100 results about "Diaphorina citri" patented technology

The invention discloses a diaphorina citri Kuwayama parasitism natural energy scale breeding method. Insects and germs on a Murraya exotica plant are eliminated to obtain a clean Murraya exotica without disease and pest infection. The clean Murraya exotica without the disease and pest infection is transferred to a first greenhouse or a first insect rearing cage, and the diaphorina citri Kuwayama is grafted to breed. Under normal lighting conditions, the Diaphorina citri Kuwayama is cultured and bred under the temperature of 22 to 28 DEG C, and the Murraya exotica or the Diaphorina citri Kuwayama is supplemented at any moment according to requirements. The clean Murryaya exotica without the disease and pest infection is transferred to a second greenhouse or a second insect rearing cage, and the Diaphorina citri Kuwayama in the first greenhouse or the first insect rearing cage is grated. Under the normal lighting conditions, the Diaphorina citri Kuwayama is cultured to spawn and grow to a nymph of 4-5 years old under the temperature of 22 to 28 DEG C. The parasitic wasp and parasitic Diaphorina citri Kuwayama are utilized for inoculation, and the parasitic wasp can be colleted after 7 to 10 days. According to the Diaphorina citri Kuwayama parasitism natural energy scale breeding method, the industrialization production of the Diaphorina citri Kuwayama parasitic wasp is achieved.

The invention relates to a paecilomyceslilacinus strain having strong pathogenicity for diaphorina citri and belongs to the technical field of the microorganism. The strain is paecilomyceslilacinus ZJPL08 which is registered and conserved in China General Microbiological Culture Collection Center on March 15, 2013, and the conservation number of the strain is CGMCC No. 7318. The paecilomyceslilacinus ZJPL08 provided by the invention has stronger pathogenicity for diaphorina citri and can be developed into a biocontrol agent for biological prevention and treatment of diaphorina citri; and compared with the present chemical agent for preventing and treating diaphorina citri, the paecilomyceslilacinus ZJPL08 has the characteristics of high efficiency, low toxicity, low residue, no pollution and not easy creation of resistance to drugs. The strain ZJPL08 has stronger acting effect on diaphorina citri than other paecilomyceslilacinus strains; the strain ZJPL08 is quick to cultivation, high in sporulation quantity, high in germination rate of spores and easy for preparation, and has excellent market application prospect.

The invention discloses a new method for surveying population density of microarthropods such as panonychus citri mcgregor, predatory mites and diaphorina citri kuwayama. The new method comprises the following steps of: randomly drawing a citrus sample plant; placing adhesive plate boxes below tree crowns in the east, south, west and north directions of the tree crown of the citrus sample plant; knocking branches of the tree crowns above the positions where the adhesive plate boxes are arranged for many times to drop the microarthropods; adhering the microarthropods falling in the adhesive plate boxes to adhesive plates of the adhesive plate boxes; collecting the adhesive plate boxes to perform statistical survey, wherein the adhesive plate boxes comprise box bodies; a layer of plate corresponding to the size of the bottom surface is arranged on the bottom surface of each box body; an adhesive material is coated on the upper surface of the plate; and thus, the adhesive plates are constructed. Thus, by surveying with the method provided by the invention, the used time is less, the sample can be stored for a long time, and most importantly, the detection sensitivity to the microarthropods of red spiders, predatory mites and the like is greatly improved; and since the detected objects are adhered and cannot escape, the detection is more convenient, simpler and more accurate.

The invention discloses a Diaphorina citri Kuwayama high pathogenic Isaria fumosorosea strain IF-BDC01 and application thereof. The Isaria fumosorosea strain IF-BDC01 is preserved in Guangdong Microbiology Culture Center on March 1, 2016 with a preservation number of GDMCC No:60011, and the preservation address is building 59, 100th yard on middle martyr road, Guangzhou. Long-term infection biology and indoor biological determination proves that the Isaria fumosorosea strain IF-BDC01 has very high pathogenicity on Diaphorina citri Kuwayama and very strong infecting insecticidal effect. The strain is an entomopathogenic fungus, and has very strong application potential as an in vivo biopesticide in biological control of Diaphorina citri Kuwayama.

