355 results about "Lactoferrin" patented technology

The present invention belongs to the field of biomedicine, and specifically relates to an antimicrobial peptide derived from lactoferrin and its application. The antimicrobial peptide derived from lactoferrin, whose amino acid sequence is shown in any one of SEQ ID NOs: 1 to 98, has a broad-spectrum antimicrobial effect, especially significant antibacterial effect against Pseudomonas putida, Rhodococcus erythropolis, Bacillus subtilis, Malassezia furfur, Cryptococcus neoformans, and Candida albicans, and has great application value.

The invention discloses an application of knockout of PEP4 and BAR1 genes in reduction of degradation of Kluyveromyces marxianus on lactoferrin. The invention provides application of inhibiting expression of PEP4 protein and BAR1 protein in any one of the following aspects: (A1) reducing degradation of Kluyveromyces marxianus on lactoferrin; and (A2) reducing the degradation of the fermentation supernatant of the kluyveromyces marxianus on lactoferrin. Experiments prove that after the encoding gene of the PEP4 protein and the encoding gene of the BAR1 protein are knocked out in yeast cells, the lactoferrin degradation amount can be reduced, the complete protein amount is remarkably increased, and the method has important significance on increasing the secretory expression yield of the lactoferrin in kluyveromyces marxianus.

The invention provides a milk powder composition containing breast milk oligosaccharide and easy to digest and absorb. The milk powder composition comprises the breast milk oligosaccharide, OPN, galactooligosaccharide, lactoferrin, alpha-whey protein, multivitamins, lactose, vegetable oil and compound minerals, wherein the weight ratio of the lactoferrin to the OPN to the breast milk oligosaccharide to the galactooligosaccharide is 1: (2-4): (2-50): (4-100). The composition can effectively recover and improve the intestinal barrier function caused by inflammatory factors, can be well absorbed by infants with poor digestion and absorption functions, reduces diarrhea and promotes skeletal development.

The invention belongs to the technical field of medical nano-materials, and particularly relates to a lactoferrin nano-material for sonodynamic regulation and control of blood-brain barrier permeability as well as a preparation method and application of the lactoferrin nano-material. The nanomaterial is nanoparticles LCe6 formed by self-assembly of lactoferrin and chlorin e6, can target brain microvascular endothelial cells, and can temporarily, reversibly and targeting open BBB by using the sonodynamic effect of the LCe6 in combination with transcranial ultrasonic stimulation, so that the accumulation of the nanoparticles LCe6 at a focus part is improved; in addition, the nanoparticle LCe6 also has a targeting property on brain tumor cells, the tumor cells can be accurately killed by utilizing the sonodynamic effect of the nanoparticle under secondary transcranial ultrasound, and the effect of sonodynamic treatment on brain glioma is exerted to the maximum extent; the nanoparticles LCe6 have excellent biocompatibility and low toxicity; according to the invention, the intracerebral delivery efficiency of the drug can be obviously improved, and a novel method for targeting open brain disease BBB is provided.

The invention relates to the technical field of food processing, in particular to antioxidant nano collagen peptide powder and a preparation method thereof.The antioxidant nano collagen peptide powder is prepared by forming antioxidant micelles wrapping collagen peptide through collagen peptide liquid and a complex antioxidant, loading the antioxidant micelles on a porous carrier and then conducting cross-linking through transglutaminase; tea polyphenol and whey protein are covalently combined to prepare a composite antioxidant, rapid free radical neutralization, lactoferrin inhibition of an oxidation source and synergistic effect of collagen peptide self-antioxidant groups are provided, multistage barrier design is combined, oxygen diffusion is isolated through a micelle hydrophobic core, and the contact area of micelles and the outside is reduced through physical adsorption of a porous carrier, so that the anti-oxidation effect of the collagen peptide is improved. Finally, the micelles are fixed in the carrier by utilizing an enzyme cross-linked network, so that the antioxidant nano collagen peptide powder has efficient and long-acting antioxidant capacity.

