26 results about "Sperm sorting" patented technology

To provide a sperm selection assisting device, a projection device, a microscope system, etc. that efficiently assist in selecting good sperm suitable for intracytoplasmic sperm injection from sperm in a specimen. [Solution] A sperm sorting assistance device comprising: an acquisition unit that acquires an observation image of sperm; a reception unit that accepts a sperm evaluation method; an evaluation unit that evaluates the sperm contained in the acquired observation image based on the evaluation method; and a display control unit that controls a projection device provided in the microscope to display, on an observation image presentation unit in the microscope, a composite image in which the evaluation results by the evaluation unit for at least one sperm in the observation image are displayed corresponding to each sperm, and which further displays the evaluation method.

Systems for sorting sperm include a housing and a microfluidic system supported by the housing. The systems also include two or more inlets providing access to the microfluidic system to deliver sperm or semen and fluid to the microfluidic system, as well as an outlet for harvesting sorted sperm. The systems include a flow channel that provides a flow path for sperm from an inlet to an outlet while sperm travels against a fluid flow towards an outlet for harvesting. In the systems, fluid delivered to the microfluidic system via an inlet flows from the inlet towards one or more outlets, and the flow channel extending along the length of the microfluidic system provides a flow path for motile sperm to travel against the fluid flow towards a collection outlet for harvesting. In some embodiments of a system, the microfluidic system also includes a microporous filter arranged in the flow path between the inlet and the collection outlet to cause motile sperm traveling against the fluid flow to pass through the filter to reach the collection outlet for harvesting.

To provide a sperm selection aid that enables the selection of good quality sperm suitable for intracytoplasmic sperm injection (ICSI) from among the sperm in a sample. [Solution] A sperm sorting assistance device comprising: an index calculation unit that calculates at least one index related to the quality of sperm recognized in a video; an evaluation unit that relatively evaluates the quality of first sperm recognized in a first time interval in a video and second sperm recognized in a second time interval different from the first time interval in a video, based on at least one index; and a display control unit that displays information relating to at least one index of the first sperm to the first sperm in the first time interval in a video, and displays the relative quality evaluation of the first sperm and the second sperm evaluated by the evaluation unit.

The invention relates to the technical field of frozen sperms, in particular to a cattle sex control frozen sperm preservation method which comprises the following steps: collecting bull semen, and purifying the bull semen to obtain a sperm suspension so as to determine the qualification of the quality of the semen; performing sperm sorting on the sperm suspension by using a flow cytometry to separate target sperms based on a determination result that the quality of the sperm is qualified; uniformly mixing the target sperms with a refrigerating fluid, standing for a preset period of time, and freezing; obtaining a particle size distribution curve and an ROS concentration curve of the target sperm in the freezing process, and determining the qualification of the temperature drop rate according to the distribution proportion of the particle size distribution curve; adjusting the temperature drop rate according to a comparison result of an ROS fluctuation index of the ROS concentration curve and a preset index on the basis of a judgment result that the temperature drop rate is unqualified; and unfreezing and resuscitating the frozen target sperms to determine the sperm malformation rate so as to adjust the preset activity or the preset index. According to the invention, the quality of frozen sperms is improved.

This makes it possible to select high-quality sperm suitable for intracytoplasmic sperm injection (ICSI) from among the sperm in a sample. To provide a sperm sorting assistance device. [Solution] Calculate at least one indicator related to sperm quality recognized in the video. An index calculation unit and, based on at least one index, during a first time interval in the video The first sperm recognized, and in a second time interval different from the first time interval in the video image An evaluation unit that relatively evaluates the quality of the second sperm recognized by the first time in the video During the interval, information regarding at least one indicator of the first sperm for the first sperm. The relationship between the first sperm and the second sperm, as evaluated by the evaluation unit, is displayed. A sperm sorting assistance device comprising a display control unit that displays an evaluation of relative quality.

