98 results about "Gamete" patented technology

The invention relates to an artificial hybridization fry breeding method for epinephelus fuscoguttatus and pink pink bass, which belongs to the technical field of distant hybridization breeding and comprises the steps of parent selection of epinephelus fuscoguttatus and pink pink bass, parent breeding, parent intensive breeding, gamete collection, artificial insemination, hatching of fertilized eggs and fry breeding. By means of reproductive regulation and distant hybridization technologies, two species belonging to two genera under the grouper family with different geographical distribution and different breeding time are hybridized, namely cross-generic hybridization under the grouper family is achieved, intergeneric reproductive isolation is broken through, and the breeding efficiency is improved. Therefore, new hybridized germplasm with excellent characteristics of high fertilization rate, high hatching rate, high survival rate, high growth speed and the like is cultivated.

The invention relates to the technical field of microorganisms, in particular to application of butyric acid producing enterobacter as a probiotic for resisting eimeria tenella. In the invention, an antibiotic cocktail-induced eimeria tenella infection model is utilized to explore the effect of butyric acid-producing enterobacter DSM 26588 on eimeria tenella development. The enterobacter butyricum DSM 26588 inhibits the growth of gametes by inhibiting the expression of the eimeria tenella gamete EtGFAT gene; according to the present invention, the intestinal tract lesion caused by Eimeria tenella infection can be improved, the intestinal tract pathological injury can be alleviated so as to reduce the Eimeria tenella oocyst discharge amount, the ACI value is 162, and the good anti-coccidiosis effect can be provided.

The invention provides an intelligent gamete and embryo storage management system for a reproductive center, which comprises a heat preservation module, a liquid nitrogen storage module, a liquid nitrogen storage module, a liquid nitrogen storage module and a liquid nitrogen storage module which are connected with one another, the liquid nitrogen recycling module is used for recycling, separating and purifying gaseous nitrogen by utilizing a condenser and a closed-loop pipeline; the intelligent monitoring module is used for monitoring the liquid nitrogen tank in real time by using various sensors, processing and analyzing monitoring data, and calculating and judging whether the current state is a safe state or not; the analysis and prediction module is used for calculating the fault probability of the liquid nitrogen tank by utilizing a machine learning model; according to the high-density storage module, a multi-layer storage frame, a single-tube container and a multi-tube container are installed in the liquid nitrogen tank; and the centralized management module is used for constructing a sample file and remotely monitoring the real-time state and abnormity of the liquid nitrogen tank. Liquid nitrogen volatilization can be reduced to the maximum extent, the sample safety is guaranteed, the storage efficiency is improved, and the increasing requirements of test tube babies are met.

The invention discloses a method for separating, purifying and transplanting spermatogonial stem cells of lateolabrax japonicus, the spermatogonial stem cells of lateolabrax japonicus are separated and purified, marine fish larvae or freshwater fish larvae are used as receptor larvae, and then the spermatogonial stem cells of lateolabrax japonicus are transplanted to abdominal cavities of the receptor larvae. The spermatogonial stem cells obtained through separation are complete in number, structure and function and low in cell clustering rate. After the transplanted spermatogonial stem cells are subjected to fluorescence labeling, the migration and proliferation processes of donor germ cells can be observed in receptor fishes of marine fish fries or freshwater fish fries, so that the receptor fishes with the chimeric germline are obtained. According to the method, through xenotransplantation of the spermatogonial stem cells, a'belly borrowing reproduction 'breeding mode is provided for the marine fishes, a new way for gamete preservation and rapid propagation is provided for the marine fishes with the long reproductive cycle, and a new method and a new thought are provided for genetic improvement and germplasm resource preservation.

