265 results about "Lactoferrin" patented technology

The invention provides modified milk powder capable of promoting bone health as well as a preparation method and application of the modified milk powder. The total protein content of the modified milk powder is 17-35 g / 100 g, the fat content of the modified milk powder is 1-32 g / 100 g, and the calcium content of the modified milk powder is 0.8-2.1 g / 100 g. Moreover, the modified milk powder contains bovine coloctrum and lactoferrin, the mass ratio of the bovine coloctrum to the lactoferrin is (5-100): 1 on the basis of the dry matter content, and the content of the lactoferrin in the modified milk powder is 2-400 mg / 100 g. The bovine colostrum and the lactoferrin are combined for preparing the milk powder, have a synergistic effect on promoting osteoblasts and inhibiting osteoclast proliferation, and are beneficial to bone health.

The invention discloses a method for screening high-yield recombinant human lactoferrin through nucleic acid aptamer-assisted mutagenesis of a trichoderma reesei strain and application, and belongs to the technical field of microorganisms. The nucleic acid aptamer Lac-6a-FAM-BHQ1 capable of changing the fluorescence intensity according to the content of lactoferrin is constructed on the basis of the aptamer Lac-6a, and the nucleic acid aptamer can be used for rapidly selecting a mutant strain for producing high-titer recombinant human lactoferrin. The ARTP mutagenesis technology is combined with a nucleic acid aptamer auxiliary screening system, the mutant strain with the improved recombinant human lactoferrin yield is rapidly obtained from a large number of mutant strains, and the yield reaches 274.57 mg / L and is improved by 24.8% compared with that of an original strain. After the genes 111681 and 62716 are over-expressed in T. reesei, compared with a control strain, the yields of the recombinant human lactoferrin of the recombinant strain are respectively increased by 6.9% and 22.1%.

The invention discloses double-milk-source fermented milk of double-peak camel milk and brown milk and a preparation method of the double-milk-source fermented milk, and the preparation method comprises the following steps: mixing raw materials: mixing the double-peak camel milk and the brown milk according to a mass ratio to obtain double-milk-source mixed milk; pretreatment: sequentially carrying out homogenization treatment and heat treatment on the double-milk-source mixed milk; strain inoculation: inoculating the pretreated double-milk-source mixed milk with a fermentation strain, wherein the fermentation strain comprises a core strain and an auxiliary strain; the core strains are lactobacillus and streptococcus thermophilus which have tolerance to antibacterial components of the camel milk, and the core strains contain strains capable of producing exopolysaccharides. Through scientific matching of lactobacillus, streptococcus thermophilus and specific auxiliary strains which have tolerance to antibacterial components of the camel milk, the inhibition effect of natural antibacterial components such as lysozyme and lactoferrin in the camel milk on conventional strains is successfully overcome.

The invention discloses pichia pastoris for efficiently synthesizing lactoferrin as well as a construction method and application of the pichia pastoris, and belongs to the technical field of biosynthesis. The pichia pastoris for efficiently synthesizing the lactoferrin expresses at least one of KAR2, PDI1 and ERO1 in molecular chaperones in a host in an enhanced manner and carries an optimized secretory signal peptide, and the secretory signal peptide is obtained by replacing a leading peptide of alpha-factor with any signal peptide in LacZ, PelB, FLO and alpha-factor. The yield of LF can be effectively increased by enhancing expression of at least one of KAR2, PDI1 and ERO1 molecular chaperones in molecular chaperones in a host; when the lactoferrin expression cassette is integrated into the recombinant pichia pastoris X-33 genome and the copy number is increased to 2-6, the LF yield can be further increased, and when the copy number is 4, the LF yield reaches a peak value.

The invention belongs to the technical field of functional food, and particularly relates to a compound fermentation composition for regulating immunity and intestinal microecology as well as a preparation method and application of the compound fermentation composition. The invention provides a compound fermentation composition for regulating immunity and intestinal microecology. Comprising the following components: blueberry freeze-dried powder, an astragalus extract, a lycium ruthenicum extract, a day lily flower extract, grifola frondosa polysaccharide, schizochytrium limacinum powder, a red yeast rice extract, polygonatum polysaccharide, cordyceps militaris, xylooligosaccharide, yeast beta-glucan, lactoferrin, stevioside, a complex microbial inoculant and a complex enzyme. According to the compound fermentation composition disclosed by the invention, high-efficiency immunoregulation, long-acting intestinal balance and maximum component utilization are realized, and an efficient, stable and applicable solution is provided for regulating immunity and intestinal microecology.

