414 results about "Lactoferrin" patented technology

The invention relates to the technical field of nutritional supplements. A traditional fat reducing method mainly comprises dieting, high-intensity exercise, medicine weight losing and the like. However, dieting may lead to malnutrition; high-intensity sports are difficult to insist, and sports injury is easily caused; in order to solve the technical problems, the invention discloses a fat-reducing nutritional composition capable of improving metabolism, and the fat-reducing nutritional composition can be used for solving the technical problems of severe side effects such as tachycardia, insomnia and the like caused by medicine weight loss. The composition comprises the following components in parts by weight: 13-21 parts of a bitter gourd extract, 15-31 parts of a garcinia cambogia extract, 13-17 parts of oat fibers, 17-23 parts of whey protein powder, 7-9 parts of linseed oil, 6-14 parts of a green tea extract, 9-15 parts of a calcium supplement and 15-19 parts of a purple perilla seed extract, and can provide energy for a user, supplement immune globulin, lactoferrin and a calcium source, improve the immunity of the user and improve the immunity of the user. The body metabolism level and immunocompetence are improved, absorption of fat in digestive tracts can be effectively inhibited, storage of fat in the body is reduced, fat consumption is promoted, and the fat reducing effect is achieved.

The invention belongs to the field of nutrient substance research, and particularly relates to application of a nutrient composition to regulation of brain immunity. The invention provides application of a nutritional composition in preparation of food for regulating brain immunity. Wherein the nutritional composition comprises the following necessary components: lactoferrin and fucosyllactose, and in the nutritional composition, the mass ratio of the lactoferrin to the fucosyllactose is 1: (0.1-30). Research finds that after lactoferrin and fucosyllactose are combined according to a certain proportion, the lactoferrin and fucosyllactose have a synergistic effect of regulating brain immunity. Experimental data show that after the combination of lactoferrin and fucosyllactose is used for treating the BV2 cells, the trend that the BV2 cells are differentiated into M2 phenotype microglial cells is higher than the trend that the BV2 cells are differentiated into M1 phenotype microglial cells, and the amount of secreted inflammatory factors is obviously reduced.

The present invention belongs to the field of biomedicine, and specifically relates to an antimicrobial peptide derived from lactoferrin and its application. The antimicrobial peptide derived from lactoferrin, whose amino acid sequence is shown in any one of SEQ ID NOs: 1 to 98, has a broad-spectrum antimicrobial effect, especially significant antibacterial effect against Pseudomonas putida, Rhodococcus erythropolis, Bacillus subtilis, Malassezia furfur, Cryptococcus neoformans, and Candida albicans, and has great application value.

The invention discloses an application of knockout of PEP4 and BAR1 genes in reduction of degradation of Kluyveromyces marxianus on lactoferrin. The invention provides application of inhibiting expression of PEP4 protein and BAR1 protein in any one of the following aspects: (A1) reducing degradation of Kluyveromyces marxianus on lactoferrin; and (A2) reducing the degradation of the fermentation supernatant of the kluyveromyces marxianus on lactoferrin. Experiments prove that after the encoding gene of the PEP4 protein and the encoding gene of the BAR1 protein are knocked out in yeast cells, the lactoferrin degradation amount can be reduced, the complete protein amount is remarkably increased, and the method has important significance on increasing the secretory expression yield of the lactoferrin in kluyveromyces marxianus.

The invention provides a milk powder composition containing breast milk oligosaccharide and easy to digest and absorb. The milk powder composition comprises the breast milk oligosaccharide, OPN, galactooligosaccharide, lactoferrin, alpha-whey protein, multivitamins, lactose, vegetable oil and compound minerals, wherein the weight ratio of the lactoferrin to the OPN to the breast milk oligosaccharide to the galactooligosaccharide is 1: (2-4): (2-50): (4-100). The composition can effectively recover and improve the intestinal barrier function caused by inflammatory factors, can be well absorbed by infants with poor digestion and absorption functions, reduces diarrhea and promotes skeletal development.

