12 results about "Etioplasts" patented technology

The invention discloses a method for converting a carbon source into ethylene glycol. The method comprises the following steps: providing a plastid, wherein the plastid comprises gene sequences of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5 and SEQ ID NO: 6; implanting the plastid into blue-green bacteria by means of electroporation treatment to obtain modified blue-green bacteria; and providing the carbon source to the modified cyanobacteria, so that the modified cyanobacteria converts the carbon source into ethylene glycol, thereby producing chemicals with high economic value while treating the carbon-containing waste gas.

The invention provides methods of using chloroplast-selectable markers to detect nuclear genome editing. The methods involve selecting a genetic modification that has occurred in a nuclear genome of a photosynthetic cell; transforming a photosynthetic cell with a ribonucleoprotein complex that effects a genetic modification in the genome in the nucleus of a photosynthetic cell; transforming the photosynthetic cell with a construct that effects an insertion of a selectable marker into the plastome of a plastid of the cell; selecting for photosynthetic cells having the selectable marker inserted into the plastome; and thereby selecting for the genetic modification to the genome in the nucleus of the photosynthetic cell.

Compositions and methods for introducing targeted mutations into the plastid genome are provided.

The invention provides methods of using chloroplast-selectable markers to detect nuclear genome editing. The methods involve selecting a genetic modification that has occurred in a nuclear genome of a photosynthetic cell; transforming a photosynthetic cell with a ribonucleoprotein complex that effects a genetic modification in the genome in the nucleus of a photosynthetic cell; transforming the photosynthetic cell with a construct that effects an insertion of a selectable marker into the plastome of a plastid of the cell; selecting for photosynthetic cells having the selectable marker inserted into the plastome; and thereby selecting for the genetic modification to the genome in the nucleus of the photosynthetic cell.

The invention relates to liver-targeted lycopene plastid pellets and application thereof, the problems that lycopene is poor in water solubility, easy to oxidize and degrade and the like can be solved through the nanoscale structure of plastid membranes wrapping lycopene of the plastid pellets, and the liver-targeted lycopene plastid pellets serve as a natural delivery system. The plastid pellets can improve the water solubility of lycopene and the resistance to ions, acid, heat and other conditions, have good gastrointestinal stability and intestinal permeability, can effectively deliver lycopene to blood and liver and improve the bioavailability of lycopene, and can be applied to the fields of biological medicine, functional food and the like. In addition, the plastid pellets are derived from natural edible plant tissues, can meet the pursuit of consumers on health without side effects, and have wide development prospects.

To provide a method for delivering a nucleic acid molecule to the subpial space of a mammal to achieve spinal parenchymal infection thereof, and a system for spinal subpial gene delivery.SOLUTION: Provided herein is a method for delivering a nucleic acid molecule to the subpial space of a subject comprising the steps of: exposing a spinal segment of the vertebra of the subject; creating a pial opening within the spinal segment by puncturing the pia with an L-shaped stainless steel tube; advancing a catheter through the pial opening and into subpial space; and delivering the nucleic acid molecule to the subpial space of the subject, where the nucleic acid molecule is a vector or antisense oligonucleotide (ASO), and a system used in the method is also provided.SELECTED DRAWING: Figure 1A

To provide an alga belonging to Chlorophyta with an increased lipid content and improved lipid productivity.SOLUTION: Provided is a mutant alga derived from a parental strain belonging to Chlorophyta, having a plastid ATP / ADP transporter that comprises an amino acid partial sequence having at least 80% sequence identity to a specific amino acid sequence. Compared with the parental strain, the mutant alga exhibits reduced function of the plastid ATP / ADP transporter and increased lipid content and lipid productivity.SELECTED DRAWING: None

The invention relates to the technical field of medicines, and discloses an efficient external medicine for repairing leukoplakia vulvae and a preparation method of the efficient external medicine for repairing leukoplakia vulvae. 1.0 to 2.0 parts of vitamin B1; 0.5 to 1.5 parts of body ketone; 0.05 to 0.2 part of estradiol valerate; 1.0 to 5.0 parts of compound dexamethasone acetate cream; 0.5 to 2.0 parts of vitamin E; 2.0 to 5.0 parts of compound radix salviae miltiorrhizae extract; 1.0 to 3.0 parts of a red sensor ointment; 5.0 to 10.0 parts of plastid; 10.0 to 20.0 parts of a nano emulsion; and 15.0 to 25.0 parts of a temperature-sensitive gel matrix. Through nanocrystallization treatment, a multi-layer delivery system and a dynamic response release mechanism, the transdermal absorption efficiency and the delivery depth of the drug are remarkably improved; the temperature-sensitive gel and the acid and humidity-sensitive carrier are adopted, so that long-acting controlled release and adaptive release of a lesion environment of the medicine are realized; multi-component collaborative design comprehensively covers inflammation inhibition, fibrosis reversal and epithelium repair, and the treatment effect is improved.

This invention relates to methods of producing exogenous collagen in photosynthetic organelles and photosynthetic organisms, in particular methods of producing exogenous collagen in the plastid of an alga or plant, particularly in chloroplasts. Genetically altered algae and genetically altered plants, plant parts thereof or plant cells which express collagen in their chloroplasts are also included.

The invention discloses a genetic transformation method of erigeron breviscapus chloroplast. According to the method, plasmids containing target genes are introduced into explants prepared from erigeron breviscapus leaves through a gene gun method, and erigeron breviscapus transfection plants are obtained; wherein the parameters of the gene gun bombardment are as follows: the plasmid DNA concentration is 1-1.5 [mu] g / [mu] l, the number of bombardment times is 1-2, the target distance is 3-9 cm, the bombardment pressure is 900-1100 psi, and the vacuum degree is 26-28 mmHg. By adopting the method established by the invention, the homogenized erigeron breviscapus transfection plant can be efficiently obtained. The method has an important application value.

A method of expressing an agronomically or non-agronomically beneficial trait in a plant plastid comprising expressing an exogenous nucleic acid in the plant to produce non-photosynthetic mutant plants, and using callus grown from the mutant plants as recipients for introduction of a construct having a functional copy of the mutated gene and a gene conferring an agronomically or non-agronomically beneficial trait. Embodiments provide for mutations in chloroplast-encoded genes, as well as mutations in nuclear-encoded genes targeted to the chloroplast that are required for photosynthesis, plants and plant parts produced from such methods, as well as kits for performing the methods as described.

The invention discloses a gene and application related to the synthesis of tobacco plastid pigments. The gene related to the synthesis of tobacco plastid pigments is derived from tobacco and is named as pectin lyase (SPL), and its nucleotide sequence is shown in SEQ ID NO.1. The invention constructs a CRISPR / Cas9-spl editing vector for knocking out the pectin lyase gene by CRISPR / Cas9-mediated gene editing technology, obtains a safflower Dajinyuan plant with an insertion mutation in the pectin lyase gene after genetic transformation, and the content of neoxanthin, lutein, chlorophyll b and chlorophyll a in the leaves of the obtained edited plant 423 is significantly increased, indicating that the knockout of the gene can affect the content of plastid pigments in plant leaves, which provides genetic materials and theoretical basis for the study of tobacco plastid pigment synthesis pathways, tobacco leaf quality improvement, and tobacco variety genetic improvement.