A use of a nutritional composition in promoting the bidirectional development of the gut and brain via the gut-brain axis. The nutritional composition comprises the following essential components: docosahexaenoic acid, eicosatetraenoic acid, galactooligosaccharide, 1,3-dioleic acid-2-palmitic acid triglyceride, lutein, nucleotide, lactoferrin, casein phosphopeptide, and probiotics. By combining and strengthening multiple functional active components, the gut health and brain cognitive development of the organism can be bidirectionally promoted by means of the microbiota-gut-brain axis. In particular, the present application can promote the regulation of flora in the gut of the organism, increase the abundance of beneficial bacteria, and decrease the abundance of harmful bacteria related to brain development diseases. The content of butyric acid in the gut can be increased, and the contents of acetic acid and propionic acid are decreased to promote brain and cognitive development.

The invention belongs to the technical field of food processing, and particularly discloses a preparation process of natural preservative chicken soup based on lactobacillus fermentation. The process mainly comprises the following steps: decocting chicken and water according to a specific ratio to obtain basic chicken soup; the preparation method comprises the following steps: adding lactose, a yeast extract and the like as fermentation substrates, sterilizing, and inoculating a compound lactic acid bacteria starter consisting of lactobacillus plantarum and pediococcus pentosaceus to carry out anaerobic directional fermentation until the pH value is reduced to 4.0-4.6; and after fermentation is completed, adding natural preservative enhancers such as lactoferrin, and finally, homogenizing and filling to obtain the product. Natural preservation and flavor improvement of the chicken soup are synchronously achieved through a biological fermentation method, the obtained product is high in bacteriocin yield and remarkable in pathogenic bacterium inhibition effect, long-acting preservation can be achieved without adding chemical preservatives, meanwhile, the content of free amino acid and umami substances is greatly increased, and the fresh-keeping effect is good. The method is suitable for industrial production of high-quality concentrated chicken soup seasoning and liquid soup base.

The present application relates to a nutritional composition, a product comprising the same and use thereof in the preparation of a product for alleviating synaptic plasticity impairment of a body. The nutritional composition comprises: docosahexaenoic acid (DHA) and / or a metabolic precursor thereof, sodium hyaluronate (SH), and lactoferrin (LF). When docosahexaenoic acid (DHA), sodium hyaluronate (SH) and lactoferrin (LF) are used in combination, they can synergistically alleviate synaptic plasticity impairment of a body, in particular, increase nerve growth factor (NGF) content and / or brain-derived neurotrophic factor (BDNF) content in PC12 cells of the body, and / or increase expression of synaptic-related proteins in PC12 cells of the body.

This invention relates to the field of pet care product technology, and discloses a pet oral care spray and its preparation method. The preparation method employs a low-temperature gradient stepwise dissolution process: glucose oxidase is pre-dissolved at low temperature to obtain pre-solution A; bovine colostrum powder and lactoferrin are dissolved at low temperature to obtain protein pre-solution B; honey, folic acid, and vitamin D3 are dissolved at room temperature to obtain excipient pre-solution C; lysozyme is added to protein pre-solution B and stirred to dissolve; then pre-solution A and excipient pre-solution C are combined sequentially, the pH is adjusted, and purified water is added to make up the volume; the mixture is filtered through a microporous membrane for sterilization, and then filled and sealed under inert gas protection. This preparation method effectively protects the biological activity of each active protein and enzyme, achieving a long shelf life without the need for additional chemical preservatives. The resulting spray does not contain irritating ingredients such as alcohol and menthol, and is suitable for improving stomatitis, inhibiting plaque and tartar, and alleviating halitosis in pets of all ages and breeds.

