27 results about "Thioesterase" patented technology

The invention relates to the technical field of genetic engineering, in particular to a gene for biosynthesis of (Z)-13-octadecenoic acid and application of the gene. The invention discloses a gene for biosynthesis of (Z) 13 octadecenoic acid. An amino acid sequence coded by the gene is as shown in SEQ ID NO: 1. The invention finds that the (Z)-13-octadecenoic acid can be synthesized by jointly expressing the protein with the amino acid sequence shown as SEQ ID NO: 1, hexadecanoic acid specific thioesterase and Z11 desaturase in plants. The process of synthesizing the (Z)-13-octadecenoic acid by using plants is green and pollution-free, the purification is simple and convenient, the environmental pollution degree is low, and the extraction purity is high.

The invention relates to the technical field of enzymology, in particular to a gene for biosynthesis of (Z)-9-hexadecenoic acid and application of the gene. The invention discloses a gene for biosynthesis of (Z)-9-hexadecenoic acid. A nucleotide sequence coded by the gene is shown as SEQ ID NO: 1. The gene and hexadecanoic acid specific thioesterase are jointly expressed in plants, so that the biosynthesis yield of (Z)-9-hexadecenoic acid can be increased. The process of synthesizing the (Z)-9-hexadecenoic acid by using plants is green and pollution-free, and the purification is simple and convenient, so that the environmental pollution degree is low, and the extraction purity is high, thereby changing the path depending on chemical synthesis, and solving the defects of complex chemical synthesis, large by-product amount, environmental pollution, high cost, low final product purity and the like.

The invention provides a method for improving the molecular weight of polyhydroxyalkanoates (polyhydroxyalkanoates, called PHA for short) synthesized by microorganisms, and relates to a method for improving the molecular weight of the polyhydroxyalkanoates (polyhydroxyalkanoates, called PHA for short). By knocking out or knocking down endogenous (intracellular) carboxylesterase, lipase, esterase, thioesterase and PHA degrading enzyme genes of bacteria for synthesizing PHA, the molecular weight of various synthesized PHA is obviously improved. The method has universality for improving the molecular weight of various PHAs synthesized by different species of bacteria, the mechanical property of the PHA can be improved by improving the molecular weight of the PHA, and new performance is provided for expanding the application of the PHA.

The present invention provides a microorganism capable of producing at least one C6-C26 free fatty acid or a mixture thereof from methanol, said microorganism comprising at least one gene enabling the expression of a thioesterase (TE) and at least one gene enabling the expression of at least one Fatty Alcohol Reductase (FAR). The present invention further provides methods for the manufacture of at least one C6-C26 free fatty acids or mixtures thereof and C6-C26 free fatty alcohols or mixtures thereof.

This invention provides fatty diols and recombinant microorganisms for producing them. [Solution] A recombinant microorganism for producing 1,3-lipid diols when grown in a fermentation broth containing a simple carbon source, wherein the recombinant microorganism is engineered to express a nucleic acid sequence encoding a polypeptide that includes (a) thioesterase (EC3.1.2.-, EC3.1.1.5, or EC3.1.2.14) activity and (b) carboxylic acid reductase (EC6.2.1.3 or EC1.2.1.42) activity.

Recombinant cells and methods for producing methyl ketones, such as medium-chain methyl ketones. The recombinant cells include recombinant acyl-ACP thioesterase genes, recombinant β-ketoacyl-CoA thioesterase genes, and recombinant acyl-CoA synthetase genes, in addition to other modifications. The methods include culturing the recombinant cells to produce the methyl ketones and isolating the produced methyl ketones.

The present application relates to a kind of containing fatty acyl-coa dehydrogenase engineering bacteria and its application method, belong to microbial fermentation technical field.The engineering bacteria containing fatty acyl-coa dehydrogenase gene in the present application include fatty acyl-coa dehydrogenase gene and fatty acyl-coa thioesterase gene, the fatty acyl-coa dehydrogenase gene is derived from Candida tropicalis or Yarrowia lipolytica, its nucleotide sequence is SEQ ID NO.1 or SEQ ID NO.2;The nucleotide sequence of the fatty acyl-coa thioesterase gene is SEQ ID NO.3.Fatty acyl-coa dehydrogenase from Candida tropicalis and Yarrowia lipolytica, combined with existing fatty acyl-coa thioesterase, jointly convert 10-hydroxy decanoic acid, significantly improve the conversion rate of 10-hydroxy-2-decenoic acid, so that the industrialization production of 10-hydroxy-2-decenoic acid becomes possible.

