111 results about "Nymph" patented technology

Methods are provided for evaluating the insect resistance of a plant of interest. Specifically, methods are provided for high-throughput screening of soybean plants for resistance to kudzu bugs and stink bugs, including the brown marmorated stink bug and the southern green stink bug. In some embodiments, infesting emergent soybean seedlings with second instar stink bug nymphs and maintaining the nymphs in a closed environment with the seedling for only about 7 days allows evaluation of the stink bug resistance of a soybean variety of interest. Also provided are insect resistant plants, particularly plants resistant to kudzu bugs and stink bugs, as well as seeds produced by the resistant plants. Plants disclosed herein can be used to transfer the resistant trait into plant lines of interest.

The invention belongs to the field of insect cultivation and specifically relates to a scale breeding method of Scolothrips takahashii. The method includes peanut leaf selection, tetranychidae introducing, tetranychidae breeding, Scolothrips takahashii female imago introducing, Scolothrips takahashii female imago spawning, spawn collection and preservation, nymph development, preservation of 3rd instar nymphs. The scale breeding method is an imitating ecotype breeding method and is simple in production process, low in material costs and high in production efficiencies; the scale breeding method has a standardized operation procedure, and circulation supply of thrips foodstuffs can be achieved through one-time tetranychidae introducing; the obtained Scolothrips takahashii is orderly in developmental duration, high in rates of survival, high in activity and high in operability in application when the Scolothrips takahashii is used as natural enemy products; compared with general acaricide, the Scolothrips takahashii has the advantages of being small in labor intensity, high in efficiencies and good in control effects of tetranychidae and can not pollute the environment; and obtained agricultural products are free of pesticide residuals, and the Scolothrips takahashii can be widely applied to prevention of the tetranychidae in the field and has broad market prospects and obvious economic benefits.

The invention discloses a scale breeding method for encarsia formosa. The scale breeding method is finished in four greenhouses, namely a host plant cultivating room, a worm species room, an encarsia formosa species room and a breeding room, by steps. The scale breeding method comprises a host plant cultivating and managing step, a whitefly species managing step, a whitefly inoculating step, a whitefly nymph breeding step, an encarsia formosa species managing step, an encarsia formosa inoculating step, an encarsia formosa pupa breeding step, a pupa collecting step, a worm card or encarsia formosa box manufacturing and storing step and a whitefly controlling step. The scale breeding method has the advantages that process steps are continuously circulated and the encarsia formosa can be annually produced and bred. The scale breeding method is suitable for industrial scale production and reproduction of the encarsia formosa and is short in reproduction period, high in efficiency, low in energy consumption and low in cost. The obtained adult encarsia formosa, encarsia formosa pupa card or encarsia formosa box products are good in quality. The problem of insufficient supply for control over bemisia tabaci and trialeurodes vaporariorum in agriculture and forestry is solved.

The invention relates to an insect breeding bottom and a breeding method of gynaikothrips ficorum and belongs to the field of insect breeding and insect ecology. A bottom body is in a cone frustum shape with large top and small bottom and is in sealing match with a bottle cover, a magnifying glass is installed at the center of the bottle cover, a plurality of venting holes are evenly distributed on the bottle cover, and a filter screen is installed on the venting holes. The method utilizing the insect breeding bottom to breed the gynaikothrips ficorum comprises (1) collection of eggs, (2) hatching of the eggs, (3) breeding of nymphs and (4) breeding of imagoes. By using the breeding method, the hatching rate, survival rate and eclosion rate of the gynaikothrips ficorum are high, massive thrips of various insect states which are orderly in age and high in vigor can be obtained, and form changes and the whole life history of the gynaikothrips ficorum can be observed in real time.

