13672 results about "Germination" patented technology

The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. In one embodiment, a high-throughput, non-destructive method for analyzing individual seeds in a population of seeds comprises removing a sample from a plurality of seeds in the population while preserving the germination viability of the seed and analyzing the sample for the presence or absence of one or more characteristics of at least one genetic or chemical trait.

The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. In one embodiment, a high-throughput, non-destructive method for analyzing individual seeds in a population of seeds comprises removing a sample from a plurality of seeds in the population while preserving the germination viability of the seed and analyzing the sample for the presence or absence of one or more characteristics of at least one genetic or chemical trait.

The present invention relates to a method for improving plant productivity, and in particular crop yields, via the introduction of an endophytic microorganism to the subject plant. More particularly, the present invention is directed to a method for improving cereal crop productivity via the introduction of an endophytic actinomycete to the subject crop. The method of the present invention facilitates the improvement of crop productivity, such as increasing germination, by, inter alia, providing the subject plant with disease bio-control capabilities and up-regulating plant growth promoting activities. The present invention is also directed to novel endophytic microorganisms and uses thereof.

The invention provides a new crop seedling growing matrix, wherein the pH value of the seedling growing matrix is 6.0-6.5. The seedling growing matrix comprises the components by the weight percentage: 50%-80% of an inorganic matrix, and 15-30% of an organic matrix, wherein the organic matrix includes a composition of peat, fermented cattle manure, fermented pig manure, plant ash and an additional organic matrix, and the inorganic matrix includes a composition of vermiculite, perlite and an additional inorganic matrix and is composed of inorganic matrix particles with different particle sizes. The seedling growing matrix provided by the invention has relatively high total porosity and maximum water holding capacity, and is higher in aeration porosity ratio, more reasonable in water-gas ratio, small in bulk density and convenient to transport and use. The seedling growing matrix provided by the invention has the advantages of wide range and many types of applicable crops, high germination rate, less morbidity and good seedling growth vigor.

The invention discloses a method for cultivating phellinus igniarius. The method includes the steps that firstly, short-cut wood is directly placed in a material bag or placed in the material bag after being bundled, the two end faces of the short-cut wood or the two ends of a short-cut wood bundle are paved with cultivation media, an opening of the bag is tightened, and then a material package is acquired; secondly, the material package is sterilized, cooled and then inoculated, and then phellinus igniarius is acquired through cultivation, wherein the cultivation media are radix puerariae residues. The radix puerariae residues are used as the cultivation media, so that growth and reproduction of phellinus igniarius mycelia can be stimulated, the mycelia are stronger, germination and field planting of the phellinus igniarius mycelia are accelerated, and eventually the spawn running time can be shortened by about 15 d; in addition, invasion and infection of contaminating microorganisms can be reduced, and statistics show that the contamination rate of the material package can be reduced by 11.7% to 17.9% and the yield of phellinus igniarius can be increased by more than 15%.

The invention provides a method for quickly reproducing bletilla striata seedlings, which comprises the following steps: 1) using mature seeds of bletilla striata as explants; 2) sterilizing the seeds and inoculating the seeds to a germination culture medium to allow the seeds to germinate in about 7 days, cutting protocorms formed in about 14 days, and transferring the cut protocorms onto a protocorm proliferation culture medium; 3) transferring the protocorms onto a cluster bud induction and proliferation culture medium; and 4) cutting the cluster buds, allowing the cluster buds to propagate massively, transplanting 3-centimeter seedlings grown from the cluster buds onto a rooting and seedling strengthening culture medium, and transplanting the seedlings when the seedlings grow to 7 to 10 centimeter. The method solves the problems of virus carrying seedlings, deterioration of variety, low effective yield and the like of the conventional bletilla striata asexual reproduction. When the method is used, a large number of asexual reproduction virus-free seedlings can be obtained under a manual control condition; compared with the conventional reproduction method, the yield is improved by 30 percent; and thus, the method has an active significance for reliving market contradiction between supply and demand for bletilla striata.