The invention provides modified milk powder capable of promoting bone health as well as a preparation method and application of the modified milk powder. The total protein content of the modified milk powder is 17-35 g / 100 g, the fat content of the modified milk powder is 1-32 g / 100 g, and the calcium content of the modified milk powder is 0.8-2.1 g / 100 g. Moreover, the modified milk powder contains bovine coloctrum and lactoferrin, the mass ratio of the bovine coloctrum to the lactoferrin is (5-100): 1 on the basis of the dry matter content, and the content of the lactoferrin in the modified milk powder is 2-400 mg / 100 g. The bovine colostrum and the lactoferrin are combined for preparing the milk powder, have a synergistic effect on promoting osteoblasts and inhibiting osteoclast proliferation, and are beneficial to bone health.

The invention discloses a method for screening high-yield recombinant human lactoferrin through nucleic acid aptamer-assisted mutagenesis of a trichoderma reesei strain and application, and belongs to the technical field of microorganisms. The nucleic acid aptamer Lac-6a-FAM-BHQ1 capable of changing the fluorescence intensity according to the content of lactoferrin is constructed on the basis of the aptamer Lac-6a, and the nucleic acid aptamer can be used for rapidly selecting a mutant strain for producing high-titer recombinant human lactoferrin. The ARTP mutagenesis technology is combined with a nucleic acid aptamer auxiliary screening system, the mutant strain with the improved recombinant human lactoferrin yield is rapidly obtained from a large number of mutant strains, and the yield reaches 274.57 mg / L and is improved by 24.8% compared with that of an original strain. After the genes 111681 and 62716 are over-expressed in T. reesei, compared with a control strain, the yields of the recombinant human lactoferrin of the recombinant strain are respectively increased by 6.9% and 22.1%.

The invention belongs to the technical field of biological medicines, and particularly relates to application of bifidobacterium breve and lactoferrin in preparation of products for regulating intestinal flora or resisting bacterial infection. The invention provides application of Bifidobacterium breve HH079 with the preservation number of GDMCC No: 64216 and lactoferrin in preparation of a product for regulating intestinal flora or a product for resisting bacterial infection, and the Bifidobacterium breve HH079 and the lactoferrin are combined and synergistically used for resisting the bacterial infection. The composition can reverse weight loss, colon shortening, bacterial load increase and colon epithelium injury caused by bacterial infection, can significantly inhibit the level of proinflammatory cytokines, promote the expression of anti-inflammatory cytokines, can significantly increase the concentration of acetic acid and propionic acid in excrement of infected mice, can generate positive regulation on intestinal flora, and can inhibit bacterial infection. Good application prospects are shown in regulation of intestinal flora and bacterial infection resistance.

The invention relates to the technical field of pet food, in particular to flavoring agent powder for pet dogs and a preparation method of the flavoring agent powder. Comprising the following raw materials: 40 parts of beef liver, 10 parts of hairtail, 7-40 parts of ricefield eel, 1 part of protease, 0.5-1 part of lipase, 0.5-1 part of flavor enzyme, 5-10 parts of saccharides, 10 parts of fish oil, 5-10 parts of maltodextrin, 0.5-1 part of sodium hydroxide, 5 parts of phosphoric acid, 3 parts of amino acid, 2 parts of thiamine, 1 part of yeast extract and 0.5-1 part of lactoferrin. The dog feed can increase the appetite of dogs from the sense of smell, maintain the health balance of intestinal flora of dogs and improve the micro-ecological environment of intestinal tracts.

The invention discloses sheep colostrum immune globulin powder and a preparation process thereof, and relates to the technical field of protein powder. The sheep colostrum immune globulin powder is prepared from the following components in parts by weight: 200 to 250 parts of a sheep colostrum raw material, 350 to 400 parts of whole milk powder, 330 to 370 parts of skimmed milk powder, 30 to 50 parts of prebiotics, 8 to 12 parts of acerola cherry powder, 0.8 to 1.2 parts of lactoferrin, 0.4 to 0.6 part of N-acetylneuraminic acid and 0.3 to 0.5 part of probiotics. Diversified nutritional requirements of a human body are fully considered, various nutritional ingredients are matched with one another and have a synergistic effect, the nutritional ingredients can be better absorbed and utilized by the human body, comprehensive nutritional support is provided for consumers, and the requirements of daily nutritional supplement, physique enhancement, immunity improvement and the like are met.

