37 results about "Tumor biopsy" patented technology

The invention relates to the technical field of biopsy puncture, and discloses a tumor biopsy puncture device for biopsy sampling, the tumor biopsy puncture device comprises an outer needle assembly and an inner needle assembly, the outer needle assembly comprises a needle base and an outer needle tube fixed with the needle base, the inner needle assembly comprises a needle cylinder and an inner needle core slidably arranged in the needle cylinder, a needle cavity is arranged at the needle tip end of the inner needle core, and the needle cavity is communicated with the needle base. An ejection mechanism is arranged in the needle cylinder, the ejection mechanism is used for triggering the inner needle core to eject outwards in the length direction of the outer needle tube, and the ejection mechanism comprises a connecting part fixed to the inner needle core, a locking piece arranged on the connecting part and used for limiting the position of the inner needle core and a pull rod fixed to the connecting part and capable of penetrating out of the needle cylinder in a sliding mode. According to the tumor biopsy puncture equipment for biopsy sampling, by arranging the ejection mechanism, compared with an existing manual operation mode, the ejection force of the inner needle core is adjusted by changing the stretching or compression degree of the elastic component, and the cutting requirements of tumor tissues of different textures can be met.

The invention discloses a multi-modal image fusion assisted precise tumor needle biopsy guide system, and aims to solve the problem that the traditional needle biopsy guide technology is insufficient in precision. The system innovatively adopts a multi-scale feature fusion algorithm and an adaptive weight fusion strategy, deeply fuses ultrasonic, CT, MRI and other multi-modal images, and comprehensively improves the information amount of the images. Through real-time force feedback and path adjustment, in combination with VR / AR guide interaction, intelligent puncture guide is realized, and safe and accurate operation is guaranteed. Tumor features are extracted by using a deep learning algorithm, accurate tumor recognition and dynamic image analysis are realized, and the tumor growth trend is predicted. According to the system, the precision and success rate of needle biopsy are remarkably improved, the pain of a patient and the medical cost are reduced, powerful support is provided for personalized precise treatment of tumors, and the system has extremely high clinical application value.

The invention discloses a medical tumor biopsy device, relates to the technical field of biopsy sampling equipment, and aims to provide the medical tumor biopsy device which adopts a rotary cutting mode, avoids pop-up tumor penetration and reduces the operation difficulty. A rotary needle tube is arranged in the shell, a fixed needle tube is arranged on the outer side of the rotary needle tube in a sleeving manner, the fixed needle tube is arranged below the shell, a rotary driving assembly connected with the rotary needle tube is arranged in the shell, and a coil spring connected with the interior of the shell is arranged on the rotary needle tube in a sleeving manner. The inner tube body can be driven by the rotary driving assembly to rotate by 180 degrees, so that the chamber of the sampling cavity is aligned with the sampling opening, the tumor tissue enters the sampling cavity at the moment, then the inner tube body rotates by 180 degrees again, the tumor tissue is cut off through the cutter on the side face, and the tumor tissue is sealed in the sampling cavity and the sampling opening at the moment.

The present application relates to the technical field of clinical medicine. Disclosed is a medical clinical tumor biopsy sampling apparatus, comprising a main body (1). A sampling tube (2) is fixedly connected to the front end of the main body (1). A positioning mechanism is arranged outside the sampling tube (2). A driving plate (3) is slidably connected to the interior of the main body (1). Pull rings (4) are fixedly connected to the left and right ends of the driving plate (3). A clamping block (5) is fixedly connected to the front end of the driving plate (3). Two clamping rings (6) are arranged in the main body (1). Pressing plates (7) are fixedly connected to opposite ends of the two clamping rings (6). Springs (a8) are fixedly connected to opposite ends of the two clamping rings (6). The ends of the two springs (a8) away from the clamping rings (6) are fixedly connected to the inner wall of the main body (1). By means of the structures of the sampling tube (2), a puncture tube (10), a sealing rod (13), and the like, the functions of a traditional puncture device and a sampling device are superimposed, the sampling operation can be directly carried out by one device, and the device has a simple structure, thereby improving the practicability of the device.

