533 results about "Lactoferrin" patented technology

The invention discloses a nasal gel for treating allergic rhinitis and a preparation method thereof, and relates to the technical field of medicines. The nasal gel for treating allergic rhinitis is prepared from double-sensitive double-network drug-loaded gel microspheres and a temperature-sensitive gel material, the double-sensitive double-network drug-loaded gel microspheres are prepared from sodium alginate, a temperature-sensitive polymer, lactoferrin, a traditional Chinese medicine extracting solution and calcium chloride, the temperature-sensitive gel material is prepared from silk fibroin, carboxymethyl chitosan, K-carrageenan and genipin; the double-sensitive double-network drug-loading gel microspheres are loaded in the temperature-sensitive gel material, and after the temperature-sensitive gel material enters the nasal cavity, the temperature-sensitive gel material is quickly converted into gel to be adhered to nasal cilia, so that the removal of the double-sensitive double-network drug-loading gel microspheres by the motion of the nasal cilia is reduced, and the gel microspheres can better release a double-layer drug-loading system; the acting time of the medicine in the nasal cavity is prolonged, the bioavailability of the medicine is improved, the allergic rhinitis is effectively treated, and uncomfortable symptoms are relieved.

The invention discloses a preparation method and application of a lactoferrin-sodium alginate hydrogel system, and the preparation method of the lactoferrin-sodium alginate hydrogel system comprises the following steps: S1, preparing a lactoferrin subunit solution; s2, preparing a lactoferrin-active substance co-assembly; s3, purifying; s4, preparing a sodium alginate solution; and S5, preparing the lactoferrin-sodium alginate hydrogel loaded with the active substance. According to the method disclosed by the invention, small-molecule active substances are encapsulated on the basis that lactoferrin has a natural cage-shaped structure and a self-assembly characteristic, and lactoferrin is modified by utilizing good gel characteristic and gastrointestinal digestion resistance characteristic of sodium alginate, so that a lactoferrin-sodium alginate co-protected active substance hydrogel system is constructed; the constructed system shows obvious superiority in processing adaptability and bioaccessibility, and the problems of poor stability, low bioavailability and the like of natural small-molecule active substances in the food processing process are solved.

The invention relates to the technical field of nutritional supplements. A traditional fat reducing method mainly comprises dieting, high-intensity exercise, medicine weight losing and the like. However, dieting may lead to malnutrition; high-intensity sports are difficult to insist, and sports injury is easily caused; in order to solve the technical problems, the invention discloses a fat-reducing nutritional composition capable of improving metabolism, and the fat-reducing nutritional composition can be used for solving the technical problems of severe side effects such as tachycardia, insomnia and the like caused by medicine weight loss. The composition comprises the following components in parts by weight: 13-21 parts of a bitter gourd extract, 15-31 parts of a garcinia cambogia extract, 13-17 parts of oat fibers, 17-23 parts of whey protein powder, 7-9 parts of linseed oil, 6-14 parts of a green tea extract, 9-15 parts of a calcium supplement and 15-19 parts of a purple perilla seed extract, and can provide energy for a user, supplement immune globulin, lactoferrin and a calcium source, improve the immunity of the user and improve the immunity of the user. The body metabolism level and immunocompetence are improved, absorption of fat in digestive tracts can be effectively inhibited, storage of fat in the body is reduced, fat consumption is promoted, and the fat reducing effect is achieved.

The invention belongs to the field of nutrient substance research, and particularly relates to application of a nutrient composition to regulation of brain immunity. The invention provides application of a nutritional composition in preparation of food for regulating brain immunity. Wherein the nutritional composition comprises the following necessary components: lactoferrin and fucosyllactose, and in the nutritional composition, the mass ratio of the lactoferrin to the fucosyllactose is 1: (0.1-30). Research finds that after lactoferrin and fucosyllactose are combined according to a certain proportion, the lactoferrin and fucosyllactose have a synergistic effect of regulating brain immunity. Experimental data show that after the combination of lactoferrin and fucosyllactose is used for treating the BV2 cells, the trend that the BV2 cells are differentiated into M2 phenotype microglial cells is higher than the trend that the BV2 cells are differentiated into M1 phenotype microglial cells, and the amount of secreted inflammatory factors is obviously reduced.

