18 results about "Orchidaceae" patented technology

The invention relates to the technical field of plant genetic engineering, in particular to a promoter and application thereof in orchid plants. The invention provides an orchid endogenous promoter which is a powerful constitutive promoter and can drive a target gene to be powerfully expressed in various tissues such as roots, stems, leaves and flowers of an orchid. Compared with a CaMV 35S promoter, the promoter has the advantages that the driving efficiency of the gene in orchid plants is greatly improved, an important tool is provided for molecular breeding of plants, and the promoter has a relatively good application prospect.

The present invention belongs to the field of shampoo and hair care products, and provides an anti-hair loss, oil-control and antibacterial composition, a shampoo and a conditioner. The anti-hair loss, oil-control and antibacterial composition provided by the present invention is composed of type XVII collagen, hydrolyzed keratin, Platycladus orientalis leaf extract, Sophora flavescens extract, Forsythia suspensa extract and Artemisia argyi extract in a mass ratio of 0.5:0.5:3:2:1:1. Based on the composition, the present invention also provides an anti-hair loss, oil-control and antibacterial shampoo and a conditioner. The components of the anti-hair loss, oil-control and antibacterial composition of the present invention have good effects in inhibiting hair loss, inhibiting sebum secretion, inhibiting bacteria, etc., and there is a synergistic effect between the components in inhibiting sebum secretion and inhibiting bacteria, which can improve the comprehensive performance of existing shampoo and hair care products.

The present invention relates to the field of flower branch propagation, and in particular to a cutting propagation method for Orchidaceae and a special cutting potted plant. The cutting propagation method for Orchidaceae of the present invention mainly promotes the germination of the stems and roots of Orchidaceae by using hormones and EM compound probiotics, thereby achieving a seedling propagation method, saving the tedious tissue culture seedling process, saving the risks of raw materials and tissue culture explant disinfection, and being easy to operate, low in cost, and short in cycle. Compared with orchid tissue culture seedlings, the cutting potted plant has the advantages of a higher growth rate and a faster rooting rate. In addition, the cutting potted plant specially used for Orchidaceae cutting propagation of the present invention can automatically adjust the combined covering state of the main shade net and the auxiliary shade net according to demand, providing an intelligent cultivation environment for Orchidaceae cutting propagation based on the application of the cutting propagation method for Orchidaceae of the present invention.

The invention belongs to the technical field of traditional Chinese medicines, and particularly discloses a traditional Chinese medicine composition for treating chemotherapy-related hand-foot syndrome, which is prepared from the following raw materials in parts by weight: 50-60 parts of purslane, 20-30 parts of rhizoma atractylodis, 50-60 parts of cacumen biotae, 20-30 parts of radix sophorae flavescentis, 20-30 parts of gallnut, 15-20 parts of dandelion, 30-40 parts of folium isatidis, 20-30 parts of garden balsam stem, 10-15 parts of pericarpium zanthoxyli and 10-20 parts of fructus evodiae. The traditional Chinese medicine composition provided by the invention can obviously improve symptoms such as erythema and damage of hand and foot skins, exuded yellow pus, local skin sclerosis, pain and the like of patients with chemotherapy-related hand and foot syndromes.

The invention discloses a coprinus comatus strain for promoting seed germination of cremastra appendiculata and application of the coprinus comatus strain, the strain is separated and screened from root tissues of cremastra appendiculata, the strain is identified as coprinus comatus through ITS (internal transcribed spacer) sequencing, the Latin name is Coprinella micaceus, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO: 42051. The strain is inoculated into a liquid culture medium for shake culture, and mycelia are collected and mixed with sterile vermiculite and turfy soil to prepare a fungicide; the method comprises the following steps: laying a sterilized culture medium formed by mixing leaf mold, perlite and bark in a sterile culture container, spreading a fungicide, spreading the treated azalea seeds, covering the culture medium, and culturing by controlling the temperature, the illumination condition and the relative humidity. According to the method, the germination rate of the rhododendron simsii seeds can reach 60%-70%, the germination time is shortened to 15-20 days, the survival rate of seedlings is high, operation is easy and convenient, cost is low, the method is suitable for large-scale breeding and resource protection of the rhododendron simsii, and a new thought is provided for research on symbiotic germination of orchid plants.

