104 results about "Periplaneta" patented technology

An extract of American periplaneta and its medicinal composition with high content of general amino acids are disclosed. Its preparing process includes such steps as immersing American periplaneta in petroether for removing fat, extracting in water or alcohol, and mixing with pharmacologically acceptable additives.

The medicinal American giant roach extractum preparing process includes alcohol extraction process and water extraction process. The alcohol extraction process includes hot water killing of roach, drying, crushing, sieving; alcohol soaking for 40-50 hr; alcohol reflux extraction in a multifunctional extractor; recovering alcohol; recovering medicine liquid via oil-water separation; and filtering and concentrating medicine liquid to obtain extractum. The water extraction process includes extraction of the material after recovering alcohol in alcohol extraction process in distilled water; recovering medicine liquid and oil-water separation; filtering and concentrating medicinal liquid; and cooling to obtain extractum. The said preparing process has high yield, and the product has total amino acid content up to 50.4 %.

The invention discloses new dihydroiso-coumarin glucoside compounds shown in a formula I, which are extracted and separated from Periplaneta americanna medicinal materials. Through mouse auricle swelling tests, rat granulation tissue hyperblastosis tests and the like, results show that the compounds have effects of resisting inflammation, promoting wounds to heal and the like; and the compounds can be used for preparing medicines for treating inflammation, ulcers, wounds, incised wounds, fistula, burn, scalding and the like, particularly medicines for preventing and treating diseases related to inflammation resistance, promotion of granulation tissue hyperblastosis, wound healing and the like, and have high clinical application value. The invention provides a preparation method for the compounds shown in the formula I.

The present invention relates to Periplaneta Americana or its ethanol extract for the treatment of inflammation. Specifically, said inflammation includes cervical erosion, ulcerative colitis, skin ulcer, mucosal ulcer and postoperative condyloma acuminatum. Treating said disorders by Periplaneta Americana or its ethanol extract has the advantages of effectively reducing bleeding, shortering healing time, significant curative effect, no adverse reactions and low cost. Periplaneta Americana or its ethanol extract provides a new selection for the clinical treatment of cervical erosion, ulcerative colitis and postoperative condyloma acuminatum.

The invention relates to the field of medical technology, and especially relates to a method for preparing a phthisic adjuvant with extractum of Periplaneta Americana. The invention provides the method for preparing a phthisic adjuvant with extractum of Periplaneta Americana, wherein the method comprises the following steps of: A, smashing; B, soaking; C, extracting; D, first concentration; E, degreasing; and F, second concentration. With the method, a lot of proteins in the Periplaneta americana can be removed effectively, the grease removal rate is high, and the oil content of the obtained extract is only 0.30 counted by A(absorbance) / W(Weight). By the alcohol with concentration of 90%-95%, the adjuvant can be prevented from spoilage, the preservation time is prolonged, and the yield of active ingredients is improved. The content of the active ingredients is up to 13%-22%, the cure rate of the prepared medicine to the phthisis is up to 45%, and the total effective rate can be 85%. Hydrolysis of active substances (in the extract) for adjunctive treatment to the phthisis is prevented, and the therapeutic effect of the adjuvant is improved. The production cost is lowered, and the production efficiency is improved.

The invention relates to the feed processing field, and especially refers to a special feed for Periplaneta americana adults and a preparation method of the same. The special feed for Periplaneta americana adults is composed of a mixture of the following constituents in parts by weight: 40-50 parts of corn, 25-35 parts of wheat bran, 12-16 parts of bean pulp, 1 part of shells of incubated Periplaneta americana nymphs and egg shells, 6-8 parts of fish meal, 0.2-0.3 part of table salt, 0.1-0.2 part of choline chloride, 0.1-0.2 part of copper sulfate, 0.1-0.2 part of potassium iodide and 5-6 parts of oil. Moreover, 10-20 parts of rice bran, 2-4 parts of brown sugar, 30-45 parts of beet and 6-8 parts of sugar residue also can be added. The preparation method comprises the following steps of: A, mixing main materials in parts by weight, then, adding water, stirring and mixing evenly, and stacking for 2-5 days at normal temperature; and B, adding additives in the product of the step A in parts by weight. The feed is relatively low in cost, the Periplaneta americana grows quickly, and the internal amino acid content of the Periplaneta americana is high, so that the Periplaneta americana is high in phamaceutical value.

