87 results about "Orchidaceae" patented technology

The invention discloses three fungus strains for promoting the germination of arethusa seeds. The classification names of the three fungus strains are respectively Rhizoctonia sp. Bsg11 with the preservation serial number of CGMCC No.2450, Tulasnella sp. Bsg14 with the preservation serial number of CGMCC No.2451 and Mycena sp. MF24 with the preservation serial number of CGMCC No.1350. The three effective fungus strains have an important meaning for solving the germination problem of arethusa seeds; and the three effective fungus strains can be seeded together with the arethusa seeds, are used for the cultivation of the cultured seedlings or the large fields of a seedling culture disk or a flowerpot or a seedbed and have an application value for replacing tissue culture to obtain seedlings.

The invention discloses a fungus for promoting orchid seeds to germinate. The classified name of the fungus is tulasnellasp. fungus Bsg14 with the preservation number of CGMCC No.2451. The strain has important significance for solving the seed germination problems of dendrobium plants in orchidaceae and bletilla plants, can be sown with the orchid seeds and capable of used for culturing seedling-raising trays, flowerpots or seedbeds or cultivating the land for growing field crops and has application value for obtaining the seedlings instead of tissue culture.

A bowl-type cultivation method for the tropical aerial orchid includes such steps as choosing the culture medium, planting, and applying water and fertilizer. Its advantages are stable and balanced supply of nutrients, high growth speed, and low cost.

The invention discloses an LED light source control system for growth of configuration plants. In the prior art, no light control system of an LED light is directly used for plant growth, particularly tissue culture of orchidaceae plants. The invention adopts a technical scheme that: the LED light source control system for the growth of the configuration plants is characterized by consisting of a control bottom plate, a plurality of control basal plates and a plurality of LED light plates, wherein the LED light plate is provided with an LED light; and the control bottom plate comprises a micro processor and a plurality of power sockets, the micro processor is connected with a computer by a serial port connecting wire, a parameter setting module is arranged in the computer, and the control basal plates are arranged on the power sockets. The system of the invention has the advantages of effectively improving growth amount and effective ingredients of the plants, solving the problems of high energy consumption, long cycle and the like existing in the propagation of the orchidaceae plants and greatly shortening the breeding cycle of seedlings.

The invention discloses dendrobium officinale Kimura et Migo endophytic fungus. The dendrobium officinale Kimura et Migo endophytic fungus is obtained by separating from the living body of dendrobium officinale Kimura et Migo belonging to dendrobium species, orchidaceae by adopting an endophytic fungus separation and purification technology; and identified by the microbial taxonomy, the dendrobium officinale Kimura et Migo endophytic fungus is Pestalotiopsis sp.DO14; the strain is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number CCTCC M 2015180. Through co-culture with dendrobium officinale Kimura et Migo tissue culture seedlings, the steam thickening and lengthening, and the stem and leaf color deepening of the dendrobium officinale Kimura et Migo tissue culture seedlings can be promoted, the improvement of the polysaccharide content in the stem can be promoted, the change of alcohol soluble ingredients in the stem and the flavonoid ingredients in the leaves can be promoted; the dendrobium officinale Kimura et Migo endophytic fungus is an important microbe closely related to the growth and metabolites of dendrobium officinale Kimura et Migo, and has the application potential of promoting the growth of dendrobium officinale Kimura et Migo and the change of the metabolic components.

Paphiopedilum armeniacum, known as 'Golden Child', is one of orchidaceous endangered species in China and has extremely high aesthetic values. Successful and perfect artificial propagation technology has significant importance to the protection and development of paphiopedilum armeniacum. The invention discloses a method which is to introduce tulasnella c alospora into a substrate by using a grown paphiopedilum armeniacum plant and then cultivate aseptic seedlings of paphiopedilum armeniacum in the substrate carrying tulasnella c alospora so as to raise mycorrhizal seedlings of paphiopedilum armeniacum. The invention aims to provide a method for raising mycorrhizal seedlings of paphiopedilum armeniacum with low cost and easy operation to solve the problem of slow growth of cultivated aseptic seedlings. The method has the advantages of readily available tulasnella c alospora resource, easy operation of impregnation, low cost, etc., and has obvious economic and ecological efficiency.

