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331results about How to "Improve the effect of prevention and control" patented technology

Functional foliage fertilizer for preventing and controlling powdery mildew of tomatoes and cucumbers and application

The invention discloses a functional foliage fertilizer for preventing and controlling powdery mildew of tomatoes and cucumbers and application. The main raw materials for the foliar fertilizer include a solution of hydrolyzed starch Bacillus amyloliquefaciens YN2010-KC with CGMCC No.5722, other microbial bacterial solutions, a nitrogen source, a phosphorus source, a potassium source, trace element raw materials, humic acid and a stabilizer, and the functional foliage fertilizer is prepared through compounding in proportion. The foliar fertilizer is a novel ecological and safe fertilizer and can be used for foliar application and root irrigation of crops. The prevention and control effect on the powdery mildew of vegetables is high, the prevention and control effect on the powdery mildew of the tomatoes is up to 77.95-92.37%, the prevention and control effect on the powdery mildew of the cucumbers is up to 71.80-81.19%, the functional foliage fertilizer is non-toxic, residual-free, pollution-free and free of toxic and side effects, does not contain any harmful chemical substance, fertilizers can be decreased or neither fertilizer or pesticide is used, an entire ecological system is changed, and high-quality pollution-free agricultural products are produced out.
Owner:南宁汉和生物科技股份有限公司

Fluorescent quantitative PCR (polymerase chain reaction) primer for rapidly diagnosing nosema antheraeae and application of primer

The invention discloses a specific primer for diagnosing nosema antheraeae and an application of the primer. High-sensitivity specific detection for the nosema antheraeae is realized by an SYBR Green fluorescent quantitative PCR (polymerase chain reaction) method, the optimal reaction condition includes 95 DEG C and 30 seconds, circulation is performed for 40 times under the condition of 95 DEG C and 5 seconds and 62.5 DEG C and 30 seconds, and the melting curve condition includes 95 DEG C, 15 seconds, 60 DEG C, 60 seconds, 95 DEG C, 15 seconds, 60 DEG C and 15 seconds. Detection sensitivity is improved by two magnitude orders as compared with that of disclosed PCR technology, and lower detection limit includes that each reaction system contains 0.0046ng nosema genome DNA (deoxyribonucleic acid). Reliability of detection results is quantized by calculating primer amplification efficiency. The specific primer effectively solves the problems of low sensitivity, late detection period, easiness in leak detection, environmental pollution caused by conventional PCR nucleic acid coloring agents and the like, and has a wide application prospect in terms of antheraeae pebrine inspection and quarantine and cocoon quality improvement.
Owner:辽宁省农业科学院大连生物技术研究所
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