63 results about "Thioesterase" patented technology

The invention provides a protein for synthesizing an octadecene compound and a method for synthesizing the octadecene compound. Relates to the field of enzymology. The amino acid sequence of the protein has more than 95% of consistency with the sequence as shown in SEQ ID No.3. The protein, hexadecanoic acid specific thioesterase, Z11 desaturase, gene silencing suppressor and vegetable fat synthesis related transcription factors are jointly expressed in plants and can be used for synthesizing (Z)-13-octadecenoic acid.

The present disclosure relates to, inter alia, a weed control method. In one example, a method comprises applying to the locus a weed controlling amount of a pesticide composition comprising a FatA acyl-ACP thioesterase-inhibiting herbicide, wherein the crop plants are modified such that they comprise a FatA acyl-ACP thioesterase which provides the crop plant with tolerance against the FatA acyl-ACP thioesterase-inhibiting herbicide. Recombinant polynucleotides and suitable for use in the methods, and edited FatA acyl-ACP thioesterases are also disclosed.

The invention discloses a genetically engineered bacterium, a method for constructing the genetically engineered bacterium and a method for preparing emodin and physcion by using the genetically engineered bacterium. The genetically engineered bacterium carries an exogenous gene; the exogenous gene comprises a gene npgA for coding phosphopantetheinyl transferase and a gene gedC for coding polyketide synthase, further comprises at least one of a gene HyTE and a gene gedB for coding beta-lactamase thioesterase, and further comprises at least one of a decarboxylase gene TpCK and a gene AfDC. According to the invention, a complete synthetic route of the physcion is constructed from the beginning in a heterologous host microorganism for the first time; a novel microbial fermentation mode is used for replacing traditional plant extraction, and novel biological manufacturing of the emodin and the emodin monomethyl ether is developed.

The invention relates to the technical field of genetic engineering, in particular to a gene for biosynthesis of (Z)-13-octadecenoic acid and application of the gene. The invention discloses a gene for biosynthesis of (Z) 13 octadecenoic acid. An amino acid sequence coded by the gene is as shown in SEQ ID NO: 1. The invention finds that the (Z)-13-octadecenoic acid can be synthesized by jointly expressing the protein with the amino acid sequence shown as SEQ ID NO: 1, hexadecanoic acid specific thioesterase and Z11 desaturase in plants. The process of synthesizing the (Z)-13-octadecenoic acid by using plants is green and pollution-free, the purification is simple and convenient, the environmental pollution degree is low, and the extraction purity is high.

The invention relates to the technical field of enzymology, in particular to a gene for biosynthesis of (Z)-9-hexadecenoic acid and application of the gene. The invention discloses a gene for biosynthesis of (Z)-9-hexadecenoic acid. A nucleotide sequence coded by the gene is shown as SEQ ID NO: 1. The gene and hexadecanoic acid specific thioesterase are jointly expressed in plants, so that the biosynthesis yield of (Z)-9-hexadecenoic acid can be increased. The process of synthesizing the (Z)-9-hexadecenoic acid by using plants is green and pollution-free, and the purification is simple and convenient, so that the environmental pollution degree is low, and the extraction purity is high, thereby changing the path depending on chemical synthesis, and solving the defects of complex chemical synthesis, large by-product amount, environmental pollution, high cost, low final product purity and the like.

This invention relates to the biotechnological production of ω-functionalized carboxylic acids and their esters. Microbial cells are provided for the production of at least one ω-functionalized carboxylic acid ester from at least one alkane, wherein the cells are genetically modified relative to wild-type cells to increase the expression of: (i) an enzyme E1 capable of converting the alkane to the corresponding 1-alkanol; (ii) an enzyme E2 capable of converting the 1-alkanol of (i) to the corresponding 1-alkanal; (iii) an enzyme E3 capable of converting the 1-alkanal of (ii) to the corresponding alkanoic acid; (iv) an enzyme E4 capable of converting the alkanoic acid of (iii) to the corresponding alkanoic acid ester; and (iv) an enzyme E5 capable of converting the alkanoic acid ester of (iv) to the corresponding ω-hydroxy-alkanoic acid ester, and wherein the cells do not contain an increase relative to wild-type cells of the expression of the following enzymes selected from the group consisting of: 20 -E 24 Genetic modification of expression of at least one of them: -E 20 Acyl-ACP thioesterase, -E 21 Acyl-CoA thioesterase, -E 22 Acyl-CoA:ACP transacylase, -E 23 Polyketide synthase, and -E 24 Hexanoic acid synthase.

