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65 results about "Nymph" patented technology

In biology, a nymph is the immature form of some invertebrates, particularly insects, which undergoes gradual metamorphosis (hemimetabolism) before reaching its adult stage. Unlike a typical larva, a nymph's overall form already resembles that of the adult, except for a lack of wings (in winged species). In addition, while a nymph moults it never enters a pupal stage. Instead, the final moult results in an adult insect. Nymphs undergo multiple stages of development called instars.

Laodelphax striatellus ecdysis gene LsMH and application thereof in biological control of Laodelphax striatellus

ActiveCN119391715Aharm reductionAchieve biological controlBiocideHormone peptidesBiotechnologyNymph
According to the laodelphax striatellus ecdysis gene LsMH and the application thereof in biological prevention and control of laodelphax striatellus, the laodelphax striatellus ecdysis gene LsMH serves as one of key genes for ecdysis of laodelphax striatellus, the laodelphax striatellus ecdysis gene LsMH can be subjected to targeted interference, laodelphax striatellus can be remarkably inhibited from forming adults through eclosion, and laodelphax striatellus death is avoided, so that laodelphax striatellus population is the harm of the laodelphax striatellus to food crops is reduced. The control of laodelphax striatellus is carried out through a biological means, the method is safe and harmless to environment ecology and food, and a new strategy is provided for green control of laodelphax striatellus. The dsRNA or the interference reagent is prepared through transcription of the laodelphax striatellus ecdysis gene LsMH, the LsMH gene can be effectively interfered after the dsRNA or the interference reagent is injected into the laodelphax striatellus, the phenomena that wings are bent or incomplete, the abdomen is wizened and ecdysis is incomplete occur in the ecdysis process of laodelphax striatellus nymphs, laodelphax striatellus eclosion into adults is inhibited, laodelphax striatellus death is avoided, and biological control over laodelphax striatellus is achieved.
Owner:PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI

Method for mass rearing of cyrtopimpla spp.

The present application relates to a kind of methods for mass propagation of tea corner miridae in laboratory, comprising the following technical steps: collecting tea garden tea corner miridae adult, cultivating fall kui seedling, putting seedling and leading insect, tea corner miridae habitat and feeding, mating and oviposition and nymph hatching and feeding.The present application uses fall kui seedling as the main body of feeding raw material, and the leaf of raw material is relatively thick, strong in harm resistance, not easy to dry, which helps to improve the hatching rate of tea corner miridae eggs, reduce the frequency of raw material supply and replacement, at the same time, the present application saves time and effort, can be realized in laboratory low cost and sustainability fast, efficient, mass propagation of tea corner miridae test insect source.
Owner:INST OF PLANT PROTECTION HAINAN ACAD OF AGRI SCI (HAINAN ACAD OF AGRI SCI AGRI PROD QUALITY SAFETY & STANDARDS RES CENT)

Hyperspectral and morphological feature fused rice planthopper stage and sex identification method

The invention discloses a hyperspectral and morphological feature fused rice planthopper stage and sex identification method, and aims to improve the classification accuracy of the variety, age and sex of rice planthoppers in the initial stage of nymphs. Spectral data of rice planthoppers are obtained through a hyperspectral imaging technology, morphological characteristics are extracted by combining a morphological image processing technology, and the spectral data and morphological characteristic data are combined by adopting a multi-modal data fusion technology. And training and classifying the fused data by using a machine learning algorithm, and accurately identifying the type, age and gender of the rice planthopper. In order to solve the problem of data imbalance, an SMOTE algorithm is adopted to carry out sample oversampling, and class distribution balance is ensured. The severity of insect pests is evaluated in real time through automatic counting and population density generation, and support is provided for prevention and control measures. The screening of characteristic wave bands is expected to provide key technical support for the development of special multispectral cameras. In addition, the model interpretability is improved by adopting SHAP analysis, and the contribution of the features to the classification result is quantified.
Owner:SICHUAN AGRI UNIV