The present invention provides a microorganism capable of producing at least one C6-C26 free fatty acid or a mixture thereof from methanol, said microorganism comprising at least one gene enabling the expression of a thioesterase (TE) and at least one gene enabling the expression of at least one Fatty Alcohol Reductase (FAR). The present invention further provides methods for the manufacture of at least one C6-C26 free fatty acids or mixtures thereof and C6-C26 free fatty alcohols or mixtures thereof.

This invention discloses a tomato thioesterase gene. SlFATB2 and its application in regulating tomato growth, development, and disease resistance, the aforementioned SlFATB2 The gene has the nucleotide sequence shown in SEQ ID No. 1. Its CDS sequence was cloned using bioinformatics combined with molecular biology techniques, and a strong promoter was constructed. SlFATB2 Overexpression vectors were used to obtain transgenic tomato plants overexpressing the vectors. The CRISPR / Cas9 knockout system was used to obtain... SlFATB2 Gene knockout tomato transgenic lines. Experiments have confirmed that knockout... SlFATB2 This leads to pollen shrinkage, fruit and seed shrinkage, and susceptibility to gray mold; overexpression SlFATB2 It can enhance resistance to gray mold without affecting normal growth. This invention clarifies that... SlFATB2 The gene function can be precisely improved in tomato agronomic traits and disease resistance through gene editing or overexpression, and its application prospects are broad.

The invention discloses a TE enzyme mutant with improved activity and application thereof, and belongs to the technical field of biology. Schrodinger molecular docking is combined with fixed-point saturation mutation to establish a mutant library, Rosetta is used for screening the mutant library, searching a target point and carrying out single-point mutation and combined mutation, the activity of thioesterase is improved, the synthesis efficiency of Surfactin is further improved, and a production strain with better fermentation performance in industrial production is obtained. Through verification, mutations comprise A113W, A113G, K122H, K192D and a combination of the A113W, the A113G, the K122H and the K192D, fermentation verification shows that the yield of the Surfactin is increased by 41.2% to the maximum compared with that of a wild type and reaches 9.6 g / L, thioesterase with higher activity and a production strain with better fermentation performance in industrial production are obtained, and powerful support is provided for efficient production of the Surfactin.

This invention relates to a protein and method for synthesizing cis-11-tetradecenoic acid. The invention discovers that cis-11-tetradecenoic acid can be synthesized by co-expressing a protein with the amino acid sequence shown in SEQ ID No. 2 and a fourteen-carbon fatty acid-specific thioesterase (TE14) in plants. The process of synthesizing cis-11-tetradecenoic acid from plants is green and pollution-free. Due to its simple and convenient purification, it has a low degree of environmental pollution and high purity. This changes the reliance on chemical synthesis and solves the drawbacks of chemical synthesis, such as complexity, large amounts of byproducts, environmental pollution, high cost, and low purity of the final product.

This application relates to the field of genetically engineered bacteria technology, specifically disclosing a genetically engineered *E. coli* strain for producing emodin, its construction method, and its applications. This application uses *E. coli* BAP1 as the substrate strain. This strain carries its own sfp in its genome, which can be used to activate polyketide synthase. Furthermore, *E. coli* itself grows rapidly, has a vigorous metabolism, and the culture medium is low-cost, facilitating high-density fermentation. Through gene editing, the uptake and utilization of glucose are improved, the flux of acetic acid and lactic acid diverted from pyruvate is reduced, and the ATP supply is enhanced to increase the flux of acetyl-CoA and malonyl-CoA within the bacteria. This provides sufficient substrate for the synthesis of emodin by polyketide synthase, thioesterase, and decarboxylase, thereby increasing the yield of emodin. The emodin yield achieved by this application through shake-flask fermentation reaches 576.4 mg / L, which is currently the highest level in the *E. coli* system.

