17 results about "Nymph" patented technology

The invention relates to a bemisia tabaci gene BtCOX8 and application thereof, and belongs to the field of biological control. The gene BtCOX8 highly expressed in the bemisia tabaci is screened, dsRNA is synthesized for the gene, the dsRNA is dripped on the body surface of nymphs of the bemisia tabaci, the nymphs are obviously shrunken and wizened, are in an obvious dehydration state and show the characteristics of growth resistance and serious physiological function disorder, and the results show that the BtCOX8 is a potential efficient RNAi lethal target. The dsRNA synthesized by the invention can be used for biological control of bemisia tabaci, is safe to encarsia formosa and orius minutus which are natural enemies of the bemisia tabaci, has the advantages of environmental friendliness and the like, and has a good application prospect.

The artificial efficient breeding method comprises the following steps that S1, host plants are planted in a spawning room, and the host plants are gramineae plants or asparagus plants which are easy to plant and have developed root systems; s2, collecting mature golden cicada nymphs which come out of the ground and are not subjected to eclosion, putting the collected mature golden cicada nymphs into an artificial climate box for artificial eclosion, and after eclosion and bee emergence, selecting parent adults; s3, putting the adults into a mating cage of an oviposition room according to a male-female ratio for mating, wherein an adult luring device consisting of a male cicada chirp generating device and a sex pheromone trap is arranged in the oviposition room. By arranging the adult luring device composed of the male cicada chirp generating device and the sex pheromone trap, the mating rate of the male cicada and the female cicada is synergistically increased, and technical support is provided for subsequently increasing the breeding rate of a cicada provenance; by means of the breeding method, efficient artificial breeding of the golden cicada seed source is achieved, and a seed source resource is provided for large-scale breeding of the golden cicada.

The invention relates to the field of artificial feeding of insects, and particularly provides an indoor efficient propagation method for tadelcoris lucorum based on snap beans. According to the method, fresh and mature snap bean pods are used as feeding and spawning hosts of adult teardiocoris suturalis, and feeding is conducted under the indoor conditions that the temperature is 28 DEG C, the humidity is 80% and the photoperiod is 12 L: 12 D; the vigna unguiculata containing eggs is taken out for moisturizing culture, and after incubation, tender vigna unguiculata is supplemented in time for nymphs to eat. According to the method, by optimizing host selection and environmental conditions, the three-day egg laying amount of the female tapolygus lucorum on the snap beans reaches 129 or above, the egg hatching rate reaches 71.3%, and the survival rate of nymphs reaches 80% or above. The method is convenient in material taking, low in cost and simple to operate, can effectively solve the problems of difficulty in indoor breeding, low egg laying rate, low hatching rate and the like of the adelphocoris suturalis in the prior art, and is suitable for standard and large-scale breeding of an adelphocoris suturalis test insect source in a laboratory.

The present application relates to the field of microbial technology, and discloses a spore-producing Metarhizium anisopliae strain and its application in the prevention and treatment of western flower thrips. The Metarhizium anisopliae provided by the present application has a preservation number of CCTCC No. M 20252118, a fast growth rate, a large spore yield, a high spore germination rate, and is easy to be cultured and multiplied in large quantities indoors. The present application can be used as a biocontrol fungus for preventing and treating the invasive agricultural pest western flower thrips, has strong pathogenicity to the western flower thrips nymphs, is safe to the environment, is not easy to make the pests resistant, and has application value.

A kind of tea processing preparation process, it is related to tea processing technical field, solve the problem of different water content of tea fresh leaves due to different degree of sucking and biting of small green leafhopper adults and nymphs and different time interval from sucking and biting to picking.The preparation process includes fresh leaf pretreatment, collision green and aroma enhancement, steam + high hot air fixation, spreading, striping, drying, screening and picking, aroma enhancement and packaging.In the fresh leaf pretreatment process, small green leafhopper adults are put in, and small green leafhoppers are attracted to suck and bite fresh leaves by yellow light.In the collision green process, tea fresh leaves are made to jump by vibration to carry out collision green treatment.The present application solves the problem of different degree of fresh leaf water loss due to different degree of sucking and biting of small green leafhopper adults and nymphs and different time interval from sucking and biting to picking by putting small green leafhopper adults and nymphs to concentrate sucking and biting of tea fresh leaves.Meanwhile, the quality of cicada tea is effectively improved by temperature and humidity control, light supplement, collision green and aroma enhancement, steam + high hot air fixation.

