114 results about "Nymph" patented technology

Methods are provided for evaluating the insect resistance of a plant of interest. Specifically, methods are provided for high-throughput screening of soybean plants for resistance to kudzu bugs and stink bugs, including the brown marmorated stink bug and the southern green stink bug. In some embodiments, infesting emergent soybean seedlings with second instar stink bug nymphs and maintaining the nymphs in a closed environment with the seedling for only about 7 days allows evaluation of the stink bug resistance of a soybean variety of interest. Also provided are insect resistant plants, particularly plants resistant to kudzu bugs and stink bugs, as well as seeds produced by the resistant plants. Plants disclosed herein can be used to transfer the resistant trait into plant lines of interest.

The invention belongs to the field of insect cultivation and specifically relates to a scale breeding method of Scolothrips takahashii. The method includes peanut leaf selection, tetranychidae introducing, tetranychidae breeding, Scolothrips takahashii female imago introducing, Scolothrips takahashii female imago spawning, spawn collection and preservation, nymph development, preservation of 3rd instar nymphs. The scale breeding method is an imitating ecotype breeding method and is simple in production process, low in material costs and high in production efficiencies; the scale breeding method has a standardized operation procedure, and circulation supply of thrips foodstuffs can be achieved through one-time tetranychidae introducing; the obtained Scolothrips takahashii is orderly in developmental duration, high in rates of survival, high in activity and high in operability in application when the Scolothrips takahashii is used as natural enemy products; compared with general acaricide, the Scolothrips takahashii has the advantages of being small in labor intensity, high in efficiencies and good in control effects of tetranychidae and can not pollute the environment; and obtained agricultural products are free of pesticide residuals, and the Scolothrips takahashii can be widely applied to prevention of the tetranychidae in the field and has broad market prospects and obvious economic benefits.

The invention discloses a scale breeding method for encarsia formosa. The scale breeding method is finished in four greenhouses, namely a host plant cultivating room, a worm species room, an encarsia formosa species room and a breeding room, by steps. The scale breeding method comprises a host plant cultivating and managing step, a whitefly species managing step, a whitefly inoculating step, a whitefly nymph breeding step, an encarsia formosa species managing step, an encarsia formosa inoculating step, an encarsia formosa pupa breeding step, a pupa collecting step, a worm card or encarsia formosa box manufacturing and storing step and a whitefly controlling step. The scale breeding method has the advantages that process steps are continuously circulated and the encarsia formosa can be annually produced and bred. The scale breeding method is suitable for industrial scale production and reproduction of the encarsia formosa and is short in reproduction period, high in efficiency, low in energy consumption and low in cost. The obtained adult encarsia formosa, encarsia formosa pupa card or encarsia formosa box products are good in quality. The problem of insufficient supply for control over bemisia tabaci and trialeurodes vaporariorum in agriculture and forestry is solved.

The invention relates to an insect breeding bottom and a breeding method of gynaikothrips ficorum and belongs to the field of insect breeding and insect ecology. A bottom body is in a cone frustum shape with large top and small bottom and is in sealing match with a bottle cover, a magnifying glass is installed at the center of the bottle cover, a plurality of venting holes are evenly distributed on the bottle cover, and a filter screen is installed on the venting holes. The method utilizing the insect breeding bottom to breed the gynaikothrips ficorum comprises (1) collection of eggs, (2) hatching of the eggs, (3) breeding of nymphs and (4) breeding of imagoes. By using the breeding method, the hatching rate, survival rate and eclosion rate of the gynaikothrips ficorum are high, massive thrips of various insect states which are orderly in age and high in vigor can be obtained, and form changes and the whole life history of the gynaikothrips ficorum can be observed in real time.

