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74 results about "Gamete" patented technology

A gamete (/ˈɡæmiːt/; from Ancient Greek γαμετή gamete from gamein "to marry") is a haploid cell that fuses with another haploid cell during fertilization in organisms that sexually reproduce. In species that produce two morphologically distinct types of gametes, and in which each individual produces only one type, a female is any individual that produces the larger type of gamete—called an ovum— and a male produces the smaller tadpole-like type—called a sperm. In short a gamete is an egg cell (female gamete) or a sperm (male gamete). This is an example of anisogamy or heterogamy, the condition in which females and males produce gametes of different sizes (this is the case in humans; the human ovum has approximately 100,000 times the volume of a single human sperm cell). In contrast, isogamy is the state of gametes from both sexes being the same size and shape, and given arbitrary designators for mating type. The name gamete was introduced by the Austrian biologist Gregor Mendel. Gametes carry half the genetic information of an individual, one ploidy of each type, and are created through meiosis.

Method for separating, purifying and transplanting spermatogonial stem cells of lateolabrax japonicus

The invention discloses a method for separating, purifying and transplanting spermatogonial stem cells of lateolabrax japonicus, the spermatogonial stem cells of lateolabrax japonicus are separated and purified, marine fish larvae or freshwater fish larvae are used as receptor larvae, and then the spermatogonial stem cells of lateolabrax japonicus are transplanted to abdominal cavities of the receptor larvae. The spermatogonial stem cells obtained through separation are complete in number, structure and function and low in cell clustering rate. After the transplanted spermatogonial stem cells are subjected to fluorescence labeling, the migration and proliferation processes of donor germ cells can be observed in receptor fishes of marine fish fries or freshwater fish fries, so that the receptor fishes with the chimeric germline are obtained. According to the method, through xenotransplantation of the spermatogonial stem cells, a'belly borrowing reproduction 'breeding mode is provided for the marine fishes, a new way for gamete preservation and rapid propagation is provided for the marine fishes with the long reproductive cycle, and a new method and a new thought are provided for genetic improvement and germplasm resource preservation.
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

Cloning of male gamete killer gene S19g-A1 of rice interspecific hybrid sterility S19 seat and application of male gamete killer gene S19g-A1

The invention relates to a male gamete killer gene S19g-A1 of a rice interspecific hybrid sterile S19 seat and application of the male gamete killer gene S19g-A1. The invention provides a rice hybrid sterility related protein S19g-A1. The amino acid sequence of the rice hybrid sterility related protein S19g-A1 is as shown in SEQ ID NO. 2. According to the invention, a key gene S19g-A1 of a rice interspecific hybrid sterile S19 site is researched and identified, function knockout is carried out on African rice S19g-A1, and pollen fertility of a mutant hybrid F1 obtained by carrying out distant hybridization on an obtained African rice s19g-a1 mutant returns to normal. Based on a gene editing technology, the invention develops a method for rapidly creating a hybrid affinity line capable of overcoming sterility of African rice hybrid, effectively eliminates S19 locus mediated interspecific hybrid reproductive disorder, breaks through the bottleneck of rice interspecific hybrid advantage utilization, provides target gene resources for distant hybrid advantage utilization and crop yield improvement, and has a wide application prospect. Good application prospects are realized.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Artificial cross breeding method for male epinephelus fuscoguttatus and female humpback perch

The invention relates to an artificial cross breeding method for male epinephelus fuscoguttatus and female humpback perch, which belongs to the technical field of distant cross breeding and comprises the steps of parent selection of the epinephelus fuscoguttatus and the humpback perch, parent ripening cultivation, gamete collection, artificial insemination and incubation and fry cultivation. By means of reproductive regulation and distant hybridization technologies, two species of two genera of grouper family in different geographical distribution and different reproduction time are hybridized, namely cross-generic hybridization of grouper family breaks through the generic reproductive isolation, and therefore good varieties which grow fast and are high in survival rate are cultivated.
Owner:HAINAN BLUE GRAIN TECH CO LTD