The invention relates to a male gamete killer gene S19g-A1 of a rice interspecific hybrid sterile S19 seat and application of the male gamete killer gene S19g-A1. The invention provides a rice hybrid sterility related protein S19g-A1. The amino acid sequence of the rice hybrid sterility related protein S19g-A1 is as shown in SEQ ID NO. 2. According to the invention, a key gene S19g-A1 of a rice interspecific hybrid sterile S19 site is researched and identified, function knockout is carried out on African rice S19g-A1, and pollen fertility of a mutant hybrid F1 obtained by carrying out distant hybridization on an obtained African rice s19g-a1 mutant returns to normal. Based on a gene editing technology, the invention develops a method for rapidly creating a hybrid affinity line capable of overcoming sterility of African rice hybrid, effectively eliminates S19 locus mediated interspecific hybrid reproductive disorder, breaks through the bottleneck of rice interspecific hybrid advantage utilization, provides target gene resources for distant hybrid advantage utilization and crop yield improvement, and has a wide application prospect. Good application prospects are realized.

The invention relates to an artificial cross breeding method for male epinephelus fuscoguttatus and female humpback perch, which belongs to the technical field of distant cross breeding and comprises the steps of parent selection of the epinephelus fuscoguttatus and the humpback perch, parent ripening cultivation, gamete collection, artificial insemination and incubation and fry cultivation. By means of reproductive regulation and distant hybridization technologies, two species of two genera of grouper family in different geographical distribution and different reproduction time are hybridized, namely cross-generic hybridization of grouper family breaks through the generic reproductive isolation, and therefore good varieties which grow fast and are high in survival rate are cultivated.

The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.

The invention relates to the technical field of livestock and poultry breeding, in particular to a goat hybridization breeding method capable of achieving complete-cycle nutrition enhancement. The core of the method is that systematic nutrition intervention runs through the whole breeding process. Before hybridization, performing differential nutrition pretreatment on the parents for 60 days: feeding the female parents with a breeding nutrition enhancer added with components such as D-chiro-inositol and N-acetylcysteine, and supplementing sperm motility enhancer containing acetylated L-carnitine and ergothioneine to the male parents. In the gamete treatment stage, the oocytes are subjected to in-vitro maturation culture in a special culture solution containing components such as D-chiro-inositol and a growth differentiation factor 9; after the sperms are subjected to gradient centrifugal screening, completing in-vitro fertilization in an optimized fertilization culture solution; after the embryos develop into blastocysts in the sequential culture system, carrying out laparoscopic transplantation, and carrying out staged precise nutrition culture on the pregnant ewes. According to the method, through multi-level nutrition enhancement, the reproductive capacity of the goats and the growth performance of offspring are effectively improved.

The invention discloses a system for directionally differentiating chicken primordial germ cells into gametes in vitro and a regulation and control method, the system comprises a basic culture medium: based on DMEM / F12, adding 10% of fetal calf serum, 2% of chicken serum, 2 mmol / L of L-glutamine, 1 mmol / L of sodium pyruvate and 5.5 * 10 <-5 > mol / L of beta-mercaptoethanol; the spermatogonium induction culture medium further comprises BMP4, RA and GDNF; the oogonium induction culture medium also comprises BMP15, GDF9 and EGF (Epidermal Growth Factor). According to the system and the regulation and control method, the differentiation efficiency is improved, the culture period is shortened, and the number of cell clusters is remarkably increased.

The present inventions relate to a breeding system for the production of polyploid maize seeds, maize plants, or maize plant parts where cycles of meiosis, syngamy, and selection are used for interpopulation improvement of progenitor lines, and sexual polyploidization occurs during hybrid production by inducing clonal gamete formation in the parents that are to be crossed. Reciprocal recurrent selection can be used to inform selection of candidate maize lines that are either advanced to a gene editing or genetic modification system or crossed and selected to induce clonal gamete formation by arresting meiotic recombination and chromosome reduction. Crosses of parent maize plants bearing clonal gametes are planned and executed based upon predicted heterotic performance at the polyploid level. The final product is a homogeneous population of hybrid polyploid maize seed, or derivative thereof, bearing both parents' complete nuclear genomes.

Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing embryogenesis factor genes and / or morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing embryogenesis factor gene products and / or morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Embryogenesis factor proteins and / or developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

The present invention provides a method for detecting single-cell gene sequence variation in rice and its application, which belongs to the field of agricultural technology. The method of the present invention mainly includes three aspects: sampling, sequencing and bioinformatics analysis, wherein the sampling is to obtain a single rice microspore sex cell. The present invention successfully isolates a single rice microspore sex cell, and improves the defects of the existing rice single-cell variation detection technology process, such as weak integrity and detection consistency that need to be improved. In addition, the authenticity of the mutation site is identified and confirmed by heavy ion mutagenesis technology. The method of the present invention broadens the source range of rice single-cell mutagenic materials, and provides new ideas and methods for creating more diverse breeding materials and conducting more efficient mutagenic breeding. At the same time, microspore sex cells serve as male gametes, and the mutations carried in the genome can be passed into the offspring through hybridization, which can realize the inheritance and fixation of mutations, and also provide a way to quickly utilize excellent mutant genes.

Reserves of immortalized genetic material are stored in a bank for providing a resource (e.g., artificial gametes) for couples (e.g., same sex couples) to produce biologically-related children. The reserves provide the ability to derive sperm from induced pluripotent stem cells (iPSC's) of one partner and / or eggs from iPSC's of the other partner. For example, a biological sample is stored as iPSC's that can be used to generate an unlimited supply of genetic material (e.g., artificial gametes for conception) when needed by a user (e.g., a year or more after the sample is provided, e.g., 5 years or more after the sample is provided). Such a bank is helpful for an individual in a same sex (or infertile) couple who desires to have biological children at some point during his / her lifetime, but does not plan to have children in the immediate timeframe, for example.

The invention discloses a soybean alpha-amylase gene-based pollen lethal module and application thereof, the soybean alpha-amylase gene-based pollen lethal module comprises a pollen specific promoter, a plastid leader peptide gene and a soybean alpha-amylase gene, and the pollen specific promoter, the plastid leader peptide gene and the soybean alpha-amylase gene are sequentially connected in a direction from 5'to 3 ', the plastid leader peptide gene and the soybean alpha-amylase gene form a fusion gene, the pollen specific promoter is a soybean GmPG031 gene promoter, the nucleotide sequence of the pollen specific promoter is as shown in SEQ ID NO: 1, the soybean alpha-amylase gene is driven by the pollen specific promoter to be directionally expressed in a male gamete, and the plastid leader peptide gene is combined to guide zymoprotein to amyloid plastid, so that the soybean alpha-amylase gene is obtained. Starch reserve in the pollen is specifically consumed, so that the pollen carrying the module is aborted due to energy failure, the mechanism realizes accurate death of the transgenic pollen, a basic element is provided for a soybean seed production technology, and a core tool is provided for large-scale creation of a male sterile line.

Methods and compositions used to prevent or decrease reduction, or reduction and recombination, during meiosis in female sporocyte, female gametophytes, or female gametes are described herein. The compositions and methods may include silencing elements and / or use gene-editing technology to decrease or inhibit Nrf4 expression levels or activity. Further provided are compositions and methods for the production of a plant that has ovules comprising non-reduced, or non-reduced and non-recombined, gametes, and / or seeds that contain clonal maternal embryos.

The invention relates to the use of carbon monoxide for preserving gametes. Means and methods for preserving gametes are provided. The method of the invention comprises the steps of a) providing a gamete or a sample comprising said gamete in a container, and b) contacting said gamete in said container with carbon monoxide. The invention also provides the use of carbon monoxide in the treatment and / or prevention of diseases, such as diseases caused by or associated with elevated DNA cleavage, redox potential of gametes, and / or diseases of gametes caused by or associated with elevated ROS levels. Also provided are uses of carbon monoxide for the prevention of congenital abnormalities and / or aneuploidy, as well as for the treatment of diseases caused by and / or associated with elevated DNA cleavage and / or redox potential of gametes. The invention also relates to a method of treating congenital abnormalities or aneuploidy comprising contacting carbon monoxide gas with gametes of a patient in need thereof. Thus, the means, methods and uses of the present invention ensure that gametes are preserved in a functional / intact state, thereby ensuring maintained and / or improved gamete quality. Thus, such carbon monoxide treated / carbon monoxide exposed gametes are particularly useful in reproductive techniques, such as in vitro reproductive techniques, including in human assisted reproductive techniques and / or in animal (artificial) insemination techniques.

