36 results about "Thioesterase" patented technology

The invention relates to the technical field of genetic engineering, in particular to a gene for biosynthesis of (Z)-13-octadecenoic acid and application of the gene. The invention discloses a gene for biosynthesis of (Z) 13 octadecenoic acid. An amino acid sequence coded by the gene is as shown in SEQ ID NO: 1. The invention finds that the (Z)-13-octadecenoic acid can be synthesized by jointly expressing the protein with the amino acid sequence shown as SEQ ID NO: 1, hexadecanoic acid specific thioesterase and Z11 desaturase in plants. The process of synthesizing the (Z)-13-octadecenoic acid by using plants is green and pollution-free, the purification is simple and convenient, the environmental pollution degree is low, and the extraction purity is high.

The invention relates to the technical field of enzymology, in particular to a gene for biosynthesis of (Z)-9-hexadecenoic acid and application of the gene. The invention discloses a gene for biosynthesis of (Z)-9-hexadecenoic acid. A nucleotide sequence coded by the gene is shown as SEQ ID NO: 1. The gene and hexadecanoic acid specific thioesterase are jointly expressed in plants, so that the biosynthesis yield of (Z)-9-hexadecenoic acid can be increased. The process of synthesizing the (Z)-9-hexadecenoic acid by using plants is green and pollution-free, and the purification is simple and convenient, so that the environmental pollution degree is low, and the extraction purity is high, thereby changing the path depending on chemical synthesis, and solving the defects of complex chemical synthesis, large by-product amount, environmental pollution, high cost, low final product purity and the like.

This invention relates to the biotechnological production of ω-functionalized carboxylic acids and their esters. Microbial cells are provided for the production of at least one ω-functionalized carboxylic acid ester from at least one alkane, wherein the cells are genetically modified relative to wild-type cells to increase the expression of: (i) an enzyme E1 capable of converting the alkane to the corresponding 1-alkanol; (ii) an enzyme E2 capable of converting the 1-alkanol of (i) to the corresponding 1-alkanal; (iii) an enzyme E3 capable of converting the 1-alkanal of (ii) to the corresponding alkanoic acid; (iv) an enzyme E4 capable of converting the alkanoic acid of (iii) to the corresponding alkanoic acid ester; and (iv) an enzyme E5 capable of converting the alkanoic acid ester of (iv) to the corresponding ω-hydroxy-alkanoic acid ester, and wherein the cells do not contain an increase relative to wild-type cells of the expression of the following enzymes selected from the group consisting of: 20 -E 24 Genetic modification of expression of at least one of them: -E 20 Acyl-ACP thioesterase, -E 21 Acyl-CoA thioesterase, -E 22 Acyl-CoA:ACP transacylase, -E 23 Polyketide synthase, and -E 24 Hexanoic acid synthase.

The invention provides a method for improving the molecular weight of polyhydroxyalkanoates (polyhydroxyalkanoates, called PHA for short) synthesized by microorganisms, and relates to a method for improving the molecular weight of the polyhydroxyalkanoates (polyhydroxyalkanoates, called PHA for short). By knocking out or knocking down endogenous (intracellular) carboxylesterase, lipase, esterase, thioesterase and PHA degrading enzyme genes of bacteria for synthesizing PHA, the molecular weight of various synthesized PHA is obviously improved. The method has universality for improving the molecular weight of various PHAs synthesized by different species of bacteria, the mechanical property of the PHA can be improved by improving the molecular weight of the PHA, and new performance is provided for expanding the application of the PHA.

The present invention relates to a monomer self-assembled and polymerized by thioesterase and reactive oxygen species in cancer cells and a use thereof. According to the present invention, the monomer represented by chemical formula 1 is selectively accumulated in mitochondria and undergoes the deprotection of the acetyl protecting group from the thioacetyl group by thioesterase within cancerous cells to expose a thiol group which is then activated by reactive oxygen species (ROS) within cancerous cells to form disulfide bonds, leading to self-assembly polymerization into a macromolecular polymer that selectively disrupts cancerous mitochondria. In normal cells, deprotection of the acetyl group does not occur, so the monomer remains as it is, exerting no effect.

The present invention provides a microorganism capable of producing at least one C6-C26 free fatty acid or a mixture thereof from methanol, said microorganism comprising at least one gene enabling the expression of a thioesterase (TE) and at least one gene enabling the expression of at least one Fatty Alcohol Reductase (FAR). The present invention further provides methods for the manufacture of at least one C6-C26 free fatty acids or mixtures thereof and C6-C26 free fatty alcohols or mixtures thereof.

