29 results about "Cellular composition" patented technology

Methods and systems for analysing the cellular composition of a sample are described, comprising: providing an image of the sample in which a plurality of cellular populations are associated with respective signals and classifying a plurality of query cells in the image between a plurality of classes corresponding to respective cellular populations in the plurality of cellular populations. This is performed by providing a query single cell image to an encoder module of a machine learning model to produce a feature vector for the query image, and assigning the query cell to one of the plurality of classes based on the feature vector for the query image and feature vectors produced by the encoder module for each of a plurality of reference single cell images. The machine leaning model comprises: the encoder module, configured to take as input a single cell image and to produce as output a feature vector the single cell image, and a similarity module configured to take as input a pair of feature vectors for a pair of single cell images and to produce as output a score indicative of the similarity between the single cell images. Thus, the machine learning model can be obtained without the need for an extensively annotated dataset. The methods find use in the analysis of multiplex immunohistochemistry / immunofluorescence in a variety of clinical contexts.

The present application relates to apparatus and methods for cellular composition. Embodiments of the invention provide systems, methods and apparatus for real-time cellular composition / generation of music using cells (i.e. short musical motifs), wherein the cellular composition relies on user data.

The invention provides a spatial transcriptome cell composition inference method based on graph contrast learning. According to the method, a cross-modal low-dimensional feature spatial data simulation module is constructed, single cell and spatial transcriptome data are respectively mapped to a potential embedding space, feature space difference is reduced through aligned distribution, and structural consistency of cross-modal data is enhanced; designing a double heterogeneous graph construction and potential relation learning module, constructing a feature heterogeneous graph of two cells and spatial points based on gene expression similarity, and capturing a high-level potential relation of spatial data in double graphs by adopting a meta-path potential relation reasoning strategy; and introducing a graph representation optimization mechanism based on structure contrast learning, maximizing the consistency of the cross-modal nodes in the embedding space, and obtaining a final cell composition inference result. The method realizes inference of spatial transcriptome data cell composition, can be used for cell space positioning and tissue microenvironment analysis, and provides a reliable computational analysis basis for related biomedical research.

The invention discloses a construction method and application of a cerebellar organoid model, and belongs to the technical field of organoid culture. According to the method, the stability and repeatability of cerebellar organs are remarkably improved by optimizing the components of the culture medium and the induction process. Key morphogenetic factors are regulated and controlled in a stepped mode, and cells are accurately guided to be directionally differentiated to cerebellum; meanwhile, a culture medium without Matrigel is used in the nerve expansion and maturation stages, batch variation of exogenous matrixes is eliminated, and development path consistency and cell composition uniformity are ensured. The organoid constructed on the basis of the method has remarkable advantages in SCA3 disease modeling, single cell sequencing proves that the difference in batches is remarkably smaller than that of an existing method, and a reliable in-vitro human model platform is provided for SCA3 mechanism research and drug screening.

Compositions comprising a population of oligodendrocyte progenitor cells (OPCs), as well as methods of making and using the same, are provided. In one aspect, a container comprising a composition, where the composition comprises a population of cells comprising a plurality of OPCs, and where the population of cells comprises less than 15% undesirable cell types is provided. In another aspect, the population of cells comprises less than 15% undesirable epithelial lineage cells. In yet another aspect, the population of cells comprises less than 2% K7 positive cells. In an aspect, a population of cells comprising a plurality of oligodendrocyte progenitor cells is capable of forming less than one epithelial cyst per 100,000 cells in a cyst assay is provided. An even further aspect of the present disclosure is a container comprising a composition, where the composition comprising a plurality of oligodendrocyte progenitor cells is useful in treating treat stroke, spinal cord injury, and multiple sclerosis.

The present invention provides, in some embodiments, isolated cell compositions suitable for adoptive immunotherapy, methods for making the cell compositions, and therapeutic methods using the cell compositions. The compositions comprise at least about 10 mAbs specific for a target peptide antigen in a pharmaceutically acceptable carrier. 6 The compositions comprise CD8+ T cells and include T memory stem (TSCM) cells. In various embodiments, the compositions are about 1% to about 100% T memory stem cells, providing robust and durable adoptive therapy and providing advances in T cell engineering.

The present disclosure relates to a method of reducing urine glycosaminoglycans (GAG) or cerebral spinal fluid (CSF) glycosaminoglycans (GAG) in a subject, comprising: administering a single dose of a population of modified B cells to the subject.

The invention relates to a cell composition and a cell dressing for repairing skin injury and regenerating hair follicles and application of the cell composition and the cell dressing, active cells of the cell composition comprise hair papilla cells, keratinocytes and fibroblasts, and the number ratio of the hair papilla cells to the keratinocytes to the fibroblasts is (1-3): (1-3): (1-3). The cell material provided by the invention can treat or repair skin injury, especially head skin injury, and can promote hair regeneration while repairing the skin injury.