The invention belongs to the technical field of biological medicines, and particularly relates to a postbiotic hydrogel for relieving alcoholic gastric ulcer as well as a preparation method and application thereof. The metagen hydrogel for relieving the alcoholic gastric ulcer, provided by the invention, is mainly prepared from metagen of rhamnobacter and modified lactoferrin; the lactobacillus rhamnosus is lactobacillus rhamnosus JM023. Tests prove that when the postbiotic hydrogel is applied to an alcoholic gastric ulcer rat model, the occurrence of gastric ulcer can be inhibited by inhibiting oxidative stress and inflammatory response and improving protection factor secretion; and the alcoholic gastric ulcer is relieved by regulating and controlling the expression of oxidation, inflammation, cell apoptosis and tissue repair related metabolic pathways and regulating gastrointestinal flora. The metagen hydrogel disclosed by the invention has the effect of effectively relieving gastric ulcer symptoms caused by alcohol, and is suitable for being developed and applied as a medicine or a health-care product for relieving alcoholic gastric ulcer.

The invention provides universal functional formula milk powder for cats and dogs and a preparation method thereof, and relates to the field of pet formula food. The formula milk powder consists of goat milk powder, colostrum powder, whey protein powder, lactoferrin powder, yolk powder, immune globulin powder, compound grease, compound minerals, compound vitamins, choline, taurine, compound prebiotics, compound probiotics, docosahexaenoic acid, arachidonic acid, casein phosphopeptides, L-glutamine and beta-galactosidase. And the goat milk powder, colostrum powder, whey protein powder, lactoferrin powder, yolk powder and immune globulin powder are subjected to low-temperature sterilization treatment by adopting an induced electric field or high-pressure microjet. The cat and dog formula milk powder overcomes the defects in the prior art, through compounding of basic nutritional ingredients and functional ingredients, a novel low-temperature sterilization technology is adopted for sterilization treatment, on the premise that the functionality of the formula milk powder is guaranteed, the nutritional value of the formula milk powder is reserved to a greater extent, and healthy growth and development of cats and dogs are promoted.

The present invention belongs to the field of biomedicine, and specifically relates to an antimicrobial peptide derived from lactoferrin and its application. The antimicrobial peptide derived from lactoferrin, whose amino acid sequence is shown in any one of SEQ ID NOs: 1 to 98, has a broad-spectrum antimicrobial effect, especially significant antibacterial effect against Pseudomonas putida, Rhodococcus erythropolis, Bacillus subtilis, Malassezia furfur, Cryptococcus neoformans, and Candida albicans, and has great application value.

The invention relates to the technical field of yak milk powder, and discloses yak milk powder helpful for promoting bone growth and development. Comprising the following specific raw materials: basic raw materials: raw yak milk, dried skim milk, cattle spleen peptide powder, colostrum powder, hydrolyzed yolk powder, phosphatidylserine, N-acetylneuraminic acid, colostrum basic protein, lactoferrin, docosahexaenoic acid, arachidonic acid, xanthophyll and choline; the polypeptide combination comprises Chinese yam peptide powder, poria cocos peptide powder, hawthorn peptide powder, endothelium corneum gigeriae galli peptide powder, walnut peptide and yak bone peptide. Through polypeptide combination of colostrum basic protein and composite mineral supplement, digestion and absorption of children to yak milk powder are promoted, various mineral substances needed by the body are supplemented, immunity is enhanced, bone growth is promoted, multivitamins needed by the body are supplemented by multivitamins, growth and development are promoted, bone growth is indirectly supported, and the yak milk powder has the effects of improving the immunity and improving the immunity. The requirements of children on the key nutrients in the bone growth and development stage are met.

The invention provides a pharmaceutical composition containing lactoferrin and application of the pharmaceutical composition in blood fat reduction. Specifically, the KGT-C polypeptide provided by the invention has a very strong effect of reducing the synthesis amount of cholesterol in cells, and after the KGT-C polypeptide is combined with lactoferrin, the generation of cholesterol and LDL can be remarkably reduced, so that the KGT-C polypeptide has a relatively good effect of treating hyperlipidemia.