The invention belongs to the technical field of control of fruit tree diseases and particularly discloses a method for screening citrus HLB (Huanglongbing) control agents through grafting. According to the method for screening the citrus HLB (Huanglongbing) control agents, a citrus scion infected with the disease is taken as an object, the content change of Candidatus liberibacter asiaticus in the scion infected with the disease and treated with the agents is detected quantitatively through a real-time PCR (polymerase chain reaction), and the control effects of the treating agents are evaluated on the basis. The citrus scion infected with the disease is taken as a study object directly, the agent efficacy evaluation more conforms to practical situations, and influence caused by an interhost such as Diaphorina citri Kuwayama or catharanthus roseus used in screening of Candidatus liberibacter asiaticus control agents is eliminated. The problems that a screening process of the Candidatus liberibacter asiaticus control agents is complicated, a reliable and effective evaluation system is absent and the like at present are solved, and reliable technical and theoretical bases are provided for control of citrus HLB.

The invention relates to the technical field of orange insect disease prevention and treatment and discloses a composition for trapping diaphorina citri and a trapping method using the same. The composition comprises gamma-terpinene, linalool, beta-caryophyllene and alpha-copaene. The trapping method comprises the following steps of putting the composition into a transparent plastic insect-trapping bottle, covering the bottom with a bottle cover, spraying an insect binder to the outer part of the insect-trapping bottle to obtain an insect binding bottle, hanging the insect binding bottle on a diaphorina citri host plant, and carrying out diaphorina citri trapping and binding. The composition can improve a trapping amount by 62 times in the case of high diaphorina citri density, has substantial trapping effects, can be used for preventing, treating and forecasting diaphorina citri, and can also be used for preventing citrus Huanglongbing propagated by diaphorina citri.

The invention discloses a planting method for controlling the infection of the citrus tristeza virus on newly-grown citrus seedlings. The planting method includes the steps that terrestrial citrus seedlings are loaded with a nutrition pot filled with nutrition soil or field soil, then the nutrition pot loaded with the terrestrial citrus seedlings is put into a greenhouse to be cultivated in a centralized mode or centralized in farmland to be cultivated, a black mulching film or a light reflecting mulching film is used for covering the ground of the farmland, the farmland is relatively independent, and citrus planting does not exist in the far peripheral range; the young shoot emerging time, the shoot emerging amount and the young shoot growing height of each batch are recorded; the pest and disease damage occurrence on the citrus seedlings is regularly detected, spraying preventing and controlling are conducted purposefully, a diaphorina citri killing pesticide is sprayed in the shoot stage, meanwhile, pests such as aphids and leaf miners are controlled, and disease preventing and controlling are conducted according to a conventional method; the tristeza virus is detected every year, and if trees infected with the tristeza virus are found, the tristeza virus infected with the tristeza virus are removed immediately; the citrus seedlings are fixedly planted into a field after two years. The planting method can effectively lower the risk of infecting the citrus tristeza virus and even eliminate the citrus tristeza virus.

The invention relates to the fields of prevention and treatment of citrus plant diseases and insect pests, and particularly relates to a composition for trapping diaphorina citri kuwayama and an application method of the composition. The composition comprises gamma-terpinene, alpha-pinene and linalool. The method comprises the following steps: putting the prepared composition into a transparent plastic trapping bottle; covering by a bottle cap, and spraying an insect sticker outside the trapping bottle to prepare an insect sticking bottle; and hanging the insect sticking bottle on a host plant of the diaphorina citri kuwayama to trap and stick the diaphorina citri kuwayama, wherein the trap quantity of the composition under the condition of high diaphorina citri kuwayama density can be increased by 49.3 times, and the trapping quantity at low diaphorina citri kuwayama density is also increased by 5.17 times. The trapping effect of the composition is significant; the composition can be applied to prevention, treatment and prediction of the diaphorina citri kuwayama; and meanwhile, the propagated citrus yellow shoot is prevented.

The invention discloses an efficient and comprehensive citrus tristeza virus prevention and control technology, and relates to the field of plant disease and insect pest prevention and control, in particular to a citrus tristeza virus prevention and control technology. The technology includes the following steps of firstly, rapidly cleaning an ill tree through a five-step method; secondly, replanting safe big seedlings heeled in a net house; thirdly, precisely and efficiently preventing and controlling diaphorina citri in the five key periods; fourthly, establishing a mechanism with overall prevention and treatment in large areas and 'central householder system' joint prevention and control in small areas; fifthly, planting a fir prevention and isolation belt. The citrus tristeza virus incidence rate of a fruit garden can be effectively controlled, economic loss is directly reduced, the income of fruit farmers is improved, meanwhile the pesticide prevention and treatment frequency andprevention and treatment cost for the diaphorina citri can be reduced, and therefore the pesticide surface source contamination is reduced, and the technology has remarkable economic, social and ecological benefits.