The invention relates to the technical field of fish cultivation, in particular to a method for cultivating all-male tetraploid crucian carp, which comprises the following steps of: performing artificial spawning induction by taking gynogenesis homologous diploid crucian carp as a female parent and taking androgenesis homologous diploid crucian carp as a male parent; wherein the sex chromosome type of the gynogenesis homologous diploid crucian carp is XX and generates non-reduced diploid ovum, and the sex chromosome type of a male individual in the androgenesis homologous diploid crucian carp is XY or YY and generates non-reduced diploid sperm; artificial insemination is carried out, obtained fertilized eggs are hatched and cultured to sexual maturity, and survival offspring is the all-male tetraploid crucian carp population. Based on the characteristic that gynogenesis homologous diploid carassius auratus and androgenesis homologous diploid carassius auratus both generate non-reducing gametes, the gynogenesis homologous diploid carassius auratus and the androgenesis homologous diploid carassius auratus are subjected to artificial insemination, and all-male tetraploid The all-male tetraploid crucian carp can reach a sexual maturity state after being only 120 days old, diploid sperms can be stably generated, and the reproductive performance is improved.

The application discloses a method for creating polyploid fish, which comprises the following steps of: knocking out a spo11 gene by using a CRISPR / Cas9 gene knockout technology, obtaining a spo11 homozygous knockout individual through genotyping and breeding, utilizing the characteristic that a spo11 homozygous knockout diploid female can produce part of unmeiotic oocytes to obtain a triploid, and further screening a spo11 homozygous knockout triploid 3N spo11 ‑ / ‑ / ‑ ; utilizing the characteristic that a 3N spo11 ‑ / ‑ / ‑ female can produce part of unmeiotic oocytes to further screen a heterozygous knockout tetraploid 4N spo11 + / ‑ / ‑ / ‑ ; utilizing the characteristic that a 4N spo11 + / ‑ / ‑ / ‑ can produce a large number of diploid gametes to further prepare a large number of triploid offspring. Compared with other polyploid breeding methods, the application has universality and genetic controllability, and provides important technical support for artificially inducing fish polyploidy.

This patent discloses a breeding method for selecting near-isogenic wheat lines using the isoarm chromosome i4JL of *Thinopyrum besarmentosum*. The i4JL chromosome has a unique fluorescent band, making it easily distinguishable from wheat chromosomes. In a wheat background, it is primarily transmitted through female gametes, with extremely low transmission rates in male gametes. Furthermore, it contains two doses of the blue grain gene. Therefore, the self-crossing progeny of the wheat-*Thinopyrum besarmentosum* i4JL monosomatic addition line MAi4JL always exhibits easily separable blue and non-blue grains. By crossing MAi4JL with any non-blue grain wheat and selecting blue grain seeds from the hybrid progeny for five consecutive generations, near-isogenic wheat lines with different backgrounds and phenotypes (blue and non-blue grains) can be obtained, providing a new method for selecting near-isogenic wheat lines. Since i4JL significantly reduces plant height in different wheat backgrounds and significantly affects gene expression in wheat stems and grains, it has significant application potential in wheat genetic breeding research and applications.