The invention belongs to the technical field of medical nano-materials, and particularly relates to a lactoferrin nano-material for sonodynamic regulation and control of blood-brain barrier permeability as well as a preparation method and application of the lactoferrin nano-material. The nanomaterial is nanoparticles LCe6 formed by self-assembly of lactoferrin and chlorin e6, can target brain microvascular endothelial cells, and can temporarily, reversibly and targeting open BBB by using the sonodynamic effect of the LCe6 in combination with transcranial ultrasonic stimulation, so that the accumulation of the nanoparticles LCe6 at a focus part is improved; in addition, the nanoparticle LCe6 also has a targeting property on brain tumor cells, the tumor cells can be accurately killed by utilizing the sonodynamic effect of the nanoparticle under secondary transcranial ultrasound, and the effect of sonodynamic treatment on brain glioma is exerted to the maximum extent; the nanoparticles LCe6 have excellent biocompatibility and low toxicity; according to the invention, the intracerebral delivery efficiency of the drug can be obviously improved, and a novel method for targeting open brain disease BBB is provided.

The invention discloses a method for preparing low-bitterness micromolecular bovine colostrum protein peptide powder by adopting a multi-stage membrane separation and enzyme membrane coupling combined technology, and belongs to the technical field of food processing. The preparation method comprises the following steps: firstly, dividing a colostrum component into components to be hydrolyzed by adopting a multi-stage membrane separation technology, namely casein intercepted by ceramic membrane microfiltration and lactoglobulin and lactalbumin filtered by an ultrafiltration membrane, and non-hydrolytic components, namely immune globulin and lactoferrin; the preparation method comprises the following steps: firstly, separating bovine coloctrum, then deeply hydrolyzing the separated lactoglobulin, milky white and casein flocculate with the molecular weight of less than 50kDa by adopting an enzyme membrane coupling technology, mixing with unhydrolyzed immunoglobulin and lactoferrin, and freeze-drying, so that the peptide content in the treated bovine coloctrum is more than 40%, the peptide content of 200-1000Da is more than 30%, the peptide content of 200-1000Da is more than 30%, and the peptide content of 200-1000Da is more than 30%. The preparation method is simple, the preparation dispersion time is within 10 s, no obvious bitter taste exists, the retention rate of immune globulin in bovine coloctrum reaches 90% or above, and the preparation method is suitable for large-scale industrial popularization.

The invention relates to the technical field of food processing, in particular to antioxidant nano collagen peptide powder and a preparation method thereof.The antioxidant nano collagen peptide powder is prepared by forming antioxidant micelles wrapping collagen peptide through collagen peptide liquid and a complex antioxidant, loading the antioxidant micelles on a porous carrier and then conducting cross-linking through transglutaminase; tea polyphenol and whey protein are covalently combined to prepare a composite antioxidant, rapid free radical neutralization, lactoferrin inhibition of an oxidation source and synergistic effect of collagen peptide self-antioxidant groups are provided, multistage barrier design is combined, oxygen diffusion is isolated through a micelle hydrophobic core, and the contact area of micelles and the outside is reduced through physical adsorption of a porous carrier, so that the anti-oxidation effect of the collagen peptide is improved. Finally, the micelles are fixed in the carrier by utilizing an enzyme cross-linked network, so that the antioxidant nano collagen peptide powder has efficient and long-acting antioxidant capacity.

The invention provides modified milk powder capable of promoting bone health as well as a preparation method and application of the modified milk powder. The total protein content of the modified milk powder is 17-35 g / 100 g, the fat content of the modified milk powder is 1-32 g / 100 g, and the calcium content of the modified milk powder is 0.8-2.1 g / 100 g. Moreover, the modified milk powder contains bovine coloctrum and lactoferrin, the mass ratio of the bovine coloctrum to the lactoferrin is (5-100): 1 on the basis of the dry matter content, and the content of the lactoferrin in the modified milk powder is 2-400 mg / 100 g. The bovine colostrum and the lactoferrin are combined for preparing the milk powder, have a synergistic effect on promoting osteoblasts and inhibiting osteoclast proliferation, and are beneficial to bone health.

