166 results about "Lactoferrin" patented technology

The invention provides modified milk powder capable of promoting bone health as well as a preparation method and application of the modified milk powder. The total protein content of the modified milk powder is 17-35 g / 100 g, the fat content of the modified milk powder is 1-32 g / 100 g, and the calcium content of the modified milk powder is 0.8-2.1 g / 100 g. Moreover, the modified milk powder contains bovine coloctrum and lactoferrin, the mass ratio of the bovine coloctrum to the lactoferrin is (5-100): 1 on the basis of the dry matter content, and the content of the lactoferrin in the modified milk powder is 2-400 mg / 100 g. The bovine colostrum and the lactoferrin are combined for preparing the milk powder, have a synergistic effect on promoting osteoblasts and inhibiting osteoclast proliferation, and are beneficial to bone health.

The invention discloses a method for screening high-yield recombinant human lactoferrin through nucleic acid aptamer-assisted mutagenesis of a trichoderma reesei strain and application, and belongs to the technical field of microorganisms. The nucleic acid aptamer Lac-6a-FAM-BHQ1 capable of changing the fluorescence intensity according to the content of lactoferrin is constructed on the basis of the aptamer Lac-6a, and the nucleic acid aptamer can be used for rapidly selecting a mutant strain for producing high-titer recombinant human lactoferrin. The ARTP mutagenesis technology is combined with a nucleic acid aptamer auxiliary screening system, the mutant strain with the improved recombinant human lactoferrin yield is rapidly obtained from a large number of mutant strains, and the yield reaches 274.57 mg / L and is improved by 24.8% compared with that of an original strain. After the genes 111681 and 62716 are over-expressed in T. reesei, compared with a control strain, the yields of the recombinant human lactoferrin of the recombinant strain are respectively increased by 6.9% and 22.1%.

The present application relates to a nutritional composition, a product comprising the same and use thereof in the preparation of a product for alleviating synaptic plasticity impairment of a body. The nutritional composition comprises: docosahexaenoic acid (DHA) and / or a metabolic precursor thereof, sodium hyaluronate (SH), and lactoferrin (LF). When docosahexaenoic acid (DHA), sodium hyaluronate (SH) and lactoferrin (LF) are used in combination, they can synergistically alleviate synaptic plasticity impairment of a body, in particular, increase nerve growth factor (NGF) content and / or brain-derived neurotrophic factor (BDNF) content in PC12 cells of the body, and / or increase expression of synaptic-related proteins in PC12 cells of the body.

This invention relates to the field of pet care product technology, and discloses a pet oral care spray and its preparation method. The preparation method employs a low-temperature gradient stepwise dissolution process: glucose oxidase is pre-dissolved at low temperature to obtain pre-solution A; bovine colostrum powder and lactoferrin are dissolved at low temperature to obtain protein pre-solution B; honey, folic acid, and vitamin D3 are dissolved at room temperature to obtain excipient pre-solution C; lysozyme is added to protein pre-solution B and stirred to dissolve; then pre-solution A and excipient pre-solution C are combined sequentially, the pH is adjusted, and purified water is added to make up the volume; the mixture is filtered through a microporous membrane for sterilization, and then filled and sealed under inert gas protection. This preparation method effectively protects the biological activity of each active protein and enzyme, achieving a long shelf life without the need for additional chemical preservatives. The resulting spray does not contain irritating ingredients such as alcohol and menthol, and is suitable for improving stomatitis, inhibiting plaque and tartar, and alleviating halitosis in pets of all ages and breeds.

The invention relates to the field of food, in particular to a yeast beta-glucan composition for preventing and relieving upper respiratory tract infection. The composition provided by the invention mainly takes yeast beta-glucan and lactoferrin as main raw materials, and comprises the following components in percentage by weight: 0.1 to 80 percent of yeast beta-glucan and 0.1 to 80 percent of lactoferrin. The composition provided by the invention can also be prepared from other selective synergistic components and auxiliary materials through a series of processing technologies. The composition provided by the invention can ensure the complete structure and activity of the effective components, and has the effects of boosting immunity and preventing and relieving upper respiratory tract infection.

