87 results about "Recombinant DNA" patented technology

The invention discloses a method for culturing CIK (Cytokine-Induced Killer) cells and application thereof. The method comprises the following steps: recovering cord blood, mixing and centrifuging the recovered cord blood and a buffer solution, re-suspending and precipitating, centrifuging again to obtain cord blood mononuclear cells, inoculating the cord blood mononuclear cells into an activation culture medium, and culturing to obtain the CIK cells, the buffer solution contains L-proline, human recombinant DNA enzyme and angelica polysaccharide. According to the method, the mononuclear cells can be separated from the umbilical cord blood preserved by liquid nitrogen, the utilization rate of the umbilical cord blood which can only be discarded due to insufficient cells is increased, the traditional lymphocyte separation process is optimized, the recovery rate of the mononuclear cells is increased, and the cost is reduced. The method provided by the invention can be used for culturing dozens to ten billions of CIK cells from a small amount of cryopreserved cord blood, and has huge advantages in the field of CIK cell culture and application.

The present disclosure relates to engineered RNA m6A demethylases with disrupted low complexity regions (LCRs) for expression in plants, as well as recombinant DNA encoding the engineered RNA m6A demethylases, vectors encoding the recombinant DNA, plants including the engineered RNA m6A demethylases, and method of improving the growth of plants using the engineered RNA m6A demethylases, including increased root growth and elevated photosynthesis. The present disclosure further relates to methods of improving growth of a plant, including increased root growth and elevated photosynthesis, by modifying endogenous ALKBH5 homolog genes to include disrupted LCRs, and plants produced by those methods.

A transgenic cotton event, Gh_CSM63718, is provided. Transgenic plant cells, plant parts, plants, seeds, progeny plants, and agricultural and commodity products containing event Gh_CSM63718 are also provided. Recombinant DNA molecules unique to the event Gh_CSM63718, and methods of using and detecting Gh_CSM63718 are also provided. Cotton plants containing the event Gh_CSM63718 exhibit tolerance to glufosinate, B-triketone HPPD inhibitors, dicamba, glyphosate, PPO inhibitors, and combinations of any thereof.

Disclosed herein are methods for rapid transformation, genome editing, and regeneration of dicot plants. The methods disclosed herein involve transforming plant cells and explants with recombinant DNA to achieve genome edits. The methods enhance transformation and regeneration efficiency and reduce the time to produce transgenic plants.

The present invention belongs to the field of plant molecular biology and relates to the excision of recombinant DNA from the genome of plant somatic cells.

Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and / or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

The present disclosure relates to the Field of biotechnology and provides novel recombinant DNA molecules for conferring tolerance to icafolin herbicides. The present disclosure aiso provides herbicide tolérant transgenic plants, seeds, cells, and plant parts comprising the recombinant DNA molécules, and methods of using the same.

The invention relates to biotechnology and provides novel engineered protein for conferring enhanced growth. The invention also provides transgenic plants, seeds, cells and plant parts containing recombinant DNA molecules, as well as methods of using the same.

The invention discloses an hph resistance gene knockout box based on gme5 gene editing, a genetically engineered bacterium and application of the hph resistance gene knockout box and the genetically engineered bacterium. The hph resistance gene knockout box comprises a recombinant DNA construction body with the nucleotide sequence shown as SEQ ID NO: 1, the recombinant DNA construction body replaces an endogenous regulatory factor gme5 gene through homologous recombination in a targeted mode, and the nucleotide sequence of the regulatory factor gme5 gene is shown as SEQ ID NO: 2. According to the application, the bumblebee candida albicans are directionally modified through a genetic engineering strategy, an engineering strain for synthesizing the high-yield lactone type sophorolipid is constructed, and the lactonase activity and fatty acid transport capacity are synergistically enhanced, so that the ratio of the lactone type sophorolipid is maximized, and the yield of the lactone type sophorolipid is increased. In the sophorolipid produced by using the genetically engineered bacterium and the fermentation production method disclosed by the invention, the total content of the sophorolipid is 358.77 g / L, and the lactone content accounts for 74% or more of the total sophorolipid, so that the separation and purification difficulty and the production cost are greatly reduced, and an efficient and low-cost solution is provided for industrial fermentation production of the sophorolipid.

