72 results about "Organ regeneration" patented technology

The present invention provides novel compositions and methods for pharmaceutical or nutraceutical use in an animal, preferably in a human. In one aspect, compositions and methods are provided for promoting cell growth, tissue repair and organ regeneration, preferably in vivo and in situ. The composition comprises a sterol compound dissolved in oil at a concentration at least 0.5% by weight. The compositions may be used in the treatment of various conditions caused by injury, diseases and aging. As shown clinically, the methodology disclosed in the present invention was used successfully to regenerate or clone a new organ through cultivation of regenerative stem cells in vivo and in situ.

RNA prepared by in vitro transcription using a polymerase chain reaction (PCR)-generated template can be introduced into a cell to modulate cell activity. This method is useful in de-differentiating somatic cells to pluripotent, multipotent, or unipotent cells; re-differentiating stem cells into differentiated cells; or reprogramming of somatic cells to modulate cell activities such as metabolism. Cells can also be transfected with inhibitory RNAs, such as small interfering RNA (siRNA) or micro RNA (miRNA), or combinations thereof to induce reprogramming of somatic cells. For example, target cells are isolated from a donor, contacted with one or more RNA's causing the cells to be de-differentiated, re-differentiated, or reprogrammed in vitro, and administered to a patient in need thereof. The resulting cells are useful for treating one or more symptoms of a variety of diseases and disorders, for organ regeneration, and for restoration of the immune system.

The invention features methods for increasing or maintaining the number of functional cells of a predetermined type in a mammal (e.g., a human patient), for example, the insulin producing cells of the pancreas, liver cells, spleen cells, or bone cells, that has injured or damaged cells of the predetermined type or is deficient in cells of the predetermined type.

The present invention relates to methods, reagents, and kits for assessing organ damage, such as damage due to ischemia reperfusion injury, in the course of a transplantation therapy and/or for assessing organ regeneration following transplantation therapy. The invention provides a method for determining an index of organ health in the course of transplantation therapy comprising measuring the expression level of peripheral-type benzodiazepine receptor (PBR) in the organ. Measuring the expression level of PBR is also useful for assessing the progress of organ regeneration in the course of transplantation therapy by comparing the index of organ health. The expression level of PBR may be used as a predictor of the outcome of transplantation therapy.

The invention belongs to the biology and biological technical field, and discloses a plant in-situ regeneration and agrobacterium transformation method. Agrobacterium is coated on a wound of a plant root stock, a callus which is generated by inducing a plant in-vitro tissue on a culture medium is used as a scion, and the scion is grafted onto the root stock through a cleft graft method. Root stock wound cells can realize in-situ organ regeneration under the induction of an exogenous callus, so that a transgenic regeneration bud can be obtained. Compared with the traditional plant genetic transformation method combining the tissue culture, a one-by-one genotype fumbling tissue culture condition can be avoided, a novel technological platform can be provided to the genetic transformation of plants, meanwhile, a novel plant regeneration system is established, and a research system is provided to the study of plant development biology.

A self-contained circulation system, which supports the formation of capillaries in capillary growth sections and allows the formation of micro organoids and/or micro tissue sections for monitoring the effect of one or more test compounds and determining efficacy, side-effects, biosafety, metabolites, mode of action or organ regeneration as well as methods of establishing such micro organoids and/or micro tissue in the self-contained circulation system.

In one embodiment, the invention provides a method of regenerating organs and tissues in a subject suffering from one or more organ or tissue manifestations of glucose supply side associated metabolic syndrome, the method comprising: (a) confirming that the subject suffers from or is at risk for suffering from organ and/or tissue damage associated with a glucose supply side associated metabolic syndrome; and (b) co-administering to the subject an effective amount of a pharmaceutical composition comprising a first and optionally a second active composition, said first active composition comprising an ileal brake hormone releasing substance encapsulated within an enteric coating which releases said substance within said subject's ileum and ascending colon causing release of at least one ileal brake hormone from L-cells of said subject, said optional second active composition being formulated in immediate and/or early release form in an over coating onto said enteric coating, wherein said second composition is beneficial to at least one aspect of said subject's metabolic syndrome manifestations. Coadministration methods with a second pharmaceutical composition are also disclosed.