This application discloses a sheep milk probiotic formula milk powder that can regulate intestinal flora and its preparation method. By weight, it contains the following raw material components: 600-700 parts sheep milk base, 5-15 parts compound probiotic powder, 30-60 parts prebiotic composition, 10-30 parts functional additives, and 5-20 parts nutritional fortifiers. This compound formula combines multiple probiotics and optimizes the strain ratio to achieve a synergistic colonization effect. The probiotics are prepared using a freeze-drying process, effectively improving problems caused by flora imbalance. Simultaneously, multiple compound prebiotics provide nutritional support for the proliferation of beneficial bacteria, synergistically enhancing the intestinal regulatory effect. Added functional components such as lactoferrin, chitosan, β-glucan, and poria cocos polysaccharide make it suitable for long-term conditioning consumption by sub-healthy individuals and those with weak digestive systems. The raw sheep milk undergoes vacuum distillation to remove the muttony odor. A process of wet powdering followed by aseptic dry mixing of probiotics is used, resulting in a more stable overall quality of the finished product.

The invention relates to human lactoferrin for use in enhancing bone strength and / or bone growth in a subject.

The invention discloses a lactoferrin gel carrier, a preparation method thereof and application of the lactoferrin gel carrier in an oral delivery system. The preparation method comprises the following steps: mixing a lactoferrin solution with transglutaminase, and reacting under mild conditions to form a gel network. According to the method, gel which is compact in structure and high in stability is formed through enzyme catalytic crosslinking, the mechanical strength and embedding capacity of the gel are remarkably enhanced, and the method is suitable for embedding multiple active ingredients (such as probiotics, polyphenol and vitamins) sensitive to heat, oxygen or shear force. The obtained gel carrier can effectively isolate oxygen, moisture and adverse processing environments, and the stability of embedded components in processing, storage and transportation is remarkably improved. The carrier can be slowly released in a gastrointestinal tract environment, and active ingredients are prevented from being degraded too early in gastric acid, so that the intestinal absorption rate and the bioavailability of the carrier are improved. The materials used in the invention are natural proteins, are safe and non-toxic, and accord with food and health care product application standards.

The invention discloses a nutritional composition for short-time internal inflammation balance after medium-high-strength resistance training and application of the nutritional composition, and belongs to the technical field of food processing. The composition comprises an inflammation control component composed of a plant inflammation control component and a milk source inflammation control component, the inflammation control component is composed of a plant source component and a milk source component and specifically comprises omega-3 unsaturated fatty acid, curcumin, iron unsaturated lactoferrin and a milk fat globule membrane according to a specific weight ratio, the weight ratio of EPA to DHA in the omega-3 unsaturated fatty acid is not lower than 1.5: 1, and the weight ratio of the curcumin to the iron unsaturated lactoferrin to the milk fat globule membrane is not lower than 1.5: 1. The iron saturation of the iron unsaturated lactoferrin is less than or equal to 30%, and the phospholipid accounts for 4-10% of the milk fat ball membrane. Experimental results show that the composition can effectively regulate and control the level of inflammatory factors within 6 hours after training, and accurate regulation and control of inflammatory response within an inflammatory regulation window period within 6 hours after training are achieved. The invention also relates to application of the composition in preparation of functional food and dairy products.

The application belongs to the field of food functional factor processing, and particularly relates to a water-in-oil-in-water composite emulsion embedding lactoferrin, a preparation method and application. The W1 / O / W2 double emulsion system is constructed by a two-step method, and the leakage and inactivation risks of lactoferrin and VD3 in the preparation, storage and processing processes are effectively reduced through the double barrier effect of the oil phase film and the outer water phase film. Under the optimized sodium caseinate and gellan gum compounding conditions, the encapsulation rate of lactoferrin can be up to 82.52%, which proves that the structure has obvious advantages in improving the loading efficiency of active substances, and can significantly improve the encapsulation rate and structural stability of lactoferrin. The water-in-oil-in-water composite emulsion system prepared by the application can significantly improve the retention rate of lactoferrin in the digestion process, enhance the tolerance of lactoferrin to the gastrointestinal environment, and then enhance the arrival rate of lactoferrin in the intestinal tract, thereby improving the overall bioavailability.

