82results about How to "Regulate growth and development" patented technology

The invention discloses modified polyurethane suspended filler which comprises, by weight, 10 parts to 80 parts of polyhydroxyalkanoate, 10 parts to 80 parts of activated carbon, 60 parts to 80 parts of polyurethane, 1 part to 10 parts of water-soluble polyurethane solutions, 0 part to 10 parts of bioactivator, 0 part to 1 part of inorganic metal salt, 0 part to 20 parts of organic high-molecular polymer and 0 part to 10 parts of inorganic flocculate. A preparation method of the modified polyurethane suspended filler includes the steps of taking the 10 parts to 80 parts of polyhydroxyalkanoate, the 10 parts to 80 parts of activated carbon, the 0 part to 10 parts of bioactivator, the 0 part to 1 part of inorganic metal salt, the 0 part to 20 parts of organic high-molecular polymer and the 0 part to 10 parts of inorganic flocculate, dissolving the mixture in the 1 part to 10 parts of water-soluble polyurethane solutions, soaking the 60 parts to 80 parts of polyurethane in the solution to obtain the loaded polyurethane suspended filler, and conducting secondary drying after primary drying is conducted to obtain the modified polyurethane suspended filler. The modified polyurethane suspended filler has the advantages of being high in porosity and large in specific area.

A fruit-taste yellow wine is prepared from fruit chosen from carambola, haw, jujube, litchi, apple, pear, etc. glutinous rice and rice leaven through choosing raw materials, culturing fruit-taste yeast, saccharifying, and fermenting twice. Its advantages are good color, taste and smell, and rice nutrients.

The invention discloses a wood-vinegar-containing biological compound fertilizer. The biological compound fertilizer is mainly prepared from the following raw materials in parts by weight: 10-15 parts of microbial inoculant, 20-30 parts of urea, 5-10 parts of diammonium phosphate, 10-20 parts of potassium chloride, 4-8 parts of calcium magnesia phosphate fertilizer, 10-15 parts of biochar, 1-3 parts of nano titanium dioxide, 0.5-1.5 parts of zinc sulfate, 0.2-1 part of copper sulfate and 2-5 parts of wood vinegar. The biological compound fertilizer is capable of enhancing the utilization ratio of the fertilizer, saving the raw materials and increasing the crop yield, and is also capable of supplying comprehensive nutrients required by crop growth, regulating plant growth and development, killing and resisting bacteria, expelling pests, effectively lowering the disease and pest morbidity and reducing the pesticide use.

The invention relates to the field of biostimulants and particularly relates to a preparation method for a plant growth stimulant and an application of the plant growth stimulant. The plant growth stimulant is prepared through preparing a seed solution from yellow-ocher streptomyces, inoculating the seed solution to a fermentation tank containing a mixed culture medium of maize meal, soybean meal, glutamic acid, potassium dihydrogen phosphate, sodium chloride, seaweed powder and cerealose which are in a certain ratio, carrying out fermentation, and uniformly mixing fermentation broth of the yellow-ocher streptomyces with multivitamins, multiple amino acids and algal polysaccharides which are in a certain ratio. The plant growth stimulant disclosed by the invention can be added into organic fertilizers, water-soluble fertilizers and microbicides according to a certain ratio so as to carry out synergizing, increase yield, prolong drug effect duration and enhance immunity. The plant growth stimulant disclosed by the invention has the effect of playing a role in stimulating natural courses of plants, including being beneficial to the enhancement of nutrient absorption, nutritional effects, abiotic stress resistance and crop quality, promoting the growth of roots, stems and leaves of crops, and improving the resistance of the crops, can be used for stimulating cell division of plants, promoting formation of chlorophyll and enhancing photosynthesis of the plants and has the aims of promoting growth, advancing ripeness, keeping enough flowers and young fruits, increasing yield, improving crop quality and achieving the yield increase and high yield of the crops.