The invention discloses a medical clinical tumor biopsy sampling device which comprises a sampling mantle fiber, one end of the sampling mantle fiber is fixedly connected with a tail end connecting cylinder, the other end of the sampling mantle fiber is fixedly connected with a storage sliding sleeve, a rotating guide wire is slidably mounted in the sampling mantle fiber, a sliding piston is slidably mounted in the storage sliding sleeve, and the rotating guide wire is fixedly connected with the storage sliding sleeve. A threaded sampling cone is fixedly connected to one side of the sliding piston, the other side of the sliding piston is fixedly connected with a rotating guide wire, a sampling groove is formed in the surface of the threaded sampling cone, scraping teeth are fixedly connected into the sampling groove, a connecting plug is fixedly connected to one side of the tail end connecting cylinder, and the connecting plug is communicated with the interior of the tail end connecting cylinder. Certain air is blown into or pumped out of the tail end connecting cylinder, the air is conducted to the storage sliding sleeve through the sampling mantle fiber to push the sliding piston to move, then the threaded sampling cone is controlled to stretch out and retract, and the situation that when a sampling groove passes through the gastroscope, the interior of the gastroscope is damaged is avoided.

The invention discloses a digestive tract tumor biopsy sampling device convenient for puncture and sampling for medical oncology, which relates to the technical field of tumor biopsy sampling and comprises a biopsy device, a limiting seat and a base. According to the alimentary canal tumor biopsy sampling device convenient to puncture and sample for the medical oncology, tumor tissue can be accurately contained through the sampling groove, fragmentation of a tumor tissue sample is avoided, it is guaranteed that the tissue sample meets the tissue form needed by detection, the sample quality is guaranteed, the outer sleeve needle covers the sampling groove after sampling, the sample can be fixed and cannot fall off, and the sampling efficiency is improved. The tissue is prevented from remaining in the body or being polluted during withdrawing, and meanwhile the risks of bleeding and tissue damage in the puncture process are reduced; enough samples can be obtained through one-time operation, the repeated puncture frequency is reduced, the operation time is shortened, the pain of a patient is relieved, and the operation efficiency is improved; and the modularized puncture needle design allows medical staff to quickly adjust the puncture depth according to individual differences, and the flexibility is high.

Immunohistochemistry (INC) and in situ hybridization (ISH) methods are used for the targeted detection and localization of biomolecules (e.g., proteins and miRNAs) in tissues or cells, for research applications and clinical applications such as those of pathologists (e.g., biomarker analysis of resected tumors or tumor biopsies). These methods include mass spectrometry imaging (MSI) as a mode for detecting and localizing biomolecules in tissues or cells. The methods employ optically cleavable mass-tagged reagents linked to probes such as antibodies and nucleic acids and used to enable multiplexed immunohistochemistry and in situ hybridization, with MSI as the detection / readout mode. The methods also include multi-omics MSI procedures in which MSI of optically cleavable mass-tagged probes is combined with other MSI modes, such as direct label-free MSI of endogenous biomolecules from biological samples (e.g., tissues).

Methods, devices and systems are provided to analyze the immunologic response of patients at various times before, during and / or after immunomodulating therapy, particularly treatment with specialized pulsed electric field energy, in some embodiments, such methods, devices and systems include a laboratory developed test that utilizes a patient tumor biopsy sample and a series of blood draws to determine whether the locoregional therapy (with or without additional systemic therapies, or systemic therapies-alone) was sufficient to facilitate an increase in tumor-specific antibody production. In some embodiments, the focus is on evaluation of baseline antibody levels, prior to any treatment, and changes in levels of antibodies to given antigens, especially in response to a given therapy treatment. The treatment, such as delivery of specialized FEF energy to a target tissue, is then performed and antibody levels are checked at one or more later timepoints to detect changes in the presence of antibodies and therefore response to therapy.

