527 results about "Inflorescence" patented technology

The invention discloses a phoebe bournei PbGRF1 gene, and the phoebe bournei PbGRF1 gene has a nucleotide sequence as shown in SEQ ID No: 1, or a sequence which is obtained by deleting, adding and / or substituting one or more nucleotides of the sequence and has an unchanged function. The invention also discloses a protein coded by the phoebe bournei PbGRF1 gene. The invention further discloses a method for promoting plant leaf growth. The invention also discloses an application of the phoebe bournei PbGRF1 gene in promoting the growth of plant leaves. The phoebe bournei PbGRF1 gene is cloned, arabidopsis thaliana is transformed through an agrobacterium tumefaciens mediated inflorescence infection method, a phoebe bournei PbGRF1 gene overexpressed plant is obtained, phenotypic observation shows that the leaf area of the arabidopsis thaliana with the overexpressed phoebe bournei PbGRF1 gene is larger than that of a wild type Col, and meanwhile the fresh weight and the dry weight of the overexpressed plant are larger than that of the wild type Col.

The invention relates to the technical field of pineapple planting, in particular to a management and control method for reducing malformed fruits caused by non-development of pineapple kernels. The management and control method comprises the steps of regulating and controlling nutrition before a flowering period, optimizing a microenvironment in a flowering period, strengthening management in a young fruit period and comprehensively preventing and controlling diseases and insect pests. The operation of regulating and controlling the nutrition before the flowering stage comprises the following steps: supplying a water-dispersible slow-release fertilizer by adopting a drip irrigation system from a seedling stage to a flowering accelerating stage; the mass ratio of nitrogen to phosphorus to potassium in the water dispersible slow-release fertilizer is 1: (1.1-1.3): (1.4-1.6); the operation of optimizing the microenvironment in the flower forcing period comprises the following steps: forcing flowers in cloudy days at the temperature of 18-25 DEG C or at dusk; after the inflorescence develops, a mixed solution of ethephon and gibberellic acid is adopted for core filling; the operation of strengthening young fruit stage management comprises the following steps: in a young fruit stage, spraying a mixed aqueous solution of sugar alcohol calcium and monopotassium phosphate to leaf surfaces; the operation of comprehensively preventing and controlling diseases and insect pests comprises the following steps: covering with breathable insect-preventing fruit bags 20 days after flowers wither, and releasing natural enemies to control the cardinal number of pests.

The invention discloses a vitis amurensis calcium-binding protein gene VamCP1 as well as an encoding protein, an expression vector and application of the vitis amurensis calcium-binding protein gene VamCP1. According to the application, a pCAMBIA2300-VamCP1-GFP overexpression vector is constructed, and is respectively transformed into arabidopsis thaliana and 'Chardonnay' grapes through an agrobacterium tumefaciens-mediated inflorescence infection method and a transient transformation method, and the resistance of a transgenic plant of the calcium binding protein VamCP1 under low-temperature stress is researched. The result shows that the VamCP1 gene can improve the activity of antioxidant enzyme and the content of osmoregulation substances, reduce the damage of plant cell membranes and reduce the permeation of electrolyte. The gene enhances the low-temperature stress resistance of plants by inducing the expression of related cold-resistant genes and eliminating excessive ROS.

The invention relates to the field of plant cultivation, and particularly discloses a lifting type high-density strawberry annual production method capable of breeding seedlings in spring and summer and fruiting in autumn and winter, which comprises the following steps: planting strawberry stock plants on a lifting type high rack after cold demand accumulation, and carrying out long-day high-temperature treatment to promote stolons to grow; cutting the seedlings formed on the stolons into a hole tray placed on a high rack in a pressure drawing manner, cultivating until the root system is robust, and cutting off the stolons; the obtained child seedlings are new seedlings, and the mother plants are old seedlings; and processing the new seedlings and the old seedlings at low temperature and short day in autumn and winter so as to enable the new seedlings and the old seedlings to bud and bear fruits in autumn and winter of the current year, and harvesting the strawberries mature in the next year. According to the method, strawberry stock plants are subjected to low-temperature refrigeration treatment before field planting, it is ensured that stolons are bred in spring and summer, plug seedlings needed by production are cultivated, meanwhile, the stock plants are kept to continue to bear fruits, tillering increase, inflorescence increase and fruit increase are promoted due to the fact that a large number of stolons grow, and the high-yield effect is achieved.