This invention provides fatty diols and recombinant microorganisms for producing them. [Solution] A recombinant microorganism for producing 1,3-lipid diols when grown in a fermentation broth containing a simple carbon source, wherein the recombinant microorganism is engineered to express a nucleic acid sequence encoding a polypeptide that includes (a) thioesterase (EC3.1.2.-, EC3.1.1.5, or EC3.1.2.14) activity and (b) carboxylic acid reductase (EC6.2.1.3 or EC1.2.1.42) activity.

Recombinant cells and methods for producing methyl ketones, such as medium-chain methyl ketones. The recombinant cells include recombinant acyl-ACP thioesterase genes, recombinant β-ketoacyl-CoA thioesterase genes, and recombinant acyl-CoA synthetase genes, in addition to other modifications. The methods include culturing the recombinant cells to produce the methyl ketones and isolating the produced methyl ketones.

The present application relates to a kind of containing fatty acyl-coa dehydrogenase engineering bacteria and its application method, belong to microbial fermentation technical field.The engineering bacteria containing fatty acyl-coa dehydrogenase gene in the present application include fatty acyl-coa dehydrogenase gene and fatty acyl-coa thioesterase gene, the fatty acyl-coa dehydrogenase gene is derived from Candida tropicalis or Yarrowia lipolytica, its nucleotide sequence is SEQ ID NO.1 or SEQ ID NO.2;The nucleotide sequence of the fatty acyl-coa thioesterase gene is SEQ ID NO.3.Fatty acyl-coa dehydrogenase from Candida tropicalis and Yarrowia lipolytica, combined with existing fatty acyl-coa thioesterase, jointly convert 10-hydroxy decanoic acid, significantly improve the conversion rate of 10-hydroxy-2-decenoic acid, so that the industrialization production of 10-hydroxy-2-decenoic acid becomes possible.

The present invention provides a microorganism capable of producing at least one C6-C26 free fatty acid or a mixture thereof from methanol, said microorganism comprising at least one gene enabling the expression of a thioesterase (TE) and at least one gene enabling the expression of at least one Fatty Alcohol Reductase (FAR). The present invention further provides methods for the manufacture of at least one C6-C26 free fatty acids or mixtures thereof and C6-C26 free fatty alcohols or mixtures thereof.

This disclosure describes transgenic plants engineered for enhanced amounts of fatty acids and triacylglycerols (TAGs) in vegetative tissues. This document describes novel approaches to develop biomass crops, such as Sorghum, for production of vegetative sources of vegetable oils in leaves and stems as alternative renewable diesel and sustainable aviation fuel (SAF) feedstocks. A new approach is described for engineering high vegetative oil production that involves expression of variant medium-chain fatty acid acyl-acyl carrier protein (AGP) thioesterases to drive vegetable oil production. This approach has been shown to be effective with two different acyl-ACP thioesterases, and the high oil production is maintained over multiple genetic generations in greenhouse and field cultivation systems.

This invention discloses a tomato thioesterase gene. SlFATB2 and its application in regulating tomato growth, development, and disease resistance, the aforementioned SlFATB2 The gene has the nucleotide sequence shown in SEQ ID No. 1. Its CDS sequence was cloned using bioinformatics combined with molecular biology techniques, and a strong promoter was constructed. SlFATB2 Overexpression vectors were used to obtain transgenic tomato plants overexpressing the vectors. The CRISPR / Cas9 knockout system was used to obtain... SlFATB2 Gene knockout tomato transgenic lines. Experiments have confirmed that knockout... SlFATB2 This leads to pollen shrinkage, fruit and seed shrinkage, and susceptibility to gray mold; overexpression SlFATB2 It can enhance resistance to gray mold without affecting normal growth. This invention clarifies that... SlFATB2 The gene function can be precisely improved in tomato agronomic traits and disease resistance through gene editing or overexpression, and its application prospects are broad.