The invention discloses an application of knockout of PEP4 and BAR1 genes in reduction of degradation of Kluyveromyces marxianus on lactoferrin. The invention provides application of inhibiting expression of PEP4 protein and BAR1 protein in any one of the following aspects: (A1) reducing degradation of Kluyveromyces marxianus on lactoferrin; and (A2) reducing the degradation of the fermentation supernatant of the kluyveromyces marxianus on lactoferrin. Experiments prove that after the encoding gene of the PEP4 protein and the encoding gene of the BAR1 protein are knocked out in yeast cells, the lactoferrin degradation amount can be reduced, the complete protein amount is remarkably increased, and the method has important significance on increasing the secretory expression yield of the lactoferrin in kluyveromyces marxianus.

The invention provides a milk powder composition containing breast milk oligosaccharide and easy to digest and absorb. The milk powder composition comprises the breast milk oligosaccharide, OPN, galactooligosaccharide, lactoferrin, alpha-whey protein, multivitamins, lactose, vegetable oil and compound minerals, wherein the weight ratio of the lactoferrin to the OPN to the breast milk oligosaccharide to the galactooligosaccharide is 1: (2-4): (2-50): (4-100). The composition can effectively recover and improve the intestinal barrier function caused by inflammatory factors, can be well absorbed by infants with poor digestion and absorption functions, reduces diarrhea and promotes skeletal development.

The present invention discloses the use of lactoferrin as a humectant in cosmetic or personal care products.

The invention belongs to the technical field of medical nano-materials, and particularly relates to a lactoferrin nano-material for sonodynamic regulation and control of blood-brain barrier permeability as well as a preparation method and application of the lactoferrin nano-material. The nanomaterial is nanoparticles LCe6 formed by self-assembly of lactoferrin and chlorin e6, can target brain microvascular endothelial cells, and can temporarily, reversibly and targeting open BBB by using the sonodynamic effect of the LCe6 in combination with transcranial ultrasonic stimulation, so that the accumulation of the nanoparticles LCe6 at a focus part is improved; in addition, the nanoparticle LCe6 also has a targeting property on brain tumor cells, the tumor cells can be accurately killed by utilizing the sonodynamic effect of the nanoparticle under secondary transcranial ultrasound, and the effect of sonodynamic treatment on brain glioma is exerted to the maximum extent; the nanoparticles LCe6 have excellent biocompatibility and low toxicity; according to the invention, the intracerebral delivery efficiency of the drug can be obviously improved, and a novel method for targeting open brain disease BBB is provided.

The invention relates to the technical field of infant milk powder formulas and equipment, in particular to milk powder with key nutrition gold matching synergistic interaction and sterile matching equipment thereof. Comprising the following components in parts by weight: 60 to 80 parts of high-oil whey powder, 10 to 20 parts of whole milk powder, 5 to 10 parts of lactose, 2 to 8 parts of whey protein powder, 1.5 to 3.5 parts of choline bitartrate, 1 to 5 parts of polyfructose, 0.3 to 0.7 part of taurine, 0.5 to 1 part of a compound functional nutrition enhancer, 0.05 to 0.1 part of compound nucleotide and 0.03 to 0.09 part of lactoferrin, according to the present invention, the milk source and the calcium, iron and zinc are scientifically proportioned, such that the milk powder can provide the basic nutrition from the milk source, and can supplement the specific nutrients through the strengthening components so as to achieve the double supplement effect, and provide the balanced and comprehensive nutrition support for the infants.

The invention provides a preparation method and application of nano protein, and the preparation method comprises the following steps: adopting lactoferrin as a carrier, reducing by using a reducing agent, stirring with DNA enzyme and a DNA chimeric agent, and obtaining the nano protein by using a nano precipitation method, the preparation method comprises the following steps: by taking lactoferrin as a nano-carrier, opening a folding structure of the lactoferrin by utilizing glutathione, then adding chloroquine and DNA enzyme, and finally, accelerating the reformation of a disulfide bond of the lactoferrin by stirring. On one hand, a DNA chimeric agent chloroquine is utilized to maintain the eDNA configuration in the biological membrane to be B type, and on the other hand, the mycobacterium tuberculosis biological membrane is efficiently disintegrated based on the enzymatic hydrolysis capability of DNA enzyme; the nano protein can efficiently grab iron ions at a focus part based on the iron chelation characteristic of the nano protein, so that bacteria die due to iron deficiency, and the bioavailability of DNA enzyme and the hydrolysis efficiency of a biological membrane can be improved to a great extent.