The invention discloses a breeding method for aseptic seeding and test tube seedling formation of orchids and the adopted broad-spectrum culture medium. In this method, a healthy orchid mother plant is selected, and artificially pollinated when it blooms, and the fruit that develops to maturity after pollination and has not cracked is used as an explant, or the orchid fruit that is basically mature and not cracked is taken from the wild as an explant. Implants undergo reproductive steps such as aseptic sowing, seed germination, strong seedling cultivation, and test-tube seedling transplantation. The specially prepared medium is used for aseptic sowing and seed germination to obtain small plants, and then the small plants are cultivated on the specially prepared strong seedling medium, and the seedlings are hardened under natural light in the greenhouse for 7-14 hours before being released from the bottle. One day, the test-tube seedlings were taken out, the root medium was washed off, and they were planted in a mixed substrate of bark, bluestone and peat, and further cultivated into seedlings. The invention has the characteristics of high seed germination rate, fast seedling growth, good seedling quality, low cost, etc., can obtain a large number of test-tube seedlings in a short period of time, and the transplanting survival rate of the seedlings can be kept above 90%. An effective way can be provided for the production of orchid seedlings.

The invention relates to a method for accurate fertilization for apple tree. The method decides target yield according to soil fertility based on 4000 kg of high quality organic fertilizer, then determine fertilizer consumption according needed fertilizer, fertilizer supply ability by soil and fertilizer availability, dressing all organic fertilizer, 70% of the nitrogen fertilizer and phosphatic fertilizer and 50% of potassium fertilizer as base fertilizer, taking left fertilizer for additional dressing, dressing the left 30% nitrogen before germination, dressing the left 30% phosphatic fertilizer before flower bud differentiation, dressing left potassium fertilizer during fruit expanding period, spraying fertilizer on leaf in different growing period for 6- 8 times in one year. The productivity and output value are increased by 21.5% and 21.3% respectively, the high- quality fruit rate is increased from 35% to 62%,and the net value is added by 615 yuan per mu.

The present invention is directed to improved seed coatings which facilitate fall or early spring planting while maintaining seed dormancy until soil temperatures are appropriate for successful germination. The improved seed coatings contain encapsulated phase change materials within a polymeric shell which preserve the dormancy of the seed during early planting by slowing the rate at which the seed temperature rises in the event of a temperature spike thus preventing premature germination. The encapsulated phase change material is a material characterized by a solid / liquid or liquid / solid phase change which occurs at a temperature which ranges from about −5 to about 20° C., preferably between about 0 to about 19° C., most preferably between about 5 to about 15° C. The solid / liquid or liquid / solid phase change is further characterized by an effective enthalpy of fusion / crystallization for the solid-liquid / liquid-solid phase change equal to or greater than 20 J / g when determined by Differential Scanning Calorimetry.

Provided is a bletilla striata rapid propagation seedling cultivation method. Fermented bark and a mixture of humus and river sand serve as a base material, the base material is laid on a seedling cultivation vessel, 1-2 layers of gauze is laid on the base material, water is poured to soak the base material, bletilla striata seeds are sown on the gauze, a transparent plastic film is utilized to wrap the seedling cultivation vessel, and the seedling cultivation vessel is placed in an artificial intelligent cultivation box to grow seedlings and strengthen the seedlings. The bletilla striata rapid propagation seedling cultivation method breaks through a traditional corm planting and cultivation mode of bletilla striata, the quality degeneration problem existing in vegetative propagation is resolved, and a new path is created for sexual propagation seedling cultivation of the bletilla striata genus of orchidaceae. The bletilla striata rapid propagation seedling cultivation method is simple in operation, low in cost and short in seedling cultivation period; the germination rate reaches up to 90%, the planting percent is 70%-80%, and good practical popularization significance is provided for large-scale propagation seedling cultivation of the bletilla striata.

Polynucleotides and polypeptides incorporated into expression vectors have been introduced into plants and were ectopically expressed. The polypeptides of the invention have been shown to confer at least one regulatory activity and confer increased yield, greater height, greater early season growth, greater canopy coverage, greater stem diameter, greater late season vigor, increased secondary rooting, more rapid germination, greater cold tolerance, greater tolerance to water deprivation, reduced stomatal conductance, altered C / N sensing, increased low nitrogen tolerance, increased low phosphorus tolerance, or increased tolerance to hyperosmotic stress as compared to the control plant as compared to a control plant.

