35 results about "Nymph" patented technology

The present application relates to a kind of methods for mass propagation of tea corner miridae in laboratory, comprising the following technical steps: collecting tea garden tea corner miridae adult, cultivating fall kui seedling, putting seedling and leading insect, tea corner miridae habitat and feeding, mating and oviposition and nymph hatching and feeding.The present application uses fall kui seedling as the main body of feeding raw material, and the leaf of raw material is relatively thick, strong in harm resistance, not easy to dry, which helps to improve the hatching rate of tea corner miridae eggs, reduce the frequency of raw material supply and replacement, at the same time, the present application saves time and effort, can be realized in laboratory low cost and sustainability fast, efficient, mass propagation of tea corner miridae test insect source.

The present application relates to the field of insect control, and particularly relates to a SIRT1 gene of Rhopalosiphum padi and application thereof and a method for controlling Rhopalosiphum padi. The gene encodes a protein with an amino acid sequence as shown in SEQ ID NO: 2. The present application finds that the SIRT1 gene plays a role in the growth and development of Rhopalosiphum padi. Inhibition of the expression of SIRT1 can significantly increase the mortality rate of Rhopalosiphum padi nymphs, significantly reduce the proportion of winged aphids, and ultimately cause the population to decline and the toxin transmission ability to weaken. By using RNA interference technology, it is found that inhibition of the expression of the SIRT1 gene of Rhopalosiphum padi can significantly increase the mortality rate of the nymphs, and significantly reduce the proportion of winged adults after development. In addition, by spraying dsRNA of the SIRT1 gene interference sequence of Rhopalosiphum padi encapsulated by a nano material, the mortality rate of the Rhopalosiphum padi nymphs is significantly increased.

The invention provides a PCR (Polymerase Chain Reaction) primer combination and a multiplex PCR detection method for detecting three types of thrips in a cotton field, and relates to a one-step multiplex PCR molecular rapid identification primer and method for three types of common thrips (tobacco thrips, flower thrips and western flower thrips) in the cotton field based on a mitochondrial COI gene. Specific upstream primers are respectively designed for three types of thrips and are combined with universal downstream primers, a one-step multiplex PCR detection system is constructed by optimizing the primer proportion and annealing temperature, a single target strip is amplified by a single template through single-tube reaction for one time, and specific strips of 123 bp (tobacco thrips), 417 bp (flower thrips) and 322 bp (flower thrips) can be synchronously amplified in a mixed sample template. And accurate identification of the three thrips is realized. According to the method, the limitation of traditional morphological identification in nymph stage and sibling species distinguishing is broken through, the detection efficiency is improved by 3 times compared with that of single PCR, and efficient technical support is provided for dynamic monitoring and green prevention and control of cotton field thrip populations.

The invention relates to a bemisia tabaci gene BtCOX8 and application thereof, and belongs to the field of biological control. The gene BtCOX8 highly expressed in the bemisia tabaci is screened, dsRNA is synthesized for the gene, the dsRNA is dripped on the body surface of nymphs of the bemisia tabaci, the nymphs are obviously shrunken and wizened, are in an obvious dehydration state and show the characteristics of growth resistance and serious physiological function disorder, and the results show that the BtCOX8 is a potential efficient RNAi lethal target. The dsRNA synthesized by the invention can be used for biological control of bemisia tabaci, is safe to encarsia formosa and orius minutus which are natural enemies of the bemisia tabaci, has the advantages of environmental friendliness and the like, and has a good application prospect.

The invention belongs to the technical field of organic matter preparation, and provides an isoxazoline compound containing benzamide as well as a preparation method and application thereof.Chemical modification and molecular design are performed on a compound with an isoxazoline structure, a substituent group is introduced to N of an amide group, and the benzamide-containing isoxazoline compound is prepared. A series of high-efficiency compounds which can be used for killing insects or mites in agriculture or forestry and have excellent activity are obtained, and particularly, the compounds have very good biological activity on pests of lepidoptera, hemiptera and the like. When being used at an extremely low concentration, the compound disclosed by the invention also shows an excellent control effect on various pests or pest mites, has insecticidal or acaricidal activity in each stage (such as eggs, larvae (nymphs), pupae and adults) of the life cycle of the pests or pest mites, and has an excellent control effect on the pests or pest mites which have drug resistance to traditional insecticides or acaricides. And the composition also shows excellent control activity.

