40 results about "Gamete" patented technology

The invention relates to a male gamete killer gene S19g-A1 of a rice interspecific hybrid sterile S19 seat and application of the male gamete killer gene S19g-A1. The invention provides a rice hybrid sterility related protein S19g-A1. The amino acid sequence of the rice hybrid sterility related protein S19g-A1 is as shown in SEQ ID NO. 2. According to the invention, a key gene S19g-A1 of a rice interspecific hybrid sterile S19 site is researched and identified, function knockout is carried out on African rice S19g-A1, and pollen fertility of a mutant hybrid F1 obtained by carrying out distant hybridization on an obtained African rice s19g-a1 mutant returns to normal. Based on a gene editing technology, the invention develops a method for rapidly creating a hybrid affinity line capable of overcoming sterility of African rice hybrid, effectively eliminates S19 locus mediated interspecific hybrid reproductive disorder, breaks through the bottleneck of rice interspecific hybrid advantage utilization, provides target gene resources for distant hybrid advantage utilization and crop yield improvement, and has a wide application prospect. Good application prospects are realized.

The invention relates to an artificial cross breeding method for male epinephelus fuscoguttatus and female humpback perch, which belongs to the technical field of distant cross breeding and comprises the steps of parent selection of the epinephelus fuscoguttatus and the humpback perch, parent ripening cultivation, gamete collection, artificial insemination and incubation and fry cultivation. By means of reproductive regulation and distant hybridization technologies, two species of two genera of grouper family in different geographical distribution and different reproduction time are hybridized, namely cross-generic hybridization of grouper family breaks through the generic reproductive isolation, and therefore good varieties which grow fast and are high in survival rate are cultivated.

The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.

The invention relates to the technical field of livestock and poultry breeding, in particular to a goat hybridization breeding method capable of achieving complete-cycle nutrition enhancement. The core of the method is that systematic nutrition intervention runs through the whole breeding process. Before hybridization, performing differential nutrition pretreatment on the parents for 60 days: feeding the female parents with a breeding nutrition enhancer added with components such as D-chiro-inositol and N-acetylcysteine, and supplementing sperm motility enhancer containing acetylated L-carnitine and ergothioneine to the male parents. In the gamete treatment stage, the oocytes are subjected to in-vitro maturation culture in a special culture solution containing components such as D-chiro-inositol and a growth differentiation factor 9; after the sperms are subjected to gradient centrifugal screening, completing in-vitro fertilization in an optimized fertilization culture solution; after the embryos develop into blastocysts in the sequential culture system, carrying out laparoscopic transplantation, and carrying out staged precise nutrition culture on the pregnant ewes. According to the method, through multi-level nutrition enhancement, the reproductive capacity of the goats and the growth performance of offspring are effectively improved.

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing embryogenesis factor genes and / or morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing embryogenesis factor gene products and / or morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Embryogenesis factor proteins and / or developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

This patent discloses a breeding method for selecting near-isogenic wheat lines using the isoarm chromosome i4JL of *Thinopyrum besarmentosum*. The i4JL chromosome has a unique fluorescent band, making it easily distinguishable from wheat chromosomes. In a wheat background, it is primarily transmitted through female gametes, with extremely low transmission rates in male gametes. Furthermore, it contains two doses of the blue grain gene. Therefore, the self-crossing progeny of the wheat-*Thinopyrum besarmentosum* i4JL monosomatic addition line MAi4JL always exhibits easily separable blue and non-blue grains. By crossing MAi4JL with any non-blue grain wheat and selecting blue grain seeds from the hybrid progeny for five consecutive generations, near-isogenic wheat lines with different backgrounds and phenotypes (blue and non-blue grains) can be obtained, providing a new method for selecting near-isogenic wheat lines. Since i4JL significantly reduces plant height in different wheat backgrounds and significantly affects gene expression in wheat stems and grains, it has significant application potential in wheat genetic breeding research and applications.

The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.

The invention provides a plant male gamete fertility control method and a method for obtaining a male sterile material, and belongs to the technical field of plant breeding. According to the invention, the PPCD1 is taken as a target gene, and the PPCD1 gene is subjected to expression inhibition or gene knockout, so that the PPCD1 gene loses functions, pollen development fails, and the purpose of fertility control is achieved. Therefore, the male sterility material can be created through the method, and the method can be used for creating male sterility materials in cross breeding, creating materials for preventing genetic escape of transgenic plants, creating plant materials without pollen pollution and the like, so that the production efficiency, the economic effect and the social and environmental effects are greatly improved.