The invention relates to a preservation method of orchid seeds. The preservation method comprises the following steps: soaking the orchid seeds into a loading solution; replacing the loading solution with a plant vitrification solution, and carrying out a vitrification reaction for 0.25-1.5 h; treating the vitrified seeds at an ultralow temperature; and storing the seeds subjected to the ultra-low temperature treatment. The orchid seeds preserved by the method have high survival rate and germination rate, the method is simple to operate, germplasm resource protection of orchid can be effectively promoted, and diversity of orchid is reserved.

The invention discloses a structure of glucomannan and application of the glucomannan in antitumor drugs, the glucomannan is mainly composed of mannose (Man) and glucose (Glc), the molar ratio of the mannose to the glucose is 1.0-4.0: 1.0, the mannose and the glucose are mainly connected through a 1-4 beta bond (as shown in the specification), and the molecular weight range of the glucomannan is 3.0 * 10 < 4 >-2.0 * 10 < 5 > Da. The glucomannan is a homogeneous polysaccharide obtained by carrying out ethanol degreasing, water extraction and alcohol precipitation and Sevag method deproteinization on dried pseudobulb of orchid rhododendron, and then carrying out ion exchange resin column chromatography, and the structure of the glucomannan is determined through UV, IR, HPLC, GC-MS, NMR and other technologies. Animal in-vivo tests show that the glucomannan can effectively inhibit growth of tumors such as breast cancer and liver cancer of mice, has small toxic and side effects, has an effect of treating solid tumors, and can be used as an anti-tumor polysaccharide drug. Wherein the range of m is from 1.0 to 4.0, and the range of n is from 30 to 920; r1 and R2 are hydroxyl (-OH) or acetyl (CH3COO-).

The invention relates to the technical field of Tibetan medicines, in particular to a preparation containing Tibetan medicine musk, a preparation method of the preparation and application of the preparation in preparation of liver cancer medicines. The preparation is prepared from the following raw materials: musk, swertia mussotii, rheum tanguticum, dracocephalum tanguticum, delphinium delphinium, phyllanthus emblica, salviae miltiorrhizae, herpetospermum pedunculosum, saussurea hypoglauca, saffron crocus, trogopterus dung, fructus chebulae and plantago asiatica. According to the invention, a plurality of medicinal materials are compatible, all the raw materials have a synergistic effect, the effects of compound components are effectively exerted, and the traditional Chinese medicine composition can resist cancer, protect liver, clear away heat and toxic materials and inhibit tumor growth, has a good anti-liver cancer effect and does not generate an immunosuppressive effect on a body.

The invention provides a method for rapidly transferring pleione bulbocodioides after sterile sowing, and relates to the technical field of tissue culture, the method comprises the following steps: providing pleione bulbocodioides pods; providing a primary culture medium; carrying out pod sowing and primary culture; subculture transfer: pouring the primary culture medium full of the protocorms into a device containing a subculture medium, and spreading the primary culture medium. According to the method, the transfer of the subculture is completed by pouring and sub-packaging, so that the waste of a primary culture medium is avoided, the time required by the transfer of the subculture tissue material is shortened, the time cost of seedling production is effectively reduced, and meanwhile, the yellowing or differentiation blocking of protocorm caused by deterioration of a microenvironment in a tissue culture bottle due to an overlong transfer period is avoided; the subsequent seedling production quality is improved; compared with a traditional mode of transferring by using tweezers, the method has the advantages that the time of exposing the tissue material in an environment outside a bottle is greatly shortened, and the pollution rate of the tissue material is effectively reduced.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a composition for promoting hair growth and a preparation method thereof. The composition for promoting hair growth comprises a traditional Chinese medicine extract and wormwood essential oil, and the traditional Chinese medicine extract is prepared from, by weight, 10-20 parts of wormwood, 5-15 parts of ginger, 2-10 parts of angelica sinensis, 2-10 parts of cacumen biotae, 2-10 parts of ligusticum wallichii, 2-10 parts of radix paeoniae rubra, 2-10 parts of saffron crocus, 2-10 parts of peach kernels, 1-10 parts of fructus cnidii, 1-10 parts of borneol and 1-10 parts of begonia yunnanensis. 1 to 10 parts of pearl powder and 1 to 10 parts of lagotis brevituba. The composition disclosed by the invention can obviously promote hair growth and maintain the physiological structure stability of hair follicles, and has important significance on hair growth and hair follicle protection.