The invention belongs to the technology field of grain processing, specifically a method for producing active germinated brown rice and products thereof. The method comprises the following steps: screening brown rice raw material to remove impurity, washing, sterilizing, soaking in a nutrient fluid supplemented with calcium lactate, gibberellin, phytase, cellulase and vitamin C for 10-36 hours at 10-40 DEG C, draining, incubating for 10-36 hours at 10-40 DEG C under relative humidity of 50-90% to allow germination, drying with hot air of 40-55 DEG C for 0.5-3 hours until the sprout grows about 0.5-1.2 mm in height, drying with 2-10 w / g microwave for 2-10 minutes until the water content of germinated brown rice is below 15%. Compared with the prior art, the germinated brown rice is nutritious, retains almost all nutrients of brown rice and has high nutritional value.

A high-throughput method is provided for analyzing individual seeds in a population of seeds. The method generally includes removing a tissue sample from at least one or more seeds in the population of seeds while preserving germination viability of the at least one or more seeds from which the tissue sample is taken, depositing the tissue sample into an individual compartment of a sample tray, contacting the tissue sample with an extraction buffer to remove DNA from the sample, and analyzing the extracted DNA for the presence or absence of a transgene of interest. The extraction buffer may be present in the individual compartment of the sample tray before the tissue sample is deposited into the individual compartment.

The invention discloses a method for growing watermelons. The method includes the steps of processing seeds and accelerating germination, sowing and raising seedlings, applying base fertilizer on the whole land, transplanting the seedlings, performing field management, harvesting and the like. The method for growing watermelons is easy to operate, convenient to manage, good in growth vigor of watermelons, high in disease resistance, uniform in result nature and high in yield, the yield per mu can reach 4000 kg to 5000 kg, the watermelons are large, good in quality, high in sugar content, bright in flesh color, delicate in flesh and high in water content and commodity value, and therefore the method has great significance for increasing income of melon farmers.

The invention discloses a drought-resistant energy crop seed pelleting agent, a preparation method and a using method thereof. The seed pelleting agent comprises the following components: straw powder or overrotten organic matter, a plant growth regulating substance, a water absorbent resin, a filler, an adhesive, an insecticide, a bactericide, urea and monopotassium phosphate. The method for seed pelleting comprises the following steps of: 1) spraying an adhesive water solution on the surfaces of drought-resistant energy seeds evenly; 2) mixing the drought-resistant energy seeds of which the surfaces are the adhesive water solution and a pelleting agent for coating; and 3) repeating the step 1) and the step 2) until the diameters of the seeds meet the requirement. The invention breaks the traditional idea that the pelleting agent absorbs water in soil of a plough layer, establishes a theory of absorbing water before the sowing and sowing after the germination acceleration, and realizes the aim that the pelleting agent can absorb the water before the sowing after the coating, does not disperse after the water absorption, has good expansibility, ensures that the seeds germinate in the palleting agent and can adopt mechanized sowing by adding crop straw powder or the overrotten organic matter which is rich in water absorption expansibility into the components of the pelleting agent.

The present invention provides compositions and methods for resolving bioavailable oxygen supply to plants subjected to hypoxic stresses. Compositions of the invention comprise an oxidizing agent, wherein the level and rate of oxygen released from the composition is controlled. Use of the compositions of the invention address hypoxic stress and also stimulate plant growth, enhance plant vigor, and / or improve crop yield.

