16 results about "Sperm sorting" patented technology

Systems for sorting sperm include a housing and a microfluidic system supported by the housing. The systems also include two or more inlets providing access to the microfluidic system to deliver sperm or semen and fluid to the microfluidic system, as well as an outlet for harvesting sorted sperm. The systems include a flow channel that provides a flow path for sperm from an inlet to an outlet while sperm travels against a fluid flow towards an outlet for harvesting. In the systems, fluid delivered to the microfluidic system via an inlet flows from the inlet towards one or more outlets, and the flow channel extending along the length of the microfluidic system provides a flow path for motile sperm to travel against the fluid flow towards a collection outlet for harvesting. In some embodiments of a system, the microfluidic system also includes a microporous filter arranged in the flow path between the inlet and the collection outlet to cause motile sperm traveling against the fluid flow to pass through the filter to reach the collection outlet for harvesting.

To provide a sperm selection aid that enables the selection of good quality sperm suitable for intracytoplasmic sperm injection (ICSI) from among the sperm in a sample. [Solution] A sperm sorting assistance device comprising: an index calculation unit that calculates at least one index related to the quality of sperm recognized in a video; an evaluation unit that relatively evaluates the quality of first sperm recognized in a first time interval in a video and second sperm recognized in a second time interval different from the first time interval in a video, based on at least one index; and a display control unit that displays information relating to at least one index of the first sperm to the first sperm in the first time interval in a video, and displays the relative quality evaluation of the first sperm and the second sperm evaluated by the evaluation unit.

This makes it possible to select high-quality sperm suitable for intracytoplasmic sperm injection (ICSI) from among the sperm in a sample. To provide a sperm sorting assistance device. [Solution] Calculate at least one indicator related to sperm quality recognized in the video. An index calculation unit and, based on at least one index, during a first time interval in the video The first sperm recognized, and in a second time interval different from the first time interval in the video image An evaluation unit that relatively evaluates the quality of the second sperm recognized by the first time in the video During the interval, information regarding at least one indicator of the first sperm for the first sperm. The relationship between the first sperm and the second sperm, as evaluated by the evaluation unit, is displayed. A sperm sorting assistance device comprising a display control unit that displays an evaluation of relative quality.

An automated sperm sorting device, comprising an original semen tray, a chip tray, a heat insulation block, two horizontal slide rails, two longitudinal slide rails, a connecting rod, a plurality of suction devices, and a controller. The original semen tray comprises a first tray body and a first temperature controller. The first temperature controller is controlled by the controller to provide a first temperature to a first upper surface of the first tray body. The chip tray is provided on one side of the original semen tray, and comprises a second tray body, a sorting set, and a second temperature controller. The sorting set is provided in a chip slot of the second tray body. The second temperature controller is controlled by the controller to provide a second temperature to a second upper surface of the second tray body. The heat insulation block is provided between the first tray body and the second tray body. By means of the two horizontal slide rails and the two longitudinal slide rails, the controller controls the connecting rod so as to move the plurality of suction devices arranged on the connecting rod to different positions.

This invention discloses a porcine sperm-specific antibody, the coding sequence of which is shown in any one of SEQ ID No: 1-3. This invention prepares a nanobody library by immunizing alpacas with whole porcine sperm. The library can then be screened to identify antibodies that specifically bind to X or Y sperm, thus achieving X and Y sperm sorting. Furthermore, the screened antibodies are nanobodies, only 10% the size of traditional antibodies, with a simpler structure, high stability, strong specificity, and low immunogenicity. This allows for large-scale antibody expression, significantly reducing antibody production costs and facilitating industrial application.