The invention discloses a method for screening high-yield recombinant human lactoferrin through nucleic acid aptamer-assisted mutagenesis of a trichoderma reesei strain and application, and belongs to the technical field of microorganisms. The nucleic acid aptamer Lac-6a-FAM-BHQ1 capable of changing the fluorescence intensity according to the content of lactoferrin is constructed on the basis of the aptamer Lac-6a, and the nucleic acid aptamer can be used for rapidly selecting a mutant strain for producing high-titer recombinant human lactoferrin. The ARTP mutagenesis technology is combined with a nucleic acid aptamer auxiliary screening system, the mutant strain with the improved recombinant human lactoferrin yield is rapidly obtained from a large number of mutant strains, and the yield reaches 274.57 mg / L and is improved by 24.8% compared with that of an original strain. After the genes 111681 and 62716 are over-expressed in T. reesei, compared with a control strain, the yields of the recombinant human lactoferrin of the recombinant strain are respectively increased by 6.9% and 22.1%.

The invention belongs to the technical field of biological medicines, and particularly relates to application of bifidobacterium breve and lactoferrin in preparation of products for regulating intestinal flora or resisting bacterial infection. The invention provides application of Bifidobacterium breve HH079 with the preservation number of GDMCC No: 64216 and lactoferrin in preparation of a product for regulating intestinal flora or a product for resisting bacterial infection, and the Bifidobacterium breve HH079 and the lactoferrin are combined and synergistically used for resisting the bacterial infection. The composition can reverse weight loss, colon shortening, bacterial load increase and colon epithelium injury caused by bacterial infection, can significantly inhibit the level of proinflammatory cytokines, promote the expression of anti-inflammatory cytokines, can significantly increase the concentration of acetic acid and propionic acid in excrement of infected mice, can generate positive regulation on intestinal flora, and can inhibit bacterial infection. Good application prospects are shown in regulation of intestinal flora and bacterial infection resistance.

The invention discloses a nutritional composition beneficial to pet health and pet food containing the nutritional composition. The nutritional composition comprises beta-glucan, plasma protein and lactoferrin. Wherein the mass ratio of the beta-glucan to the plasma protein to the lactoferrin is (0.055 to 0.275) to (1.56 to 4.68) to (0.00095 to 0.0285). The nutritional composition beneficial to pet health provided by the invention is used for improving intestinal health of pets and improving digestive ability; the immunity of the pets can be improved, and the hair health of the pets is improved.

The present invention discloses a lactoferrin composition and its application for improving immunity by regulating macrophage immune activity, and belongs to the technical field of functional compositions. The present invention obtains a lactoferrin composition with regulating macrophage immune activity by compounding lactoferrin, α-lactalbumin, N-acetylneuraminic acid and yeast β-glucan in a specific ratio, the raw material of the composition is easy to obtain, and four components work together to significantly enhance its phagocytic activity without changing the activity of macrophages, regulate the polarization state of macrophages in inflammatory response, inhibit macrophages from polarizing to pro-inflammatory M1 type, and promote macrophages to polarize to anti-inflammatory M2 type; At the same time, the composition can also enhance the antigen presentation ability of macrophages and the activation of immune T cells, and improve body immunity. Therefore, the composition provided by the present invention can be used to prepare health products, food or medicines, etc. that improve immunity.

