223 results about "Germination" patented technology

The invention relates to the technical field of organic fertilizers and planting of watermelons and muskmelons, and provides a special fertilizer for milk irrigated watermelons and muskmelons and a double-stubble planting method for sweetness enhancement of milk irrigated watermelons and muskmelons. The special fertilizer for the milk irrigated watermelons and muskmelons comprises black goat manure, sophora alopecuroide, sesame seed meal, bran, organic fresh milk, brown sugar and zymophyte. The black goat manure is pulverized into powder by a pulverizer, the sophora alopecuroide is pulverizedinto 1 cm to 2 cm, and the ratio of the black goat manure, the sophora alopecuroide, the sesame seed meal, the brans, the organic fresh milk, the brown sugar and the zymophyte is 2700-3200: 80: 50: 150: 5: 1. The double-stubble planting method for sweetness enhancement of the milk irrigated watermelons and muskmelons comprises the following steps: selection of a nursery seedling plate and soil, cleaning and germination accelerating of seeds, soil preparation, transplanting, irrigation, topdressing, shaping and forking of watermelon and muskmelon vines and fruit bearing of the watermelons and the muskmelons. The problems that in an existing watermelon and muskmelon planting process, soil nutrients are damaged due to the fact that chemical fertilizers are used for a long time, and the sweetness of melons is reduced are solved.

The invention relates to a high-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings. The invention is characterized in that dormant axillary buds of semi-lignified young stems are used as the base. The method comprises the following main steps: sampling and disinfection treatment of explants, inducing of dormant axillary bud germination, inducing of adventitious bud and lateral bud high-frequency generation, strong seedling culture, test-tube rooting culture and acclimatization and transplanting. Finally, regenerative plants which have consistent parental character, excellent quality and strong stress resistance are obtained. According to the invention, lots of seedlings with consistent specification and excellent quality can be obtained within a short period of time, and marketization progress of Enkianthus chinensis is promoted powerfully.

The application provides a peanut germination period cold tolerance related KASP molecular marker, a primer group and an application thereof, and belongs to the technical field of molecular markers.The RIL population constructed by using the cold tolerance variety Sihelianhong and the low temperature sensitive variety Jinnonghei No.3 as materials, a SNP site closely related to the germination period cold tolerance is developed at the Chr.18:2374669 site of the peanut 18th chromosome, and the site has A / C single nucleotide polymorphism.The application also designs a primer group for detecting the SNP site, and the primer group comprises a first upstream primer, a second upstream primer and a universal downstream primer.The primer group can quickly identify the peanut germination period cold tolerance, has the advantages of accuracy, rapidness, low cost, short identification period, simple operation and the like, and provides an efficient molecular detection tool for the peanut planting industry in high latitude and low temperature areas.

This utility model discloses a constant temperature and humidity device for germinating larch seeds, relating to the field of seedling equipment technology. The utility model includes an insulated shell, with a storage mechanism fixedly connected to its inner cavity. A humidity-regulating component is fixedly connected to one side of the insulated shell, and a ventilation component is connected to the top of the insulated shell. The storage mechanism includes an inner shell disposed within the inner cavity of the insulated shell. This utility model, through the cooperation of the inner shell and the insulated shell, forms a double-layered insulated space, effectively reducing heat loss and ensuring stable internal temperature. The connecting plate, bracket, and tray design within the inner shell facilitates layered seed placement, not only improving space utilization but also ensuring good air circulation for each layer of seeds. A heater rapidly raises the internal temperature, and a lighting lamp simulates natural light conditions, providing suitable illumination for seed germination and helping to improve germination rate and quality.