The application discloses a thrips biological control technology system based on bacteria and thrips complementation, which is characterized in that, in the pre-flowering stage, the ground and underground soil are used as the main body, the thrips pupae are controlled as the target, and the bacteria and soil-dwelling predatory mites are used as complementary objects; in the flowering and fruiting stage, the honey pomelo flowers and fruits on the ground are used as the main body, the adults and nymphs of the scirtothrips citri and flower thrips are controlled as the target, and the bacteria and thrips are used as complementary objects; in the post-fruiting stage, the honey pomelo branches and leaves on the ground are used as the main body, the scirtothrips citri and scirtothrips citri are controlled as the target, and the leaf-dwelling predatory mites carrying bacteria are used as the main agent. The application firstly utilizes the cheese mite to prey on the thrips pupae once, then utilizes the soil-dwelling predatory mites to prey on the thrips pupae twice, and finally utilizes the reproduction process of the thrips to prey on the thrips larvae on the ground three times; meanwhile, the cheese mite is also the food of the southern flower bug or the ladybug, forms an integrated biological chain, introduces the thrips into the bottom end of the biological chain, and in addition, the bacteria are toxic to the thrips, so that the generation of the thrips is almost eliminated, and the thrips control effect in the flowering and fruiting stage of the honey pomelo is greatly improved.

The artificial efficient breeding method comprises the following steps that S1, host plants are planted in a spawning room, and the host plants are gramineae plants or asparagus plants which are easy to plant and have developed root systems; s2, collecting mature golden cicada nymphs which come out of the ground and are not subjected to eclosion, putting the collected mature golden cicada nymphs into an artificial climate box for artificial eclosion, and after eclosion and bee emergence, selecting parent adults; s3, putting the adults into a mating cage of an oviposition room according to a male-female ratio for mating, wherein an adult luring device consisting of a male cicada chirp generating device and a sex pheromone trap is arranged in the oviposition room. By arranging the adult luring device composed of the male cicada chirp generating device and the sex pheromone trap, the mating rate of the male cicada and the female cicada is synergistically increased, and technical support is provided for subsequently increasing the breeding rate of a cicada provenance; by means of the breeding method, efficient artificial breeding of the golden cicada seed source is achieved, and a seed source resource is provided for large-scale breeding of the golden cicada.

The invention relates to the field of artificial feeding of insects, and particularly provides an indoor efficient propagation method for tadelcoris lucorum based on snap beans. According to the method, fresh and mature snap bean pods are used as feeding and spawning hosts of adult teardiocoris suturalis, and feeding is conducted under the indoor conditions that the temperature is 28 DEG C, the humidity is 80% and the photoperiod is 12 L: 12 D; the vigna unguiculata containing eggs is taken out for moisturizing culture, and after incubation, tender vigna unguiculata is supplemented in time for nymphs to eat. According to the method, by optimizing host selection and environmental conditions, the three-day egg laying amount of the female tapolygus lucorum on the snap beans reaches 129 or above, the egg hatching rate reaches 71.3%, and the survival rate of nymphs reaches 80% or above. The method is convenient in material taking, low in cost and simple to operate, can effectively solve the problems of difficulty in indoor breeding, low egg laying rate, low hatching rate and the like of the adelphocoris suturalis in the prior art, and is suitable for standard and large-scale breeding of an adelphocoris suturalis test insect source in a laboratory.

The present application relates to the field of microbial technology, and discloses a spore-producing Metarhizium anisopliae strain and its application in the prevention and treatment of western flower thrips. The Metarhizium anisopliae provided by the present application has a preservation number of CCTCC No. M 20252118, a fast growth rate, a large spore yield, a high spore germination rate, and is easy to be cultured and multiplied in large quantities indoors. The present application can be used as a biocontrol fungus for preventing and treating the invasive agricultural pest western flower thrips, has strong pathogenicity to the western flower thrips nymphs, is safe to the environment, is not easy to make the pests resistant, and has application value.