The invention discloses a gene ZmER1 coded by a corn receptor kinase. The CDS (Coding Sequence) of the gene is shown as SEQ ID No.1. The invention also discloses a preparation method of the gene ZmER1. The CDS nucleotide sequence of the gene mutant Zmer1-I1 is as shown in SEQ ID No.3, the amino acid sequence of the gene is as shown in SEQ ID No.4, the CDS nucleotide sequence of the mutant Zmer1-D8 is as shown in SEQ ID No.5, and the amino acid sequence of the gene is as shown in SEQ ID No.6. The invention further discloses a preparation method of the gene. The invention also discloses phenotypes in the aspects of reducing the height of stem tip meristem and increasing the size of female ear inflorescence meristem after mutation of the receptor kinase coded gene ZmER1, which indicate that the ZmER1 gene has a good application prospect in the aspects of cultivating a new semi-dwarf variety resistant to close planting and increasing the row number of corn ears.

The invention belongs to the field of plant molecular biology, and particularly relates to an EjTGA1 gene related to loquat flowering phase and growth development regulation, a protein coded by the EjTGA1 gene and application of the EjTGA1 gene. The full length of the cDNA sequence of the gene is as shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein of the gene is as shown in SEQ ID NO.2. The EjTGA1 gene disclosed by the invention is predominantly expressed in the loquat flower. An expression vector pCambia2300-EjTGA1-eGFP is introduced into wild type arabidopsis thaliana through an agrobacterium tumefaciens-mediated inflorescence infection method, the overexpression of transgenic arabidopsis thaliana 35S:: EjTGA1 promotes the arabidopsis thaliana to bloom in advance, and the leaf area and the rosette leaf number are increased. The transgenic arabidopsis thaliana plant material obtained by using the loquat EjTGA1 gene can advance flowering and fruiting time and regulate growth and development, and has a good application prospect.

The present invention belongs to the technical field of plant pollination devices, and particularly relates to a handheld electric fruit tree pollinator and a pollination method. The handheld electric fruit tree pollinator includes: a depth camera (1), a lateral vibration motor (2), a lateral vibration motor cover (3), a solenoid valve (4), a solenoid valve cover (5), a sealing ring (6), a filter screen (7), a filter screen connecting piece (8), a fluff brush connecting piece (9), a fluff brush (10), a longitudinal vibration motor cover (11), a longitudinal vibration motor (12), a powder chamber cover (13), a powder chamber (14), an elastic rod (15), a screw (16), a multi-joint arm hand rod (17), and an intelligent controller (18); the present invention is convenient to carry and simple to operate; the solenoid valve is closed in the non-working state, reducing pollen waste; based on the pollination principle of insects (bees), the pollen is charged with static electricity through the fluff brush and high-frequency vibration, improving the pollination success rate and reducing the damage to the inflorescence.

The invention discloses a cone inflorescence type fruit tree flower three-dimensional reconstruction male and female flower counting method and system, and the method comprises the steps: S1, collecting an image sequence, shooting an inflorescence video through a camera, and extracting the video into an image frame by frame; s2, image preprocessing and camera parameter estimation: preprocessing the inflorescence image, and acquiring a camera parameter corresponding to each image by using a COLMAP tool; s3, a D-NeRF model is trained, and multi-modal three-dimensional reconstruction of the inflorescence point cloud is carried out by using the D-NeRF model; s4, the three-dimensional point cloud output by the D-NeRF model is optimized and marked; s5, training a PointNet + + model, and performing male and female flower identification and counting by using the PointNet + + model; and S6, dividing a data set, and adopting a Bayesian optimization algorithm to adjust and optimize key hyper-parameters in the training process of the D-NeRF model and the PointNet + + model. According to the invention, through integration of NeRF reconstruction, point cloud processing, deep learning target detection and mobile terminal deployment, three-dimensional reconstruction of fruit tree inflorescence and male and female flower identification and counting are realized.

