311 results about "Animal breeding" patented technology

The invention relates to the field of animal breeding, and discloses a pig beta defensin compound preparation which comprises pig beta defensin PBD1, PBD2 and PBD114, and the weight ratio of the PBD1 to the PBD2 to the PBD114 is 1: 1: 1. The porcine beta defensin PBD1, the porcine beta defensin PBD2 and the porcine beta defensin PBD114 are recombinant proteins, and are respectively expressed in escherichia coli BL21 (DE3) PLysS competent cells by a pET-32a expression vector. Through the synergistic effect of the defensins PBD1, PBD2 and PBD114, the minimum inhibitory concentration (MIC) of the EPEC is reduced to 18.75 mu g / mL from 75 mu g / mL of single defensins, and is reduced by 75%; within 24 hours, the bacteriostasis rate of the compound preparation reaches 90%, and the inhibition effect on EPEC is remarkably improved.

The invention belongs to the technical field of the Internet of Things, and particularly relates to a breeding weighing management system based on the Internet of Things and an AI visual technology. Comprising a multi-source data set acquisition module, an animal identity identification data set determination module, an animal updated weight table output module, a weighing management report output module, an animal breeding risk analysis module and a breeding weighing management decision suggestion generation module. The problems that traditional non-contact weighing precision and adaptability are insufficient, existing AI models mostly aim at single species, the generalization ability of the models is limited, multi-source data fusion and standardization are insufficient, and robustness of the AI models has defects are solved, the efficiency and precision bottlenecks of manual operation are broken through, and the accuracy of the AI models is improved. The requirements of large-scale breeding for data instantaneity and management refinement can be met, and the breeding industry is promoted to be upgraded to digitization and intellectualization.

The invention belongs to the technical field of animal breeding, and particularly relates to application of a detection reagent of an SNP (Single Nucleotide Polymorphism) molecular marker related to pork tenderness. An SNP site exists at the 232bp position from the 5'end of the sequence, the polymorphism is G / A, three genotypes of GG, GA and AA are shown, G allele is dominant allele, and the meat tenderness of a GG type individual is obviously higher than that of an AA type individual. The invention further develops a reagent for detecting the SNP molecular marker, the reagent can be used for pig assisted breeding, pig breeding or pork tenderness identification, and a method for identifying the pork tenderness is developed, which is beneficial to accelerating genetic breeding and early-stage seed selection of boars with high-quality meat traits.

The invention belongs to the technical field of animal breeding, and particularly relates to a molecular marker closely linked with beef quality characters, a primer pair, a detection reagent and application. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, the nucleotide shown as SEQ ID NO.1 has G / A polymorphism at the 831th bp, when the genotype of the 831th site from the 5'end of the molecular marker is GG, the meat color brightness and intramuscular fat content of an individual are higher than those of a GA genotype individual, and the cooking loss of the individual is lower than that of the GA genotype individual, and the molecular marker can be used for selective breeding of high-quality beef cattle.

The invention discloses a preparation method and application of an areca total alkali extract. The areca total alkali is calculated by arecoline, arecoline, demethylated arecoline and demethylated arecoline. The preparation method comprises the following steps: carrying out water extraction on areca catechu, purifying an extracting solution through cationic resin, concentrating an eluent, regulating acid, carrying out suction filtration, and drying to obtain the areca catechu total alkali extract. According to the preparation method, high-purity alkaloid can be obtained from areca nuts, the process is simple, convenient and stable, the cost is low, the preparation method can be widely applied to industrial production, and the preparation method has the effects of improving rumen fermentation characteristics, protecting rumen health, improving nutrient digestibility, promoting animal growth and improving animal production performance when applied to economic animals.

The invention belongs to the technical field of animal breeding, and particularly relates to application of a detection reagent of an SNP (Single Nucleotide Polymorphism) molecular marker related to pork quality traits. Basic groups at 208bp and 217bp from the 5'end of the sequence have synchronous C / T mutation, the sequence shows three genotypes of CC, CT or TT, T allele is dominant allele, and the intramuscular fat content of the TT type pig is greater than that of the CC type pig. The invention further develops a reagent for detecting the SNP molecular marker, the reagent can be used for pig assisted breeding, pig breeding, pork quality character identification or pork quality character screening, and a method for identifying the intramuscular fat content of pork and screening pork with rich intramuscular fat is developed.