The invention discloses multifunctional pig feed and belongs to the technical field of livestock feeding. The multifunctional pig feed comprises, by mass, corn, fermented soybean meal, traditional Chinese medicine residues, algae, bran, fish meal, soybean oil, calcium hydrophosphate, stone powder, compound enzymes, lysine, citric acid, L-methionine, threonine, tryptophan, vitamin premix, choline, organic trace element premix, bacillus subtilis, feeding probiotics, compound enzyme preparation, ethoxy quinoline, calcium propionate, oregano oil, an anti-stress agent and a physiological regulation agent. By the specific formula and the specific proportion, the multifunctional pig feed can effectively promote pig growth, increase lean meat yield, increase pig growth immunity and increase the economic benefits of pig raising.

The phytological and agronomic application of angustmycin includes delaying senility of plant leaf and preserving fruits and vegetables along or together with other components, inducing plant organic to dedifferentiate to form callus, activating and maintaining meristic capacity of plant cells, inducing callus to further dedifferentiate root, bud, tuber and other organs, promoting plant seed germination and seedling growth and regulating growth of plant. Owing to the multiple bioactivity, easy dissolution in water, stable performance, easy use and low cost, angustmycin may become new phytokinin with wide application prospect.

A plant growth regulator 'Baoguo effervescent tablet' for regulating the growth and development, promoting the fruit bearing, improving fruit quality and increasing the yield of fruit tree is prepared from gibberellins, forchlorfenuron, potassium dihydrogen phosphate, boric acid and zinc sulfate through proportional mixing, adding citric acid, sodium dicarbonate, adhesive and surfactant, pulverizing, granulating and die pressing.

The invention discloses a soybean biological seed coating agent which is prepared from the following components in percentage by weight: 20 percent of complex microbial community, 5 percent of bio-regulator SODM, 10 percent of trace element, 10 percent of auxiliary agent, 0.4 percent of total matrines, and the balance of water. The invention has the advantages of environmental protection, enhancement of crop stress tolerance, strengthening of seedling, and improvement of yield and quality.

The invention belongs to the field of cell engineering technology, relates to a minisize China rose induction method, and discloses an efficient rapid minisize China rose bud induction method including obtaining of sterile seedlings, China rose bud induction culture, flower culture and other steps. According to the method, pot cultured minisize China rose is used as a plant material, in a stem segment in vitro regeneration system, a most suitable medium for minisize China rose blossoming induction is finally determined as MS + TDZ1.0mg / L + saccharose 40g / L + agar 7.2g / L, test-tube plantlet blossoming is successfully induced, the blossoming rate is increased to 87.1%, a minisize China rose test-tube plantlet little in posture and beautiful and bright in color is successfully cultivated, and the method has important theoretical and practical significance to the China rose asexual breeding.

The invention discloses a novel plant growth modifier. The modifier is prepared from the following raw materials in parts by weight: 0.1 to 0.7 part of phytocytomine, 15 to 28 parts of tea saponin, 4to 8 parts of medical stone meal, 17 to 22 parts of forchlorfenuron, 1 to 4 parts of gibberellin, 60 to 75 parts of chitosan, 18 to 27 parts of humic acid, 8 to 16 parts of cortex meliae, 9 to 17 parts of radix sophorae flavescentis, 10 to 14 parts of diethyl aminoethyl hexanoate, 5 to 12 parts of brassinolide, and 6 to 11 parts of auxin. Compared with the prior art, the novel plant growth modifier disclosed by the invention has the advantages that the compatibility is scientific, a growth development process of crops can be effectively regulated, and the effects of stabilizing and increasingproduction, improving quality, enhancing resistance of the crops and the like can be achieved; moreover, due to the chitosan, an immune system of plants can be stimulated, defense reaction is activated, disease-resistant active substances of the plants are promoted so as to inhibit the diseases and the growth of the plants is promoted. In addition, traditional Chinese medicine components are addedin the plant growth modifier, so that the plant growth modifier has the effects on sterilization, prevention of plant diseases and insect pests and the like, and the use of pesticides such as a bactericide is reduced.