The invention discloses a mechanically-mulching cultivation method of organic rice, relating to a rice cultivation method. The purpose of the method provided by the invention is to solve the weeding problem in paddy fields in the existing cultivation method of organic rice. The method comprises the steps of 1, selecting field environment, namely (1) selecting soil, (2) selecting air quality and (3) selecting a water source; 2, performing soil preparation in the field, namely (1) ploughing, (2) fertilizing and (3) digging canals and harrowing; 3, preparing seedbed and bed soil, namely (1) preparing seeding environment, (2) preparing seedbed and (3) preparing bed soil; 4, germination treatment of seeds, namely (1) drying seeds, (2) performing plasma treatment, (3) soaking seeds and (4) accelerating germination; 5, sowing; 6, managing seedlings, namely (1) performing insect prevention treatment, (2) managing temperature, (3) managing water, and (4) fertilizing; 7, mulching, transplantingseedlings and managing the field, namely (1) making a bed and mulching, (2) transplanting, (3) irrigating, (4) dressing foliar fertilizer, (5) managing water layers in the field, (6) weeding and (7) preventing insects. The method is mainly used for cultivating organic rice by mechanical mulching.

Agricultural planter row units feature soil finishing assemblies for closing a seed groove after seed is placed in the soil. An adjustable furrow closing assembly enhances upper seed groove coverage and closure with soil resulting in sustained relative humidity levels and optimum seed-to-soil contact for faster seed germination. The furrow closing assembly includes a closing wheel assembly having at least one closing wheel and a press wheel assembly having a press wheel following behind the closing wheels. The closing wheels and press wheel are attached to the planter row unit in a manner allowing the press wheel to move vertically relative to the closing wheels. Adjustable down-force systems are provided to vary the down force applied to the closing wheels and the press wheel to maintain optimum soil contact in irregular terrain and in varying soil densities and conditions to provide optimum soil coverage and compaction of the seed bed.

The present invention relates to eco-friendly compositions and methods for providing plant growth enhancing formulations comprising mixtures of microbial isolates. The microbial consortium is developed for customized solution of soil health related problem such as with plant growth promoting properties including root and shoot length elongation, early and high germination rate, high yield, decrease in soil pathogenic load and increase soil micro and macronutrient status. These specifically designed polymicrobial formulations would further provide protection against plant pathogens lowering the need for nitrogen containing fertilizers, solubilize minerals, protect plants against pathogens, and make available to the plant valuable nutrients, such as phosphate, thus reducing and eliminating the need for using chemical fertilizers and chemical pesticides.

The invention discloses a construction method for spraying-seeding kind-loam matrix on a side slope. The construction method comprises steps of A) analyzing stability of the side slope and reinforcing the side slope, B) eliminating dangers for the side slope, C) hanging a net for a slope surface of the side slope and constructing anchor rods, D) arranging a river system for the side slope and arranging a drainage system on the side slope, E) spraying and seeding seeds on the side slope via a kind-loam matrix spraying-seeding way, and F) conducting side slope maintenance management work including covering a non-woven fabric, watering, applying fertilizer, preventing diseases and insects, supplementing seeds and post-monitoring. Two-layered spraying-seeding way is applied; through bionic operation, kind-loam matrix in the nature suitable for the side slope can be simulated; plant seed germination, root development, microorganism activity and nutrient conversion can be facilitated; plant growth development can be promoted; and great application prospect can be achieved.

A floatable growth tray for the germination of seedlings has a plurality of cells adapted to receive a mixture of a growth median and seeds. The tray has sealed pockets forming air chambers to enable the tray to float. The cells have holes forming openings extending through a bottom surface of the tray permitting water to enter the cells to promote germination of the seeds.

A method and apparatus for increased yields of water-soluble polyphenols from plant seeds is provided. The method includes inducing seed germination within a selected range of temperatures. The method also includes inducing germination in an atmosphere having elevated carbon oxygen concentration and / or a reduced concentration of oxygen. The method further includes exposing the seeds to light and extracting water-soluble polyphenols with an aqueous solution without adding an organic solvent such as alcohol.

The invention relates to a plant seedling raising technique, in particular to a camellia oleifera container seedling raising technique which aims to solve the problems of low seedling survival rate and low seedling raising efficiency of a camellia oleifera seedling raising technique in the prior art. The camellia oleifera container seedling raising technique includes the steps of (1) collecting seeds, and placing the collected seeds in a refrigeratory for cold storage and reserve at the temperature of 0-5 DEG C; (2) pre-treating the seeds, and soaking the seeds prior to sand storage for accelerating germination; (3) sowing, sowing the camellia oleifera seeds subjected to germination accelerating in a container containing seedling raising substrates in a spot manner, and then placing the container on a seedbed; (4) grating rootstocks of seedlings; (5) performing grafted seedling management including water management, rootstock removal and weeding management, light and temperature management, nutrient management and the like; (6) performing biennial seedling production management; and (7) performing outplanting management. The technique has the advantages of high seedling survival rate, high breeding efficiency and good reliability; and seedling survival rate of the seedling raising technique is 10-20% higher than that of inarching seedling raising and leaf cutting seedling raising techniques.