This invention relates to an in vitro method for mapping the spatiotemporal electrophysiological dynamics and spatial transcriptional profiles of cells in a functional neuronal cell ensemble. This in vitro method includes simultaneously recording and analyzing spatial data of molecular activity and electrical network activity at the level down to individual cells using high-density electrobiosensors, spatial transcriptomics, optical imaging, and advanced computational strategies. The invention also relates to: methods for identifying the composition of a functional neuronal ensemble; methods for monitoring the spatiotemporal electrophysiological dynamics and transcriptional profiles of cells in a functional neuronal cell ensemble; methods for monitoring the cellular composition of a functional neuronal ensemble; methods for identifying compounds that influence spatial electrophysiological and transcriptional dynamics; and / or methods for identifying the cellular composition of a functional neuronal cell ensemble based on detected dynamics; and devices thereof. This invention allows for a better understanding of disease mechanisms, the identification of therapeutic targets, and the development of new drugs and treatment regimens.

Provided herein are methods of hypoimmunogenicity, such as bioengineering methodologies and materials, including hypoimmunogenicity (such as engineering hypoimmunogenicity) methodologies and materials useful in, for example, genetically modifying and / or otherwise altering at least one target gene or gene product, processes for producing engineered hypoimmunogenic cells, manufacturing of engineered hypoimmunogenic cellular compositions, and uses thereof.

The present disclosure relates to improved cell compositions and potency assays for obtaining same, which may be suitable for use in connection with T-cell mediated diseases such as graft-versus-host disease (GvHD).

Nucleic acid molecules comprising truncated forms of the human cytomegalovirus (CMV) promoter are operably linked to a transgene of interest, including those encoding eNOS protein are taught. Vectors comprising these nucleic acid molecules and host cells transformed by these vectors and methods of producing cellular compositions are used for the treatment of a variety of pulmonary and cardiac diseases. There is provided a truncated human cytomegalovirus (CMV) enhancer element comprising SEQ ID NO: 1 or a functional derivative thereof. A truncated human cytomegalovirus (CMV) promoter comprising the truncated CMV enhancer element is taught. A polynucleotide expression cassette comprising the truncated CMV promoter and a transcribable polynucleotide operably linked to the truncated CMV promoter polynucleotide construct, a host cell comprising the polynucleotide expression cassette, and use of the host cell treat renal, vascular, pulmonary, or cardiac disease in the patient are taught.

This disclosure provides a potency assay for evaluating the potency of facilitating cells and / or alpha beta TCR+ T cells.

The invention relates to a construction method of a human haploid blastoid, which comprises the following steps: firstly, sorting haploid cells, and placing the haploid cells in a human blastoid induction system to generate a human haploid blastoid-like structure with haploid attributes, namely the human haploid blastoid; the constructed human haploid blastoid has a structure and cell composition similar to those of a human blastocyst, and human embryonic stem cell lines, human trophoblast stem cell lines and human extraembryonic endoderm stem cell lines can also be established in different cell induction culture solutions. The human haploid blastoid has the structure and function similar to those of a human blastocyst, also has the advantages of haploid cells, and can be used for research on generation of human haploid embryo related cell lines, genetic screening of human early embryonic development and the like.

This application discloses a method, apparatus, electronic device, and readable storage medium for detecting the cellular composition of biological samples, applicable to the field of biomedical technology. The method includes performing single-cell transcriptome sequencing on the biological sample to be tested to obtain single-cell sequencing results; generating a cell gene expression matrix by analyzing the single-cell sequencing results; and determining the cell types contained in the biological sample by performing single-cell bioinformatics analysis on the cell gene expression matrix. This application enables low-cost, high-throughput, accurate, and quantitative one-step detection of the cellular composition of biological samples.

Provided herein are compositions of anorectal transition zone stem cells, to treat endodermal, ectodermal or mesodermal disease. Also provided are cellular compositions of the anorectal transition zone stem cells, in a pharmaceutically acceptable carrier.

Provided are methods for assessing exosomes produced from cells of a cell composition, such as a cell therapy composition, including compositions comprising chimeric antigen receptor (CAR) or T cell receptor (TCR) expressing T cells. In some embodiments, the methods can be used for predicting response to and / or efficacy of a cell therapy composition, for selecting patients for treatment, and in connection with T cell manufacturing methods.

The invention provides a method for evaluating drug lung injury in vitro through a lung organ model, and relates to the technical field of biological medicine. Comprising the following steps: preparing five different culture mediums, culturing the hESC by using the different culture mediums, inducing the hESC to respectively carry out directional endoderm cell differentiation, anterior intestinal bulb differentiation and lung organ construction, and evaluating the drug lung injury by using a prepared lung organ model. The lung organ model prepared by the invention is similar to the cell composition of the human lung organ, can accurately reflect the toxic effect of the medicine on the lung in the physiological environment in the human body, and can be repeatedly obtained in quantity, so that clinical risks and ethical constraints are avoided; the sensitivity of the lung to different drugs can be reflected through cell DNA injury, and the method is suitable for in-vitro repeatable and high-throughput rapid evaluation of drug lung injury.