A method for improving the synthetic ability of *Yersinia lipolytica* to synthesize amino acid derivatives. This invention discloses an engineered strain for the biological synthesis of the amino acid derivative β-hydroxy-β-methylbutyric acid (β-methylbutyric acid) and its construction method. Using *Yersinia lipolytica* as the substrate cell, the HMGCL and OCT genes involved in branched-chain amino acid metabolism in its genome are knocked out to obtain a gene knockout strain. Decarboxylase genes, hydratase genes, dehydratase genes, and thioesterase genes are integrated into the genome of the gene knockout strain to obtain an engineered strain capable of de novo synthesis of β-hydroxy-β-methylbutyric acid. Further integration of the CcGCTB-CcGCTA fusion gene expression module enhances the expression level of key enzyme genes, thereby increasing the yield of β-hydroxy-β-methylbutyric acid in the strain. The engineered strain constructed in this invention can synthesize the target product β-hydroxy-β-methylbutyric acid using glucose as a carbon source through fermentation, with a maximum yield of up to 30 g / L.

The invention provides a protein and a method for synthesizing an insect sex pheromone precursor compound. The invention finds that 10-hexadecenoic acid can be synthesized by jointly expressing the protein with the amino acid sequence shown as SEQ ID No. 2 and hexadecanoic acid specific thioesterase in plants. The process of synthesizing the 10-hexadecenoic acid by using plants is green and pollution-free, and the purification is simple and convenient, so that the degree of environmental pollution is low, and the extraction purity is high, thereby changing the path depending on chemical synthesis, and solving the defects of complex chemical synthesis, large by-product amount, environmental pollution, high cost, low final product purity and the like.

The invention relates to the technical field of gene engineering, in particular to a method for producing an insect sex pheromone precursor by utilizing rape and application. The invention provides a method for biosynthesizing an insect sex pheromone precursor (Z)-11-hexadecenoic acid in oilseed rape. The method comprises the step of co-expressing a gene for coding hexadecanoic fatty acid specific thioesterase and at least one gene for coding Z11 desaturase in the oilseed rape. The technical breakthrough of efficiently synthesizing the insect sex pheromone precursor in the rape seeds is realized. According to the invention, the insect sex pheromone key precursor (Z)-11-hexadecenoic acid is successfully heterologously synthesized in the rape seeds for the first time, and the content of the Z)-11-hexadecenoic acid accounts for about 24.5% of the total fatty acid of the seeds and is obviously higher than about 20% reported in camelina sativa.

Disclosed is an inhibitor of the acyl-protein thioesterase APT1, ML-348, of formula A:for use in the treatment and / or prevention of a disease or disorder in a subject in need thereof. In particular the inhibitor is for use in the treatment and / or prevention of a neurodegenerative disease or disorder, particularly Huntington's disease. Also disclosed is a pharmaceutical composition including at least the APT1 inhibitor and at least one pharmaceutically acceptable excipient. The inhibitor or composition is intended for use as a medicament. Furthermore, the inhibitor or composition is intended for use in the treatment and / or prevention of a disease or disorder related to impaired intracellular trafficking between the endoplasmic reticulum and the Golgi apparatus and between the latter and the plasma membrane.

The present invention provides a microorganism capable of producing at least one C6-C26 free fatty alcohol or a mixture thereof from methanol, said microorganism comprising at least one gene enabling the expression of a thioesterase (TE), at least one gene enabling the expression of at least one carboxylic acid reductase (CAR) and at least one gene enabling the expression of an aldehyde reductase (ALDR). The present invention further provides methods for the manufacture of C6-C26 free fatty alcohols or mixtures thereof, as well as a free fatty alcohol composition obtained from these methods.

When employed, a recombinant expression vector carrying a daptomycin production gene including a foreign fatty acyl AMP ligase (FAAL) gene, a thioesterase (TE) gene, and a fatty acid synthase (FAS) gene according to the present invention can enhance specificity for decanoic acid, and produce daptomycin without supplementation of lipopeptides, such as decanoic acid, from the outside of the strain. In addition, no by-products are generated during the production process, thereby enabling production of daptomycin with high purity and high yield.

