13 results about "Thioesterase" patented technology

This invention provides fatty diols and recombinant microorganisms for producing them. [Solution] A recombinant microorganism for producing 1,3-lipid diols when grown in a fermentation broth containing a simple carbon source, wherein the recombinant microorganism is engineered to express a nucleic acid sequence encoding a polypeptide that includes (a) thioesterase (EC3.1.2.-, EC3.1.1.5, or EC3.1.2.14) activity and (b) carboxylic acid reductase (EC6.2.1.3 or EC1.2.1.42) activity.

Recombinant cells and methods for producing methyl ketones, such as medium-chain methyl ketones. The recombinant cells include recombinant acyl-ACP thioesterase genes, recombinant β-ketoacyl-CoA thioesterase genes, and recombinant acyl-CoA synthetase genes, in addition to other modifications. The methods include culturing the recombinant cells to produce the methyl ketones and isolating the produced methyl ketones.

This invention discloses a tomato thioesterase gene. SlFATB2 and its application in regulating tomato growth, development, and disease resistance, the aforementioned SlFATB2 The gene has the nucleotide sequence shown in SEQ ID No. 1. Its CDS sequence was cloned using bioinformatics combined with molecular biology techniques, and a strong promoter was constructed. SlFATB2 Overexpression vectors were used to obtain transgenic tomato plants overexpressing the vectors. The CRISPR / Cas9 knockout system was used to obtain... SlFATB2 Gene knockout tomato transgenic lines. Experiments have confirmed that knockout... SlFATB2 This leads to pollen shrinkage, fruit and seed shrinkage, and susceptibility to gray mold; overexpression SlFATB2 It can enhance resistance to gray mold without affecting normal growth. This invention clarifies that... SlFATB2 The gene function can be precisely improved in tomato agronomic traits and disease resistance through gene editing or overexpression, and its application prospects are broad.

This invention relates to a protein and method for synthesizing cis-11-tetradecenoic acid. The invention discovers that cis-11-tetradecenoic acid can be synthesized by co-expressing a protein with the amino acid sequence shown in SEQ ID No. 2 and a fourteen-carbon fatty acid-specific thioesterase (TE14) in plants. The process of synthesizing cis-11-tetradecenoic acid from plants is green and pollution-free. Due to its simple and convenient purification, it has a low degree of environmental pollution and high purity. This changes the reliance on chemical synthesis and solves the drawbacks of chemical synthesis, such as complexity, large amounts of byproducts, environmental pollution, high cost, and low purity of the final product.

This application relates to the field of genetically engineered bacteria technology, specifically disclosing a genetically engineered *E. coli* strain for producing emodin, its construction method, and its applications. This application uses *E. coli* BAP1 as the substrate strain. This strain carries its own sfp in its genome, which can be used to activate polyketide synthase. Furthermore, *E. coli* itself grows rapidly, has a vigorous metabolism, and the culture medium is low-cost, facilitating high-density fermentation. Through gene editing, the uptake and utilization of glucose are improved, the flux of acetic acid and lactic acid diverted from pyruvate is reduced, and the ATP supply is enhanced to increase the flux of acetyl-CoA and malonyl-CoA within the bacteria. This provides sufficient substrate for the synthesis of emodin by polyketide synthase, thioesterase, and decarboxylase, thereby increasing the yield of emodin. The emodin yield achieved by this application through shake-flask fermentation reaches 576.4 mg / L, which is currently the highest level in the *E. coli* system.

A method for improving the synthetic ability of *Yersinia lipolytica* to synthesize amino acid derivatives. This invention discloses an engineered strain for the biological synthesis of the amino acid derivative β-hydroxy-β-methylbutyric acid (β-methylbutyric acid) and its construction method. Using *Yersinia lipolytica* as the substrate cell, the HMGCL and OCT genes involved in branched-chain amino acid metabolism in its genome are knocked out to obtain a gene knockout strain. Decarboxylase genes, hydratase genes, dehydratase genes, and thioesterase genes are integrated into the genome of the gene knockout strain to obtain an engineered strain capable of de novo synthesis of β-hydroxy-β-methylbutyric acid. Further integration of the CcGCTB-CcGCTA fusion gene expression module enhances the expression level of key enzyme genes, thereby increasing the yield of β-hydroxy-β-methylbutyric acid in the strain. The engineered strain constructed in this invention can synthesize the target product β-hydroxy-β-methylbutyric acid using glucose as a carbon source through fermentation, with a maximum yield of up to 30 g / L.