The application discloses a multi-effect repairing and anti-allergy toothpaste composition and a preparation method thereof. The toothpaste comprises the following raw material components in percentage by mass: hydroxyapatite 1.0-10.0%, zeolite 0.02-0.8%, vitamin E 0.02-0.5%, lactoferrin 0.0005-0.02%, and the rest is base. When harmful bacteria form a biofilm in the oral cavity, the zeolite can destroy the biofilm formed by the harmful bacteria, so that the vitamin E and the lactoferrin enter the biofilm. The lactoferrin adsorbs iron ions near the harmful bacteria, so that the harmful bacteria cannot form a biofilm again. After rinsing, a large amount of water can take away the harmful bacteria, and finally the hydroxyapatite repairs. The effective synergistic closed loop of the hydroxyapatite, the zeolite, the vitamin E and the lactoferrin reconstructs a healthy oral micro-ecology from the source.

The present application relates to the field of biosynthesis and microbial fermentation engineering technology, in particular to a kind of secretion expression human lactoferrin Yarrowia lipolytica and its construction method and application.The present application provides a kind of secretion expression human lactoferrin recombinant Yarrowia lipolytica, the secretion expression of human lactoferrin in the recombinant Yarrowia lipolytica is guided by secretion signal peptide;The expression of chaperone HAC1, Cne and Pdi1 is simultaneously enhanced.The present application realizes the secretion expression of human lactoferrin in Yarrowia lipolytica, effectively promotes the folding and secretion expression of human lactoferrin by the cooperation of chaperone, significantly improves the yield of human lactoferrin, and has important application value in the industrial scale production of human lactoferrin.

Provided are use of a bioactive protein in the preparation of a drug for treating herpes virus infection and a pharmaceutical composition for treating herpes virus infection. The pharmaceutical composition mainly comprises a bioactive protein, a matrix, a humectant, a pH regulator, and a preservative. The present invention has the following technical effects: 1) By means of a large amount of research and experimental verification, the inventors have discovered for the first time that anhydridized lactoferrin, a modified bioactive protein, has an inhibitory effect on herpes zoster virus (VZV). 2) In the pharmaceutical composition, the type and amount of excipient and the proportion of the main ingredient are reasonable. The pharmaceutical composition is administered in ointment form, in spray form, or in other dosage forms suitable for external use, has a significant therapeutic effect, has small local toxic side effects, has very low acute dermal toxicity, causes no irritation or allergy, does not interfere with normal skin functions, and is safe and effective.

The invention provides an oral care composition for inhibiting helicobacter pylori and a preparation method of the oral care composition. The oral care composition comprises glycerin, xylitol, erythritol, thymol clathrate, garlic extract clathrate, propolis extract, lactoferrin, disodium ethylene diamine tetraacetate, menthol, potassium sorbate and purified water. The thymol clathrate compound is obtained by carrying out clathration on thymol through beta-cyclodextrin. The garlic extract clathrate compound is obtained by clathrating a garlic extract with beta-cyclodextrin. According to the oral care composition provided by the invention, the helicobacter pylori in the oral cavity can be effectively inhibited through reasonable proportioning of various components, so that the risks of ozostomia and stomach reinfection are reduced.

This invention discloses a camel milk formula powder for assisting in the regulation of blood lipids and its preparation process, belonging to the field of functional dairy product deep processing technology. This formula powder constructs a five-in-one synergistic lipid-regulating network of "exogenous absorption inhibition, endogenous synthesis regulation, oxidative stress protection, intestinal microecological regulation, and vascular endothelial protection." Its core components include a specific ratio of compounded phytosterol esters, microencapsulated complex oils, and medicinal and edible extracts. The preparation process employs stepwise low-temperature microencapsulation and cold spray agglomeration granulation technology to achieve full-process low-temperature protection of active ingredients, with lactoferrin retention ≥90% and ω-3 fatty acid bioavailability increased by more than 25%. This invention's product has comprehensive lipid-regulating effects, 3 times improved oxidative stability, and produces no oil residue or off-odor when mixed, making it suitable for long-term consumption by people with borderline elevated blood lipids. It can also achieve continuous industrial production with a batch mixing uniformity RSD ≤2%.