The invention relates to a method for pollination and fructification of orchids in test tubes. In the invention, second filial generation plants are obtained through a process of orchid tissue culture, so the breeding period of the orchids especially Dendrobium candidum is effectively shortened. The breeding period of 4-5 years in a natural state can be shortened to less than one year, and the method of the present invention which has the advantages of short culture time and low cost is in favor of shortening the breeding period of orchid and improving the breeding efficiency.

The invention discloses nutrient soil for orchid plant culture. The nutrient soil is characterized by comprising the following raw materials in part by weight: 20-40 parts of pine bark, 20-40 parts of grass carbon, 20-40 parts of walnut shell, 5-15 parts of vermiculite and 5-15 parts of bamboo carbon. The nutrient soil is suitable for all orchid plants and improvement of disease resistance, and can provide higher-ratio long-acting solid phase organic matters, stable pH value and good water and air control performance. Therefore, the culture management of the orchid plants is more convenient, the root systems of the orchid plants are promoted to grow and then are quickly rejuvenated, the color of orchids is kept, the fragrance of the orchids is stronger, and the orchids are more beautiful. The nutrient soil can be suitable for orchid culture in household balconies and can also be used for industrial large-scale production.

The invention discloses a dendrobium candidum health-care product capable of enhancing human immunity, and belongs to the field of health-care products. The dendrobium candidum health-care product is prepared from the following raw materials in percentage by weight: 25-50% of dendrobium candidum part, 25-45% of coptis chinensis and 25-45% of orange peel, wherein the dendrobium candidum part is dry stem of orchidaceae dendrobium candidum. A preparation method of the dendrobium candidum health-care product comprises the following steps: grinding the dendrobium candidum part by an ultrafine grinding method to obtain raw dendrobium powder; grinding the coptis chinensis by the ultrafine grinding method to obtain raw coptis chinensis powder; grinding the orange peel by an ultrafine grinding method to obtain raw orange peel powder; uniformly mixing the raw dendrobium powder, the raw coptis chinensis powder and the raw orange peel powder to obtain loose powder. The dendrobium candidum health-care product has the characteristics of reducing blood sugar, reducing blood lipid, improving human immunity, refreshing and strengthening stomach.

The invention relates to an orchid seedling growing device. The orchid seedling growing device comprises a support and a water containing groove formed in the support, wherein a supporting pipe is arranged on the bottom of the water containing groove, seedling growing trays are arranged on the top of the supporting pipe, permeable holes and water leakage holes are machined in the bottoms of the seedling growing trays, a filter screen is arranged on each permeable hole, the bottom of each filter screen is connected with a siphon, the lower end of each siphon extends into the water containing groove, each siphon is in the shape of a reversely arranged funnel, and the lengths of the siphons range from the second-grade to the fifth-grade according to the distribution heights and meet the formula L=lambda rga / V. According to the orchid seedling growing device, water in the water containing groove is led into the seedling growing trays according to the siphon principle, nutrients and water can soak into the seedling growing trays from the bottoms of the seedling growing trays, different water requirements of orchids in different growth stages are met, the amount of water in each seedling growing tray is controlled reasonably, water and nutrients are utilized efficiently, the survival rate of the orchids is increased, the problems that in the prior art, the growing difficulty of the orchids is high and the survival rate of the orchids is low are effectively solved, and the orchid seedling growing device is suitable for large-scale orchid seedling growing bases.

The invention belongs to the field of medicine science and technology, and particularly relates to monomer compounds having selective butyrylcholinesterase inhibitory activity obtained by performing separation from orchidaceae Bletilla striata (Thunb)Reichb.f. and medicial salt thereof, and a pharmaceutical composition containing the compounds. The 12 compounds disclosed by the invention have selective inhibitory effects on butyrylcholinesterase to different extents, and IC50 values of five of the 12 compounds are smaller than 10 micrometers, so that a powerful butyrylcholinesterase inhibitoryeffect is shown, and a unique advantage of the compounds in treatment of senile dementia diseases.