The invention relates to the field of food, in particular to a yeast beta-glucan composition for preventing and relieving upper respiratory tract infection. The composition provided by the invention mainly takes yeast beta-glucan and lactoferrin as main raw materials, and comprises the following components in percentage by weight: 0.1 to 80 percent of yeast beta-glucan and 0.1 to 80 percent of lactoferrin. The composition provided by the invention can also be prepared from other selective synergistic components and auxiliary materials through a series of processing technologies. The composition provided by the invention can ensure the complete structure and activity of the effective components, and has the effects of boosting immunity and preventing and relieving upper respiratory tract infection.

The invention belongs to the technical field of microorganisms, and particularly relates to a method for expressing human lactoferrin by using lactococcus lactis. The method comprises the following steps: step 1, obtaining a codon-optimized hLF gene nucleotide sequence; 2, constructing a recombinant plasmid containing the hLF gene nucleotide sequence obtained in the step 1; and step 3, transferring the recombinant plasmid obtained in the step 2 into lactococcus lactis for induced expression. The heterologous expression of the human lactoferrin hLF is realized by utilizing the lactococcus lactis, the method is low in cost, convenient and fast, and a new way is provided for high-purity and high-yield preparation of the human lactoferrin hLF.

This application discloses a sheep milk probiotic formula milk powder that can regulate intestinal flora and its preparation method. By weight, it contains the following raw material components: 600-700 parts sheep milk base, 5-15 parts compound probiotic powder, 30-60 parts prebiotic composition, 10-30 parts functional additives, and 5-20 parts nutritional fortifiers. This compound formula combines multiple probiotics and optimizes the strain ratio to achieve a synergistic colonization effect. The probiotics are prepared using a freeze-drying process, effectively improving problems caused by flora imbalance. Simultaneously, multiple compound prebiotics provide nutritional support for the proliferation of beneficial bacteria, synergistically enhancing the intestinal regulatory effect. Added functional components such as lactoferrin, chitosan, β-glucan, and poria cocos polysaccharide make it suitable for long-term conditioning consumption by sub-healthy individuals and those with weak digestive systems. The raw sheep milk undergoes vacuum distillation to remove the muttony odor. A process of wet powdering followed by aseptic dry mixing of probiotics is used, resulting in a more stable overall quality of the finished product.

The invention relates to human lactoferrin for use in enhancing bone strength and / or bone growth in a subject.

The invention discloses a lactoferrin gel carrier, a preparation method thereof and application of the lactoferrin gel carrier in an oral delivery system. The preparation method comprises the following steps: mixing a lactoferrin solution with transglutaminase, and reacting under mild conditions to form a gel network. According to the method, gel which is compact in structure and high in stability is formed through enzyme catalytic crosslinking, the mechanical strength and embedding capacity of the gel are remarkably enhanced, and the method is suitable for embedding multiple active ingredients (such as probiotics, polyphenol and vitamins) sensitive to heat, oxygen or shear force. The obtained gel carrier can effectively isolate oxygen, moisture and adverse processing environments, and the stability of embedded components in processing, storage and transportation is remarkably improved. The carrier can be slowly released in a gastrointestinal tract environment, and active ingredients are prevented from being degraded too early in gastric acid, so that the intestinal absorption rate and the bioavailability of the carrier are improved. The materials used in the invention are natural proteins, are safe and non-toxic, and accord with food and health care product application standards.

The invention discloses a pet food containing probiotic-lactoferrin pellets and a preparation method of the pet food. An immune golden triangle formula is adopted, layered wrapping is carried out, active probiotics, prebiotics, protein for enhancing immunity and antioxidant substances are used as formula raw materials, the probiotics are wrapped by the prebiotics and milk fat globule membrane protein, and then the prebiotics and the milk fat globule membrane protein are wrapped by lactoferrin and shiitake mushroom powder. And finally, coating lactoferrin with a coating solution containing antioxidant substances to prepare pellets, and applying the pellets to preparation of pet food. Functional substances such as probiotics and immune protein are delivered to the intestinal tract through multi-layer wrapping, and various functional components capable of improving immunity in the intestinal tract have a synergistic effect, so that the immunity of pets can be remarkably improved.