The invention provides a method for increasing the hatching rate of eggs of military mating miridae and collecting newly hatched nymphs, and belongs to the field of biological control. The invention discloses a method for improving the hatching rate of eggs of military mating miridae and collecting newly hatched nymphs, in the hatching of the eggs of military mating miridae, main veins and side veins of platanus acerifolia leaves producing the eggs of military mating miridae are cut and placed in a culture dish, and the humidity in the culture dish is controlled, so that the hatching rate of the eggs of military mating miridae is remarkably improved and reaches up to 90%; transferring of newly hatched nymphs is completed by adding fresh platanus leaves into a culture dish; the collection of the newly hatched nymphs of the military mating miridae is completed by directly putting fresh platanus leaves with the newly hatched nymphs into an insect breeding box, so that the survival rate of the nymphs reaches 100%. The method is easy and convenient to operate, the situation that platanus acerifolia leaves are easily dried up or mildewed due to poor humidity control is avoided, and the hatching rate of the military mating miridae eggs is effectively increased; meanwhile, the situation that newly-hatched nymphs are transferred through a writing brush in a traditional method is avoided, and the survival rate of the newly-hatched nymphs in

The invention relates to a bemisia tabaci nymph development gene BtPri39 and application thereof, and belongs to the field of biological control. The gene BtPri39 highly expressed in the bemisia tabaci is screened, dsRNA is synthesized for the gene, the dsRNA is dripped on the body surface of nymphs of the bemisia tabaci, the nymphs are obviously shrunken and wizened, are in an obvious dehydration state and show the characteristics of growth resistance and serious physiological function disorder, and the results show that the BtPri39 is an efficient RNAi lethal target with potential. The dsRNA synthesized by the invention can be used for biological control of bemisia tabaci, is safe to encarsia formosa which is the natural enemy of the bemisia tabaci, has the advantages of environmental friendliness and the like, and has a very good application prospect.

A kind of tea processing preparation process, it is related to tea processing technical field, solve the problem of different water content of tea fresh leaves due to different degree of sucking and biting of small green leafhopper adults and nymphs and different time interval from sucking and biting to picking.The preparation process includes fresh leaf pretreatment, collision green and aroma enhancement, steam + high hot air fixation, spreading, striping, drying, screening and picking, aroma enhancement and packaging.In the fresh leaf pretreatment process, small green leafhopper adults are put in, and small green leafhoppers are attracted to suck and bite fresh leaves by yellow light.In the collision green process, tea fresh leaves are made to jump by vibration to carry out collision green treatment.The present application solves the problem of different degree of fresh leaf water loss due to different degree of sucking and biting of small green leafhopper adults and nymphs and different time interval from sucking and biting to picking by putting small green leafhopper adults and nymphs to concentrate sucking and biting of tea fresh leaves.Meanwhile, the quality of cicada tea is effectively improved by temperature and humidity control, light supplement, collision green and aroma enhancement, steam + high hot air fixation.

The invention relates to the field of insect prevention and control, in particular to a high-toxicity metarhizium anisopliae strain IPPMA362 resistant to high temperature and ultraviolet stress and application thereof. The invention aims to screen excellent metarhizium anisopliae strains with high toxicity to bemisia tabaci. Through indoor biological activity determination, the lethal ability of the target strain on the bemisia tabaci nymphs is determined; further, key biological characteristics such as sporulation capability, spore germination rate and ultraviolet resistance of the strain are systematically evaluated, so that the application potential of the strain is judged; and the optimized strain is accurately identified through a molecular biology means, so that a foundation is laid for subsequent strain registration, patent protection and product development.

This invention relates to the field of artificial breeding technology for natural enemy insects, and in particular to a method for the artificial breeding of the assassin bug *Triplophysa pulcherrima*. The invention employs steps including seed source collection and screening, rearing environment control, feed supply and management, mating and reproduction management, oviposition and egg mass collection, nymph hatching and age-differentiated rearing, overwintering management, and cannibalism control. By providing specialized rearing management devices, temperature and humidity conditions, standardized feed feeding, age-differentiated rearing density control, and moisture-controlled egg mass hatching, it solves the problems of high mortality rates among young nymphs, unstable mating success rates, low egg mass hatching rates, and the lack of a year-round breeding system in existing technologies. The method of this invention is simple to operate, has a high survival rate (nymphal survival rate exceeding 85%), an egg mass hatching rate exceeding 90%, and can achieve 4-5 generations per year, enabling large-scale, year-round production of *Triplophysa pulcherrima*, providing high-quality natural enemy resources for the biological control of agricultural and forestry pests.

