10 results about "Orchidaceae" patented technology

The present invention relates to the field of flower branch propagation, and in particular to a cutting propagation method for Orchidaceae and a special cutting potted plant. The cutting propagation method for Orchidaceae of the present invention mainly promotes the germination of the stems and roots of Orchidaceae by using hormones and EM compound probiotics, thereby achieving a seedling propagation method, saving the tedious tissue culture seedling process, saving the risks of raw materials and tissue culture explant disinfection, and being easy to operate, low in cost, and short in cycle. Compared with orchid tissue culture seedlings, the cutting potted plant has the advantages of a higher growth rate and a faster rooting rate. In addition, the cutting potted plant specially used for Orchidaceae cutting propagation of the present invention can automatically adjust the combined covering state of the main shade net and the auxiliary shade net according to demand, providing an intelligent cultivation environment for Orchidaceae cutting propagation based on the application of the cutting propagation method for Orchidaceae of the present invention.

The invention discloses a coprinus comatus strain for promoting seed germination of cremastra appendiculata and application of the coprinus comatus strain, the strain is separated and screened from root tissues of cremastra appendiculata, the strain is identified as coprinus comatus through ITS (internal transcribed spacer) sequencing, the Latin name is Coprinella micaceus, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO: 42051. The strain is inoculated into a liquid culture medium for shake culture, and mycelia are collected and mixed with sterile vermiculite and turfy soil to prepare a fungicide; the method comprises the following steps: laying a sterilized culture medium formed by mixing leaf mold, perlite and bark in a sterile culture container, spreading a fungicide, spreading the treated azalea seeds, covering the culture medium, and culturing by controlling the temperature, the illumination condition and the relative humidity. According to the method, the germination rate of the rhododendron simsii seeds can reach 60%-70%, the germination time is shortened to 15-20 days, the survival rate of seedlings is high, operation is easy and convenient, cost is low, the method is suitable for large-scale breeding and resource protection of the rhododendron simsii, and a new thought is provided for research on symbiotic germination of orchid plants.

The invention relates to the technical field of Tibetan medicines, in particular to a preparation containing Tibetan medicine musk, a preparation method of the preparation and application of the preparation in preparation of liver cancer medicines. The preparation is prepared from the following raw materials: musk, swertia mussotii, rheum tanguticum, dracocephalum tanguticum, delphinium delphinium, phyllanthus emblica, salviae miltiorrhizae, herpetospermum pedunculosum, saussurea hypoglauca, saffron crocus, trogopterus dung, fructus chebulae and plantago asiatica. According to the invention, a plurality of medicinal materials are compatible, all the raw materials have a synergistic effect, the effects of compound components are effectively exerted, and the traditional Chinese medicine composition can resist cancer, protect liver, clear away heat and toxic materials and inhibit tumor growth, has a good anti-liver cancer effect and does not generate an immunosuppressive effect on a body.

The invention provides a method for rapidly transferring pleione bulbocodioides after sterile sowing, and relates to the technical field of tissue culture, the method comprises the following steps: providing pleione bulbocodioides pods; providing a primary culture medium; carrying out pod sowing and primary culture; subculture transfer: pouring the primary culture medium full of the protocorms into a device containing a subculture medium, and spreading the primary culture medium. According to the method, the transfer of the subculture is completed by pouring and sub-packaging, so that the waste of a primary culture medium is avoided, the time required by the transfer of the subculture tissue material is shortened, the time cost of seedling production is effectively reduced, and meanwhile, the yellowing or differentiation blocking of protocorm caused by deterioration of a microenvironment in a tissue culture bottle due to an overlong transfer period is avoided; the subsequent seedling production quality is improved; compared with a traditional mode of transferring by using tweezers, the method has the advantages that the time of exposing the tissue material in an environment outside a bottle is greatly shortened, and the pollution rate of the tissue material is effectively reduced.

The invention belongs to the technical field of medicine, and discloses a glucomannan structure, a preparation method thereof and application of the glucomannan in antitumor drugs, the glucomannan is composed of mannose (Man) and glucose (Glc), the molar ratio of the mannose to the glucose is an integer between 1.0 and 4.0: 1.0, the mannose and the glucose are mainly connected through a 1-4 beta bond (the following structural general formula is shown in the specification), and the molecular weight range is 3.0 * 10 < 4 >-9.1 * 10 < 5 > Da. The glucomannan is uniform polysaccharide obtained by performing ethanol degreasing, water extraction and alcohol precipitation and Sevag method deproteinization on dried pseudobulbs of orchid pleione bulbocodioides and Yunnan pleione bulbocodioides and then adopting anion exchange and gel chromatography methods, and the structure of the glucomannan is represented by UV, IR, HPLC, GC-MS and NMR technologies. Animal in-vivo tests show that the glucomannan can effectively inhibit the growth of mouse breast cancer, has small toxic and side effects, has the effect of treating solid tumors, and can be used as an anti-tumor polysaccharide drug. # imgabs0 # comprises: m is 1 or 2, and n is an integer between 22 and 617; r1 and R2 are-OH or CH3COO <->.