The invention belongs to the technical field of medicine, and discloses a glucomannan structure, a preparation method thereof and application of the glucomannan in antitumor drugs, the glucomannan is composed of mannose (Man) and glucose (Glc), the molar ratio of the mannose to the glucose is an integer between 1.0 and 4.0: 1.0, the mannose and the glucose are mainly connected through a 1-4 beta bond (the following structural general formula is shown in the specification), and the molecular weight range is 3.0 * 10 < 4 >-9.1 * 10 < 5 > Da. The glucomannan is uniform polysaccharide obtained by performing ethanol degreasing, water extraction and alcohol precipitation and Sevag method deproteinization on dried pseudobulbs of orchid pleione bulbocodioides and Yunnan pleione bulbocodioides and then adopting anion exchange and gel chromatography methods, and the structure of the glucomannan is represented by UV, IR, HPLC, GC-MS and NMR technologies. Animal in-vivo tests show that the glucomannan can effectively inhibit the growth of mouse breast cancer, has small toxic and side effects, has the effect of treating solid tumors, and can be used as an anti-tumor polysaccharide drug. # imgabs0 # comprises: m is 1 or 2, and n is an integer between 22 and 617; r1 and R2 are-OH or CH3COO <->.

To provide a skin care preparation which is derived from a natural product, is excellent in biological safety, and exhibits functions of preventing or improving skin roughness, improving the transparency of the skin, and suppressing cell aging by suppressing the secretion of inflammatory substances (cytokine and chemokine) in the skin.SOLUTION: An extract of Gentiana lutea belonging to the genus Gentiana of the family Gentianaceae and an extract of Bletilla striata belonging to the genus Bletilla of the family Orchidaceae are used as active ingredients for suppressing the secretion of inflammatory factor (cytokine and chemokine).SELECTED DRAWING: None

The present invention discloses the coding sequence of the Cymbidium flowering regulation gene CsFTL1 and its application. The Cymbidium flowering regulation protein CsFTL is characterized in that its amino acid sequence is as shown in SEQ ID NO: 2. The present invention isolated the key flower development gene FTL1 from the flower bud cDNA of the Cymbidium sinense variety 'Xiaoxiang' and named it CsFTL1. Temporal and spatial expression pattern analysis found that the expression of CsFTL1 was the highest in the flowers of Cymbidium sinense; among different flower development stages of Cymbidium sinense, the expression trend of CsFTL1 was that the mRNA accumulation increased rapidly at the stage of flower bud differentiation and then gradually decreased. After heterologously expressing CsFTL1 in Arabidopsis thaliana, it was found that Arabidopsis thaliana showed the phenotype of early flowering. The Cymbidium flowering regulation gene of the present invention can be used for the study of the molecular mechanism of flower organ development in Orchidaceae plants and the improvement of plant flowering traits.

The present application relates to the technical field of medicinal plant tissue culture, in particular to a rapid virus-free cloning method and reagent combination for medicinal plant tissue culture. The method comprises the following steps: pretreatment and disinfection of explants, precise virus elimination, preparation of dynamic adaptive medium, rapid propagation, real-time high-throughput detection of virus-free, seedling raising and transplanting, etc. The reagent combination comprises a multifunctional degradable reagent kit, intelligent response type nano disinfection microcapsules, special reagents such as composite virus elimination liquid, etc. The virus elimination rate is greater than or equal to 95%, the survival rate of explants is greater than or equal to 90%, the propagation cycle is shortened by 30%, the detection time is less than or equal to 2 hours, the survival rate of transplanting is greater than or equal to 85%, and the degradation rate of reagents is greater than or equal to 90%. The present application is suitable for herbaceous, woody and liana medicinal plants of the families of Araliaceae, Eucommiaceae, Orchidaceae and Campanulaceae, etc., can realize stable virus elimination and propagation effect, significantly improve the virus-free cloning efficiency and seedling quality, and meet the green production demand.