The invention discloses a method for radically treating citrus candidatus liberobacter. A solution for radically treating the citrus candidatus liberobacter is prepared and loaded in a suitable container, a needle tube of an injection syringe for trees is connected with the container, a needle is inserted into the rhizome of a citrus tree or the main root close to the ground surface, a liquid drainage hole in the syringe for the trees reaches the phloem and the xylem, and the solution for radically treating the citrus candidatus liberobacter is injected through the syringe for trees, wherein the solution for radically treating the citrus candidatus liberobacter contains any one or two of bactericidal solutions, one or more of diaphorina citri killing solutions and one or more of tree vigorimprovement solutions. The method for radically treating the citrus candidatus liberobacter has the advantages that the prevention and control of bacteria of the citrus candidatus liberobacter, the prevention and control of the diaphorina citri and the improvement of the tree vigor of diseased trees are organically combined, integrated prevention and control are conducted, and the effect is obvious.

The invention discloses application of a special light source in the aspect of diaphorina citri trapping and a diaphorina citri trapper. The wavelength of the special light source is 409.9-511.6 nanometers. Light of different wavelengths has different trapping rates for diaphorina citri trapping, the diaphorina citri has the high response index and the positive phototaxis index for the light source with the wavelength being 409.9-469.2 nanometers or 491.6-511.6 nanometers. When the light source of the wavelength is adopted, the effect of trapping diaphorina citri through lamplight can be greatly improved; meanwhile, the wavelength of the special light source is single, the pertinence is high, the diaphorina citri trapping specificity is high, and damage caused to other insects, particularly beneficial insects is small. The light source of the specific wavelength is adopted for trapping diaphorina citri, the effect of trapper lamplight trapping can be remarkably improved, the diaphorinacitri prevention effect of the trapper is improved, the consumption of chemical pesticide is lowered, pesticide residues on diaphorina citri are reduced, generation of chemical pesticide resistance of diaphorina citri is delayed, and contaminations of chemical pesticide to the environment are reduced.

The invention relates to an insect-killing composition suitable for drip irrigation to prevent and control diaphorina citri. The effective components of the insect-killing composition are binary compounding of sulfoxaflor and cyantraniliprole. According to indoor bioassay, the optimal synergic ratio of the insect-killing composition to diaphorina citri is 5:3. The insect-killing composition has the advantages that the insect-killing composition has an obvious synergistic effect; an insecticidal spectrum is expanded, and the pesticide resistance of pests is lowered; the pesticide dose is reduced; pesticide residues are lowered; meanwhile, the pesticide cost and labor force cost are saved. By means of the insect-killing composition suitable for drip irrigation to prevent and control diaphorina citri, the problems that agent is not uniformly sprayed when the agent is applied by being sprayed, phylloxera cannot be prevented and controlled at a hidden place, and the pesticide applying timeand the pesticide applying frequency cannot be controlled can be solved. With the assistance of an existing drip irrigation system, the composition is directionally applied to root areas of crops, through root systems, the composition is absorbed and transferred to tender parts of citrus leaves, and diaphorina citri can be directionally prevented and controlled.

The invention belongs to the technical field of pesticide and in particular discloses a medicine composition capable of preventing and treating diaphorina citri. The medicine comprises the following active compounds: acetamiprid and rotenone of which the mixing volume ratio is (5-15) to 1. By mixing of a specific ratio, the toxicity effects of the acetamiprid and rotenone to diaphorina citri can achieve a synergistic interaction action, thus realizing the effect of effectively preventing and treating diaphorina citri. In addition, by matching the two medicines for use, use concentration of the medicine can be reduced, cost is reduced and more importantly, harm to the plants is reduced.

The invention discloses a large-scale breeding method for tamarixia radiate. The breeding method comprises the following steps: performing pest species management on diaphorina citri, inoculating diaphorina citri, breeding diaphorina citri nymphs, performing bee species management on tamarixia radiate, inoculating the tamarixia radiate, breeding bee pupa of tamarixia radiate, and collecting the bee pupa. The large-scale breeding method for tamarixia radiate provided by the invention has the advantages of continuous cycle process steps and capacity of annually breeding, and is short in breeding period, high in efficiency, low in cost and low in energy consumption, the bred tamarixia radiate is high in quality, and the problem that supply of diaphorina citri is insufficient during control of agriculture and forestry is solved.

