7305 results about "Germination" patented technology

The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. In one embodiment, a high-throughput, non-destructive method for analyzing individual seeds in a population of seeds comprises removing a sample from a plurality of seeds in the population while preserving the germination viability of the seed and analyzing the sample for the presence or absence of one or more characteristics of at least one genetic or chemical trait.

The invention discloses a breeding method for aseptic seeding and test tube seedling formation of orchids and the adopted broad-spectrum culture medium. In this method, a healthy orchid mother plant is selected, and artificially pollinated when it blooms, and the fruit that develops to maturity after pollination and has not cracked is used as an explant, or the orchid fruit that is basically mature and not cracked is taken from the wild as an explant. Implants undergo reproductive steps such as aseptic sowing, seed germination, strong seedling cultivation, and test-tube seedling transplantation. The specially prepared medium is used for aseptic sowing and seed germination to obtain small plants, and then the small plants are cultivated on the specially prepared strong seedling medium, and the seedlings are hardened under natural light in the greenhouse for 7-14 hours before being released from the bottle. One day, the test-tube seedlings were taken out, the root medium was washed off, and they were planted in a mixed substrate of bark, bluestone and peat, and further cultivated into seedlings. The invention has the characteristics of high seed germination rate, fast seedling growth, good seedling quality, low cost, etc., can obtain a large number of test-tube seedlings in a short period of time, and the transplanting survival rate of the seedlings can be kept above 90%. An effective way can be provided for the production of orchid seedlings.

The invention relates to a method for accurate fertilization for apple tree. The method decides target yield according to soil fertility based on 4000 kg of high quality organic fertilizer, then determine fertilizer consumption according needed fertilizer, fertilizer supply ability by soil and fertilizer availability, dressing all organic fertilizer, 70% of the nitrogen fertilizer and phosphatic fertilizer and 50% of potassium fertilizer as base fertilizer, taking left fertilizer for additional dressing, dressing the left 30% nitrogen before germination, dressing the left 30% phosphatic fertilizer before flower bud differentiation, dressing left potassium fertilizer during fruit expanding period, spraying fertilizer on leaf in different growing period for 6- 8 times in one year. The productivity and output value are increased by 21.5% and 21.3% respectively, the high- quality fruit rate is increased from 35% to 62%,and the net value is added by 615 yuan per mu.

Provided is a bletilla striata rapid propagation seedling cultivation method. Fermented bark and a mixture of humus and river sand serve as a base material, the base material is laid on a seedling cultivation vessel, 1-2 layers of gauze is laid on the base material, water is poured to soak the base material, bletilla striata seeds are sown on the gauze, a transparent plastic film is utilized to wrap the seedling cultivation vessel, and the seedling cultivation vessel is placed in an artificial intelligent cultivation box to grow seedlings and strengthen the seedlings. The bletilla striata rapid propagation seedling cultivation method breaks through a traditional corm planting and cultivation mode of bletilla striata, the quality degeneration problem existing in vegetative propagation is resolved, and a new path is created for sexual propagation seedling cultivation of the bletilla striata genus of orchidaceae. The bletilla striata rapid propagation seedling cultivation method is simple in operation, low in cost and short in seedling cultivation period; the germination rate reaches up to 90%, the planting percent is 70%-80%, and good practical popularization significance is provided for large-scale propagation seedling cultivation of the bletilla striata.

Polynucleotides and polypeptides incorporated into expression vectors have been introduced into plants and were ectopically expressed. The polypeptides of the invention have been shown to confer at least one regulatory activity and confer increased yield, greater height, greater early season growth, greater canopy coverage, greater stem diameter, greater late season vigor, increased secondary rooting, more rapid germination, greater cold tolerance, greater tolerance to water deprivation, reduced stomatal conductance, altered C/N sensing, increased low nitrogen tolerance, increased low phosphorus tolerance, or increased tolerance to hyperosmotic stress as compared to the control plant as compared to a control plant.

