The invention discloses a universal animal sperm sorting method which is characterized by comprising the following specific steps: collecting a seminal fluid of an animal to be sorted, mixing the seminal fluid with sperm diluent, putting the mixture into a storage bottle after uniform mixing, and putting the storage bottle into a heat preservation box with the heat preservation temperature of 15-20 DEG C, 2, adding the sorting liquid into the storage bottle, standing for 1 h, then layering, by a water pumping device, pumping out an upper-layer solution containing Y sperms, and the remaining the lower-layer solution containing X sperms and 3, adding an extracting solution into the lower-layer solution, extracting the X sperms from the sorting liquid, and sub-packaging in 100 mL of sub-packaging bottles to obtain a solution containing X sperms. The invention further discloses an X sperm quality control method, a series of operations such as sperm collection, sperm dilution, sperm sorting, sperm extraction and sperm quality control can be effectively completed, and the X sperm quality control method is suitable for livestock sex control in animal husbandry and breeding industry.