The invention discloses a method for rapidly growing and cultivating dendrobium officinale, which comprises the following steps of: taking back mature shadow fruit seeds in the country of origin for wild dendrobium officinale, and placing the seeds in a clean culture dish; after natural crack, pouring the seeds into a clean beaker, sealing the clean beaker, and storing the beaker in a low temperature box; performing a certain sterilization procedure on the stored seeds, placing the seeds in an MS minimal medium, performing seed germination cultivation, wherein the seeds gradually become green and form protocorm, and observing that needles appear in the protocorm by using magnifying glass; transferring the protocorm into the MS minimal medium, performing protocorm proliferation and differentiation culture, observing that plantlets are differentiated from the protocorm in a bottle along the production of partial new protocorm; and transferring the plantlets into the MS minimal medium, and performing strong seedling and rooting culture, seedling hardening and transplanting. The survival rate is over 97 percent without the seedling hardening process, and the yield is doubled. The growth speed is improved by 50 percent, the content of each component is equivalent to that of the wild dendrobium officinale, and the contents of amino acid and microelement are higher than those of the wild dendrobium officinale.

The invention discloses an imitative wild sexual reproduction and breeding technique of gastrodia tuber. Main steps comprises that breeding is carried out to get mature gastrodia tuber seeds and good sprouting strain, mature class III shoestring fungus is adopted as production seed, which is planted under a natural hillside forest that is with over 80 percent of shade density and in which wild gastrodia tuber grows and is distributed; artificial mixed germination strain is adopted; room temperature is controlled so as to produce germination strain with seed; sexual reproduction, breeding and planting time is selected between June and August; the breeding technique is also suitable for field breeding. The invention has the advantages that: 1. bad factors resistance property of sexual reproduction and breeding of gastrodia tuber is improved. 2. Gastrodia tuber seeds are germinated to lead the growth cycle of protocorms to be a time slice that is basically matched with an animated period of the shoestring fungus; inoculation rate and survival rate of the protocorms are increased. 3. Raw materials are obtained locally, thus reducing cost; sustainable development of non-pollution gastrodia tuber planting industry is promoted in ecological agriculture forests. 4. The small site breeding method enhances competitor infection resistance of the gastrodia tuber seed.

The invention discloses a method for the in-situ dynamic observation and the measurement of the root system of a plant, which comprises the following steps: designing a transparent cultivation container and a growth medium; sterilizing seeds, accelerating germination, moving seedlings to the transparent cultivation container after budding, and keeping the natural growth of the root system of the plant in the transparent growth medium; scanning the root system by a three-dimensional laser scanning instrument, carrying out the in-situ observation and the image acquisition of the three-dimensional configuration of the root system and completing the steps of image collection, reconstruction, simulation and the like. By the three-dimensional visualization analysis of the in-situ growth condition of the root system of the plant, the invention directly reflects the three-dimensional growth condition of the root system, does not need destructive detection and realizes dynamic observation.

There is provided a packaging container which reserves an acidic beverage having a relatively high acidity and allows spore to remain properly without corrupting and the acidic beverage can be aseptically filled and reserved at a normal temperature with low cost without utilizing a container having high heat-proof property and expensive manufacturing equipment. An interior of a container 2 is sterilized by a sterilizing agent “b” and a heated water “c” so as to allow bacterial spore to be alive but not to allow bacteria vegetative cell, mold and yeast to be alive, and a sterilized content “a” having an acidity of an extent of suppressing germination of the bacteria spore fills the container at a normal temperature or low temperature, and then the container is sealed by a lid 3, thereby providing a packaging container 1.