The invention discloses a preparation method of a lactoferrin-polyphenol nano compound with an intestinal immune function. The method comprises the following steps: firstly, moderately hydrolyzing lactoferrin under the action of compound protease, and optimizing a protein molecular structure; then, compounding with water-soluble polyphenol (such as chlorogenic acid or epicatechin) by utilizing a metal ion chelating technology, and inducing lactoferrin to be bridged and aggregated by Ca to form a compound; and then, carrying out ultrasonic homogenization treatment, and further controlling the particle size to enable the particle size to be distributed in a range of 100-300 nm. The obtained compound is subjected to nanofiltration concentration and electrostatic spray drying to obtain a shell-core nano compound with a stable structure, and nitrogen-filled packaging is adopted to prevent oxidation. The lactoferrin-polyphenol nano-composite prepared by the invention not only has good stability and intestinal immunity enhancement effect, but also has remarkable antibacterial activity, and can be widely applied to functional foods and nutritional health-care products.

The invention discloses a method for preparing low-bitterness micromolecular bovine colostrum protein peptide powder by adopting a multi-stage membrane separation and enzyme membrane coupling combined technology, and belongs to the technical field of food processing. The preparation method comprises the following steps: firstly, dividing a colostrum component into components to be hydrolyzed by adopting a multi-stage membrane separation technology, namely casein intercepted by ceramic membrane microfiltration and lactoglobulin and lactalbumin filtered by an ultrafiltration membrane, and non-hydrolytic components, namely immune globulin and lactoferrin; the preparation method comprises the following steps: firstly, separating bovine coloctrum, then deeply hydrolyzing the separated lactoglobulin, milky white and casein flocculate with the molecular weight of less than 50kDa by adopting an enzyme membrane coupling technology, mixing with unhydrolyzed immunoglobulin and lactoferrin, and freeze-drying, so that the peptide content in the treated bovine coloctrum is more than 40%, the peptide content of 200-1000Da is more than 30%, the peptide content of 200-1000Da is more than 30%, and the peptide content of 200-1000Da is more than 30%. The preparation method is simple, the preparation dispersion time is within 10 s, no obvious bitter taste exists, the retention rate of immune globulin in bovine coloctrum reaches 90% or above, and the preparation method is suitable for large-scale industrial popularization.

The invention discloses a lactoferrin component-containing allergic skin essence, which is characterized by comprising basic components, and the basic components comprise a lactoferrin compound, hyaluronic acid and an oat extract; an advanced component, wherein the advanced component comprises nicotinamide, a green tea extract and squalane; the finished product is prepared by mixing a basic component and any advanced component, and the finished product comprises a first-order finished product which is prepared from the basic component and the advanced component comprising nicotinamide; the second-order finished product is prepared from basic components and advanced components including nicotinamide and a green tea extract; the third-order finished product is prepared from basic components and advanced components including nicotinamide, a green tea extract and squalane. The invention relates to the technical field of skin essence. According to the allergy skin essence containing the lactoferrin component, stepped products suitable for different crowds are provided, the economic pressure of personnel is effectively reduced, and the use effect of the products is good.

The invention relates to the technical field of food processing, in particular to antioxidant nano collagen peptide powder and a preparation method thereof.The antioxidant nano collagen peptide powder is prepared by forming antioxidant micelles wrapping collagen peptide through collagen peptide liquid and a complex antioxidant, loading the antioxidant micelles on a porous carrier and then conducting cross-linking through transglutaminase; tea polyphenol and whey protein are covalently combined to prepare a composite antioxidant, rapid free radical neutralization, lactoferrin inhibition of an oxidation source and synergistic effect of collagen peptide self-antioxidant groups are provided, multistage barrier design is combined, oxygen diffusion is isolated through a micelle hydrophobic core, and the contact area of micelles and the outside is reduced through physical adsorption of a porous carrier, so that the anti-oxidation effect of the collagen peptide is improved. Finally, the micelles are fixed in the carrier by utilizing an enzyme cross-linked network, so that the antioxidant nano collagen peptide powder has efficient and long-acting antioxidant capacity.

