13 results about "Grass carp" patented technology

This invention discloses a method for co-culturing giant freshwater prawns (Macrobrachium rosenbergii) with shrimp, grass, and fish based on probiotic regulation, and its application. This invention constructs a three-dimensional ecological system with shrimp as the main body, grass as the protector, and fish as a supplement. Elodea and water spinach purify the water, silver carp and crucian carp decompose uneaten feed and feces, and a compound microbial preparation of Lactococcus gasseri C6a2 regulates water quality, while a biological bottom conditioner improves the bottom sediment, achieving precise ecological regulation of the aquaculture environment. This invention does not rely on chemical agents, effectively inhibits ammonia nitrogen accumulation, reduces disease incidence, and significantly improves the survival rate and size of giant freshwater prawns, achieving a win-win situation for both ecological and economic benefits.

The application relates to the technical field of aquaculture, in particular to a composition, a feed additive, a feeding method and application. Embodiments disclose a composition composed of cinnamyl aldehyde, thymol, carvacrol and lauric acid monoglyceride, which is used for feeding Chinese soft-shelled turtles and grass carp, can effectively promote the growth of the Chinese soft-shelled turtles and the grass carp, improve the daily weight gain of livestock and poultry, improve the feed conversion rate, improve the meat quality and the protein content, improve the immune capacity of the aquaculture animals, and has the characteristics of easy availability of raw materials and low cost.

The present application relates to a kind of grass carp circulating water aquaculture tail water comprehensive treatment system based on MABR and its use method, it relates to aquaculture water treatment technical field.Processing system is sequentially connected physical filtration unit, biological filtration unit, MABR processing unit, disinfection unit and reuse unit, air supply unit is separately arranged;Use method is to apply MABR technology to biological filtration unit after, before disinfection unit, utilize physical filtration unit to remove large particle impurities and part of suspended solids in water body, the microorganism attached to biological filter material in biological filter carries out biological degradation to organic matter, ammonia nitrogen and other pollutants in water body, the microorganism attached to MABR membrane in MABR processing unit carries out depth degradation to the pollutants in water body, again, to the water body after treatment ultraviolet disinfection is killed pathogenic microorganism, subsequent water body oxygenation recycling or discharge is discharged.The present application has integrated technology removal effect, good stability, save external carbon source and other advantages.

The invention discloses a grass carp high-density whole genome 50K SNP (Single Nucleotide Polymorphism) chip and application thereof. Genetic typing objects of the chip comprise 49 and 635 SNP sites on the whole genome of the grass carp, the SNP sites cover 23835 genes of the grass carp, and the positions and genotypes of the SNP sites on the genome (PRJNA1368393) of the grass carp are shown in the specification table 1. The SNP is derived from re-sequencing results of 351 samples, is rich in gene region sites, high in typing accuracy and high in repeatability, is an accurate and efficient molecular breeding chip, can be widely applied to application scenes of grass carp germplasm resource identification, genetic diversity evaluation, target character gene mining and whole genome selective breeding, and has a wide application prospect. The method has the advantage of low detection cost and is beneficial to further improving the grass carp breeding efficiency.

The application belongs to the technical field of biology, and particularly relates to a grass carp reovirus (GCRV-Ⅱ type) subunit recombinant vaccine, a preparation method and application. 274‑367 , VP5 105‑336 , NS38 143‑338 sequence fragments, and the three sequence fragments are recombined and expressed in a prokaryotic expression vector to prepare the subunit recombinant vaccine. The recombinant vaccine has high safety, strong activity and broad spectrum, and has good application potential.

The application relates to the technical field of biology, and particularly relates to a kind of N 6 ,N 6 - Dimethyladenosine and its application. (1) The application locks the key metabolite N 6 ,N 6 - Dimethyladenosine with significant anti-GCRV activity through metabolomics. Experiments prove that the metabolite not only significantly reduces virus load at the in vivo level, but also extremely significantly down-regulates the expression of VP7 at the cell level, thereby inhibiting the assembly and replication of GCRV from the source, and having a clear anti-virus molecular mechanism. (2) The application discloses a new biological function of N 6 ,N 6 - Dimethyladenosine in the field of aquatic anti-virus, and proves that the metabolite can be used as an efficient anti-virus active molecule to inhibit GCRV replication. The application provides important theoretical support and factual basis for developing new, green and safe anti-grass carp hemorrhagic disease drugs and feed additives.