The invention discloses a nutritional composition for short-time internal inflammation balance after medium-high-strength resistance training and application of the nutritional composition, and belongs to the technical field of food processing. The composition comprises an inflammation control component composed of a plant inflammation control component and a milk source inflammation control component, the inflammation control component is composed of a plant source component and a milk source component and specifically comprises omega-3 unsaturated fatty acid, curcumin, iron unsaturated lactoferrin and a milk fat globule membrane according to a specific weight ratio, the weight ratio of EPA to DHA in the omega-3 unsaturated fatty acid is not lower than 1.5: 1, and the weight ratio of the curcumin to the iron unsaturated lactoferrin to the milk fat globule membrane is not lower than 1.5: 1. The iron saturation of the iron unsaturated lactoferrin is less than or equal to 30%, and the phospholipid accounts for 4-10% of the milk fat ball membrane. Experimental results show that the composition can effectively regulate and control the level of inflammatory factors within 6 hours after training, and accurate regulation and control of inflammatory response within an inflammatory regulation window period within 6 hours after training are achieved. The invention also relates to application of the composition in preparation of functional food and dairy products.

The application belongs to the field of food functional factor processing, and particularly relates to a water-in-oil-in-water composite emulsion embedding lactoferrin, a preparation method and application. The W1 / O / W2 double emulsion system is constructed by a two-step method, and the leakage and inactivation risks of lactoferrin and VD3 in the preparation, storage and processing processes are effectively reduced through the double barrier effect of the oil phase film and the outer water phase film. Under the optimized sodium caseinate and gellan gum compounding conditions, the encapsulation rate of lactoferrin can be up to 82.52%, which proves that the structure has obvious advantages in improving the loading efficiency of active substances, and can significantly improve the encapsulation rate and structural stability of lactoferrin. The water-in-oil-in-water composite emulsion system prepared by the application can significantly improve the retention rate of lactoferrin in the digestion process, enhance the tolerance of lactoferrin to the gastrointestinal environment, and then enhance the arrival rate of lactoferrin in the intestinal tract, thereby improving the overall bioavailability.

The application discloses a multi-effect repairing and anti-allergy toothpaste composition and a preparation method thereof. The toothpaste comprises the following raw material components in percentage by mass: hydroxyapatite 1.0-10.0%, zeolite 0.02-0.8%, vitamin E 0.02-0.5%, lactoferrin 0.0005-0.02%, and the rest is base. When harmful bacteria form a biofilm in the oral cavity, the zeolite can destroy the biofilm formed by the harmful bacteria, so that the vitamin E and the lactoferrin enter the biofilm. The lactoferrin adsorbs iron ions near the harmful bacteria, so that the harmful bacteria cannot form a biofilm again. After rinsing, a large amount of water can take away the harmful bacteria, and finally the hydroxyapatite repairs. The effective synergistic closed loop of the hydroxyapatite, the zeolite, the vitamin E and the lactoferrin reconstructs a healthy oral micro-ecology from the source.

The present application relates to the field of biosynthesis and microbial fermentation engineering technology, in particular to a kind of secretion expression human lactoferrin Yarrowia lipolytica and its construction method and application.The present application provides a kind of secretion expression human lactoferrin recombinant Yarrowia lipolytica, the secretion expression of human lactoferrin in the recombinant Yarrowia lipolytica is guided by secretion signal peptide;The expression of chaperone HAC1, Cne and Pdi1 is simultaneously enhanced.The present application realizes the secretion expression of human lactoferrin in Yarrowia lipolytica, effectively promotes the folding and secretion expression of human lactoferrin by the cooperation of chaperone, significantly improves the yield of human lactoferrin, and has important application value in the industrial scale production of human lactoferrin.

This invention discloses a method and application for efficient synthesis of human lactoferrin through multi-copy integration, relating to the fields of genetic engineering and bioengineering. The invention provides a method for synthesizing human lactoferrin, including the steps of fermenting and culturing a recombinant Aspergillus niger strain, followed by isolation and purification to obtain human lactoferrin; the construction method of the recombinant Aspergillus niger strain includes the step of integrating the hLF gene into a gene locus in the host strain genome to construct the recombinant Aspergillus niger strain. This invention constructs a recombinant Aspergillus niger strain capable of recombinantly expressing human lactoferrin, initiating protein expression through a strongly inducible promoter, fusing endogenous high-secretion proteins, and optimizing the multi-copy integration method to integrate the lactoferrin expression cassette, achieving high-level synthesis of human lactoferrin, laying the foundation for the industrial production of lactoferrin or high-density fermentation of other food functional proteins using Aspergillus niger.