The invention provides a method for extracting medicinal substances from Periplaneta American Linn, which comprises the following steps: A, alcohol extraction: killing and soaking larvae in alcohol; freezing the larvae at a low temperature; cracking shells, carrying out coarse-sizing, placing in an extractor and soaking in alcohol; thermal-reflux alcohol-extracting, recovering the drug liquid, placing in a separator and carrying out the oil-water separation; filtering and concentrating the separated drug liquid; discharging and cooling the drug liquid to obtain the medicinal substances; and B, water extraction: pouring the materials of alcohol extraction into distilled water; carrying out heating extraction; recovering and placing the drug liquid in the separator and carrying out the oil-water separation; and filtering and concentrating to obtain the medicinal substances. Compared with the prior art, the method of the invention improves the extraction yield and increases the total content of amino acid as well as the contents of polypeptide, protein, chitin, chitosan and the like in the extracted medicinal substances, and is particularly characterized in that the unpleasant odor is eliminated by low-temperature freezing before the extraction, therefore, the extracted medicinal substances have almost no unpleasant odor from the original extract while more bioactive substances are preserved.

The invention discloses periplaneta americana extract and its preparation method and use. The preparation method comprises the following steps of carrying out acetone backflow, water extraction and condensation, adding ethanol into the concentrate, carrying out ethanol recovery to obtain the filtrate without ethanol, treating the filtrate without ethanol by an adsorption resin column, collecting the eluant until eluant ninhydrin reaction is negative, by a polyamide column, collecting the eluant until eluant ninhydrin reaction is negative, and carrying out condensation and drying. The invention also discloses the novel use of the periplaneta americana extract. The preparation method overcomes the defects of the traditional preparation method and has a high application value.

The invention discloses dsRNA designed based on an Orco gene of periplaneta americana, and a coding gene, a preparation method and an application thereof. The dsRNA is a double-stranded RNA composed of a nucleotide sequence shown in SEQ ID NO.1 and used as a positive-sense strand and a nucleotide sequence reversely complementary to the nucleotide sequence shown in SEQ ID NO.1 and used as an antisense strand. The dsRNA can be widely applied in preparation of products for preventing and treating the periplaneta americana, interfering the chemical communication of the periplaneta americana or controlling the mating and reproduction of adult periplaneta americana, or preventing and controlling the periplaneta americana and other insects having the same gene target sequence. By blocking key genes of an olfactory system of periplaneta americana, the induction effect of sex pheromone to male insects is inhibited, mating and reproduction of the periplaneta americana can be effectively controlled, and a new technology and strategy are provided for prevention and control of the periplaneta americana.

The present invention relates to Periplaneta Americana or its ethanol extract for the treatment of inflammation. Specifically, said inflammation includes cervical erosion, ulcerative colitis, skin ulcer, mucosal ulcer and postoperative condyloma acuminatum. Treating said disorders by Periplaneta Americana or its ethanol extract has the advantages of effectively reducing bleeding, shortering healing time, significant curative effect, no adverse reactions and low cost. Periplaneta Americana or its ethanol extract provides a new selection for the clinical treatment of cervical erosion, ulcerative colitis and postoperative condyloma acuminatum.

The invention discloses an artificial propagation method for periplaneta americana. The method comprises the following steps: a sack, an incubator, a breeder box and an egg tray are sealed and fumigated for one hour after being evenly mixed with disinfecting solution; the temperature in the incubator is controlled between 29 DEG C and 33 DEG C through an automatic temperature and humidity control device, the relative humidity is controlled between 80 percent and 85 percent, and the inside of the incubator is well ventilated; a layer of sack is arranged at the bottom part of the breeder box or a hatching bamboo sieve, a layer of ootheca with a thickness of 1 cm to 3 cm is placed on the sack, a layer of egg tray is transversely placed on the ootheca, and a layer of sack is also covered on the egg tray; the breeder box or the hatching bamboo sieve is placed on a partition board with a hole in the incubator or on a hatchery rack of a hatching house; and when 10 to 30 larvae are generated in each egg tray, the egg trays in which the larvae are hidden are gently transferred to breeding chips, and the larvae hidden in the egg trays naturally enter in the chips to grow. The method can overcome the problems of low hatchability and long hatching period, and is suitable for the large-scale artificial propagation of the periplaneta americana.

An orally taken target medicine for treating the ulcerative colitis with high curative effect and low dosage is prepared from the extract of American periplaneta. Its preparing process is also disclosed.

