2211 results about "Germination" patented technology

The invention relates to the technical field of molecular genetic breeding, in particular to a KASP molecular marker related to salt tolerance in a mung bean germination period, a primer group, a kit and application. According to the KASP molecular marker Chr126769484 related to the salt tolerance in the mung bean germination period, primers are designed according to the KASP molecular marker, typing is carried out by utilizing specific matching of basic groups at the tail ends of the primers, the KASP molecular marker related to the salt tolerance in the mung bean germination period is developed, genetic typing is carried out, identification is accurate and convenient, the salt tolerance in the mung bean germination period can be predicted at low cost, and the application prospect is wide. The process of salt-tolerant variety breeding in the mung bean germination period is simplified, the breeding efficiency is improved, and the mung bean breeding process is accelerated.

The invention relates to the technical field of organic fertilizers and planting of watermelons and muskmelons, and provides a special fertilizer for milk irrigated watermelons and muskmelons and a double-stubble planting method for sweetness enhancement of milk irrigated watermelons and muskmelons. The special fertilizer for the milk irrigated watermelons and muskmelons comprises black goat manure, sophora alopecuroide, sesame seed meal, bran, organic fresh milk, brown sugar and zymophyte. The black goat manure is pulverized into powder by a pulverizer, the sophora alopecuroide is pulverizedinto 1 cm to 2 cm, and the ratio of the black goat manure, the sophora alopecuroide, the sesame seed meal, the brans, the organic fresh milk, the brown sugar and the zymophyte is 2700-3200: 80: 50: 150: 5: 1. The double-stubble planting method for sweetness enhancement of the milk irrigated watermelons and muskmelons comprises the following steps: selection of a nursery seedling plate and soil, cleaning and germination accelerating of seeds, soil preparation, transplanting, irrigation, topdressing, shaping and forking of watermelon and muskmelon vines and fruit bearing of the watermelons and the muskmelons. The problems that in an existing watermelon and muskmelon planting process, soil nutrients are damaged due to the fact that chemical fertilizers are used for a long time, and the sweetness of melons is reduced are solved.

The invention relates to a high-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings. The invention is characterized in that dormant axillary buds of semi-lignified young stems are used as the base. The method comprises the following main steps: sampling and disinfection treatment of explants, inducing of dormant axillary bud germination, inducing of adventitious bud and lateral bud high-frequency generation, strong seedling culture, test-tube rooting culture and acclimatization and transplanting. Finally, regenerative plants which have consistent parental character, excellent quality and strong stress resistance are obtained. According to the invention, lots of seedlings with consistent specification and excellent quality can be obtained within a short period of time, and marketization progress of Enkianthus chinensis is promoted powerfully.

The invention discloses talaromyces purpurogenum as well as a fermented supernatant and application thereof. The preservation number of the talaromyces purpureogenus F-4 is CCTCC NO: M 20252023, and the talaromyces purpureogenus F-4 has the growth promoting effects of dissolving phosphorus, fixing nitrogen and generating siderophores, also has the disease preventing and treating effects of preventing and treating garlic root rot, potato scab and wheat stem rot, and also can resist salt stress, alkali stress, salt-alkali composite stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the talaromyces purpureus F-4 can be used for inhibiting the growth of fusarium solani spores, streptomyces botrytis spores and fusarium pseudograminearum and the germination of the spores of the fusarium solani, the streptomyces botrytis and the fusarium pseudograminearum. The talaromyces purpurogenus and the fermented supernatant thereof can effectively control the growth and reproduction of plant pathogens, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, and reduce the risk of environmental pollution and agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention belongs to the field of biological agents, and particularly relates to bacillus siamensis and application thereof in the aspects of plant disease control, growth promoting and yield increasing. The bacillus siamensis XNC-3 provided by the invention is wide in antibacterial spectrum, can effectively inhibit various plant pathogenic fungi including phytophthora parasitica var nicotianae, has broad-spectrum antibacterial activity, and can be used for preventing and treating plant diseases, especially tobacco diseases. The bacillus siamensis has a promoting effect on tobacco seedling growth and seed germination, has growth-promoting and yield-increasing effects on tobacco, can remarkably increase the tobacco yield and improve the tobacco quality, is safe to the environment and has a very good application prospect.

Present invention teaches the method of using a keratin hydrolysis peptide (“KHP”) solution to promote the growth of soybean plants under flood stress. By selectively choosing specific weights of feathers and water, and treating the mixture to a high-temperature high-pressure hydrolysis process, the resulting solution is confirmed to contain at least 253 peptides and then apply to the soil containing the soybean at a specific growth stage after germination. Optionally, the KHP solution can be diluted by water as disclosed in the application.