The invention discloses a method for inducing endophytic fungi of orchid. The method includes: utilizing a seeding cup to replace a seed bag to perform in-situ symbiosis germination of orchid seed and induce effective endophytic fungi. The method solves the problems about seed clot, low germination rate, proneness to being influenced by weather conditions and the like caused during conventional in-situ symbiosis germination of the orchid. The method is simple to operate, convenient to get materials, low in cost and high in seed emergence rate, seedlings in different growing stages can be selected to perform effective strain separation according to requirements, and a new operable way for protecting endangered orchid and culturing seeding is developed.

The invention discloses shh-53 (Sebacina sp.) promoting germination of plant seed of Dendrobium of Orchidaceae. The shh-53 has important application value on sexual reproduction of plant of Dendrobium of Orchidaceae, large-scale production of seedlings and sustainable utilization of resources.

The invention discloses a method for crossbreeding and aseptic sowing of renanthera coccinea, comprising the following steps of: carrying out hybridization by selecting orchid with obvious characters and high visual value as a hybrid female parent and renanthera coccinea as a male parent; carrying out aseptic sowing on a seed germination medium on the fruit obtained by successful pollination when being basically ripened but not splitted; after germinating, transferring the seeds to a healthy seedling medium; and growing the formed seedling in a test tube out of a bottle after being hardened under the illumination of natural light for 10 to 20 days, wherein the transplanting survival rate of seedling formation can reach 90% or more. The method has the characteristic of high hybrid success rate, and is particularly useful during taking orchid with obvious characters and high visual value as a hybrid male parent which is different from a renanthera coccinea parent in flowering phase. The unique medium and cultivation method are employed by the invention so that the hybrid seeds of renanthera coccinea have high germination, fast seedling formation and good seedling quality. The method has the advantages of strong operation, effective breeding, valuable application and the like.

The invention provides a Coriolopsis strumosa strain for promoting germination of orchidaceae seeds and application thereof, and belongs to the technical field of symbiotic fungi. The strain Epi910 provided by the invention is derived from fungi in the tissue culture germination process of cymbidium seeds, and is identified as Coriolopsis strumosaby combining sequencing comparison results of fragments such as ITS and nLSU of the strain separated from a culture medium and protocorms with morphological characteristics. The strain Epi910 is colonized in seeds and protocorm cells of cymbidium sinense and cymbidium stipule in a mycelium pellet form, and meanwhile, through a symbiotic germination test, it is verified that the Epi910 has similar functions with mucilage film bacteria, and orchid seeds including cymbidium sinense and cymbidium stipule can be promoted to germinate to form seedlings. The symbiotic germination system of the Coriolopsis strumosa-orchid seeds can be used as a new symbiotic mode except for Tulasnella fungi, and a new research object is provided for comprehensively analyzing the symbiotic mechanism of the fungi- orchid plants.

The invention relates to a fungus for promoting germination of seeds of an orchidaceae medicinal plant gymnadenia conopsea. Bacterial colonies of the fungus are rapidly grown in a PDA (Potato DextroseAgar) medium, the bacterial colonies have a great amount of flocculent aerial hyphae, the front sides of the bacterial colonies are white brown, the back sides of the bacterial colonies are light brown, the aged aerial hyphae are black brown, the bacterial colonies are tidy in edge and not uniform in thickness, the growths speed of the hyphae is 0.54-0.57mm / hour, the diameter of the hyphae is 2.71-3.72mu m, a diaphragm structure can be clearly observed microscopically, near right angles can be branched, and sexual reproduction spore or asexual chlamydospore is not generated. The fungus provided by the invention is capable of effectively promoting germination of the seeds of the gymnadenia conopsea, and can be applied to later gymnadenia conopsea seedling breeding.

