The invention discloses a feed for piglets with reduced serum lipopolysaccharide level and its preparation method and application, which solves the problem that the disease resistance and immunity of piglets cannot be improved in the prior art. The invention is made of the following components according to a certain weight ratio: corn, puffed corn, broken rice, puffed soybean, fermented soybean meal, fish meal, soybean meal, soybean oil, stone powder, calcium hydrogen phosphate, sodium chloride, choline chloride, betaine , lysine, methionine, threonine, tryptophan, benzoic acid, phytase, compound enzyme, trace element premix, vitamin premix, enramycin premix, arginine, glutamine Amide, proline, astragalus polysaccharide, eleuthero polysaccharide, curcumin, chitosan oligosaccharide, probiotics, yeast cell wall extract. The composition of the invention is scientific, the compatibility is reasonable, each component acts together, can provide balanced and easily digestible nutrients for the growth and development of piglets, and effectively reduce the level of serum lipopolysaccharide of piglets.