The invention discloses a method for improving breeding efficiency of encarsia formosa. When bemisia tabaci nymphs are bred by adopting a variable-temperature mode, the method disclosed by the invention has the following advantages: 1, individuals of bemisia tabaci nymphs of 2-3 instar are bigger than nymphs of the same instar in the prior art; 2, the survival rate of the nymphs and adults of the bemisia tabaci of all instars is improved, and the time, from egg development to the nymphs and the adults, of the bemisia tabaci is shortened; 3, service lives of the bemisia tabaci adults produced by breeding are longer than bemisia tabaci adults bred by adopting a conventional mode; the bemisia tabaci adults obtained by variable-temperature breeding are used for breeding next generation bemisia tabaci; the egg laying amount of females is higher than that of female bemisia tabaci adults bred by adopting the conventional mode, and the parasitism rate of the encarsia formosa to the bemisia tabaci nymphs is improved. According to the method disclosed by the invention, by shortening the breeding time of an encarsia formosa host, the breeding efficiency of the encarsia formosa is improved.

The invention relates to isaria fumosoroseus and application thereof for preventing and treating bemisia tabaci. The isaria fumosoroseus is preserved in China Center For Type Culture Collection, wherein the preserving data is 12th, October, 2012, and the preserving number is CCTCC M2012400. The taxonomic status belongs to Eumycota, Mitosporic fungi, Hyphomycetes, Hyphomycetales, Hyphomycetaceae and Isaria. The isaria fumosoroseus is highly pathogenic to B type bemisia tabaci, and the medial lethal concentration to two-year old nymph (LC50) is 7.5*10<5> conidia/mL (the fourth day after inoculation), and the field control efficiency to B type bemisia tabaci is over 90% which is equal to that of chemical insecticide spirotetramat. The isaria fumosoroseus serving as a living biological insecticide is free from environmental pollution and toxicity to human and livestock, and is suitable for production of organic agricultural products.

The invention provides a method for artificial infestation of Odontothrips loti on alfalfa, wherein the method for artificial infestation of Odontothrips loti nymphs comprises placing alfalfa newly expanded leaves in culture dishes, inoculating Odontothrips loti female imagoes, sealing the culture dishes with perforated fresh-keeping bags, culturing under certain conditions to obtain Odontothrips loti nymphs, and placing nymphs together with the newly expanded leaves to alfalfa plant top parts according to the required insect number of infestation for an individual alfalfa plant. The method for artificial infestation of Odontothrips loti imago comprises putting alfalfa young shoots in a wide-mouthed bottle, inoculating Odontothrips loti imagoes, directly applying on alfalfa plants to be infested, and keeping the wide-mouthed bottle near the plants for a moment. The method of the invention has the following effects: the cost of materials is low, the method is simple, the obtained Odontothrips loti imagoes are uniform and highly viable, the escape rate and the mortality rate of imagoes and nymphs are very low, and the controllability is high. The method is suitable for artificial infestation in a wide range.

The invention discloses an artificial feeding method for diaphorina citri. The method includes the following steps that feed plant cultivation and management are conducted, wherein feed plants include murraya paniculata and citrus; woodlice are fed; a woodlouse feeding cage is cleaned; and the feed plants are repaired. According to the method, a large number of woodlouse imagoes and nymphs not carrying Candidatus liberobacter asiaticus can be bred, and a large number of indoor woodlouse insect sources not carrying germs can be provided for areas where few woodlice or no woodlouse exists so as to meet needs of relevant tests.

The invention discloses a method for luring, catching and killing citrus black cicadas. A feasible method for luring, catching and killing nymphs does not exist at present. The method comprises the steps of laying straws and applying barnyard manure in furrows around citrus trees or pomelo trees according to life habits of black cicada larvas and nymphs, creating appropriate soil conditions and living environments and then luring, catching and killing the nymphs. The luring rate is improved by more than double through comparison. Simultaneously, the catching and killing time and measures of lured nymphs are limited and stipulated strictly. The yield of citrus orchards (or pomelo orchards) and nearby citrus orchards is improved by 19%-28.5% through comparison after the nymphs are lured, caught and killed. Furthermore, the income gained by selling the nymphs can be increased, and the economic benefit is obvious.