The invention provides modified milk powder capable of promoting bone health as well as a preparation method and application of the modified milk powder. The total protein content of the modified milk powder is 17-35 g / 100 g, the fat content of the modified milk powder is 1-32 g / 100 g, and the calcium content of the modified milk powder is 0.8-2.1 g / 100 g. Moreover, the modified milk powder contains bovine coloctrum and lactoferrin, the mass ratio of the bovine coloctrum to the lactoferrin is (5-100): 1 on the basis of the dry matter content, and the content of the lactoferrin in the modified milk powder is 2-400 mg / 100 g. The bovine colostrum and the lactoferrin are combined for preparing the milk powder, have a synergistic effect on promoting osteoblasts and inhibiting osteoclast proliferation, and are beneficial to bone health.

The invention discloses a method for screening high-yield recombinant human lactoferrin through nucleic acid aptamer-assisted mutagenesis of a trichoderma reesei strain and application, and belongs to the technical field of microorganisms. The nucleic acid aptamer Lac-6a-FAM-BHQ1 capable of changing the fluorescence intensity according to the content of lactoferrin is constructed on the basis of the aptamer Lac-6a, and the nucleic acid aptamer can be used for rapidly selecting a mutant strain for producing high-titer recombinant human lactoferrin. The ARTP mutagenesis technology is combined with a nucleic acid aptamer auxiliary screening system, the mutant strain with the improved recombinant human lactoferrin yield is rapidly obtained from a large number of mutant strains, and the yield reaches 274.57 mg / L and is improved by 24.8% compared with that of an original strain. After the genes 111681 and 62716 are over-expressed in T. reesei, compared with a control strain, the yields of the recombinant human lactoferrin of the recombinant strain are respectively increased by 6.9% and 22.1%.

The invention provides a nutritional composition for promoting children to grow tall and application of the nutritional composition. The invention firstly provides a composition which comprises zinc, lactoferrin, bovine colostrum and yeast beta-glucan, and the mass ratio of the zinc to the lactoferrin to the bovine colostrum to the yeast beta-glucan is (1.5-12): (16-800): (95-480): (14-75) according to the dry matter amount. The invention also provides a food containing the composition, and an application of the composition or the food in preparation of a product for promoting children to grow tall.

The invention discloses infant formula milk powder with high-activity immune protein and a preparation method of the infant formula milk powder, and belongs to the technical field of preparation of dairy products. The preparation method comprises the following steps: degreasing and degerming fresh milk, transferring lactoferrin phase combination, enriching lactoferrin to a whey phase through filtration of a microfiltration membrane, further desalting and concentrating the whey phase through ultrafiltration and electrodialysis, and forming active whey rich in lactoferrin after sterile concentration; the trapped fluid filtered by the microfiltration membrane is subjected to milk killing (primary sterilization), then is blended with other nutrients, is homogenized, is subjected to material killing (secondary sterilization) and is concentrated, and finally, the treated trapped fluid and the active whey which is subjected to sterile concentration and is rich in lactoferrin are mixed on line and then are subjected to evaporation concentration and spray drying; the infant formula milk powder rich in active whey protein with high lactoferrin content is obtained.

The invention discloses a lactoferrin and antibacterial peptide bifunctional hydrogel dressing as well as a preparation method and application thereof. The hydrogel dressing is prepared by mixing lactoferrin, antibacterial peptide NZ2114, a magnesium lithium silicate gel matrix and water. The hydrogel dressing provided by the invention has the following advantages: 1) the hydrogel dressing is simple to prepare, low in cost and suitable for batch production; 2) the gel is convenient to apply, has a moisturizing effect, is long in residence time, good in biocompatibility, small in irritation, safe and reliable, or is accurately injected to the wound, moisturizes, slowly releases and efficiently improves the compliance of the body, and 3) the gel is good in antibacterial and anti-inflammatory effects, good in biocompatibility, free of irritation and capable of promoting wound healing, and only needs to be smeared on the surface of the wound.

The invention belongs to the technical field of biological medicines, and particularly relates to application of bifidobacterium breve and lactoferrin in preparation of products for regulating intestinal flora or resisting bacterial infection. The invention provides application of Bifidobacterium breve HH079 with the preservation number of GDMCC No: 64216 and lactoferrin in preparation of a product for regulating intestinal flora or a product for resisting bacterial infection, and the Bifidobacterium breve HH079 and the lactoferrin are combined and synergistically used for resisting the bacterial infection. The composition can reverse weight loss, colon shortening, bacterial load increase and colon epithelium injury caused by bacterial infection, can significantly inhibit the level of proinflammatory cytokines, promote the expression of anti-inflammatory cytokines, can significantly increase the concentration of acetic acid and propionic acid in excrement of infected mice, can generate positive regulation on intestinal flora, and can inhibit bacterial infection. Good application prospects are shown in regulation of intestinal flora and bacterial infection resistance.