A high-throughput method is provided for analyzing individual seeds in a population of seeds. The method generally includes removing a tissue sample from at least one or more seeds in the population of seeds while preserving germination viability of the at least one or more seeds from which the tissue sample is taken, depositing the tissue sample into an individual compartment of a sample tray, contacting the tissue sample with an extraction buffer to remove DNA from the sample, and analyzing the extracted DNA for the presence or absence of a transgene of interest. The extraction buffer may be present in the individual compartment of the sample tray before the tissue sample is deposited into the individual compartment.

The invention discloses a method for growing watermelons. The method includes the steps of processing seeds and accelerating germination, sowing and raising seedlings, applying base fertilizer on the whole land, transplanting the seedlings, performing field management, harvesting and the like. The method for growing watermelons is easy to operate, convenient to manage, good in growth vigor of watermelons, high in disease resistance, uniform in result nature and high in yield, the yield per mu can reach 4000 kg to 5000 kg, the watermelons are large, good in quality, high in sugar content, bright in flesh color, delicate in flesh and high in water content and commodity value, and therefore the method has great significance for increasing income of melon farmers.

The invention discloses a drought-resistant energy crop seed pelleting agent, a preparation method and a using method thereof. The seed pelleting agent comprises the following components: straw powder or overrotten organic matter, a plant growth regulating substance, a water absorbent resin, a filler, an adhesive, an insecticide, a bactericide, urea and monopotassium phosphate. The method for seed pelleting comprises the following steps of: 1) spraying an adhesive water solution on the surfaces of drought-resistant energy seeds evenly; 2) mixing the drought-resistant energy seeds of which the surfaces are the adhesive water solution and a pelleting agent for coating; and 3) repeating the step 1) and the step 2) until the diameters of the seeds meet the requirement. The invention breaks the traditional idea that the pelleting agent absorbs water in soil of a plough layer, establishes a theory of absorbing water before the sowing and sowing after the germination acceleration, and realizes the aim that the pelleting agent can absorb the water before the sowing after the coating, does not disperse after the water absorption, has good expansibility, ensures that the seeds germinate in the palleting agent and can adopt mechanized sowing by adding crop straw powder or the overrotten organic matter which is rich in water absorption expansibility into the components of the pelleting agent.

The invention discloses a mechanically-mulching cultivation method of organic rice, relating to a rice cultivation method. The purpose of the method provided by the invention is to solve the weeding problem in paddy fields in the existing cultivation method of organic rice. The method comprises the steps of 1, selecting field environment, namely (1) selecting soil, (2) selecting air quality and (3) selecting a water source; 2, performing soil preparation in the field, namely (1) ploughing, (2) fertilizing and (3) digging canals and harrowing; 3, preparing seedbed and bed soil, namely (1) preparing seeding environment, (2) preparing seedbed and (3) preparing bed soil; 4, germination treatment of seeds, namely (1) drying seeds, (2) performing plasma treatment, (3) soaking seeds and (4) accelerating germination; 5, sowing; 6, managing seedlings, namely (1) performing insect prevention treatment, (2) managing temperature, (3) managing water, and (4) fertilizing; 7, mulching, transplantingseedlings and managing the field, namely (1) making a bed and mulching, (2) transplanting, (3) irrigating, (4) dressing foliar fertilizer, (5) managing water layers in the field, (6) weeding and (7) preventing insects. The method is mainly used for cultivating organic rice by mechanical mulching.

A floatable growth tray for the germination of seedlings has a plurality of cells adapted to receive a mixture of a growth median and seeds. The tray has sealed pockets forming air chambers to enable the tray to float. The cells have holes forming openings extending through a bottom surface of the tray permitting water to enter the cells to promote germination of the seeds.

A method and apparatus for increased yields of water-soluble polyphenols from plant seeds is provided. The method includes inducing seed germination within a selected range of temperatures. The method also includes inducing germination in an atmosphere having elevated carbon oxygen concentration and/or a reduced concentration of oxygen. The method further includes exposing the seeds to light and extracting water-soluble polyphenols with an aqueous solution without adding an organic solvent such as alcohol.

