeding anoectochilus roxburghii by using protocorm-like bodies, which specifically comprises the following steps: step (1), obtaining explants; step (2), disinfecting the explants; step (3): pre-culturing the explant: thickening the explant material in a culture medium A, and expanding joints; step (4), inducing the explants in a culture medium B to generate a large number of protocorm-like bodies, further proliferating the protocorm-like bodies to generate new protocorm-like bodies, developing into seedlings, and enabling the proliferation coefficient to reach 35.0 or above; (5) rooting culture: cutting clumpy buds developed from protocorm-like bodies in the step (4) into proper sizes, and inoculating the clumpy buds into a culture medium C to induce rooting; and (6) domesticating and transplanting. According to the induction method, the protocorm-like body can be directly induced from the explant, seed germination is not needed, the cost is low, the period is short, the seedling quality and the survival rate are high, and application and popularization value is achieved.

This invention discloses a method for rapid propagation of *Prunus campanulata* via tissue culture and regeneration of adventitious buds. This invention pioneers a system for rapid propagation and organ regeneration of *Prunus campanulata* via tissue culture, utilizing its vegetative organs, stem segments, leaves, or seeds as explants. This results in a primary induction rate as high as 74.4%, a proliferation coefficient of approximately 3.9, and a rooting rate of 83.5%, achieving highly efficient and rapid propagation. Unrooted, robust buds can also be used as scions for grafting propagation. In the seed disinfection process, the plant tissue culture antibacterial agent PPM is applied for the first time to disinfect seeds of the *Prunus* subgenus, achieving good control of the contamination rate and significantly reducing it. The invention also explores the number of times mercuric chloride disinfectant can be reused for the first time, finding that its disinfection effect decreases with increasing reuse frequency, with a significant decrease in disinfection effect after three or more uses. Furthermore, the same culture medium is used for obtaining sterile explants, inducing sterile seedlings, and propagation culture, simplifying the operation process and reducing production costs.