The invention discloses a tetranychus urticae classification identification and automatic counting system and method based on deep learning, and relates to the technical field of agricultural pest monitoring. By integrating the optical microscope, the high-precision image acquisition equipment and the deep learning model, automatic detection and counting functions of mites are realized, and the technical problems of low efficiency, high subjectivity, poor accuracy and the like in a traditional manual detection method are solved; by adopting an improved YOLO series algorithm and a multi-scale feature fusion technology, the system can identify multiple development stages (including eggs, larvae, nymphs and adult mites) and predatory mites of tetranychus urticae at the same time, and the detection accuracy and efficiency are greatly improved.

The invention relates to an azobenzene 4-trifluoromethyl nicotinamide derivative as well as a preparation method and application thereof, in particular to the azobenzene 4-trifluoromethyl nicotinamide derivative with a structure as shown in a formula I. The azobenzene 4-trifluoromethyl nicotinamide derivative has excellent insecticidal activity. The pesticide can effectively prevent and treat insects with piercing-sucking mouthparts such as alfalfa aphids, and especially has a very good prevention and treatment effect on one-day-old nymphs of alfalfa aphids.

According to the odorant binding protein for the black-tailed leafhopper and the application of the odorant binding protein, by interfering the expression quantity of the binding protein in the black-tailed leafhopper, the perceptual ability of the black-tailed leafhopper to volatile matters induced by rice dwarf viruses can be effectively inhibited, so that the effect of preventing and treating the rice dwarf viruses is achieved. The amino acid sequence of the odorant binding protein is SEQ ID NO: 1. According to the application disclosed by the invention, a fluorescent competitive binding experiment finds that the odorant binding protein NcinOBP7 of the black tail leafhopper and a volatile matter released by rice induced by a rice dwarf virus have high affinity; an RNAi experiment shows that the rice volatile matter induced by the rice dwarf virus has an attraction effect on the black-tail leafhopper nymphs injected with dsEGFP, but after the NcinOBP7 is silenced, the rice volatile matter does not attract the black-tail leafhopper nymphs any more. Therefore, the silence of the black-tail leafhopper NcinOBP7 can influence the perception of the black-tail leafhopper on the rice volatile matters, and subsequently speculates to inhibit the feeding of the black-tail leafhopper on the virus-infected rice and influence the transmission of viruses.

The invention discloses a brown planthopper G-protein coupled receptor Methuselah-like 2 and application of the brown planthopper G-protein coupled receptor Methuselah-like 2. The amino acid sequence of the G-protein coupled receptor Methuselah-like 2 is as shown in SEQ ID NO. 2. The dsRNA (dsMthl2) of the brown planthopper G-protein coupled receptor Methuselah-like 2 disclosed by the invention has better gene silencing efficiency, so that the ecdysis rate of brown planthopper nymphs can be obviously reduced, epidermis dysplasia can be caused, an ordered and uniform endodermis structure cannot be formed, the outer epidermis is thickened, and the structure is loose. The brown planthopper G-protein coupled receptor Methuselah-like 2 disclosed by the invention can be used as an important molecular target for preventing and controlling brown planthopper, is expected to fully play the function of ecological prevention and control while inhibiting pests, and can be widely applied to crop breeding, biopesticide research and development and biological prevention and control.

This invention belongs to the field of pest control technology, and provides a sex pheromone for the tea mirid bug, its preparation method, and its application. The sex pheromones of the tea mirid bug of this invention are (R)-3-butoxybutyrate octyl ester, (S)-3-butoxybutyrate octyl ester, and (R)-3-butoxybutyrate hexyl ester. The sex pheromones of the tea mirid bug of this invention are non-toxic, do not need to be directly applied to tea trees, pose no pesticide residue risk to tea leaves, and are harmless to beneficial insects in tea gardens. They are highly effective in attracting and killing male tea mirid bugs, directly killing large numbers of male tea mirid bugs that damage tea leaves, disrupting the male-to-female ratio, reducing mating success rates, and indirectly controlling the next generation of nymphs from damaging tea trees. The slow-release lure made from the sex pheromone of this invention contains milligrams of the tea mirid bug's sex pheromone, making it highly efficient, and its effective period lasts up to 32 days. Long-term use can maintain long-term suppression of the male tea mirid bug population.