This invention discloses a method for the isolation, culture, and identification of chicken sexual gonadal cells, belonging to the field of biotechnology. The invention selects chicken embryos hatched to 18.5 days old, isolates the gonads after PCR sex identification, and uses 0.25% trypsin-EDTA digestion followed by filtration through a 70μm filter to obtain a single-cell suspension with a survival rate >90% and free from PGC contamination. A sex-specific culture system is established, allowing cells to be stably passaged and maintain a sex-specific phenotype. Through morphological, qRT-PCR, immunofluorescence, and Western blot multidimensional identification, accurate identification of male testicular Sertoli cells and female ovarian granulosa cells is achieved. This method is simple to operate, has good reproducibility, and produces high cell viability and purity. It can provide a stable gonadal microenvironment for the in vitro directed differentiation of chicken primordial germ cells, significantly improving gamete induction efficiency. It is suitable for research on avian reproductive and developmental mechanisms, transgenic breeding, and germplasm resource preservation.

1. The name of the design product: the storage and identification device of sperm and ovum in the reproductive laboratory. 2. The use of the design product: the storage and identification of the test tube containing sperm and ovum in the reproductive laboratory. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best shows the design points: perspective view.

This invention discloses a simple and rapid breeding method for artificially synthesizing wheat. It involves inducing incomplete meiosis in *Gnaphalium affine*, a close relative of wheat, under high red light (R / FR≥1.4) to produce unmeiotic divisions. This method falls under the field of crop breeding technology and includes the following steps: *Gnaphalium affine* (DD) cultured in a high red light (R / FR≥1.4) environment undergoes incomplete meiosis during reproductive growth to produce unmeiotic gametes. These gametes are then used as the male parent and crossed with *Langdon* (AABB). The resulting F1 generation (ABDD) is tetraploid. The F2 generation obtained by self-pollination of this F1 generation is hexaploid (AABBDD). Furthermore, the artificially synthesized hexaploid wheat offspring can be stably inherited.

The present application relates to the field of aquaculture technology, in particular to a method for improving the efficiency of artificial breeding of Hong Kong oyster and application, the present application adopts two ways of treating Hong Kong oyster gametes or parent oysters with riboflavin, which can significantly improve the efficiency of artificial breeding of Hong Kong oyster, and the hatching efficiency of artificial embryos of Hong Kong oyster is significantly improved; through experiments, when the parent oysters of Hong Kong oyster are treated with riboflavin, the final concentration of riboflavin is 3mg / L, the salinity of seawater is 12‰, the hatching rate of oysters is increased from 16.25% of the control group to 82.99%, which is increased by 4.11 times; when the gametes of Hong Kong oyster are treated with riboflavin, the sperm and egg of Hong Kong oyster need to be mixed and soaked in 1mg / L riboflavin seawater solution, and when the salinity of seawater is 12‰, the hatching rate of oysters is increased from 49.78% of the control group to 76.48%, which is increased by 53.64%.

The invention provides a myxocyprinus asiaticus individual genetic resurgence method which comprises the following steps: S1, taking a myxocyprinus asiaticus gonad tissue, cleaning, cutting into pieces, adding an L-15 culture medium containing double antibodies and a DPBS buffer solution, and centrifuging to obtain the gonad tissue; s2, performing water bath on the gonad tissues to obtain resuscitated gonad tissues; s3, digesting, filtering and centrifugally purifying the resuscitated gonad tissues to obtain germline stem cells; s4, injecting morpholino to obtain gobiocypris rarus without primordial germ cells; and S5, transplanting the germline stem cells into gobiocypris rarus juvenile fish bodies, and culturing to obtain the myxocyprinus asiaticus gametes. S6, the generated myxocyprinus asiaticus gametes are fertilized, and myxocyprinus asiaticus individuals are obtained. According to the method, the sperms of the myxocyprinus asiaticus are successfully obtained by utilizing a gonad cryopreservation and abdomen borrowing reproduction technology, and a feasible way is provided for individual genetic resurgence of rare and endangered fishes.