This invention relates to the field of no-till seeding devices, and more particularly to a general-purpose no-till seeding device for wheat and rapeseed, comprising: a frame, rollers, a double-disc stubble-breaking blade, a seeding mechanism, and a covering roller; a storage bin is provided on the frame; the rollers are rotatably connected to the frame; an installation block is fixedly connected to the frame; the double-disc stubble-breaking blade is rotatably connected to the installation block; a motor for driving the double-disc stubble-breaking blade is provided inside the installation block; a support rod is fixedly connected to the installation block; in this invention, soil is moved to the crushing roller on a vibrating screen by a suction air pump, crushed into small soil particles, and then screened. The small soil particles fall into the furrow through a transfer funnel and a covering pipe to complete the covering. Subsequently, the covering roller lightly presses the soil to complete no-till seeding. Covering the seeds with small soil particles prevents seed hypoxia and soil compaction, thus avoiding restrictions on seed growth and germination.

This application belongs to the fields of microbiology and cell biology, and provides a method for directly preparing germination-promoting fungal protoplasts from orchid protocorms, comprising: (1) physical homogenization; (2) filtration; (3) removal of plant contents and residual cell walls; (4) purification of mycelial clusters; and (5) release and purification of fungal protoplasts. Through the rational design of homogenization and purification steps, this application achieves high-yield, high-purity, and high-vitality preparation of germination-promoting fungal protoplasts, providing excellent materials for orchid gene variety breeding and genetic engineering operations.

The present disclosure relates to a composition for coating of organically grown plant seeds and its use for improving the germination of plant seeds. The present disclosure further relates to a seed coating for a plant seed comprising a composition and a method for coating of plant seeds and for improved seed germination of organically grown plant seeds.

This invention discloses a *Armillaria mellea* contact induction device and control system for improving the inoculation rate after *Gastrodia elata* seed germination. It relates to the field of co-cultivation technology of edible and medicinal fungi and medicinal plants, and includes a shell, a germination support module, a mycelial induction module, a contact bridge module, a nutrient pulse module, an environmental modulation module, a dual-evidence detection module, and a main control module. The main control module generates an inoculation window index and, after trial contact, switches between a maintained contact state and a withdrawal state based on the recovery curve to complete the contact induction and colonization maintenance of *Armillaria mellea* with *Gastrodia elata* protocorms.

The application discloses application of HDP4 gene in regulating seed germination under adversity stress. The HDP4 gene can be used for regulating seed germination rate under adversity stress, and a HDP4 gene knockout strain shows a phenotype of fast seed germination in a high-salt and drought environment. The application can be used for solving the problem of low seed germination rate in the high-salt and drought environment, and has a wide application prospect.

The application discloses a wild hundred-petal grass domestication cultivation method and particularly relates to the field of wild plant cultivation technology, and comprises the following steps: step one, wild resource investigation and conservation, resource investigation is conducted on wild populations of hundred-petal grass, accompanying plants, original habitat types, soil conditions, illumination conditions, humidity conditions and distribution characteristics are recorded, germplasm resources of different populations are synchronously collected, including seeds and rhizomes, and the collected samples are numbered, stored in different zones and registered in traits, a seedling test area and a germplasm resource basic file are established, and basis is provided for subsequent directional domestication. The application improves seed germination rate and seedling uniformity, also improves transplanting survival rate and field adaptability, realizes wild resource protection and sustainable utilization, constructs an ecological planting mode suitable for local climate, improves stability and controllability of medicinal material quality, reduces chemical input and meets green development requirements.

The present application relates to the field of biotechnology, and provides a strain capable of accelerating and promoting seed germination of old crow petal and application thereof, the strain is isolated from soil of old crow petal seedling, and is identified as Aspergillus niger through ITS sequence sequencing after purification, and the taxonomic name is Aspergillus niger, and the preservation number is CCTCC NO: M 2025709. Through the concomitant germination test of the strain and old crow petal plant seeds, the results show that the strain can obviously accelerate the seed germination of old crow petal, and improve the seed germination rate.

