2979 results about "Liposome" patented technology

To provide a technique for enhancing the dermal delivery of a composition and, furthermore, enhancing the delivery of the composition to macrophage-surrounding areas.SOLUTION: The present invention provides a liposome with a particle diameter of 300 nm or less, comprising (A) polyglycerin fatty acid ester, (B) phospholipid, and (C) sterol. In a preferred embodiment, the liposome further comprises (D) an amphiphilic molecule having a disaccharide-to-trisaccharide hydrophilic group and an alkyl-chain hydrophobic group within its molecular structure.SELECTED DRAWING: None

The invention relates to a mild exfoliating whitening and repairing body care composition as well as a preparation method and application thereof. The mild exfoliating whitening and repairing body care composition is prepared from the following components in parts by weight: 5 to 10 parts of lipidosome, 0.01 to 1 part of papain and 0.005 to 0.5 part of bromelain, wherein the liposome comprises a shell layer and a core layer, the shell layer comprises lecithin and phytosterol, and the core layer comprises a golden camellia extract and hydrolyzed royal jelly protein; the liposome is prepared from the following raw materials in parts by weight: 2 to 15 parts of lecithin, 0.1 to 5 parts of phytosterol, 0.1 to 3 parts of golden camellia extract and 0.01 to 1 part of hydrolyzed royal jelly protein. The composition can realize multiple effects of brightening skin color, removing waste cutin and strengthening skin, and is safe, mild and non-irritant.

The invention relates to the technical field of immunology, and discloses a QS-21 composite adjuvant as well as a preparation method and application thereof, and the QS-21 composite adjuvant comprises the following components in parts by weight: 0.1-0.5 part of QS-21; 4 to 6 parts of liposome; 0.05 to 0.1 part of CpG oligonucleotide; 0.1 to 0.3 part of a freeze-drying protective agent; 0.2 to 0.5 part of a stabilizing agent; 3 to 5 parts of propylene glycol; 1-2 parts of ethanol; 5-15 parts of an organic solvent chloroform; the liposome is prepared from the following components in parts by weight: 3 to 4 parts of phospholipid distearate; and 1-2 parts of amino lipid. By compounding the liposome carrier and the QS-21, adding the CpG oligonucleotide and optimizing the freeze-drying protective agent, the loading efficiency of the immunologic adjuvant, the immune effect stability and the restorability after freeze-drying are improved, and the durability and efficacy of immune response are enhanced.

The invention provides temperature and pH responsive hydrogel entrapped with chlorogenic acid lipidosome as well as a preparation method and application of the temperature and pH responsive hydrogel, and relates to the technical field of biology. The temperature and pH responsive hydrogel entrapping chlorogenic acid lipidosome contains chlorogenic acid lipidosome, phenylboronic acid grafted modified gelatin and dopamine grafted oxidized hyaluronic acid, the concentration of the phenylboronic acid grafted modified gelatin is 3-10 wt%, the concentration of the dopamine grafted oxidized hyaluronic acid is 3-10 wt%, and the concentration of chlorogenic acid is 1-20 [mu] M. The temperature and pH responsive hydrogel can effectively promote wound healing independently or in combination with blue light.

The invention discloses a recombinant herpes zoster vaccine as well as a preparation method and application thereof, and particularly relates to the technical field of biological medicines. The recombinant herpes zoster vaccine comprises a recombinant herpes zoster vaccine fusion protein purified product HN101 and a liposome mixture HA201, and the recombinant herpes zoster fusion protein gE-gD is constructed by recombining reconstructed gE protein and reconstructed gD protein. The gE-gD recombinant herpes zoster fusion protein is obtained by reconstructing gE protein of varicella-herpes zoster virus and gD protein of HSV through a gene recombination technology and then recombining the gE protein and the gD protein. After the gE-gD recombinant herpes zoster fusion protein is combined with a liposome mixture HA201, stronger immune response can be induced, the antibody titer and cellular immunity are improved, the vaccine effect is enhanced, and meanwhile, the potential infection risk is avoided.