This disclosure relates to the use of heat treatment to increase the haploid editing rate in offspring produced from haploid-induced edited lines. During hybridization, parental gametes fuse to form an embryo; and simultaneously, a gene-editing molecular system is delivered to this embryo. Applying heat treatment to the pollinated plants improves the haploid editing rate. During embryo development, one set of parental chromosomes disappears, and the gene-editing molecular system acts on the remaining set of chromosomes. Thus, hybridization produces at least one haploid offspring with the edited gene.

The invention discloses an inducible full-triploid seed production method for marine pearl shells. According to the method, the 100% triploid marine pearl oyster filial generation is obtained through the links of mature gamete acquisition, artificial sperm and egg insemination, treatment opportunity selection, treatment duration determination, full triploid culture and the like, and the purpose of creating a tetraploid production effect under the condition that no tetraploid is used as a core germplasm resource is achieved. The method provided by the invention is not influenced by environmental factors in use, and has the advantages of high stability and good repeatability. According to the method, the international blank of full triploid production of marine pearl shells is filled up, and China is promoted to reach the international leading level in the field.

The application relates to the field of homologous tetraploid organisms, in particular to a combined determination method of homologous tetraploid double-reduction parameters and HWD coefficients. The method comprises the following steps: constructing a homologous tetraploid natural population double-reduction and linkage disequilibrium combined model; constructing a combined calculation model; inputting actual values of double-marker offspring zygote frequencies into the combined calculation model to determine estimated values of double-marker direct parent gamete frequencies; calculating estimated values of single-marker direct parent gamete frequencies according to the estimated values of the double-marker direct parent gamete frequencies; inputting the estimated values of the single-marker direct parent gamete frequencies into a single-marker direct parent gamete frequency calculation model to obtain a nonlinear equation; and determining the measured values of the double-reduction parameters and the Hardy-Weinberg disequilibrium coefficients according to the nonlinear equation. The application simultaneously completes the determination of double-reduction frequency estimation, Hardy-Weinberg disequilibrium and linkage disequilibrium between markers by constructing a combined model, and improves the precision of parameter estimation.

The invention discloses shrimp and crab breeding sperm and egg automatic insemination and distribution equipment which comprises a base, a positioning block is installed at the top end of the base, inlets are formed in the top ends of a sperm collecting bin, an egg bin and a water bin, and a stirring mechanism comprises a servo motor installed on one side of a fertilization bin. The servo motor is installed on one side of the fertilization bin, the servo motor works to drive the rotating shaft to rotate, the rotating shaft rotates to drive the miniature magnetic stirrer to rotate, the servo motor is driven at a low speed, the miniature magnetic stirrer is made of a polytetrafluoroethylene coated magnetic core material, and the edge of the miniature magnetic stirrer is subjected to arc chamfering treatment; the stirring time is set to be 3-5 seconds, sperm motility decline or ovum rupture caused by too large stirring intensity is avoided, no stirring shaft is exposed in the structure, pollution dead angles are reduced, and a mature low-damage scheme for mixing the aquatic gametes is provided; therefore, the purpose that the automatic insemination and distribution equipment for the sperms and the eggs for shrimp and crab breeding is convenient for stirring gametes is achieved.

The invention provides a construction method of a male sterile line of corn, and relates to the technical field of plant breeding, the method comprises the following steps: designing double sgRNAs on two sides of a key functional region of a nuclear fertility gene of corn, and inducing exon region deletion mutation through Cas9 mediated double-site cutting; introducing the editing element into a donor plant, hybridizing with a receptor selfing line, backcrossing to a BC2 generation, and selfing to obtain a homozygous deletion mutant without the editing element; the method comprises the following steps: constructing a multi-control maintenance breeding construct containing a fertility restoring gene, a pollen inhibition module and a selection marker, enabling the multi-control maintenance breeding construct to exist in a maintainer line in a semi-zygote state, limiting the formation of male gametes containing the construct through the pollen inhibition module, and realizing automatic sorting of sterile line seeds by utilizing the selection marker. According to the scheme, complete and stable sterile characters are ensured through double sgRNA induced large fragment deletion, a non-transgenic homozygous mutant is obtained by combining a backcross selfing strategy, meanwhile, efficient and pure breeding of sterile line seeds is achieved through a multi-control maintenance system, and the hybrid seed production efficiency and the seed purity are remarkably improved.