The invention relates to the field of insect prevention and control, in particular to a high-toxicity metarhizium anisopliae strain IPPMA362 resistant to high temperature and ultraviolet stress and application thereof. The invention aims to screen excellent metarhizium anisopliae strains with high toxicity to bemisia tabaci. Through indoor biological activity determination, the lethal ability of the target strain on the bemisia tabaci nymphs is determined; further, key biological characteristics such as sporulation capability, spore germination rate and ultraviolet resistance of the strain are systematically evaluated, so that the application potential of the strain is judged; and the optimized strain is accurately identified through a molecular biology means, so that a foundation is laid for subsequent strain registration, patent protection and product development.

This invention relates to the field of artificial breeding technology for natural enemy insects, and in particular to a method for the artificial breeding of the assassin bug *Triplophysa pulcherrima*. The invention employs steps including seed source collection and screening, rearing environment control, feed supply and management, mating and reproduction management, oviposition and egg mass collection, nymph hatching and age-differentiated rearing, overwintering management, and cannibalism control. By providing specialized rearing management devices, temperature and humidity conditions, standardized feed feeding, age-differentiated rearing density control, and moisture-controlled egg mass hatching, it solves the problems of high mortality rates among young nymphs, unstable mating success rates, low egg mass hatching rates, and the lack of a year-round breeding system in existing technologies. The method of this invention is simple to operate, has a high survival rate (nymphal survival rate exceeding 85%), an egg mass hatching rate exceeding 90%, and can achieve 4-5 generations per year, enabling large-scale, year-round production of *Triplophysa pulcherrima*, providing high-quality natural enemy resources for the biological control of agricultural and forestry pests.

The invention relates to an azobenzene 4-trifluoromethyl nicotinamide derivative as well as a preparation method and application thereof, in particular to the azobenzene 4-trifluoromethyl nicotinamide derivative with a structure as shown in a formula I. The azobenzene 4-trifluoromethyl nicotinamide derivative has excellent insecticidal activity. The pesticide can effectively prevent and treat insects with piercing-sucking mouthparts such as alfalfa aphids, and especially has a very good prevention and treatment effect on one-day-old nymphs of alfalfa aphids.

This invention belongs to the field of pest control technology, and provides a sex pheromone for the tea mirid bug, its preparation method, and its application. The sex pheromones of the tea mirid bug of this invention are (R)-3-butoxybutyrate octyl ester, (S)-3-butoxybutyrate octyl ester, and (R)-3-butoxybutyrate hexyl ester. The sex pheromones of the tea mirid bug of this invention are non-toxic, do not need to be directly applied to tea trees, pose no pesticide residue risk to tea leaves, and are harmless to beneficial insects in tea gardens. They are highly effective in attracting and killing male tea mirid bugs, directly killing large numbers of male tea mirid bugs that damage tea leaves, disrupting the male-to-female ratio, reducing mating success rates, and indirectly controlling the next generation of nymphs from damaging tea trees. The slow-release lure made from the sex pheromone of this invention contains milligrams of the tea mirid bug's sex pheromone, making it highly efficient, and its effective period lasts up to 32 days. Long-term use can maintain long-term suppression of the male tea mirid bug population.

The application discloses a breeding method of Harpactor fuscipes and application thereof in prevention and control of mulberry pyralid, and belongs to the technical field of pest control. The application comprises the following steps: (1) capturing adult Harpactor fuscipes from the wild, feeding, oviposition, and then hatching and breeding the eggs; (2) 1st instar nymphs: from hatching to the first molting, a mixed solution is used to dip a cotton ball to obtain a mixed solution cotton ball, the mixed solution cotton ball is placed in a hatching box, and the hatched Harpactor fuscipes feeds by itself; (3) after 2nd instar: in addition to the mixed solution cotton ball in the step (2), the 2nd instar to 4th instar Harpactor fuscipes is fed with artificial feed of corresponding instar silkworm, and the 5th instar Harpactor fuscipes and adult Harpactor fuscipes is fed with 4th instar artificial feed of silkworm. The method has high breeding rate and low cost, and the bred Harpactor fuscipes has remarkable effect in application in prevention and control of mulberry pyralid.

The invention discloses a biological assay method for evaluating the influence of gene editing wheat on aphids, and belongs to the technical field of safety evaluation of gene editing crops. The bioassay method comprises the following steps: selecting a single wheat seedling after wheat seedlings emerge, wrapping the stem base of the single wheat seedling with wet absorbent cotton, fixing the stem base in a tube, then inoculating single-head wing-free adult aphids on the wheat seedling, removing the adult aphids and redundant young aphids after the adult aphids produce young aphids for the first time, and only retaining one young aphid. And from the beginning of the first-stage aphid production, observing once under a stereoscopic microscope every day, recording the growth and development conditions of the aphids, and removing aphid slough and dead individuals in time. And evaluating the influence of the gene editing wheat on the aphids by taking the survival rate of the aphids on the tenth day, the early aphid production stage, the life and the aphid production quantity as evaluation indexes. The method is wide in applicability and can be effectively used for evaluating the influence of various kinds of gene editing wheat on various aphids such as schizaphis graminum, sitobion avenae and rhopalosiphum padi.