The invention discloses a si-TGM2-loaded macrophage targeting nano-material as well as a preparation method and application thereof, relates to the technical field of biological targeting, and aims at solving the problem that a macrophage targeting nano-drug for inhibiting severe acute pancreatitis aiming at a TGM2 target is lacked in the prior art. The technical key points of the invention are as follows: the si-TGM2-loaded macrophage targeting nano material is provided, the si-TGM2-loaded macrophage targeting nano material is a lactoferrin modified cationic lipid nucleic acid drug, and the cationic lipid nucleic acid drug is prepared by coating si-TGM2 as shown in SEQ.ID. NO.1 with a cationic liposome. The si-TGM2-loaded macrophage targeting nanometer material disclosed by the invention has a wide application prospect in preparation of an acute pancreatitis diagnostic kit and a medicine.

Provided are use of a bioactive protein in the preparation of a drug for treating herpes virus infection and a pharmaceutical composition for treating herpes virus infection. The pharmaceutical composition mainly comprises a bioactive protein, a matrix, a humectant, a pH regulator, and a preservative. The present invention has the following technical effects: 1) By means of a large amount of research and experimental verification, the inventors have discovered for the first time that anhydridized lactoferrin, a modified bioactive protein, has an inhibitory effect on herpes zoster virus (VZV). 2) In the pharmaceutical composition, the type and amount of excipient and the proportion of the main ingredient are reasonable. The pharmaceutical composition is administered in ointment form, in spray form, or in other dosage forms suitable for external use, has a significant therapeutic effect, has small local toxic side effects, has very low acute dermal toxicity, causes no irritation or allergy, does not interfere with normal skin functions, and is safe and effective.

The invention provides an oral care composition for inhibiting helicobacter pylori and a preparation method of the oral care composition. The oral care composition comprises glycerin, xylitol, erythritol, thymol clathrate, garlic extract clathrate, propolis extract, lactoferrin, disodium ethylene diamine tetraacetate, menthol, potassium sorbate and purified water. The thymol clathrate compound is obtained by carrying out clathration on thymol through beta-cyclodextrin. The garlic extract clathrate compound is obtained by clathrating a garlic extract with beta-cyclodextrin. According to the oral care composition provided by the invention, the helicobacter pylori in the oral cavity can be effectively inhibited through reasonable proportioning of various components, so that the risks of ozostomia and stomach reinfection are reduced.

This invention discloses a camel milk formula powder for assisting in the regulation of blood lipids and its preparation process, belonging to the field of functional dairy product deep processing technology. This formula powder constructs a five-in-one synergistic lipid-regulating network of "exogenous absorption inhibition, endogenous synthesis regulation, oxidative stress protection, intestinal microecological regulation, and vascular endothelial protection." Its core components include a specific ratio of compounded phytosterol esters, microencapsulated complex oils, and medicinal and edible extracts. The preparation process employs stepwise low-temperature microencapsulation and cold spray agglomeration granulation technology to achieve full-process low-temperature protection of active ingredients, with lactoferrin retention ≥90% and ω-3 fatty acid bioavailability increased by more than 25%. This invention's product has comprehensive lipid-regulating effects, 3 times improved oxidative stability, and produces no oil residue or off-odor when mixed, making it suitable for long-term consumption by people with borderline elevated blood lipids. It can also achieve continuous industrial production with a batch mixing uniformity RSD ≤2%.

The application provides a high-stability DHA emulsion and a preparation method thereof. The high-stability DHA emulsion comprises the following raw materials in parts by mass: 20-40 parts of DHA algal oil; 1-3 parts of phospholipid; 2-5 parts of emulsifier; 0.05-0.3 parts of fat-soluble antioxidant; 0.05-0.3 parts of water-soluble antioxidant; 2-3 parts of lactoferrin-reducing oligosaccharide conjugate; 0.5-2 parts of polysaccharide; and 55-75 parts of water. The high-stability DHA emulsion can be used as a nutritional fortifier for milk and dairy products, beverages and formula food.