The invention discloses an efficient preparation method of marine pearl oyster larvae. Through the technical links of gamete maturity evaluation, gonad mucus removal, ovum maturation promotion and activation, sperm motility excitation, excessive sperm fertilization and the like, the hatching rate of 90% or above is obtained, and therefore the purpose of efficient larva preparation is achieved. The method is different from the traditional method of dissecting mixed sperms and eggs for filtering, placing the sperms and eggs in a hatching container for settling for 1 hour, then pouring out supernate and directly scattering the sperms and eggs settled to the bottom into a pool for hatching, the solidification cognition of traditional marine pearl oyster breeding is subverted, the defects of low hatching rate, poor stability and the like of the traditional method are overcome, a large number of high-quality larvae are obtained, and the breeding efficiency is improved. And a technical guarantee is provided for high-efficiency production of offspring seeds, heterosexual pairing breeding and creation of excellent strains in the future. The method has the advantages of simplicity and convenience in operation, high repetition rate, high practicability and the like.

The invention provides a method for reconstructing functional gametes of fish cryopreserved germline stem cells based on zebra fish nanos2- / -mutant receptors, which specifically comprises the following steps: S1, collecting embryos of selfing progenies of zebra fish nanos2- / -and nanos + / -mutants, culturing and screening out nanos2- / -mutants to obtain receptor fish; s2, after gonads of the cryopreserved fishes are recovered, donor fish germline stem cells are obtained; s3, transplanting the germline stem cells of the body fish in the step S2 into the receptor fish in the step S1, culturing to obtain positive individuals, and continuously culturing to obtain the functional gametes of the zebra fish. Based on a selection strategy taking a nanos2 <- / -> mutant receptor as an excellent receptor, verification is carried out in experimental animal zebra fish cryopreserved germline stem cells: colonization and differentiation of germline stem cells from a donor source can be effectively supported, so that functional ova and sperms are generated, and the limitation that donor ova are difficult to obtain in an existing belly borrowing reproduction technology is broken through; and a new way is provided for long-term preservation and recovery of fish germplasm resources.

This invention relates to a male gamete protection gene S19g-A7 and its application in improving rice hybrid fertility and hybrid rice breeding. This invention identifies a male gamete protection gene S19g-A7 at the S19 locus of interspecific hybrid sterility in rice, whose encoded amino acid sequence is shown in SEQ ID NO.2. Introducing the S19g-A7 gene into Asian rice for functional complementation can break the S19 locus-mediated interspecific hybrid sterility in rice, rapidly creating hybrid compatible lines that overcome interspecific hybrid sterility in Asian and African rice. This provides genetic resources and breeding methods for utilizing distant hybrid vigor in high-quality rice germplasm breeding, and has significant application value.

The application belongs to the technical field of genetic engineering and plant genetic breeding, and particularly relates to application of a new tetraploid rice fertility gene NTFR2. The application uses a gene knockout technology to determine the function of the fertility regulation gene NTFR2 in the new tetraploid rice, and finds that the NTFR2 affects the fertility of the new tetraploid rice. When the function of the NTFR2 gene is lost, the seed setting rate of the mutant is reduced, and the development of the female gametophyte sac exhibits abnormality. Meanwhile, the loss of the function of the NTFR2 gene leads to a significant decrease in the fertility of the new tetraploid rice, but has a smaller influence on the male gametes and other agronomic traits, which indicates that the NTFR2 is a new gene mainly affecting the fertility of the tetraploid rice, and has an important application prospect in the fields of creating a new female sterile line and further promoting the utilization of hybrid vigor of the tetraploid rice.

The application provides a method for genetic reactivation of individual Procyprinus palpebrosus, comprising the following steps: S1, taking the gonadal tissue of Procyprinus palpebrosus, washing, cutting, adding L-15 culture medium containing double antibodies and DPBS buffer solution to centrifuge the gonadal tissue; S2, water-bathing the gonadal tissue to obtain recovered gonadal tissue; S3, digesting the recovered gonadal tissue, filtering, centrifuging and purifying to obtain germ stem cells; S4, injecting morpholino to obtain rare gobi-ocypris rarus with deleted primordial germ cells; S5, transplanting the germ stem cells into the body of the rare gobi-ocypris rarus juvenile to culture, and obtaining the gametes of Procyprinus palpebrosus; and S6, fertilizing the generated gametes of Procyprinus palpebrosus to obtain the individual Procyprinus palpebrosus. The application successfully obtains the sperm of Procyprinus palpebrosus by using the cryopreserved gonadal tissue and the technology of parthenogenesis, and provides a feasible way for the individual genetic reactivation of rare and endangered fish species.