The invention relates to the technical field of sampling devices, and discloses a medical clinical tumor biopsy sampling device which comprises a sleeve and further comprises a sampling part arranged on the sleeve; the lifting part is arranged in the sleeve; the limiting part is arranged at the top of the sleeve; the sampling part comprises a sampling assembly; the sampling assembly is mounted on the sleeve; the rotary cutting assembly is mounted on the sleeve; the sampling assembly comprises two sampling openings formed in the outer wall of the sleeve, the bottom end of the sleeve is fixedly connected with a puncture head, the outer wall of the puncture head is fixedly connected with an umbrella-shaped annular knife, and a sampling bin is formed in the top of the puncture head. According to the tumor biopsy sampling device, by arranging the sampling part, the problems that according to an existing tumor biopsy sampling device, a sample is not prone to remaining on the sampling device, the sample falls off, the accuracy and timeliness of follow-up pathological detection are affected, and finally the overall working efficiency of clinical biopsy is reduced are solved.

The present invention belongs to the technical field of immune cell culture, and specifically relates to a composition and kit for expanding tumor infiltrating lymphocytes, and an expansion method therefor. Provided is a composition for expanding tumor infiltrating lymphocytes for a trace amount of tumor tissue, i.e., a tumor biopsy sample, to perform the expansion of tumor infiltrating lymphocytes, which solves the problem that a large amount of a tumor tissue sample is required for tumor infiltrating lymphocytes, and is suitable for patients who cannot undergo surgical resection to obtain tumor tissue. In addition, by means of optimizing the combination and working concentration of antibody stimulants, a process flow for expanding tumor infiltrating lymphocytes on the basis of biopsy samples is established, such that the stemness of the expanded tumor infiltrating lymphocytes is maintained, and the killing ability is improved, thereby ensuring the anti-tumor efficacy after reinfusion into the body.

The utility model relates to the technical field of biopsy, in particular to cold storage equipment for tumor biopsy samples. The technical problem to be solved is to provide a refrigeration storage device capable of independently storing tumor biopsy samples. According to the technical scheme, the refrigeration storage equipment for the tumor biopsy samples comprises a bottom frame and the like. The upper end of the bottom frame is fixedly connected with a middle frame, the upper sides of the left end and the right end of the middle frame are rotationally connected with symmetrical turning covers, the middles of the front end and the rear end of each turning cover are rotationally connected with symmetrical connecting hole plates, and the left sides and the right sides of the symmetrical connecting hole plates are rotationally connected to the upper sides of the front end and the rear end of the middle frame. The front and rear sides of the left end of the placing box are rotationally connected with small rotating plates. When people carry out cold storage on tumor biopsy samples, firstly, a plurality of placing boxes with a sealing function are arranged, and an independent package is formed by the plurality of placing boxes.

This invention discloses an intelligent digestive tract tumor biopsy sampler and system, belonging to the field of medical device technology. It includes a puncture cone, shaft, end cap, annular blade, motor, and lead screw. A cutting opening is formed between two annular walls. The motor drives the lead screw to move the annular blade up and down for sampling. The device is equipped with stepless speed control buttons, a stroke limit structure, status indicator lights, a negative pressure adsorption component, and an ergonomic non-slip handle. This invention integrates a pressure sensor, an AI module, a storage module, and a wireless transmission module, allowing connection to remote devices. The device supports both manual and intelligent working modes. It relies on the pressure sensor to collect cutting resistance in real time, and the AI ​​adaptively adjusts the cutting speed and stroke. It features fault self-diagnosis, audible and visual prompts, remote parameter updates, and algorithm upgrades. This invention has a compact structure, can seal and preserve samples, produces a regular cutting surface, effectively prevents sample detachment and contamination, reduces reliance on operational experience, and improves sampling accuracy and surgical safety.

The application discloses a kind of negative pressure suction gun, manual negative pressure suction method, it is related to biomedical engineering technical field, the gun handle of negative pressure suction gun is reciprocated and opened and closed to drive suction pumping section to reciprocate in negative pressure sedimentation cavity Negative pressure suction and extrusion pumping, negative pressure sedimentation cavity can pass through liquid suction pipe one-way liquid suction when negative pressure suction, negative pressure sedimentation cavity can be one-way liquid delivery to the liquid storage part when extrusion pumping, so as to be used for suction liquid and soft solid tissue in liquid by setting negative pressure sedimentation cavity, then by adjusting increase negative pressure suction force, the viscosity of solid tissue that can be sucked is also greater, so that the negative pressure suction gun of the application can not only be used for endometrial gynecological field liquid suction and solid tissue sampling, but also can be used for closed narrow space tumor biopsy, brain tissue and other solid-liquid components suction, during suction process, by rotating liquid suction pipe, the inlet slope of liquid suction pipe is realized 360 degree rotation, to avoid suction dead angle.