The present invention belongs to the technical field of biological pesticides, and specifically discloses paecilomyces lilacinus PlTS01 strain and application thereof in bemisia tabaci control. The paecilomyces lilacinus PlTS01 strain is isolated and purified from soil in Zhongchuan town Yongdeng County in Gansu Province, and is preserved in China Center for Type Culture Collection (CCTCC) on March 31, 2015, and the accession number is CCTCC No. M2015168. The paecilomyces lilacinus PlTS01 strain has strong bemisia tabaci insecticidal activity, sixth day after the treatment, medial lethal concentration LC50 of B-type bemisia tabaci second instar nymphs is 1.66 * 10<6> spores / mL, and the median lethal time LT50 is 4.35 d (at a dose of 1.0 *10<7> spores / mL). The paecilomyces lilacinus PlTS01 strain shows good prospects for being used as an environmentally friendly microbial pesticide in control of the agricultural important pest-bemisia tabaci.

An integrated hook and a nymph form shaped weight. The nymph form shaped weight is formed on the hook. The weight of the nymph form shaped weight is proportioned to a size of the hook. A method includes providing a fishing hook and forming a nymph form shaped weight directly on the hook. The nymph form shaped weight is sized proportionally to a size of the hook.

The invention provides a screening method of cotton varieties or strains resisting Bemisia tabaci. The method comprises artificially inoculating the Bemisia tabaci onto cottons of different varieties, selecting leaves with heaviest Bemisia tabaci damage on each cotton plant as standard leaves after 10-15 days of inoculation, counting numbers of adults, nymph and pupa of the Bemisia tabaci on the standard leaves, respectively representing with X, Y and Z, counting value zones of X, Y and Z, and selecting cotton varieties or strains with relatively few X, Y and Z as the cotton varieties or strains resisting the Bemisia tabaci. The method is relatively strong in practicality and maneuverability, and easy to operate, and can be used for selecting the cotton varieties or strains resisting the Bemisia tabaci.

The invention discloses a brown planthopper protein translation elongation factor NlEF1gamma, an encoded protein, synthesized dsRNA, and an application thereof in controlling brown planthopper pest diseases. The gene performs an important function in maintaining the normal growth and development processes of brown planthoppers. When the function is inhibited, brown planthopper nymph growth is slower, brown planthopper nymph size is smaller, and brown planthopper nymph dies because it couldn't develop into adult. When the function of the gene in a brown planthopper female adult is inhibited, its ovaries stop developing and it couldn't spawn normally. According to the invention, brown planthoppers are injected and fed with the dsRNA of the gene fragment. With both means the brown planthopper death rates reach 70% and brown planthopper spawning amounts are 0. Sequence and data bases are provided for establishing novel strategies for controlling pests with an RNA interference technology. The application has significant lethal and ovarian development inhibition effects against brown planthoppers. Therefore, the gene can be sued as an effective target for controlling the pest with an RNAi technology.

The invention belongs to the technical field of insect breeding, and discloses an indoor subculture breeding method of phenacoccus solenopsis tinsley. The method comprises the following steps of collecting and bringing back plants damaged by the phenacoccus solenopsis tinsley in the wild, and carrying out outdoor net room culture on the plants; after egg laying and primary incubation of the phenacoccus solenopsis tinsley, lightly or adhesively picking newly-incubated nymphs onto leaves of a host plant by using a soft writing brush by taking sticktight as the host plant, cutting off and treating the leaves, and then putting the leaves into a beaker; paving wet filter paper at the bottom of the beaker, and culturing in an incubator; after the newly-incubated nymphs of the phenacoccus solenopsis tinsley are molted, connecting the molted nymphs to fresh and clean single gendarussa ventricosa leaves, and placing the single gendarussa ventricosa leaves in the incubator. According to the method, the phenacoccus solenopsis tinsley on outdoor plants is cultured, obtained eggs are transferred into a room to be cultured, and a proper culture environment can be provided for nymph growth; in the indoor culture process, different culture conditions are set according to the growth period, culture is more targeted, long-term indoor subculture propagation of insects is facilitated, vitality iskept, and development of scientific experiments is facilitated.