A nutritional composition for an Alzheimer's disease population or a high-risk population, prepared from the following raw materials in parts by weight: 170-300 parts of proteinaceous substances, 85-250 parts of fatty substances, 450-770 parts of carbohydrates, 10-155 parts of dietary fiber substances, 1.2-3.5 parts of trace minerals, 47-180 parts of macro minerals, 2.0-8.3 parts of vitamins, 0.1-1.0 parts of taurine, 0.05-0.5 parts of L-carnitine, and 0.9-1.0 parts of lactoferrin. The composition is rich in high-quality animal and plant proteins, fatty acids, various vitamins, minerals, and water-soluble dietary fibers, has remarkable effects in slowing down the occurrence and development of Alzheimer's disease, and is suitable for an Alzheimer's disease population or a high-risk population to eat.

This invention discloses a kitten food without palatability enhancers and its preparation method, relating to the field of pet food technology. It comprises the following components: polypeptide chicken meal, soy protein peptides, gluten hydrolysate, lactoferrin, astragalus extract, yam powder, rosemary oil, resveratrol, tributyrate, and rice. By adding substances containing small peptides and plant extracts to the kitten food, this invention provides the body with a large amount of high-quality animal protein without adding any chemically synthesized palatability enhancers, preservatives, or other chemical additives. This effectively promotes the body's decomposition and metabolism of nutrients, promotes the growth of kittens, and also enhances the body's antioxidant capacity and reduces the occurrence of diarrhea.

This invention belongs to the field of cosmetic technology, specifically relating to an anti-aging composition containing lactoferrin and its application. The anti-aging composition provided by this invention, through scientific compounding, achieves synergistic effects in anti-aging among four components: medicinal Fomitopsis thunbergii extract, butterfly pea flower extract, Artemisia annua extract, and lactoferrin. This results in excellent elastase inhibition, preventing the degradation of elastin in the dermis and effectively strengthening the elastin scaffold, thereby enhancing the anti-aging effect of the composition. When applied to a prepared emulsion, this anti-aging composition can improve skin firmness (R0 change rate), skin elasticity (R2 change rate), and facial wrinkles (wrinkle change rate), exhibiting anti-aging effects of improving wrinkle reduction and firmness, and promoting skin elasticity. Furthermore, it does not cause skin irritation, possessing safe and gentle characteristics, a wide range of applications, and high consumer acceptance.

The invention belongs to the technical field of food processing and biological medicines, and relates to a ginsenoside Rb1-lactoferrin composite emulsion with an anti-inflammatory effect and a preparation method thereof. The emulsion is prepared from the following components in parts by weight: 0.1 part of ginsenoside Rb1-lactoferrin complex, 7.5 to 8.5 parts of peanut oil and 1.4 to 2.4 parts of water. The emulsion disclosed by the invention has better stability, bioaccessibility and anti-inflammatory activity, does not have unstable phenomena such as layering and the like after being stored at room temperature for 35 days, and has a wide application prospect in food and pharmaceutical industries.

The invention provides a biotinylated Angiopep-2 modified targeting nano material as well as a preparation method and application of the biotinylated Angiopep-2 modified targeting nano material. According to the preparation method of the biotinylated Angiopep-2 modified targeting nano-material, Angiopep-2 is directionally anchored through a high-affinity biotin-streptavidin system, ligand conformation inactivation caused by chemical coupling is avoided, targeting activity is maintained, the used Angiopep-2 can help nanoparticles to be specifically recognized and combined and mediate the nanoparticles to cross a blood brain barrier to enter the brain, and the targeting nano-material can be used for preparing the targeted nano-material. The drug concentration in brain tissues is increased, so that the cure rate of brain diseases is improved, the brain targeting efficiency is far higher than that of transferrin and lactoferrin, and the application value is great; by entrapping curcumin and quercetin, a hydrophilic shell is provided, so that the water solubility of curcumin and quercetin is improved, and the bioavailability of curcumin and quercetin is improved.