The application discloses a kind of vegetation repair methods of high-cold region soil and stone slope, solve the technical problem that the prior art construction process is complex, vegetation survival rate is low.It includes: preparation of binder and the binder is evenly sprayed on the soil and stone slope needing protection using mud spraying machine;On the soil and stone slope that spraying slope-polylactic acid three-dimensional net binder is finished;Laying soil nutrient layer;Mix the seeds of elytrigia repens, thalictrum, pot marigold, groundsel, purple-flowered needle grass and arrow leaf ligusticum, the mixed seed after processing, soil and water are fully stirred and mixed to form grass seed mud slurry, and the grass seed mud slurry is evenly sprayed on the soil nutrient layer;After seed germination, drill expansion bolt hole, chemical bolt is placed in the hole of expansion bolt, and expansion screw is drilled and fixed using.The application shows remarkable effect in improving vegetation survival rate, enhancing slope stability, simplifying construction process and reducing cost and improving biodiversity.

The application discloses a tissue culture and rapid propagation method for Yangmei of Alocasia macrorrhiza and a special combined culture medium thereof, and the method comprises the steps of sterilizing an explant, carrying out primary culture and subculture and proliferation culture in stages, wherein the primary culture is performed by first inoculating a stem segment into an induction culture medium containing 6-BA 0.8-1.2 mg / L, IBA 0.05-0.15 mg / L and PVP-40 to induce germination, and then transferring the stem segment into an elongation culture medium containing 6-BA 0.1-0.5 mg / L, NAA 0.01-0.05 mg / L and PVP-40 to promote elongation; the new shoots are then transferred into a subculture medium containing 6-BA 1.0-1.8 mg / L, NAA 0.1-0.2 mg / L, GA3 0.5-1.5 mg / L and PVP-40 to proliferate. The application effectively solves the technical problems that Yangmei is prone to brown and death, and it is difficult to simultaneously achieve budding and high growth by means of two-stage hormone stepwise regulation and cooperation with a brown prevention agent, and realizes efficient and large-scale breeding of high-quality seedlings.

The application belongs to the field of in vitro rapid propagation of Chinese medicine seedling, and particularly relates to a high-efficiency liquid suspension culture method of pinellia ternate, which comprises obtaining of pinellia ternate explants under sterile conditions, induction of embryogenic callus, liquid suspension culture, somatic embryo development and maturation, somatic embryo germination and transplanting of regenerated seedlings. By using the method, the induction rate of pinellia ternate embryogenic callus can reach 81.6%, stable mature cotyledon embryos can be obtained, and the embryos can be regenerated into complete plants, a high-efficiency and stable regeneration system is successfully established, favorable conditions are created for industrial production of pinellia ternate fine seedlings, and a good foundation is laid for genetic transformation of pinellia ternate.

This invention proposes a method for improving the germination characteristics of wild soybean seeds, belonging to the field of seed germination treatment technology. The method includes the following steps: pre-cooling and rewarming treatment of wild soybean seeds. The pre-cooling treatment involves storing the wild soybean seeds at -2℃ to 2℃ for 6 to 8 days to obtain pre-cooled seeds. The rewarming treatment involves rewarming the pre-cooled seeds at 10℃ to 30℃ for at least 7 days to obtain pre-cooled and rewarmed seeds. The pre-cooled and rewarmed seeds are then subjected to a first contact treatment with 98% concentrated sulfuric acid for 10 to 50 minutes. After rinsing with water, dormant seeds are obtained. This method can improve the germination rate, germination potential, germination index, and vigor index of wild soybean seeds, and is simple to operate and easy to promote and apply.

The application provides a method for improving the germination rate and storage capacity of kiwi pollen, and belongs to the technical field of forest tree breeding. The method comprises the following steps: S1, placing the pollen of male kiwi flowers in 24-28 DEG C light treatment for 10-14 hours; S2, spreading the pollen treated in S1 on an in vitro germination medium for light culture; S3, observing the germination state of the pollen and calculating the survival rate of the pollen. According to the method, the pollen is first placed in 24-28 DEG C light pretreatment for 10-14 hours, so that the germination rate of the pollen is significantly higher than that of fresh pollen, and compared with the pollen without pretreatment, the method can further improve the pollen storage capacity of kiwi pollen under 4 DEG C and-20 DEG C storage conditions, and the decrease range of the pollen germination rate during storage is significantly reduced.