The present invention is directed to a transgenic soybean plant having increased oleic acid content comprising a polynucleotide comprising a fatty acid thioesterase (FAT) related promoter that functions in the soybean plant operably linked to a polynucleotide encoding a polypeptide having FAT activity. The invention is further directed to a method of increasing oleic acid content of a soybean plant comprising transforming a soybean plant with a polynucleotide comprising a fatty acid thioesterase (FAT) related promoter that functions in the soybean plant operably linked to a polynucleotide encoding a polypeptide having FAT activity.

The disclosure relates to engineered plant acyl-ACP thioesterases having improved activity for the production of medium-chain fatty acid derivatives including e.g., eight carbon and ten carbon fatty acids and fatty acid derivatives. The disclosure further relates to recombinant host cells comprising the engineered plant acyl-ACP thioesterases having improved activity for the production of medium-chain fatty acid derivatives. The disclosure also relates to methods of decreasing toxicity and improving production of medium-chain fatty acids and derivatives.

The invention provides a method for improving the molecular weight of polyhydroxyalkanoates (polyhydroxyalkanoates, called PHA for short) synthesized by microorganisms, and relates to a method for improving the molecular weight of the polyhydroxyalkanoates (polyhydroxyalkanoates, called PHA for short). By knocking out or knocking down endogenous (intracellular) carboxylesterase, lipase, esterase, thioesterase and PHA degrading enzyme genes of bacteria for synthesizing PHA, the molecular weight of various synthesized PHA is obviously improved. The method has universality for improving the molecular weight of various PHAs synthesized by different species of bacteria, the mechanical property of the PHA can be improved by improving the molecular weight of the PHA, and new performance is provided for expanding the application of the PHA.

The present invention relates to a monomer self-assembled and polymerized by thioesterase and reactive oxygen species in cancer cells and a use thereof. According to the present invention, the monomer represented by chemical formula 1 is selectively accumulated in mitochondria and undergoes the deprotection of the acetyl protecting group from the thioacetyl group by thioesterase within cancerous cells to expose a thiol group which is then activated by reactive oxygen species (ROS) within cancerous cells to form disulfide bonds, leading to self-assembly polymerization into a macromolecular polymer that selectively disrupts cancerous mitochondria. In normal cells, deprotection of the acetyl group does not occur, so the monomer remains as it is, exerting no effect.

The present invention provides a microorganism capable of producing at least one C6-C26 free fatty acid or a mixture thereof from methanol, said microorganism comprising at least one gene enabling the expression of a thioesterase (TE) and at least one gene enabling the expression of at least one Fatty Alcohol Reductase (FAR). The present invention further provides methods for the manufacture of at least one C6-C26 free fatty acids or mixtures thereof and C6-C26 free fatty alcohols or mixtures thereof.

The invention belongs to the technical field of biological genetic genes and aquatic organisms, and discloses an SNP (Single Nucleotide Polymorphism) marker related to rapid growth traits of Mastacembelus armatus and application of the SNP marker, the SNP marker is shown as SEQ ID NO: 1, and the base site at the 1305th site in a palmitoyl protein thioesterase 1 gene sequence is C or G. The invention also provides a primer pair and a kit. According to the invention, growth traits of a mastacembelus armatus mixed family group are analyzed through whole genome association analysis, an SNP allele related to rapid growth of mastacembelus armatus is determined, and a method for rapidly, accurately and effectively detecting rapid-growing individuals of mastacembelus armatus by using the SNP marker is established. The method can be used for identifying the mastacembelus armatus individuals with excellent growth traits in the early stage so as to shorten the breeding period of the mastacembelus armatus, and is of great significance to industrial screening and cultivation of mastacembelus armatus varieties which grow quickly.