The invention discloses an Escherichia coli strain capable of efficiently secreting and expressing alpha-lipoic acid and application of the Escherichia coli strain, and belongs to the technical field of biology. Escherichia coli is used as a chassis strain for synthesizing lipoic acid by a microbiological method, and an efficient production strain is constructed by optimizing a metabolic pathway; the related modification comprises the following steps: overexpressing a lipoic amidase coding gene eflpA, a truncated thioesterase mutant 'TesA coding gene, a lipoyl synthase coding gene lipA, an iron-sulfur cluster regeneration gene cluster coding gene iscSUA, an S-adenosylmethionine synthetase coding gene sam2 and a 5'-methyl adenosine thioate / S-adenosylhomocysteine nucleoside enzyme coding gene mtn, and carrying out gene coding. A fatty acid degrading enzyme coding gene fadE, a thioesterase coding gene tesA, a pyruvate formate lyase gene cluster coding gene pflAB and a glucokinase coding gene glk are knocked out. According to the invention, the Escherichia coli realizes efficient de novo synthesis and secretory expression of lipoic acid, and finally the extracellular yield in the shake flask fermentation process reaches 16.96 mg / L.

The invention discloses a TE enzyme mutant with improved activity and application thereof, and belongs to the technical field of biology. Schrodinger molecular docking is combined with fixed-point saturation mutation to establish a mutant library, Rosetta is used for screening the mutant library, searching a target point and carrying out single-point mutation and combined mutation, the activity of thioesterase is improved, the synthesis efficiency of Surfactin is further improved, and a production strain with better fermentation performance in industrial production is obtained. Through verification, mutations comprise A113W, A113G, K122H, K192D and a combination of the A113W, the A113G, the K122H and the K192D, fermentation verification shows that the yield of the Surfactin is increased by 41.2% to the maximum compared with that of a wild type and reaches 9.6 g / L, thioesterase with higher activity and a production strain with better fermentation performance in industrial production are obtained, and powerful support is provided for efficient production of the Surfactin.

This invention relates to a protein and method for synthesizing cis-11-tetradecenoic acid. The invention discovers that cis-11-tetradecenoic acid can be synthesized by co-expressing a protein with the amino acid sequence shown in SEQ ID No. 2 and a fourteen-carbon fatty acid-specific thioesterase (TE14) in plants. The process of synthesizing cis-11-tetradecenoic acid from plants is green and pollution-free. Due to its simple and convenient purification, it has a low degree of environmental pollution and high purity. This changes the reliance on chemical synthesis and solves the drawbacks of chemical synthesis, such as complexity, large amounts of byproducts, environmental pollution, high cost, and low purity of the final product.

This application relates to the field of genetically engineered bacteria technology, specifically disclosing a genetically engineered *E. coli* strain for producing emodin, its construction method, and its applications. This application uses *E. coli* BAP1 as the substrate strain. This strain carries its own sfp in its genome, which can be used to activate polyketide synthase. Furthermore, *E. coli* itself grows rapidly, has a vigorous metabolism, and the culture medium is low-cost, facilitating high-density fermentation. Through gene editing, the uptake and utilization of glucose are improved, the flux of acetic acid and lactic acid diverted from pyruvate is reduced, and the ATP supply is enhanced to increase the flux of acetyl-CoA and malonyl-CoA within the bacteria. This provides sufficient substrate for the synthesis of emodin by polyketide synthase, thioesterase, and decarboxylase, thereby increasing the yield of emodin. The emodin yield achieved by this application through shake-flask fermentation reaches 576.4 mg / L, which is currently the highest level in the *E. coli* system.

A method for improving the synthetic ability of *Yersinia lipolytica* to synthesize amino acid derivatives. This invention discloses an engineered strain for the biological synthesis of the amino acid derivative β-hydroxy-β-methylbutyric acid (β-methylbutyric acid) and its construction method. Using *Yersinia lipolytica* as the substrate cell, the HMGCL and OCT genes involved in branched-chain amino acid metabolism in its genome are knocked out to obtain a gene knockout strain. Decarboxylase genes, hydratase genes, dehydratase genes, and thioesterase genes are integrated into the genome of the gene knockout strain to obtain an engineered strain capable of de novo synthesis of β-hydroxy-β-methylbutyric acid. Further integration of the CcGCTB-CcGCTA fusion gene expression module enhances the expression level of key enzyme genes, thereby increasing the yield of β-hydroxy-β-methylbutyric acid in the strain. The engineered strain constructed in this invention can synthesize the target product β-hydroxy-β-methylbutyric acid using glucose as a carbon source through fermentation, with a maximum yield of up to 30 g / L.