Simultaneous gene editing and haploid induction

The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.
Owner:SYNGENTA CROP PROTECITON AG

Complete-cycle nutrition-enhanced goat hybridization breeding method

The invention relates to the technical field of livestock and poultry breeding, in particular to a goat hybridization breeding method capable of achieving complete-cycle nutrition enhancement. The core of the method is that systematic nutrition intervention runs through the whole breeding process. Before hybridization, performing differential nutrition pretreatment on the parents for 60 days: feeding the female parents with a breeding nutrition enhancer added with components such as D-chiro-inositol and N-acetylcysteine, and supplementing sperm motility enhancer containing acetylated L-carnitine and ergothioneine to the male parents. In the gamete treatment stage, the oocytes are subjected to in-vitro maturation culture in a special culture solution containing components such as D-chiro-inositol and a growth differentiation factor 9; after the sperms are subjected to gradient centrifugal screening, completing in-vitro fertilization in an optimized fertilization culture solution; after the embryos develop into blastocysts in the sequential culture system, carrying out laparoscopic transplantation, and carrying out staged precise nutrition culture on the pregnant ewes. According to the method, through multi-level nutrition enhancement, the reproductive capacity of the goats and the growth performance of offspring are effectively improved.
Owner:XINJIANG ACAD OF ANIMAL SCI

System and regulation method for directionally differentiating chicken primordial germ cells into gametes in vitro

The invention discloses a system for directionally differentiating chicken primordial germ cells into gametes in vitro and a regulation and control method, the system comprises a basic culture medium: based on DMEM / F12, adding 10% of fetal calf serum, 2% of chicken serum, 2 mmol / L of L-glutamine, 1 mmol / L of sodium pyruvate and 5.5 * 10 <-5 > mol / L of beta-mercaptoethanol; the spermatogonium induction culture medium further comprises BMP4, RA and GDNF; the oogonium induction culture medium also comprises BMP15, GDF9 and EGF (Epidermal Growth Factor). According to the system and the regulation and control method, the differentiation efficiency is improved, the culture period is shortened, and the number of cell clusters is remarkably increased.
Owner:YANGZHOU UNIV

Embryogenesis factors for cellular reprogramming of a plant cell

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing embryogenesis factor genes and / or morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing embryogenesis factor gene products and / or morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Embryogenesis factor proteins and / or developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.
Owner:PIONEER HI BREED INTERNATIONAL INC

A method for detecting single-cell gene sequence variation in rice and its application

The present invention provides a method for detecting single-cell gene sequence variation in rice and its application, which belongs to the field of agricultural technology. The method of the present invention mainly includes three aspects: sampling, sequencing and bioinformatics analysis, wherein the sampling is to obtain a single rice microspore sex cell. The present invention successfully isolates a single rice microspore sex cell, and improves the defects of the existing rice single-cell variation detection technology process, such as weak integrity and detection consistency that need to be improved. In addition, the authenticity of the mutation site is identified and confirmed by heavy ion mutagenesis technology. The method of the present invention broadens the source range of rice single-cell mutagenic materials, and provides new ideas and methods for creating more diverse breeding materials and conducting more efficient mutagenic breeding. At the same time, microspore sex cells serve as male gametes, and the mutations carried in the genome can be passed into the offspring through hybridization, which can realize the inheritance and fixation of mutations, and also provide a way to quickly utilize excellent mutant genes.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY +1