The invention discloses double-milk-source fermented milk of double-peak camel milk and brown milk and a preparation method of the double-milk-source fermented milk, and the preparation method comprises the following steps: mixing raw materials: mixing the double-peak camel milk and the brown milk according to a mass ratio to obtain double-milk-source mixed milk; pretreatment: sequentially carrying out homogenization treatment and heat treatment on the double-milk-source mixed milk; strain inoculation: inoculating the pretreated double-milk-source mixed milk with a fermentation strain, wherein the fermentation strain comprises a core strain and an auxiliary strain; the core strains are lactobacillus and streptococcus thermophilus which have tolerance to antibacterial components of the camel milk, and the core strains contain strains capable of producing exopolysaccharides. Through scientific matching of lactobacillus, streptococcus thermophilus and specific auxiliary strains which have tolerance to antibacterial components of the camel milk, the inhibition effect of natural antibacterial components such as lysozyme and lactoferrin in the camel milk on conventional strains is successfully overcome.

The invention discloses a resveratrol delivery system based on lactoferrin-polyglutamic acid composite nanogel and a preparation method thereof.The preparation method comprises the steps that a lactoferrin aqueous solution and a gamma-sodium polyglutamate aqueous solution are mixed to form a composite system, resveratrol is embedded with the composite system, and the resveratrol delivery system is obtained. And finally, adding calcium chloride into the resveratrol-loaded lactoferrin / polyglutamic acid mixed system, so that the calcium chloride and calcium ions are subjected to ionic crosslinking to form stable nanogel, and freeze-drying the nanogel to obtain the high-resveratrol nanogel powder. According to the invention, lactoferrin and polyglutamic acid are selected to be compounded to construct a resveratrol-loaded lactoferrin / polyglutamic acid nanogel system, a composite structure is formed by utilizing electrostatic interaction between protein and peptide polyacid, and then the composite structure is crosslinked with Ca < 2 + > to form a stable core-shell type three-dimensional nanogel structure. The water solubility, the drug loading capacity and the biological accessibility of the resveratrol are obviously improved.

The invention discloses pichia pastoris for efficiently synthesizing lactoferrin as well as a construction method and application of the pichia pastoris, and belongs to the technical field of biosynthesis. The pichia pastoris for efficiently synthesizing the lactoferrin expresses at least one of KAR2, PDI1 and ERO1 in molecular chaperones in a host in an enhanced manner and carries an optimized secretory signal peptide, and the secretory signal peptide is obtained by replacing a leading peptide of alpha-factor with any signal peptide in LacZ, PelB, FLO and alpha-factor. The yield of LF can be effectively increased by enhancing expression of at least one of KAR2, PDI1 and ERO1 molecular chaperones in molecular chaperones in a host; when the lactoferrin expression cassette is integrated into the recombinant pichia pastoris X-33 genome and the copy number is increased to 2-6, the LF yield can be further increased, and when the copy number is 4, the LF yield reaches a peak value.

The invention provides freeze-dried pet food capable of improving intestinal functions of pets and a preparation method of the freeze-dried pet food. Firstly, lecithin and beta-sitosterol are used for constructing lipidosome embedded compound bacteria, and then lactoferrin is combined with embedded compound enzyme to form microspheres; chitosan oligosaccharide is used for connecting compound bacteria liposome and compound enzyme microspheres, the chitosan oligosaccharide is loaded in an alginate gel network to prepare a compound microcapsule, finally raw materials such as yoghourt, camel milk, milk mineral salt and galactooligosaccharide are mixed, and the freeze-dried pet food is prepared through vacuum freeze drying. The sodium alginate composite microcapsule prepared by the invention can greatly improve the stability of probiotics and enzymes, ensure the activity of the probiotics and the enzymes, and improve the palatability of pets to daily ration, increase the food consumption of the pets, promote the digestion and absorption of nutrient substances by the intestinal tracts of the pets and improve the immunity of the pets by virtue of targeted release of the intestinal tracts and synergistic effect of the prebiotics. The intestinal function of the pet is improved, and an efficient and safe solution is provided for the intestinal health of the pet.