The application provides a high-stability DHA emulsion and a preparation method thereof. The high-stability DHA emulsion comprises the following raw materials in parts by mass: 20-40 parts of DHA algal oil; 1-3 parts of phospholipid; 2-5 parts of emulsifier; 0.05-0.3 parts of fat-soluble antioxidant; 0.05-0.3 parts of water-soluble antioxidant; 2-3 parts of lactoferrin-reducing oligosaccharide conjugate; 0.5-2 parts of polysaccharide; and 55-75 parts of water. The high-stability DHA emulsion can be used as a nutritional fortifier for milk and dairy products, beverages and formula food.

A nutritional composition for an Alzheimer's disease population or a high-risk population, prepared from the following raw materials in parts by weight: 170-300 parts of proteinaceous substances, 85-250 parts of fatty substances, 450-770 parts of carbohydrates, 10-155 parts of dietary fiber substances, 1.2-3.5 parts of trace minerals, 47-180 parts of macro minerals, 2.0-8.3 parts of vitamins, 0.1-1.0 parts of taurine, 0.05-0.5 parts of L-carnitine, and 0.9-1.0 parts of lactoferrin. The composition is rich in high-quality animal and plant proteins, fatty acids, various vitamins, minerals, and water-soluble dietary fibers, has remarkable effects in slowing down the occurrence and development of Alzheimer's disease, and is suitable for an Alzheimer's disease population or a high-risk population to eat.

This invention discloses a kitten food without palatability enhancers and its preparation method, relating to the field of pet food technology. It comprises the following components: polypeptide chicken meal, soy protein peptides, gluten hydrolysate, lactoferrin, astragalus extract, yam powder, rosemary oil, resveratrol, tributyrate, and rice. By adding substances containing small peptides and plant extracts to the kitten food, this invention provides the body with a large amount of high-quality animal protein without adding any chemically synthesized palatability enhancers, preservatives, or other chemical additives. This effectively promotes the body's decomposition and metabolism of nutrients, promotes the growth of kittens, and also enhances the body's antioxidant capacity and reduces the occurrence of diarrhea.

This invention belongs to the field of cosmetic technology, specifically relating to an anti-aging composition containing lactoferrin and its application. The anti-aging composition provided by this invention, through scientific compounding, achieves synergistic effects in anti-aging among four components: medicinal Fomitopsis thunbergii extract, butterfly pea flower extract, Artemisia annua extract, and lactoferrin. This results in excellent elastase inhibition, preventing the degradation of elastin in the dermis and effectively strengthening the elastin scaffold, thereby enhancing the anti-aging effect of the composition. When applied to a prepared emulsion, this anti-aging composition can improve skin firmness (R0 change rate), skin elasticity (R2 change rate), and facial wrinkles (wrinkle change rate), exhibiting anti-aging effects of improving wrinkle reduction and firmness, and promoting skin elasticity. Furthermore, it does not cause skin irritation, possessing safe and gentle characteristics, a wide range of applications, and high consumer acceptance.

The invention provides a biotinylated Angiopep-2 modified targeting nano material as well as a preparation method and application of the biotinylated Angiopep-2 modified targeting nano material. According to the preparation method of the biotinylated Angiopep-2 modified targeting nano-material, Angiopep-2 is directionally anchored through a high-affinity biotin-streptavidin system, ligand conformation inactivation caused by chemical coupling is avoided, targeting activity is maintained, the used Angiopep-2 can help nanoparticles to be specifically recognized and combined and mediate the nanoparticles to cross a blood brain barrier to enter the brain, and the targeting nano-material can be used for preparing the targeted nano-material. The drug concentration in brain tissues is increased, so that the cure rate of brain diseases is improved, the brain targeting efficiency is far higher than that of transferrin and lactoferrin, and the application value is great; by entrapping curcumin and quercetin, a hydrophilic shell is provided, so that the water solubility of curcumin and quercetin is improved, and the bioavailability of curcumin and quercetin is improved.