The invention relates to the technical field of fermentation preparation devices, and particularly discloses a lactoferrin yoghourt fermentation preparation device and method.The lactoferrin yoghourt fermentation preparation device comprises a rack and a fermentation tank fixedly installed in the rack, a transfer tank is fixedly installed on the outer side of the fermentation tank, a material pouring and defoaming mechanism is arranged in the fermentation tank, and a stirring mechanism is arranged at the bottom of the material pouring and defoaming mechanism; a follow-up material pumping and pressing mechanism is arranged between the transfer tank and the fermentation tank, a fluid channel of a material pressing cone is composed of three sections, each section is responsible for treating bubbles of different sizes, and a bottom strong shearing section rapidly breaks small bubbles through high shearing force and shears large bubbles into multiple medium or small bubbles; the middle pressure-adaptive section helps bubbles with different sizes to gradually float through moderate pressure difference and shearing force; and the top pressure reduction section ensures that all residual bubbles can smoothly emerge from the surface and are discharged through a low-pressure environment.

The invention relates to the technical field of pet food, in particular to flavoring agent powder for pet dogs and a preparation method of the flavoring agent powder. Comprising the following raw materials: 40 parts of beef liver, 10 parts of hairtail, 7-40 parts of ricefield eel, 1 part of protease, 0.5-1 part of lipase, 0.5-1 part of flavor enzyme, 5-10 parts of saccharides, 10 parts of fish oil, 5-10 parts of maltodextrin, 0.5-1 part of sodium hydroxide, 5 parts of phosphoric acid, 3 parts of amino acid, 2 parts of thiamine, 1 part of yeast extract and 0.5-1 part of lactoferrin. The dog feed can increase the appetite of dogs from the sense of smell, maintain the health balance of intestinal flora of dogs and improve the micro-ecological environment of intestinal tracts.

The invention discloses sheep colostrum immune globulin powder and a preparation process thereof, and relates to the technical field of protein powder. The sheep colostrum immune globulin powder is prepared from the following components in parts by weight: 200 to 250 parts of a sheep colostrum raw material, 350 to 400 parts of whole milk powder, 330 to 370 parts of skimmed milk powder, 30 to 50 parts of prebiotics, 8 to 12 parts of acerola cherry powder, 0.8 to 1.2 parts of lactoferrin, 0.4 to 0.6 part of N-acetylneuraminic acid and 0.3 to 0.5 part of probiotics. Diversified nutritional requirements of a human body are fully considered, various nutritional ingredients are matched with one another and have a synergistic effect, the nutritional ingredients can be better absorbed and utilized by the human body, comprehensive nutritional support is provided for consumers, and the requirements of daily nutritional supplement, physique enhancement, immunity improvement and the like are met.

The utility model relates to the technical field of cow milk protein separation and purification, and provides a cow milk protein component synchronous separation system. Comprising a milk bin, a fat separator, a first cation exchange device, a first membrane filtration module, a casein solution treatment branch, a second membrane filtration module, a second cation exchange device, a first anion exchange device, a second anion exchange device, a purification and sterilization treatment branch and a product production line. Membrane filtration and a chromatographic technology are combined, and different active proteins are separated by using the molecular weight, the charged property, the hydrophobic interaction and the like of the proteins. Main active protein in milk can be separated, beta-casein, alpha-lactalbumin, immune globulin and lactoferrin can be sequentially separated and purified from raw milk, drying or sterile filling is carried out, different products are prepared while natural nutritional ingredients are reserved, and in addition, the product quality is improved. The separated milk can still be processed into sterilized milk or milk beverages.

The invention discloses double-milk-source fermented milk of double-peak camel milk and brown milk and a preparation method of the double-milk-source fermented milk, and the preparation method comprises the following steps: mixing raw materials: mixing the double-peak camel milk and the brown milk according to a mass ratio to obtain double-milk-source mixed milk; pretreatment: sequentially carrying out homogenization treatment and heat treatment on the double-milk-source mixed milk; strain inoculation: inoculating the pretreated double-milk-source mixed milk with a fermentation strain, wherein the fermentation strain comprises a core strain and an auxiliary strain; the core strains are lactobacillus and streptococcus thermophilus which have tolerance to antibacterial components of the camel milk, and the core strains contain strains capable of producing exopolysaccharides. Through scientific matching of lactobacillus, streptococcus thermophilus and specific auxiliary strains which have tolerance to antibacterial components of the camel milk, the inhibition effect of natural antibacterial components such as lysozyme and lactoferrin in the camel milk on conventional strains is successfully overcome.