The invention relates to the technical field of sperm sorting, in particular to a goat X and Y sperm sorting solution, a sorting reagent and a sorting method. The X and Y sperm sorting liquid takes water as a solvent, and comprises the following components by concentration: 24-27 g / L of glucose, 15-18 g / L of citric acid, 14-17 g / L of sodium ethylene diamine tetracetate, 55-59 g / L of sodium chloride, 19-22 g / L of sodium bicarbonate, 32-35 g / L of potassium chloride, 5.6-6.0 g / L of Tris, 0.4-0.8 g / L of BSA and 1.0 [mu] mol / L of requinimod R848. The R848 regulates and controls the movement performance of the goat X sperms, so that not only is the molecular effect of the R848 on an interaction network and phosphorylation modification of X sperm metabolites clarified, but also a brand new thought and theoretical basis are provided for precise optimization and safety evaluation of a sperm sorting technology.

The present disclosure provides a sperm sorting assistance device that enables selection of high-quality sperm suitable for intracytoplasmic sperm injection from among sperm in a specimen. The present disclosure provides a sperm sorting assistance device comprising: an index calculation unit that calculates at least one index relating to the quality of sperm recognized in a moving image; an evaluation unit that, on the basis of the at least one index, relatively evaluates the quality of first sperm recognized during a first time interval in the moving image and second sperm recognized during a second time interval different from the first time interval in the moving image; and a display control unit that, in the first time interval in the moving image, displays information relating to the at least one index of the first sperm in association with the first sperm, and displays an evaluation of the relative quality of the first sperm and the second sperm evaluated by the evaluation unit.

This invention relates to microfluidic devices, and more particularly to a sperm sorting device and a sperm guiding device. A sperm sorting device is provided, comprising: an inlet chamber, an outlet chamber located radially inside the inlet chamber, and a channel connecting the inlet and outlet chambers. The channel includes blind branches with a radius of curvature between 50 μm and 75 μm. A sperm guiding device is also provided, comprising an inlet opening and an outlet opening, and blind branches with a radius of curvature between 50 μm and 75 μm. The technical advantage of this invention is the reduction of sperm backflow.

The present invention relates to the technical field of sperm sorting, and provides a sperm processing chip and a use method therefor. The sperm processing chip comprises: a second tank arranged in a first tank, wherein a sample adding pool for placement of a sperm sample to be tested is formed between the first tank and the second tank, an outer wall surface of the second tank faces the sample adding pool, the top of the outer wall surface is constructed as a slope, one end of the slope points to the sample adding pool and the other end is connected to the interior of the second tank, the slope is provided with a microchannel extending in an inclined direction of the slope, an inner wall surface of the second tank forms a collection pool for collecting sperm that flows from the sample adding pool to the microchannel of the slope and falls into the collection pool, and the tank wall height of the first tank is higher than the tank wall height of the second tank. The sperm processing chip can achieve active sorting without the use of chemicals, and sperm screening under the action of chemicals, can effectively maintain sperm vitality, and has the advantages of high versatility, high recovery rate, high sorting accuracy, high motility rate, and ease of operation.

A sperm sorting device is suitable for sorting a plurality of motile sperm from a semen sample, and includes a base and a first sorter positioned on the base. The base includes a receiving space defined by a bottom wall and a surrounding wall. The first sorter includes a first kit and a first permeable membrane. The first kit has a first sleeve for being placed into the receiving space. The first sleeve has a first inner sleeve surface and a first outer sleeve surface. The first permeable membrane is fixed to the first sleeve. The first permeable membrane and the inner wall surface of the bottom wall and the surrounding wall define a first sample receiving chamber for receiving the semen sample. In this way, the motile sperm can naturally swim upward and pass through the first permeable membrane, and the quality of sperm sorting can be improved.

The application relates to a sperm sorting device, which comprises a screening unit, a guiding unit and a storage unit connected in sequence from bottom to top, the screening unit is internally provided with microchannels and an annular channel, the microchannels are distributed radially with the axis of the screening unit as the center, the ends of the microchannels close to the axis of the screening unit meet each other and form a meeting area, the ends of the microchannels away from the axis of the screening unit are communicated with the annular channel, the screening unit is provided with an injection port communicated with the annular channel, the guiding unit is provided with a guiding cavity, and the storage unit is provided with a storage cavity. Sperms enter from the injection port, high-activity sperms in linear motion can quickly pass through the microchannels to enter the meeting area, preliminary sorting is realized, the sperms entering the meeting area move into the storage cavity in the upper direction under the guiding action of the guiding cavity, high-activity sperms can be further screened out, and finally the sperms screened out are stored in the storage cavity. Strongly active and high-quality sperms can be quickly and accurately screened out, and the working efficiency is improved.