The present disclosure relates generally to methods and test kits for identifying SNPs associated with desirable meat eating quality traits in ovine animals. In particular, the present disclosure relates to a SNP-based diagnostic test and method for identifying ovine animals with desirable fat melting point (FMP), intramuscular fat (IMF) and omega-3 long chain polyunsaturated fatty acids (n-3 LC PUFAs) which are characteristic of Australian White sheep or Lamb (e.g., Tattykeel Australian White Lamb), and the use of those tests and methods in animal breeding programs.

The utility model discloses a rearing type double-layer experimental animal isolator, and aims to solve the problems that a traditional single-layer rearing isolator is small in animal breeding quantity, large in occupied area and high in labor intensity due to manual excrement removal. The isolator adopts a double-layer box body structure and comprises an upper-layer box body and a lower-layer box body which are communicated with each other, and excrement receiving devices and air conditioning systems are arranged at the bottoms of the isolator. The excrement receiving device is composed of an excrement receiving disc and an excrement scraping device, and excrement is automatically cleaned to the excrement collecting box through an excrement scraping plate. A feces discharge device is arranged in the feces collection box, and a stirring paddle and an electric valve are matched with a concentric-square-shaped bent pipe, so that feces can be discharged in time, and manual intervention is reduced. The air conditioning system adopts an independent air supply system and an air exhaust system, an air supply outlet and an air exhaust outlet are optimally designed to ensure uniform airflow, the air supply outlet and the air exhaust outlet are diagonally arranged, an upper-supply and lower-return air supply mode is adopted, the air supply outlet adopts a static pressure pore plate to supply air, and the air conditioning system has the characteristics of uniform air supply, low noise and small blowing feeling to animals, and provides a good raising environment for the animals.

The invention belongs to the technical field of animal breeding, and particularly relates to a molecular marker closely linked with beef intramuscular fat traits, a primer pair, a kit and application. Nucleotide sequences of the molecular marker are shown as SEQ ID NO.1 and SEQ ID NO.2, the molecular marker has T / C polymorphism from the 76th basic group of the 5'end, and when the genotype of the locus is TC, the intramuscular fat content of beef of an individual is higher than that of the individual with the genotype of TT. The beef cattle with the high intramuscular fat content character can be screened or cultivated by utilizing the molecular marker.

The invention belongs to the technical field of aquatic animal breeding, and particularly relates to a grass carp parent salinity tolerance related molecular marker suitable for fry salinity domestication and application of the grass carp parent salinity tolerance related molecular marker in grass carp salt tolerance breeding. The nucleotide sequence of the SNP molecular marker related to the salinity tolerance character of the grass carp is as shown in SEQ ID NO: 1; a / C polymorphism exists at the 111st site in the DNA fragment shown in the nucleotide sequence, and when the genotype of the SNP molecular marker is CC or AC, the to-be-detected sample is the salinity-tolerant grass carp; when the genotype of the SNP molecular marker is AA, the to-be-detected sample is the salinity-sensitive grass carp. The SNP molecular marker closely related to the salt tolerance character is screened through a salinity tolerance test. Therefore, the molecular marker provided by the invention can be used for carrying out breeding work and detection work of the salt-tolerant grass carp.

The invention relates to a genome-based animal hybridization effect analysis and prediction method and application thereof, and the method comprises hybridization effect analysis, and the hybridization effect analysis specifically comprises genotype combination distinguishing, phenotype optimal value determining, each character, and the like. And quantifying the deviation between the phenotypic expression of each genotype combination and the phenotypic optimal value by using the phenotypic distance, calculating the size of the hybridization effect of each genotype for each character, obtaining a lambda parameter, and searching a hybridization dominant region by using genomic differentiation data. The method is applied to animal breeding, the quantitative relation between the genomic differentiation degree and the hybridization effect is established for the first time, the genomic area sensitive to hybridization is recognized by analyzing the hybridization effect difference of different genomic areas, the hybridization effect can be accurately predicted, long-term guidance is provided for follow-up multi-generation breeding, and the method is suitable for large-scale popularization and application. The number of actual hybridization tests is remarkably reduced, the breeding cost and time are saved, and the breeding process is accelerated.

The utility model relates to the field of animal breeding, in particular to a pig manure solid-liquid separation device convenient for blanking for a pig farm. Comprising a separation box, the side end of the separation box is fixedly connected with a stirring and conveying assembly, the inner wall of the separation box is provided with a smashing assembly, and the inner wall of the separation box is fixedly connected with a solid-liquid separation filter plate; the separation mechanism and the separation mechanism used for separating and filtering the excrement are arranged in the separation box, when the device is used for processing the excrement, the excrement is thrown in from the upper side of the separation box, the excrement entering the separation box is smashed through the smashing assembly, and the smashed excrement on the upper side falls into the pushing assembly downwards; the crushed excrement on the upper side is filtered through the pushing assembly and the attaching assembly, and at the moment, the separated soft and small pieces of excrement downwards fall into a solid-liquid separation filter plate to separate liquid from solid, so that subsequent discharging is facilitated.