The invention discloses temperature-sensitive antibacterial hydrogel based on ultra-small-particle-size copper nanoparticles and a preparation method of the temperature-sensitive antibacterial hydrogel, and relates to the technical field of biomedical materials. The preparation method of the temperature-sensitive antibacterial hydrogel comprises the step of uniformly mixing the gel matrix, the stabilizer, the pH buffer solution and the ultra-small-particle-size copper nanoparticles to prepare the temperature-sensitive antibacterial hydrogel. The preparation method of the copper nanoparticles with the ultra-small particle size comprises the following steps that lactoferrin is dissolved in water, copper sulfate pentahydrate is added, an alkali solution is added to adjust the pH of the solution to be 8-14, then a reducing agent is added for a reaction, dialysis and drying treatment are conducted, and the copper nanoparticles with the ultra-small particle size are obtained. The hydrogel has good injectability, can release the copper nanoparticles under specific conditions and rapidly sterilize, in addition, the hydrogel is convenient to synthesize and simple to operate, has good biocompatibility, shows relatively low cytotoxicity to cells, and has relatively great application potential in the process of treating skin wound infection.

The invention discloses a resveratrol delivery system based on lactoferrin-polyglutamic acid composite nanogel and a preparation method thereof.The preparation method comprises the steps that a lactoferrin aqueous solution and a gamma-sodium polyglutamate aqueous solution are mixed to form a composite system, resveratrol is embedded with the composite system, and the resveratrol delivery system is obtained. And finally, adding calcium chloride into the resveratrol-loaded lactoferrin / polyglutamic acid mixed system, so that the calcium chloride and calcium ions are subjected to ionic crosslinking to form stable nanogel, and freeze-drying the nanogel to obtain the high-resveratrol nanogel powder. According to the invention, lactoferrin and polyglutamic acid are selected to be compounded to construct a resveratrol-loaded lactoferrin / polyglutamic acid nanogel system, a composite structure is formed by utilizing electrostatic interaction between protein and peptide polyacid, and then the composite structure is crosslinked with Ca < 2 + > to form a stable core-shell type three-dimensional nanogel structure. The water solubility, the drug loading capacity and the biological accessibility of the resveratrol are obviously improved.

The invention relates to the technical field of high-activity immune globulin liquid extraction, in particular to a preparation method for extracting high-activity immune globulin liquid from bovine colostrum. The invention discloses a preparation method for extracting high-activity immune globulin liquid from bovine coloctrum, which comprises the following steps: performing primary separation, secondary separation, filtration, ultrafiltration concentration and sterilization on degreased bovine coloctrum to obtain the high-activity immune globulin liquid, the primary separation comprises the following steps: adjusting the pH value of the defatted bovine colostrum to 4.6-4.7, and sieving to remove casein, so as to obtain whey; and the secondary separation comprises the following steps: adjusting the pH value of the whey to 3.8-4.0, uniformly mixing, and sieving to remove denatured protein to obtain an immunoglobulin liquid. The high-activity immune globulin liquid containing IgG, IgA, IgM, lactoferrin, lactoperoxidase, IGF-beta, lysozyme, lactose and protein, which is prepared by adopting the method disclosed by the invention, can be used for effectively improving the immunity of a human body.