This invention provides fatty diols and recombinant microorganisms for producing them. [Solution] A recombinant microorganism for producing 1,3-lipid diols when grown in a fermentation broth containing a simple carbon source, wherein the recombinant microorganism is engineered to express a nucleic acid sequence encoding a polypeptide that includes (a) thioesterase (EC3.1.2.-, EC3.1.1.5, or EC3.1.2.14) activity and (b) carboxylic acid reductase (EC6.2.1.3 or EC1.2.1.42) activity.

A method of biocatalytic cyclization for the preparation of cyclic peptides or salts thereof using Ulm16, a penicillin-binding protein (PBP)-type class of thioesterase (PBP-TE) enzyme.

Recombinant cells and methods for producing methyl ketones, such as medium-chain methyl ketones. The recombinant cells include recombinant acyl-ACP thioesterase genes, recombinant β-ketoacyl-CoA thioesterase genes, and recombinant acyl-CoA synthetase genes, in addition to other modifications. The methods include culturing the recombinant cells to produce the methyl ketones and isolating the produced methyl ketones.

The present application relates to a kind of containing fatty acyl-coa dehydrogenase engineering bacteria and its application method, belong to microbial fermentation technical field.The engineering bacteria containing fatty acyl-coa dehydrogenase gene in the present application include fatty acyl-coa dehydrogenase gene and fatty acyl-coa thioesterase gene, the fatty acyl-coa dehydrogenase gene is derived from Candida tropicalis or Yarrowia lipolytica, its nucleotide sequence is SEQ ID NO.1 or SEQ ID NO.2;The nucleotide sequence of the fatty acyl-coa thioesterase gene is SEQ ID NO.3.Fatty acyl-coa dehydrogenase from Candida tropicalis and Yarrowia lipolytica, combined with existing fatty acyl-coa thioesterase, jointly convert 10-hydroxy decanoic acid, significantly improve the conversion rate of 10-hydroxy-2-decenoic acid, so that the industrialization production of 10-hydroxy-2-decenoic acid becomes possible.

The present invention provides a microorganism capable of producing at least one C6-C26 free fatty acid or a mixture thereof from methanol, said microorganism comprising at least one gene enabling the expression of a thioesterase (TE) and at least one gene enabling the expression of at least one Fatty Alcohol Reductase (FAR). The present invention further provides methods for the manufacture of at least one C6-C26 free fatty acids or mixtures thereof and C6-C26 free fatty alcohols or mixtures thereof.

This disclosure describes transgenic plants engineered for enhanced amounts of fatty acids and triacylglycerols (TAGs) in vegetative tissues. This document describes novel approaches to develop biomass crops, such as Sorghum, for production of vegetative sources of vegetable oils in leaves and stems as alternative renewable diesel and sustainable aviation fuel (SAF) feedstocks. A new approach is described for engineering high vegetative oil production that involves expression of variant medium-chain fatty acid acyl-acyl carrier protein (AGP) thioesterases to drive vegetable oil production. This approach has been shown to be effective with two different acyl-ACP thioesterases, and the high oil production is maintained over multiple genetic generations in greenhouse and field cultivation systems.

This invention discloses a tomato thioesterase gene. SlFATB2 and its application in regulating tomato growth, development, and disease resistance, the aforementioned SlFATB2 The gene has the nucleotide sequence shown in SEQ ID No. 1. Its CDS sequence was cloned using bioinformatics combined with molecular biology techniques, and a strong promoter was constructed. SlFATB2 Overexpression vectors were used to obtain transgenic tomato plants overexpressing the vectors. The CRISPR / Cas9 knockout system was used to obtain... SlFATB2 Gene knockout tomato transgenic lines. Experiments have confirmed that knockout... SlFATB2 This leads to pollen shrinkage, fruit and seed shrinkage, and susceptibility to gray mold; overexpression SlFATB2 It can enhance resistance to gray mold without affecting normal growth. This invention clarifies that... SlFATB2 The gene function can be precisely improved in tomato agronomic traits and disease resistance through gene editing or overexpression, and its application prospects are broad.