The invention relates to the field of pest control technique of forest and fruit industries, and provides a method for controlling almond scale insects. The method comprises the following steps: evenly spraying a defoliant on a crown of an almond tree during a period between the last ten days of September and the first ten days of October, wherein, the defoliant is prepared by diluting 1ml of 40% etephon aqua with 200-300 times of water by volume. In the method, a plant growth regulator is used for reducing the base number of overwintering pests by making use of a habit that second-instar nymphs of the almond scale insects transfer from leaves to branches to live through the winter, thus achieving control on the damages of the almond scale insects, changing the traditional method and a period of controlling the almond scale insects by an insecticide, producing environmental pollution, causing no pesticide resistance of the pests and avoiding mistaken killing of the natural enemies of the pests.

The invention discloses a migratory locust nymph attractant as well as a lure and application thereof. The migratory locust nymph attractant is prepared from 2-heptanone and paraffin oil, the lure ofthe migratory locust nymph attractant is prepared by the following steps: uniformly mixing the paraffin oil with the 2-heptanone to obtain the migratory locust nymph attractant; soaking the rubber strip in the migratory locust nymph attractant for 1-2 hours, drying the rubber strip in the shade, and sealing the rubber strip with plastic to obtain the lure. The attractant is a preparation capable of adjusting migratory locust behaviors and can be used for controlling the migratory locust behaviors and preventing, controlling and monitoring migratory locust population quantity dynamics. 2-heptanone is a migratory locust metabolite, so that the attractant has no toxic effect on the environment and human, livestock and poultry, is safe to natural enemies, and belongs to a natural pesticide.

The invention discloses a large-scale production method of tamarixia radiate. The method comprises the steps that s1, carrier plants are managed, tender shoots are cultivated, and the carrier plants are calamondin; s2, diaphorina citri is inoculated to the carrier plants in a diaphorina citri breeding cage, and nymphs are bred; s3, the carrier plants with the 4-5-age diaphorina citri nymphs are moved into a parasitic wasp breeding cage, tamarixia radiate adults are put into the parasitic wasp breeding cage, the inoculation quantity ratio of tamarixia radiate to the diaphorina citri nymphs is 1: 10-20, and the tamarixia radiate adults are bred and collected; and s4, the carrier plants are cleaned and recovered. The carrier plants developed by the method are calamondin, the carrier plants shorten the growth cycle, the breeding capacity of the diaphorina citri and the production capacity of parasitic wasps are greatly improved, and the net yield and the female ratio of the tamarixia radiate are effectively improved according to the inoculation proportion of calamondin+tamarixia radiate to the diaphorina citri nymphs.

The invention relates to the technical field of insect breeding, in particular to a method for reducing the mortality rate of low-age nymphs in breeding of orius sauteri. The method is characterized in that gel and filter paper are matched for moisturizing bean sprouts and replenishing water and honey to orius sauteri, so that water is not required to be added in the breeding process of first-agenymphs and second-age nymphs; meanwhile, the bean sprouts can be kept fresh for a long time (one week or longer), and the bean sprouts can grow into leaves again, so that plant nutrition and moistureare provided for the orius sauteri. Therefore, the breeding method which is low in cost, convenient to operate, suitable for large-scale production and capable of effectively reducing the mortality rate of the low-age nymphs of the orius sauteri can be created according to the biological characteristics and growth and development requirements of the low-age nymphs.