An automated sperm sorting device, comprising an original semen tray, a chip tray, a heat insulation block, two horizontal slide rails, two longitudinal slide rails, a connecting rod, a plurality of suction devices, and a controller. The original semen tray comprises a first tray body and a first temperature controller. The first temperature controller is controlled by the controller to provide a first temperature to a first upper surface of the first tray body. The chip tray is provided on one side of the original semen tray, and comprises a second tray body, a sorting set, and a second temperature controller. The sorting set is provided in a chip slot of the second tray body. The second temperature controller is controlled by the controller to provide a second temperature to a second upper surface of the second tray body. The heat insulation block is provided between the first tray body and the second tray body. By means of the two horizontal slide rails and the two longitudinal slide rails, the controller controls the connecting rod so as to move the plurality of suction devices arranged on the connecting rod to different positions.

The invention provides a sika deer sperm sorting solution, a sika deer Y sperm sorting kit based on the sorting solution and application, and relates to the technical field of livestock breeding. The sika deer sperm sorting liquid comprises a sorting basic liquid and a Y sperm sorting functional component, wherein the Y sperm sorting functional component is prepared from rasimod and hyaluronic acid. Rescimod in the sika deer sperm sorting liquid I can be specifically combined with a sika deer X sperm TLR7 / TLR8 membrane receptor to inhibit the movement ability of the sika deer X sperm TLR7 / TLR8 membrane receptor, and a Y sperm is not affected due to lack of the receptor; on the basis, hyaluronic acid in the sorting liquid I and the sorting liquid II forms a physical barrier by increasing the viscosity of the sorting liquid, X sperms with limited movement and Y sperms with insufficient activity are effectively blocked, the Y sperms with high activity cross a barrier layer formed by the sorting liquid I and the sorting liquid II to enter a collecting liquid, then efficient sorting of the functional Y sperms of the sika deer is successfully achieved, and the sorting efficiency of the functional Y sperms of the sika deer is improved. The high-purity and high-activity Y sperms are enriched.

The present invention provides a bovine sex-sorted sperm sorting solution mediated by a small molecule compound and its application. The sperm sorting solution comprises a sperm sorting protection solution and a sex control functional component. The sperm sorting protection solution contains sodium chloride, potassium chloride, calcium chloride dihydrate, sodium dihydrogen phosphate, glucose, sodium pyruvate, sodium bicarbonate, penicillin-streptomycin, and phenol red. The sex control functional component includes creatine and a 24e drug. The concentration of creatine is 450 μM-550 μM, and the concentration of the 24e drug is 0.27 μM-0.33 μM. The 24e drug is a pyrido [3,2-d]pyrimidine-based TLR7&8 dual agonist. The present invention can meet the large demand for sex-sorted semen in production. Moreover, the sorting process is carried out under the suitable conditions for sperm and does not require complex treatment of sperm. It takes less time for sorting and causes less damage to sperm.

This invention discloses a porcine sperm-specific antibody, the coding sequence of which is shown in any one of SEQ ID No: 1-3. This invention prepares a nanobody library by immunizing alpacas with whole porcine sperm. The library can then be screened to identify antibodies that specifically bind to X or Y sperm, thus achieving X and Y sperm sorting. Furthermore, the screened antibodies are nanobodies, only 10% the size of traditional antibodies, with a simpler structure, high stability, strong specificity, and low immunogenicity. This allows for large-scale antibody expression, significantly reducing antibody production costs and facilitating industrial application.

The invention relates to the technical field of sperm sorting, in particular to a goat X and Y sperm sorting solution, a sorting reagent and a sorting method. The X and Y sperm sorting liquid takes water as a solvent, and comprises the following components by concentration: 24-27 g / L of glucose, 15-18 g / L of citric acid, 14-17 g / L of sodium ethylene diamine tetracetate, 55-59 g / L of sodium chloride, 19-22 g / L of sodium bicarbonate, 32-35 g / L of potassium chloride, 5.6-6.0 g / L of Tris, 0.4-0.8 g / L of BSA and 1.0 [mu] mol / L of requinimod R848. The R848 regulates and controls the movement performance of the goat X sperms, so that not only is the molecular effect of the R848 on an interaction network and phosphorylation modification of X sperm metabolites clarified, but also a brand new thought and theoretical basis are provided for precise optimization and safety evaluation of a sperm sorting technology.