Breeding method of cantheconidea furcellata

The invention provides a breeding method of cantheconidea furcellata, and belongs to the technical field of insect feeding. The invention provides a breeding method of cantheconelaconus furcellatus. The breeding method comprises the step of feeding the cantheconelaconus furcellatus with frozen larvae of spodoptera litura. According to the method, fresh lapidoptera furcellata larvae are used for feeding lapidoptera furcellata, second-year-old lapidoptera furcellata nymphs can only eat second-year-old and lower-year-old lapidoptera furcellata larvae, and in the age, the lapidoptera furcellata nymphs do not die in a large number. According to the method, the survival rate of the low-age nymphs of the cantheconidea furcellatus can be remarkably increased by feeding the cantheconidea furcellatus with the frozen cantheconidea furcellatus. Meanwhile, the frozen older podoptera litura larvae are used for feeding the cantheconidea furcellata, so that the use amount of the cantheconidea furcellata is greatly reduced, mass feeding is facilitated, the labor efficiency is improved, the labor cost is saved, and large-scale feeding is facilitated. The breeding method is suitable for large-scale breeding of the cantheconidea furcellatus, so that manual rapid large-scale breeding and long-term subculture breeding of the cantheconidea furcellatus are realized, and a new way is opened up for controlling noctuidae pests which are difficult to control.
Owner:INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI +1

Radish aphid sirt1 gene and application and method for preventing and treating radish aphid

The present application relates to the field of insect control, and particularly relates to a SIRT1 gene of Rhopalosiphum padi and application thereof and a method for controlling Rhopalosiphum padi. The gene encodes a protein with an amino acid sequence as shown in SEQ ID NO: 2. The present application finds that the SIRT1 gene plays a role in the growth and development of Rhopalosiphum padi. Inhibition of the expression of SIRT1 can significantly increase the mortality rate of Rhopalosiphum padi nymphs, significantly reduce the proportion of winged aphids, and ultimately cause the population to decline and the toxin transmission ability to weaken. By using RNA interference technology, it is found that inhibition of the expression of the SIRT1 gene of Rhopalosiphum padi can significantly increase the mortality rate of the nymphs, and significantly reduce the proportion of winged adults after development. In addition, by spraying dsRNA of the SIRT1 gene interference sequence of Rhopalosiphum padi encapsulated by a nano material, the mortality rate of the Rhopalosiphum padi nymphs is significantly increased.
Owner:HUAZHONG AGRI UNIV

PCR (Polymerase Chain Reaction) primer combination for detecting three types of thrips in cotton field and multiple PCR detection method

The invention provides a PCR (Polymerase Chain Reaction) primer combination and a multiplex PCR detection method for detecting three types of thrips in a cotton field, and relates to a one-step multiplex PCR molecular rapid identification primer and method for three types of common thrips (tobacco thrips, flower thrips and western flower thrips) in the cotton field based on a mitochondrial COI gene. Specific upstream primers are respectively designed for three types of thrips and are combined with universal downstream primers, a one-step multiplex PCR detection system is constructed by optimizing the primer proportion and annealing temperature, a single target strip is amplified by a single template through single-tube reaction for one time, and specific strips of 123 bp (tobacco thrips), 417 bp (flower thrips) and 322 bp (flower thrips) can be synchronously amplified in a mixed sample template. And accurate identification of the three thrips is realized. According to the method, the limitation of traditional morphological identification in nymph stage and sibling species distinguishing is broken through, the detection efficiency is improved by 3 times compared with that of single PCR, and efficient technical support is provided for dynamic monitoring and green prevention and control of cotton field thrip populations.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES

Bemisia tabaci nymph development gene COX8 and application thereof

The invention relates to a bemisia tabaci gene BtCOX8 and application thereof, and belongs to the field of biological control. The gene BtCOX8 highly expressed in the bemisia tabaci is screened, dsRNA is synthesized for the gene, the dsRNA is dripped on the body surface of nymphs of the bemisia tabaci, the nymphs are obviously shrunken and wizened, are in an obvious dehydration state and show the characteristics of growth resistance and serious physiological function disorder, and the results show that the BtCOX8 is a potential efficient RNAi lethal target. The dsRNA synthesized by the invention can be used for biological control of bemisia tabaci, is safe to encarsia formosa and orius minutus which are natural enemies of the bemisia tabaci, has the advantages of environmental friendliness and the like, and has a good application prospect.
Owner:GUIZHOU UNIV

