The invention discloses a
grifola frondosa-derived
exosome-like nano-
vesicle as well as an extraction method and application thereof. The extraction method comprises the following steps:
cutting pilei and stipes of
grifola frondosa, and
grinding with a proper amount of cold PBS to prepare
grifola frondosa bacterial juice; the
grifola frondosa juice is centrifuged at 500 g-1, 000 g,
sediment is discarded, the obtained supernatant is centrifuged at 2,000 g-3,000 g,
sediment is discarded, the obtained supernatant is centrifuged at 10,000 g-12,000 g,
sediment is discarded, the obtained supernatant is filtered, and the pretreated
grifola frondosa juice is obtained; and centrifuging the pretreated
grifola frondosa juice at 30,000-100,000 g for 30 minutes to 4 hours, and resuspending by using PBS (
Phosphate Buffer Solution), so as to obtain the grifola frondosa-derived
exosome-like nano-vesicles. The invention develops a new thought for the mechanism of
cancer inhibition,
chemotherapy effect enhancement and
flora regulation of the grifola frondosa, and lays a foundation for application of the grifola frondosa in development of products such as related foods, functional foods, health foods, medicines and gel-loaded medicines.