The invention discloses an application of an RSPO3 gene in sow ovarian granulosa cells. Two kinds of follicles with diameters of 3-5 mm and 7-10 mm respectively are used as experimental materials, andthe relationship between H3K4me3 and the expression level of genes related to ovarian follicle development is studied by a sequencing technology. The enrichment degree of H3K4me3 in a promoter regionof the RSPO3 gene is found to be significantly different in the follicles with different size by ChIP-Seq; the expression quantity of the RSPO3 gene in the follicles with different size is found to be significantly different by qRT-PCR; promotion of the degree of the H3K4me3 is found to promote transcription of the RSPO3 gene by promoting or inhibiting the degree of H3K4me3 in the sow ovarian granulosa cells, and inhibition of the degree of H3K4me3 is found to inhibit transcription of the RSPO3 gene; the RSPO3 is found to promote the proliferation of the sow ovarian granulosa cells and inhibit the apoptosis of the cells through overexpression or interference of RSPO3.

The invention relates to a new biological material for preventing and controlling plants from plant diseases and insect pests, particularly to a preparation method of a biological pesticide using a new biological material amino polysaccharide ester as a main component, and belongs to the technical field of biological pesticides and biochemistry. According to the present invention, the new biological material with advantages of efficient prevention and control of plant diseases and insect pests of crops and crop growth promoting is prepared through the biological reaction action of microorganisms or biological enzymes, and the positive significance is provided for the reduction of the dependence of agricultural production on chemical pesticides.

The invention discloses a fertilizer for improving the stress resistance of corn. Raw materials of the fertilizer comprise pig manure, marsh mud, straw powder, fly ash, cake meal, urea formaldehyde, calcium nitrate, a calcium magnesium phosphate fertilizer, potassium dihydrogen phosphate, potassium chloride, agricultural rare earth, expanded perlite, borax, shell powder, animal bone meal, malic acid, tartaric acid, citric acid, humic acid, fulvic acid, a microbial agent, a microelement fertilizer, a plant growth regulator and a Chinese herbal medicine additive. The fertilizer for improving the stress resistance of corn has the advantages of reasonable formula, high fertilizer utilization efficiency, long fertilizer effect, realization of effective improvement of the soil environment and prevention of soil hardening after being applied, effective improvement of the stress resistance of the corn, promotion of the yield increase of the corn, effective improvement of the quality of the corn, good economic benefit, and promotion of the sustainable development of the agriculture in China.

The invention discloses a drought resistant agent for plants. The drought resistant agent comprises, by weight, 10 to 100 parts of lanthanum chloride, 25 to 100 parts of arginine, 10 to 100 parts of cystine, 25 to 100 parts of aspartic acid, 2 to 50 parts of vitamin B1, 2 to 50 parts of vitamin B2, 1 to 10 parts of selenium element, 2 to 20 parts of plant-growth regulator, 1 to 10 parts of antibacterial and antiseptic agent, 1 to 3 parts of an osmotic agent, 1 to 16 parts of a trace element and 1 to 90 parts of alginate oligosaccharide. A preparation method for the drought resistant agent comprises the following steps: (1) preparing alginate oligosaccharide; (2) preparing a mixture; and (3) carrying out uniform mixing under stirring. The active component alginate oligosaccharide of the drought resistant agent is cheap and can promote prosperity of plant roots, adjust transpiration of plants, enhance the stress resistance of plants and the selenium content of fruit, regulate growth and development of plants, obviously improve in-vitro biological enzyme activity in crops, accelerate metabolism, promote accumulation of permeation substances, and improve water absorption, water retention and free radical removal capability of crops; the drought resistant agent has excellent drought resistance and yield increasing effect; and the preparation method is simple.