The present invention relates to the technical field of animal breeding, and particularly relates to an SNP molecular marker related to chicken feed conversion rate and its application. The SNP molecular marker includes one or more of rs15450437, rs314248376 or rs15702068; rs15450437 is located at 143623538 bp on chicken chromosome 1, and the polymorphism is T / C; rs314248376 is located at 52556471 bp on chicken chromosome 3, and the polymorphism is C / T; rs15702068 is located at 12271457 bp on chicken chromosome 13, and the polymorphism is A / G. The present invention provides SNP molecular markers related to chicken feed conversion rate. Based on the polymorphism detection results, the feed conversion rate of chickens can be judged. These SNP molecular markers are further applied to breed chicken varieties with different feed conversion rates, which has important value.

The invention belongs to the technical field of vitamin coating and animal breeding, and particularly relates to coated 25-hydroxyvitamin D3, a preparation method thereof and application of the coated 25-hydroxyvitamin D3 in improving the lactation performance and milk quality of ruminants. The 25-hydroxyvitamin D3 disclosed by the invention can regulate and control the release rate of the 25-hydroxyvitamin D3 in rumen and small intestines, so that the 25-hydroxyvitamin D3 can reach the small intestines to be absorbed to the greatest extent, the nutrition regulation and control function of the 25-hydroxyvitamin D3 is fully played, the lactation performance of dairy cows is improved, meanwhile, the lipid metabolism of ruminants can be regulated and controlled, and the lactose content in milk can be increased. The test result shows that by adding the coated 25-hydroxyvitamin D3 into the basic ration of the dairy cow, the milk yield of the dairy cow can be increased, and meanwhile, the fat content and the lactose content in the milk can be remarkably increased. Therefore, the coated 25-hydroxyvitamin D3 can improve the feed efficiency, reduce the feeding cost and improve the production benefit.

1. The name of the design product: feeding trough. 2. The use of the design product: automatic feeding trough device for cattle, sheep and other animal breeding. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

The invention belongs to the technical field of animal breeding, and particularly relates to application of a detection reagent of a molecular marker related to the hair length character of a down producing goat. The molecular marker is located at 155, 344, 701-155, 346 and 600 positions on a No.1 chromosome of a goat genome, and the nucleotide sequence of the molecular marker is as shown in SEQ ID NO. 1. It is found that the wool length of an individual with a normal molecular marker area is larger than that of an individual with a deletion molecular marker area, and therefore the cashmere goat variety with the large wool length can be bred by detecting deletion mutation of the molecular marker on the chromosome 1 of the goat genome.

The invention relates to the technical field of animal breeding, in particular to a molecular marker related to the width of a seventh web of a bee and application of the molecular marker in molecular marker-assisted breeding of the bee. The molecular marker comprises a nucleotide sequence as shown in SEQ ID NO.1, the 194th site of the nucleotide sequence is a polymorphic site, and the polymorphism is A or C. The molecular marker has a nucleotide sequence as shown in SEQ ID NO.1. According to the invention, an SNP site related to the seventh web width character of bees is screened, and a corresponding molecular marker is developed. The molecular marker provided by the invention can be used for molecular marker-assisted breeding of bees and breeding of high-quality bee varieties.

The invention discloses application of spermidine in preparation of a preparation for improving fertility of female mammals, and belongs to the technical field of reproductive biology. According to the invention, spermidine with different concentrations is introduced into mice, and the effect of spermidine on ovarian hormone secretion, ovarian reserve level and follicle number of female mammals is determined in a diet intake manner, so that the fertility of female animals is improved; it is found that spermidine can effectively improve the ovarian function, increase the secretion level of E2, P4, LH and AMH hormones closely related to the ovarian function, reduce the number of atresia follicles of the ovarian and increase the litter birth number of female mice by 1-2, and the spermidine has good animal breeding and livestock raising application prospects.