The invention provides a method for preserving rice black-streaked dwarf virus (RBSDV) indoor living bodies. According to the principle of the method, wheat serves as a virus source host, small brown planthopper imagoes carrying the RBSDV spread the virus on wheat seedlings, a descendant small brown planthopper nymphs obtain the virus from the wheat, and accordingly a large of small brown planthoppers with the virus are obtained at a short period under indoor feeding conditions. The method effectively overcomes the technical bottleneck in preserving a large number of RBSDV indoor living bodies in a long term, and a series of technical problems that an RBSDV virus source is difficult to preserve continuously through the living bodies, the conventional cycle of obtaining the virus source is long, virus feeding plants cannot be provided for a long term, and minimum of the instar of the virus feeding nymphs is difficult to operate, and the like are solved. The obtained small brown planthoppers with the virus can support rice and corn variety resistance identification at any time and can also serve as RBSDV purification and serum preparation materials, and the problems that materials are difficult to obtain and the virus content is low and the like in conventional purification are solved. By application of the method, research, development and utilization of the RBSDV are greatly accelerated.

The invention provides a rapid and high-efficient artificial breeding method of golden cicada, and relates to a special animal breeding technology. The rapid and high-efficient artificial breeding method of golden cicada includes steps of collecting seed sources; pretreatment of egg branches; egg breeding: building up a green house, and placing egg branches in the house; artificial incubation: simulating the seed egg incubation process of the nature world, and providing a high-temperature and high-humidity incubation environment; preparation of breeding seeds; seed feeding: feeding egg branches at night; binding and hanging three or five of egg branches on a tree trunk, and making the incubated small nymphs climb down along branches (trunk) and drill in the soil; heat insulation. The rapid and high-efficient artificial breeding method can simulate the seed egg incubation of the nature world, provide high-temperature and high-humidity incubation environment and prompt the seed eggs to incubate and produce as soon as possible; for the wild golden cicada egg has dormancy in overwintering due to the low temperature, the incubation process can be completed for 1-2 years; however, golden cicada can be incubated for about 35 days according to the suitable condition provided by the invention, thus the breeding cycle is shortened by 1-2 years, and the incubation and production rate of seed eggs can reach over 95%, and is about 3 times higher than that of the nature world.

The invention discloses a method for increasing lipaphis erysimi breeding efficiency. The method for increasing the lipaphis erysimi breeding efficiency adopts a temperature changing mode to breed lipaphis erysimi and has the advantages that individuals of third-instar nymphs and imagoes of the lipaphis erysimi are larger than those of same-instar nymphs and imagoes in the prior art; 2, the survival rate of the imagoes of the lipaphis erysimi is increased, and the development duration of the lipaphis erysimi from spawns to the imagoes is shortened; 3, the life of lipaphis erysimi female imagoes generated through breeding is longer than those bred through a conventional mode, the lipaphis erysimi imagoes bred in a temperature changing mode are used for breeding next-generation lipaphis erysimi, and the spawning amount of female lipaphis erysimi is higher than that of lipaphis erysimi female imagoes bred through the conventional mode.

The invention relates to isaria fumosoroseus and application thereof for preventing and treating bemisia tabaci. The isaria fumosoroseus is preserved in China Center For Type Culture Collection, wherein the preserving data is 12th, October, 2012, and the preserving number is CCTCC M2012400. The taxonomic status belongs to Eumycota, Mitosporic fungi, Hyphomycetes, Hyphomycetales, Hyphomycetaceae and Isaria. The isaria fumosoroseus is highly pathogenic to B type bemisia tabaci, and the medial lethal concentration to two-year old nymph (LC50) is 7.5*10<5> conidia / mL (the fourth day after inoculation), and the field control efficiency to B type bemisia tabaci is over 90% which is equal to that of chemical insecticide spirotetramat. The isaria fumosoroseus serving as a living biological insecticide is free from environmental pollution and toxicity to human and livestock, and is suitable for production of organic agricultural products.

The invention provides a method for artificial infestation of Odontothrips loti on alfalfa, wherein the method for artificial infestation of Odontothrips loti nymphs comprises placing alfalfa newly expanded leaves in culture dishes, inoculating Odontothrips loti female imagoes, sealing the culture dishes with perforated fresh-keeping bags, culturing under certain conditions to obtain Odontothrips loti nymphs, and placing nymphs together with the newly expanded leaves to alfalfa plant top parts according to the required insect number of infestation for an individual alfalfa plant. The method for artificial infestation of Odontothrips loti imago comprises putting alfalfa young shoots in a wide-mouthed bottle, inoculating Odontothrips loti imagoes, directly applying on alfalfa plants to be infested, and keeping the wide-mouthed bottle near the plants for a moment. The method of the invention has the following effects: the cost of materials is low, the method is simple, the obtained Odontothrips loti imagoes are uniform and highly viable, the escape rate and the mortality rate of imagoes and nymphs are very low, and the controllability is high. The method is suitable for artificial infestation in a wide range.