The present disclosure provides a sperm sorting assistance device that enables selection of high-quality sperm suitable for intracytoplasmic sperm injection from among sperm in a specimen. The present disclosure provides a sperm sorting assistance device comprising: an index calculation unit that calculates at least one index relating to the quality of sperm recognized in a moving image; an evaluation unit that, on the basis of the at least one index, relatively evaluates the quality of first sperm recognized during a first time interval in the moving image and second sperm recognized during a second time interval different from the first time interval in the moving image; and a display control unit that, in the first time interval in the moving image, displays information relating to the at least one index of the first sperm in association with the first sperm, and displays an evaluation of the relative quality of the first sperm and the second sperm evaluated by the evaluation unit.

This invention relates to microfluidic devices, and more particularly to a sperm sorting device and a sperm guiding device. A sperm sorting device is provided, comprising: an inlet chamber, an outlet chamber located radially inside the inlet chamber, and a channel connecting the inlet and outlet chambers. The channel includes blind branches with a radius of curvature between 50 μm and 75 μm. A sperm guiding device is also provided, comprising an inlet opening and an outlet opening, and blind branches with a radius of curvature between 50 μm and 75 μm. The technical advantage of this invention is the reduction of sperm backflow.

[Problem] To improve a sperm yield. [Solution] A sperm sorting device 1 comprises: a bottom wall 11; a side wall 12; and an internal space 13 defined by the bottom wall 11 and the side wall 12. The internal space 13 has: an input part 31; a collection part 32; and a communication part 33 for connecting the input part 31 and the recovery part 32. The communication part 33 includes: a bottom surface 43; and an inner wall 44 extending upward from the bottom surface 43 and across the input part 31 and the recovery part 32. Motile sperm 200 introduced into the input part 31 move to the collection part 32 along the bottom surface 43 of the communication part 33 and a surface of the inner wall 44. Even motile sperm 200 with a low acceleration against gravity can move from the input part 31 to the collection part 32 through the communication part 33.

A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

The present invention discloses a sperm sorting fluid based on sperm taxis and a preparation method thereof. The sperm sorting fluid based on sperm taxis comprises the following components: Liquid A: selected from a sperm culture fluid, sperm capacitation fluid or human fallopian tube fluid; Component B: selected from a sodium alginate, hydroxyethyl cellulose, hyaluronic acid, dextran or polyethylene glycol, with a final concentration of 5-25 mg / mL; Component C: selected from a sodium bicarbonate, potassium bicarbonate or basic amino acid, with a final concentration of 2.5-12.5 mg / mL. The components work together to simulate the physicochemical properties of cervical mucus in vitro, simulate the sperm selection microenvironment in vitro, sort active sperm based on sperm taxis, effectively improve the active sperm rate and the normal sperm morphology rate, and reduce sperm DFI.

The present invention relates to the technical field of sperm sorting, and provides a sperm processing chip and a use method therefor. The sperm processing chip comprises: a second tank arranged in a first tank, wherein a sample adding pool for placement of a sperm sample to be tested is formed between the first tank and the second tank, an outer wall surface of the second tank faces the sample adding pool, the top of the outer wall surface is constructed as a slope, one end of the slope points to the sample adding pool and the other end is connected to the interior of the second tank, the slope is provided with a microchannel extending in an inclined direction of the slope, an inner wall surface of the second tank forms a collection pool for collecting sperm that flows from the sample adding pool to the microchannel of the slope and falls into the collection pool, and the tank wall height of the first tank is higher than the tank wall height of the second tank. The sperm processing chip can achieve active sorting without the use of chemicals, and sperm screening under the action of chemicals, can effectively maintain sperm vitality, and has the advantages of high versatility, high recovery rate, high sorting accuracy, high motility rate, and ease of operation.