The invention discloses multifunctional hydrogel and application thereof, and belongs to the technical field of biological medicine. According to the multifunctional hydrogel, ketal mercaptan with two hydrazide ends and oxidized dextran are cross-linked to form a cross-linked network containing dynamic covalent bonds. The preparation method comprises the following steps: dissolving ketal mercaptan with two hydrazide ends in deionized water to obtain a ketal mercaptan solution, dissolving oxidized dextran in deionized water to obtain an oxidized dextran solution, mixing the ketal mercaptan solution with the oxidized dextran solution, and standing to obtain the multifunctional hydrogel. The multifunctional hydrogel has pH / ROS dual responsiveness, can respond to inflammation microenvironment and low pH of infectious wounds, remove active oxygen, release therapeutic drugs, regulate macrophage polarization and relieve bacterial infection, thereby playing a key role in inflammation, proliferation and remodeling stages of wound healing to accelerate wound healing. The curcumin-encapsulated liposome is added into the multifunctional hydrogel, so that the antibacterial effect can be further improved.

The invention provides a preparation method of lipidosome containing hair-growing active ingredients, and belongs to the technical field of hair-growing products. According to the invention, active substances are utilized to regulate hormone level, improve scalp blood circulation, stimulate anti-hair loss factors and promote hair growth in multiple directions, and lipidosome formed by lecithin and oil-soluble active ingredients as raw materials is utilized to integrally entrap the active substances, so that the anti-hair loss effect is improved. The defect of weak transdermal ability caused by large water solubility difference among the active substances is overcome, the stability and bioavailability of the active substances can be improved, and the hair growth and development effects of the prepared lipidosome containing the hair growth active components are remarkably improved.

The invention discloses a ginsenoside liposome. The ginsenoside liposome comprises ginsenoside, phospholipid and a surfactant, and the ginsenoside comprises protopanoxadiol or / and protopanaxatriol. The invention also discloses a preparation method of the ginsenoside liposome and application of the ginsenoside liposome in preparation of medicines, cosmetics or skin care products. According to the invention, the final desugared product of ginsenoside with the effect of treating skin toxicity reaction is prepared into the liposome, so that the membrane permeation and absorption capabilities of ginsenoside are greatly improved, the skin toxicity caused by tumor treatment can be quickly and effectively relieved, and the drug effect can be played for a long time; the invention provides a new method for treating skin toxic reaction caused by tumor treatment, and makes up for the requirements which cannot be met by the existing treatment means.

The invention provides a preparation method of mitochondria-targeted antioxidant hybrid vesicles. The preparation method comprises the following steps: S1, preparing and separating adipose-derived stem cell nano-vesicles; s2, preparing a liposome loaded with dihydromyricetin and distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide by an ethanol injection method; and S3, preparing the hybrid nano vesicles loaded with the dihydromyricetin and the distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000-triphenylphosphine targeting peptide. The invention also provides the hybrid vesicles prepared by the method and application of the hybrid vesicles in preparation of refractory diabetes wound treatment drugs. The lipidosome and the adipose-derived stem cell nano-vesicles are hybridized to prepare the hybridized vesicles capable of targeting mitochondria and resisting oxidation, the hybridized vesicles are applied to wound treatment for the first time, the problem of oxidative stress of diabetic wounds is solved, wound healing is accelerated, and a new strategy is provided for optimizing mitochondrial function defects and oxidative stress of the diabetic wounds.

The invention relates to the technical field of cosmetics, in particular to an acne-removing composition, lipidosome and a preparation method and application thereof. The acne-removing composition disclosed by the invention is prepared from the following components in parts by weight: 2 to 8 parts of nicotinamide, 1 to 5 parts of cortex magnoliae officinalis extract, 0.15 to 2.01 parts of glycyrrhizic acid, 0.1 to 2 parts of totarol and 0.01 to 0.15 part of hyssopus officinalis extract. The nano-carrier is adopted to wrap and deliver the acne removal composition, so that the solubility of the functional components is increased, the bioavailability of the functional components is improved, meanwhile, the irritation of the functional components is also improved, multi-target co-delivery and multi-mechanism acne removal are realized, and the acne removal composition has a remarkable acne removal effect.