The present disclosure provides a method for monitoring residual disease in a subject. Generally, the method comprises determining a frequency of cancer mutations from a first sample obtained from a tumor biopsy from the subject; determining a frequency of the cancer mutations discovered in the first sample from a second sample comprising cell-free deoxyribonucleic acid (cfDNA) molecules that is obtained from the subject after the subject has undergone a course of treatment for cancer; and determining a presence or absence of cancer in the subject based on an analysis of the frequency of the cancer mutations from sequence data from the second sample.

The invention belongs to the technical field of immune cell culture, and particularly relates to a tumor infiltration lymphocyte amplification composition, a kit and an amplification method thereof. The invention provides the tumor infiltration lymphocyte amplification composition aiming at a trace amount of tumor tissue, namely a tumor puncture sample, the tumor infiltration lymphocyte amplification composition is used for amplifying the tumor infiltration lymphocyte, the problem that the tumor tissue sample size required by the tumor infiltration lymphocyte is large is solved, and the tumor infiltration lymphocyte amplification composition is suitable for a patient who cannot receive the tumor tissue obtained by surgical excision. Besides, by optimizing the antibody stimulant combination and the working concentration and establishing the technological process for amplifying the tumor infiltration lymphocytes based on the puncture sample, the dryness of the tumor infiltration lymphocytes obtained through amplification is maintained, the lethality is improved, and therefore the anti-tumor curative effect after the tumor infiltration lymphocytes are infused back into the body is guaranteed.

A kind of skin scale for operation, mainly by the transverse scale plate and longitudinal scale plate connected with each other vertically, is connected with detachable bandage by connecting block.The wire mesh structure with adjustable spacing is arranged between scale plate, including transverse and longitudinal arrangement of metal wire.Scale plate surface is provided with accurate scale, to facilitate direct measurement.Longitudinal metal wire spacing is controlled by screw adjusting mechanism, and transverse metal wire adopts magnetic type sliding fixing device to realize quick positioning.Bandage realizes quick disassembly and assembly by buckle type connector.The present application is manufactured by medical grade material, with good biocompatibility and perspective development nature.The device solves the measurement error problem caused by body position change, skin stretching and other factors in traditional operation positioning, and can provide stable and reliable body surface positioning reference.It has simple structure, convenient operation, and can be widely applied to orthopedic surgery, tumor biopsy and other medical operations requiring accurate body surface positioning.

To provide a method for treating malignant tumors.SOLUTION: A method of treating a malignancy in a patient, the method comprising: (a) analyzing a tumor biopsy from the patient to characterize a tumor microenvironment; and (b) administering to the patient an effective dose of a T cell comprising one or more chimeric receptors, wherein the effective dose is determined using the characteristics of the tumor microenvironment.SELECTED DRAWING: Figure 1

The tumor biopsy sampling equipment comprises a handle, a puncture needle, a knob and a needle head, the puncture needle is in a hollow tube shape, the puncture needle is fixed at the lower end of the handle, the knob is inserted into the end of the handle, the lower end of the knob is fixedly connected with a connecting needle, the connecting needle penetrates through the handle and is inserted into the puncture needle, and the lower end of the connecting needle is fixedly connected with the needle head. The needle head penetrates out of a port of the puncture needle, and an elastic slice is arranged in the puncture needle. According to the tumor biopsy device, a tumor biopsy sample with a quantitative depth can be obtained by utilizing a direct puncture means, and meanwhile, the tumor biopsy sample is obtained in an automatic suction and cutting mode, so that the obtaining difficulty is reduced.