This invention relates to the fields of molecular biology and biomedicine, specifically to a method and application of recombinant methods for the grass carp IL-21 optimized gene, its recombinant protein, and plasmids. The recombinant protein of this invention is a mature grass carp IL-21 peptide. The pET-28a-IL-21 recombinant expression plasmid was constructed by optimizing the IL-21 base codons, transformed into an *E. coli* (DE3) expression system, and soluble grass carp IL-21 recombinant protein was obtained by IPTG induction. Furthermore, this invention also found that the optimized gene, its recombinant protein, and plasmids can enhance the intestinal barrier integrity of grass carp, inhibit the load of pathogenic bacteria in the intestine, and repair intestinal damage caused by bacterial infection. This indicates that the grass carp IL-21 recombinant protein has a significant effect on protecting intestinal homeostasis, highlighting its promising application prospects in the research and development of prevention and treatment of bacterial enteritis in aquatic animals.

The bacillus subtilis strain against aeromonas and its application, the bacillus subtilis strain of the present application is named bacillus subtilis (wgb0621), and the preservation number is CCTCC NO: M 2026135. Bacillus subtilis The bacillus subtilis strain has good contact inhibition activity on aeromonas veronii and aeromonas hydrophila, can improve the survival rate of grass carp after being infected with aeromonas, has good safety, and is not dependent on diffusible bacteriostatic substances. The bacillus subtilis strain is suitable for biological prevention and control of aeromonas disease in aquaculture, and has good application prospect.

This invention belongs to the field of aquaculture biotechnology and discloses the application of a grass carp FGF1 recombinant protein in the preparation of products against Aeromonas hydrophila infection. The amino acid sequence of the recombinant protein is shown in SEQ ID NO:2. This invention is the first to discover that this known protein effectively reduces the pathogen load in tissues such as the liver and spleen by repairing the intestinal mucosal physical barrier (significantly increasing the number of intestinal goblet cells, promoting epithelial cell proliferation, and inhibiting apoptosis) and strengthening the immune barrier (upregulating key antibacterial factors such as IL-22, MUC2, Spla2, and HB-EGFa), and significantly increases the 14-day survival rate of grass carp infected with Aeromonas hydrophila from 10% to 56.67%. This invention overcomes the shortcomings of existing technologies that rely solely on in vitro sterilization or are limited to non-infectious factors, providing a green and efficient biological product solution based on host barrier repair for bacterial diseases in grass carp.

The application of FGF8 protein in the preparation of antibacterial agents is provided. Escherichia coli BL21 (DE3) is used to express grass carp FGF8a and murine FGF8b. After purification through Ni2+-TED agarose gel column, recombinant proteins of grass carp FGF8a and murine FGF8b are obtained. Both proteins exhibit significant bactericidal effects against Gram-negative bacteria and Gram-positive bacteria, and can be used to prepare broad-spectrum antibacterial agents or antibacterial drugs, demonstrating high application value.

The application discloses a SNP molecular marker related to grass carp weight on a 19th chromosome of the grass carp and an application thereof, and belongs to the technical field of biology. The nucleotide sequence of the SNP molecular marker is shown in SEQ ID NO. 1, and the polymorphism of two allelomorphic genes A and G at the 501st base. The application further provides an amplification primer of the SNP molecular marker, and the nucleotide sequences of the amplification primer are shown in SEQ ID NO. 2 and SEQ ID NO. 3, which are used for grass carp genotyping or breeding, and the method comprises the following steps: detecting DNA of a to-be-tested grass carp by using the SNP molecular marker and the amplification primer, selecting the grass carp with the genotype AG for breeding, and the average weight of the grass carp with the genotype AG is large. The application introduces molecular marker assisted selection in the process of breeding the grass carp, overcomes the problems of long breeding cycle, low efficiency and large environmental influence of the traditional breeding method, and realizes early screening and directional breeding of excellent traits of the grass carp.

The application belongs to the field of aquatic organism breeding, and particularly relates to a SNP site of grass carp TLR5 related to grass carp hemorrhagic disease resistance, a SNP site combination and application thereof. The application successfully screens the SNP site of grass carp TLR5 related to grass carp hemorrhagic disease resistance through PCR-RFLP sequencing and correlation analysis, which is located at the 30969284th and 30958638th base of chromosome 20. In view of the two SNP sites, a SNP site combination of TLR5 related to grass carp hemorrhagic disease resistance is further developed, and the grass carp hemorrhagic disease resistance individual can be screened through detection of the SNP site combination. Therefore, the SNP molecular marker provided by the application can be used for grass carp molecular marker assisted breeding, and accelerate the breeding of disease-resistant grass carp varieties, and provide a reference basis for the disease-resistant breeding of grass carp.