The invention relates to the technical field of pharmaceutical chemicals, and discloses sequential drug release liposome hydrogel and a preparation method and application thereof, and the preparation method of the liposome hydrogel comprises the following steps: step 1, preparation of drug-loaded positive charge liposome; step 2, preparing phenylboronic acid modified polysaccharide; and step 3, carrying out vortex mixing on the drug-loaded positive charge liposome solution, the phenylboronic acid modified polysaccharide hydrogel precursor powder and the spermidine solution to obtain the liposome hydrogel. According to the sequential drug release liposome hydrogel as well as the preparation method and the application thereof, the liposome hydrogel is prepared by taking high-molecular hyaluronic acid and phenylboronic acid as main raw materials, and the liposome hydrogel has microenvironment responsiveness and time-space characteristics of drug release, and can be used for treating osteoarthritis in an environment with active oxygen increase, so that the drug release effect is good, and the drug release effect is good. The functions of early-stage anti-inflammation and continuous cartilage regeneration are achieved by sequentially releasing double drugs, synovitis can be effectively relieved, cartilage regeneration can be promoted, and the compound can be used for preparing drugs for treating osteoarthritis.

The present invention relates to sonosensitive liposomes and a method for preparing the same. The sonosensitive liposomes according to the present invention improve the delivery efficiency of encapsulated drugs, and are expected to exhibit various functions in the fields of cancer treatment and drug delivery carriers by improving drug delivery effects through targeted cancer cells when used in combination with ultrasonic treatment.

The invention discloses a liver-targeting drug liposome and application thereof, and relates to the field of drug delivery, cholate and mannose are used for respectively modifying phospholipid molecules DSPE-PEG2000, two raw materials DSPE-PEG2000-CA and DSPE-PEG2000-MAN composed of the liposome are synthesized, and then the liposome is prepared into the liver-targeting drug liposome. The liposome with liver targeting property is obtained from the liposome, cholesterol, DSPC, DC-cholesterol and a water-soluble therapeutic drug according to a certain proportion, the liposome has relatively high cell safety and liver targeting property, and the therapeutic effect of the corresponding drug can be remarkably improved after the drug is embedded.

The invention discloses a high-pathogenicity porcine reproductive and respiratory syndrome virus mRNA vaccine composition and application thereof. The vaccine composition is selected from any one of the following combinations: (1) an mRNA vaccine composition consisting of a PRRSV GP3 mRNA (messenger ribonucleic acid), a PRRSV GP4 mRNA (messenger ribonucleic acid) and a PRRSV GP5 mRNA (messenger ribonucleic acid); or (2) an mRNA vaccine composition consisting of a PRRSV GP5 mRNA, a PRRSV membrane protein (M) mRNA and a PRRSV nucleocapsid protein (N) mRNA. According to the invention, a high-pathogenicity PRRSV JX-A1 strain is used as a template, and mRNAs capable of synthesizing a plurality of fusion proteins GP345, GP5M and GP5MN and single structural proteins GP3, GP4, GP5, M and N are respectively constructed. After liposome encapsulation, mice are inoculated for immunological evaluation, and results show that humoral immunity and cellular immunity levels of a single mRNA-LNPs mixed group are higher than those of recombinant mRNA-LNPs, and GP5 + M + N and GP3 + 4 + 5 have relatively best effects. The invention provides a new technical means for preventing PRRSV (Porcine Reproductive and Respiratory Syndrome Virus).

The invention discloses a GPC3 derived antigen peptide vaccine, an mRNA vaccine, a saRNA vaccine and a preparation method and application thereof. The amino acid sequence of the antigen peptide vaccine is one or any combination of more of amino acid sequences shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6. According to the invention, an efficient pGPC3 model antigen peptide is screened on the basis of GPC protein, and pGPC3 mRNA and pGPC3 saRNA are designed according to a pGPC3 sequence example. The pGPC3saRNA (at) Lipi vaccine is prepared through a liposome technology, and due to the fact that limiting conditions such as nonapeptide and high-affinity binding with H-2I type molecules (H-2Kb) are adopted during pGPC3 polypeptide antigen screening, APC cells are facilitated to present antigens through an MHCI restrictive pathway, CD8 + T cells are activated, and GPC3 specific CTL is triggered to efficiently kill liver cancer cells. Finally, the pGPC3saRNA (at) Lipi vaccine can completely inhibit the growth of the liver cancer, the inhibition rate reaches 99.8%, and the liver cancer of 4 / 8 mice completely fades down. In addition, the method can be used for vaccine adjuvant and carrier research on a liver cancer subcutaneous transplantation tumor model.