The invention discloses a peanut seed coating agent and a preparation method thereof. The peanut seed coating agent takes imidacloprid as an insecticide and amino oligosaccharin as a film forming agent, and is further prepared from two active components including lentinan and harpin protein, and non-active components including a micro-element fertilizer, a wetting dispersant, a thickener, an anti-freezing agent, a de-foaming agent and a safe agent; the peanut seed coating agent is prepared by adopting a wet-process grinding and pulverization process and utilizing a sand grinder and a high-shear emulsifying machine. The peanut seed coating agent disclosed by the invention can be used for effectively controlling insect pests and diseases at a growth period of crops, but also can be used for stimulating seeds to germinate in advance, so that the germination rate of the seeds is improved and the growth of the crops is accelerated; the peanut seed coating agent can also have immunization and killing effects on a plurality of fungi, bacteria and viruses, so that the disease-resisting capability of plants is improved and the production increasing and bumper harvesting of peanuts are guaranteed.

The invention provides a microecological preparation capable of preventing piglets from diarrhoea efficiently. The microecological preparation comprises an enteric soluble wall material and probioticsentrapped in the enteric soluble wall material. An embedding technique can effectively resist strict test in a granulation process, and the microecological preparation can smoothly pass through the stomach to enter intestinal tracts so as to release bacterial strains having vitality. The overall effective content of the probiotics is 50*10<8>CFU / g or above, wherein lactobacillus acidophilus is 30%-40%, bacillus subtilis is 60%-70%, and heat-resisting microzymes are 0.1%-0.2%. Particularly, prebiotics and plant active substances are added to on one hand protect the existing conditions of microecology and on the other hand act on animal organisms in a synergic manner, so that the requirements for comprehensively regulating and controlling growth, development and production of animals can bemet, and the effect is stable and reliable. The key problems for breeding, such as diarrhoea, high medicine resistance, immunosuppression and adverse circumstances, are solved.

The invention provides a continuous gradient bionic manufacturing method based on a pneumatic accurate control active cartilage scaffold. Based on sodium alginate SA ionic crosslinking and gelatin Gel low-temperature solidification forming mechanisms, SA, Gel, hydroxyapatite HA and cartilage cell suspension liquid are used as scaffold base materials, and according to the morphological characteristics of natural soft tissues, an extrusion deposition 3D printing technology based on high-pressure controllable gas is adopted to realize the preparation of an embedded living cell three-dimensional soft scaffold; and in the preparation process, the pressure applied to different charging barrels is dynamically and accurately regulated and controlled in real time, so that the accurate controllability of the component content is realized, then real-time dynamic mixing and continuous gradient laying of different component materials are realized through a dynamic mixing spray head, and the scaffold is crosslinked under the combined action of a CaCl2 solution and a low-temperature platform. The problems that an existing cartilage scaffold material is single in concentration, and the single material components of each layer in a layered structure do not conform to the anatomical characteristics of the continuous gradient of natural cartilage are solved.

The invention discloses a seed coating agent capable of resisting the corn stalk rot diseases and improving the seed vigor. The seed coating agent is prepared from the following ingredients in parts by weight: 3 to 5 parts of bacillus marinus, 0.5 to 1 part of validamycin, 5 to 7 parts of plant bacteriostatic agents, 6 to 10 parts of kieselguhr, 1 to 2 parts of humic acid, 1 to 2 parts of chelatedfertilizers, 0.03 to 0.06 part of dispersing agents, 1 to 2 parts of preservatives and 3 to 5 parts of film formers. The corn seed coating agent can be used for effectively preventing and treating corn stalk rot diseases, effectively supplementing trace elements such as molybdenum, ferrum and boron, regulating the growth and development of corn, improving the soil fertilizer utilization rate andimproving the yield.

The invention discloses a fruit-flavored rice wine. The fruit-flavored rice wine consists of fruits, glutinous rice water, and sweet wine medicine. The production method includes: selecting raw materials, cultivating fruit-flavored distiller's mother, saccharification, pre-fermentation, and post-fermentation. The fruit can be one of kiwi fruit, hawthorn, jujube, lychee, plum, apricot, plum, apple, and pear. The advantages of the present invention are that: the present invention uses fruit and glutinous rice as the main ingredients, and the fruity rice wine is fermented at low temperature to maintain the nutritional components of the fruit, vitamins, carbohydrates, inorganic salts and organic acids in the fruit, especially vitamin C. The nutrients of these fruits are well protected. Fruity rice wine has a pleasant color, aroma, taste and rich nutrition. It has a unique style of orange color, clear, fruity fragrance, sweet and mellow taste, and has the advantages of getting better with age and not bad for a long time.