The invention discloses a method for the in-situ dynamic observation and the measurement of the root system of a plant, which comprises the following steps: designing a transparent cultivation container and a growth medium; sterilizing seeds, accelerating germination, moving seedlings to the transparent cultivation container after budding, and keeping the natural growth of the root system of the plant in the transparent growth medium; scanning the root system by a three-dimensional laser scanning instrument, carrying out the in-situ observation and the image acquisition of the three-dimensional configuration of the root system and completing the steps of image collection, reconstruction, simulation and the like. By the three-dimensional visualization analysis of the in-situ growth condition of the root system of the plant, the invention directly reflects the three-dimensional growth condition of the root system, does not need destructive detection and realizes dynamic observation.

There is provided a packaging container which reserves an acidic beverage having a relatively high acidity and allows spore to remain properly without corrupting and the acidic beverage can be aseptically filled and reserved at a normal temperature with low cost without utilizing a container having high heat-proof property and expensive manufacturing equipment. An interior of a container 2 is sterilized by a sterilizing agent “b” and a heated water “c” so as to allow bacterial spore to be alive but not to allow bacteria vegetative cell, mold and yeast to be alive, and a sterilized content “a” having an acidity of an extent of suppressing germination of the bacteria spore fills the container at a normal temperature or low temperature, and then the container is sealed by a lid 3, thereby providing a packaging container 1.

The invention discloses an early-spring transplantation and seedling protection method for facility-grafted jujube seedlings and belongs to the technical field of plant seedling cultivation. The method comprises the following steps: disinfection and sterilization, root system awaking, root and head protecting, planting pit fertilizing, seedling planting and temperature control management. Compared with the prior art, the survival rate of the seedlings treated with the method is increased by 15.38 percent when compared with that of seedlings treated with the conventional planting seedling protection technology, and jujube trees can germinate 5-10 days in advance. The jujube seedlings are transplanted before the germination stage, and various technical measures are combined for seedling protection. The germination rate of the jujube seedlings is advanced and promoted, and the survival rate of the jujube seedlings is ensured and increased. The established practice that the survival rate is too low due to untimely jujube tree transplantation in spring is broken down, and the method lays a foundation for thriving of facility early-spring jujube seedlings. The method can be widely applied to planting seedling protection of seedlings of fruit trees and woods.

The invention relates to a technique of ginseng planting, in particular to a method for planting ginseng on a farmland, which includes: land selection, soil improvement, bed making, seed sprouting, seeding, transplanting, shed erection, field management, overwintering cold protection, and ginseng root harvesting. By improving and regulating relevant technical steps and parameters in ginseng planting, the method is more reasonable and highly operable, regulated planting is achieved, farmland soil quality is improved, the requirements of ginseng cultivation are met, ginseng quality is guaranteed, and popularization of ginseng planting on farmlands is benefited. The sprouting is achieved by the sand and water process, seed opening rate reaches more than 90%, and germination rate is increased. Annual ginseng seedlings are used for transplanting, the transplanted seedlings continue growing for 4-5 years on the same farmland, and accordingly land resources are saved, ginseng growth and double-tuber ginseng formation are benefited, photosynthetic area is enlarged, and ginseng quality and yield are increased.

This invention provides methods, devices, and kits for growing a plant or germinating a seed into a plant; methods and devices for delivering oxygen to a plant or a seed which will germinate into a plant; methods and devices for delivering liquid to a plant; methods and devices for increasing the dissolved oxygen concentration in a liquid to be delivered to a plant; methods and devices for increasing the dissolved oxygen concentration in a liquid within a hydroponics device; terraced oxygenators; aspirators, downdraft venturis, net baskets; germination caps, sets of germination caps; methods for increasing the likelihood of germination of a seed; seed-bearing support media; methods for germinating a seed; and smart garden devices for hydroponics growing systems.

The invention relates to a light control method for dendrobium officinale tissue culture, which comprises the following steps of: obtaining powdery seeds after dendrobium officinale fruits are sterilized, culturing the powdery seeds through a seed germination culture medium, and germinating the cultured seeds to form protocorms. The method is characterized in that: in the tissue culture process of dendrobium officinale seedlings, a light emitting diode (LED) plant growth illumination system is used as a light source, and different illumination treatment is implemented according to different stages of dendrobium officinale tissue culture. The different illumination treatment is implemented according to different growth stages so that the dendrobium officinale seedlings can furthest utilize light energy for photosynthesis and the highest growth amount is obtained. Experimental results show that: the culture period of the dendrobium officinale seedlings can be effectively shortened according to the technical scheme, the time from the seeds to the rooted finished seedlings is only 150 to 180 days, the growth period is shortened by 30 to 60 days compared with the culture under a common fluorescent lamp, and the seedlings cultured by the method have stable hereditary character and good quality.