The invention relates to the technical field of animal breeding, in particular to a method for breeding black sheep. The method comprises the following steps: introducing an expression cassette into a cell to obtain a transgenic cell; and carrying out somatic cell nuclear transplantation cloning by adopting the transgenic cells to obtain the black sheep. The expression cassette comprises an intron of PK5 and beta-globin, and an EDN3 gene, the PK5 comprises a nucleotide sequence as shown in SEQ ID NO. 2; and the EDN3 gene comprises a nucleotide sequence as shown in SEQ ID NO. 4. A specific expression cassette and a carrier are researched, the black sheep of which the skin, hair, tongue, oral mucosa, eye white mucosa, epididymis and ears all become black can be prepared through somatic cell cloning on the basis of cells edited by the carrier, and important application value and economic value are achieved.

The invention provides a TRPM8 protein mutant and application thereof, relative to an amino acid sequence of a wild type TRPM8 protein, the TRPM8 protein mutant comprises mutation at one or more positions selected from the 825th site, the 864th site, the 891th site, the 897th site, the 915th site, the 917th site, the 925th site, the 927th site, the 928th site, the 932th site, the 945th site, the 946th site and the 948th site. The invention also provides a separated nucleic acid which is used for coding the TRPM8 protein mutant. The invention also provides a vector containing the isolated nucleic acid and a genetically recombinant porcine fetal fibroblast. According to the invention, a key site which can effectively reduce the cold activation level of the pig TRPM8 without influencing other activation functions is screened, and a new material is provided for animal breeding improvement.

The invention discloses a molecular marker primer for identifying genetic sex of pike fish and application thereof, and relates to the technical field of aquatic animal breeding. The invention provides a molecular marker primer for identifying genetic sex of pike fish and application of the molecular marker primer. The molecular marker primer for identifying the genetic sex of the pike comprises a molecular marker specific primer and an auxiliary molecular marker primer, and the molecular marker primer for identifying the genetic sex of the pike is applied to all-female breeding of the pike. Compared with the prior art, the molecular marker with extremely high correlation with the genetic sex of the pike vibrio molitor and the genetic sex of the pike vibrio molitor is adopted, the difference between the molecular marker and the prior art is that the molecular marker is universal in genetic sex identification of the pike vibrio molitor and the genetic sex of the pike vibrio molitor, and in addition, the identification accuracy of the molecular marker reaches up to 100% and is obviously superior to that of the prior art. Aiming at the problems of high all-female breeding cost and low efficiency caused by shortage of a dog fish pseudo-male fish screening technology, the invention provides a dog fish genetic sex identification marker and a set of efficient and convenient dog fish all-female breeding method which is formed by taking the dog fish genetic sex identification marker as a core, so that the development of the dog fish breeding industry is effectively promoted.

The utility model belongs to the field of animal breeding, and particularly relates to an amphibian automatic breeding modularization system. Comprising a box body, the box body comprises a bottom plate fixedly arranged at the bottom, a supporting plate is transversely and vertically arranged at the top of the bottom plate, a feeding area is arranged on one side of the supporting plate and abuts against the inner side of one side wall of the box body, and an automatic feeding device is arranged on the outer side of the side wall, abutting against the feeding area, of the box body in a penetrating mode; a top plate of the feeding area can be automatically flushed to prevent food residues from remaining, and the other side of the supporting plate is provided with a water area for animals to survive; a top cover is detachably arranged at the top of the box body, a control unit is arranged at the bottom of the top cover, and a full-spectrum illuminating lamp, a camera, an ultraviolet lamp and a regulation and control unit are detachably arranged at the bottom of the top cover and used for monitoring and adjusting the internal environment.

1. The name of this design product: Animal breeding feeder (group animal breeding). 2. Purpose of this design product: used for feeding feed. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1.

The invention belongs to the technical field of breeding of aquatic animals, and particularly relates to a breeding method for breeding XX pseudo male fishes by using Chrysophila multistriata. The invention relates to a breeding method for breeding XX pseudo-male fishes by using Christia polyrrhiza, which comprises the following steps of: feeding a feed containing an aromatase inhibitor to Christia polyrrhiza fry with proper day age for induction to obtain pseudo-male fishes with physiological male property and genetic female property; the method comprises the following steps: performing PIT living body electronic marking, then collecting a tail fin tissue sample, performing PCR detection by utilizing a genetic sex specific molecular marker of the Chrysophila polyrrhiza, and screening out pseudo male individuals with female genetic sex and male physiological sex; the obtained pseudo male fish individuals serve as male parents, normal genetic female fishes serve as female parents, intensive cultivation and artificial spawning induction are conducted, and fertilized eggs are obtained; the obtained fertilized eggs are collected and hatched, and pseudo male fishes with all genotypes being XX are obtained. According to the method, the genetic all-female rate of the breeding offspring reaches 100%, and the production purity of the offspring seeds is greatly improved.