Belts and fibrous structures including discrete cells having one or more legs and / or one or more concavities in certain patterns or Cell Groups. The cells may be discrete knuckles or pillows and the fibrous structures may further include an emboss.

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, and also allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

Provided herein are methods for multiplexed in situ analysis of biomolecules in a tissue. In particular, provided herein are methods for multiplexed single-cell in situ protein and nucleic acid profiling in fixed or fresh tissues, that allows the investigation of the different cell compositions and their spatial organizations in intact tissues through consecutive cycles of probe hybridization, fluorescence imaging, and signal removal.

The invention provides a method for induced differentiation of human pluripotent stem cells into functional intestinal organoid, and belongs to the field of stem cell technology and organoid engineering. The core of the invention is to provide a set of culture medium formula system with definite components and clear stages, and the intestinal organs can be efficiently guided to differentiate to various functional cell lineages including epithelial cells, neuroendocrine cells and endothelial cells, so that mature organs highly simulating natural intestinal tracts in cell composition are formed. Through a systematized culture medium formula, a standardized operation process and a multi-stage time sequence induction strategy, the next-generation 3D intestinal organ with structural integrity, cell diversity and functional activity at the same time is successfully constructed. The invention provides an efficient, reliable, repeatable and easy-to-expand advanced in-vitro model for intestinal development, disease mechanism, drug screening, toxicity evaluation and regenerative medicine research, and has important scientific research and transformation application value.

Methods and compositions relating to at least partially recellularized human organs are provided herein. Various methods for decellularizing non-human animal organs and recellularizing the non-human animal extracellular matrix with cellular compositions. Furthermore, compositions and methods for treating liver disease or other diseases (such as acute liver failure) using an extracorporeal bioartificial liver or other organs are provided herein.

The present invention related to a compound for use in prevention or treatment of a neurodegenerative disease associated with the formation of stress granules. The compound is selected from lipoic acid, lipoamide, dihydrolipoic acid, and dihydrolipoamide. The invention further relates to a method of identifying a compound that modulates a characteristic associated with one or more condensates comprising a condensate-associated molecule, comprising: (a) contacting the compound with a cellular composition comprising one or more condensates or a cellular composition capable of forming one or more condensates, and (b) determining the characteristic associated with the one or more condensates. A modulation in the characteristic, as compared to a reference, indicates that the compound modulates the characteristic associated with the one or more condensates.

The present disclosure relates to a method of reducing urine glycosaminoglycans (GAG) or cerebral spinal fluid (CSF) glycosaminoglycans (GAG) in a subject, comprising: administering a single dose of a population of modified B cells to the subject.

This invention relates to a method for the rapid and large-scale preparation of liver organoids, and to methods for screening drugs related to liver diseases and screening drug in vitro toxicity using these methods. According to the above-described preparation method, liver organoids can be rapidly and massively prepared by continuously undergoing a three-dimensional differentiation process on the same microplate. The massively prepared liver organoids using the above-described method include hepatocytes (solid core part), cholangiocytes (cystic part), hepatic stellate cells, and Kupffer cells, possessing a cellular composition, structure, and function similar to actual liver tissue, and can stably proliferate in vitro. Furthermore, the in vitro drug toxicity screening method for liver organoids of this invention allows for the pre-screening of drugs that may have hepatotoxicity, shortening the time and cost of new drug development and improving its efficiency.

The present disclosure relates to cellular compositions that are modified to introduce a nucleic acid or vector expressing the same. Such compositions may be used to deliver nucleic acid to a target cell and treat disease such as cancer.

Provided herein are methods of hypoimmunogenicity, such as bioengineering methodologies and materials, including hypoimmunogenicity (such as engineering hypoimmunogenicity) methodologies and materials useful in, for example, genetically modifying and / or otherwise altering at least one target gene or gene product, processes for producing hypoimmunogenic cells (such as engineered hypoimmunogenic cells), manufacturing of hypoimmunogenic cellular compositions (such as engineered hypoimmunogenic cellular compositions), hypoimmunogenic cell systems (such as engineered hypoimmunogenic cell systems) and uses thereof.

The present application discloses a biological sample cell composition detection method and apparatus, an electronic device, and a readable storage medium, which are applied to the technical field of biomedicine. The method comprises: performing single-cell transcriptome sequencing on a biological sample to be detected to obtain a single-cell sequencing result, generating a cell gene expression matrix by analyzing the single-cell sequencing result, and performing single-cell bioinformatics analysis on the cell gene expression matrix to determine cell types comprised in said biological sample. The present application can accurately and quantitative detect the cell composition of a biological sample in one step with low cost and high throughput