Pollen lethal module based on soybean alpha-amylase gene and application thereof

The invention discloses a soybean alpha-amylase gene-based pollen lethal module and application thereof, the soybean alpha-amylase gene-based pollen lethal module comprises a pollen specific promoter, a plastid leader peptide gene and a soybean alpha-amylase gene, and the pollen specific promoter, the plastid leader peptide gene and the soybean alpha-amylase gene are sequentially connected in a direction from 5'to 3 ', the plastid leader peptide gene and the soybean alpha-amylase gene form a fusion gene, the pollen specific promoter is a soybean GmPG031 gene promoter, the nucleotide sequence of the pollen specific promoter is as shown in SEQ ID NO: 1, the soybean alpha-amylase gene is driven by the pollen specific promoter to be directionally expressed in a male gamete, and the plastid leader peptide gene is combined to guide zymoprotein to amyloid plastid, so that the soybean alpha-amylase gene is obtained. Starch reserve in the pollen is specifically consumed, so that the pollen carrying the module is aborted due to energy failure, the mechanism realizes accurate death of the transgenic pollen, a basic element is provided for a soybean seed production technology, and a core tool is provided for large-scale creation of a male sterile line.
Owner:NORTHWEST UNIV

A method for creating polyploid fish by knocking out the spo11 gene

The application discloses a method for creating polyploid fish, which comprises the following steps of: knocking out a spo11 gene by using a CRISPR / Cas9 gene knockout technology, obtaining a spo11 homozygous knockout individual through genotyping and breeding, utilizing the characteristic that a spo11 homozygous knockout diploid female can produce part of unmeiotic oocytes to obtain a triploid, and further screening a spo11 homozygous knockout triploid 3N spo11 ‑ / ‑ / ‑ ; utilizing the characteristic that a 3N spo11 ‑ / ‑ / ‑ female can produce part of unmeiotic oocytes to further screen a heterozygous knockout tetraploid 4N spo11 + / ‑ / ‑ / ‑ ; utilizing the characteristic that a 4N spo11 + / ‑ / ‑ / ‑ can produce a large number of diploid gametes to further prepare a large number of triploid offspring. Compared with other polyploid breeding methods, the application has universality and genetic controllability, and provides important technical support for artificially inducing fish polyploidy.
Owner:HUAZHONG AGRI UNIV

Application of i4JL in the breeding of wheat near-isogenic lines

ActiveCN120624694BBiotechnologyMale gamete generation
This patent discloses a breeding method for selecting near-isogenic wheat lines using the isoarm chromosome i4JL of *Thinopyrum besarmentosum*. The i4JL chromosome has a unique fluorescent band, making it easily distinguishable from wheat chromosomes. In a wheat background, it is primarily transmitted through female gametes, with extremely low transmission rates in male gametes. Furthermore, it contains two doses of the blue grain gene. Therefore, the self-crossing progeny of the wheat-*Thinopyrum besarmentosum* i4JL monosomatic addition line MAi4JL always exhibits easily separable blue and non-blue grains. By crossing MAi4JL with any non-blue grain wheat and selecting blue grain seeds from the hybrid progeny for five consecutive generations, near-isogenic wheat lines with different backgrounds and phenotypes (blue and non-blue grains) can be obtained, providing a new method for selecting near-isogenic wheat lines. Since i4JL significantly reduces plant height in different wheat backgrounds and significantly affects gene expression in wheat stems and grains, it has significant application potential in wheat genetic breeding research and applications.
Owner:NANJING AGRICULTURAL UNIVERSITY +1

Method for efficiently inducing genomic variation in rice microspore sexual cells

PCT designated stageWO2025245912A1Growth substratesCulture mediaBiotechnologyStaining
A method for efficiently inducing genomic variation in rice microspore sexual cells, relating to the technical field of plant cell engineering. In the method, a rice male gamete (early uninucleate microspore) is used as a mutagenic material, it is attempted, for the first time, to irradiate rice microspore sexual cells with heavy ions (4 Gy) and γ rays (10 Gy), experimental technologies such as fluorescence microscope observation, FDA-PI dyeing, and immunofluorescence detection are taken into account to determine three indicators comprising a target period for irradiation sample sampling, an appropriate heavy ion / γ ray radiation dose, and an appropriate sampling time after the end of radiation, and a relatively complete rice microspore sexual cell mutagenesis system is established. The method involves clear indicators, strong operability, can support batch mutagenesis operations, and can provide a technical system support for the efficient batch production of rice sexual cell mutants.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY +1

