123 results about "Recombinant DNA" patented technology

Provided are compositions for genome editing and site-directed integration in plants comprising microprojectile particles coated, treated of applied with a recombinant DNA construct comprising a sequence encoding one or more genome editing reagents for delivery to a mature embryo explant from dry seeds. Further provided are methods for genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, and plants, plant parts and seeds comprising an edited genome or site-directed integration, which are produced by the disclosed methods.

A transgenic soybean event, Gm_CSM63717, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gm_CSM63717 are also provided. Recombinant DNA molecules unique to the event Gm_CSM63717, and methods of using and detecting Gm_CSM63717 are also provided. Soybean plants containing the event Gm_CSM63717 exhibit tolerance to PPO inhibitors.

The invention discloses a method for culturing CIK (Cytokine-Induced Killer) cells and application thereof. The method comprises the following steps: recovering cord blood, mixing and centrifuging the recovered cord blood and a buffer solution, re-suspending and precipitating, centrifuging again to obtain cord blood mononuclear cells, inoculating the cord blood mononuclear cells into an activation culture medium, and culturing to obtain the CIK cells, the buffer solution contains L-proline, human recombinant DNA enzyme and angelica polysaccharide. According to the method, the mononuclear cells can be separated from the umbilical cord blood preserved by liquid nitrogen, the utilization rate of the umbilical cord blood which can only be discarded due to insufficient cells is increased, the traditional lymphocyte separation process is optimized, the recovery rate of the mononuclear cells is increased, and the cost is reduced. The method provided by the invention can be used for culturing dozens to ten billions of CIK cells from a small amount of cryopreserved cord blood, and has huge advantages in the field of CIK cell culture and application.

The present invention discloses an Ush1c gene enhancer and its application, belonging to the field of genetic engineering, and the nucleotide sequence of the enhancer is shown in SEQ ID No. 1. The mouse embryo microinjection system is used to confirm that the sequence is an enhancer, which can significantly enhance the transcription activity of the EGFP protein gene in the inner ear. Compared with other sequences of similar size, the enhancer sequence fragment can drive the reporter gene or Ush1c to express significantly in the inner ear, and specifically express it in inner and outer hair cells, and is suitable for recombinant DNA, vectors or adenovirus and other biological materials, and is used to promote the transcription of EGFP gene or other genes in the inner ear.

The present disclosure relates to engineered RNA m6A demethylases with disrupted low complexity regions (LCRs) for expression in plants, as well as recombinant DNA encoding the engineered RNA m6A demethylases, vectors encoding the recombinant DNA, plants including the engineered RNA m6A demethylases, and method of improving the growth of plants using the engineered RNA m6A demethylases, including increased root growth and elevated photosynthesis. The present disclosure further relates to methods of improving growth of a plant, including increased root growth and elevated photosynthesis, by modifying endogenous ALKBH5 homolog genes to include disrupted LCRs, and plants produced by those methods.

A transgenic soybean event, Gm_CSM63717, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gm_CSM63717 are also provided. Recombinant DNA molecules unique to the event Gm_CSM63717, and methods of using and detecting Gm_CSM63717 are also provided. Soybean plants containing the event Gm_CSM63717 exhibit tolerance to PPO inhibitors.

A transgenic cotton event, Gh_CSM63718, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gh_CSM63718 are also provided. Recombinant DNA molecules unique to the event Gh_CSM63718, and methods of using and detecting Gh_CSM63718 are also provided. Cotton plants containing the event Gh_CSM63718 exhibit tolerance to glufosinate, B-triketone HPPD inhibitors, dicamba, glyphosate, PPO inhibitors, and combinations of any thereof.

A series of recombinant DNA constructs and a method is disclosed for use in immunological therapy in general; and in disrupting T cell receptor (TCR), human leukocyte antigens (HLA) class I and NKG2D (Natural-Killer Group 2, member D) ligand expression in particular, with the effect of producing highly compatible autologous universal T cells for further genetically engineering for allogeneic administration. A Universal T (UT) construct is provided and used, comprising a TCR antibody fragment fused to a transmembrane domain (TMD) and ER retention domain of adenovirus early region 3 glycoprotein E3-19k (E3 / 19K) (TCR-E3 / 19K RD). The Universal T (UT) construct can hijack ERAD machinery to arrest TCR and HLA molecules in endoplasmic reticulum (ER) and facilitate their translocation into the cytoplasm for ubiquitination and degradation by proteasomes.

Disclosed herein are methods for rapid transformation, genome editing, and regeneration of dicot plants. The methods disclosed herein involve transforming plant cells and explants with recombinant DNA to achieve genome edits. The methods enhance transformation and regeneration efficiency and reduce the time to produce transgenic plants.