The invention discloses an artificial feeding method for diaphorina citri. The method includes the following steps that feed plant cultivation and management are conducted, wherein feed plants include murraya paniculata and citrus; woodlice are fed; a woodlouse feeding cage is cleaned; and the feed plants are repaired. According to the method, a large number of woodlouse imagoes and nymphs not carrying Candidatus liberobacter asiaticus can be bred, and a large number of indoor woodlouse insect sources not carrying germs can be provided for areas where few woodlice or no woodlouse exists so as to meet needs of relevant tests.

The invention discloses application of a soy sauce residue glyceride in preparation of a preparation for controlling minisize insects or foliar fungal and bacterial diseases. The soy sauce residue glyceride is a glyceride containing C16-C22 fatty acids. According to the invention, the soy sauce residue glyceride is taken as a main component, and effective auxiliary agents are added for preparationof a pesticide preparation. Through tests, the pesticide preparation has a same controlling effect on tetranychus urticae, diaphorina citri, panonychus citri, aedes albopictus and other minisize insects and sooty mould and other diseases compared with a current mineral oil emulsion, and does not show phytotoxicity after repeated application to crops for a short period of time. The pesticide preparation has the advantages of simple and stable ingredients, high safety, good anti-insect and disease-preventing effects, and no phytotoxicity to crops. The main components and additives of the pesticide preparation are food grade, and easy to degrade in environment, and the pesticide preparation is easy for industrialized standardized production, and has strong market competitiveness compared with mineral oil emulsions.

The invention belongs to the field of agricultural science and in particular relates to an artificial feeding method of diaphorina citri kuwayama. The method comprises the following steps: putting nutrient soil into a small transparent plastic cup, and culturing a diaphorina citri kuwayama host plant, wherein drainage holes are formed in the bottom of the small plastic cup; inoculating diaphorinacitri kuwayama with the host plant, covering the small plastic cup by using a large transparent plastic cup in an embedding manner, and performing culture under conditions that the temperature is keptto 28+/-0.5 DEG C, the relative humidity is 60+/-5% and the light cycle is 14L:10D, so as to obtain imagoes of diaphorina citri kuwayama, wherein vent holes are formed in the top of the covering large plastic cup. The artificial feeding method of the diaphorina citri kuwayama, which is provided by the invention, is simple and feasible, and is particularly applicable to indoor breeding, and the life history of the diaphorina citri kuwayama can be observed. By adopting the method, an insect source material can be provided for research and development of sex pheromone, screening of medicines, resistance monitoring and resistance risk evaluation on the diaphorina citri kuwayama, meanwhile, a breeding device is simple, the breeding cost is low, breeding host plants and the like can be repeatedly used for multiple times, and the cost can be reduced.

The invention belongs to the technical field of preventing and controlling of insects and pests, and particularly discloses a special sticking attractant for diaphorina citri. The special sticking attractant is prepared from the following raw materials in parts by weight: 8 to 12 parts of insect attracting agent, and 50 to 80 parts of insect sticker, wherein the insect attracting agent is preparedfrom the following raw materials in parts by weight: 2 to 4 parts of plant-sourced pheromone, 2 to 3 parts of insect pheromone, 10 to 15 parts of ethyl alcohol, 1 to 2 parts of chitosan, 0.8 to 2 parts of propolis, 8 to 15 parts of plant leaf extract, and 2 to 5 parts of konjac glucomannan. The special sticking attractant for diaphorina citri has the advantages that by utilizing the sensitivity of the diaphorina citri to light and terpene compounds, the raw materials are reasonably compounded, and the prepared sticking attractant has stronger killing ability on the diaphorina citri; by addingthe konjac glucomannan and the chitosan into the raw materials, the timeliness is longer, and the certain slow-release efficiency is realized.

The invention discloses a method for controlling diaphorina citri insect pests by interplanting citrus with guava, and relates to the technical field of agricultural planting. The method comprises thefollowing steps: citrus are planted by adopting a conventional method, wherein the line spacing of the citrus is 2.5-3.5 meters and the plant distance is 1.5-2.5 meters, so that a citrus garden is formed; guava is interplanted into citrus trees, one guava tree is interplanted every 5-7 citrus trees from the first citrus tree in every uneven row, and one guava tree is interplanted every 5-7 citrustrees from the last citrus tree in every even row; and the guava trees is 80-100 cm higher than the citrus trees through trimming, single rows of guava are planted around a citrus garden, and the plant distance of the guava trees is 1.5-2.5 meters. Citrus diseases and insect pests are prevented and controlled through plant diversity, diaphorina citri in the citrus garden is greatly reduced, costof spraying large amounts of pesticides is reduced, pesticide damage is avoided, infection risk of citrus huanglongbing disease is reduced, and remarkable economic, social and ecological benefits areachieved. Researches show that the control effect of interplanting the citrus with guava reaches 54-90%.