The invention discloses a periplaneta americana breeding method which comprises the following steps: step 1, preparing equipment, using a modular breeding box, dividing the interior of the modular breeding box into four independent breeding areas, namely a larva area, a nymph area, an adult area and a spawning area, separating the areas through sealing partition plates, and arranging independent environment regulation and control modules, the environment regulation and control module comprises a temperature control module, a humidity control module and an illumination regulation and ventilation system; 2, seed insect screening: selecting American cockroach adults with no body surface damage, quick activity, no deformity and excrement formation, the weight of a single American cockroach adults being greater than or equal to 1g, the male-female ratio being 3: 1 and the putting density being 100-200 adults per square meter, disinfecting a culture box before putting, performing spray disinfection on the culture box by adopting a 0.5% peracetic acid aqueous solution before putting, and ventilating for 24 hours after disinfection, after no disinfectant is left in the box body, seed insects are put in. The invention relates to the technical field of breeding, and particularly provides a breeding method of periplaneta americana.

This invention discloses a lethal gene related to soybean thrips, MuαCOPI, and its application in the control of soybean thrips. This invention provides a lethal gene related to soybean thrips, MuαCOPI. Feeding soybean thrips with the dsRNA targeting MuαCOPI significantly increases the mortality rate. Further analysis using a combination of dsMuαCOPI and Serratia marcescens to feed soybean thrips resulted in a corrected mortality rate of approximately 88% for second-instar nymphs and approximately 52% for adults within 7 days. Synergistic virulence index analysis showed that the combined treatment of second-instar nymphs and adults with dsMuαCOPI and HvSm-1 produced a synergistic effect in virulence. This invention provides a safe and efficient method for the control of soybean thrips, with promising application prospects.

The utility model discloses a device and system for raising cantheconidea furcellata, and relates to the technical field of cantheconidea furcellata raising, the device comprises a breeding box and simulation vine branches, the side wall of the breeding box is provided with at least one ventilation opening, the ventilation opening is provided with a ventilation net, and the top and the side wall of the breeding box are fixedly connected with a plurality of simulation vine branches. The simulated vine branches are in staggered lap joint and can be uniformly distributed in the three-dimensional space of the breeding box, and the cantheconidea furcellata can climb to the top of the breeding box from the bottom of the breeding box along the simulated vine branches. The simulation vine branches are evenly distributed in the three-dimensional space of the breeding box, the space utilization rate of the breeding box is improved, the simulation vine branches can facilitate climbing and inhabiting of adults, the activity space in the breeding process of the cantheconidea furcellatus is increased, the number of the cantheconidea furcellatus in the unit volume of the breeding box is increased, and the breeding efficiency is improved. Therefore, the feeding and breeding efficiency of cantheconidea furcellata is improved, and the breeding cost is reduced.

This invention relates to the application of (E,E)-4,8,12-trimethyl-1,3,7,11-tetracene or myristyl acetate in attracting green mirid bugs. Specifically, (E,E)-4,8,12-trimethyl-1,3,7,11-tetracene has an attractive effect on nymphs, female adults, and male adults of the green mirid bug; furthermore, myristyl acetate can enhance the attraction of TMTT to nymphs, female adults, and male adults of the green mirid bug.

The application discloses a lethal related gene MuSrp54k of a bean leaf beetle and application of the lethal related gene MuSrp54k in prevention and treatment of the bean leaf beetle. The application provides a Serratia marcescens HvSm-1 capable of being used for prevention and treatment of the bean leaf beetle, and a lethal related gene MuSrp54k of the bean leaf beetle, and constructs a dsRNA-dsMuSrp54k for prevention and treatment of the bean leaf beetle by using the lethal related gene MuSrp54k. Further, after the bean leaf beetle is fed by using the dsMuSrp54k and the Serratia marcescens jointly, the corrected mortality of the 2nd instar nymphs is about 88% within 7 days, and the corrected mortality of the adults is about 58% within 7 days, and the synergistic virulence index is used to judge that the virulence of the 2nd instar nymphs and the adults of the bean leaf beetle treated by the dsMuSrp54k and the HvSm-1 jointly produces a synergistic effect. The application provides a safe and efficient method for prevention and treatment of the bean leaf beetle, and has a good application prospect.