The present invention discloses a Mitf gene enhancer and its application in improving the gene-specific expression of cochlear stria vascular cells, and relates to the field of gene expression and regulation technology. The nucleotide sequence of the enhancer is shown in SEQ ID No. 1. The mouse embryo microinjection system was used to confirm that the sequence is an enhancer, which can significantly enhance the transcription activity of the EGFP protein gene in the cochlear stria vascular cells. Compared with other sequences of similar size, the enhancer sequence fragment can drive the reporter gene or Mitf to be specifically expressed in the cochlear stria vascular cells and the expression level is significantly enhanced. The enhancer is suitable for biological materials such as recombinant DNA, vectors or adenoviruses, and is used to promote the transcription of the EGFP gene or other genes in the cochlear stria vascular cells.

A transgenic rice plant contains a recombinant DNA construct in its genome, the construct comprising a first nucleic acid having a sequence of a first Golden 2-like transcription factor (GLK) gene operably linked to its native promoter and 5' untranslated region (5' untranslated region, 5' UTR), and a second nucleic acid having a sequence of a second GLK gene operably linked to its native promoter and 5' UTR, the second GLK gene being different from the first GLK gene. The first GLK gene and the second GLK gene are both from C4 plants, and compared to untransformed wild-type rice plants, the transgenic rice plant increases shoot biomass by 65-106% and grain yield by 50-95%. A method for producing a transgenic rice plant and a recombinant DNA construct that can be used in the method are also provided.

The invention provides novel recombinant DNA, compositions, and methods for selectively regulating the expression of a transcribable polynucleotide or recombinant protein in pollen of a transgenic plant. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising such recombinant DNA polynucleotides and compositions.

This disclosure provides recombinant DNA mol-encoding fusion proteins that are able to regulate expression of alpha-synuclein. Also provided are various compositions comprising the recombinant DNA molecules, as well as associated methods of use. The recombinant DNA molecules and associated methods are useful for the treatment of subjects having disorders caused by excess expression or intracellular accumulation of alpha-synuclein, including Parkinson's disease.

The present invention belongs to the field of plant molecular biology and relates to the excision of recombinant DNA from the genome of plant somatic cells.

Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and / or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

Recombinant DNA polynucleotides and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants are provided. Also, transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA polynucleotides operably linked to heterologous transcribable DNA polynucleotides are provided. Also provided are methods of the use of the recombinant DNA polynucleotides and constructs.

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

The present disclosure relates to the Field of biotechnology and provides novel recombinant DNA molecules for conferring tolerance to icafolin herbicides. The present disclosure aiso provides herbicide tolérant transgenic plants, seeds, cells, and plant parts comprising the recombinant DNA molécules, and methods of using the same.

The invention relates to biotechnology and provides novel engineered protein for conferring enhanced growth. The invention also provides transgenic plants, seeds, cells and plant parts containing recombinant DNA molecules, as well as methods of using the same.

A recombinant DNA polymerase for sequencing is provided. The recombinant DNA polymerase is a mutant of a 9N° DNA polymerase, the 9N° DNA polymerase mutant can recognize a 3-O-azido-modified reversible terminator and keep high incorporation activity, so that said mutant can be used for second-generation sequencing. The present invention uses a genetically engineered Escherichia coli strain for recombinant expression of the 9N° DNA polymerase mutant, and the expressed mutant enzyme realizes soluble expression in an Escherichia coli system, and therefore has high catalytic activity.

The invention discloses an hph resistance gene knockout box based on gme5 gene editing, a genetically engineered bacterium and application of the hph resistance gene knockout box and the genetically engineered bacterium. The hph resistance gene knockout box comprises a recombinant DNA construction body with the nucleotide sequence shown as SEQ ID NO: 1, the recombinant DNA construction body replaces an endogenous regulatory factor gme5 gene through homologous recombination in a targeted mode, and the nucleotide sequence of the regulatory factor gme5 gene is shown as SEQ ID NO: 2. According to the application, the bumblebee candida albicans are directionally modified through a genetic engineering strategy, an engineering strain for synthesizing the high-yield lactone type sophorolipid is constructed, and the lactonase activity and fatty acid transport capacity are synergistically enhanced, so that the ratio of the lactone type sophorolipid is maximized, and the yield of the lactone type sophorolipid is increased. In the sophorolipid produced by using the genetically engineered bacterium and the fermentation production method disclosed by the invention, the total content of the sophorolipid is 358.77 g / L, and the lactone content accounts for 74% or more of the total sophorolipid, so that the separation and purification difficulty and the production cost are greatly reduced, and an efficient and low-cost solution is provided for industrial fermentation production of the sophorolipid.