The invention relates to the technical field of gene engineering, in particular to a method for producing an insect sex pheromone precursor by utilizing rape and application. The invention provides a method for biosynthesizing an insect sex pheromone precursor (Z)-11-hexadecenoic acid in oilseed rape. The method comprises the step of co-expressing a gene for coding hexadecanoic fatty acid specific thioesterase and at least one gene for coding Z11 desaturase in the oilseed rape. The technical breakthrough of efficiently synthesizing the insect sex pheromone precursor in the rape seeds is realized. According to the invention, the insect sex pheromone key precursor (Z)-11-hexadecenoic acid is successfully heterologously synthesized in the rape seeds for the first time, and the content of the Z)-11-hexadecenoic acid accounts for about 24.5% of the total fatty acid of the seeds and is obviously higher than about 20% reported in camelina sativa.

Disclosed is an inhibitor of the acyl-protein thioesterase APT1, ML-348, of formula A:for use in the treatment and / or prevention of a disease or disorder in a subject in need thereof. In particular the inhibitor is for use in the treatment and / or prevention of a neurodegenerative disease or disorder, particularly Huntington's disease. Also disclosed is a pharmaceutical composition including at least the APT1 inhibitor and at least one pharmaceutically acceptable excipient. The inhibitor or composition is intended for use as a medicament. Furthermore, the inhibitor or composition is intended for use in the treatment and / or prevention of a disease or disorder related to impaired intracellular trafficking between the endoplasmic reticulum and the Golgi apparatus and between the latter and the plasma membrane.

When employed, a recombinant expression vector carrying a daptomycin production gene including a foreign fatty acyl AMP ligase (FAAL) gene, a thioesterase (TE) gene, and a fatty acid synthase (FAS) gene according to the present invention can enhance specificity for decanoic acid, and produce daptomycin without supplementation of lipopeptides, such as decanoic acid, from the outside of the strain. In addition, no by-products are generated during the production process, thereby enabling production of daptomycin with high purity and high yield.

The present application provides a protein and a method for synthesizing an insect sex pheromone precursor compound. The present application finds that co-expressing a protein with a hexadecanoic fatty acid specific thioesterase in plants, which amino acid sequence is shown in SEQ ID No. 2, can synthesize 10-hexadecenoic acid. The process of synthesizing 10-hexadecenoic acid by plants is green and pollution-free, has low environmental pollution degree due to simple and convenient purification, and has high purity of extraction, which changes the path of relying on chemical synthesis, solves the disadvantages of complex chemical synthesis, large amount of by-products, environmental pollution, high cost and low purity of final product, etc.

The present disclosure provides polypeptides (e.g., TE polypeptides), polynucleotides and expression vectors and host cells comprising the same, methods of producing polypeptides (e.g., TE polypeptides), and methods of catalyzing an amide bond formation.

Recombinant proteobacteria, including γ-proteobacteria, comprising a heterologous acyl-ACP desaturase and a heterologous acyl-ACP thioesterase, wherein the native dual 3-hydroxy acyl-ACP dehydratase / isomerase is deleted are provided herein. The recombinant proteobacteria produce non-native monounsaturated free fatty acids or derivatives thereof. Methods of producing non-native monounsaturated free fatty acids or derivatives thereof are also provided, in addition to cell cultures and fatty acid compositions produced by the recombinant proteobacteria. The recombinant proteobacteria may be used to produce insect pheromones or precursors thereof, and fragrances or precursors thereof.

The present application relates to the technical field of genetic engineering, and particularly relates to a gene for producing (Z)-9-tetradecenoic acid by using rape and application thereof.The present application provides a method for biosynthesizing insect sex pheromone precursor (Z)-9-tetradecenoic acid in rape, which comprises co-expressing a gene encoding tetradecanoic fatty acid specific thioesterase and at least one gene encoding Z9 desaturase in rape.The present application realizes a technical breakthrough of efficiently synthesizing insect sex pheromone precursor in rape seeds, and successfully synthesizes insect sex pheromone key precursor (Z)-9-hexadecenoic acid in rape seeds for the first time.