The invention discloses an Escherichia coli strain capable of efficiently secreting and expressing alpha-lipoic acid and application of the Escherichia coli strain, and belongs to the technical field of biology. Escherichia coli is used as a chassis strain for synthesizing lipoic acid by a microbiological method, and an efficient production strain is constructed by optimizing a metabolic pathway; the related modification comprises the following steps: overexpressing a lipoic amidase coding gene eflpA, a truncated thioesterase mutant 'TesA coding gene, a lipoyl synthase coding gene lipA, an iron-sulfur cluster regeneration gene cluster coding gene iscSUA, an S-adenosylmethionine synthetase coding gene sam2 and a 5'-methyl adenosine thioate / S-adenosylhomocysteine nucleoside enzyme coding gene mtn, and carrying out gene coding. A fatty acid degrading enzyme coding gene fadE, a thioesterase coding gene tesA, a pyruvate formate lyase gene cluster coding gene pflAB and a glucokinase coding gene glk are knocked out. According to the invention, the Escherichia coli realizes efficient de novo synthesis and secretory expression of lipoic acid, and finally the extracellular yield in the shake flask fermentation process reaches 16.96 mg / L.

The invention discloses a TE enzyme mutant with improved activity and application thereof, and belongs to the technical field of biology. Schrodinger molecular docking is combined with fixed-point saturation mutation to establish a mutant library, Rosetta is used for screening the mutant library, searching a target point and carrying out single-point mutation and combined mutation, the activity of thioesterase is improved, the synthesis efficiency of Surfactin is further improved, and a production strain with better fermentation performance in industrial production is obtained. Through verification, mutations comprise A113W, A113G, K122H, K192D and a combination of the A113W, the A113G, the K122H and the K192D, fermentation verification shows that the yield of the Surfactin is increased by 41.2% to the maximum compared with that of a wild type and reaches 9.6 g / L, thioesterase with higher activity and a production strain with better fermentation performance in industrial production are obtained, and powerful support is provided for efficient production of the Surfactin.

This invention relates to a protein and method for synthesizing cis-11-tetradecenoic acid. The invention discovers that cis-11-tetradecenoic acid can be synthesized by co-expressing a protein with the amino acid sequence shown in SEQ ID No. 2 and a fourteen-carbon fatty acid-specific thioesterase (TE14) in plants. The process of synthesizing cis-11-tetradecenoic acid from plants is green and pollution-free. Due to its simple and convenient purification, it has a low degree of environmental pollution and high purity. This changes the reliance on chemical synthesis and solves the drawbacks of chemical synthesis, such as complexity, large amounts of byproducts, environmental pollution, high cost, and low purity of the final product.

The invention relates to the technical field of bioengineering, in particular to a method for improving amino acid production of microorganisms. The invention finds that the thioesterase family protein with the amino acid sequence shown as SEQ ID NO.4 has obvious correlation with the yield of lysine, and finds that the thioesterase family protein mutant containing a specific mutation site can obviously promote microbial synthesis and accumulate lysine. The thioesterase family protein mutant provided by the invention is introduced into a microorganism, so that the lysine yield of the microorganism is remarkably improved, and the thioesterase family protein mutant has important significance in improving the fermentation production performance of the microorganism and reducing the production cost of lysine.

This application relates to the field of genetically engineered bacteria technology, specifically disclosing a genetically engineered *E. coli* strain for producing emodin, its construction method, and its applications. This application uses *E. coli* BAP1 as the substrate strain. This strain carries its own sfp in its genome, which can be used to activate polyketide synthase. Furthermore, *E. coli* itself grows rapidly, has a vigorous metabolism, and the culture medium is low-cost, facilitating high-density fermentation. Through gene editing, the uptake and utilization of glucose are improved, the flux of acetic acid and lactic acid diverted from pyruvate is reduced, and the ATP supply is enhanced to increase the flux of acetyl-CoA and malonyl-CoA within the bacteria. This provides sufficient substrate for the synthesis of emodin by polyketide synthase, thioesterase, and decarboxylase, thereby increasing the yield of emodin. The emodin yield achieved by this application through shake-flask fermentation reaches 576.4 mg / L, which is currently the highest level in the *E. coli* system.