The application discloses an amphibian artificial bionical natural breeding device and a breeding method, and belongs to the technical field of breeding devices. The amphibian artificial bionical natural breeding device comprises a breeding pond, and the breeding pond comprises a pond body. A breeding area is arranged in the middle of the pond body. Inclined climbing areas are arranged on the two sides of the breeding area. The climbing areas are connected with the breeding area through transition areas. A plurality of breeding frames for animal breeding are arranged between the climbing areas and the transition areas along the length direction of the pond body. Overflow holes communicating with the breeding area are arranged at the bottom of the breeding frames. Climbing passages for adult animals to return to the road surface are arranged between the breeding frames. The breeding area is internally provided with an avoiding structure for larvae to avoid. The amphibian artificial bionical natural breeding device and the breeding method can solve the problems of high cost of artificial propagation of the existing Tianhai spiny salamander and poor environmental adaptability of adults.

The invention belongs to the technical field of animal breeding, and discloses an application of N-methyltyramine hydrochloride in a breeding animal feed and the feed, the N-methyltyramine hydrochloride is added into the breeding animal feed in a mixed or mixed granulation form, and preferably, 45g of N-methyltyramine hydrochloride is added into each ton of compound feed. The N-methyltyramine hydrochloride is chemically synthesized by taking an effective stomach-invigorating component N-methyltyramine in the traditional Chinese herbal medicine immature bitter orange as a technical basis. When the N-methyltyramine hydrochloride is added into the feed for the bred animals in a proper proportion, the secretion of endogenous enzyme of the bred animals can be promoted, so that the feed reward of the bred animals is improved.

The application relates to the field of animal breeding, in particular to a SV molecular marker of a pig rib number character and application. Specific primer pairs for analyzing the sites are provided, so that early accurate breeding of pigs is realized. The technical scheme has the advantages of simple operation, low cost, accurate detection and the like, and has important application value in molecular accurate breeding of the pig rib number character.

The invention relates to the technical field of microorganisms, in particular to Weissella fusiformis JWC120 with a function of inhibiting common harmful bacteria in animal breeding and application of the Weissella fusiformis JWC120. The Weissella fusiformis JWC120 with the function of inhibiting common harmful bacteria in animal breeding, provided by the invention, has the preservation number of CGMCC No.31945, and can effectively inhibit common escherichia coli, salmonella, staphylococcus aureus and shigella in animal breeding; a bacteriocin crude extract of the strain has a remarkable inhibition effect on common staphylococcus aureus in animal breeding, and has a good application prospect in animal disease prevention and treatment and development of livestock and poultry breeding health-care products.

The application discloses a snowflake meat sheep breeding method and relates to the technical field of animal breeding. The breeding method comprises the following steps: taking a hollow sheep as a female parent, taking an Australian cold sheep male sheep as a male parent, carrying out crossbreeding, and obtaining an Australian cold hollow female sheep group; taking an Australian cold male sheep as a male parent, taking an Australian cold hollow female sheep as a female parent, carrying out crossbreeding, and obtaining a Yellow River estuary meat sheep group; and carrying out crossbreeding and fixing of the male sheep and the female sheep in the Yellow River estuary meat sheep group. The snowflake meat sheep breeding method can improve the reproduction rate, the stress resistance, the growth speed, the carcass quality and the meat quality of the snowflake meat sheep, solves the snowflake variety demand of the development of the meat sheep industry in China, and has good popularization value.

The utility model discloses an animal feeding device, which belongs to the technical field of animal feeding and comprises a cage body, side fences are fixedly mounted on the periphery of the cage body, a hollow bottom fence is fixedly mounted at the bottom of the cage body, a fixing shell is fixedly mounted at the bottom of the cage body, and the side fences are fixedly mounted on the bottom of the cage body. The bottom of an inner cavity of the fixing shell is slidably connected with a collecting box, and the surface of the bottom fence is slidably connected with a cleaning ring. Through the arrangement of the drying assembly, excrement on the surface of the bottom fence can be dried, after the excrement is dried, a user can drive the cleaning ring to reciprocate on the surface of the bottom fence through the driving assembly, and the excrement on the surface of the bottom fence is scraped and cleaned. And the excrement on the surface of the bottom fence can be scraped and cleaned after being dried, so that a certain amount of excrement is prevented from remaining on the surface of the bottom fence in the excrement scraping and cleaning process.