The invention provides a preparation method for a degreased periplaneta americana linnaeus extract. The preparation method comprises the following steps: (1) killing and grinding; (2) degreasing; (3)extracting; (4) concentration under reduce pressure; (5) degreasing and filtering; (6) macroporous adsorption resin column chromatography; (7) concentration under reduce pressure. According to the degreased periplaneta americana linnaeus extract prepared by the preparation method, ineffective components such as grease, polysaccharide and macromolecular protein are effectively removed, polypeptidecomponents with good anti-ulcer activity in periplaneta americana Linnaeus are enriched, the content of the polypeptide component reaches 85% or more; and the obtained degreased periplaneta americanalinnaeus extract has light color and is odor-free.

The invention relates to a periplaneta americana medicinal material fingerprint quality determination method. The fingerprint obtained by the periplaneta americana medicinal material fingerprint quality determination method has a good chromatographic peak resolution degree. The determination method has reasonable detection time and good repeatability and stability. Compared with the existing reported periplaneta americana medicinal material fingerprint technology, the method improves a periplaneta americana medicinal material quality control level. The fingerprint technology effectively evaluates and controls periplaneta americana medicinal material quality, guarantees periplaneta americana extract quality stability under a certain preparation condition, further effectively controls quality of periplaneta americana extract-containing preparations such as Kangfuxin tablets, Kangfuxin capsules, a Kangfuxin liquid, Xinmailong, Ganlong capsules and the like.

The invention discloses a quality control method of Periplaneta americana freeze-dried powder. The quality control method comprises the steps of carrying out amino acid identification by virtue of a thin layer chromatography, carrying out content determination on amino acid and polypeptide by virtue of ultraviolet-visible spectrophotometry, and carrying out content determination on uracil, hypoxanthine and creatinine by virtue of a high performance liquid chromatography. According to the quality control method, the quality of the Periplaneta americana freeze-dried powder is detected and controlled from several aspects of the qualitative identification of amino acid, the content determination of amino acid, polypeptide and nucleoside components, the composition analysis of amino acid and the molecular weight determination of polypeptide. Powerful guarantee is provided for the quality of the Periplaneta americana freeze-dried powder, and the quality and the treatment effect of a later-period preparation taking the Periplaneta americana freeze-dried powder as an intermediate is guaranteed.

The invention relates to the technical field of medicine, in particular to a method for extracting and processing periplaneta americana. The method for extracting and processing periplaneta americanacomprises the following steps that A, smashing is carried out; B, ethanol soaking is carried out; C, reflux extraction is carried out; D, decompression concentration is carried out; E, grease layeringis carried out; F, decompression concentration is carried out to obtain periplaneta americana extract extractum; G, the periplaneta americana extract extractum, sugar and water are taken by weight, stirred and mixed uniformly and filtered; H, high temperature sterilization is carried out; I, lactic acid bacteria inoculation and anaerobic fermentation are carried out; J, post-ripeness is carried out; K, vacuum freeze drying is carried out, and then smashing is carried out to obtain a periplaneta americana extraction and fermentation product. The method for extracting and processing periplanetaamericana has the advantages that active ingredients in the periplaneta americana are gathered, macromolecular substances are reduced, and the content of micromolecular active substances is increased, so that the peculiar smell of insects of the periplaneta americana extraction and fermentation product is relieved or eliminated.

The invention relates to a facial mask containing periplaneta americana cosmetic ingredients and a preparing method thereof. The facial mask is made from water and functional cosmetic substances, wherein the functional cosmetic substances comprise a preservative, essence, a periplaneta americana raw material and a functional raw material, and the periplaneta americana raw material is any one of concentrated pulp prepared from periplaneta americana living bodies, periplaneta americana dried powder, periplaneta americana extract liquid, periplaneta americana extract cream and periplaneta americana extract powder; the functional raw material is any one of a whitening efficacy raw material, a moistening efficacy raw material, a freckle lightening efficacy raw material, an anti-wrinkle efficacy raw material, an anti-allergic efficacy raw material, an oil-control efficacy raw material, a cleansing efficacy raw material and an acne removing efficacy raw material. The facial mask can perform a whitening, moistening, freckle lightening, anti-wrinkle, anti-allergic, oil-control, cleansing or acne removing function. The facial mask can clear blocked pores, clean grease and promote the skin to absorb nutrients so as to realize cosmetic effect, and has natural health-care functions.