The invention provides a compound of caulis dendrobii extract and iron and a preparing method and application of the compound. The compound comprises 100 parts of caulis dendrobii polysaccharide and 5-50 parts of iron, and can further comprise 5-20 parts of caulis dendrobii protein; the caulis dendrobii is medicinal caulis dendrobii in orchidaceae dendrobium. The preparing method of the compound includes the steps that a complexing agent is added into a water solution of the caulis dendrobii extract, a FeCl3 solution is added under the alkaline condition to be subjected to a complex reaction, centrifugalization, centrifugal liquid dialyzing and drying are carried out, and the compound is obtained. The compound, prepared with the preparing method, of the caulis dendrobii extract and the iron can be prepared into various dosage forms such as capsules, tablets, oral solutions and granules. According to an iron-deficiency-anemia pharmacological effect experiment and a clinical comparative experiment, the effect better than that of Niferex polysaccharide iron is obtained. The compound can be used for preparing medicine for preventing and treating iron-deficiency anemia, health-care products for preventing and treating iron-deficiency anemia or food additives for preventing and treating iron-deficiency anemia.

Disclosed is an applocation of an abstract of a plant endogenetic fungi fermentation product for antagonizing immunodeficiency virus(HIV). Said fungi is isolated from the roots of Orchidaceae medicinal herb Dendrobium nobile, and is EF1(Epulorhiza sp.); EF1s are fluid fermentation cultured to prepare an abstract of the fermentation product. The invention is provided with simplicity of fermentation art, short period of production, and mass industrialization production. The abstract of the fermentation product is obtained easily, and has a remarkable anti-HIV-1 activity, the acting site of which is after reverse transcription and before HIV RNA expression, with a regulation action of HIV-1 gene expression. An adding action is obtained by a combined use of the said fungi fermentation product and anti-HIV drug AZT, indicated by a drug combination test. The abstract of the fungi fermentation product has an important value in the development of novle anti-HIV drugs, and is hopefule to become a novel anti-HIV drug.

The invention relates to a soil conditioner. The soil conditioner is prepared from, by weight, 100 parts of biogas residues, 40 parts of furfural residues, 100 parts of straw and a proper quantity of a water-retaining agent. A preparation method is provided for acidified soil, soil fertility is enhanced, soil where the soil conditioner is applied enables acidophilous plants such as ericaceae and orchidaceae and other plants to grow well, and garden diversification development is promoted.

The invention provides a traditional Chinese medicine for treating arrhythmia due to heart-spleen deficiency and a preparation method thereof. The traditional Chinese medicine for treating the arrhythmia due to heart-spleen deficiency is prepared from the following raw materials: stropanthus divericatus, lycoris, coriaceous knotweed rhizome, celery, wilson begonia rhizome, fragrant sarcococca herb, sambucus adnata wall, mimosa pudica, corallodiscus flabellate, cinnabar, hairyvein agrimony, grass, orchidaceae ludisia, dioscorea pentaphylla, companumoea root, panicled fameflower root, dillseed, madder root and humulus lupulus. The traditional Chinese medicine for treating the arrhythmia due to heart-spleen deficiency has the beneficial effects that the theory of traditional Chinese medicines is utilized, and the advantages of quick effect, definite therapeutic effect, high safety, no toxic or side effect and difficulty in recurrence can be realized when the traditional Chinese medicine provided by the invention is used for treating the arrhythmia due to heart-spleen deficiency.

This invention discloses a technique for applying MF24 (Mycena sp.) in the culture of protocorm in Dendrobium candidum. The technique comprises: (1) culturing protocorm in Dendrobium candidum and MF24 (Mycena sp.), respectively; (2) manufacturing MF24 (Mycena sp.) into inducer, and adding into protocorm in Dendrobium candidum for co-culture. The cultured protocorm in Dendrobium candidum has higher yield and contents of effective components, and can be used to replace Dendrobium candidum as a pharmaceutical material. The method is simple, and does not need addition of any hormone.