The invention provides a cuttage and propagation method of a twig of cyclocarya paliurus, which comprises the following steps: (1) cutting trunks and promoting the germination of a scion seed tree from the middle ten days of March to the middle ten days of April; (2) cutting a semi-lignified twig as a cutting spike from the first ten days of June to the first ten days of August, wherein the length of the twig is 10cm to 15cm, 5 to 6 small leaves are remained at the upper part of the twig, and the lower end of the twig is cut into oblique section; (3) dipping 500mg/L to 800mg/L of paste-like root growing agent of indolebutyric acid at the positions of 3cm to 4cm of the lower end of the cutting spike; (4) inserting the cutting spike in a light substrate consisting of carbonized pine bark and sawdust, wherein the cuttage depth is 4cm to 5cm; and (5) shading the cutting spike by a black sunshade net with the shading degree of 50% to 70% after the cuttage, and spraying and moisturizing the cutting spike by adopting an intermittent spraying method. 60 days after the cuttage, the rooting rate reaches over 80%. The method is simple and practical to operate and is suitable for large-scale cuttage; seedlings can effectively keep the excellent inherited characteristics of the seed tree; moreover, the effect of the quick vegetative propagation of improved seeds can be achieved.

The invention relates to a method for planting Suaeda salsa on serious saline-alkali soil by trickle irrigation in arid areas, and the method comprises the following steps: overlaying an irrigation facility in a heavy salinization soil environment; arranging hollow billet trickle irrigation zones in a mode of one pipe in one row; in a strip cropping mode, planting halophyte seeds on two sides of the hollow billet zone; dripping after sowing is finished to prevent seeds from being blown by wind; and combining with a water-saving trickle irrigation technology, causing soluble salt in the areas near the trickle irrigation billet trickle zone to transfer along with water or transfer to direction far away from the billet trickle by controlling the trickle irrigation flow so as to create a low-salt environment for halophyte seeds to germinate and young seedlings to grow, thereby planting the young seedlings. According to the method disclosed by the invention, inhibition on halophyte seed germination and young seedling growth by the heavy salinization soil environment can be overcome, a technical mode for developing the agricultural production on the common big rinsing salt improved saline-alkali soils can be broken through, and ecological economy halophyte is planted on a large scale so as to accumulate technical reserve for developing and utilizing the large-area saline-alkali soilwasteland in arid areas.

The invention relates to a method for cross breeding and seedling propagation of paphiopedilum, which comprises the steps of artificial pollination, sterile seeding, strong seedling culture, and test-tube plantlet transplant. After the artificial pollination of paphiopedilum coenospecies, a powdery embryo in hybrid fruits is inoculated to a specially prepared seed germination culture medium for culture; plantlets obtained by the sterile seeding are inoculated to a specially prepared strong seedling culture medium for strong seedling culture; and finally, the test-tube plantlet transplant is performed. The method uses unique culture media, ensures the high germination rate, quick seedling and high seedling quality of paphiopedilum hybrid seeds, has the advantages of high operability, high application value and the like, and overcomes the difficulty that the paphiopedilum hybrid seeds are extremely difficult to germinate in a natural state due to the incomplete development of embryo and other difficulties.