The invention provides a composition for promoting hair regeneration after chemotherapy as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: modifying the surface of a gold nanocage with polydopamine, loading the modified gold nanocage on an exosome, complexing magnesium ions and zinc ions to prepare a modified exosome, preparing a nano drug-loaded liposome from curcumin, resveratrol and epigallocatechin gallate, mixing the nano drug-loaded liposome with the modified exosome to prepare a multi-layer liposome, and preparing the multi-layer liposome from the nano drug-loaded liposome. And mixing with a penetration enhancer, and adding into a temperature-sensitive hydrogel system to prepare the composition for promoting hair regeneration after chemotherapy. The composition for promoting hair regeneration after chemotherapy has a good transdermal absorption promoting effect, can slowly release drugs, promotes hair follicles to be converted from a resting stage to a growing stage under the synergistic effect of the drugs, regulates and controls multiple pathways (such as anti-apoptosis, angiogenesis promotion and anti-inflammation) at the same time, is matched with a complex pathological mechanism of hair regeneration after chemotherapy, and can promote hair regeneration after chemotherapy. Wide application prospects are realized.

The invention provides a preparation method and application of a high-efficiency encapsulated pH-responsive drug-loaded liposome. According to the method, two-phase fluid of a medicine aqueous solution and a lipid component organic solution in a micro-channel is accurately controlled, and the efficiently encapsulated liposome is prepared from bottom to top by utilizing a micro-mixing technology. Compared with a conventional passive drug loading method which is relatively low in encapsulation efficiency in preparation of hydrophilic drug nano-liposomes, the method provided by the invention can improve the encapsulation efficiency to be close to 95% under optimized prescription and process conditions. According to the invention, by adjusting the proportion of each component in the prescription and the preparation process, the release behavior of liposome drugs such as adriamycin and the like can be adjusted, and the drug release shows pH responsiveness. Through efficient encapsulation of adriamycin and the like and pH-responsive release of the liposome dosage form, the effects of efficacy enhancement and toxicity reduction are achieved in the anti-tumor treatment aspect.

The invention discloses a preparation method of an anti-aging cosmetic containing exosome, and relates to the technical field of cosmetic preparation, the cosmetic is prepared from the following components in percentage by mass: 1.01%-5.05% of exosome wrapped by liposome, 0.5%-3% of an antioxidant, 1%-3% of a centella asiatica extract, 1%-3% of a rhodiola rosea extract, 10%-15% of a humectant and the balance of a basic matrix; wherein the exosome wrapped by the liposome is a mixture formed by mixing an exosome secreted by adipose-derived mesenchymal stem cells and an exosome secreted by plant cells according to a mass ratio of 1.5: 1.2; the plant is ginseng. According to the skin care product, the antioxidant, the centella asiatica extract and the exosome act together to improve the DPPH clearance rate of the skin care product, the skin brightness is improved, the roughness is reduced, and the excellent anti-aging performance is achieved. The lipid exosome-antioxidant-centella asiatica ternary system disclosed by the invention has a multi-target synergistic effect.

The invention provides a liposome preparation and a preparation method thereof, the liposome preparation comprises an ion pairing agent, phospholipid or a derivative thereof, sterol, a lipid molecule derivative and a drug compound, the ion pairing agent comprises at least one of a compound (I) and a compound (II), and the compound (II) comprises at least one of a compound (III) and a compound (III). Structures in the compound (I) and the compound (II) contain a plurality of functional groups (such as amide, carbonyl, thioether and the like), so that abundant binding sites are provided for drug molecules, and the compound (I) and the compound (II) can interact with different types of drug compounds (such as fat-soluble and water-soluble drugs); the liposome preparation is allowed to be combined with various drug molecules through hydrogen bonds, hydrophobic interaction or electrostatic interaction, and the liposome preparation adapts to physicochemical properties of different drugs. The compound (I) and the compound (II) further comprise different hydrophobic groups (such as alkyl chains) and hydrophilic groups (such as sulfonic acid, carboxylic acid and the like), so that the liposome preparation has the amphiphilic characteristic, not only can encapsulate hydrophobic drugs, but also can encapsulate hydrophilic drugs, and the variety and range of encapsulated drugs are improved.