The invention discloses a flavonol synthase gene CxFLS of impatiens biennis and an application of the flavonol synthase gene CxFLS. A gene with the full length of a gene coding sequence of 1002bp is cloned from'impatiens yunnanensis' for the first time, the gene is a flavonol synthase CxFLS gene, heterologous expression is performed by constructing an overexpression vector, CxFLS is shown to negatively regulate and control the anthocyanin content, and the higher the expression quantity of CxFLS is, the lower the anthocyanin content is and the shallower the flower color is; overexpression of CxFLS in tobacco finds that the flower color of transgenic tobacco becomes light or even white, pure white flower tobacco can be produced, the transgenic tobacco can be used for flower color improvement of ornamental plants, the overexpression CxFLS can be used for constructing light-colored flower plants and rapidly obtaining pure-colored flower materials, the problems that flower color improvement methods such as cross breeding and mutation breeding are long in period and not accurate are solved, and the method is suitable for popularization and application. By regulating CxFLS expression to cultivate varieties with light flower colors, the method has important significance on flower color breeding of ornamental plants.

The present invention discloses a coding sequence for the AP1 flowering regulatory gene in Chinese orchids and its application. This orchid floral organ development regulatory gene was isolated from the floral organ cDNA of the Chinese orchid cultivar 'Bai Mo'. Increasing its expression can cause loss of apical dominance in Arabidopsis thaliana, an increase in lateral inflorescences, irregular arrangement of florets on the inflorescence axis, and abnormal petal development in flowers, with the occurrence of fewer petals or petals transforming into sepals. In Chinese orchids, a significant positive correlation was found between gene expression and an increase in sepals, and expression was significantly elevated in variants with a tree-forked flower pattern, indicating that this gene promotes sepal development in Chinese orchids. The Chinese orchid flowering regulatory gene disclosed herein can be used to study the molecular mechanisms of floral organ development in orchidaceae plants and improve flowering traits in plants.

The application discloses application of gastrodin in preparation of SCA3 disease treatment drugs, and the gastrodin is a compound extracted from Gastrodia elata Blume of the Orchidaceae family and has therapeutic effects on neurasthenia, headache and the like. Researches show that the gastrodin can significantly reduce inclusion bodies caused by mutant ataxin-3, improve soluble ataxin-3 protein levels, has no toxic side effects on HEK293T cells, and regulates the specific molecular mechanism of inclusion bodies formed by ataxin-3 through an AKT / ERK signal axis. The application aims to reveal the application of the gastrodin in playing a neuroprotective effect on SCA3 diseases, enrich new uses of old drugs, and provide a new idea for prevention and treatment of other neurodegenerative diseases.

The present invention discloses a plant fermentation composition for preventing hair loss and promoting hair growth, and a preparation method and application thereof. The plant fermentation composition uses only angelica, Polygonum multiflorum and Platycladus orientalis leaves as plant components. Different from the common direct use of extracts as medicine, the composition of the present invention is firstly water-extracted with angelica, and then treated with enzymes to obtain an enzymatic extract of angelica, and water-extracted with Polygonum multiflorum and Platycladus orientalis, and then treated with enzymes to obtain an enzymatic extract of Polygonum multiflorum and Platycladus orientalis leaves; after the two enzymatic extracts are combined, they are co-fermented with lactic acid bacteria to obtain a plant fermentation composition. After experimental verification, after the three plants are subjected to enzymatic extraction and fermentation composite treatment, the content of the active ingredients in the three plants, namely angelica polysaccharides, diphenylethylene glycosides, and Platycladus orientalis leaf flavonoids, is significantly increased, and there is less precipitation, the stability is significantly improved, and the stickiness is significantly reduced. Compared with the anti-hair loss and hair growth products mixed with general plant extracts, the plant fermentation composition has a better anti-hair loss effect, a higher hair density, and only three plant components, so it is safer.