The invention discloses a pseudo-ginseng seed storage method, relates to the technical field of seed treatment, and aims to solve the problems that pseudo-ginseng seeds are difficult to store, easy to lose vigor and severely mildew.The pseudo-ginseng seed storage method adopts a specially-made bionic microenvironment packaging unit to wrap the seeds, the inner layer of the specially-made bionic microenvironment packaging unit contains a moisture slow-release agent, and a bacteriostatic agent and active substances needed by germination can be intelligently released in a staged mode; the outer layer of the seed packaging box is a selective breathable film which integrates humidity and microbial contamination state indication functions and stress buffer characteristics, then the packaged seed is placed in an intelligent regulation and control storage cabin, and the temperature and humidity are accurately regulated and controlled by the storage cabin; gas components (such as low oxygen, high carbon dioxide or ozone) regulation and control, efficient air filtration and an intelligent monitoring and remote management system can be selected, the service life of the seeds can be remarkably prolonged, high germination rate and activity are kept, mildew is effectively inhibited, physiological after-ripening and germination are optimized, and intelligentization, standardization and refinement of seed storage management are achieved.

The utility model is suitable for the technical field of pepper germination accelerating, and provides a pepper seed germination accelerating device which comprises a germination accelerating box, the base is fixedly mounted in the germination accelerating box; the bearing disc is fixedly arranged on the base; the bearing disc is fixedly arranged on the base; the breathable net is fixedly mounted at the bottom of the bearing disc and is arranged corresponding to the base; the cloth liner is arranged on the bearing disc and is used for placing pepper seeds; the gauze is arranged in the germination accelerating box and is arranged corresponding to the bearing disc; the two traction mechanisms are both arranged on the germination accelerating box and are used for adjusting the position of gauze; the water replenishing mechanism is arranged on the germination accelerating box and is used for replenishing water to the pepper seeds. According to the pepper seed germination accelerating device provided by the scheme, the gauze can be turned over regularly, the germination accelerating effect is guaranteed, meanwhile, manual participation is reduced, and the workload is reduced.

The invention discloses a large-scale preparation method of ginseng exosomes, which comprises the following steps: culturing calluses: inoculating explants into an induction culture medium until ginseng calluses are generated; proliferation of the calluses: inoculating the newborn calluses into a proliferation culture medium for proliferation culture; inducing germination and rooting: after the ginseng callus is amplified, transplanting the ginseng callus into a germination induction culture medium until the ginseng callus is germinated, and then transferring the ginseng callus into a rooting culture medium for culturing and rooting; and preparing the exosome, namely grinding root hairs grown from the ginseng callus regeneration plant, uniformly mixing the ground root hairs with PBS (Phosphate Buffer Solution) or performing liquid culture on ginseng suspension cells, and finally performing centrifugal treatment to obtain the ginseng exosome. According to the large-scale preparation method of the ginseng exosome, the endotoxin pollution in the growth and development process is reduced through ginseng callus culture rooting, and then the ginseng exosome is prepared, so that the large-scale preparation requirement in modern production and processing is met.

The invention discloses a SbWRKY51 gene, a promoter and application of the SbWRKY51 gene and the promoter in improvement of salt tolerance of sorghum, and belongs to the technical field of plant genetic engineering. The SbWRKY51 gene is screened from sorghum, and the expression level of the SbWRKY51 gene is up-regulated after salt stress treatment. The protein coded by the gene belongs to a WRKY transcription factor family, has transcriptional activation activity, and can start the expression of a reporter gene. Sorghum overexpression strains and complementary strains of the gene are obtained, salt tolerance related physiological indexes are measured, overexpression of the gene can improve the seed germination rate, increase the seed root length, regulate ion balance and enhance the free radical scavenging capacity of plants, and the effect of the gene in the aspect of improving the salt tolerance is embodied. A further research finds that the SbWRKY51 gene can enhance the plant salt tolerance by regulating a lignin synthesis pathway. The technical scheme provides a basis for cultivating resistant plants, and has important guiding significance for enhancing the production potential of sorghum under a high-salt condition and promoting agricultural development.

Systems / methods for predicting the germination potential of seeds are disclosed. The methods include, by a processor: receiving an image comprising a plurality of seeds, and segmenting the image to identify instance masks associated with the plurality of seeds. For each of the plurality of instance masks, the methods include determining one or more of a plurality of characteristic labels, and determining based on the one or more of the plurality of characteristic labels, a germination potential of a seed associated with that instance mask.