The application discloses a breeding method of Harpactor fuscipes and application thereof in prevention and control of mulberry pyralid, and belongs to the technical field of pest control. The application comprises the following steps: (1) capturing adult Harpactor fuscipes from the wild, feeding, oviposition, and then hatching and breeding the eggs; (2) 1st instar nymphs: from hatching to the first molting, a mixed solution is used to dip a cotton ball to obtain a mixed solution cotton ball, the mixed solution cotton ball is placed in a hatching box, and the hatched Harpactor fuscipes feeds by itself; (3) after 2nd instar: in addition to the mixed solution cotton ball in the step (2), the 2nd instar to 4th instar Harpactor fuscipes is fed with artificial feed of corresponding instar silkworm, and the 5th instar Harpactor fuscipes and adult Harpactor fuscipes is fed with 4th instar artificial feed of silkworm. The method has high breeding rate and low cost, and the bred Harpactor fuscipes has remarkable effect in application in prevention and control of mulberry pyralid.

The invention provides a cordyceps sobolifera strain 022017-9 and a biocontrol agent and application thereof, and belongs to the technical field of functional microorganisms. According to the invention, one strain is separated from aphid bodies which are dead due to diseases, the number of the strain is 022017-9, and the strain is determined as Cordyceps cicadae through morphological characteristic observation and a molecular biological identification technology. The strain 022017-9 has a good prevention and control effect on the adult stage and / or the nymph stage of aphids. The strain 022017-9 can obviously reduce the survival rate of aphids and the egg laying amount, and meanwhile, the net reproduction rate Ro, the average generation duration T, the intrinsic growth rate rm, the weeklimit growth rate lambda and other parameters of an inoculated aphid population are all obviously changed. Therefore, the strain 022017-9 has relatively strong pathogenicity to the aphids and has a good application prospect in the aspect of biological control of the aphids.

This invention belongs to the field of insect rearing technology, specifically relating to a method for artificially rearing the entire life cycle of the spotted bug (Aegilops spp.), comprising the following steps: S1, placing spotted bug eggs in a culture dish and transferring them to an intelligent artificial climate chamber until the nymphs hatch; S2, transferring the spotted bug nymphs from the intelligent artificial climate chamber to a rearing cage, and feeding them a full-life-cycle feed daily; S3, after the adults emerge, pairing the male and female adults, and feeding them a full-life-cycle feed daily, transferring the eggs to a rearing tube after the female lays eggs. The full-life-cycle feed for spotted bugs is composed of the following components by mass fraction: 90%-95% legume feed and 5%-10% egg yolk protein-loaded enzyme preparation. This method avoids contamination from spotted bug excrement and other impurities, reduces the probability of mutation and mortality, and improves the efficiency of nutrient absorption by spotted bugs.

The invention discloses a biological assay method for evaluating the influence of gene editing wheat on aphids, and belongs to the technical field of safety evaluation of gene editing crops. The bioassay method comprises the following steps: selecting a single wheat seedling after wheat seedlings emerge, wrapping the stem base of the single wheat seedling with wet absorbent cotton, fixing the stem base in a tube, then inoculating single-head wing-free adult aphids on the wheat seedling, removing the adult aphids and redundant young aphids after the adult aphids produce young aphids for the first time, and only retaining one young aphid. And from the beginning of the first-stage aphid production, observing once under a stereoscopic microscope every day, recording the growth and development conditions of the aphids, and removing aphid slough and dead individuals in time. And evaluating the influence of the gene editing wheat on the aphids by taking the survival rate of the aphids on the tenth day, the early aphid production stage, the life and the aphid production quantity as evaluation indexes. The method is wide in applicability and can be effectively used for evaluating the influence of various kinds of gene editing wheat on various aphids such as schizaphis graminum, sitobion avenae and rhopalosiphum padi.

The invention discloses a two-stage artificial breeding method for Harpactor fuscipes, and belongs to the technical field of biological control of agricultural pests, and the method comprises the following steps: breeding bean aphids by planting broad beans, and breeding newly hatched Harpactor fuscipes by using the bean aphids; then mulberry leaves are used for breeding silkworms, 1-3-year-old silkworm larvae are used for breeding 2-5-year-old sacharius fuscipes nymphs, the sacharius fuscipes nymphs and adult sacharius fuscipes are used for hunting lepidoptera pests in an orchard, and harm of the lepidoptera pests to fruit trees is reduced. The method utilizes low-value materials, is low in cost and simple to operate, can effectively reduce the population density of lepidoptera pests in the orchard and reduce fruit loss of the orchard, and has a wide application prospect in the field of orchard pest control.