A method for efficiently inducing genomic variation in rice microspore sexual cells, relating to the technical field of plant cell engineering. In the method, a rice male gamete (early uninucleate microspore) is used as a mutagenic material, it is attempted, for the first time, to irradiate rice microspore sexual cells with heavy ions (4 Gy) and γ rays (10 Gy), experimental technologies such as fluorescence microscope observation, FDA-PI dyeing, and immunofluorescence detection are taken into account to determine three indicators comprising a target period for irradiation sample sampling, an appropriate heavy ion / γ ray radiation dose, and an appropriate sampling time after the end of radiation, and a relatively complete rice microspore sexual cell mutagenesis system is established. The method involves clear indicators, strong operability, can support batch mutagenesis operations, and can provide a technical system support for the efficient batch production of rice sexual cell mutants.

The invention relates to the technical field of cyclina sinensis sperm preservation, in particular to a cyclina sinensis sperm freezing preservation method. The cyclina sinensis sperm freezing preservation method comprises the following steps: (1) putting parent shellfish into a seawater culture pond, and accelerating ripening for 7-10 days by using a ripening agent; the ripening agent is prepared from a spirulina extract, a radix paeoniae alba extract, a codonopsis pilosula extract, astaxanthin and alginic acid; (2) controlling 0.05-0.15 m / s water flow to scour and stimulate the cyclina sinensis, selecting individuals with sperms discharged after the parent shellfish gametes start to be discharged, and collecting the sperms of the cyclina sinensis; and (3) mixing the cyclina sinensis sperms with a cryoprotectant, pre-cooling, and then cryopreserving for a long time. Research results show that the use of the cryoprotectant can significantly reduce the degree of DNA damage, and the protection effect may be realized through a plurality of mechanisms such as direct combination of trehalose with DNA molecules, free radical removal, maintenance of the stability of a chromatin structure and the like.

The invention relates to a three-way hybrid grouper new germplasm cultivation method, and belongs to the technical field of grouper genetic breeding. According to the method, epinephelus moara and golden tiger hybridized fries (female epinephelus fuscoguttatus * male epinephelus coioides) are used as breeding materials, three-way hybridization is carried out in a matching mode of female epinephelus moara * male epinephelus coioides, and breeding of three epinephelus genetic species and fry with excellent characteristics is achieved. The method comprises the steps of parent selection, parent fish reproduction regulation and control, parent fish spawning induction, gamete collection, artificial insemination, incubation and fry rearing. According to the method, on the basis of reproductive regulation and crossbreeding technologies, time and space reproductive isolation of the groupers is broken through, and the new grouper germplasm which is high in survival rate, low in malformation rate and high in growth speed is cultivated.

The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross.

The present application relates to fish breeding technology field, especially to a kind of all-male tetraploid crucian carp breeding method, comprising the following steps: with gynogenesis homologous diploid crucian carp as female parent, and androgenesis homologous diploid crucian carp as male parent, artificial delivery is carried out;Wherein, the gynogenesis homologous diploid crucian carp sex chromosome type is XX, produces non-reduction diploid egg, and the androgenesis homologous diploid crucian carp male individual sex chromosome type is XY or YY, produces non-reduction diploid sperm;Artificial insemination is carried out, and the obtained zygote is hatched, and cultured to sexual maturity, and the surviving offspring is all-male tetraploid crucian carp population.The present application is based on the characteristics that gynogenesis homologous diploid crucian carp and androgenesis homologous diploid crucian carp both produce non-reduction gametes, artificial insemination is carried out, and all-male tetraploid crucian carp can be prepared on a large scale.The all-male tetraploid crucian carp only needs 120 days of age to reach sexual maturity, can stably produce diploid sperm, and the reproductive performance is improved.

The invention provides a plant male gamete fertility control method and a method for obtaining a male sterile material, and belongs to the technical field of plant breeding. According to the invention, the PPCD1 is taken as a target gene, and the PPCD1 gene is subjected to expression inhibition or gene knockout, so that the PPCD1 gene loses functions, pollen development fails, and the purpose of fertility control is achieved. Therefore, the male sterility material can be created through the method, and the method can be used for creating male sterility materials in cross breeding, creating materials for preventing genetic escape of transgenic plants, creating plant materials without pollen pollution and the like, so that the production efficiency, the economic effect and the social and environmental effects are greatly improved.