The invention discloses a composition based on the synergistic effect of an SOD-LF covalent conjugate and neuromodulation and application of the composition in preparation of drugs for relieving anxiety and insomnia and improving sleep quality, and belongs to the technical field of biology. The preparation method comprises the following steps: preparing an SOD-LF covalent conjugate from lactoferrin and SOD; uniformly dispersing the SOD-LF covalent conjugate in a sodium alginate solution, spraying into a calcium chloride solution, standing, and filtering to obtain a sodium alginate coated SOD-LF covalent conjugate; and dispersing the sodium alginate coated SOD-LF covalent conjugate and fucoidin in a chitosan solution, adding sodium tripolyphosphate, stirring for reaction, and centrifugally collecting to obtain the composition based on the synergistic effect of the SOD-LF covalent conjugate and nerve regulation. According to the composition disclosed by the invention, the blood brain barrier transmittance is enhanced through the SOD-LF covalent conjugate, and the fucoidin is combined to regulate the intestinal flora-enteric brain axis to synergistically inhibit neuroinflammation and oxidative stress, so that anxiety and insomnia are improved.

The invention belongs to the technical field of functional food, and particularly relates to a compound fermentation composition for regulating immunity and intestinal microecology as well as a preparation method and application of the compound fermentation composition. The invention provides a compound fermentation composition for regulating immunity and intestinal microecology. Comprising the following components: blueberry freeze-dried powder, an astragalus extract, a lycium ruthenicum extract, a day lily flower extract, grifola frondosa polysaccharide, schizochytrium limacinum powder, a red yeast rice extract, polygonatum polysaccharide, cordyceps militaris, xylooligosaccharide, yeast beta-glucan, lactoferrin, stevioside, a complex microbial inoculant and a complex enzyme. According to the compound fermentation composition disclosed by the invention, high-efficiency immunoregulation, long-acting intestinal balance and maximum component utilization are realized, and an efficient, stable and applicable solution is provided for regulating immunity and intestinal microecology.

A use of a nutritional composition in promoting the bidirectional development of the gut and brain via the gut-brain axis. The nutritional composition comprises the following essential components: docosahexaenoic acid, eicosatetraenoic acid, galactooligosaccharide, 1,3-dioleic acid-2-palmitic acid triglyceride, lutein, nucleotide, lactoferrin, casein phosphopeptide, and probiotics. By combining and strengthening multiple functional active components, the gut health and brain cognitive development of the organism can be bidirectionally promoted by means of the microbiota-gut-brain axis. In particular, the present application can promote the regulation of flora in the gut of the organism, increase the abundance of beneficial bacteria, and decrease the abundance of harmful bacteria related to brain development diseases. The content of butyric acid in the gut can be increased, and the contents of acetic acid and propionic acid are decreased to promote brain and cognitive development.

The invention belongs to the technical field of food processing, and particularly discloses a preparation process of natural preservative chicken soup based on lactobacillus fermentation. The process mainly comprises the following steps: decocting chicken and water according to a specific ratio to obtain basic chicken soup; the preparation method comprises the following steps: adding lactose, a yeast extract and the like as fermentation substrates, sterilizing, and inoculating a compound lactic acid bacteria starter consisting of lactobacillus plantarum and pediococcus pentosaceus to carry out anaerobic directional fermentation until the pH value is reduced to 4.0-4.6; and after fermentation is completed, adding natural preservative enhancers such as lactoferrin, and finally, homogenizing and filling to obtain the product. Natural preservation and flavor improvement of the chicken soup are synchronously achieved through a biological fermentation method, the obtained product is high in bacteriocin yield and remarkable in pathogenic bacterium inhibition effect, long-acting preservation can be achieved without adding chemical preservatives, meanwhile, the content of free amino acid and umami substances is greatly increased, and the fresh-keeping effect is good. The method is suitable for industrial production of high-quality concentrated chicken soup seasoning and liquid soup base.

The present application relates to a nutritional composition, a product comprising the same and use thereof in the preparation of a product for alleviating synaptic plasticity impairment of a body. The nutritional composition comprises: docosahexaenoic acid (DHA) and / or a metabolic precursor thereof, sodium hyaluronate (SH), and lactoferrin (LF). When docosahexaenoic acid (DHA), sodium hyaluronate (SH) and lactoferrin (LF) are used in combination, they can synergistically alleviate synaptic plasticity impairment of a body, in particular, increase nerve growth factor (NGF) content and / or brain-derived neurotrophic factor (BDNF) content in PC12 cells of the body, and / or increase expression of synaptic-related proteins in PC12 cells of the body.