The invention belongs to the technical field of rape breeding and molecular biology, and discloses a molecular marker related to main inflorescence pod density (SDMI) and application of the molecular marker, the sequence of a forward primer for amplifying the molecular marker is 5 '-CCTAACCACCTACGCTGAG TTATATATAGATT-3', and the sequence of a reverse primer for amplifying the molecular marker is 5 '-TAG TCTGTAAATTTATTATGAGCTTCCTTGTCTT-3'. The total DNA (deoxyribonucleic acid) of a single rape plant is used as a template, and PCR (polymerase chain reaction) amplification is carried out by using the primer sequence, so that two bands of 279-bp and 254-bp can be obtained; the rape single plant only having a 279-bp single band is a sparse fruit individual, and the rape single plant only having a 254-bp specific band is a dense fruit individual. The molecular marker developed by the invention can be used for identifying and predicting the pod density, the operation is simple and convenient, the method is easy to implement, the offspring selection is more targeted, and the selection efficiency is improved.

The invention discloses a China rose inflorescence whole genome SNP molecular marker combination and application thereof. A traditional breeding method for improving China rose inflorescence characters is time-consuming and labor-consuming, and ideal plants are difficult to obtain quickly. The SNP molecular marker technology is utilized, 358 Chinese rose materials and inflorescence phenotype data are analyzed through whole genome association, 1574 SNP loci remarkably related to inflorescence are screened out, a whole genome selection model with the prediction accuracy larger than 0.743 is constructed, and inflorescence characters can be effectively predicted. Meanwhile, a corresponding probe is synthesized to be used for capturing and sequencing, the capturing efficiency is high, the coverage depth is good, the SNP sites related to the rosa chinensis inflorescence can be accurately detected, an efficient technical means is provided for rosa chinensis genetic improvement and new variety cultivation, and the development of the rosa chinensis industry is assisted.

The invention provides a brassica napus BnaC3. LEAFY early blossoming molecular marker and application thereof in breeding, and belongs to the technical field of plant molecular breeding and gene editing. Aiming at gene structure variation analysis of a large fragment on a genome of a brassica napus core germplasm resource, genetic loci influencing inflorescence formation are excavated, and the early blossoming molecular marker is used for early blossoming of the brassica napus BnaC3. LEAFY early blossoming of the brassica napus BnaC3. LEAFY early blossoming. And a corresponding gene structure variation sequence DEL00110133 is obtained. A molecular marker is developed on the basis of the structural variation sequence, and the obtained molecular marker can be used for screening germplasm with the characters of early inflorescence establishment and early flowering time in different germplasm resources of the cabbage type rape, so that subsequent rapid breeding of the rape is facilitated. According to the invention, CRISPR / Cas9-based genetic sequence editing is carried out on the gene structure variation sequence to obtain a new late flowering genetic sequence, and a new method and a new gene sequence resource are provided for breeding of brassica napus with late inflorescence establishment and late flowering time.

The present invention provides a BnaC3.LEAFY early flowering molecular marker of Brassica napus and its application in breeding, belonging to the field of plant molecular breeding and gene editing technology, and analyzes the gene structure variation of large fragments on the genome of the core germplasm resources of Brassica napus, excavates the genetic loci that affect the early and late formation of inflorescence, and obtains the corresponding gene structure variation sequence DEL00110133; molecular markers are developed based on the structure variation sequence, and the obtained molecular markers can be used to screen the germplasm with early inflorescence establishment and early flowering time traits in different germplasm resources of Brassica napus, which is beneficial to the subsequent rapid breeding of rapeseed. The present invention also performs genetic sequence editing based on CRISPR / Cas9 on the gene structure variation sequence to obtain a new late flowering genetic sequence, and provides a new method and new gene sequence resources for the breeding of Brassica napus with late inflorescence establishment and late flowering time.