The present invention relates to a strain of Glutamicibacter endophyticus subsp. halotolerans. The strain Glutamicibacter endophyticus NY3-1 of Glutamicibacter endophyticus subsp. halotolerans is deposited in the China Center for Type Culture Collection with the deposit number CCTCC No: M20242321. The 16S rDNA nucleotide sequence of the strain Glutamicibacter endophyticus NY3-1 is shown as SEQ ID NO: 1. This strain can metabolize normally in both salt-free and salt-containing environments, and can tolerate environments with a pH value of 6-11. It has the effects of improving the germination rate of plant seeds and promoting plant growth in both salt-containing and salt-free environments. Moreover, this strain can improve the soil environment of saline-alkali land, has broad application prospects, and provides a new biological resource for the biological improvement of saline-alkali land.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention discloses a sporosarcina sp. Strain RH34 and an application of the sporosarcina sp. Strain RH34 and the application of the sporosarcina sp. Strain RH34. The sporosarcina strain RH34 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33199. The sporosarcina strain RH34 can be used for preparing the sporosarcina strain RH34. The alfalfa rhizosphere microorganism sporosarcina strain RH34 is separated from alfalfa roots in salinized soil, and has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing biological membranes, producing siderophores, producing IAA and producing ACC deaminase. Seed soaking and germination under salt stress can obviously improve the germination index of alfalfa seeds. The growth condition of alfalfa seedlings can be improved under salt stress.

The invention belongs to the technical field of molecular markers, and discloses a molecular marker located in No.18 chromosome and related to the salt tolerance of soybean seeds in the germination period and application of the molecular marker. According to the present invention, the salt tolerance index of the soybean seed germination stage is determined, the stable and reliable molecular marker GmChr1856968562 closely linked with the salt tolerance ability of the seed germination stage is obtained through GWAS correlation analysis, the molecular marker is located at the 56968562 site on the No.18 chromosome of the soybean reference genome Glycine max Wm82. A2. V1, and the marker for KASP detection is developed based on the site, the invention also designs a primer group for amplifying the KASP marker. The invention further provides a method for rapidly identifying the salt tolerance of the soybean seeds in the germination period by using the primer group of the molecular marker, and the method is simple, convenient and rapid, accurate in identification result and good in popularization and application prospect.

The invention discloses scouring protection and seaweed restoration integrated ecological solidified soil and a preparation method thereof, and belongs to the technical field of ocean engineering and ecological restoration. The material is prepared from the following components in parts by weight: 800 to 1000 parts of sea mud, 200 to 400 parts of calcined coral sand, 50 to 150 parts of a modified chitosan gelling agent, 100 to 200 parts of a silicate geopolymer, 100 to 250 parts of slow-release nutritional ceramsite, 50 to 120 parts of seaweed seeds, 80 to 160 parts of a seawater activating agent and 20 to 50 parts of a microbial carrier loaded with saline-alkaline tolerant bacteria. The material has the characteristics of underwater dispersion resistance and self-leveling, can resist scouring at the flow speed of 2.5 m / s, and realizes that the 30-day accumulative germination rate of sea grass is greater than or equal to 80%. The method breaks through the bottleneck of function splitting of traditional material engineering protection and ecological restoration, synchronously completes scouring protection and seaweed community reconstruction, and is suitable for ocean engineering scouring protection and seaweed ecological restoration engineering.

The invention belongs to the technical field of biological control, and particularly relates to biological control fermentation liquor and a preparation method and application thereof, the preparation method comprises the following steps: S1, raw material treatment: selecting solidago canadensis as a plant raw material, cutting the solidago canadensis into pieces, performing autoclaved sterilization, adding water, and mixing to obtain a mixture; s2, adding the cultured bacillus subtilis and saccharomyces cerevisiae into the mixture in the step S1, mixing and inoculating, and stirring and fermenting to obtain a fermentation stock solution; s3, centrifuging the fermentation stock solution, taking supernate, and filtering to obtain the biological control fermentation liquor. The biological control fermentation liquor prepared by the invention can be used for controlling seed germination, seedling growth and flowering processes of solidago canadensis, and meanwhile, the biological control fermentation liquor can also be used for preparing products for controlling solidago canadensis. The problem that a biological medicament for preventing and treating solidago canadensis is lacked at present is solved.