The invention discloses a medicine composite for treating cervical erosion and a preparation method thereof. The medicine composite mainly comprises a mixture of active ingredients prepared from periplaneta Americana extract, pomegranate peel and citric acid, and the active ingredients are in the following proportion by weight: 100 parts of periplaneta Americana extract, 1-180 parts of pomegranate peel and 1-200 parts of citric acid. The preparation method of the medicine composite for treating cervical erosion comprises the following steps of: extracting the periplaneta Americana extract, extracting active ingredients of the pomegranate peel, mixing the extracted ingredients, and then adding in citric acid for preparing the medicine composite. In the invention, the medicine composite has simple preparation method, gives full play to the function of the periplaneta Americana extract, effectively improves the curative effect on the cervical erosion, is convenient to use, has tiny toxic and side effects, and is a new medicament worthy of generalization.

The invention discloses a pharmaceutical composition for treating condyloma acuminatum and herpes, mainly comprising mixtures of effective components of 100 parts of periplaneta Americana extractive and 2-300 parts of granatum. The method for preparing the pharmaceutical composition for treating condyloma acuminatum and herpes comprises the following steps of: extracting the periplaneta Americana extractive and the effective components of the granatum, and mixing to prepare the medicine. The preparation method of the invention is simple, gives full play to the periplaneta Americana extractive, and effectively improves the healing effect of condyloma acuminatum and herpes; the pharmaceutical composition is a new medicine worth of promoting, is convenient to use and has little toxic and side-effect.

The invention discloses a hedgehog signal pathway gene associated with amputated limb regeneration of Periplaneta americana and application of dsRNA thereof. The results showed that Hh gene, Ptc gene,Smo gene and / or Ci gene had the ability to affect the limb regeneration of Periplaneta americana. Moreover, silencing the expression of Hh, Ptc, Smo and Ci genes could inhibit the regeneration ability of Periplaneta americana, which could not only remove the pests of Periplaneta americana efficiently, but also avoid the environmental pollution and is safe and reliable. Hh, Ptc, Smo and Ci gene ofthat invention can be used as control target of Periplaneta americana pest control.

The invention discloses a method for preparing D-glucosamine hydrochloride by taking periplaneta americana as raw material. The method comprises the steps of extracting chitin from the periplaneta Americana, and preparing the D-glucosamine hydrochloride from the prepared chitin. According to the method disclosed by the invention, the chitin is extracted from the periplaneta americana by adopting an extraction method through an organic solvent under unique process conditions, thus the extraction rate is high, the quality of the extracted chitin is excellent, and the medical-grade D-glucosamine hydrochloride with the excellent quality can be further prepared; and by adopting the method, an extract from the periplaneta americana and extraction remains can be effectively utilized, and the utilization rate of the raw material can be greatly improved.

The invention relates to the field of species identification and provides a kit to identify Periplaneta americana. The kit includes a positive control, forward and reverse primers, a PCR (polymerase chain reaction) mixed liquid and distilled water. The positive control is DNA barcode standard detection fragment COII shown as SEQ ID NO. 1; the forward and reverse primers have sequences shown as inSEQ ID NO. 2 and SEQ ID NO. 3. In the invention, genetic fragment COII (SEQ ID NO. 1) from Periplaneta americana has homology of 98-100% in the species of Periplaneta americana, and has significantlyreduced gene homology against non-Periplaneta americana species; primer design and PCR amplification help accurately distinguish Periplaneta americana and other similar species. The kit provided herein is suitable for identifying powdery or processed Periplaneta americana, and has a wider detection range, higher detection accuracy and short identifying time.

The invention provides a periplaneta americana extract and a preparation process thereof. The preparation process comprises the following steps: soaking periplaneta americana bodies in 60-79% ethanol, conducting reflux extraction, concentration and degreasing. The preparation process disclosed by the invention is simple to operate and is capable of saving cost and energy. The periplaneta americana extract prepared by the method disclosed by the invention has the following advantages: the periplaneta americana extract is high in content of active ingredients, wherein the content of free amino acid can achieve 3.54g / 100g of crude drug or above; and the periplaneta americana extract is obvious in therapeutic effect on UC, free from fishy smell and light in color.

The invention discloses a periplaneta Americana medicine composition for treating pharyngitis and tonsillitis and a preparation method thereof. The preparation method includes the steps that firstly, effective components are mixed, weighing is performed according to the proportion of extracts of Periplaneta Americana, to honeysuckle extracts and to radix scutellariae extracts is (0.8-1.2): (0.5-5.0): (1.0-10.0), then the extracts are evenly mixed, a mixture of the effective components is prepared and waits for use; secondly, the prepared medicine is obtained, the mixture of the effective components is prepared into the periplaneta Americana medicine composition for treating pharyngitis and tonsillitis. The preparation method is simple and feasible, the functions of all the medicine are given full play to, and the treatment effect on acute or chronic pharyngitis, acute or chronic tonsillitis and upper respiratory tract infection is remarkably improved.