This invention relates to the field of pet care product technology, and discloses a pet oral care spray and its preparation method. The preparation method employs a low-temperature gradient stepwise dissolution process: glucose oxidase is pre-dissolved at low temperature to obtain pre-solution A; bovine colostrum powder and lactoferrin are dissolved at low temperature to obtain protein pre-solution B; honey, folic acid, and vitamin D3 are dissolved at room temperature to obtain excipient pre-solution C; lysozyme is added to protein pre-solution B and stirred to dissolve; then pre-solution A and excipient pre-solution C are combined sequentially, the pH is adjusted, and purified water is added to make up the volume; the mixture is filtered through a microporous membrane for sterilization, and then filled and sealed under inert gas protection. This preparation method effectively protects the biological activity of each active protein and enzyme, achieving a long shelf life without the need for additional chemical preservatives. The resulting spray does not contain irritating ingredients such as alcohol and menthol, and is suitable for improving stomatitis, inhibiting plaque and tartar, and alleviating halitosis in pets of all ages and breeds.

The invention relates to the field of food, in particular to a yeast beta-glucan composition for preventing and relieving upper respiratory tract infection. The composition provided by the invention mainly takes yeast beta-glucan and lactoferrin as main raw materials, and comprises the following components in percentage by weight: 0.1 to 80 percent of yeast beta-glucan and 0.1 to 80 percent of lactoferrin. The composition provided by the invention can also be prepared from other selective synergistic components and auxiliary materials through a series of processing technologies. The composition provided by the invention can ensure the complete structure and activity of the effective components, and has the effects of boosting immunity and preventing and relieving upper respiratory tract infection.

The invention belongs to the technical field of microorganisms, and particularly relates to a method for expressing human lactoferrin by using lactococcus lactis. The method comprises the following steps: step 1, obtaining a codon-optimized hLF gene nucleotide sequence; 2, constructing a recombinant plasmid containing the hLF gene nucleotide sequence obtained in the step 1; and step 3, transferring the recombinant plasmid obtained in the step 2 into lactococcus lactis for induced expression. The heterologous expression of the human lactoferrin hLF is realized by utilizing the lactococcus lactis, the method is low in cost, convenient and fast, and a new way is provided for high-purity and high-yield preparation of the human lactoferrin hLF.

This application discloses a sheep milk probiotic formula milk powder that can regulate intestinal flora and its preparation method. By weight, it contains the following raw material components: 600-700 parts sheep milk base, 5-15 parts compound probiotic powder, 30-60 parts prebiotic composition, 10-30 parts functional additives, and 5-20 parts nutritional fortifiers. This compound formula combines multiple probiotics and optimizes the strain ratio to achieve a synergistic colonization effect. The probiotics are prepared using a freeze-drying process, effectively improving problems caused by flora imbalance. Simultaneously, multiple compound prebiotics provide nutritional support for the proliferation of beneficial bacteria, synergistically enhancing the intestinal regulatory effect. Added functional components such as lactoferrin, chitosan, β-glucan, and poria cocos polysaccharide make it suitable for long-term conditioning consumption by sub-healthy individuals and those with weak digestive systems. The raw sheep milk undergoes vacuum distillation to remove the muttony odor. A process of wet powdering followed by aseptic dry mixing of probiotics is used, resulting in a more stable overall quality of the finished product.

The invention relates to human lactoferrin for use in enhancing bone strength and / or bone growth in a subject.

The present invention relates to a method for producing a lactoferrin-containing product by performing cation exchange with milk having a lactoferrin concentration of at least 4.0 per gram of protein. The invention also relates to a lactoferrin-containing product containing at least 96 wt% of lactoferrin and less than 0.60 wt% of immunoglobulin (IgG + IgA + IgM), based on the total protein.