This invention discloses a method for the isolation, culture, and identification of chicken sexual gonadal cells, belonging to the field of biotechnology. The invention selects chicken embryos hatched to 18.5 days old, isolates the gonads after PCR sex identification, and uses 0.25% trypsin-EDTA digestion followed by filtration through a 70μm filter to obtain a single-cell suspension with a survival rate >90% and free from PGC contamination. A sex-specific culture system is established, allowing cells to be stably passaged and maintain a sex-specific phenotype. Through morphological, qRT-PCR, immunofluorescence, and Western blot multidimensional identification, accurate identification of male testicular Sertoli cells and female ovarian granulosa cells is achieved. This method is simple to operate, has good reproducibility, and produces high cell viability and purity. It can provide a stable gonadal microenvironment for the in vitro directed differentiation of chicken primordial germ cells, significantly improving gamete induction efficiency. It is suitable for research on avian reproductive and developmental mechanisms, transgenic breeding, and germplasm resource preservation.

The invention relates to a construction method and application of a tilapia mossambica meiosis recombination model based on rec8b gene editing, and belongs to the field of gene engineering. The method aims at solving the technical problems that in existing animal meiosis model construction, a physical / chemical induction method is low in polyploidy rate and cannot be inherited, and a gene editing method is accompanied by cell apoptosis and cannot obtain fertile gametes. The invention provides a construction method of a tilapia meiosis recombination model based on rec8b gene editing, which comprises the following steps: designing sgRNA of a targeted rec8b gene exon3, mixing the sgRNA with Cas9mRNA according to a ratio, then microinjecting the mixture into a single cell stage fertilized egg, and carrying out F0 generation backcross and F2 generation screening to obtain a homozygous mutant as a model. The genetic and fertile vertebrate meiosis recombinant living body model is established, and an industrial and scientific research technology platform is provided for meiosis mechanism research and polyploid breeding.

1. The name of the design product: the storage and identification device of sperm and ovum in the reproductive laboratory. 2. The use of the design product: the storage and identification of the test tube containing sperm and ovum in the reproductive laboratory. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best shows the design points: perspective view.

This invention discloses a simple and rapid breeding method for artificially synthesizing wheat. It involves inducing incomplete meiosis in *Gnaphalium affine*, a close relative of wheat, under high red light (R / FR≥1.4) to produce unmeiotic divisions. This method falls under the field of crop breeding technology and includes the following steps: *Gnaphalium affine* (DD) cultured in a high red light (R / FR≥1.4) environment undergoes incomplete meiosis during reproductive growth to produce unmeiotic gametes. These gametes are then used as the male parent and crossed with *Langdon* (AABB). The resulting F1 generation (ABDD) is tetraploid. The F2 generation obtained by self-pollination of this F1 generation is hexaploid (AABBDD). Furthermore, the artificially synthesized hexaploid wheat offspring can be stably inherited.

The present invention discloses a method for artificial breeding of tropical sea cucumbers. The method comprises the collection of sea cucumber parents, acquisition of gametes, fertilization and hatching, cultivation of planktonic larvae, attachment and cultivation of larvae, and intermediate culture of tropical sea cucumbers. For the first time, sea cucumbers of the jade foot, sea cucumbers of the brown ring, and sea cucumbers of the mikkelsen are bred by dissecting and obtaining sperm and eggs. The method provides a technical guarantee for the continuation and resource recovery of tropical sea cucumber populations that are on the verge of extinction and cannot undergo artificial induction of spawning. The method is simple and easy to operate for artificial breeding of tropical sea cucumbers, and can effectively overcome a series of problems in the seedling cultivation process, such as the inability to induce spawning / poor induction of spawning effect, unstable output, and unscientific feeding of baits. The success rate of artificial breeding of tropical sea cucumber seedlings is improved. The method does not damage key structures such as the body wall and other organs of the parent organism, and has a very broad application space in the field of artificial breeding and aquaculture of tropical sea cucumbers.