The invention discloses a diluent for sorting pig Y sperms as well as a preparation method and application of the diluent. The diluent is prepared from the following components: 40 g / L of glucose, 0.5 g / L of sodium citrate, 0.1 g / L of creatine monohydrate, 0.75 g / L of potassium chloride, 0.0015 g / L of rotenone, 2.38 g / L of HEPES, 0.2 g / L of magnesium chloride and 1.0 g / L of bovine serum albumin. The diluent can amplify the difference of linear movement speed between X and Y sperms of pigs by regulating ATP supply, and can separate the Y sperms with higher movement speed in combination with an upstream sorting method, and the separation efficiency reaches 70-80%. The diluent disclosed by the invention has the characteristics of simplicity, rapidness, high efficiency and low cost in Y sperm separation, is almost harmless to sperms, and is suitable for being applied to boar production.

The invention relates to a yak X / Y sperm separation and enrichment method based on pH value regulation and a kit thereof, and belongs to the technical field of animal breeding and sex control. The method comprises the following steps: using an Optidyl diluent as a sperm sorting protection solution, adjusting the pH value of the Optidyl diluent through NaOH or HCl, enriching X sperms in a pH range of 6.2-6.4, and enriching Y sperms in a pH range of 6.8-7.0; the method comprises the following specific steps: diluting yak sperm, centrifugally desizing, re-suspending by using a sorting solution with corresponding pH, standing and incubating for 45-90 minutes at 35-40 DEG C and 45 DEG C, realizing natural layering according to the motility difference of X and Y sperms under different pH conditions, respectively collecting upper or lower sperms, and washing and re-suspending to obtain a sex-controllable sperm sample; the method is easy and convenient to operate, low in cost, small in sperm damage, complete in sperm structure and stable in function after separation, suitable for breeding technologies such as artificial insemination and in-vitro fertilization, capable of meeting the requirement for sexual control sperm in yak production and beneficial for optimizing the population structure and improving the breeding efficiency.

The invention relates to the technical field of animal breeding, and discloses a yak X / Y sperm separation and enrichment method based on a TLR7 / 8 agonist R848 and a kit. The method comprises the following steps: treating the yak sperm by using a sperm sorting solution containing a TLR7 / 8 dual agonist R848, incubating under specific conditions, and separating and collecting by using a stratification phenomenon caused by activity difference of X and Y sperms, the concentration of the R848 in the separation liquid is 0.3 [mu] M to 0.7 [mu] M; according to the specific reproductive physiological characteristics of the yak sperms, the problem that the separation efficiency is low or the sperms are seriously damaged due to inapplicable parameters in the prior art is solved, and the technical scheme special for yak sex control is easy and convenient to operate, low in cost, high in separation efficiency and small in influence on the sperm quality.

A sperm sorting device is disclosed. The sperm sorting device comprises a substrate having a functionalized surface, the functionalized surface comprising a sperm selection agent capable of sorting sperm based on at least one biological or chemical property of the sperm when the sperm is in contact with the functionalized surface.

This makes it possible to select high-quality sperm suitable for intracytoplasmic sperm injection (ICSI) from among the sperm in a sample. To provide a sperm sorting assistance device. [Solution] Calculate at least one indicator related to sperm quality recognized in the video. An index calculation unit and, based on at least one index, during a first time interval in the video The first sperm recognized, and in a second time interval different from the first time interval in the video image An evaluation unit that relatively evaluates the quality of the second sperm recognized by the first time in the video During the interval, information regarding at least one indicator of the first sperm for the first sperm. The relationship between the first sperm and the second sperm, as evaluated by the evaluation unit, is displayed. A sperm sorting assistance device comprising a display control unit that displays an evaluation of relative quality.