The Bacillus velezensis M173 was deposited with the Guangdong Microbial Culture Collection Center with a deposit number of GDMCC No. 61434. After being prepared into a seed-coating agent, Bacillus velezensis M173 can promote the growth and germination of plants and significantly improve the control effect on pathogens. Moreover, Bacillus velezensis M173 can relieve the condition of dead seedlings of crops at seedling stage after being applied to the crops. In particular, both pot experiments and field experiments prove that the Bacillus velezensis M173 of the present invention has a significant control effect on fungal and bacterial diseases, in particular, bacterial wilt and stem rot. Therefore, the Bacillus velezensis M173 of the present invention has broad application prospects.

A method for improving the germination of *Carex lumbricoides* seeds based on nano-zinc oxide initiation belongs to the field of wetland ecological engineering technology. The invention includes the following steps: First, nano-zinc oxide particles with a particle size of 30±10 nm are dispersed in deionized water by ultrasonic oscillation to prepare a suspension of 12.5-200 mg / L; second, collected *Carex lumbricoides* seeds are float-screened, and plump seeds that sink to the bottom are selected and subjected to surface sterilization; subsequently, the sterilized seeds are immersed in the nano-zinc oxide suspension and initiated by agitation under light-proof conditions for 6 or 24 hours, followed by air drying to restore the initial moisture content; finally, the initiated seeds are placed in an artificial climate incubator for germination culture under specific variable temperature and light conditions. This invention utilizes the biocompatibility of nano-zinc oxide and effectively solves the problems of long germination cycles and low germination rates of *Carex lumbricoides* seeds by precisely controlling the initiation concentration and time. This invention has the advantages of simple operation, low cost, and environmental friendliness, and can significantly improve the germination rate and germination percentage, providing high-quality seedlings for the restoration of degraded wetland vegetation.

The application discloses application of eugenol in preparation of a bacteriostatic agent for enhancing symbiotic nitrogen fixation efficiency of legume-rhizobium, and relates to the field of agricultural microorganisms. ‑1 The application is safe and non-inhibiting to seed germination of Medicago polymorpha and target rhizobium LL2 in a concentration range of 500-1000 muL / L ‑1 of eugenol, can significantly reshape rhizosphere microbial community structure by selectively inhibiting competitive microorganisms such as bacillus and streptomyces in soil, and is environment-friendly and free of antibiotic residue risk, thereby providing a green and efficient new approach for improving symbiotic nitrogen fixation efficiency of legume-rhizobium.

The application provides a method for identifying and screening cold tolerance of elephant grass and application thereof, and relates to the field of plant cultivation. The method mainly comprises the following steps: subjecting the standard elephant grass stem segments to variable temperature germination; subjecting the standard elephant grass stem segments to constant temperature low temperature stress; subjecting the stressed stem segments to recovery culture under suitable conditions; determining and calculating the low temperature germination, stress survival rate and recovery growth rate of the stem segments; and comprehensively evaluating the cold tolerance of the stem segments based on the weighted values of the three. The application overcomes the shortcomings of the prior art, can simulate the key low temperature stress and recovery process in the laboratory, and can realize rapid, reliable and batch preliminary identification and screening of the cold tolerance of a large amount of materials, so that the blind dependence on large-scale and long-period field identification is significantly reduced, the screening accuracy and breeding efficiency are effectively improved, and the breeding period is shortened.