To provide a skin care preparation which is derived from a natural product, is excellent in biological safety, and exhibits functions of preventing or improving skin roughness, improving the transparency of the skin, and suppressing cell aging by suppressing the secretion of inflammatory substances (cytokine and chemokine) in the skin.SOLUTION: An extract of Gentiana lutea belonging to the genus Gentiana of the family Gentianaceae and an extract of Bletilla striata belonging to the genus Bletilla of the family Orchidaceae are used as active ingredients for suppressing the secretion of inflammatory factor (cytokine and chemokine).SELECTED DRAWING: None

The present application relates to the technical field of medicinal plant tissue culture, in particular to a rapid virus-free cloning method and reagent combination for medicinal plant tissue culture. The method comprises the following steps: pretreatment and disinfection of explants, precise virus elimination, preparation of dynamic adaptive medium, rapid propagation, real-time high-throughput detection of virus-free, seedling raising and transplanting, etc. The reagent combination comprises a multifunctional degradable reagent kit, intelligent response type nano disinfection microcapsules, special reagents such as composite virus elimination liquid, etc. The virus elimination rate is greater than or equal to 95%, the survival rate of explants is greater than or equal to 90%, the propagation cycle is shortened by 30%, the detection time is less than or equal to 2 hours, the survival rate of transplanting is greater than or equal to 85%, and the degradation rate of reagents is greater than or equal to 90%. The present application is suitable for herbaceous, woody and liana medicinal plants of the families of Araliaceae, Eucommiaceae, Orchidaceae and Campanulaceae, etc., can realize stable virus elimination and propagation effect, significantly improve the virus-free cloning efficiency and seedling quality, and meet the green production demand.

The invention discloses a flavonol synthase gene CxFLS of impatiens biennis and an application of the flavonol synthase gene CxFLS. A gene with the full length of a gene coding sequence of 1002bp is cloned from'impatiens yunnanensis' for the first time, the gene is a flavonol synthase CxFLS gene, heterologous expression is performed by constructing an overexpression vector, CxFLS is shown to negatively regulate and control the anthocyanin content, and the higher the expression quantity of CxFLS is, the lower the anthocyanin content is and the shallower the flower color is; overexpression of CxFLS in tobacco finds that the flower color of transgenic tobacco becomes light or even white, pure white flower tobacco can be produced, the transgenic tobacco can be used for flower color improvement of ornamental plants, the overexpression CxFLS can be used for constructing light-colored flower plants and rapidly obtaining pure-colored flower materials, the problems that flower color improvement methods such as cross breeding and mutation breeding are long in period and not accurate are solved, and the method is suitable for popularization and application. By regulating CxFLS expression to cultivate varieties with light flower colors, the method has important significance on flower color breeding of ornamental plants.

The present invention discloses a coding sequence for the AP1 flowering regulatory gene in Chinese orchids and its application. This orchid floral organ development regulatory gene was isolated from the floral organ cDNA of the Chinese orchid cultivar 'Bai Mo'. Increasing its expression can cause loss of apical dominance in Arabidopsis thaliana, an increase in lateral inflorescences, irregular arrangement of florets on the inflorescence axis, and abnormal petal development in flowers, with the occurrence of fewer petals or petals transforming into sepals. In Chinese orchids, a significant positive correlation was found between gene expression and an increase in sepals, and expression was significantly elevated in variants with a tree-forked flower pattern, indicating that this gene promotes sepal development in Chinese orchids. The Chinese orchid flowering regulatory gene disclosed herein can be used to study the molecular mechanisms of floral organ development in orchidaceae plants and improve flowering traits in plants.

The application discloses application of gastrodin in preparation of SCA3 disease treatment drugs, and the gastrodin is a compound extracted from Gastrodia elata Blume of the Orchidaceae family and has therapeutic effects on neurasthenia, headache and the like. Researches show that the gastrodin can significantly reduce inclusion bodies caused by mutant ataxin-3, improve soluble ataxin-3 protein levels, has no toxic side effects on HEK293T cells, and regulates the specific molecular mechanism of inclusion bodies formed by ataxin-3 through an AKT / ERK signal axis. The application aims to reveal the application of the gastrodin in playing a neuroprotective effect on SCA3 diseases, enrich new uses of old drugs, and provide a new idea for prevention and treatment of other neurodegenerative diseases.