A method of selectively producing male or female sterile plants

Abstract

The present invention discloses a method of producing male or female sterile plants comprising the steps of: transforming plant material with a polynucleotide encoding at least one enzyme that reacts with a non-toxic plant material to produce a plant-toxic material , regenerating such transformed material into plants, wherein the period of male or female gamete formation and / or maturation is not exceeded, the non-phytotoxic substance is applied to said plants, whereby the non-phytotoxic substance provides the phytotoxic The production of a substance that selectively prevents the formation of said gametes or renders them nonfunctional, wherein the enzyme is preferentially expressed in the male or female reproductive structures, is characterized in that: (i) non-toxic phytotoxicity From: Ester derivatives of non-phosphonate herbicides of direct phytotoxicity to non-green tissues, D-alpha amino acids, peptide derivatives of non-protein D-alpha amino acids, S-para to aryloxyphenoxypropionic acids enantiomer and S-enantiomer of ester derivatives of aryloxyphenoxypropionic acid, and (ii) enzyme selected from: carboxylesterase, D-amino acid oxidase, D-amino acid dehydrogenase, D-amino acid Racemase, 2-arylpropionyl-CoA epimerase, alpha-formyl-CoA racemase, thioesterase and acyl-CoA synthetase.

Description

Background technique [0001] Heterosis in crop plants has a significant effect on yield enhancement. Generally, hybrids show increased yield compared to non-hybrid varieties. Hybrids generally give more return units to growth factors such as water and fertilizers. Hybrids often offer superior stress tolerance, yield and consistency of maturity, and also provide simple breeding opportunities to combine traits or traits that would otherwise be difficult to combine. Often, heterosis in plants is important enough to warrant commercial exploitation. Commercial hybrids are widely used in many crops including corn, sorghum, sugar beet, sunflower and canola. However, wheat, barley and rice have mainly been bred by inbreeding mainly due to the lack of methods for the production of economical hybrid seeds. [0002] Traditionally, hybrid seed production involves planting spaced apart plots of female and male parental lines, with only the female parent's seed harvested. To ensure that...

AI Technical Summary

Problems solved by technology

[0010] Despite these methods of producing conditionally sterile male and female parental lines, hybrid seed production is far from routine in crops such as wheat

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