Provided are methods, compositions, recombinant DNA molecules, and kits for cloning T cell receptors (TCRs). The methods facilitate construction of TCR expression libraries from biological samples containing antigen-specific T cells, including but not limited to tumor biopsies, including frozen tumor biopsies. Peripheral T cells that were engineered with library-derived TCR genes show potent therapeutic anti-tumor effects. The method can be performed using any sample that contains T cells, and can be performed with oligoclonal populations of T cells, such as T cells that have infiltrated a tumor. Primer combinations for first strand cDNA synthesis, second strand cDNA synthesis, and for cloning a plurality of distinct TCR β and TCR α chains into a plurality of vectors are provided. Cells containing the vectors are provided, as are kits for use in rapid cloning of the TCR β and TCR α chains.

The field of this invention relates to immunohistochemistry (IHC) and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins and miRNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. More specifically, the field of this invention relates to photocleavable mass-tag reagents which are attached to probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry and in situ hybridization, with MSI as the mode of detection / readout. Probe types other than antibodies and nucleic acids are also covered in the field of invention, including but not limited to carbohydrate-binding proteins (e.g., lectins), receptors and ligands. Finally, the field of the invention also encompasses multi-omic MSI procedures, where MSI of photocleavable mass-tag probes is combined with other modes of MSI, such as direct label-free MSI of endogenous biomolecules from the biospecimen (e.g., tissue), whereby said biomolecules can be intact or digested (e.g., chemically digested or by enzyme).

The present application relates to the technical field of medical apparatus and instruments, in particular to a medical clinical tumor biopsy sampling device. The device comprises a shell, a flip cover rotatably connected to one side of the shell, a stop lever rotatably connected to the flip cover, a curved lever arranged on one side of the shell and limitedly matched with the stop lever, a hollow puncture needle fixedly connected to one side of the shell close to the flip cover, an inlaying opening arranged on the side of the hollow puncture needle away from the shell, a sampling needle core slidably and rotatably connected in the hollow puncture needle, and a sampling opening arranged on one side of the sampling needle core close to the inlaying opening. The sampling needle core is arranged in the hollow puncture needle, so that the interference area with the tumor during sampling is avoided from being too large, the tumor is prevented from being damaged and dispersedly transferred due to too large disturbance, the tumor sampling area is locally disturbed by rotating the hollow puncture needle, the influence on the tumor body is reduced, and the protection of the patient is enhanced.

Disclosed herein is a method for determining tumor margins relative to non-tumorous tissue using a deep-learning platform. Also disclosed herein are methods for removing a tumor from a tumor biopsy.

The rapid tumor sampling device comprises a lower cylinder body and an upper cylinder body, one side of the top of the upper cylinder body is communicated with an adsorption tube through an opening, a first ring and a second ring are fixedly arranged on the inner wall of the top of the adsorption tube, and a diaphragm is bonded to the inner wall of the corresponding side of the first ring and the second ring; a cutting wire is inserted into a gap between the first ring and the second ring, and the outer walls of the two ends of the cutting wire penetrate through one side of the top of the adsorption pipe to be fixedly connected with the outer wall of the top of the first sliding block. According to the device, through organic combination of directional cutting mechanical design and negative-pressure adsorption positioning, a quick, accurate and low-trauma acquisition mode of a tumor sample is realized, and the controllability of the cutting direction and the path stability are obviously superior to those of a traditional clamping or puncture needle rotary cutting technology; the device is especially suitable for scenes with strict requirements on operation space and precision, such as deep tumor biopsy, and an efficient and safe standardized sampling solution is provided for clinic.

The invention relates to the technical field of biopsy puncture outfits, and particularly discloses a tumor biopsy puncture outfit based on a nasal cavity and a urethra open lacuna, the tumor biopsy puncture outfit comprises a handpiece and a needle tube in threaded connection with the handpiece, the handpiece is provided with a through groove, a sliding groove is formed in the through groove, a sliding block is arranged in the through groove, and the sliding block is arranged in the sliding groove. The outer side of the sliding block is slidably connected with a sliding groove in the through groove through a protrusion, a hole penetrating through the two sides of the sliding block is formed in the sliding block, the sliding block is connected with a suction type cutting and sampling mechanism, the suction type cutting and sampling mechanism extends into the needle tube through a penetrating hole formed in the handheld part, and a sampling hole used for sampling is formed in the needle tube. The sliding block is further provided with a pneumatic limiting mechanism, and the pneumatic limiting mechanism comprises an air channel arranged in the sliding block. Only sample tissue at the tumor core position can be extracted, and during sampling, a cutting mode is adopted, so that the problem that in the prior art, negative pressure extraction is adopted, the internal tissue of a tumor is pulled, and consequently pain of a patient is caused can be solved.