Cyclina sinensis sperm freezing preservation method

The invention relates to the technical field of cyclina sinensis sperm preservation, in particular to a cyclina sinensis sperm freezing preservation method. The cyclina sinensis sperm freezing preservation method comprises the following steps: (1) putting parent shellfish into a seawater culture pond, and accelerating ripening for 7-10 days by using a ripening agent; the ripening agent is prepared from a spirulina extract, a radix paeoniae alba extract, a codonopsis pilosula extract, astaxanthin and alginic acid; (2) controlling 0.05-0.15 m / s water flow to scour and stimulate the cyclina sinensis, selecting individuals with sperms discharged after the parent shellfish gametes start to be discharged, and collecting the sperms of the cyclina sinensis; and (3) mixing the cyclina sinensis sperms with a cryoprotectant, pre-cooling, and then cryopreserving for a long time. Research results show that the use of the cryoprotectant can significantly reduce the degree of DNA damage, and the protection effect may be realized through a plurality of mechanisms such as direct combination of trehalose with DNA molecules, free radical removal, maintenance of the stability of a chromatin structure and the like.
Owner:JIANGSU OCEAN UNIV

Method for cultivating new germplasm of three-way hybrid grouper

The invention relates to a three-way hybrid grouper new germplasm cultivation method, and belongs to the technical field of grouper genetic breeding. According to the method, epinephelus moara and golden tiger hybridized fries (female epinephelus fuscoguttatus * male epinephelus coioides) are used as breeding materials, three-way hybridization is carried out in a matching mode of female epinephelus moara * male epinephelus coioides, and breeding of three epinephelus genetic species and fry with excellent characteristics is achieved. The method comprises the steps of parent selection, parent fish reproduction regulation and control, parent fish spawning induction, gamete collection, artificial insemination, incubation and fry rearing. According to the method, on the basis of reproductive regulation and crossbreeding technologies, time and space reproductive isolation of the groupers is broken through, and the new grouper germplasm which is high in survival rate, low in malformation rate and high in growth speed is cultivated.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Simultaneous gene editing and haploid induction

The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.
Owner:SYNGENTA CROP PROTECITON AG

Method for breeding all-male tetraploid crucian carp

The present application relates to fish breeding technology field, especially to a kind of all-male tetraploid crucian carp breeding method, comprising the following steps: with gynogenesis homologous diploid crucian carp as female parent, and androgenesis homologous diploid crucian carp as male parent, artificial delivery is carried out;Wherein, the gynogenesis homologous diploid crucian carp sex chromosome type is XX, produces non-reduction diploid egg, and the androgenesis homologous diploid crucian carp male individual sex chromosome type is XY or YY, produces non-reduction diploid sperm;Artificial insemination is carried out, and the obtained zygote is hatched, and cultured to sexual maturity, and the surviving offspring is all-male tetraploid crucian carp population.The present application is based on the characteristics that gynogenesis homologous diploid crucian carp and androgenesis homologous diploid crucian carp both produce non-reduction gametes, artificial insemination is carried out, and all-male tetraploid crucian carp can be prepared on a large scale.The all-male tetraploid crucian carp only needs 120 days of age to reach sexual maturity, can stably produce diploid sperm, and the reproductive performance is improved.
Owner:HUNAN NORMAL UNIVERSITY

Plant male gamete fertility control method and method for obtaining male sterile material

The invention provides a plant male gamete fertility control method and a method for obtaining a male sterile material, and belongs to the technical field of plant breeding. According to the invention, the PPCD1 is taken as a target gene, and the PPCD1 gene is subjected to expression inhibition or gene knockout, so that the PPCD1 gene loses functions, pollen development fails, and the purpose of fertility control is achieved. Therefore, the male sterility material can be created through the method, and the method can be used for creating male sterility materials in cross breeding, creating materials for preventing genetic escape of transgenic plants, creating plant materials without pollen pollution and the like, so that the production efficiency, the economic effect and the social and environmental effects are greatly improved.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES

A method for isolating, culturing and identifying chicken gonadal somatic cells

This invention discloses a method for the isolation, culture, and identification of chicken sexual gonadal cells, belonging to the field of biotechnology. The invention selects chicken embryos hatched to 18.5 days old, isolates the gonads after PCR sex identification, and uses 0.25% trypsin-EDTA digestion followed by filtration through a 70μm filter to obtain a single-cell suspension with a survival rate >90% and free from PGC contamination. A sex-specific culture system is established, allowing cells to be stably passaged and maintain a sex-specific phenotype. Through morphological, qRT-PCR, immunofluorescence, and Western blot multidimensional identification, accurate identification of male testicular Sertoli cells and female ovarian granulosa cells is achieved. This method is simple to operate, has good reproducibility, and produces high cell viability and purity. It can provide a stable gonadal microenvironment for the in vitro directed differentiation of chicken primordial germ cells, significantly improving gamete induction efficiency. It is suitable for research on avian reproductive and developmental mechanisms, transgenic breeding, and germplasm resource preservation.
Owner:YANGZHOU UNIV

Construction method and application of tilapia mossambica meiosis recombination model based on rec8b gene editing

The invention relates to a construction method and application of a tilapia mossambica meiosis recombination model based on rec8b gene editing, and belongs to the field of gene engineering. The method aims at solving the technical problems that in existing animal meiosis model construction, a physical / chemical induction method is low in polyploidy rate and cannot be inherited, and a gene editing method is accompanied by cell apoptosis and cannot obtain fertile gametes. The invention provides a construction method of a tilapia meiosis recombination model based on rec8b gene editing, which comprises the following steps: designing sgRNA of a targeted rec8b gene exon3, mixing the sgRNA with Cas9mRNA according to a ratio, then microinjecting the mixture into a single cell stage fertilized egg, and carrying out F0 generation backcross and F2 generation screening to obtain a homozygous mutant as a model. The genetic and fertile vertebrate meiosis recombinant living body model is established, and an industrial and scientific research technology platform is provided for meiosis mechanism research and polyploid breeding.
Owner:GERMPLASM INNOVATION GRAND SCIENCE CENTER OF WESTERN CHINA (CHONGQING) SCIENCE CITY +1

Storage and identification device for gametes in a reproductive laboratory

1. The name of the design product: the storage and identification device of sperm and ovum in the reproductive laboratory. 2. The use of the design product: the storage and identification of the test tube containing sperm and ovum in the reproductive laboratory. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best shows the design points: perspective view.
Owner:YUNNAN LEFAN TECH CO LTD

A simple and rapid breeding method for artificially synthesizing wheat

PendingCN122074395AUnleashing breeding potentialPlant genotype modificationBiotechnologyMeiosis
This invention discloses a simple and rapid breeding method for artificially synthesizing wheat. It involves inducing incomplete meiosis in *Gnaphalium affine*, a close relative of wheat, under high red light (R / FR≥1.4) to produce unmeiotic divisions. This method falls under the field of crop breeding technology and includes the following steps: *Gnaphalium affine* (DD) cultured in a high red light (R / FR≥1.4) environment undergoes incomplete meiosis during reproductive growth to produce unmeiotic gametes. These gametes are then used as the male parent and crossed with *Langdon* (AABB). The resulting F1 generation (ABDD) is tetraploid. The F2 generation obtained by self-pollination of this F1 generation is hexaploid (AABBDD). Furthermore, the artificially synthesized hexaploid wheat offspring can be stably inherited.
Owner:TAIAN MAIYUAN SEED IND CO LTD +1

A method for artificial breeding of tropical sea cucumbers

The present invention discloses a method for artificial breeding of tropical sea cucumbers. The method comprises the collection of sea cucumber parents, acquisition of gametes, fertilization and hatching, cultivation of planktonic larvae, attachment and cultivation of larvae, and intermediate culture of tropical sea cucumbers. For the first time, sea cucumbers of the jade foot, sea cucumbers of the brown ring, and sea cucumbers of the mikkelsen are bred by dissecting and obtaining sperm and eggs. The method provides a technical guarantee for the continuation and resource recovery of tropical sea cucumber populations that are on the verge of extinction and cannot undergo artificial induction of spawning. The method is simple and easy to operate for artificial breeding of tropical sea cucumbers, and can effectively overcome a series of problems in the seedling cultivation process, such as the inability to induce spawning / poor induction of spawning effect, unstable output, and unscientific feeding of baits. The success rate of artificial breeding of tropical sea cucumber seedlings is improved. The method does not damage key structures such as the body wall and other organs of the parent organism, and has a very broad application space in the field of artificial breeding and aquaculture of tropical sea cucumbers.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY

Application of H3K9me2 as target spot in preparation of preparation for inducing differentiation of stem cells to gametes in vitro

PendingCN121186373ABiological testingSOX2Meiosis
The invention discloses application of H3K9me2 as a target spot in preparation of a preparation for inducing stem cells to differentiate to gametes in vitro, and relates to the technical field of stem cell breeding in animal husbandry. An H3K9me2 inhibitor BIX01294 is adopted as a treating agent, and in-vivo treatment is performed on a pregnant mouse and in-vitro treatment is performed on gonad tissue of a female fetal mouse. The result shows that the BIX01294 treatment can cause the reduction of the expression level of H3K9me2, so that the expression levels of pluripotent genes Oct4, Sox2 and Dppa3 are increased, the accessibility of whole genome chromatin is obviously changed, and the meiosis start and meiosis process are blocked. The result shows that the H3K9me2 can influence the differentiation of the stem cells to the gametes and is beneficial to the generation of artificial gametes in the stem cell breeding process.
Owner:INNER MONGOLIA UNIVERSITY

An artificial assisted natural reproduction method for eel

The application belongs to the field of aquaculture, and discloses an artificial assisted natural breeding method of eel, aiming at the problems of different development of female and male gonads, difficult control of spawning time, short survival period of initial hatched fry and the like in the artificial breeding technology of eel, the application adjusts the type and dosage of maturation drugs, and combines habitat simulation and other technical means to assist the synchronous maturation of gonads of eel parent fish, promote the natural mating of eel, and solve the defects of the above artificial breeding technology. Compared with the disclosed breeding technology, the application, through the synergistic effect of hormones and the habitat simulation technology, significantly reduces the dosage of pituitary and chorionic gonadotropin while ensuring the maturation effect, reduces the physiological stress of parent fish, and obtains high-quality gametes, so that the synchronous rate of gonad development of eel parent fish is increased from 18% to 66%, the hatching rate of eel fertilized eggs is increased from 48% to 78%, and the survival time of eel fry is prolonged from 6.8 days to 19.8 days.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

Method for improving artificial breeding efficiency of oyster in Hong Kong, China by using riboflavin and application thereof

The present application relates to the field of aquaculture technology, in particular to a method for improving the efficiency of artificial breeding of Hong Kong oyster and application, the present application adopts two ways of treating Hong Kong oyster gametes or parent oysters with riboflavin, which can significantly improve the efficiency of artificial breeding of Hong Kong oyster, and the hatching efficiency of artificial embryos of Hong Kong oyster is significantly improved; through experiments, when the parent oysters of Hong Kong oyster are treated with riboflavin, the final concentration of riboflavin is 3mg / L, the salinity of seawater is 12‰, the hatching rate of oysters is increased from 16.25% of the control group to 82.99%, which is increased by 4.11 times; when the gametes of Hong Kong oyster are treated with riboflavin, the sperm and egg of Hong Kong oyster need to be mixed and soaked in 1mg / L riboflavin seawater solution, and when the salinity of seawater is 12‰, the hatching rate of oysters is increased from 49.78% of the control group to 76.48%, which is increased by 53.64%.
Owner:BEIBU GULF UNIV

Myxocyprinus asiaticus individual genetic resurrection method and application thereof