Isoxazoline compound containing benzamide as well as preparation method and application of isoxazoline compound

The invention belongs to the technical field of organic matter preparation, and provides an isoxazoline compound containing benzamide as well as a preparation method and application thereof.Chemical modification and molecular design are performed on a compound with an isoxazoline structure, a substituent group is introduced to N of an amide group, and the benzamide-containing isoxazoline compound is prepared. A series of high-efficiency compounds which can be used for killing insects or mites in agriculture or forestry and have excellent activity are obtained, and particularly, the compounds have very good biological activity on pests of lepidoptera, hemiptera and the like. When being used at an extremely low concentration, the compound disclosed by the invention also shows an excellent control effect on various pests or pest mites, has insecticidal or acaricidal activity in each stage (such as eggs, larvae (nymphs), pupae and adults) of the life cycle of the pests or pest mites, and has an excellent control effect on the pests or pest mites which have drug resistance to traditional insecticides or acaricides. And the composition also shows excellent control activity.
Owner:THE KEY LAB OF CHEM FOR NATURAL PROD OF GUIZHOU PROVINCE & CHINESE ACADEMY OF SCI

A thrips control technology system based on bacteria and thrips complementation for honey pomelo fruiting stage

ActiveCN118525699BPlant protectionAnimal husbandryFrankliniella bispinosaNymph
The application discloses a thrips biological control technology system based on bacteria and thrips complementation, which is characterized in that, in the pre-flowering stage, the ground and underground soil are used as the main body, the thrips pupae are controlled as the target, and the bacteria and soil-dwelling predatory mites are used as complementary objects; in the flowering and fruiting stage, the honey pomelo flowers and fruits on the ground are used as the main body, the adults and nymphs of the scirtothrips citri and flower thrips are controlled as the target, and the bacteria and thrips are used as complementary objects; in the post-fruiting stage, the honey pomelo branches and leaves on the ground are used as the main body, the scirtothrips citri and scirtothrips citri are controlled as the target, and the leaf-dwelling predatory mites carrying bacteria are used as the main agent. The application firstly utilizes the cheese mite to prey on the thrips pupae once, then utilizes the soil-dwelling predatory mites to prey on the thrips pupae twice, and finally utilizes the reproduction process of the thrips to prey on the thrips larvae on the ground three times; meanwhile, the cheese mite is also the food of the southern flower bug or the ladybug, forms an integrated biological chain, introduces the thrips into the bottom end of the biological chain, and in addition, the bacteria are toxic to the thrips, so that the generation of the thrips is almost eliminated, and the thrips control effect in the flowering and fruiting stage of the honey pomelo is greatly improved.
Owner:INST OF PLANT PROTECTION FAAS

Artificial efficient breeding method for golden cicadas

The artificial efficient breeding method comprises the following steps that S1, host plants are planted in a spawning room, and the host plants are gramineae plants or asparagus plants which are easy to plant and have developed root systems; s2, collecting mature golden cicada nymphs which come out of the ground and are not subjected to eclosion, putting the collected mature golden cicada nymphs into an artificial climate box for artificial eclosion, and after eclosion and bee emergence, selecting parent adults; s3, putting the adults into a mating cage of an oviposition room according to a male-female ratio for mating, wherein an adult luring device consisting of a male cicada chirp generating device and a sex pheromone trap is arranged in the oviposition room. By arranging the adult luring device composed of the male cicada chirp generating device and the sex pheromone trap, the mating rate of the male cicada and the female cicada is synergistically increased, and technical support is provided for subsequently increasing the breeding rate of a cicada provenance; by means of the breeding method, efficient artificial breeding of the golden cicada seed source is achieved, and a seed source resource is provided for large-scale breeding of the golden cicada.
Owner:JIANGSU ACAD OF AGRI SCI