The utility model discloses a digestive tract tumor biopsy sampling device which comprises a needle cylinder, a telescopic device is arranged in the needle cylinder, a sampling needle is arranged at the bottom of the telescopic device, the outer wall of the needle cylinder is fixedly connected with a fixing ring, the top of the fixing ring is fixedly connected with a fixing rod, and the fixing rod is fixedly connected with the needle cylinder. And the top of the fixing rod is fixedly connected with a fixing plate. According to the device, a first servo motor is started to rotate, two screw rods of a bidirectional threaded rod are driven to do linear motion in opposite directions, two groups of dilators are driven to get close to each other or get away from each other, the two groups of dilators are controlled to expand to proper designated positions, and when a tumor of a patient is sampled, tissues at the sampling position of the patient can be expanded; the sampling needle is driven by the telescopic device to move downwards, so that the tumor tissue of a patient is penetrated, meanwhile, tumor tissue fluid can be sucked for biopsy sampling, and the use is flexible and convenient.

Methods, devices and systems are provided to analyze the immunologic response of patients at various times before, during and / or after immunomodulating therapy, particularly treatment with specialized pulsed electric field energy. In some embodiments, such methods, devices and systems include a laboratory developed test that utilizes a patient tumor biopsy sample and a series of blood draws to determine whether the locoregional therapy (with or without additional systemic therapies, or systemic therapies-alone) was sufficient to facilitate an increase in tumor-specific antibody production. In some embodiments, the focus is on evaluation of baseline antibody levels, prior to any treatment, and changes in levels of antibodies to given antigens, especially in response to a given therapy treatment. In such embodiments, this antibody testing is performed prior to treatment. The treatment, such as delivery of specialized PEF energy to a target tissue, is then performed and antibody levels are checked at one or more later timepoints to detect changes in the presence of antibodies and therefore response to therapy.

The field of this invention relates to immunohistochemistry (IHC), immunocytochemistry and in situ hybridization (ISH) for the targeted detection and mapping of biomolecules (e.g., proteins, glycans and RNAs) in tissues or cells for example, for research use and for clinical use such by pathologists (e.g., biomarker analyses of a resected tumor or tumor biopsy). In particular, the use of mass spectrometric imaging (MSI) as a mode to detect and map the biomolecules in tissues or cells for example. More specifically, the field of this invention relates to photocleavable mass-tag reagents which are attached to Probes such as antibodies and nucleic acids and used to achieve multiplex immunohistochemistry, immunocytochemistry and in situ hybridization, with mass spectrometry (MS) and mass spectrometry imaging (MSI) as the mode of detection / readout.

The disclosure relates to methods of identifying a unique cell type in a tumor biopsy sample based upon gene expression, and uses thereof for prognosing patients with melanoma.

The utility model discloses a bone tumor biopsy puncture needle convenient to use, which comprises a biopsy outer tube, a puncture needle tube and an extension tube body, the puncture needle tube comprises a tube needle body at the front end and a handle body at the rear end, the tube needle body is detachably inserted and fixed on the handle body, the two ends of the tube needle body are respectively provided with a puncture tip and a self-tapping drill bit, and the extension tube body is fixed on the extension tube body. An inserting clamping piece is arranged in the middle of the tubular needle body, a clamping hole is formed in the front end of the handle body, the inserting clamping piece is inserted and fixed to the clamping hole in a matched mode, and the portion, on one side of the tubular needle body, of the tubular needle body can be inserted into the handle body in a matched mode. By means of the bone tumor specimen taking device, bone tumor specimens can be taken by puncturing the bone surface in a puncturing mode, the bone surface can be penetrated in a self-tapping drilling mode, the bone tumor specimens can be smoothly obtained, the using function of the puncture needle is more flexible and practical, the working difficulty of doctors is lowered, and the using efficiency is improved.