The invention discloses an organic fertilizer special for tea and a preparing method of the organic fertilizer.The organic fertilizer special for tea is prepared from, by weight, 40-50 parts of chicken manure, 10-15 parts of distiller's yeast, 23-27 parts of rice husks, 5-10 parts of urea, 3-5 parts of calcium superphosphate, 1-3 parts of ammonium sulfate, 0.2-1 part of potassium sulfate, 0.1-2 parts of microelements and 0.1-0.5 part of biological bacteria.The organic fertilizer special for tea has the advantages of regulating the growth and development of plants and improving plant resistance and product quality, soil caking and secondary salinization are not caused when the organic fertilizer is used for a long time, and establishment of a soil granular structure can be prompted; organic nutritive substances and inorganic nutritive fertilizer are combined, and macroelements and microelements are combined; the organic fertilizer special for tea is high in nutrient content, good in water solubility and capable of promoting balanced and strong growth of plants.

The invention discloses a tissue culture quick-breeding seedling raising method for radix asparagi and belongs to the technical field of biology. The method includes the steps of: 1) sterilization; 2) inoculation and callus induction; 3) callus differentiation and adventitious bud induction; 4) seedling reinforcing and rooting cultivation; and 5) domestication and transplanting. A novel rooting culture medium is employed, wherein harmful substances and bacteria in the novel rooting culture medium are eliminated and explant browning is relieved. The rooting culture medium is rich in various beneficial minerals, and has effects of inducing and promoting synthesis of abscisic acid and cytokinin, so that the method can change hormonal equilibrium in the radix asparagi, effectively regulates growth and development of the radix asparagi and promotes and accelerates rooting of the radix asparagi. The method saves time cost and has high rooting rate being more than 95%.

The invention relates to a newly discovered DNA sequence which can be used as a promoter for regulating and controlling specific expression of a target gene in tissue organs of a plant overground part in a photoinduced form. A promoter sequence of an AtcpSecY2 gene is cloned from a model plant arabidopsis thaliana; and subsequently in a transgenic arabidopsis thaliana, the promoter is confirmed to be capable of driving a GUS reporter gene to be specifically expressed in the tissue organs of the plant overground part in the photoinduced form. With application of the promoter, a 'promoter-target gene to be expressed' fusion gene is obtained by construction, a plant is transformed, and the transgenic plant having both plant overground tissue organ specificity and photoinduced specificity expression of the target gene can be obtained. The promoter not only contributes to research on a transcription regulation expression mode of plant genes under a condition of illumination, but also is applied in plant gene engineering to allow exogenous genes to be moderately expressed along with plant physiological statuses under the condition of illumination, at the same time, accurately and effectively changes photosynthetic characteristics and agronomic characters of the plant overground part, realizes crop strain improvement, and has wide application value.

The invention discloses a biological organic and inorganic compound fertilizer which is particles prepared from potassium sulfate, urea, monoammonium phosphate, bentonite, humic acid, a microbial agent and a calcium powder as raw materials; the compound fertilizer contains beneficial active bacteria, can be decomposed into elemental phosphorus and potassium convenient for crops to use, and can accelerate the crop absorption on phosphorus and potassium, and has the functions of self fixing nitrogen, balancing and adjusting various nutrient elements required for the crops, lasting fertilizer efficiency, increasing soil organic matters, preventing soil hardening, and effectively improving a soil ecological structure.