The invention discloses a wind prevention and sand fixation greening planting technology with good sand control effect. The wind prevention and sand fixation greening planting technology mainly comprises the following steps of: firstly arranging a plurality of strip sand-protecting barriers in a traveling dune zone; then mix-seeding herbaceous plant seeds in open grounds among the strip sand-protecting barriers, and planting shrub seedlings at the positions where the strip sand-protecting barriers are located; and finally fertilizing among the strip sand-protecting barriers and inside the strip sand-protecting barriers. According to the wind prevention and sand fixation greening planting technology, normal germination growth of most herbaceous plant seeds and shrub seedlings can be ensured without planting by multiple times; moreover, the growth period of herbaceous plants is relatively short, so that grass capable of sand fixation can be formed in a short time to further ensure normal growth of shrub seedlings; a grass and shrub forest can be formed in a relatively short time, two to three years in general, through the combination of the sand fixation of sand-protecting barriers with grass and shrub plants so as to achieve the effects of wind prevention and sand fixation; and the desertification of land can be effectively prevented. The technology is suitable for popularization and application in the technical field of sand control.

The invention discloses a method for directly seeding rice, which comprises the following steps of: preparing soil; preparing a slag fertilizer; soaking seeds and accelerating germination; digging holes and seeding in a dry field; weeding; fertilizing in a land for growing field crops; thinning; and preventing and controlling plant diseases and insect pests. Since the method for directly seeding the rice directly digs holes and seeds in the field, the method can ensure that rice in a dry area grows normally by using a little amount of water before the head sprouting process of the rice, achieves good drought-resisting and water-saving effects, eliminates the transplanting step, avoids damaging root systems of the rice, strengthens the tillering capacity of the rice, achieves high yield, simplifies the working procedure, saves labor force, has low production cost, high efficiency and convenient operation, and can be widely used for planting rice in mountain areas, hilly areas, easily droughty areas and water-lacking dead angles of pieces of paddy fields.

The invention discloses a production method for a paraffin section of a paeonia lactiflora mature embryo. The production method comprises the following steps: drawing materials and fixing: stripping a seed coat, cutting a seed, immersing the seed top part containing the embryo or part endosperm into a FAA stationary liquid, and fixing for more than 24 hours; dehydrating: washing in ethyl alcohol with the concentration of 70% for 3 to 5 times, and performing gradient dehydration from the ethyl alcohol with the concentration of 70%; performing hyalinization: carrying out five level hyalinization by using dimethyl benzene as a transparent reagent; waxing and embedding: pouring out one half volume of dimethyl benzene, adding wax crumbs in a bottle until saturation, staying overnight at the temperature of 40 DEG C; then changing a wax liquid every two hours for three times per day, repeating for three days, and carrying out conventional paraffin embedding; slicing; flattening and bonding: flattening a linoleum tape on an iron plate of a water bath kettle at the constant temperature of 50 DEG C, dewaxing, rehydrating, dyeing through safranine and fast green and performing hyalinization; mounting. According to the invention, the paeonia lactiflora mature embryo paraffin section of which the tissue structure is integrated and clear can be obtained, so that accurate technical support can be provided for researching paeonia lactiflora seed dormancy and germination.

The invention discloses a method for improving paris polyphylla var. yunnanensis seed germination rate. The invention relates to the technical field of seed biology. The method is characterized in that: collected ripe paris polyphylla var. yunnanensis fruits are dried in shade with the peels; testa is removed by kneading; seeds with the testa removed are subjected to high-temperature soaking, wherein the seeds are soaked for 48h in water with a temperature of 40 DEG C; seed coat injury treatment is carried out after high-temperature soaking, wherein the soaked seeds are placed on a rough metal plate, and the seed coats are rubbed; the seeds are soaked by using gibberellin, and variable-temperature sand preserving treatment is carried out. According to the invention, a unique method for promoting seed germination is effectively integrated with a traditional paris polyphylla var. yunnanensis seed germination promotion method, such that a paris polyphylla var. yunnanensis seed germination rate can be higher than 96% which is increased by 35.2% than that of an existing comprehensive seed germination method.

The invention discloses a novel strain CLIMAX-VSF of streptomyces fradiae. The preservation number of the novel strain is CGMCC No. 5133; the preservation date is August 12, 2011; and the preservation department is China General Microbiological Culture Collection Center. The invention further discloses a microbial inoculum containing the novel strain. The novel strain CLIMAX-VSF of the streptomyces fradiae, which is provided by the invention, has quite obvious performances of disease resistance, nitrogen fixation, phosphate solubilization and somatotropin secretion. By using the strain, the germination rate and the growth speed in a seedling stage can be obviously improved under the condition that nutrients are not added to soil, and furthermore, diseases in the seedling stage and a growth period are obviously inhibited. On the aspect that the strain is used for the mixed preparation and the synergy of a fertilizer, the strain shows to have the favorable effect of activating soil nutrients and the performance of resisting a plurality of cultivation obstacles.