The invention discloses a nondestructive testing method and system for pesticide residues of field drinking chrysanthemum, and the method comprises the steps: obtaining hyperspectral reflectivity data of leaves of the drinking chrysanthemum in a key growth period after pesticide drip irrigation, and carrying out data preprocessing; a quantitative estimation model of the leaf hyperspectral reflectivity and the leaf clothianidin actually measured content is constructed through statistical modeling; determining a regression model equation of the clothianidin content in the leaves and inflorescence by using actually measured data, and calculating the inflorescence residue level according to a leaf detection result; a drinking chrysanthemum clothianidin residue early warning threshold value is set, the hyperspectral reflectivity of drinking chrysanthemum leaves is collected through hyperspectral equipment and input into the model, and drinking chrysanthemum clothianidin residue early warning is achieved; according to the method, early warning of pesticide residues is achieved before chrysanthemum harvesting, especially field rapid screening and quantitative analysis of novel pesticides such as clothianidin are achieved, the technical blank of an existing hyperspectral technology in pesticide residue detection and application of medicinal plants is filled up, and the chrysanthemum quality safety guarantee level is improved.

The invention discloses an inflorescence gene silencing method which comprises the following steps: firstly, synthesizing a specific nucleotide fragment according to a target gene, and constructing the specific nucleotide fragment to a plant virus vector; introducing the constructed plant virus vector and the corresponding helper plasmid into the inflorescence; soaking the inflorescence in a metal chelating agent salt solution; and finally, screening to obtain plants with inflorescences subjected to target gene silencing. The chrysanthemum capitulum is selected as an infection object, the silencing method is optimized, the target gene silencing efficiency is remarkably improved, the phenotype detection period after infection is remarkably shortened, and the method is efficient, convenient and high in operability. Meanwhile, the method can effectively avoid the cross protection phenomenon of plants, overcomes the obstacle of limited transmission range of viral vectors, does not need to consider the problems of death and growth cycle of tissue culture seedlings or seedlings after infection, and provides a feasible means for rapid identification of chrysanthemum gene functions and large-scale development of chrysanthemum gene function research.

The invention provides a papaya CpMp gene, a protein, a primer pair, a recombinant expression vector and application, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the papaya CpMp gene is as shown in SEQ ID No. 1. The papaya CpMp gene has the effect of regulating and controlling the length of inflorescence stalks. According to the present invention, by using the difference of the CpMp gene on the Y chromosome and the Yh chromosome, i.e., the 4691bp transposon insertion exists on the CpMp promoter on the Yh chromosome, and the transposon insertion does not exist on the CpMp on the Y chromosome, the CpMp gene is adopted as the molecular marker to distinguish the male plant from the male-female plant, and the difference can be used in the early sex verification work of the papaya;

The invention discloses a cultivation method for controlling tips and promoting flower bud differentiation of syzygium samarangense, and belongs to the technical field of tip control cultivation of syzygium samarangense. The method comprises the following steps: slightly shearing 70-80 days before flower forcing in the wax apple production period, applying a phosphorus-potassium-silicon mixed fertilizer in holes on the ground, and applying the fertilizer once every 15 days, totally twice; then a mixed aqueous solution of potassium phosphite and paclobutrazol is sprayed to leaf surfaces and drips water, spraying is conducted once every 15 days, and spraying is conducted 2-3 times in total; after spraying is conducted for 5-7 days, closed girdling is conducted, after 40-45 days, a mixed aqueous solution of auxin and a plant nutrition regulator is used for spraying, spraying is conducted once every 15 days, and spraying is conducted 1-2 times in total. The silicon fertilizer is mixed and added in the fertilizer and water management period to assist in increasing the absorption and utilization rate of the phosphorus-potassium fertilizer; besides, through the synergistic effect of tip control, trunk girdling and a mixed aqueous solution of auxin and a plant nutrition regulator, syzygium samarangense plants are stable, flower bud differentiation is promoted, meanwhile, the method has the characteristics of disease resistance, disease prevention, high flower forcing and flowering rate and large inflorescence amount, and the important effects of annual flowering and fruiting and promotion of syzygium samarangense industry synergy can be achieved; the method has the advantages of simple operation process, high efficiency, stability, few use times and labor cost saving.

The new and distinct hardy perennial plant, Heliopsis ‘Touch of Blush’, has dense, rounded, mound habit with extensively-branched, predominately strong upright stems that resist lodging. The foliage is narrowly ovate to broadly lanceolate with green surrounding the veins and near white between the veins and the young foliage shows a reddish-purple blush. Inflorescences have a single layer of overlapping, golden-yellow ray florets and dark-brownish central disk florets. The new plant is freely flowering and begins blooming in early summer and continues for about six weeks. The new plant is useful in the landscape en masse, as an accent, in containers, or as cut flowers.