The present invention relates to a synergistic bioactive composition for women's health, and more particularly to compositions comprising Lactobacilli and Lactoferrin for supporting vaginal health. The invention specifically provides a composition comprising Lactobacillus crispatus, Lactobacillus jensenii, Lactobacillus fermentum, and Lactoferrin, formulated with one or more pharmaceutically acceptable excipients. The invention further pertains to compositions and kits intended for promoting a balanced vaginal microbiota, maintaining healthy vaginal conditions, and supporting overall vaginal ecosystem stability.

The invention relates to the technical field of food processing, in particular to frozen camel milk with milk skin and a preparation method thereof.The preparation method comprises the following steps that firstly, after camels produce milk, the temperature of the raw material camel milk is reduced to 0-2 DEG C from 37 DEG C within 10 minutes, the raw material camel milk is stored in 304 stainless steel, and nitrogen is introduced into a tank for protection during storage; step 2, after the raw milk reaches a factory, the total number of bacterial colonies is less than or equal to 20000 CFU / g, the protein content is more than or equal to 3.8 g / 100g, and the fat content is more than or equal to 4.5 g / 100g; through a series of measures such as rapid cooling, low-temperature storage, nitrogen introduction for protection, production and processing time shortening, low-temperature centrifugal separation, appropriate-temperature mixing and stirring, rapid cooling and the like, microbial reproduction is effectively inhibited, the oxidation speed of fat and protein in camel milk is slowed down, and decomposition and loss of nutritional ingredients are reduced; active nutrient substances in the camel milk are reserved to the maximum extent, and meanwhile, the content of active lactoferrin in the stock solution is increased.

The invention discloses a preparation technology of a lactoferrin and osteopontin compound capable of improving bone health, and belongs to the technical field of food processing. In the compound, the mass ratio of lactoferrin to osteopontin is (3-5): 1, and the pH is 4-5.5. In a zebra fish model, after intervention of the lactoferrin, the osteopontin, the compound or the mixture, compared with a mixture group, the compound group of the lactoferrin and the osteopontin can obviously improve the fluorescence intensity of zebra fish spines, the relative bone mineral density is improved by 31.12%, and compared with a blank group, the relative bone mineral density is improved by 2.39 times. Therefore, the lactoferrin and osteopontin compound with the function of improving bone health can be prepared, and the lactoferrin and osteopontin compound has good application potential.

The utility model discloses a kind of lactoferrin molecule balance refining reaction devices, the utility model relates to lactoferrin refining processing technical field, including cylinder, the top of cylinder is provided with cylinder cover, the bottom of cylinder cover is provided with centrifugal component, the inner wall of cylinder is provided with scraping component;Centrifugal component includes feed rotary pipe, the bottom outer wall of feed rotary pipe is connected with connecting pipe, the bottom of connecting pipe is fixedly connected with centrifugal cylinder, scraping component includes rotary ring, the outer wall of rotary ring is fixedly installed with connecting plate, the outer wall one end of connecting plate is fixedly installed with spiral scraping strip, spiral scraping strip is attached with the inner wall of cylinder, the related structure of centrifugal component setting makes centrifugal cylinder easy to disassemble and install.This facilitates after refining process ends, centrifugal cylinder is cleaned and maintained, scraping component can drive spiral scraping strip rotation, liquid adhered in the inner wall of cylinder is scraped, promotes liquid discharge, ensure that the liquid in cylinder can be normally discharged.

The invention discloses a method for detecting lactoferrin in milk based on a competition method test strip, which comprises the following steps: coupling heparin molecules with bovine serum albumin to obtain a heparin molecule-bovine serum albumin artificial antigen; modifying gold nanoparticles by using an artificial antigen to form a gold label I, and constructing a competition method test strip by matching with a gold label II modified by mouse IgG (Immunoglobulin G); a milk sample is subjected to centrifugal fat removal, pH adjustment and casein precipitation pretreatment and then dropwise added to the test strip, and the LF concentration is calculated through a T line gray value and a working curve. Detection can be completed within about 10-15 min, specificity is high (interference of impure protein is avoided), on-site rapid quantitative / semi-quantitative detection of LF in milk can be achieved, the antiviral activity of LF can be indirectly reflected through the binding strength of heparin and LF, the method is suitable for production quality inspection, dealer sampling inspection and consumer self-inspection, and the detection sensitivity reaches 1 microgram / mL.