This invention relates to a genetic transformation method for Ningxia wolfberry using germinating seeds as explants, belonging to the field of plant genetic transformation technology. The invention mainly includes the following steps: (1) After disinfecting Ningxia wolfberry seeds, inoculate them onto a germination medium, and obtain germinating seeds through vernalization and dark culture treatment; (2) Activate and culture Agrobacterium rhizogenes and Agrobacterium tumefaciens strains carrying target gene expression frames to obtain a bacterial solution for infection; (3) Take the germinating seeds obtained in step (1) and mix them with the bacterial solution obtained in step (2) for infection, then transfer the infected germinating seeds to a co-culture medium or substrate for co-culture; (4) Cultivate the material after co-culture in step (3) to induce root growth, and detect the expression level of the target gene or reporter gene on the roots or plants to obtain transgenic positive plants. This invention has the advantages of simple operation, significantly shortened transformation cycle, and high transformation efficiency.

This invention provides a machine learning-optimized rapid identification model for salt-tolerant maize seeds. By constructing a multi-dimensional dataset encompassing non-contact morphology, minimal physiological stress, and germination digitization features under salt-alkali stress, and combining intelligent preprocessing, mutual information, and bidirectional recursive feature elimination to screen key features, it effectively overcomes the limitations of single indicators. To address the sample imbalance problem, a hybrid sampling strategy is employed; to address feature heterogeneity, a heterogeneous ensemble model fusing CNN and GBDT is designed to process image and structured data separately, with simultaneous parameter tuning through multi-objective Bayesian optimization. This method achieves high-precision, high-generalization prediction of salt-alkali tolerance levels. The identification process is rapid and non-destructive, and the seeds can still be used for breeding. The system supports full-process automation, is suitable for large-scale breeding and commercial testing, significantly improving efficiency and reducing reliance on manual labor.

This invention discloses a method for low-carbon and high-efficiency organic fertilizer production through tiered oxygen supply composting. This invention uses a compound microbial agent as its core, achieving simultaneous breakthroughs in compost maturity and fertilizer efficiency. By precisely delivering targeted compound microbial agents in stages and coupling them with a tiered oxygen supply strategy, this invention directionally regulates the structure and function of the microbial community, significantly improving organic matter conversion efficiency and the degree of humification. The final product shows a significant increase in organic matter content of 31%, and the seed germination index increases from 71% to 89%, demonstrating its excellent effects in thorough composting and eliminating phytotoxicity. In pot experiments, the biomass of maize plants increased by approximately 97%, further verifying the significant promoting effect of high-quality DOM (dominant organic matter) formed by functional microbial agents on fertilizer efficiency.

This invention relates to the field of bioengineering technology and provides an application of the maize heat shock transcription factor gene ZmHSF18, wherein the nucleotide sequence of the maize heat shock transcription factor gene ZmHSF18 is shown in SEQ ID NO:1. Experiments have demonstrated that overexpression of the maize heat shock transcription factor gene ZmHSF18 in plants can significantly improve seed germination rate and frozen survival rate at the mature stage under low-temperature stress, reduce malondialdehyde accumulation, and increase superoxide dismutase activity. Therefore, this maize heat shock transcription factor gene ZmHSF18 can be used to improve plant cold tolerance and create transgenic plants with enhanced cold tolerance, possessing important application value in plant genetic engineering and stress resistance breeding.

The present application relates to oil crop planting technical field, specifically to a kind of rape whole seedling cultivation method, comprising the following steps: carrying out seed screening and sunning treatment, selected planting plot and complete deep ploughing and base fertilizer application;Mix the treated rape seed and millet seed according to fixed proportion, use film mulching hole sowing cylinder to carry out single hole sowing, after sowing, use fine soil to cover and compact seed hole;Monitoring field emergence, seedling grows to two to three leaf stage, spray triclopyr herbicide to remove millet seedling, according to soil moisture and seedling condition, carry out water and fertilizer management;Using the way of combining physical and chemical, prevent and cure disease and insect pest during the growth period of rape.The present application breaks the soil pressure and soil hardening obstacle for rape seed by the strong topsoil capacity of millet seed germination, matches film mulching soil conservation, makes the emergence rate of rape increase from less than 70% to more than 90%, and the topsoil failure rate decreases to less than 5%.