The present invention relates to an open, retrievable intrauterine device comprising an adhesive means, and in particular for culturing gametes and / or embryos in the uterus.

Disclosed are methods for selecting or identifying fusion-competent gametes. The methods can include providing a sample including gametes obtained from a subject; and contacting the sample with a reagent capable of binding Myomerger expressed on the surface of the gametes. The methods can also include isolating gametes bound by the reagent capable of binding Myomerger from gametes not bound by the reagent capable of binding Myomerger. Also disclosed are methods for contraception that include administering a reagent capable of binding Myomerger expressed on the surface of gametes to a subject. Also disclosed are compositions that include a reagent capable of binding Myomerger and a reagent capable of binding phosphatidylserine, as are kits that include the disclosed compositions.

The invention belongs to the technical field of genetic breeding of aquatic animals, and particularly relates to a method for producing male gametes of finless eels based on a germline stem cell transplantation technology. The method comprises the following steps: firstly, obtaining a cell suspension from gonads of one-year-old female ricefield eel, centrifuging to obtain germline stem cells, adding a fluorescent label, injecting the germline stem cells into bodies of receptor fish two-year-old male mastacembelus armatus, checking testis of the mastacembelus armatus by using a fluorescence microscope, selecting the receptor mastacembelus armatus subjected to colonization differentiation of the germline stem cells of the ricefield eel, breeding to a breeding season the male gametes for artificial insemination of the ricefield eels are obtained. According to the method for special germ cell allograft and functional gamete generation of ricefield eel, donor-sourced gamete can be generated in a short time, the problems of long ricefield eel breeding period, breeding difficulty and the like are solved, and the method has important significance on ricefield eel research and large-scale breeding.

A method, as can be applied, for example, in in vivo reproduction and in healthcare, and to a device for realizing the controlled movement. The device can be used in a considerably less invasive manner and significantly higher implantation rates can be realized using the method. By the method, gametes and / or zygotes and / or embryos are releasably connected to one or more carriers, and the carriers, with or without gametes and / or zygotes and / or embryos, are moved in a directed manner autonomously or by external influences. The device includes at least one carrier having maximum dimensions in all spatial directions of 500 μm, which carrier is composed at least primarily of biocompatible materials.

1. The name of the design product: embryo incubator (DCK6). 2. The use of the design product: used for the culture of gametes, embryos and other cells in assisted reproductive technology (ART). 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: design 1 perspective view 1. 5. Design 2 rear view is the same as design 1 rear view, omit design 2 rear view; Design 2 left view is the same as design 1 left view, omit design 2 left view; Design 2 right view is the same as design 1 right view, omit design 2 right view; Design 2 top view is the same as design 1 top view, omit design 2 top view; Design 2 bottom view is the same as design 1 bottom view, omit design 2 bottom view; Design 3 rear view is the same as design 1 rear view, omit design 3 rear view; Design 3 left view is the same as design 1 left view, omit design 3 left view; Design 3 right view is the same as design 1 right view, omit design 3 right view; Design 3 top view is the same as design 1 top view, omit design 3 top view; Design 3 bottom view is the same as design 1 bottom view, omit design 3 bottom view. 6. Design 1 is designated as the basic design.

The invention encompasses a method of estimating a distribution or a standard deviation of breeding values or predicted transmitting abilities of gametes from an individual using population-wide estimates of linkage disequilibrium for one or more genetic markers or genes, so as to more efficiently produce offspring having breeding values or predicted transmitting abilities that equal or exceed a desired breeding value or predicted transmitting ability.

This invention relates to a method for artificial hybridization breeding of male brown-spotted grouper and female humpback bass. The method belongs to the field of distant hybridization breeding technology. It includes the selection of brown-spotted grouper and humpback bass parents, parent maturation cultivation, gamete collection, artificial insemination and hatching, and seedling cultivation. This invention uses reproductive regulation and distant hybridization technology to hybridize two species belonging to two genera within the grouper family with different geographical distributions and different breeding times, that is, intergeneric hybridization within the grouper family, which breaks through the reproductive isolation between genera, thereby cultivating superior varieties with fast growth and high survival rates.