The invention discloses application of a ZmFAH1 gene in regulating and controlling low temperature resistance of corn in a germination period, a related molecular marker and application of the molecular marker. The invention firstly provides the high-low-temperature-resistant ZmFAH1 gene, and the relative expression quantity of the high-low-temperature-resistant ZmFAH1 gene is obviously higher than that of a low-temperature sensitive material in embryos in a germination stage, buds in a bud stage, roots in the bud stage and roots in a seedling stage; the ZmFAH1 gene can significantly improve the low temperature resistance of corn in the germination period, and also can enhance the low temperature resistance of corn in the germination period and the seedling period. The invention further provides a molecular marker DNCAPS708 which is located at the 708 -710 sites of the CDS region of the negative chain ZmFAH1 gene of the first chromosome of the corn, and three deleted basic groups are GAA / <->. The research finds that the overexpression of the ZmFAH1 gene can effectively improve the low temperature resistance of the corn, which is of great significance to the molecular biology mechanism research of the low temperature resistance function of the corn.

The present invention relates to a polypeptide CYS3 that affects seed germination. The CYS3 polypeptide molecule affects the germination of immature tobacco seeds. Immature tobacco seeds picked 20 days in advance after pollination are used as test materials. The CYS3 polypeptide is expressed and then treated in the tobacco seeds. It is found that the CYS3 protein has a germination-promoting effect on the immature tobacco seeds, providing a practical technology for promoting tobacco seed germination and seedling emergence in production.

The invention relates to the technical field of biocontrol microbial agents, and provides a preparation method of trichoderma particles. Comprising the following steps: inoculating and culturing trichoderma in a PDB culture medium, centrifugally collecting trichoderma thalli, embedding the trichoderma by using sodium alginate powder as a wall material, carrying out microencapsulation treatment on the trichoderma to obtain a microencapsulated trichoderma agent, drying the microencapsulated trichoderma agent at low temperature, and mixing and granulating an organic matter base fertilizer and the microencapsulated trichoderma agent to obtain the trichoderma particles. The trichoderma is subjected to microencapsulation treatment, so that the release time can be delayed, the influence on the activity of the trichoderma in the germination process of the coated corn seeds is avoided, and meanwhile, the trichoderma can be slowly released in the subsequent corn growth process to achieve a continuous biocontrol effect; the trichoderma particles prepared by the method are high in stability, a relatively high viable count of trichoderma can be still kept within a relatively long storage time, the storage time of the trichoderma particles is greatly prolonged, and the actually required long-time storage requirement is met.

The invention discloses a rapid evaluation method for heat resistance phenotypes of non-heading Chinese cabbages. The method comprises the following steps: step 1, performing standardized seedling culture under the conditions of day and night constant temperature of 22 DEG C / 18 DEG C, relative humidity of 60%, illumination for 16h / darkness for 8h and light intensity of 20000Lux; step 2, extreme high temperature stress treatment: the temperature is 49 + / -0.5 DEG C, the humidity is 60%-70%, the illumination intensity is 20000Lux, treatment is performed for 8 hours, and then culture is recovered; step 3, observing phenotypic indexes including survival rate S, leaf damage index D, plant height inhibition rate H and new leaf germination number N; 4, constructing a comprehensive evaluation model, and calculating a heat resistance index HI; 5, plant heat resistance phenotype rapid evaluation. According to the method, variety heat resistance accurate classification is achieved, professional instruments are not needed, the single cycle only needs 20 days, the supply pressure of non-heading Chinese cabbages in the high-temperature season is remarkably relieved, breeding standardization of stress-resistant varieties is promoted, and agricultural sustainable development is promoted.

The invention belongs to the technical field of plant disease control, and particularly relates to bacillus agave, fermentation liquor, a biological agent and application. The invention discloses a strain of bacillus tequilensis, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus tequilensis is CGMCC NO.34830. The strain of bacillus tequilensis has the advantages that the preservation number is CGMCC NO.34830; the invention also discloses a fermentation broth and a biological agent prepared according to the bacillus agave, and the bacillus agave, the fermentation broth and the biological agent provided by the invention can effectively reduce the harm of root knot nematode disease to plants and promote seed germination or crop growth, and have the advantages of high efficiency, safety, environmental protection and difficult generation of resistance.