A composition for in-situ culture and regeneration of stem cells in vivo to repair tissue and regenerate organ, especially suitable for those in vitro, is prepared from the oil-soluble sterol compounds (at least 0.01 wt.%).

The invention provides a method for providing a vascular system in biological tissue in vitro. The method comprises cocultivation of the biological tissue with vascular cells and mesenchymal cells. Biological tissue in which a vascular system has been provided by said method. A method for producing a tissue or organ, the method comprising transplantation of the biological tissue in a non-human animal and inducing differentiation to a tissue or organ in which a vascular network has been constructed. A tissue or organ regeneration or function-restoring method comprising transplantation of the biological tissue in a human or non-human animal and inducing differentiation to a tissue or organ in which a vascular network has been constructed. A method for producing a non-human chimera animal, the method comprising transplantation of the biological tissue to a non-human animal and inducing differentiation to a tissue or organ in which a vascular network has been constructed. A method for evaluating drugs using at least one selected from a group consisting of the biological tissue, tissues and organs produced by said method, and non-human chimera animals produced by said method. A regenerative therapy composition comprising biological tissue in which a vascular system has been provided by the method.

The invention discloses a biocompatible miniature frictional generator. The biocompatible miniature frictional generator consists of an upper pole plate nylon 66 coating layer, an upper pole plate poly-L-lactic acid macromolecule pair frictional layer, a lower pole plate ethyl cellulose pair frictional layer and a lower pole plate nylon 66 coating layer, wherein an upper pole plate material is an insulation type plate material formed by poly-L-lactic acid, and electrons are easily obtained; a lower pole plate is an insulation type plate material formed by ethyl cellulose, and electrons are easily lost; patterns in different shapes are manufactured on contact interfaces of the upper and lower pole plates, the upper and lower pole plates are in tight contact in a concave-convex opposite manner to form an arc-shaped space, and the space is packaged by a biocompatible nylon 66 film. The biocompatible miniature frictional generator has the advantages that the miniature generator is simple in structure, small in size and easy to operate; after the generator is inserted in vivo, micro voltage and micro current are generated by extrusion friction such as organism exercise, external massage and vibration; the generator can serve as a power supply of miniature diagnosis and treatment equipment implanted in vivo, and is widely applied to the medical fields of diagnosis, quick treatment, organ regeneration and the like.

The invention discloses a medicine for accelerating the repair of stratum epidermis and the regeneration of skin appendages. The medicine consists of epidermal stem cells, bone marrow mesenchymal stem cells and a medicine carrier, wherein the epidermal stem cells and the bone marrow mesenchymal stem cells are adhered to the medicine carrier, namely each cubic millimeter of the medicine carrier contains (0.3-1)*104 epidermal stem cells and (0.3-1)*104 bone marrow mesenchymal stem cells. The medicine has an obvious effect in aspects of promoting the regeneration of the stratum epidermis, promoting the regeneration of dermis, enhancing the mechanical strength of regenerated skin, promoting the content of collagen of the regenerated skin, promoting revascularization, promoting the regeneration of hair follicles and the like, so that wounds can be repaired within a short time and the regeneration of the skin appendages can be accelerated. The invention also discloses a method for preparing the medicine for accelerating the repair of the stratum epidermis and the regeneration of the skin appendages, and the method is simple, high in controllability, high in operability and high in repeatability.

Pluripotent human embryonic stem cells (hESCs) hold great potential for restoring tissue and organ function, which has been hindered by inefficiency and instability of generating desired cell types through multi-lineage differentiation. This instant invention is based on the discovery that pluripotent hESCs maintained under defined culture conditions can be uniformly converted into a specific lineage by small molecule induction. Retinoic acid induces specification of neuroectoderm direct from the pluripotent state of hESCs and triggers progression to neuronal progenitors and neurons efficiently. Similarly, nicotinamide induces specification of cardiomesoderm direct from the pluripotent state of hESCs and triggers progression to cardiac precursors and cardiomyocytes efficiently. This technology provides a large supply of clinically-suitable human neuronal or cardiac therapeutic products for CNS or myocardium repair. This invention enables well-controlled efficient induction of pluripotent hESCs exclusively to a specific clinically-relevant lineage for tissue and organ engineering and regeneration, cell-based therapy, and drug discovery.