The invention discloses a diaphorina citri repellent. The active ingredients of the diaphorina citri repellent comprise one or more of beta-pinene, caryophyllene, benzoic acid, salicylate and thioether. The repellent provided by the invention is mainly originated from volatile components of plants, has the advantage of being natural and free of pollution, cannot cause pesticide residue and environmental damage, neither can cause death of natural enemies, even cannot generate drug resistance, and has a good application prospect at the aspect of preventing diaphorina citri.

The invention discloses a method for effectively preventing and treating diaphorina citri, and belongs to the field of plant protection. According to the method disclosed by the invention, an adhesiveis sprayed on the young shoots of murraya paniculata until the sticky feeling is obtained when the leaf surfaces is touched by a hand and diaphorina citri can be adhered; and the diaphorina citri adults which lay eggs or take food are adhered, cannot move or take food, and finally are dead, so that the effect of preventing and controlling the diaphorina citri is achieved. In the method disclosedby the invention, the rutaceae plant, murraya paniculata, is preferably used as a medium for preventing and treating the diaphorina citri; the murraya paniculata has the advantage of continuously growing young shoots for attracting the diaphorina citri to lay eggs; an element of glue spray is added, so that an effect of preventing and controlling the diaphorina citri is achieved. The method provided by the invention is green and environmentally friendly, and normal growth of plants is not influenced. Compared with an orchard spraying pesticide, the insecticidal effect is more concentrated andthe method is more environmentally friendly. Due to the fact that the processing time of the grown new shoots is not limited by trimming the murraya paniculata, the method can be realized all the yearround in south areas of the Yangtze River.

Provided are chimeric Hemiptera-active insecticidal toxin proteins comprising a Bt toxin peptide with activity against Hemiptera, a peptide multimer or fusion protein containing such peptide which binds to the gut of sap-sucking insects, preferably the Asian citrus psyllid, Diaphorina citri. This insect carries a bacterium associated with Huanlongbing disease or citrus greening and damages citrus crops. When bound, this peptide mediates the binding of the chimeric insecticidal protein to the target insect gut. Also described are coding sequences, vectors, and transgenic plants genetically modified to contain and express such insecticidal proteins. Other delivery mechanisms such as use of phloem-inhabiting bacteria, are also contemplated. Thus, the use of these toxins reduces economic loss due to feeding by the target insect and also reduces loss due to plant diseases spread by the target insect.

The invention discloses diaphorina citri specific molecular marker DNA (deoxyribonucleic acid) sequence and an identification primer and an identification method thereof. The diaphorina citri specific molecular marker DNA sequence is indicated as SEQ ID No. 1. The identification primer of the diaphorina citri specificity molecular marker DNA sequence is indicated as SEQ ID No. 2 and No. 3. The problems that traditional observation methods researching predators to predation effect of pests and biochemical methods researching relationships between predators and preys are complex, high in cost, lack of specificity of target and the like are solved, diaphorina citri specific molecular marker DNA sequence and the identification primer and the identification method thereof are provided, and an efficient method is provided for researching insect predation.

The invention provides a chitin binding protein Chitinase-like EN03 of diaphorina citri, and belongs to the technical field of pest control. The amino acid sequence of the chitin binding protein Chitinase-like EN03 of diaphorina citri is as shown in SEQ ID NO.1. The invention further discloses a preparation method of the chitin binding protein Chitinase-like EN03 of diaphorina citri. The coding gene of the chitin binding protein Chitinase-like EN03 of diaphorina citri is silenced through RNA interference, so that the diaphorina citri can die due to incomplete ecdysis or unsuccessful ecdysis, and the effect of preventing and treating diaphorina citri is achieved.

The invention provides a method for controlling Huanglongbing (HLB) disease of citrus plants through expressing genes encoding synthases for sesquiterpenes such as β-caryophyllene, and α-copaene, and combinations thereof, in citrus plants. Methods of controlling HLB comprising applying at least one purified sesquiterpene, which repels Diaphorina citri and/or Tryoza erytrae psyllid insects, so as to control the HLB disease of citrus plants, are also disclosed.