The invention provides a myxocyprinus asiaticus individual genetic resurgence method which comprises the following steps: S1, taking a myxocyprinus asiaticus gonad tissue, cleaning, cutting into pieces, adding an L-15 culture medium containing double antibodies and a DPBS buffer solution, and centrifuging to obtain the gonad tissue; s2, performing water bath on the gonad tissues to obtain resuscitated gonad tissues; s3, digesting, filtering and centrifugally purifying the resuscitated gonad tissues to obtain germline stem cells; s4, injecting morpholino to obtain gobiocypris rarus without primordial germ cells; and S5, transplanting the germline stem cells into gobiocypris rarus juvenile fish bodies, and culturing to obtain the myxocyprinus asiaticus gametes. S6, the generated myxocyprinus asiaticus gametes are fertilized, and myxocyprinus asiaticus individuals are obtained. According to the method, the sperms of the myxocyprinus asiaticus are successfully obtained by utilizing a gonad cryopreservation and abdomen borrowing reproduction technology, and a feasible way is provided for individual genetic resurgence of rare and endangered fishes.
Owner:INST OF AQUATIC LIFE ACAD SINICA

Improved efficiency of haploid editing

PendingCN122319239AEmbryoGenomic clone
This disclosure relates to the use of heat treatment to increase the haploid editing rate in offspring produced from haploid-induced edited lines. During hybridization, parental gametes fuse to form an embryo; and simultaneously, a gene-editing molecular system is delivered to this embryo. Applying heat treatment to the pollinated plants improves the haploid editing rate. During embryo development, one set of parental chromosomes disappears, and the gene-editing molecular system acts on the remaining set of chromosomes. Thus, hybridization produces at least one haploid offspring with the edited gene.
Owner:SYNGENTA CROP PROTECITON AG +1

Tissue-culture independent gene editing of cells by a long-distance RNA transport system

In an embodiment, the present disclosure relates to a method of editing at least one gene in plant target cells. The method generally includes introducing genetic components of a gene editing system to a first region of the plant. The genetic components are then transported from the first region to the second region, which is different from the first region. The genetic components are processed in the cells in the second region to form the gene editing system such that the gene editing system edits the at least one gene in the cells. The gene edited cells give rise to gametes that produce gene edited seeds upon fertilization.
Owner:TEXAS TECH UNIV SYST

A method for determining heterologous hexaploid imbalance coefficient

The application relates to the technical field of biology, and in particular to a method for determining a heterologous hexaploid imbalance coefficient. The method comprises the following steps: constructing a parent gamete frequency calculation model comprising an HWD coefficient and an LD coefficient; inputting the parent gamete frequency calculation model into a progeny zygote frequency distribution model to obtain a progeny zygote frequency calculation model; constructing a determination model; obtaining actual values of the heterologous hexaploid progeny zygote frequency in a natural population, inputting the actual values of the progeny zygote frequency into the determination model, and determining estimated values of the parent gamete frequency; and performing parameter estimation on the determination model by using a numerical optimization algorithm to obtain determined values of the HWD coefficient and the LD coefficient. The HWD coefficient and the LD coefficient are used to reflect the complex LD network between heterologous hexaploid genomic markers, and the problems that current general polyploid LD estimation does not consider the complex LD network between two-site multiple alleles and that a heterologous hexaploid LD network model is lacking are solved.
Owner:SHANDONG UNIV OF TECH

Module for preparing male gametophyte sterility as well as preparation method and application of module

PendingCN121204130AOxidoreductasesFermentationBiotechnologySporophyte
The invention discloses a module for preparing male gametophyte sterility and a preparation method and application thereof, the module is formed by connecting a pollen specific promoter, an interference element of an OsPTDa gene and an interference element of an OsPTDb gene in series through tRNA-Gly, and the gametophyte male sterility module can be obtained after the elements are assembled. The gametophyte male sterility module disclosed by the invention can down-regulate the expression of male gamete development related genes OsPTDa and OsPTDb at the same time, so that the pollen insemination ability is lost, gametophyte male sterility is generated, and the gametophyte male sterility module plays an important role in preventing transgene from escaping along with pollen, breeding sporophyte male nuclear sterility and the like.
Owner:YUNNAN UNIV