The invention discloses an artificial feeding method for diaphorina citri. The method includes the following steps that feed plant cultivation and management are conducted, wherein feed plants include murraya paniculata and citrus; woodlice are fed; a woodlouse feeding cage is cleaned; and the feed plants are repaired. According to the method, a large number of woodlouse imagoes and nymphs not carrying Candidatus liberobacter asiaticus can be bred, and a large number of indoor woodlouse insect sources not carrying germs can be provided for areas where few woodlice or no woodlouse exists so as to meet needs of relevant tests.

The invention discloses an artificial cultivation method of cordyceps sobolifera. The method comprises the following steps: soaking mature nymphs of cicadas obtained by either field acquisition or artificial cultivation with pure water for 5 min, washing, and drying on a filter screen; uniformly spraying paecilomyces cicadae strain diluted solution, of which the total active bacteria number is 1*108, obtained by the culture and expanding propagation in a culture chamber, to the dried nymphs by a sprayer according to 5% of the weight of the nymphs; moving the re-dried nymphs into a cordyceps sobolifera cultivation plate, covering a plate cover, and cultivating in a sterile room at the temperature of 24-26 DEG C and the relative air humidity of 80-90%; after the heads of the nymphs grow cordyceps sobolifera which is similar to flowers, and taking out and drying the cordyceps sobolifera to obtain the artificially cultivated cordyceps sobolifera. Meanwhile, the invention discloses a cultivation plate for artificially cultivating the cordyceps sobolifera; in addition, by adopting the artificial cultivation method of cordyceps sobolifera to cultivate the cordyceps sobolifera, the industrial production of the cordyceps sobolifera can be realized, and the increasingly grown dietary therapy and clinical demands can be met.

The invention discloses a method for luring, catching and killing citrus black cicadas. A feasible method for luring, catching and killing nymphs does not exist at present. The method comprises the steps of laying straws and applying barnyard manure in furrows around citrus trees or pomelo trees according to life habits of black cicada larvas and nymphs, creating appropriate soil conditions and living environments and then luring, catching and killing the nymphs. The luring rate is improved by more than double through comparison. Simultaneously, the catching and killing time and measures of lured nymphs are limited and stipulated strictly. The yield of citrus orchards (or pomelo orchards) and nearby citrus orchards is improved by 19%-28.5% through comparison after the nymphs are lured, caught and killed. Furthermore, the income gained by selling the nymphs can be increased, and the economic benefit is obvious.

The present invention belongs to the technical field of biological pesticides, and specifically discloses paecilomyces lilacinus PlTS01 strain and application thereof in bemisia tabaci control. The paecilomyces lilacinus PlTS01 strain is isolated and purified from soil in Zhongchuan town Yongdeng County in Gansu Province, and is preserved in China Center for Type Culture Collection (CCTCC) on March 31, 2015, and the accession number is CCTCC No. M2015168. The paecilomyces lilacinus PlTS01 strain has strong bemisia tabaci insecticidal activity, sixth day after the treatment, medial lethal concentration LC50 of B-type bemisia tabaci second instar nymphs is 1.66 * 10<6> spores / mL, and the median lethal time LT50 is 4.35 d (at a dose of 1.0 *10<7> spores / mL). The paecilomyces lilacinus PlTS01 strain shows good prospects for being used as an environmentally friendly microbial pesticide in control of the agricultural important pest-bemisia tabaci.

An integrated hook and a nymph form shaped weight. The nymph form shaped weight is formed on the hook. The weight of the nymph form shaped weight is proportioned to a size of the hook. A method includes providing a fishing hook and forming a nymph form shaped weight directly on the hook. The nymph form shaped weight is sized proportionally to a size of the hook.