The invention discloses application of a rice OsbHLH083 gene in regulation and control of seed germination capability, and relates to the technical field of plant genetic engineering. The CDS sequence of the rice OsbHLH083 gene is as shown in SEQ ID NO. 2, and the amino acid sequence of the coded protein is as shown in SEQ ID NO. 3. The molecular biology function of the rice OsbHLH083 gene for regulating rice germination is studied, after the rice OsbHLH083 gene is edited through CRISPR / Cas9, compared with a wild type, the rice OsbHLH083 gene mutant rice seeds germinate slower, the rice OsbHLH083 gene and a protein coded by the rice OsbHLH083 gene can be used for regulating the phenotype of seed germination, and the rice OsbHLH083 gene mutant rice seeds germinate more slowly. And a gene resource is provided for the genetic improvement of rapid germination and seedling formation characters of the direct seeding rice seeds in the field.

Protecting open seed trenches from ingress of foreign material, such as plant residue. Preventing foreign material from entering the open seed trench during planting increases crop yield by minimizing delayed germination and late plant emergence. Various implementations of adjustable row cleaners may be used to prevent ingress of foreign material. Also, various shields may be disposed on the row units of a planter to prevent ingress of foreign material.

The invention discloses a rice root bud tracking method and system based on two-stage segmentation and topological feature fusion, and the method comprises the steps: firstly collecting a plurality of frames of seed images, then obtaining a seed individual through instance segmentation, obtaining a root, a bud and a main body of the seed individual through semantic segmentation, and then achieving the tracking of the seed main body through topological graph matching. The radical bud and the main body belong to the same individual in the image, so that the radical bud tracking is synchronously realized. According to the invention, automatic and accurate monitoring of the growth dynamic state of the root bud of the single seed in a dense scene is realized, and reliable technical support is provided for dynamic monitoring of the rice germination form.

The invention relates to a cavern environment-based tricholoma matsutake artificial cultivation method, and relates to the technical field of edible fungus cultivation, and the method comprises the following steps: mushroom stick preparation: mixing pine sawdust, corncob, bran, rice bran and an additive, adding water, bagging, sterilizing with high-pressure steam or normal-pressure steam, and inoculating tricholoma matsutake strains into each bag; environment construction: installing temperature and humidity regulation and light scattering equipment in cave depots, natural caves and air-raid shelters with the soil layer thickness of more than 50 m, the constant temperature of 10-20 DEG C and the humidity of 70-90%; in the hypha stage, the temperature is controlled to be 15-18 DEG C, the humidity is controlled to be 70-80%, and culture is conducted for 40-60 days; in the fruiting stage, the temperature is lowered to 12-16 DEG C in a stepped mode, the humidity is increased to 85-90%, and harvesting is conducted after fruiting bodies are induced to be formed. And recycling resources: crushing the waste mushroom sticks, and mixing the crushed waste mushroom sticks with soil according to a ratio of 1: 3 to prepare a fertilizer or mixing 10-30% of a new material for reuse. The produced tricholoma matsutake has the tricholoma matsutake alcohol content of 77.21 mg / kg and the polysaccharide content of 2.67%, the active components of the tricholoma matsutake are equal to those of wild tricholoma matsutake, the hypha germination rate is greater than or equal to 95%, the growth period is shortened to 40-60 days, and the raw material consumption is reduced by

The invention belongs to the technical field of plant molecular markers, and particularly discloses an Indel molecular marker related to the salt tolerance of brassica napus and application of the Indel molecular marker. The marker is located at the position of an A01 chromosome scafoldA0133077947 of a brassica napus reference genome ZS11.v10, and the genotype of a polymorphic site is CTAA or C. The invention further discloses a method for identifying the marker of the brassica napus. Large-scale group phenotypic association analysis proves that the molecular marker is remarkably associated with the salt tolerance of the brassica napus in the germination period, and when the polymorphic site genotype is CTAA, the salt tolerance in the germination period is high; when the genotype of the polymorphic site is C, low salt tolerance in the germination period is shown. The invention finds that the polymorphic site is obviously associated with the salt tolerance of the brassica napus for the first time, provides a powerful molecular tool for solving the problems of difficulty in phenotype identification, long period and low efficiency in salt tolerance breeding of the brassica napus, can accurately screen salt-tolerant materials in the early stage, and greatly accelerates the breeding process of new varieties of the salt-tolerant brassica napus.

The invention provides a salt-tolerant gene, namely a PrABC1 gene and application thereof, and also provides a vector containing the gene, an expression cassette, a recombinant cell, a transgenic plant and a method for producing the transgenic plant. Specifically, the gene has a nucleotide sequence as shown in SEQ ID NO: 1 or SEQ ID NO: 2. The plant growth regulator can be used for improving the tolerance of plants to a salt stress environment, relieving the adverse effects of a high-salt environment on the germination rate and root length of the plants, reducing the water loss rate of the plants under the high-salt stress condition and reducing the reduction of the chlorophyll content.