The utility model belongs to the technical field of seed germination observation, especially is involved in an agricultural seed germination observation box. The technology includes the box, be provided with a plurality of baffle in the box, the baffle divides the box into a plurality of for placing the compartment of seedling tray, the top of box is covered with the installation shell, is equipped with a plurality of first through -hole that communicates with the installation shell connection in the box, two pieces of reflector plate are fixed in the top of installation shell, the left and right inner side wall of installation shell all are equipped with the light source board, the front reflector plate is used for the light of left light source board reflection into the box, the back reflector plate is used for the light of right light source board reflection into the box. Start light source board, two pieces of light source board emit light, the light is reflected and irradiated into the compartment through the reflector plate in the installation shell, only two pieces of light source board are used in a box, compared with the light source board of each compartment of existing installation reduces the cost. Through the light reflection and the heat conduction principle of water to improve the automation of seed germination observation box.

This utility model discloses an integrated machine for laying double-layer plastic film and drip irrigation tape, comprising: a frame; a drip irrigation tape placement shaft mounted on the frame, with two drip irrigation tape rolls sleeved on the placement shaft; a white plastic film placement shaft mounted on the frame, with a white plastic film roll sleeved on the white plastic film placement shaft; a black plastic film placement shaft mounted on the frame, with a black plastic film roll sleeved on the black plastic film placement shaft; and a film pressing shaft assembly mounted on the frame. This integrated machine can lay black and white plastic film and drip irrigation tape completely in one operation, which not only improves agricultural efficiency, but also allows the black plastic film to inhibit weed growth, the laid white plastic film to lower the soil surface temperature and prevent seeds from being burned by high temperatures, while also helping to maintain soil moisture and promote seed germination. The laid drip irrigation tape achieves water conservation and eliminates the need for manual watering.

This invention discloses a strain of *Dendrobium nobile* D30D that promotes seed germination and seedling formation, as well as plant growth, and its applications. The strain D30D has the accession number CCTCC NO: M 20252856. Strain D30D can rapidly promote the germination of *Dendrobium nobile* seeds into protocorms and seedlings, promote the growth of *Dendrobium nobile*, and / or increase the medicinal components of *Dendrobium nobile*, demonstrating its significant comprehensive application potential.

This invention belongs to the field of agricultural microbiology technology and relates to a strain of Bacillus velezensis S-34 and its application in controlling mango leaf anthracnose. It was isolated from the rhizosphere soil of the anthracnose-resistant mango variety 'Jinhuang' and deposited at the China Center for Type Culture Collection (CCTCC) on May 29, 2025, with accession number CCTCC No. M 20251225. The Bacillus velezensis S-34 provided by this invention not only controls mango leaf anthracnose but also exhibits good antagonistic effects against various mango leaf pathogenic fungi. Furthermore, Bacillus velezensis S-34, its sterile fermentation broth, and volatile gases can inhibit the germination and epidermal penetration of Anthracnose spores, thereby inhibiting the infection process of Anthracnose spores on plants. This invention provides a new biocontrol agent and preparation method for the efficient control of mango leaf anthracnose and other foliar diseases in agricultural production, which is beneficial to the green and healthy development of the mango industry.

The application discloses application of ZmGOLS2 genes and ZmRAFS genes in improving pollen vitality of corn under heat shock and drought conditions, and belongs to the technical field of genetic engineering. The application improves the pollen vitality of corn, promotes the germination of pollen under heat shock and drought stress conditions, and reduces the empty stalk rate of corn under drought stress conditions by simultaneously improving the expression levels of a corn myo-inositol galactoside synthetase coding gene and a corn raffinose synthetase coding gene.