The invention provides a protein and a method for synthesizing an insect sex pheromone precursor compound. The invention finds that 10-hexadecenoic acid can be synthesized by jointly expressing the protein with the amino acid sequence shown as SEQ ID No. 2 and hexadecanoic acid specific thioesterase in plants. The process of synthesizing the 10-hexadecenoic acid by using plants is green and pollution-free, and the purification is simple and convenient, so that the degree of environmental pollution is low, and the extraction purity is high, thereby changing the path depending on chemical synthesis, and solving the defects of complex chemical synthesis, large by-product amount, environmental pollution, high cost, low final product purity and the like.

The invention relates to the technical field of gene engineering, in particular to a method for producing an insect sex pheromone precursor by utilizing rape and application. The invention provides a method for biosynthesizing an insect sex pheromone precursor (Z)-11-hexadecenoic acid in oilseed rape. The method comprises the step of co-expressing a gene for coding hexadecanoic fatty acid specific thioesterase and at least one gene for coding Z11 desaturase in the oilseed rape. The technical breakthrough of efficiently synthesizing the insect sex pheromone precursor in the rape seeds is realized. According to the invention, the insect sex pheromone key precursor (Z)-11-hexadecenoic acid is successfully heterologously synthesized in the rape seeds for the first time, and the content of the Z)-11-hexadecenoic acid accounts for about 24.5% of the total fatty acid of the seeds and is obviously higher than about 20% reported in camelina sativa.

Disclosed is an inhibitor of the acyl-protein thioesterase APT1, ML-348, of formula A:for use in the treatment and / or prevention of a disease or disorder in a subject in need thereof. In particular the inhibitor is for use in the treatment and / or prevention of a neurodegenerative disease or disorder, particularly Huntington's disease. Also disclosed is a pharmaceutical composition including at least the APT1 inhibitor and at least one pharmaceutically acceptable excipient. The inhibitor or composition is intended for use as a medicament. Furthermore, the inhibitor or composition is intended for use in the treatment and / or prevention of a disease or disorder related to impaired intracellular trafficking between the endoplasmic reticulum and the Golgi apparatus and between the latter and the plasma membrane.

The invention discloses application of a Smad1 palmitoylation regulator in preparation of a medicine for enhancing cis-platinum sensitivity of high-grade serous ovarian cancer. Research finds that Smad1 of high-grade serous ovarian cancer has a palmitoylation state, and the effect of enhancing cis-platinum sensitivity of the high-grade serous ovarian cancer by stimulating palmitoylation of Smad1 is achieved. In addition, specific palmitoyl S-acyltransferase subjected to palmitoylation of the Smad1 and acyl protein thioesterase 1 subjected to palmitoylation removal are screened, and a molecular mechanism for regulating cis-platinum drug resistance of the high-grade serous ovarian cancer through palmitoylation modification of the Smad1 is defined. The Smad1 palmitoylation regulator disclosed by the invention can be used for effectively improving the palmitoylation level of Smad1, so that the cis-platinum sensitivity of high-grade serous ovarian cancer is enhanced.

The present invention provides a microorganism capable of producing at least one C6-C26 free fatty alcohol or a mixture thereof from methanol, said microorganism comprising at least one gene enabling the expression of a thioesterase (TE), at least one gene enabling the expression of at least one carboxylic acid reductase (CAR) and at least one gene enabling the expression of an aldehyde reductase (ALDR). The present invention further provides methods for the manufacture of C6-C26 free fatty alcohols or mixtures thereof, as well as a free fatty alcohol composition obtained from these methods.

The present application relates to a kind of genetically engineered bacteria and its application in preparation 10-hydroxy-2-decenoic acid, belong to biological fermentation technical field.The genetically engineered bacteria of the present application includes fatty acyl-coa oxidase gene, fatty acyl-coa thioesterase gene and CYP153AM228L-CPR BM3 Fusion enzyme gene, the fatty acyl-coa oxidase gene is derived from Candida tropicalis, its nucleotide sequence is SEQ ID NO.3.The key enzyme of biosynthesis 10-hydroxy-2-decenoic acid is supplemented, integrated in the present application, fatty acid hydroxylase, fatty acyl-coa oxidase, fatty acyl-coa thioesterase is jointly transferred into host bacteria, so that each enzyme system in engineering bacteria synergistic effect, to achieve the purpose of decanoic acid as raw material one-step synthesis 10-hydroxy-2-decenoic acid.