The invention provides a screening method of cotton varieties or strains resisting Bemisia tabaci. The method comprises artificially inoculating the Bemisia tabaci onto cottons of different varieties, selecting leaves with heaviest Bemisia tabaci damage on each cotton plant as standard leaves after 10-15 days of inoculation, counting numbers of adults, nymph and pupa of the Bemisia tabaci on the standard leaves, respectively representing with X, Y and Z, counting value zones of X, Y and Z, and selecting cotton varieties or strains with relatively few X, Y and Z as the cotton varieties or strains resisting the Bemisia tabaci. The method is relatively strong in practicality and maneuverability, and easy to operate, and can be used for selecting the cotton varieties or strains resisting the Bemisia tabaci.

The invention discloses a brown planthopper protein translation elongation factor NlEF1gamma, an encoded protein, synthesized dsRNA, and an application thereof in controlling brown planthopper pest diseases. The gene performs an important function in maintaining the normal growth and development processes of brown planthoppers. When the function is inhibited, brown planthopper nymph growth is slower, brown planthopper nymph size is smaller, and brown planthopper nymph dies because it couldn't develop into adult. When the function of the gene in a brown planthopper female adult is inhibited, its ovaries stop developing and it couldn't spawn normally. According to the invention, brown planthoppers are injected and fed with the dsRNA of the gene fragment. With both means the brown planthopper death rates reach 70% and brown planthopper spawning amounts are 0. Sequence and data bases are provided for establishing novel strategies for controlling pests with an RNA interference technology. The application has significant lethal and ovarian development inhibition effects against brown planthoppers. Therefore, the gene can be sued as an effective target for controlling the pest with an RNAi technology.

The invention belongs to the technical field of insect breeding, and discloses an indoor subculture breeding method of phenacoccus solenopsis tinsley. The method comprises the following steps of collecting and bringing back plants damaged by the phenacoccus solenopsis tinsley in the wild, and carrying out outdoor net room culture on the plants; after egg laying and primary incubation of the phenacoccus solenopsis tinsley, lightly or adhesively picking newly-incubated nymphs onto leaves of a host plant by using a soft writing brush by taking sticktight as the host plant, cutting off and treating the leaves, and then putting the leaves into a beaker; paving wet filter paper at the bottom of the beaker, and culturing in an incubator; after the newly-incubated nymphs of the phenacoccus solenopsis tinsley are molted, connecting the molted nymphs to fresh and clean single gendarussa ventricosa leaves, and placing the single gendarussa ventricosa leaves in the incubator. According to the method, the phenacoccus solenopsis tinsley on outdoor plants is cultured, obtained eggs are transferred into a room to be cultured, and a proper culture environment can be provided for nymph growth; in the indoor culture process, different culture conditions are set according to the growth period, culture is more targeted, long-term indoor subculture propagation of insects is facilitated, vitality iskept, and development of scientific experiments is facilitated.

The invention relates to the field of pest control technique of forest and fruit industries, and provides a method for controlling almond scale insects. The method comprises the following steps: evenly spraying a defoliant on a crown of an almond tree during a period between the last ten days of September and the first ten days of October, wherein, the defoliant is prepared by diluting 1ml of 40% etephon aqua with 200-300 times of water by volume. In the method, a plant growth regulator is used for reducing the base number of overwintering pests by making use of a habit that second-instar nymphs of the almond scale insects transfer from leaves to branches to live through the winter, thus achieving control on the damages of the almond scale insects, changing the traditional method and a period of controlling the almond scale insects by an insecticide, producing environmental pollution, causing no pesticide resistance of the pests and avoiding mistaken killing of the natural enemies of the pests.

The invention relates to an acaricidal composition, in particular to an acaricidal composition containing spirodiclofen and cyflumetofen, which is particularly used for preventing panonychus citri, European red mites, European red two-spotted spider mites and cotton leaf mites. The acaricidal composition has an obvious synergistic effect on mite killing as compared with a single pesticide, can kill egg, larva, nymph and adult of acarid, and is contributed to postponing the generation of pesticide resistance of bacteria.

The invention discloses a migratory locust nymph attractant as well as a lure and application thereof. The migratory locust nymph attractant is prepared from 2-heptanone and paraffin oil, the lure ofthe migratory locust nymph attractant is prepared by the following steps: uniformly mixing the paraffin oil with the 2-heptanone to obtain the migratory locust nymph attractant; soaking the rubber strip in the migratory locust nymph attractant for 1-2 hours, drying the rubber strip in the shade, and sealing the rubber strip with plastic to obtain the lure. The attractant is a preparation capable of adjusting migratory locust behaviors and can be used for controlling the migratory locust behaviors and preventing, controlling and monitoring migratory locust population quantity dynamics. 2-heptanone is a migratory locust metabolite, so that the attractant has no toxic effect on the environment and human, livestock and poultry, is safe to natural enemies, and belongs to a natural pesticide.