The invention discloses a periplaneta Americana pharmaceutical composition for treating ulcerative colitis, mainly comprising mixtures of effective components prepared from the following components in parts by weight: 0.8-1.2 parts of periplaneta Americana extract, 0.1-2.5 parts of coptis, 0.15-4.5 parts of scutellaria baicalensis, 0.15-6 parts of golden cypress and 0.15-15 parts of rheum officinale. The method for preparing the periplaneta Americana pharmaceutical composition for treating ulcerative colitis comprises the following steps of: preparing the periplaneta Americana extract, weighing, boiling and mixing the effective components to prepare the medicine composition. The preparation method is simple, gives full play to the periplaneta Americana extract and effectively improves the curative effect of the ulcerative colitis.

The invention provides a composition for improving skin quality. The composition is prepared from the following medicinal preparations in parts by weight: 4-16 parts of periplaneta Americana extract and 8-24 parts of purslane extract. The invention further provides application of the composition. The composition has an excellent effect of treating eczema, can be used for effectively inhibiting moisture loss of eczema skins, relieving inflammatory reactions and reducing skin ulceration, inflammation and other pathological injuries, and has good application prospect.

The invention relates to the field of feed processing, and especially relates to a special feed for removing metal and lipid in Periplaneta americana adults and a preparation method of the same. The special feed for removing metal and lipid in Periplaneta americana adults is composed of a mixture of the following constituents in parts by weight: 5-15 parts of corns, 3-10 parts of wild carrot, 5-15 parts of rice bran, 1 part of pig blood and 0.005-0.01 part of table salt, wherein 0.05-0.1 part of brown sugar and 3-5 parts of sugar residue also can be added in the feed, 0.5-1 part of tea leaves and 0.5-1 part of agaric also can be added in the feed, and 10-15 parts of beet also can be added in the feed. The preparation method comprises the following steps of: mixing raw materials in part by weight, then, adding water, stirring and mixing evenly, covering the mixture with a thermal insulation material, and stacking for 2-5 days at normal temperature. By feeding the adults with the feed for 3-10 days before killing the adults, the internal lipid and heavy metal of the Periplaneta americana adults can be removed effectively, thus, the following medicine processing cost is reduced, and the medicine safety is higher.

The invention relates to a multi-PCR primer and a method for quickly identifying a blattaria insect variety, belonging to the technical field of applied biology. The multi-PCR primer for quickly identifying the blattaria insect variety comprise a periplaneta americana primer, a periplaneta fuliginosa primer, a periplaneta australasiae primer, a periplaneta brunnea primer, a blattella germanica primer and a ground beetle primer. The identifying method comprises the following steps: extracting blattaria insect genome total DNA, performing multi-PCR amplification, and judging a variety of a blattaria insect to be detected according to PCR product size obtained by sepharose gel electrophoresis detection. PCR product strip sizes of periplaneta americana, periplaneta fuliginosa, periplaneta australasiae, periplaneta brunnea, blattella germanica and ground beetle are respectively 452bp, 125bp, 160bp, 284bp, 253bp and 380bp. The identifying primer and the multi-PCR method can be used for sensitively and quickly determining the variety of the blattaria insect sample to be detected, and the method has the advantages of simple operation, high repeatability, low cost and the like in comparisonto a traditional detection method.

The invention relates to an external-use burn analgesic ointment for treating burned patients, relating to the technical field of traditional Chinese medicines and aiming to solving the problem of unideal treatment effect in the prior art. In order to solve the problem in the prior art, the invention has the technical scheme that the external-use burn analgesic ointment comprises the following components in parts by weight: 15 parts of angelica sinensis, 11 parts of cacumen biotae, 10 parts of radix arnebiae seu lithospermi, 880 parts of sesame oil, 100 parts of rhubarbs, 100 parts of periplaneta, 50 parts of cape jasmine fruits, 30 parts of borneols, 30 parts of peach kernels and 40 parts of camphor. Compared with the prior art, the invention has the advantages of good treatment effect, quick effect, outstanding curative effect, extensive indication, suitability for the treatment of various burns and scalds, safety, reliability, simple preparation process and easy industrialized production realization without toxicity or nocuity or any toxic and side effect on human bodies.