The instant invention provides, for the first time, the use of cadaveric organs from donors with non-beating hearts as a source of functional cells such as progenitor or stem cells for various medical purposes. More specifically, a method is disclosed whereby a tissue source of progenitor cells is obtained comprising harvesting tissue from a donor, wherein the donor has a non-beating heart for as long as about thirty hours postmortem and processing the cadaveric tissue to provide progenitor cells. The instant progenitors are used for various medical purposes as means of cell therapy, gene therapy, artificial organs, bioreactors, organ regeneration and the like.

The invention discloses a zebrafish nervous tissue-specific enhancer and cloning and application thereof and relates to the technical field of acquisition of enhancers for tissue and organ specific expressed genes. The enhancer is used for fluorescently labeling nerve cells in a zebrafish embryo development process. Sequences of the enhancer are nucleotide sequences shown in SEQ ID NO. 2. The application of the enhancer includes: developing transgenic fishes of nervous tissue-specific expressed fluorescence so as to serve for studying nerve and organ regeneration; driving specific expression of any target genes in nervous tissues so as to provide powerful tools for researchers to study development of nervous system and treatment of related diseases. The enhancer is applicable to the research on zebrafish eye development and injury regeneration processes, helps study the effect of specific genes in terms of nervous system development and controlling and provides new materials for the treatment of related diseases.

An object of the present invention is to provide a method which constantly enables organ regeneration for the purpose of achieving organ regeneration with higher efficiency. It has been discovered that, in a blastocyst complementation method, a next generation is born when a deficiency in an organ, such as pancreas and kidney, is complemented by injection of ES cells into a generated blastocyst, and further discovered that a transgenic animal having a pancreas or a kidney thus complemented can transmit the phenotype to the next generation as a founder. This discovery has revealed that organ regeneration can be accomplished by using such a founder. Thus, the present invention achieved the above-described object.

The invention provides an economic, simple and convenient lung organ culture method and an experimental model, and relates to the technical field of biology. The method comprises the following steps: marking a transgenic mouse with pulmonary epithelial cells by using GFP (Green Fluorescent Protein), sorting the pulmonary epithelial cells and interstitial cells by using a flow cytometry, then co-culturing the sorted lung epithelial cells and mesenchymal cells in an upper-layer small chamber and a lower-layer small chamber divided by Transwell, and promoting the epithelial cells in the upper-layer small chamber to grow and develop into lung organs by using growth factors and biological components secreted by the mesenchymal cells in the lower-layer small chamber. According to the method of the invention, the natural growth environment and mode of in-vivo cells are simulated, and the lung bronchial organ can be obtained under the condition that exogenous growth factors are not added. The mouse lung organ can be used as the experimental model, and can be used for research on lung development process, cell differentiation, lung toxicology, drug screening, organ regeneration and transplantation, etc.

Mechanisms nourish stem cells for organ regeneration and prevent alcohol related diseases such as Fetal Alcohol Syndrome (FAS) and Liver Sclerosis. These stem cell nutrients have been found to positively affect the skin, liver, brain neurons, pancreas, and the GI tract. Cholesterol supplementation prevents fetal alcohol spectrum defects (FASD) in alcohol-exposed zebra fish embryos. Using the zebra fish model, alcohol was found to interfere with embryonic development by disrupting cholesterol-dependent activation of a critical signaling molecule, sonic hedgehog (Shh). Cholesterol supplementation of the alcohol-exposed embryos restored the functionality of the molecular pathway and prevented development of FASD-like defects. Novel biomarkers were identified for diagnosing alcohol related diseases by lipid chemical analysis and Raman Spectroscope.