The invention relates to a method for inducing the wing shortening of Sogatella furcifera by utilizing an exogenous hormone, namely ZR-515 solution. The adopted technical scheme comprises the following steps of: freezing nymphs of the Sogatella furcifera at 3 to 4 ages, then dripping a small amount of juvenile hormone analogue, namely the ZR-515 solution on the backs of the nymphs, and raising the treated nymphs in a raising container for 2 to 8 days. The invention aims to design a novel, simple and convenient scheme for inducing the wing shortening of the Sogatella furcifera to improve the acquisition of short-winged individuals in the process of raising for experimental research materials, or provide a possible way for applying the method to fields and controlling the migration and dispersion hazard of the Sogatella furcifera in the future.

The invention discloses a large-scale production method of tamarixia radiate. The method comprises the steps that s1, carrier plants are managed, tender shoots are cultivated, and the carrier plants are calamondin; s2, diaphorina citri is inoculated to the carrier plants in a diaphorina citri breeding cage, and nymphs are bred; s3, the carrier plants with the 4-5-age diaphorina citri nymphs are moved into a parasitic wasp breeding cage, tamarixia radiate adults are put into the parasitic wasp breeding cage, the inoculation quantity ratio of tamarixia radiate to the diaphorina citri nymphs is 1: 10-20, and the tamarixia radiate adults are bred and collected; and s4, the carrier plants are cleaned and recovered. The carrier plants developed by the method are calamondin, the carrier plants shorten the growth cycle, the breeding capacity of the diaphorina citri and the production capacity of parasitic wasps are greatly improved, and the net yield and the female ratio of the tamarixia radiate are effectively improved according to the inoculation proportion of calamondin+tamarixia radiate to the diaphorina citri nymphs.

A method is provided for evaluating a protected plant having a protection mechanism. A population of immature insects (i.e., larvae or nymphs) belonging to an insect species having a plurality of instars is first exposed to the protected plant such that the protected plant is at least contacted thereby. The insect exposure extends for a selected time period corresponding to a sublethal exposure of the insect population to the protected plant. The exposure of the insect population to the protected plant is then halted following the selected time period and insects collected. A physical characteristic of at least some collected insects is measured and the immature insects are then sorted into instars. The measured physical characteristics are then statistically analyzed across the instars so as to determine an efficacy of the protection mechanism of the protected plant with respect to the insect population. Associated methods are also provided.

The invention relates to the technical field of insect breeding, in particular to a method for reducing the mortality rate of low-age nymphs in breeding of orius sauteri. The method is characterized in that gel and filter paper are matched for moisturizing bean sprouts and replenishing water and honey to orius sauteri, so that water is not required to be added in the breeding process of first-agenymphs and second-age nymphs; meanwhile, the bean sprouts can be kept fresh for a long time (one week or longer), and the bean sprouts can grow into leaves again, so that plant nutrition and moistureare provided for the orius sauteri. Therefore, the breeding method which is low in cost, convenient to operate, suitable for large-scale production and capable of effectively reducing the mortality rate of the low-age nymphs of the orius sauteri can be created according to the biological characteristics and growth and development requirements of the low-age nymphs.

The invention relates to the technical field of agriculture, in particular to a biological prevention and control method utilizing cucumis melo as a trap plant to prevent and control scale insects ofa citrus orchard. The method includes following steps: accelerating germination of cucumis melo, and transplanting into the citrus orchard after greenhouse seedling culture; in the citrus orchard, foran area where cucumis melo needs to be cultivated, ploughing, sufficiently watering, covering with thin films, and cultivating cucumis melo around and between rows of the citrus orchard area, managing water and fertilizer according to those of the citrus orchard, and managing cultivation according to cucumis melo planting technology; utilizing cucumis melo to attract the scale insects, and when damaged area of whole-plant fruits, leaves and vines of cucumis melo exceeds four fifth, bagging whole damaged cucumis melo plants, putting the same onto an open cement floor for exposure to the sun for several days, and burning up. The scale insects of the citrus orchard are attracted by utilizing cucumis melo and then treated in a centralized manner, so that the number of nymphs is reduced effectively, adults and eggs difficult to prevent and control are taken into consideration, and a new way is provided for efficient prevention and control of citrus scale insects.