Indoor efficient propagation method for tadelcoris suturalis based on snap beans

The invention relates to the field of artificial feeding of insects, and particularly provides an indoor efficient propagation method for tadelcoris lucorum based on snap beans. According to the method, fresh and mature snap bean pods are used as feeding and spawning hosts of adult teardiocoris suturalis, and feeding is conducted under the indoor conditions that the temperature is 28 DEG C, the humidity is 80% and the photoperiod is 12 L: 12 D; the vigna unguiculata containing eggs is taken out for moisturizing culture, and after incubation, tender vigna unguiculata is supplemented in time for nymphs to eat. According to the method, by optimizing host selection and environmental conditions, the three-day egg laying amount of the female tapolygus lucorum on the snap beans reaches 129 or above, the egg hatching rate reaches 71.3%, and the survival rate of nymphs reaches 80% or above. The method is convenient in material taking, low in cost and simple to operate, can effectively solve the problems of difficulty in indoor breeding, low egg laying rate, low hatching rate and the like of the adelphocoris suturalis in the prior art, and is suitable for standard and large-scale breeding of an adelphocoris suturalis test insect source in a laboratory.
Owner:SANYA RESEARCH INSTITUTE OF HAINAN ACADEMY OF AGRICULTURAL SCIENCES (HAINAN EXPERIMENTAL ANIMAL RESEARCH CENTER) +1

A spore-producing strain of entomogenous fungus and its application in the prevention and treatment of western flower thrips

ActiveCN121555330BBiocideFungiBiotechnologyFrankliniella bispinosa
The present application relates to the field of microbial technology, and discloses a spore-producing Metarhizium anisopliae strain and its application in the prevention and treatment of western flower thrips. The Metarhizium anisopliae provided by the present application has a preservation number of CCTCC No. M 20252118, a fast growth rate, a large spore yield, a high spore germination rate, and is easy to be cultured and multiplied in large quantities indoors. The present application can be used as a biocontrol fungus for preventing and treating the invasive agricultural pest western flower thrips, has strong pathogenicity to the western flower thrips nymphs, is safe to the environment, is not easy to make the pests resistant, and has application value.
Owner:HUNAN AGRI UNIV

Method for increasing hatching rate of eggs of military mating miridae and collecting newly hatched nymphs

The invention provides a method for increasing the hatching rate of eggs of military mating miridae and collecting newly hatched nymphs, and belongs to the field of biological control. The invention discloses a method for improving the hatching rate of eggs of military mating miridae and collecting newly hatched nymphs, in the hatching of the eggs of military mating miridae, main veins and side veins of platanus acerifolia leaves producing the eggs of military mating miridae are cut and placed in a culture dish, and the humidity in the culture dish is controlled, so that the hatching rate of the eggs of military mating miridae is remarkably improved and reaches up to 90%; transferring of newly hatched nymphs is completed by adding fresh platanus leaves into a culture dish; the collection of the newly hatched nymphs of the military mating miridae is completed by directly putting fresh platanus leaves with the newly hatched nymphs into an insect breeding box, so that the survival rate of the nymphs reaches 100%. The method is easy and convenient to operate, the situation that platanus acerifolia leaves are easily dried up or mildewed due to poor humidity control is avoided, and the hatching rate of the military mating miridae eggs is effectively increased; meanwhile, the situation that newly-hatched nymphs are transferred through a writing brush in a traditional method is avoided, and the survival rate of the newly-hatched nymphs in
Owner:SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING

Bemisia tabaci nymph development gene BtPri39 and application thereof

The invention relates to a bemisia tabaci nymph development gene BtPri39 and application thereof, and belongs to the field of biological control. The gene BtPri39 highly expressed in the bemisia tabaci is screened, dsRNA is synthesized for the gene, the dsRNA is dripped on the body surface of nymphs of the bemisia tabaci, the nymphs are obviously shrunken and wizened, are in an obvious dehydration state and show the characteristics of growth resistance and serious physiological function disorder, and the results show that the BtPri39 is an efficient RNAi lethal target with potential. The dsRNA synthesized by the invention can be used for biological control of bemisia tabaci, is safe to encarsia formosa which is the natural enemy of the bemisia tabaci, has the advantages of environmental friendliness and the like, and has a very good application prospect.
Owner:GUIZHOU UNIV