A sperm sorting device is suitable for sorting a plurality of motile sperm from a semen sample, and includes a base and a first sorter positioned on the base. The base includes a receiving space defined by a bottom wall and a surrounding wall. The first sorter includes a first kit and a first permeable membrane. The first kit has a first sleeve for being placed into the receiving space. The first sleeve has a first inner sleeve surface and a first outer sleeve surface. The first permeable membrane is fixed to the first sleeve. The first permeable membrane and the inner wall surface of the bottom wall and the surrounding wall define a first sample receiving chamber for receiving the semen sample. In this way, the motile sperm can naturally swim upward and pass through the first permeable membrane, and the quality of sperm sorting can be improved.

The application relates to a sperm sorting device, which comprises a screening unit, a guiding unit and a storage unit connected in sequence from bottom to top, the screening unit is internally provided with microchannels and an annular channel, the microchannels are distributed radially with the axis of the screening unit as the center, the ends of the microchannels close to the axis of the screening unit meet each other and form a meeting area, the ends of the microchannels away from the axis of the screening unit are communicated with the annular channel, the screening unit is provided with an injection port communicated with the annular channel, the guiding unit is provided with a guiding cavity, and the storage unit is provided with a storage cavity. Sperms enter from the injection port, high-activity sperms in linear motion can quickly pass through the microchannels to enter the meeting area, preliminary sorting is realized, the sperms entering the meeting area move into the storage cavity in the upper direction under the guiding action of the guiding cavity, high-activity sperms can be further screened out, and finally the sperms screened out are stored in the storage cavity. Strongly active and high-quality sperms can be quickly and accurately screened out, and the working efficiency is improved.

This invention relates to the field of sperm sorting technology, specifically to a sperm processing chip and its usage method. The sperm processing chip includes: a second tank disposed within a first tank, forming a sample loading chamber between the first and second tanks for placing sperm samples to be tested; the outer wall of the second tank faces the sample loading chamber, and the top of the outer wall is sloped, with one end of the slope pointing towards the sample loading chamber and the other end connected to the interior of the second tank; microchannels extending along the slope's inclination direction are formed on the slope; and a collection chamber is formed on the inner wall of the second tank for collecting sperm that swim from the sample loading chamber to the slope microchannels and fall into the collection chamber; the height of the first tank wall is higher than that of the second tank wall. This sperm processing chip can achieve active sorting without chemical drugs and sperm screening under drug action, and can effectively maintain sperm activity. It has the advantages of high versatility, high recovery rate, high sorting accuracy, high motility, and ease of operation.

The invention discloses a diluent for sorting pig Y sperms as well as a preparation method and application of the diluent. The diluent is prepared from the following components: 40 g / L of glucose, 0.5 g / L of sodium citrate, 0.1 g / L of creatine monohydrate, 0.75 g / L of potassium chloride, 0.0015 g / L of rotenone, 2.38 g / L of HEPES, 0.2 g / L of magnesium chloride and 1.0 g / L of bovine serum albumin. The diluent can amplify the difference of linear movement speed between X and Y sperms of pigs by regulating ATP supply, and can separate the Y sperms with higher movement speed in combination with an upstream sorting method, and the separation efficiency reaches 70-80%. The diluent disclosed by the invention has the characteristics of simplicity, rapidness, high efficiency and low cost in Y sperm separation, is almost harmless to sperms, and is suitable for being applied to boar production.

The invention relates to a yak X / Y sperm separation and enrichment method based on pH value regulation and a kit thereof, and belongs to the technical field of animal breeding and sex control. The method comprises the following steps: using an Optidyl diluent as a sperm sorting protection solution, adjusting the pH value of the Optidyl diluent through NaOH or HCl, enriching X sperms in a pH range of 6.2-6.4, and enriching Y sperms in a pH range of 6.8-7.0; the method comprises the following specific steps: diluting yak sperm, centrifugally desizing, re-suspending by using a sorting solution with corresponding pH, standing and incubating for 45-90 minutes at 35-40 DEG C and 45 DEG C, realizing natural layering according to the motility difference of X and Y sperms under different pH conditions, respectively collecting upper or lower sperms, and washing and re-suspending to obtain a sex-controllable sperm sample; the method is easy and convenient to operate, low in cost, small in sperm damage, complete in sperm structure and stable in function after separation, suitable for breeding technologies such as artificial insemination and in-vitro fertilization, capable of meeting the requirement for sexual control sperm in yak production and beneficial for optimizing the population structure and improving the breeding efficiency.