A new cultivar of Rudbeckia plant named ‘RUDBSS851’ that is characterized by its ng and sturdy stems, its compact plant habit, its flowering that starts early in the season, its inflorescences with numerous ray florets covering the flower, disc florets are present but not visible as they are fully covered by the ray florets, its ray floret colors that are yellow-orange with the lower half greyed-purple and the tip tinged greyed-red, and its large flowers.

This invention relates to a new and distinct variety of strawberry plant named ‘BG-15.490.061’. This new strawberry plant named ‘BG-15.490.061’ is primarily adapted to the growing conditions of the central coast of California, and is primarily characterized by its orange red to red fruit color, medium fruit size, conical fruit shape, with slight differences between primaries and secondaries; fair fruit flavor, with seeds typically held even the surface; medium to large plant size, with medium plant density; medium yellow green foliage color, with slightly concave to flat cross section; and fruiting trusses typically held even to below the plant, with little to no anthocyanins and weak pubescence.

The present invention relates to a method for plant genome modification of at least one plant cell being in the developmental stage of a plant immature inflorescence meristem (IIM) cell, wherein the modification of the specific cell type is achieved by providing a genome modification or editing system, optionally together with at least one regeneration booster, preferably wherein the effector molecules are introduced by particle bombardment. To this end, new artificial and precisely controllable booster genes and proteins are provided. Further, the modified plant cells are regenerated in a direct or an indirect way. Finally, methods, tools, constructs and strategies are provided to effectively modify at least one genomic target site in a plant cell, to obtain the modified plant cell and to regenerate a plant tissue, organ, plant or seed from the modified plant cell.

A new and distinct cultivar of Phalaenopsis plant named ‘Carajillo’, characterized by its upright plant habit; moderately vigorous growth habit; strong flowering stems; strong leaves; freely flowering habit with typically two inflorescences developing per plant, each inflorescence with numerous flowers; flowers with purplish pink-colored petals with a few fine purplish red-colored dots towards the base; and good postproduction longevity.

The present invention provides a mango embryo-promoting agent and an embryo-promoting method, belonging to the field of plant planting technology. The mango embryo-promoting agent includes an inflorescence development stage embryo-promoting solution I; the formula of the inflorescence development stage embryo-promoting solution I includes: MS culture solution, AVG, GA3, imidacloprid, spinetoram, 6-BA, and KH2PO4; the mango embryo-promoting method comprises applying the mango embryo-promoting agent during the mango planting process to promote mango embryo development. The mango embryo-promoting agent and the embryo-promoting method of the present invention can reduce the proportion of mango embryoless fruits to about 80%, a reduction of about 10%.

The present invention relates to the field of biotechnology and relates to a kind of alfalfa MsCAL Gene, and its application, MsCAL The nucleotide sequence of the gene is shown in SEQ ID No.29. MsCAL The gene is specifically expressed in the plant stem apex, with the highest expression level in the floral organs during the reproductive growth stage, indicating that it is specifically involved in the plant flowering process. MsCAL Gene overexpression caused transgenic Arabidopsis to flower early, become dwarfed, and have fewer rosette leaves. In addition, the transgenic Arabidopsis changed from an indeterminate inflorescence to a finite inflorescence. Quantitative results of known genes regulating flowering time and floral organ development in Arabidopsis showed that MsCAL It can regulate the expression of downstream flowering and flower organ development related genes. MsCAL The expression delayed flowering, reduced crude fiber and lignin content, and increased forage digestibility and crude protein content.

The invention discloses a physical induction method of poplar 2n pollen. The invention uses low temperature as an inducer to induce and treat poplar pollen mother cells to obtain poplar 2n pollen; further, by optimizing the technical conditions for inducing chromosome doubling of poplar pollen by low temperature treatment, the optimal technical parameters such as effective treatment period, treatment temperature and continuous treatment time are screened, and finally high-frequency poplar 2n pollen is obtained, and a technical system for low-temperature induction of poplar pollen chromosome doubling and breeding triploids is established, which greatly improves the ratio of poplar 2n pollen, and the male inflorescence of poplar treated by low temperature induction can shed pollen normally and has high pollen vitality, which is suitable for hybrid pollination of triploids; compared with the current colchicine induction and high temperature induction methods, the low temperature induction of 2n pollen has the advantages of simple operation, low cost, high relative safety, etc., and has theoretical and practical significance in constructing a new and efficient poplar triploid breeding technology system.