The invention discloses a nutritional composition beneficial to pet health and pet food containing the nutritional composition. The nutritional composition comprises beta-glucan, plasma protein and lactoferrin. Wherein the mass ratio of the beta-glucan to the plasma protein to the lactoferrin is (0.055 to 0.275) to (1.56 to 4.68) to (0.00095 to 0.0285). The nutritional composition beneficial to pet health provided by the invention is used for improving intestinal health of pets and improving digestive ability; the immunity of the pets can be improved, and the hair health of the pets is improved.

The present invention discloses a lactoferrin composition and its application for improving immunity by regulating macrophage immune activity, and belongs to the technical field of functional compositions. The present invention obtains a lactoferrin composition with regulating macrophage immune activity by compounding lactoferrin, α-lactalbumin, N-acetylneuraminic acid and yeast β-glucan in a specific ratio, the raw material of the composition is easy to obtain, and four components work together to significantly enhance its phagocytic activity without changing the activity of macrophages, regulate the polarization state of macrophages in inflammatory response, inhibit macrophages from polarizing to pro-inflammatory M1 type, and promote macrophages to polarize to anti-inflammatory M2 type; At the same time, the composition can also enhance the antigen presentation ability of macrophages and the activation of immune T cells, and improve body immunity. Therefore, the composition provided by the present invention can be used to prepare health products, food or medicines, etc. that improve immunity.

The invention relates to the technical field of fermentation preparation devices, and particularly discloses a lactoferrin yoghourt fermentation preparation device and method.The lactoferrin yoghourt fermentation preparation device comprises a rack and a fermentation tank fixedly installed in the rack, a transfer tank is fixedly installed on the outer side of the fermentation tank, a material pouring and defoaming mechanism is arranged in the fermentation tank, and a stirring mechanism is arranged at the bottom of the material pouring and defoaming mechanism; a follow-up material pumping and pressing mechanism is arranged between the transfer tank and the fermentation tank, a fluid channel of a material pressing cone is composed of three sections, each section is responsible for treating bubbles of different sizes, and a bottom strong shearing section rapidly breaks small bubbles through high shearing force and shears large bubbles into multiple medium or small bubbles; the middle pressure-adaptive section helps bubbles with different sizes to gradually float through moderate pressure difference and shearing force; and the top pressure reduction section ensures that all residual bubbles can smoothly emerge from the surface and are discharged through a low-pressure environment.

The invention discloses a protective agent as well as a preparation method and application thereof. The protective agent is prepared from inulin, fructo-oligosaccharide and erythritol. According to the protective agent provided by the invention, the inulin, the fructo-oligosaccharide and the erythritol are compounded to form a mixed protective agent, so that the heat resistance of functional protein in the bovine colostrum processing process can be improved, and the protective agent has a good protective effect on immune globulin (Ig-G) and lactoferrin (LF) in the processing process. Moreover, the three components all have probiotic effects, so that the development of high-value bovine coloctrum functional products is facilitated, and the comprehensive benefits of the bovine coloctrum are improved.

Topical compositions comprise at least one hydrophilic antioxidant, wherein the at least one hydrophilic antioxidant comprises sodium ascorbate; a hydrophobic antioxidant; and an enzymatic antioxidant. Topical compositions according to the present disclosure may be used to treat skin (e.g, by reducing the effects of UV exposure or aging on skin), to stimulate or preserve elastogenesis in skin, to stimulate or preserve collagenesis in skin, to stimulate or preserve production of elastin and collagen in skin, to reduce the amount of ROS in skin, to improve the appearance of skin, or to reduce the appearance of fine lines, wrinkles, or crepiness in skin. Topical compositions comprising sodium ascorbate and lactoferrin synergistically enhance elastogenesis in skin, while preserving CD44 expression in skin, relative to compositions comprising sodium ascorbate without lactoferrin.