The invention discloses a drug-loaded liposome nano-particle, a preparation method thereof and application of the drug-loaded liposome nano-particle in treatment of brain glioma, and belongs to the technical field of biological nano-medicines. The invention provides a novel copper phthalocyanine derivative and catalase co-loaded lipidosome nanoparticle and application of the novel copper phthalocyanine derivative and catalase co-loaded lipidosome nanoparticle to treatment of brain glioma on the cellular level. The nano-particles are obtained by wrapping copper phthalocyanine derivatives and catalase with lipidosome prepared by a microfluidic method. The co-drug-loaded liposome can catalyze excessive H2O2 in brain glioma to generate oxygen, and O2 is continuously supplied to photodynamic therapy (PDT) to generate active oxygen; meanwhile, Cu < 2 + > can deplete glutathione and improve the oxidative stress level in the glioma, the reduction product Cu < + > can convert H2O2 into hydroxyl radicals with higher toxicity through a Fenton-like reaction, oxidative stress injury is doubly amplified, cascade cooperation of PDT and chemical dynamic therapy (CDT) is achieved, and the treatment effect on the glioma is remarkably enhanced.

The invention relates to a STING agonist prodrug compound, a liposome preparation and a preparation method thereof. Specifically, the invention relates to an STING agonist prodrug compound shown in a general formula (I) or a pharmaceutically acceptable salt thereof, a liposome preparation thereof and application of the STING agonist prodrug compound or the pharmaceutically acceptable salt and the liposome preparation in preparation of drugs for treating STING-related diseases. The prodrug compound disclosed by the invention can be rapidly hydrolyzed under the induction of esterase to release an active ingredient MSA-2, and has a good STING protein excitation function. # imgabs0 #

The invention provides a nanoparticle compound for inhibiting bone and soft tissue infection as well as a preparation method and application thereof, and belongs to the technical field of medical biological materials. The nanoparticle compound is a gentiopicroside, sericin, hyaluronic acid and endolysin liposome nanoparticle, and a gentiopicroside, sericin, hyaluronic acid and endolysin co-delivery nano drug delivery system targeting the S100A4 site is designed based on the target S100A4 closely related to bone infection through Ai molecular docking and nano drug delivery technologies. The nanoparticle compound provided by the invention has better stability and bioavailability, and can be used for improving and relieving inflammatory response and increasing wound healing effect.

The invention belongs to the technical field of genetic engineering and biological medicine, and particularly discloses a cascade response type nano-particle with a microenvironment remodeling function and a preparation method and application of the cascade response type nano-particle. The nanoparticle provided by the invention adopts a bionic hybrid membrane engineering strategy: an inner core is formed by loading an exosome inhibitor on a nano material, and the surface is covalently coupled with M2 type macrophage specific targeting peptide; the outer layer coats a macrophage membrane and active oxygen response type liposome composite membrane layer through a co-extrusion technology, and is loaded with a TLR agonist. Animal experiments show that after being injected through caudal vein, the nano-particles are enriched at a tumor site in a targeted manner through homing receptors such as membrane surface integrin alpha4beta1 and the like, and outer membrane cracking is triggered in a high-ROS tumor microenvironment, so that space-time controlled release of a TLR agonist and targeted phagocytosis of an exosome inhibitor by macrophages guided by a targeted peptide are realized. The anti-tumor synergistic effect is achieved through multiple regulation and control mechanisms, and an effective treatment strategy is provided for tumor immunotherapy.

The invention relates to the field of nano-drug delivery preparations, in particular to a traditional Chinese medicine transdermal drug delivery preparation based on a nano-coating technology and a preparation method thereof, the traditional Chinese medicine transdermal drug delivery preparation comprises a liposome-metal organic framework composite carrier, and the composite carrier comprises traditional Chinese medicine active ingredients; a meridian point targeted transdermal enhancer; an iontophoresis device for dynamically sensing skin impedance; the invention relates to an intelligent administration time sequence control system based on a traditional Chinese medicine midnight-noon ebb-flow theory. The liposome-metal organic framework composite carrier is adopted, the similar structure of liposome and skin and the high specific surface area of MOF are fully utilized, efficient encapsulation and transdermal absorption of active ingredients of traditional Chinese medicine are achieved, and compared with a traditional transdermal patch, the drug transdermal absorption rate is increased by 3-5 times.