The invention relates to the technical field of milk powder, and particularly discloses infant LMNP combined formula milk powder for caesarean delivery and a preparation method of the infant LMNP combined formula milk powder for caesarean delivery, 100g of formula milk powder comprises an intestinal micro-ecological regulation composition, an immune nutrition synergistic composition, a core nutrition component and a vitamin and mineral composition. The composition has the advantages that the composition is reasonable in formula, beneficial bacteria colonization and proliferation are synergistically promoted, healthy intestinal flora is quickly established, common intestinal flora imbalance of the cesarean delivery infants is effectively improved, the risks of diarrhea, constipation and the like are reduced, and an immune nutrition synergistic system is formed through scientific compatibility of lactoferrin, milk fat globule membranes and compound nucleotide. The composition can enhance immune cell activity, promote immune system development, strengthen mucous membrane defense, make up immune shortages of caesarean section babies in a targeted manner, and reduce allergy and infection risks.

The invention provides a rare ginsenoside nano-liposome, a preparation method thereof and application of the rare ginsenoside nano-liposome in improving sleep and relieving physical fatigue. The preparation method comprises the following steps: (1) preparing a lactoferrin solution; (2) preparing a rare ginsenoside solution; (3) preparing a thin film; (4) adding a rare ginsenoside solution into the film, and carrying out ultrasonic treatment to obtain a rare ginsenoside crude liposome suspension; (5) slowly dropwise adding the lactoferrin solution into the rare ginsenoside crude liposome suspension, and stirring for cross-linking reaction to obtain a rare ginsenoside crude liposome solution; and (6) carrying out high-pressure micro-jet nano-homogenization on the rare ginsenoside crude liposome solution to obtain the rare ginsenoside nano-liposome. The invention solves the technical problems of low safety, poor stability, low targeting property and low bioavailability of the traditional nano-liposome, and belongs to the technical field of functional foods.

The invention provides a no-wash antibacterial liquid for private parts and a preparation method of the no-wash antibacterial liquid. The mucous membrane repairing agent is prepared from the following raw materials in percentage by weight: 0.08 to 0.16 percent of polyhexamethylene biguanide hydrochloride, 0.9 to 1.9 percent of chitosan-lactoferrin-lactate grafted copolymer, 1.3 to 2.3 percent of sodium alginate coated nano zinc-plant prebiotic microcapsules, 0.1 to 0.3 percent of mucous membrane repairing agent, 1.2 to 2.2 percent of micro-ecological regulator, 2.8 to 5.2 percent of moisturizing soothing agent, 0.35 to 0.75 percent of thickening stabilizer, 1.4 to 2.8 percent of preservative, 0.6 to 1.4 percent of pH regulator, 1.2 to 2.6 percent of emulsifier and the balance of deionized water. According to the invention, all the components have a synergistic effect, and the safe and mild no-wash antibacterial liquid for the private parts, which has the functions of real-time bacteriostasis, lasting protection, mucous membrane repair and micro-ecological balance, is provided.

The invention discloses a milk-derived active peptide DDLCDYWIR with strong antioxidation and immunoregulation as well as a preparation method and application thereof, and relates to the field of active peptides. According to the invention, lactoferrin is used as a raw material, and after protease enzymolysis and lactobacillus paracasei fermentation treatment are carried out, a novel milk-derived active peptide is screened from the product. Experiments prove that the active peptide shows excellent antioxidant activity and immune regulation effect, not only can enhance the immune function of the body, but also can effectively resist oxidative stress and play a role in delaying senescence. Meanwhile, the active peptide is derived from a natural milk source, is not added with any chemical substance, has good biocompatibility, and can be widely applied to preparation of antioxidant and immunomodulatory products or products with the antioxidant and immunomodulatory functions.