This invention relates to the field of genetic engineering technology, and more particularly to a gene for the biosynthesis of (Z)-13-octadecenoic acid and its application. A gene for the biosynthesis of (Z)-13-octadecenoic acid is provided, the amino acid sequence of which is shown in SEQ ID NO: 1. This invention discovers that expressing a protein with the amino acid sequence shown in SEQ ID NO: 1 in plants in conjunction with a hexadecimal fatty acid-specific thioesterase and a Z11 desaturase can synthesize (Z)-13-octadecenoic acid. The process of synthesizing (Z)-13-octadecenoic acid using plants is green and pollution-free, purification is simple and convenient, environmental pollution is low, and the extraction purity is high.

The present application provides a protein and a method for synthesizing an insect sex pheromone precursor compound. The present application finds that co-expressing a protein with a hexadecanoic fatty acid specific thioesterase in plants, which amino acid sequence is shown in SEQ ID No. 2, can synthesize 10-hexadecenoic acid. The process of synthesizing 10-hexadecenoic acid by plants is green and pollution-free, has low environmental pollution degree due to simple and convenient purification, and has high purity of extraction, which changes the path of relying on chemical synthesis, solves the disadvantages of complex chemical synthesis, large amount of by-products, environmental pollution, high cost and low purity of final product, etc.

The present disclosure provides polypeptides (e.g., TE polypeptides), polynucleotides and expression vectors and host cells comprising the same, methods of producing polypeptides (e.g., TE polypeptides), and methods of catalyzing an amide bond formation.

A method of modifying fatty acid profiles of canola (Brassica napus) oil are provided. In some embodiments, the method comprises providing a canola plant having a genetic construct in its genome, the genetic construct comprising coding sequences for Umbellularia californica C12:0 specific acyl-ACP thioesterase and Cinnamomum camphora C14:0 specific acyl-ACP thioesterase operatively linked to seed specific promoters. Also provided are genetically modified canola plants, plant cells, and seeds as well as canola oil extracted therefrom. The canola oils have a balanced fatty acid profile that provides health benefits as well as improved oxidative stability over conventional canola oils.

Recombinant proteobacteria, including γ-proteobacteria, comprising a heterologous acyl-ACP desaturase and a heterologous acyl-ACP thioesterase, wherein the native dual 3-hydroxy acyl-ACP dehydratase / isomerase is deleted are provided herein. The recombinant proteobacteria produce non-native monounsaturated free fatty acids or derivatives thereof. Methods of producing non-native monounsaturated free fatty acids or derivatives thereof are also provided, in addition to cell cultures and fatty acid compositions produced by the recombinant proteobacteria. The recombinant proteobacteria may be used to produce insect pheromones or precursors thereof, and fragrances or precursors thereof.

The present disclosure provides a synthesis method for itaconate driven by thioesterase. The present disclosure provides a novel method for synthesizing itanonate driven by thioester hydrolysis through experiments illustrated in the examples.

The present application relates to the technical field of genetic engineering, and particularly relates to a gene for producing (Z)-9-tetradecenoic acid by using rape and application thereof.The present application provides a method for biosynthesizing insect sex pheromone precursor (Z)-9-tetradecenoic acid in rape, which comprises co-expressing a gene encoding tetradecanoic fatty acid specific thioesterase and at least one gene encoding Z9 desaturase in rape.The present application realizes a technical breakthrough of efficiently synthesizing insect sex pheromone precursor in rape seeds, and successfully synthesizes insect sex pheromone key precursor (Z)-9-hexadecenoic acid in rape seeds for the first time.

The present application relates to the technical field of enzyme, in particular to a gene for biosynthesis of (Z)-9-hexadecenoic acid and application thereof.A gene for biosynthesis of (Z)-9-hexadecenoic acid, the nucleotide sequence coded by the gene is shown as SEQ ID NO:1.The present application can improve the biosynthesis yield of (Z)-9-hexadecenoic acid by co-expressing the above gene and hexadecanoic acid specific thioesterase in plants.The process of synthesizing (Z)-9-hexadecenoic acid by plants is green and non-polluting, and the degree of environmental pollution is low due to the simple and convenient purification, and the purity of the extracted product is high, which changes the path of relying on chemical synthesis, and solves the disadvantages of chemical synthesis, such as complexity, large amount of by-products, environmental pollution, high cost and low purity of final product.