The invention relates to the technical field of biological materials, in particular to a hydrogel scaffold material for spinal cord injury repair as well as a preparation method and application of the hydrogel scaffold material. The hydrogel scaffold material AP39 (AP39-coated LIP) for spinal cord injury repair is prepared by combining the liposome-coated AP39 (AP39-coated LIP) serving as a drug with a GelMA hydrogel scaffold, and a scaffold is provided for injured spinal cords, so that axon regeneration of injured parts is guided, mitochondria of the injured parts is targeted to release H2S, neuronal injury is relieved, and the spinal cord injury repair effect is improved. Therefore, nerve regeneration and functional recovery after spinal cord injury are promoted.

The invention relates to the technical field of cosmetics, and discloses an anti-inflammatory soothing cosmetic composition which comprises the following components in percentage by weight: 0.5%-4% of a barrier repairing component, 0.5%-3% of an immunoregulation component, 0.3%-2.5% of a nerve soothing component, 0.3%-3% of an anti-inflammatory plant compound, 1%-10% of a moisturizing soothing enhancer and the balance of auxiliary materials. According to the components, through a five-dimensional synergistic mechanism of barrier repair, immune tolerance, nerve desensitization, anti-inflammatory itching relieving, moisturizing and stability maintaining, anti-inflammatory relieving and barrier repair are remarkably enhanced; ceramide, rare ginsenoside, phytosterol ester and free fatty acid are introduced to synergistically construct a bionic liposome which is closer to the composition of human cuticle lipid, so that the transdermal absorption and stability are enhanced, and the repair efficiency is remarkably improved; the dipotassium glycyrrhizinate, the tamarix chinensis flower extract, the purslane extract, the dinoflaxanthine and the baicalin cooperate to quickly resist inflammation and relieve itching and strengthen barrier repair, so that the composition is suitable for highly sensitive skin.

Provided herein are systems for treating a subject with a pulmonary infection, for example, a nontuberculous mycobacterial pulmonary infection, a Burkholderia pulmonary infection, a pulmonary infection associated with bronchiectasis, or a Pseudomonas pulmonary infection. The system includes a pharmaceutical formulation comprising a liposomal aminoglycoside dispersion, and the lipid component of the liposomes consist essentially of electrically neutral lipids. The system also includes a nebulizer which generates an aerosol of the pharmaceutical formulation at a rate greater than about 0.53 gram per minute. The aerosol is delivered to the subject via inhalation for the treatment of the pulmonary infection.

The invention discloses a cationic super-deformation liposome capable of realizing gene transdermal transfection as well as preparation and application of the cationic super-deformation liposome, and belongs to the field of gene transdermal delivery. Materials such as low-molecular-weight polyethyleneimine, lipidosome and Tween 80 are adopted, and the components are safe and simple; polyethyleneimine is used as a conjugation carrier of the gene to improve the binding capacity of the gene, liposome is used as a delivery carrier to improve the skin delivery capacity of the gene, and Tween 80 is used for improving the stability and transdermal transfection capacity of the carrier again, so that non-invasive targeted transdermal delivery of the gene is realized; in vitro stability is high, gene degradation can be effectively inhibited, and meanwhile in vitro cell experiments, high transfection efficiency and low toxicity are achieved; the cationic super-deformed liposome can successfully realize high-efficiency transdermal penetration of the gene and effectively promote transfection expression of the gene in the skin at the same time, a new idea is provided for gene delivery, and the advantage of treating skin diseases through non-invasive delivery of the gene is expected to be fully exerted.

The invention discloses a novel PDRN, a supramolecular liposome containing the PDRN as well as a preparation method and application of the supramolecular liposome. The preparation method of the novel PDRN comprises the following steps: (1) mixing a tissue raw material and PBS, crushing, carrying out enzymolysis, adding SDS, a Tween20 solution, polyethylene glycol octylphenol ether, a TE buffer solution and NaCl, stirring, centrifuging, mixing a supernatant with absolute ethyl alcohol, and standing to obtain a DNA crude extract; (2) carrying out breaking shearing treatment by using an ultrasonic breaking instrument; and (3) carrying out enzymolysis treatment by using restriction endonuclease and deoxyribonuclease, and the like. The PDRN supramolecular liposome is obtained by wrapping the PDRN with fatty acid extracted from marine microalgae. The fish testis are used as the raw material source, the selection range of the raw materials is widened, the preparation cost is reduced, and the organic reagent used in the preparation method is ethyl alcohol which is easy to remove and harmless.