The present invention discloses a tobacco cultivation method. Said method is characterized by that it adopts a molecular biological technique to identify key gene capable of promoting axillary bud germination and growth after the tip is pruned, then uses nocuity inducible promoter to drive antisense RNA and make it promptly express after the tip is pruned so as to inhibit the expression of said key gene capable of promoting axillary bud germination and growth and make the axillary bud can not be germinated or grown.

The invention discloses a method for culturing pleurotus eryngii. The method comprises the following steps of: selecting saw powder of alfalfa growing in organic soil, wild nuts in mountain and wild trees in mountain, mixing, fermenting, sterilizing, and inoculating the sterilized fermentation product to pleurotus eryngii hypha to culture to obtain mature pleurotus eryngii. By adopting the alfalfa growing in the organic soil, the problem that the alfalfa growing in common soil contains chemical fertilizer, pesticide residue and the like is solved, and harm of chemicals to a human body is solved; meanwhile, because the alfalfa is selected as one of the culture medium materials for culturing the pleurotus eryngii, the germination rate of the pleurotus eryngii can be increased by using the alfalfa to culture the pleurotus eryngii; and because the alfalfa is used as one of the culture medium materials, the growth speed of hypha can be increased, so that the production efficiency of the pleurotus eryngii is increased, and the economic benefit of the pleurotus eryngii is increased. The method has the advantages of achieving the best sterilization effect, saving the sterilization time, being simple and easy in material selection, saving a large amount of labor, reducing the production cost and increasing the economic benefit.

The invention relates to a planting method of Chinese medicine, namely a standard planting method of non-forest ginseng. The method comprises the following steps: (1) land selection; (2) soil improvement; (3) selection of ginseng seeds and germination treatment; (4) making a soil bed; (5) seeding; (6) erecting a frame; (7) collecting seedlings; and (8) transplanting the seedlings and the like. The planted ginseng is transferred from a wild mountainous region to a non-forest flat land; and all links from land selection, soil detection and soil improvement to the selective application of a fertilizer of the improved soil, the application of the key technology of each production link, the ginseng preparation and the like comfort to the Chinese medicine GAP (good agricultural practice) production and quality management standard and standard production operation procedure, and the production and quality safety of the non-forest ginseng is guaranteed; and the technology not only can effectively protect the ecological environment and powerfully improve the land utilization rate, but also can solve the problems of pesticide residue and super-standard heavy metal content in the traditional ginseng planting process, thereby ensuring the quality of the ginseng raw medicine product.

A plant growth regulator containing glutathione allows increasing harvest index. This provides a technique for specifying a control factor for a plant, thereby effectively controlling germination, growth, anthesis etc. of the plant.

The invention belongs to the field of microbial resources, and particularly discloses a strain of Bacillus aryabhattai J5 and application thereof in promoting growth of corn. The Bacillus aryabhattai J5 is collected in CGMCC (China General Microbiological Culture Collection Center) on October 15th, 2015 and is filed with the number of CGMCC NO.11508. The Bacillus aryabhattai J5 has functions of producing auxin, siderophore, ammonia and protease, dissolving phosphorus, resisting to salt, realizing antagonism, and the like. As the strain is capable of growing normally in a culture medium with salt content equal to 11%, the strain has good potential in eliminating soil salinization. As content of siderophore produced by the strain is up to 53.7%, the strain has effective disease-prevention property. By the strain, corn seed germination rate can be increased by 12.5%, and accordingly the strain has good growth promoting effect. By application of microbial inoculum prepared from the strain, corn yield is increased by 37.1%, and hundred-grain weight is increased by 35%. It is possible to develop the strain into microbial inoculum, and accordingly the strain has wide application prospect in terms of improving soil nutrient, eliminating salinization, promoting growth of plants, preventing diseases and the like.