The invention discloses a lactoferrin gel carrier, a preparation method thereof and application of the lactoferrin gel carrier in an oral delivery system. The preparation method comprises the following steps: mixing a lactoferrin solution with transglutaminase, and reacting under mild conditions to form a gel network. According to the method, gel which is compact in structure and high in stability is formed through enzyme catalytic crosslinking, the mechanical strength and embedding capacity of the gel are remarkably enhanced, and the method is suitable for embedding multiple active ingredients (such as probiotics, polyphenol and vitamins) sensitive to heat, oxygen or shear force. The obtained gel carrier can effectively isolate oxygen, moisture and adverse processing environments, and the stability of embedded components in processing, storage and transportation is remarkably improved. The carrier can be slowly released in a gastrointestinal tract environment, and active ingredients are prevented from being degraded too early in gastric acid, so that the intestinal absorption rate and the bioavailability of the carrier are improved. The materials used in the invention are natural proteins, are safe and non-toxic, and accord with food and health care product application standards.

The invention discloses a carbon dot composite material with anticancer biological activity, and particularly relates to the technical field of carbon dot composite materials, the carbon dot composite material comprises nitrogen ruthenium doped carbon dots and sulfhydrylated lactoferrin, and the molar mass ratio of the nitrogen ruthenium doped carbon dots to the sulfhydrylated lactoferrin is (5-30): 1. In the synthesis process, nitrogen and ruthenium are doped, ruthenium is loaded on an sp2 / sp3 hybrid carbon interface when being doped with carbon dots, Ru / NCDs active carbon dots are formed, nitrogen doping is introduced, the dispersity and stability of ruthenium are enhanced, the agglomeration phenomenon is reduced, the photothermal conversion efficiency of the carbon dot composite material when being applied to a terahertz treatment cabin can be effectively improved, and the application range of the composite material is widened. The nitrogen-ruthenium-doped carbon dots are subjected to surface modification by utilizing sulfhydrylated lactoferrin, the penetrability of the carbon dot composite material to specific cells or tissues can be improved by utilizing the targeting property of the lactoferrin, and the lactoferrin has good biocompatibility, so that the immune response of the carbon dots in a living body can be reduced, and the penetrability of the carbon dots is improved.

1. Name of the design product: Packaging can (Mianyi Lactoferrin Children's Growth Formula Milk Powder). 2. Use of the design product: For packaging milk powder. 3. Design key points of the design product: The combination of shape and pattern. 4. Picture or photo that best shows the design key points: Three-dimensional view.

The invention discloses a pet food containing probiotic-lactoferrin pellets and a preparation method of the pet food. An immune golden triangle formula is adopted, layered wrapping is carried out, active probiotics, prebiotics, protein for enhancing immunity and antioxidant substances are used as formula raw materials, the probiotics are wrapped by the prebiotics and milk fat globule membrane protein, and then the prebiotics and the milk fat globule membrane protein are wrapped by lactoferrin and shiitake mushroom powder. And finally, coating lactoferrin with a coating solution containing antioxidant substances to prepare pellets, and applying the pellets to preparation of pet food. Functional substances such as probiotics and immune protein are delivered to the intestinal tract through multi-layer wrapping, and various functional components capable of improving immunity in the intestinal tract have a synergistic effect, so that the immunity of pets can be remarkably improved.

The invention discloses a nutritional composition for short-time internal inflammation balance after medium-high-strength resistance training and application of the nutritional composition, and belongs to the technical field of food processing. The composition comprises an inflammation control component composed of a plant inflammation control component and a milk source inflammation control component, the inflammation control component is composed of a plant source component and a milk source component and specifically comprises omega-3 unsaturated fatty acid, curcumin, iron unsaturated lactoferrin and a milk fat globule membrane according to a specific weight ratio, the weight ratio of EPA to DHA in the omega-3 unsaturated fatty acid is not lower than 1.5: 1, and the weight ratio of the curcumin to the iron unsaturated lactoferrin to the milk fat globule membrane is not lower than 1.5: 1. The iron saturation of the iron unsaturated lactoferrin is less than or equal to 30%, and the phospholipid accounts for 4-10% of the milk fat ball membrane. Experimental results show that the composition can effectively regulate and control the level of inflammatory factors within 6 hours after training, and accurate regulation and control of inflammatory response within an inflammatory regulation window period within 6 hours after training are achieved. The invention also relates to application of the composition in preparation of functional food and dairy products.