Processing and preparation technology of cicada tea

A kind of tea processing preparation process, it is related to tea processing technical field, solve the problem of different water content of tea fresh leaves due to different degree of sucking and biting of small green leafhopper adults and nymphs and different time interval from sucking and biting to picking.The preparation process includes fresh leaf pretreatment, collision green and aroma enhancement, steam + high hot air fixation, spreading, striping, drying, screening and picking, aroma enhancement and packaging.In the fresh leaf pretreatment process, small green leafhopper adults are put in, and small green leafhoppers are attracted to suck and bite fresh leaves by yellow light.In the collision green process, tea fresh leaves are made to jump by vibration to carry out collision green treatment.The present application solves the problem of different degree of fresh leaf water loss due to different degree of sucking and biting of small green leafhopper adults and nymphs and different time interval from sucking and biting to picking by putting small green leafhopper adults and nymphs to concentrate sucking and biting of tea fresh leaves.Meanwhile, the quality of cicada tea is effectively improved by temperature and humidity control, light supplement, collision green and aroma enhancement, steam + high hot air fixation.
Owner:QIANSHAN COUNTY JINMA ECOLOGICAL AGRI DEV CO LTD +1

High-toxicity metarhizium anisopliae strain CIPPMA362 resistant to high temperature and ultraviolet stress and application thereof

The invention relates to the field of insect prevention and control, in particular to a high-toxicity metarhizium anisopliae strain IPPMA362 resistant to high temperature and ultraviolet stress and application thereof. The invention aims to screen excellent metarhizium anisopliae strains with high toxicity to bemisia tabaci. Through indoor biological activity determination, the lethal ability of the target strain on the bemisia tabaci nymphs is determined; further, key biological characteristics such as sporulation capability, spore germination rate and ultraviolet resistance of the strain are systematically evaluated, so that the application potential of the strain is judged; and the optimized strain is accurately identified through a molecular biology means, so that a foundation is laid for subsequent strain registration, patent protection and product development.
Owner:INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI +1

Method for breeding periplaneta americana

The invention belongs to the technical field of breeding, and provides a breeding method of periplaneta americana. The breeding method of periplaneta americana comprises the following steps: (1) feeding young nymphs: feeding newly hatched nymphs in a breeding box for 30-40 days by adopting first feed to obtain young nymphs; the first feed comprises the following components in parts by weight: 30-36 parts of basic grains, 4-5 parts of composite acid-base materials and 1.2-1.8 parts of composite Chinese herbal medicines; (2) feeding the young nymphs: continuously feeding the young nymphs in the breeding box for 3-5 months by adopting second feed until adult nymphs are obtained; the second feed comprises the following components in parts by weight: 30-36 parts of basic grains, 2-3 parts of composite acid-base materials and 4-6 parts of composite Chinese herbal medicines. According to the breeding method, the periplaneta americana nymphs are bred under different conditions in stages, and a new feed formula is adopted, so that the requirements of the periplaneta americana nymphs in different growth stages are met, the digestion and absorption functions of the periplaneta americana nymphs in different growth stages are improved, the breeding cycle is shortened, and the survival rate, breeding efficiency and product medicinal quality of periplaneta americana are improved.
Owner:SICHUAN GOODDOCTOR PANXI PHARMA

A method for artificial breeding of scutellerus tenuipes

PendingCN122271279ANymphMating
This invention relates to the field of artificial breeding technology for natural enemy insects, and in particular to a method for the artificial breeding of the assassin bug *Triplophysa pulcherrima*. The invention employs steps including seed source collection and screening, rearing environment control, feed supply and management, mating and reproduction management, oviposition and egg mass collection, nymph hatching and age-differentiated rearing, overwintering management, and cannibalism control. By providing specialized rearing management devices, temperature and humidity conditions, standardized feed feeding, age-differentiated rearing density control, and moisture-controlled egg mass hatching, it solves the problems of high mortality rates among young nymphs, unstable mating success rates, low egg mass hatching rates, and the lack of a year-round breeding system in existing technologies. The method of this invention is simple to operate, has a high survival rate (nymphal survival rate exceeding 85%), an egg mass hatching rate exceeding 90%, and can achieve 4-5 generations per year, enabling large-scale, year-round production of *Triplophysa pulcherrima*, providing high-quality natural enemy resources for the biological control of agricultural and forestry pests.
Owner:INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY

Tetranychus urticae classification identification and automatic counting system and method based on deep learning

The invention discloses a tetranychus urticae classification identification and automatic counting system and method based on deep learning, and relates to the technical field of agricultural pest monitoring. By integrating the optical microscope, the high-precision image acquisition equipment and the deep learning model, automatic detection and counting functions of mites are realized, and the technical problems of low efficiency, high subjectivity, poor accuracy and the like in a traditional manual detection method are solved; by adopting an improved YOLO series algorithm and a multi-scale feature fusion technology, the system can identify multiple development stages (including eggs, larvae, nymphs and adult mites) and predatory mites of tetranychus urticae at the same time, and the detection accuracy and efficiency are greatly improved.
Owner:CHINA TOWER CO LTD

LsDHX16 gene of laodelphax striatellus coding RNA helicase and application of LsDHX16 gene in biological control

The invention discloses an LsDHX16 gene of laodelphax striatellus coding RNA helicase and application of the LsDHX16 gene in biological control, and belongs to the technical field of biology. After the expression level of the gene is knocked down in the laodelphax striatellus by using an RNA interference technology, the external genital organ of the female laodelphax striatellus is deformed and the female laodelphax striatellus dies due to ecdysis failure, so that the sex proportion of the laodelphax striatellus is influenced; meanwhile, the development of the amphoteric nymphs is stagnated. The abnormal phenomena show that the LsDHX16 gene plays an important role in sex differentiation and growth processes of laodelphax striatellus. In view of high specificity of the gene participating in regulation and control of insect sex determination, the gene can be used as a target spot for accurately preventing and controlling laodelphax striatellus, and dsRNA targeting the gene can achieve the purpose of effectively preventing and controlling laodelphax striatellus by disturbing the sex proportion of laodelphax striatellus and reducing the population number of laodelphax striatellus. In conclusion, the invention provides a theoretical basis for biological control of laodelphax striatellus.
Owner:NINGBO UNIV +1

A method and system for raising and harvesting orthoptera

System for orthoptera, such as crickets, with an enclosure 1 defining a cavity 4, a habitat structure 8 with a plurality of vertically extending channels 10, a collection region and container 14 for frass directly beneath the channels, and access means, such as a closable opening 16, to remove the frass or orthoptera from the structure. The frass or orthoptera may be removed while the habitat structure remains in situ. There may be a flexible climbing means 18, such as string or rope, to allow crickets to climb up into the channels. There may be a region above the habitat for holding food. There may be a lid or heater. 21 The habitat structure may be carboard sheets 9 or egg cartons. A method of raising and harvesting crickets is also disclosed, which comprises locating eggs or nymphs in the enclosure, killing or incapacitating the orthoptera when they are ready to be harvested, and removing the orthoptera which have fallen into the collecting region from the enclosure. The orthoptera may be killed by heating.
Owner:BALDERSON DANIEL

Azobenzene 4-trifluoromethyl nicotinamide derivative as well as preparation method and application thereof

PendingCN121591650ABiocideSilicon organic compoundsNymphArthropod mouthparts
The invention relates to an azobenzene 4-trifluoromethyl nicotinamide derivative as well as a preparation method and application thereof, in particular to the azobenzene 4-trifluoromethyl nicotinamide derivative with a structure as shown in a formula I. The azobenzene 4-trifluoromethyl nicotinamide derivative has excellent insecticidal activity. The pesticide can effectively prevent and treat insects with piercing-sucking mouthparts such as alfalfa aphids, and especially has a very good prevention and treatment effect on one-day-old nymphs of alfalfa aphids.
Owner:EAST CHINA UNIV OF SCI & TECH