The invention discloses a method for promoting growth of orchidaceae through Ceratobasidium sp.. A symbiotic relationship is established between a strain of ceratobasidium and protocorm / rhizomes and seedlings of the orchidaceae to promote growth of the orchidaceae, wherein the strain of ceratobasidium is Ceratobasidium sp. of ceratobasidiaceae, has the Latin name of Ceratobasidium sp., the GenBankregistration number of MG669582 and the biological preservation number of CGMCC No.15373. Through the promoting function of the Ceratobasidium sp. on growth of the orchidaceae, a foundation is laid for application of the Ceratobasidium sp. in efficient breeding of orchidaceae and resource protection of wild orchidaceae.

The invention relates to an orchidaceae plant three-dimensional multi-layer cultivation device comprising more than two vertical cultivation barrels arranged in a stacking mode from bottom to top and partition frames supported between each two vertically adjacent vertical cultivation barrels. A barrel inner cavity of each vertical cultivation barrel is a compost cavity used for containing culture media, and a barrel body side wall of each vertical cultivation barrel is provided with a seedling planting hole penetrating through the barrel body side wall so that each compost cavity is communicated with the outside. The orchidaceae plant three-dimensional multi-layer cultivation device can timely adjust the height position and the horizontal angle position of planting according to environment requirements such as light, humidity and temperature required by growth of orchidaceae plants such as dendrobium, facilitates planting management such as watering and fertilizing, and is beneficial for improvement of product quality and planting efficiency.

The invention discloses a sterilizing method and a sterilizer for small orchidaceae seeds. By arranging the sterilizer and a relative sterilizing solution and adopting the scientific solution concentration and the sterilizing time, during sterilization, the seeds can be fully and uniformly in contact with the sterilizing solution (the sterilizing solution can uniformly pass through grids in a steel wire net to flow into or out of the sterilizer); meanwhile, during later cultivation, only the steel wire net on a box cover needs to be replaced (namely the mesh number of the steel wire net is reduced and the size of the steel wire net is increased), and the seeds can uniformly pass through the steel wire net to flow into a culture medium so as to finish sterilized cultivation. According to the method, the seeds can be uniformly in contact with a sterilizing agent in the sterilizing process, so that the contact time of the seeds and the sterilizing agent is prolonged; the sterilizing time can be effectively controlled in the sterilizing process; the cracked seeds can be fully utilized, and the seed germination period can be effectively prolonged for non-cracked seeds. Meanwhile, the sterilizer is low in manufacturing cost, convenient to operate, easy to make and reusable.

Provided is a hair growth agent / hair tonic capable of improving the hair generation and growth effects and achieving the anti-canities effects. Specifically, the hair growth agent / hair tonic comprises, as an active ingredient, the extract obtained from the whole part of a plant belonging to the genus Cymbidium of the family Orchidaceae, and also has the anti-canities effect. Great Flower Marie Laurencin is particularly effective as the plant belonging to the genus Cymbidium of the family Orchidaceae. The amount of the extract to be added is 0.1 to 30% by weight, desirably 1.0 to 20% by weight, and particularly preferably 5.0 to 15% by weight.

The invention relates to a liparis nervosa lectin protein obtained by separating and purifying rootstock and leaves of orchidaceae liparis nervosa. The liparis nervosa lectin protein has an amino acid sequence shown as SEQ ID NO:3 in a sequence table. In the invention, the liparis nervosa lectin protein obtained through separation and purification is subjected to determination on an N-end amino acid sequence to judge that the liparis nervosa lectin contains 29 N-end amino acid sequences, a degenerate primer is designed accordingly, a liparis nervosa lectin gene is cloned with 3'RACE and 5'RACE methods, and the nucleotide sequence of the liparis nervosa lectin gene is shown in SEQ ID NO:2. Experiments prove that the liparis nervosa lectin protein has an obvious inhibition effect on agricultural harmful fungi, and can be applied to preparation of pesticides for resisting agricultural harmful fungi and improvement of resistance of plants to the harmful fungi.