A method for improving the synthetic ability of *Yersinia lipolytica* to synthesize amino acid derivatives. This invention discloses an engineered strain for the biological synthesis of the amino acid derivative β-hydroxy-β-methylbutyric acid (β-methylbutyric acid) and its construction method. Using *Yersinia lipolytica* as the substrate cell, the HMGCL and OCT genes involved in branched-chain amino acid metabolism in its genome are knocked out to obtain a gene knockout strain. Decarboxylase genes, hydratase genes, dehydratase genes, and thioesterase genes are integrated into the genome of the gene knockout strain to obtain an engineered strain capable of de novo synthesis of β-hydroxy-β-methylbutyric acid. Further integration of the CcGCTB-CcGCTA fusion gene expression module enhances the expression level of key enzyme genes, thereby increasing the yield of β-hydroxy-β-methylbutyric acid in the strain. The engineered strain constructed in this invention can synthesize the target product β-hydroxy-β-methylbutyric acid using glucose as a carbon source through fermentation, with a maximum yield of up to 30 g / L.

The invention provides a protein and a method for synthesizing an insect sex pheromone precursor compound. The invention finds that 10-hexadecenoic acid can be synthesized by jointly expressing the protein with the amino acid sequence shown as SEQ ID No. 2 and hexadecanoic acid specific thioesterase in plants. The process of synthesizing the 10-hexadecenoic acid by using plants is green and pollution-free, and the purification is simple and convenient, so that the degree of environmental pollution is low, and the extraction purity is high, thereby changing the path depending on chemical synthesis, and solving the defects of complex chemical synthesis, large by-product amount, environmental pollution, high cost, low final product purity and the like.

The invention relates to the technical field of gene engineering, in particular to a method for producing an insect sex pheromone precursor by utilizing rape and application. The invention provides a method for biosynthesizing an insect sex pheromone precursor (Z)-11-hexadecenoic acid in oilseed rape. The method comprises the step of co-expressing a gene for coding hexadecanoic fatty acid specific thioesterase and at least one gene for coding Z11 desaturase in the oilseed rape. The technical breakthrough of efficiently synthesizing the insect sex pheromone precursor in the rape seeds is realized. According to the invention, the insect sex pheromone key precursor (Z)-11-hexadecenoic acid is successfully heterologously synthesized in the rape seeds for the first time, and the content of the Z)-11-hexadecenoic acid accounts for about 24.5% of the total fatty acid of the seeds and is obviously higher than about 20% reported in camelina sativa.

Disclosed is an inhibitor of the acyl-protein thioesterase APT1, ML-348, of formula A:for use in the treatment and / or prevention of a disease or disorder in a subject in need thereof. In particular the inhibitor is for use in the treatment and / or prevention of a neurodegenerative disease or disorder, particularly Huntington's disease. Also disclosed is a pharmaceutical composition including at least the APT1 inhibitor and at least one pharmaceutically acceptable excipient. The inhibitor or composition is intended for use as a medicament. Furthermore, the inhibitor or composition is intended for use in the treatment and / or prevention of a disease or disorder related to impaired intracellular trafficking between the endoplasmic reticulum and the Golgi apparatus and between the latter and the plasma membrane.

The invention discloses application of a Smad1 palmitoylation regulator in preparation of a medicine for enhancing cis-platinum sensitivity of high-grade serous ovarian cancer. Research finds that Smad1 of high-grade serous ovarian cancer has a palmitoylation state, and the effect of enhancing cis-platinum sensitivity of the high-grade serous ovarian cancer by stimulating palmitoylation of Smad1 is achieved. In addition, specific palmitoyl S-acyltransferase subjected to palmitoylation of the Smad1 and acyl protein thioesterase 1 subjected to palmitoylation removal are screened, and a molecular mechanism for regulating cis-platinum drug resistance of the high-grade serous ovarian cancer through palmitoylation modification of the Smad1 is defined. The Smad1 palmitoylation regulator disclosed by the invention can be used for effectively improving the palmitoylation level of Smad1, so that the cis-platinum sensitivity of high-grade serous ovarian cancer is enhanced.

The present invention provides a microorganism capable of producing at least one C6-C26 free fatty alcohol or a mixture thereof from methanol, said microorganism comprising at least one gene enabling the expression of a thioesterase (TE), at least one gene enabling the expression of at least one carboxylic acid reductase (CAR) and at least one gene enabling the expression of an aldehyde reductase (ALDR). The present invention further provides methods for the manufacture of C6-C26 free fatty alcohols or mixtures thereof, as well as a free fatty alcohol composition obtained from these methods.