This invention relates to the field of fruit tree pollination equipment, specifically to an integrated device for precise pollination of pear tree inflorescences and constant temperature maintenance of pollen activity. The device includes a guide tube, with a constant temperature tube sleeved outside the guide tube and fixedly connected to it. One end of the guide tube has a gas path switching component for switching gas flow direction. The gas path switching component includes a switching cavity fixedly connected to one end of the guide tube, with several air inlets on the sidewall of the switching cavity. The end of the switching cavity away from the guide tube is fixedly connected to the inner sidewall of the constant temperature tube, and the end of the constant temperature tube away from the switching cavity has several exhaust holes. A T-shaped slider is slidably fitted onto the inner sidewall of the switching cavity. This invention, through the gas path switching component and the telescopic guide hood assembly, solves the problems of poor pollen activity maintenance due to large temperature fluctuations before spraying and during transport in existing pear tree inflorescence pollination processes, as well as inaccurate pollination caused by external wind disturbances during pollination.

A new and distinct cultivar of Bidens plant named ‘DPWBIDCAPR’, characterized by its upright to outwardly spreading and uniformly mounding to semi-trailing plant habit; moderately vigorous to vigorous growth habit; freely branching habit; dense and bushy plant form; freely flowering habit; long flowering period; single-type inflorescences; inflorescences with solid dark red-colored ray florets that with subsequent development become dark reddish orange (distally) and yellow (proximally) bi-colored; and good garden performance.

A new and distinct cultivar of Gerbera plant named ‘GOS202117’, characterized by its broadly upright and uniformly mounding plant habit; moderately vigorous to vigorous growth habit and moderate growth rate; dense and bushy appearance; numerous inflorescences with bright red-colored ray florets; upright and moderately strong peduncles; and good garden performance and relative tolerance to low temperatures.

The invention discloses application of soybean GmABCG5L gene in improving plant seed setting rate, and belongs to the technical field of plant breeding. In order to solve the problem of how to promote plants to improve seed setting rate, the invention provides a method for promoting plants to improve seed setting rate, wherein a recombinant vector is obtained by constructing a GmABCG5L gene and connecting it with a pROK vector, the recombinant vector is transferred into Agrobacterium to prepare Agrobacterium carrying the GmABCG5L gene, and then a transgenic plant carrying the GmABCG5L gene is obtained by using an Agrobacterium-mediated leaf disc method, and by culturing the transgenic plant carrying the GmABCG5L gene, it is found that the transgenic plant carrying the GmABCG5L gene has a good growth state, an enlarged inflorescence compared with a wild type, an increased number of florets, and a significantly improved total seed amount compared with a wild type, indicating that the GmABCG5L gene can improve the plant seed setting rate.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a PoPILS1a gene in regulation and control of plant seed development. The coding sequence of the PoPILS1a gene is as shown in SEQ ID NO: 1. According to the invention, the specific expression of the peony PoPILS1a in seed tissues is found for the first time, the expression levels are greatly different in different development stages, and the PoPILS1a protein is positioned on a cell endoplasmic reticulum. In an arabidopsis thaliana positive strain with over-expression of PILS1a, the epiphytic point between pods is obviously shortened, the number of pods with limited inflorescence length distribution is larger, meanwhile, the lengths and the weights of the pods at the corresponding positions are obviously different compared with those of a contrast, and it is clear that PILS1a can promote development of fruits and has good practical application value.

The new and distinct hardy perennial Heliopsis plant named ‘Rays for Days’ with dense, rounded, extensively-branched habit, strong, tall, upright stems that resist lodging. The foliage is medium green and broadly ovate to narrowly deltoid. Freely-flowering double inflorescences in a capitulum with multiple rows of golden-yellow, overlapping, ray florets beginning in early July and continuing for about 6 weeks. The new plant is useful in the landscape en masse, as an accent, in containers or as cut flowers.