The present invention discloses a construction method and application of a mouse conditional inducible Hmox1 gene knockout model, and belongs to the field of biotechnology. The mouse conditional inducible Hmox1 gene knockout model provided by the present invention is constructed by the following steps: constructing a homologous recombination DNA vector; obtaining Cas9mRNA and gRNA for the Hmox1 gene by in vitro transcription; injecting the homologous recombination DNA vector, Cas9mRNA and gRNA into the fertilized egg of the mouse; cultivating to obtain conditional gene knockout mice; identifying the genotype by sequencing and nucleic acid gel electrophoresis; the obtained conditional knockout mice are hybridized with other tissue-specific Cre-expressing mice to obtain corresponding tissue or cell conditional knockout mice, thereby realizing precise research on specific tissues or cell types; and proving that Hmox1 inducible conditional knockout significantly aggravates acute lung injury. The present invention can overcome the difficulties of embryonic lethality of Hmox1 systemic gene knockout mice in the prior art and the inability to construct a mouse model, and assist in the study of Hmox1 in diseases.

The invention discloses application of a tomato gene SlZFP2 as a negative regulation factor in regulation of plant cold resistance, belongs to the technical field of plant genetic engineering, and finds and confirms that the tomato zinc finger protein gene SlZFP2 is a key negative regulation factor of plant cold resistance for the first time. Experiments show that the expression of the SlZFP2 is induced by low temperature, the overexpressed transgenic tomato plant shows more serious cold injury symptoms under low temperature stress, the photosynthetic efficiency is reduced, active oxygen (H2O2 and O2-) is excessively accumulated, and the activity of antioxidant enzymes (SOD, POD and CAT) is weakened; the overexpression of the SlZFP2 can inhibit the biosynthesis and signal transduction of abscisic acid (ABA), and down-regulate the expression of cold response genes SlGRAS4 and SlCBF1; based on the discovery, the invention provides an application of using the SlZFP2 gene or an expression product thereof as a molecular marker for screening tomato materials with different cold resistance, creating a transgenic plant with reduced cold resistance by overexpressing the SlZFP2, and providing a recombinant DNA construct containing an SlZFP2 coding sequence and a host cell.

Provided are transgenic plants expressing MtDef5 antifungal proteins and peptides exhibiting high levels of resistance to susceptible fungi. Such transgenic plants contain a recombinant DNA construct comprising a natural or heterologous signal peptide sequence operably linked to a nucleic acid sequence encoding these molecules. Also provided are methods of producing such plants, methods of protecting plants against susceptible fungal infection and damage, as well as compositions that can be applied to the locus of plants, comprising microorganisms expressing these molecules, or these molecules themselves, as well as pharmaceutical compositions containing these molecules. Human and veterinary therapeutic use of MtDef5 antifungal proteins and peptides to treat susceptible fungal infections are also encompassed by the invention.

The present invention refers to a wheat plant, seed or part of it comprising event IND-ØØ412-7, a commodity product resulting from the seed, a recombinant DNA molecule comprised in event IND-ØØ412-7, DNA primers and probes useful for detecting event IND-ØØ412-7, DNA detection kit, method for producing a wheat plant tolerant to abiotic stresses and method for detecting the presence of DNA belonging to wheat event IND-ØØ412-7 in a sample.

The present disclosure relates to the field of biotechnology and provides novel recombinant DNA molecules for conferring tolerance to icafolin herbicides. The present disclosure also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts comprising the recombinant DNA molecules, and methods of using the same.

The present disclosure relates to novel methods and compositions for conferring glyphosate tolerance to plants. The disclosure also provides glyphosate tolerant plants, seeds, tissues, cells and plant parts comprising the modified EPSP synthase and a recombinant DNA molecule encoding the modified EPSP synthase, as well as methods of producing the glyphosate tolerant plants, seeds, tissues, cells and plant parts and uses thereof.

This invention discloses a targeted adjuvant influenza vaccine composition for atrial fibrillation patients with atrial fibrosis. The vaccine antigen components are selected from the surface antigens of currently prevalent influenza virus strains, such as hemagglutinin (HA) and neuraminidase (NA). High-purity influenza virus antigens are prepared through cell culture or recombinant DNA technology. For example, influenza virus is cultured using Madin-Darby canine kidney (MDCK) cells, and purified through centrifugation, filtration, and chromatography to obtain high-purity HA and NA antigens. After injection into the human body, the influenza virus antigens (HA and NA) are taken up and processed by antigen-presenting cells (such as macrophages and dendritic cells). The antigen-presenting cells present antigen peptides to T lymphocytes and B lymphocytes, activating a specific immune response. B lymphocytes differentiate into plasma cells with the help of T lymphocytes, producing specific antibodies that recognize and bind to the influenza virus, preventing it from infecting host cells and thus preventing influenza virus infection.