The invention discloses a cultivation substrate for dendrobium officinale. The cultivation substrate contains the ingredients in parts by weight: 1-8 parts of pine barks, 1-8 parts of sphagnum moss and 1-8 parts of bamboo charcoal. According to the cultivation substrate, the sphagnum moss has good water retention property and air permeability; the bamboo charcoal brings air permeability and attached weak acidity into full play; and the pine barks are filled and can play a role in fixing the dendrobium officinale. According to the cultivation substrate, the requirements of the dendrobium officinale on characteristics, i.e., air permeability and water retention property of the substrate are met, and problems that diseases and insect pests are propagated, the aging of greenhouse facilities is accelerated, and the like caused by the increase of internal overall ambient humidity of a greenhouse can also be avoided. In addition, the characteristic requirements that Orchidaceae plants like weak acidity are also met.

The invention discloses a planting method for edible orchidaceae plants. The planting method comprises the following steps that 1, a planting greenhouse and a planting bed are erected, the top of the planting greenhouse is covered with a sunshading net, the periphery of the planting greenhouse is provided with removable film, whether the film is removed or not is determined according to the temperature and humidity in the greenhouse, a bed body of the planting bed is provided with a sunshading net, and the sunshading net serves as a bearing body for bearing a planting matrix; 2, the edge of the planting bed is provided with a temperature sensor and a humidity sensor, and the planting bed is connected with an automatic spray device; 3, the planting matrix is laid on the sunshading net according to the growth demands of the edible orchidaceae plants, and the edible orchidaceae plants are planted. The invention further discloses application of the sunshading net in planting of the edible orchidaceae plants by serving as the bearing body of the planting matrix. According to the planting method for the edible orchidaceae plants, the sunshading net which is capable of preventing aging, difficult to decompose, light and convenient is utilized as a material for bearing the planting matrix of the planting bed, the ventilation and breathability effect is good, the obvious effects on increasing the yield and the like are achieved, and the method conforms to the food sanitation standard.

The invention discloses an orchid endangered state estimation method. The method comprises the following steps of: collecting specimen data of orchid target genuses; respectively substituting the specimen data into a Solow equation: P=(tn / T) (n-1), an apparent rate equation: P=(1-(n / tn) (T-tn), and a Robinson and Wheelock equation: P=(tn-tn-1) / (T-tn-1); respectively calculating endangered degrees P of the orchid target genuses; and solving an average value of the three endangered degrees P, wherein the orchids are more endangered if the average value is smaller, n is the number of specimens and is greater than or equal to 3, T is the number of total years from the first recording to the estimation, tn is the number of total years from the last recording to the first recording, and tn-1 is the number of total years from the second last recording to the first recording. According to the method disclosed by the invention, three mathematic models and specimen data in a herbarium are utilized to estimate the endangered states of the orchids; and the adopted mathematic models consider the starting time and ending time of the specimen data and the acquisition frequencies of the specimens, so that the method has relatively strong scientificity and adaptability, and can provide basis for the final estimation of the endangered states of the orchids.

The invention relates to an export quarantine treatment method and an export quarantine treatment device for fresh orchidaceae cutflowers. The method comprises the following steps: placing the cutflowers to be treated, which are placed on a flower shelf, in a treatment bin; closing a bin door and air inlets; starting an air engine, adjusting pressure and reading a pressure value and a flow value; starting an agricultural agent storage device, reading a pressure value and a flow value; adjusting technical parameters of a two-fluid control system, such as liquid pressure, air pressure, flow rate, treatment time to reach technical coverage; monitoring operation conditions in the treatment bin; stopping the agricultural agent storage device; stopping an air source when a reading value is zero; and switching off a power source and a relative pipeline. The export quarantine treatment device comprises a distribution system, an infusion and air-transmission pipeline of the two-fluid control system, a two-fluid atomizing nozzle, the treatment bin and an exhaust system. The export quarantine treatment method for the fresh orchidaceae cutflowers is economic, safe and convenient, has little influence on the environment, can effectively improve the quarantine treatment speed and the treatment efficiency, achieves the export quarantine requirements without influencing the cutflower preservation effect, reduces manpower input and decreases the production cost.