The invention discloses pichia pastoris for efficiently synthesizing lactoferrin as well as a construction method and application of the pichia pastoris, and belongs to the technical field of biosynthesis. The pichia pastoris for efficiently synthesizing the lactoferrin expresses at least one of KAR2, PDI1 and ERO1 in molecular chaperones in a host in an enhanced manner and carries an optimized secretory signal peptide, and the secretory signal peptide is obtained by replacing a leading peptide of alpha-factor with any signal peptide in LacZ, PelB, FLO and alpha-factor. The yield of LF can be effectively increased by enhancing expression of at least one of KAR2, PDI1 and ERO1 molecular chaperones in molecular chaperones in a host; when the lactoferrin expression cassette is integrated into the recombinant pichia pastoris X-33 genome and the copy number is increased to 2-6, the LF yield can be further increased, and when the copy number is 4, the LF yield reaches a peak value.

The invention provides freeze-dried pet food capable of improving intestinal functions of pets and a preparation method of the freeze-dried pet food. Firstly, lecithin and beta-sitosterol are used for constructing lipidosome embedded compound bacteria, and then lactoferrin is combined with embedded compound enzyme to form microspheres; chitosan oligosaccharide is used for connecting compound bacteria liposome and compound enzyme microspheres, the chitosan oligosaccharide is loaded in an alginate gel network to prepare a compound microcapsule, finally raw materials such as yoghourt, camel milk, milk mineral salt and galactooligosaccharide are mixed, and the freeze-dried pet food is prepared through vacuum freeze drying. The sodium alginate composite microcapsule prepared by the invention can greatly improve the stability of probiotics and enzymes, ensure the activity of the probiotics and the enzymes, and improve the palatability of pets to daily ration, increase the food consumption of the pets, promote the digestion and absorption of nutrient substances by the intestinal tracts of the pets and improve the immunity of the pets by virtue of targeted release of the intestinal tracts and synergistic effect of the prebiotics. The intestinal function of the pet is improved, and an efficient and safe solution is provided for the intestinal health of the pet.

The invention discloses a composition based on the synergistic effect of an SOD-LF covalent conjugate and neuromodulation and application of the composition in preparation of drugs for relieving anxiety and insomnia and improving sleep quality, and belongs to the technical field of biology. The preparation method comprises the following steps: preparing an SOD-LF covalent conjugate from lactoferrin and SOD; uniformly dispersing the SOD-LF covalent conjugate in a sodium alginate solution, spraying into a calcium chloride solution, standing, and filtering to obtain a sodium alginate coated SOD-LF covalent conjugate; and dispersing the sodium alginate coated SOD-LF covalent conjugate and fucoidin in a chitosan solution, adding sodium tripolyphosphate, stirring for reaction, and centrifugally collecting to obtain the composition based on the synergistic effect of the SOD-LF covalent conjugate and nerve regulation. According to the composition disclosed by the invention, the blood brain barrier transmittance is enhanced through the SOD-LF covalent conjugate, and the fucoidin is combined to regulate the intestinal flora-enteric brain axis to synergistically inhibit neuroinflammation and oxidative stress, so that anxiety and insomnia are improved.

The invention provides recombinant trichoderma reesei for producing lactoferrin and application of the recombinant trichoderma reesei, and belongs to the technical field of genetic engineering. Trichoderma reesei CJ2095 is used as a starting strain, disulfide bond isomerase is over-expressed in the trichoderma reesei CJ2095, a protein activating enzyme gene is knocked out, and the lactoferrin yield of the obtained recombinant trichoderma reesei reaches 306.31 mg / L. In addition, fermentation conditions of the recombinant trichoderma reesei are also explored, the recombinant trichoderma reesei can produce lactoferrin at high yield in a culture medium taking microcrystalline cellulose as a carbon source, and the concentration of bran and the concentration of Tween-80 can influence the yield of the lactoferrin. Therefore, in a culture medium which takes microcrystalline cellulose as a carbon source and contains bran and Tween-80, the recombinant trichoderma reesei disclosed by the invention can be fermented to generate 1349.5 mg / L of lactoferrin.