Cyctria megacephala odorant binding protein and application thereof

According to the odorant binding protein for the black-tailed leafhopper and the application of the odorant binding protein, by interfering the expression quantity of the binding protein in the black-tailed leafhopper, the perceptual ability of the black-tailed leafhopper to volatile matters induced by rice dwarf viruses can be effectively inhibited, so that the effect of preventing and treating the rice dwarf viruses is achieved. The amino acid sequence of the odorant binding protein is SEQ ID NO: 1. According to the application disclosed by the invention, a fluorescent competitive binding experiment finds that the odorant binding protein NcinOBP7 of the black tail leafhopper and a volatile matter released by rice induced by a rice dwarf virus have high affinity; an RNAi experiment shows that the rice volatile matter induced by the rice dwarf virus has an attraction effect on the black-tail leafhopper nymphs injected with dsEGFP, but after the NcinOBP7 is silenced, the rice volatile matter does not attract the black-tail leafhopper nymphs any more. Therefore, the silence of the black-tail leafhopper NcinOBP7 can influence the perception of the black-tail leafhopper on the rice volatile matters, and subsequently speculates to inhibit the feeding of the black-tail leafhopper on the virus-infected rice and influence the transmission of viruses.
Owner:QINGDAO AGRI UNIV

Oviposition collecting device suitable for large-scale breeding of cantheconidea furcellata

ActiveCN222621972UReduce difficultyReduce the frequency of artificial hydrationAnimal husbandryNymphAdult worm
The utility model discloses an oviposition collecting device suitable for large-scale breeding of cantheconidea furcellata, which comprises a nymph breeding net cage, a connecting pipe, an adult breeding net cage, an oviposition suspension line, an oviposition frame, a feed basin and oviposition cloth, one end of the connecting pipe is communicated with the nymph breeding net cage, and the other end of the connecting pipe is communicated with the adult breeding net cage; the oviposition suspension line is arranged at the top of the adult feeding net cage, and the oviposition frame and the feed basin are arranged in the adult feeding net cage; and the spawning cloth is suspended on the spawning suspension line and / or the spawning frame. The device has the advantages that the difficulty of manual pairing and manual egg taking can be greatly reduced, the labor cost in the breeding process is remarkably reduced, meanwhile, the frequency of manual water replenishing is reduced, the breeding efficiency and the egg taking efficiency are remarkably improved, and the device is very suitable for large-scale breeding and egg taking of cantheconidea furcellata. The resource guarantee is provided for further improving the biological control effect and efficiency.
Owner:ZHIXIN BIOLOGICAL (GUANGZHOU) CO LTD

Brown planthopper G-protein coupled receptor Methuselah-like 2 and application thereof

The invention discloses a brown planthopper G-protein coupled receptor Methuselah-like 2 and application of the brown planthopper G-protein coupled receptor Methuselah-like 2. The amino acid sequence of the G-protein coupled receptor Methuselah-like 2 is as shown in SEQ ID NO. 2. The dsRNA (dsMthl2) of the brown planthopper G-protein coupled receptor Methuselah-like 2 disclosed by the invention has better gene silencing efficiency, so that the ecdysis rate of brown planthopper nymphs can be obviously reduced, epidermis dysplasia can be caused, an ordered and uniform endodermis structure cannot be formed, the outer epidermis is thickened, and the structure is loose. The brown planthopper G-protein coupled receptor Methuselah-like 2 disclosed by the invention can be used as an important molecular target for preventing and controlling brown planthopper, is expected to fully play the function of ecological prevention and control while inhibiting pests, and can be widely applied to crop breeding, biopesticide research and development and biological prevention and control.
Owner:YANGZHOU UNIV

Sex pheromone of eurygaster integriceps puton and preparation method and application thereof