The invention discloses application of a tomato gene SlZFP2 as a negative regulation factor in regulation of plant cold resistance, belongs to the technical field of plant genetic engineering, and finds and confirms that the tomato zinc finger protein gene SlZFP2 is a key negative regulation factor of plant cold resistance for the first time. Experiments show that the expression of the SlZFP2 is induced by low temperature, the overexpressed transgenic tomato plant shows more serious cold injury symptoms under low temperature stress, the photosynthetic efficiency is reduced, active oxygen (H2O2 and O2-) is excessively accumulated, and the activity of antioxidant enzymes (SOD, POD and CAT) is weakened; the overexpression of the SlZFP2 can inhibit the biosynthesis and signal transduction of abscisic acid (ABA), and down-regulate the expression of cold response genes SlGRAS4 and SlCBF1; based on the discovery, the invention provides an application of using the SlZFP2 gene or an expression product thereof as a molecular marker for screening tomato materials with different cold resistance, creating a transgenic plant with reduced cold resistance by overexpressing the SlZFP2, and providing a recombinant DNA construct containing an SlZFP2 coding sequence and a host cell.

Provided are transgenic plants expressing MtDef5 antifungal proteins and peptides exhibiting high levels of resistance to susceptible fungi. Such transgenic plants contain a recombinant DNA construct comprising a natural or heterologous signal peptide sequence operably linked to a nucleic acid sequence encoding these molecules. Also provided are methods of producing such plants, methods of protecting plants against susceptible fungal infection and damage, as well as compositions that can be applied to the locus of plants, comprising microorganisms expressing these molecules, or these molecules themselves, as well as pharmaceutical compositions containing these molecules. Human and veterinary therapeutic use of MtDef5 antifungal proteins and peptides to treat susceptible fungal infections are also encompassed by the invention.

The present disclosure relates to the field of biotechnology and provides novel recombinant DNA molecules for conferring tolerance to icafolin herbicides. The present disclosure also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts comprising the recombinant DNA molecules, and methods of using the same.

The present disclosure relates to novel methods and compositions for conferring glyphosate tolerance to plants. The disclosure also provides glyphosate tolerant plants, seeds, tissues, cells and plant parts comprising the modified EPSP synthase and a recombinant DNA molecule encoding the modified EPSP synthase, as well as methods of producing the glyphosate tolerant plants, seeds, tissues, cells and plant parts and uses thereof.

This invention discloses a targeted adjuvant influenza vaccine composition for atrial fibrillation patients with atrial fibrosis. The vaccine antigen components are selected from the surface antigens of currently prevalent influenza virus strains, such as hemagglutinin (HA) and neuraminidase (NA). High-purity influenza virus antigens are prepared through cell culture or recombinant DNA technology. For example, influenza virus is cultured using Madin-Darby canine kidney (MDCK) cells, and purified through centrifugation, filtration, and chromatography to obtain high-purity HA and NA antigens. After injection into the human body, the influenza virus antigens (HA and NA) are taken up and processed by antigen-presenting cells (such as macrophages and dendritic cells). The antigen-presenting cells present antigen peptides to T lymphocytes and B lymphocytes, activating a specific immune response. B lymphocytes differentiate into plasma cells with the help of T lymphocytes, producing specific antibodies that recognize and bind to the influenza virus, preventing it from infecting host cells and thus preventing influenza virus infection.

The invention discloses a Pcdh15 gene enhancer sequence and application thereof, and belongs to the field of gene engineering, and the nucleotide sequence of the enhancer is shown as SEQ ID No.1. A mouse embryo microinjection system is used for verifying that the sequence is an enhancer, and the transcriptional activity of EGFP protein genes in hair cells can be remarkably enhanced in inner ears. Compared with other sequences with similar sizes, the enhancer sequence fragment can drive the expression ability of a reporter gene or a Pcdh15 gene to be obviously enhanced in inner ear hair cells, is specifically expressed in inner and outer hair cells, is suitable for biological materials such as recombinant DNA (Deoxyribonucleic Acid), vectors or adenoviruses and the like, and has a wide application prospect. The method is used for promoting transcription of EGFP genes or other genes in internal and external hair cells of inner ears.

The present invention provides recombinant DNA molecules and constructs useful for modulating gene expression in plants, as well as nucleotide sequences thereof. The present invention also provides transgenic plants, plant cells, plant parts, and seeds comprising a recombinant DNA molecule comprising a DNA molecule operably linked to a heterologous transcribable DNA molecule, as well as methods of use thereof.

The invention provides recombinant DNA polynucleotides and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA polynucleotides operably linked to heterologous transcribable DNA polynucleotides. Also provided are methods of the use of the recombinant DNA polynucleotides and constructs and the transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA polynucleotides and constructs.