The invention relates to the technical field of breeding device equipment, in particular to a breeding device for Riptortus pedestris. The breeding device for the Riptortus pedestris comprises a top box and a bottom box which are arranged in a penetrating mode, wherein an adult breeding assembly and a separation assembly are arranged in the top box, and an egg breeding assembly is arranged in the bottom box; the adult breeding assembly comprises an insect breeding cage, a limiting cage and a net plate, wherein the limiting cage sleeves and abuts against the bottom of the insect breeding cage, and the net plate is horizontally arranged below the limiting cage; two control pieces are mutually symmetrical and are correspondingly arranged between the adult feeding assembly and the inner wall of the top box; the separation assembly is arranged below the net plate; the egg breeding assembly comprises a nymph cage and a culture dish, and wet filter paper is laid on the inner bottom wall of the culture dish. According to the breeding device for the Riptortus pedestris, during breeding of the Riptortus pedestris, eggs and adults of the Riptortus pedestris are cultured in a separated mode, and an adult cage is effectively cleaned, so that an excellent growth environment is provided for the adults and the eggs of the Riptortus pedestris, and then the yield of the Riptortus pedestris is effectively increased.

The invention discloses a feeding device and method of Diaphorina citri. The feeding device comprises an insect-proof meshed bag, a food source plant cultivation pot and an outer pot; the insect-proofmeshed bag sleeves a top opening of the food source plant cultivation pot, the food source plant cultivation pot and the food source plant cultivation pot jointly form a Diaphorina citri cultivationchamber, and the food source plant cultivation pot is placed in the outer pot. The feeding device is a multifunctional integrated device suitable for collection, transport, isolated feeding and observation of Diaphorina citri samples, as well as cultivation management of seedlings; the feeding device may meet the need for alternating-generation feeding of adults and nymphs of Diaphorina citri andthe need for long-term continuous experimental observation, can provide simple feeding and observation of Diaphorina citri, controllable weather conditions and interference prevention, and is simple to operate and convenient to use.

A method for artificially breeding Stephanitis chinensis Drake comprises the following steps: collecting Stephanitis chinensis Drake nymphs, feeding the Stephanitis chinensis Drake nymphs to the egg laying early stage of adults, inoculating living tea tree seedling leaves planted by a root controller with adults which begin to lay eggs to for egg laying, removing the adults after egg laying, putting the tea tree seedlings loaded with Stephanitis chinensis Drake eggs into an incubator for incubation, and after the Stephanitis chinensis Drake eggs are incubated into nymphs, putting the nymphs into a constant-temperature culture dish to be fed into adults. According to the method, the egg period time of the Stephanitis chinensis Drake is effectively shortened, the average egg period is 43-46 days, the egg period is increased to 5 generations per year from 2-3 generations per year in the nature, the incubation rate of the bred Stephanitis chinensis Drake eggs reaches up to 89.77%, the survival rate of nymphs reaches 69.1% in the process that the nymphs are bred into adults, the average egg laying amount of the adults reaches 43.64 eggs per female insect, the nymphs and the adults are robust, the biological consistency is high, and as an experimental population, a research basis is provided for the exploration of subsequent effective prevention and control measures.

The invention provides a culture method of periplaneta americana. The method concretely comprises the following steps of 1, ootheca selection, 2, ootheca hatching, 3, nymph transferring, 4, nymph feeding, 5, adult feeding, 6, adult oviposition, and 7, adult catching and killing. A culture system of the periplaneta americana comprises an oviposition box, a hatching box, a culture box and a catchingand killing box, wherein a periplaneta americana dispelling agent is contained in a dispel box arranged in the oviposition box; multilayer hatching trays are arranged in the hatching box; a feeding groove is formed in a raising box; an automatic temperature control and humidity control device is arranged in each of the oviposition box, the hatching box and the culture box; each automatic temperature control and humidity control device comprises a microprocessor, a temperature and humidity alarm, a temperature sensor and a humidity sensor; and a steam machine used for providing the controlledtemperature and the controlled humidity is arranged in each of the oviposition box, the hatching box and the culture box. The culture method can solve the problems of low survival rate, low growth speed and great individual differences of the periplaneta americana, and the problem of low content of medical ingredients.