When employed, a recombinant expression vector carrying a daptomycin production gene including a foreign fatty acyl AMP ligase (FAAL) gene, a thioesterase (TE) gene, and a fatty acid synthase (FAS) gene according to the present invention can enhance specificity for decanoic acid, and produce daptomycin without supplementation of lipopeptides, such as decanoic acid, from the outside of the strain. In addition, no by-products are generated during the production process, thereby enabling production of daptomycin with high purity and high yield.

This invention relates to the field of genetic engineering technology, and more particularly to a gene for the biosynthesis of (Z)-13-octadecenoic acid and its application. A gene for the biosynthesis of (Z)-13-octadecenoic acid is provided, the amino acid sequence of which is shown in SEQ ID NO: 1. This invention discovers that expressing a protein with the amino acid sequence shown in SEQ ID NO: 1 in plants in conjunction with a hexadecimal fatty acid-specific thioesterase and a Z11 desaturase can synthesize (Z)-13-octadecenoic acid. The process of synthesizing (Z)-13-octadecenoic acid using plants is green and pollution-free, purification is simple and convenient, environmental pollution is low, and the extraction purity is high.

The present application provides a protein and a method for synthesizing an insect sex pheromone precursor compound. The present application finds that co-expressing a protein with a hexadecanoic fatty acid specific thioesterase in plants, which amino acid sequence is shown in SEQ ID No. 2, can synthesize 10-hexadecenoic acid. The process of synthesizing 10-hexadecenoic acid by plants is green and pollution-free, has low environmental pollution degree due to simple and convenient purification, and has high purity of extraction, which changes the path of relying on chemical synthesis, solves the disadvantages of complex chemical synthesis, large amount of by-products, environmental pollution, high cost and low purity of final product, etc.

The present invention relates to a preparation method of ascorbyl tetraisopalmitate, and belongs to the technical field of bioenzymatic synthesis. The preparation method of the present invention comprises the following steps: S1 fixes a fat synthase complex containing a mixture of malonyl-CoA, β-ketoacyl-ACP synthase and palmitoyl-ACP thioesterase in a fixative to obtain a solid biological fat synthase complex; malonic acid and the solid biological fat synthase complex are catalyzed in a saturated sodium bicarbonate aqueous solution to obtain palmitic acid; S2 esterifies a solution of palmitic acid with ascorbyl glyceride, methyl silicate and immobilized lipase to obtain ascorbyl tetraisopalmitate. The present invention uses sustainable and recyclable malonate as a substrate and utilizes an immobilized fat synthase complex to prepare palmitic acid more quickly and sustainably; ascorbyl tetraisopalmitate is prepared by esterifying ascorbyl glyceride with palmitic acid, providing a preparation method with high product purity and sustainable raw material sources.

The present disclosure provides polypeptides (e.g., TE polypeptides), polynucleotides and expression vectors and host cells comprising the same, methods of producing polypeptides (e.g., TE polypeptides), and methods of catalyzing an amide bond formation.

A method of modifying fatty acid profiles of canola (Brassica napus) oil are provided. In some embodiments, the method comprises providing a canola plant having a genetic construct in its genome, the genetic construct comprising coding sequences for Umbellularia californica C12:0 specific acyl-ACP thioesterase and Cinnamomum camphora C14:0 specific acyl-ACP thioesterase operatively linked to seed specific promoters. Also provided are genetically modified canola plants, plant cells, and seeds as well as canola oil extracted therefrom. The canola oils have a balanced fatty acid profile that provides health benefits as well as improved oxidative stability over conventional canola oils.

This application relates to the technical field of *Zymomonas mobilis*, specifically to an engineered strain of *Zymomonas mobilis*, a preparation method thereof, and an application thereof. The engineered strain uses *Zymomonas mobilis* as the starting strain, and an exogenous 3-hydroxybutyric acid (3HB) synthesis pathway is introduced to obtain a strain with the ability to produce 3HB. Furthermore, by screening for highly efficient thioesterases in the 3HB synthesis pathway, increasing the copy number of 3HB synthesis genes, overexpressing genes related to cofactor supply, introducing an exogenous ethanol utilization pathway, constructing a penicillin-binding protein (PBPs)-deficient strain, optimizing the culture medium, and other systems of metabolic engineering and fermentation condition optimization, the 3HB yield is further increased to obtain a recombinant strain with high 3HB production and a 3HB preparation method. This *Zymomonas mobilis* has application prospects such as highly efficient biosynthesis of 3HB and biodegradable polymers.