The invention belongs to the technical field of functional food, and particularly relates to a compound fermentation composition for regulating immunity and intestinal microecology as well as a preparation method and application of the compound fermentation composition. The invention provides a compound fermentation composition for regulating immunity and intestinal microecology. Comprising the following components: blueberry freeze-dried powder, an astragalus extract, a lycium ruthenicum extract, a day lily flower extract, grifola frondosa polysaccharide, schizochytrium limacinum powder, a red yeast rice extract, polygonatum polysaccharide, cordyceps militaris, xylooligosaccharide, yeast beta-glucan, lactoferrin, stevioside, a complex microbial inoculant and a complex enzyme. According to the compound fermentation composition disclosed by the invention, high-efficiency immunoregulation, long-acting intestinal balance and maximum component utilization are realized, and an efficient, stable and applicable solution is provided for regulating immunity and intestinal microecology.

The present invention discloses a nutritional paste and a preparation method thereof, and belongs to the technical field of animal feed preparation, the nutritional paste comprises a plurality of components, the eucommia ulmoides extract has good anti-inflammatory, analgesic and anti-stress effects, can relieve pain and inflammation after dogs and cats are sterilized, and can be used for preparing the nutritional paste for dogs and cats. And the astragalus extract (containing astragalus polysaccharide) has the effect of enhancing the immunity of the organism, so that the postoperative complications of dogs and cats after sterilization can be effectively prevented. The semen ziziphi spinosae, the gamma-aminobutyric acid and the tryptophan have the effects of calming and relieving restlessness, the lactoferrin can assist iron absorption and assist hematogenesis, meanwhile, the glutamine can promote wound healing, and the prebiotics can adjust intestinal flora imbalance caused by stress and use of antibiotics, so that postoperative gastrointestinal discomfort is avoided. The nutritional paste prepared by the invention can guarantee nutrient substances required by pet dogs and cats in the postoperative stage, promote wound healing of the pet dogs and cats after the operation, effectively relieve postoperative pain and restlessness, enhance the immunity of the organism and promote rapid recovery after the operation.

A use of a nutritional composition in promoting the bidirectional development of the gut and brain via the gut-brain axis. The nutritional composition comprises the following essential components: docosahexaenoic acid, eicosatetraenoic acid, galactooligosaccharide, 1,3-dioleic acid-2-palmitic acid triglyceride, lutein, nucleotide, lactoferrin, casein phosphopeptide, and probiotics. By combining and strengthening multiple functional active components, the gut health and brain cognitive development of the organism can be bidirectionally promoted by means of the microbiota-gut-brain axis. In particular, the present application can promote the regulation of flora in the gut of the organism, increase the abundance of beneficial bacteria, and decrease the abundance of harmful bacteria related to brain development diseases. The content of butyric acid in the gut can be increased, and the contents of acetic acid and propionic acid are decreased to promote brain and cognitive development.

The present invention discloses a method for inhibiting the digestion of lactoferrin in the human body. By preparing a complex of caffeine and lactoferrin and simulating in vitro digestion tests, it is found that caffeine increases the retention rate of lactoferrin in the oral cavity and intestine, while decreasing the retention rate in the stomach. This is beneficial for lactoferrin to bind with free oxygen radicals proximal to the pylorus of the small intestine, helping to protect the body from their harmful effects. The present invention prepares a complex of caffeine and lactoferrin, which can inhibit the digestion of lactoferrin in the human body, plays a positive role in enabling lactoferrin to better exert its functional characteristics, and at the same time, highly nutritious lactoferrin has broad application prospects in the development of functional foods.

The present application discloses a lactoferrin-modified liposome, a preparation method thereof and an application thereof. The liposome is loaded with Gastrodia elata active substances, and the Gastrodia elata active substances include barisonoside H, barisonoside K and barisonoside W. Through in vitro experiments and characterization, it is found that the liposome has a smoother drug release performance and drug loading performance for Gastrodia elata active substances after being modified with lactoferrin. The liposome has application prospects in related health fields such as hyperlipidemia and improvement of intestinal flora.