The invention discloses a periplaneta americana breeding method which comprises the following steps: step 1, preparing equipment, using a modular breeding box, dividing the interior of the modular breeding box into four independent breeding areas, namely a larva area, a nymph area, an adult area and a spawning area, separating the areas through sealing partition plates, and arranging independent environment regulation and control modules, the environment regulation and control module comprises a temperature control module, a humidity control module and an illumination regulation and ventilation system; 2, seed insect screening: selecting American cockroach adults with no body surface damage, quick activity, no deformity and excrement formation, the weight of a single American cockroach adults being greater than or equal to 1g, the male-female ratio being 3: 1 and the putting density being 100-200 adults per square meter, disinfecting a culture box before putting, performing spray disinfection on the culture box by adopting a 0.5% peracetic acid aqueous solution before putting, and ventilating for 24 hours after disinfection, after no disinfectant is left in the box body, seed insects are put in. The invention relates to the technical field of breeding, and particularly provides a breeding method of periplaneta americana.

The invention provides an ecological seed bag suitable for nymph alpine wetland and application of the ecological seed bag, and belongs to the technical field of ecological modification, and the seed bag is prepared from the following seeds according to mass: 1.5 kg / mu of elymus nutans, 0.5 kg / mu of tall cattail grass, 0.5 kg / mu of festuca sinensis and 0.5 kg / mu of poa pratensis. The invention further provides a method for performing ecological restoration on the nymph alpine wetland by using the seed bag, no-tillage reseeding is performed in the middle ten days of May, and a rake is used for loosening soil and covering seeds after seeding. According to the method, the specific seeds are planted on the nymph alpine wetland according to the specific mass ratio, the ecological environment of the nymph alpine wetland can be improved, changes of soil pH, microorganisms and the like are supplemented, and the ecological system can be rapidly restored.

This invention discloses a lethal gene related to soybean thrips, MuαCOPI, and its application in the control of soybean thrips. This invention provides a lethal gene related to soybean thrips, MuαCOPI. Feeding soybean thrips with the dsRNA targeting MuαCOPI significantly increases the mortality rate. Further analysis using a combination of dsMuαCOPI and Serratia marcescens to feed soybean thrips resulted in a corrected mortality rate of approximately 88% for second-instar nymphs and approximately 52% for adults within 7 days. Synergistic virulence index analysis showed that the combined treatment of second-instar nymphs and adults with dsMuαCOPI and HvSm-1 produced a synergistic effect in virulence. This invention provides a safe and efficient method for the control of soybean thrips, with promising application prospects.

The utility model discloses a parasitic wasp vital activity observation device, which relates to the technical field of insect observation devices and comprises a leaf nutrition supply device, a parasitic wasp parasitism device and a plant leaf floating moisturizing device. The leaf nutrition supply device comprises a centrifugal tube capable of containing a nutrient solution and a leaf containing bemisia tabaci eggs or nymphs, the leaf is provided with a leaf stalk, the leaf stalk can be wrapped with degreasing cotton, and the degreasing cotton can be fixedly placed in the centrifugal tube; the parasitic wasp parasitism device comprises a culture dish capable of containing hydromel and an upper cover capable of covering the culture dish, and the leaf nutrition supply device can be placed in the culture dish; the plant leaf floating moisturizing device comprises a tray and a floating plate, a culture solution is contained in the tray, the floating plate can float on the culture solution, a centrifugal tube can be inserted into the floating plate, and the culture solution can be immersed into the centrifugal tube. The parasitic wasp life activity observation device is convenient to observe and high in experiment efficiency.

The method comprises the following steps that an incubation container containing egg masses is placed in an indoor environment suitable for egg incubation to be cultured, a moisturizing medium is arranged at an opening of the container, and moisturizing is conducted regularly to prevent the eggs from being wizened; after the arma chinensis nymphs are hatched, nutritional liquid is adopted for supplementary feeding every day; after the nymphs grow to 2 years old, transferring the nymphs into a medium-sized feeding container, controlling the density of the nymphs, meanwhile, inoculating inchworm larvae at the corresponding age as preys, and adding fresh plant branches and leaves into the container for inhabiting and obtaining water; after the nymphs grow to 4 years old, transferring the nymphs into a large feeding container, and feeding inchworm larvae at the adaptive age until the nymphs grow into adults; after adult copulation is found, an egg laying medium is placed, egg masses are collected, and the egg masses are used for next-generation breeding. According to the method, stable and efficient indoor fine breed breeding and feeding of arma chinensis can be achieved.