The invention relates to the technical field of breeding device equipment, in particular to a breeding device for Riptortus pedestris. The breeding device for the Riptortus pedestris comprises a top box and a bottom box which are arranged in a penetrating mode, wherein an adult breeding assembly and a separation assembly are arranged in the top box, and an egg breeding assembly is arranged in the bottom box; the adult breeding assembly comprises an insect breeding cage, a limiting cage and a net plate, wherein the limiting cage sleeves and abuts against the bottom of the insect breeding cage, and the net plate is horizontally arranged below the limiting cage; two control pieces are mutually symmetrical and are correspondingly arranged between the adult feeding assembly and the inner wall of the top box; the separation assembly is arranged below the net plate; the egg breeding assembly comprises a nymph cage and a culture dish, and wet filter paper is laid on the inner bottom wall of the culture dish. According to the breeding device for the Riptortus pedestris, during breeding of the Riptortus pedestris, eggs and adults of the Riptortus pedestris are cultured in a separated mode, and an adult cage is effectively cleaned, so that an excellent growth environment is provided for the adults and the eggs of the Riptortus pedestris, and then the yield of the Riptortus pedestris is effectively increased.

The invention discloses a feeding device and method of Diaphorina citri. The feeding device comprises an insect-proof meshed bag, a food source plant cultivation pot and an outer pot; the insect-proofmeshed bag sleeves a top opening of the food source plant cultivation pot, the food source plant cultivation pot and the food source plant cultivation pot jointly form a Diaphorina citri cultivationchamber, and the food source plant cultivation pot is placed in the outer pot. The feeding device is a multifunctional integrated device suitable for collection, transport, isolated feeding and observation of Diaphorina citri samples, as well as cultivation management of seedlings; the feeding device may meet the need for alternating-generation feeding of adults and nymphs of Diaphorina citri andthe need for long-term continuous experimental observation, can provide simple feeding and observation of Diaphorina citri, controllable weather conditions and interference prevention, and is simple to operate and convenient to use.

A method for artificially breeding Stephanitis chinensis Drake comprises the following steps: collecting Stephanitis chinensis Drake nymphs, feeding the Stephanitis chinensis Drake nymphs to the egg laying early stage of adults, inoculating living tea tree seedling leaves planted by a root controller with adults which begin to lay eggs to for egg laying, removing the adults after egg laying, putting the tea tree seedlings loaded with Stephanitis chinensis Drake eggs into an incubator for incubation, and after the Stephanitis chinensis Drake eggs are incubated into nymphs, putting the nymphs into a constant-temperature culture dish to be fed into adults. According to the method, the egg period time of the Stephanitis chinensis Drake is effectively shortened, the average egg period is 43-46 days, the egg period is increased to 5 generations per year from 2-3 generations per year in the nature, the incubation rate of the bred Stephanitis chinensis Drake eggs reaches up to 89.77%, the survival rate of nymphs reaches 69.1% in the process that the nymphs are bred into adults, the average egg laying amount of the adults reaches 43.64 eggs per female insect, the nymphs and the adults are robust, the biological consistency is high, and as an experimental population, a research basis is provided for the exploration of subsequent effective prevention and control measures.

The invention discloses a large-scale arma chinensis fallou artificial breeding method. The method comprises the following steps that A, adults are mated and lay eggs; B, an egg mass is hatched; C, 2-5-age nymphs are fed; D, the adults are fed and released. The inventor dedicates to research the biological characteristics, the predatory function and artificial feeding of arma chinensis fallou fora long time, and large-scale feeding of the arma chinensis fallou is achieved through small tests and pilot tests; by the end of 2017, more than one million pieces of arma chinensis fallou are obtained; the large-scale breeding method is efficient, the land is saved, the cost is low, and the method is convenient to popularize.