The invention belongs to the technical field of in-vitro nucleic acid detection, and discloses a pyrosequencing primer and a kit for detecting CYP2D6-5 genetic typing. And the base sequence of the pyrosequencing primer is as shown in SEQ NO.3. The kit comprises dNTPs, a buffer solution, a recombinant DNA polymerase (rTaq), double distilled water, a specific amplification primer 1, a specific amplification primer 2, a sequencing primer, a blank reference substance and a positive reference substance, the base sequence of the specific amplification primer 1 is as shown in SEQ NO.1; the base sequence of the specific amplification primer 2 is as shown in SEQ NO. 2; the base sequence of the sequencing primer is as shown in SEQ NO.3. When the kit is used for detecting the CYP2D6 * 5 genotyping, the kit has the advantages of accurate qualitative detection, high sensitivity and strong specificity; in addition, the method has the advantages that sample treatment is simple, sequencing steps are simple, the sequencing speed is high, one on-machine reaction is completed in half an hour, detection site frequency analysis is directly given, and the result is visual.

The invention relates to novel methods and compositions for conferring tolerance to glyphosate to plants. The invention also provides glyphosate-tolerant plants, seeds, tissue, cells, and plant parts comprising modified EPSP synthases and recombinant DNA molecules encoding modified EPSP synthases, as well as methods of producing the same and the use thereof.

The present disclosure relates to the use of certain substituted phenyl triazinanes comprising a cyclopropylcarboxylic acid-based side chain, or agrochemically acceptable salts thereof, for controlling or preventing weed growth in plant growth areas of transgenic crop plants that are tolerant to PPO inhibiting herbicides. The disclosure also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same.

The present disclosure provides compositions, methods of use, and methods of creation for a population of transgenic plants derived from plant cells transformed with recombinant DNA for expression of heterologous proteins. In particular, the present disclosure provides compositions comprising indoor ornamental plants suited for the removal of volatile organic compounds such as formaldehyde, benzene, toluene, ethylbenzene and / or xylene from air. Also disclosed are transgenic seeds for growing a transgenic plant having the recombinant DNA in its genome and exhibiting enhanced VOC removal from air. Also disclosed are methods for generating seed and plants based on the transgenic events. Also disclosed are microbes selected for during directed evolution to have enhanced VOC removal from air capabilities. Also disclosed are methods and compositions for generating plant-microbiome pairings for enhanced VOC removal from air.

The present invention relates to recombinant DNA technology, in particular to methods for assembling two or more double stranded (ds) nucleic acid molecules with overlapping terminal sequences. In particular, the present invention relates to the use of a thermolabile DNA polymerase II derived 3′-5′ exonuclease isolated from Moritella viscoa and a thermolabile DNA polymerase I of marine origin in multi DNA assembly processes.

The invention provides recombinant DNA molecules that are unique to maize event MON87429 and transgenic maize plants, maize plant parts, maize seeds, maize cells, and agricultural products containing maize event MON87429 as well as methods of using and detecting maize event MON87429. Transgenic maize plants containing maize event MON87429 exhibit tolerance to inhibitors of acetyl CoA carboxylase (ACCase) in the aryloxyphenoxy propionate (FOP) group, synthetic auxins, inhibitors of glutamine synthetase, and inhibitors of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS).

The invention described herein provides an improved system and method for rAAV production. The system of the invention includes a single recombinant DNA vector that comprises a gene of interest (GO I) to be delivered to a target cell, the Rep and Cap coding sequences, as well as the Helper gene coding sequences for rAAV packaging.

The application belongs to the technical field of molecular biology, and discloses a recombinant DNA polymerase, a preparation method, a kit and application in probe qPCR amplification. The recombinant DNA polymerase provided by the application comprises an amino acid fragment as shown in SEQ ID NO: 1 and / or a variant sequence with more than 90 % homology with the amino acid fragment shown in SEQ ID NO: 1. The recombinant DNA polymerase has excellent anti-inhibition ability and thermal stability, can be well applied to probe qPCR to realize qualitative and quantitative detection of a target sequence in a sample to be detected, especially has good detection effect on complex samples, and has important significance in the application in the fields of clinical diagnosis, food safety detection and the like.

The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.

The present disclosure provides methods for producing heme proteins in transgenic plants, plant tissues, or plant cells, as well as methods for describing the expression of these heme proteins in seeds. The present disclosure also provides transgenic plants that express heme proteins, myoglobin, and hemoglobin by introducing and integrating recombinant DNA constructs into the host genetic material of a plant of interest. Certain combinations of regulatory factors disclosed herein enable high levels of heme protein expression in seeds.

Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and / or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing or nucleic acid amplification. Such properties include enhanced performance with large nucleotide analogs, increased stability, increased readlength, and improved detection of modified bases, and can also include resistance to photodamage, enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased accuracy, altered speed, increased cosolvent resistance, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.