PendingCN122325330ANymphPesticide residue
This invention belongs to the field of pest control technology, and provides a sex pheromone for the tea mirid bug, its preparation method, and its application. The sex pheromones of the tea mirid bug of this invention are (R)-3-butoxybutyrate octyl ester, (S)-3-butoxybutyrate octyl ester, and (R)-3-butoxybutyrate hexyl ester. The sex pheromones of the tea mirid bug of this invention are non-toxic, do not need to be directly applied to tea trees, pose no pesticide residue risk to tea leaves, and are harmless to beneficial insects in tea gardens. They are highly effective in attracting and killing male tea mirid bugs, directly killing large numbers of male tea mirid bugs that damage tea leaves, disrupting the male-to-female ratio, reducing mating success rates, and indirectly controlling the next generation of nymphs from damaging tea trees. The slow-release lure made from the sex pheromone of this invention contains milligrams of the tea mirid bug's sex pheromone, making it highly efficient, and its effective period lasts up to 32 days. Long-term use can maintain long-term suppression of the male tea mirid bug population.
Owner:TEA RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES

Lipaphis erysimi SIRT1 gene, application thereof and method for preventing and treating Lipaphis erysimi

The invention relates to the field of insect prevention and control, in particular to a Lipaphis erysimi SIRT1 gene, application thereof and a Lipaphis erysimi prevention and control method. The gene encodes a protein with an amino acid sequence as shown in SEQ ID NO: 2. The effect of the SIRT1 gene in the lipaphis erysimi growth and development process is found, the death rate of lipaphis erysimi nymphs can be remarkably increased by inhibiting expression of the SIRT1, the proportion of winged aphids is remarkably reduced, and finally population development declines, and the virus transmission capacity is weakened. The RNA interference technology is used to find that the expression of the Lipaphis erysimi SIRT1 gene is inhibited, the nymph death rate of the Lipaphis erysimi is obviously increased, and the winged type ratio of the Lipaphis erysimi developed into imagoes is obviously reduced. In addition, dsRNA of a lipaphis erysimi SIRT1 gene interference sequence packaged by a nano material is sprayed, so that the death rate of lipaphis erysimi nymphs is remarkably increased.
Owner:HUAZHONG AGRI UNIV

Selatheconelaconus furcellatus attractant as well as preparation method and application thereof

The invention discloses a cantheconidea furcellata attractant as well as a preparation method and application thereof, and belongs to the technical field of plant protection. Comprising 5-8 parts of cyclohexanone, 3-5 parts of fucus extract and 1-3 parts of n-eicosane. According to the cantheconidea furcellata attractant and preparation and use of the cantheconidea furcellata attractant, pests can be effectively controlled, negative effects on the environment can be reduced, natural enemy populations can be protected, and the cantheconidea furcellata attractant is a more sustainable and environment-friendly pest management strategy. Experiments prove that the attractant prepared in the embodiment not only can effectively improve the colonization rate of adult cantheconidea furcellata in tobacco plants, but also can improve the colonization rate of five-year-old nymphs of cantheconidea furcellata in tobacco plants. The method is an ideal means for pest control.
Owner:YUNNAN AGRICULTURAL UNIVERSITY

Attractant for cantheconidea furcellata

The invention discloses an attractant for cantheconidea furcellata, and belongs to the technical field of plant protection. Comprising the following components in parts by weight: 3-5 parts of tetradecanal, 2-3 parts of fucus extract, 1-2 parts of n-eicosane and 1-2 parts of trans-2-hexenal. The invention discloses an attractant for cantheconidea furcellata, the cantheconidea furcellata attractant attracts nymphs and adults of the cantheconidea furcellata by simulating chemical signals in nature, the predation ability of the cantheconidea furcellata to pests is enhanced, meanwhile, the use of chemical pesticides is reduced, and the cantheconidea furcellata attractant is environment-friendly and is an effective biological prevention and control means. Experiments prove that the attractant prepared by the embodiment of the invention can effectively improve the colonization rate of cantheconidea furcellata in tobacco plants, the colonization rate of cantheconidea furcellata adults is as high as 78.7%, and the cantheconidea furcellata attractant can be used as a cantheconidea furcellata colonization attractant in tobacco fields, and is an ideal means for pest control.
Owner:YUNNAN AGRICULTURAL UNIVERSITY