The invention provides a sheep X and Y sperm separating medium and a sorting method, and belongs to the technical field of biology. The separation liquid comprises a separation liquid, a washing liquid and requinimod R848. Wherein the washing liquid contains a protective agent, namely oroxin B, for preventing the goat sperms from being damaged by R848, so that the movement performance of the separated goat sperms can be improved, and the movement performance such as the sperm motility, the linear rate, the path rate and the linearity of the goat can be remarkably improved. In addition, the conditions of an X and Y sperm sorting system are optimized, and the X and Y sperms of the goat can be efficiently separated. The purity of X sperms sorted by the method can reach 88.1%, the proportion of female embryos after in-vitro fertilization reaches 80%, the purity of Y sperms can reach 85.7%, and the proportion of male embryos after in-vitro fertilization reaches 84%.

The invention relates to the technical field of animal breeding, and discloses a yak X / Y sperm separation and enrichment method based on a TLR7 / 8 agonist R848 and a kit. The method comprises the following steps: treating the yak sperm by using a sperm sorting solution containing a TLR7 / 8 dual agonist R848, incubating under specific conditions, and separating and collecting by using a stratification phenomenon caused by activity difference of X and Y sperms, the concentration of the R848 in the separation liquid is 0.3 [mu] M to 0.7 [mu] M; according to the specific reproductive physiological characteristics of the yak sperms, the problem that the separation efficiency is low or the sperms are seriously damaged due to inapplicable parameters in the prior art is solved, and the technical scheme special for yak sex control is easy and convenient to operate, low in cost, high in separation efficiency and small in influence on the sperm quality.

A sperm sorting device is disclosed. The sperm sorting device comprises a substrate having a functionalized surface, the functionalized surface comprising a sperm selection agent capable of sorting sperm based on at least one biological or chemical property of the sperm when the sperm is in contact with the functionalized surface.

This makes it possible to select high-quality sperm suitable for intracytoplasmic sperm injection (ICSI) from among the sperm in a sample. To provide a sperm sorting assistance device. [Solution] Calculate at least one indicator related to sperm quality recognized in the video. An index calculation unit and, based on at least one index, during a first time interval in the video The first sperm recognized, and in a second time interval different from the first time interval in the video image An evaluation unit that relatively evaluates the quality of the second sperm recognized by the first time in the video During the interval, information regarding at least one indicator of the first sperm for the first sperm. The relationship between the first sperm and the second sperm, as evaluated by the evaluation unit, is displayed. A sperm sorting assistance device comprising a display control unit that displays an evaluation of relative quality.

A sperm sorting device and a sperm sorting method are provided. The sperm sorting device comprises a collection tank, a waste liquid tank, a sorting flow channel, and a material layer. The waste liquid tank is located on a side of the collection tank. The sorting flow channel extends between the collection tank and the waste liquid tank. The sorting flow channel is in communication with the collection tank and the waste liquid tank. The sorting flow channel comprises a lower surface, an upper surface, and a sidewall. The sidewall is located between the lower surface and the upper surface. The sidewall is connected to the lower surface and the upper surface. The material layer is located on the lower surface and the upper surface of the sorting flow channel. The material layer has a negative potential or a positive potential. The range of the negative potential is -1 millivolt to -30 millivolts. The range of the positive potential is 1 millivolt to 30 millivolts. The described sperm sorting device can effectively separate X sperm from Y sperm, and the separated X sperm and Y sperm can have relatively high activity.