The invention discloses a preparation method for saccharose zinc complex. Saccharose and heptahydrate are subjected to a complex reaction, and a saccharose zinc solution is obtained; then, the saccharose zinc solution is cooled and filtered, filter residues and filtrate are obtained, ethanol water is added into the filtrate, complex precipitates, precipitate is subjected to centrifugal suction filtration and vacuum drying to be dried, and a pure saccharose complex zinc product is obtained; the zinc ion concentration of the filter residues is detected, according to the molar ratio being 1:4 of saccharose and zinc ions, saccharose and heptahydrate continue to be added, waste water obtained after centrifugal suction filtration is returned to a reaction kettle to serve as mother liquid for dissolving saccharose, a circular reaction is conducted, and saccharose zinc continues to be generated. In the process of preparing the saccharose zinc, no resources are wasted, the production cost is reduced, and the production efficiency is improved. The obtained saccharose zinc has the advantages of being safe, efficient and high in cost performance and can be applied to production of animal feed with comprehensive nutrition.

The present invention discloses a mist culture seedling exercising device for millettia specisoa tissue culture seedlings. The mist culture seedling exercising device comprises a mist culture box forexercising seedlings, a temperature and humidity illumination system for controlling temperature and humidity and illumination in the mist culture box, an air pump device for providing spraying powerfor the mist culture box, a liquid storage box for providing a nutrient solution for spraying of the mist culture box and a filter, for filtering the nutrient solution entering the liquid storage box,arranged in the liquid storage box, mutually crossed air flows sprayed by first branch air pipe needle heads and second branch air pipe needle heads of the mist culture box are concentrated on liquiddischarged by branch liquid pipe head ports, and the liquid discharged by the branch liquid pipe head ports are sprayed into an aerosol form so as to realize mist seedling exercising of the millettiaspecisoa tissue culture seedlings. The nutrient solution formed by the mist culture seedling exercising device has fine and evenly dispersed aerosol particles, and the mist culture seedling exercising device is beneficial for roots and stem leaves of the millettia specisoa tissue culture seedlings to fully absorb the nutrient solution, promotes healthy growth, a developed root system and stout stems of the millettia specisoa tissue culture seedlings, and improves a transplanting survival rate and an utilization rate of the nutrient solution.

The invention relates to the technical field of pesticides, in particular to a seed potato coating agent. The seed potato coating agent comprises an active component and a film-forming agent. The active component comprises, by weight, 4 to 8 parts of imidacloprid, 3 to 6 parts of thiabendazole, 2 to 4 parts of uniconazole, 2 to 5 parts of plant growth inhibitor, 8 to 12 parts of dispersant, 0.2 to 0.8 part of trace element fertilizer solution, and 0.2 to 0.6 part of anti-freezing agent. The seed potato coating agent is effective in controlling dry rot and basal stem rot in the potato and is low in cost; the seed potato coating agent is effective in supplementing trace elements such as molybdenum and boron, adjusting growth and development of the potato, improving availability of soil fertilizers and increasing the yield. The seed potato coating agent reaches the standards of like domestic products in terms of stability, film-forming ability and coating strength. By the use of the seed potato coating agent, occurrence rates of the dry rot and basal stem rot in the potato are minimized to 2.8% and 3.2%, respectively.

The invention discloses an interplanting method of anise and mung beans, and relates to the technical field of crop planting. The interplanting method of anise and mung beans comprises the following steps: (1) land selection and land preparation; (2) seed section; (3) seed soaking and pregermination; (4) sowing; (5) field management; (6) mung bean harvesting; (7) fruit set increase of anise trees; (8) anise timely harvesting. According to the interplanting method, the yield of the mung beans can be improved, and the growth of anise trees can further be promoted by using the land maintenance effect of residues after the mung beans are harvested. Moreover, optimized fertilizer components are adopted in each period fundamentally, and nutritional ingredients in fertilizers are sufficiently adsorbed, so that the nutrition required in different periods is sufficient, the growth of the anise trees is greatly promoted, the harvesting time of anise is shortened, and the yield of the anise is improved.

The invention belongs to the field of cell engineering technology, relates to a minisize China rose induction method, and discloses an efficient rapid minisize China rose bud induction method including obtaining of sterile seedlings, China rose bud induction culture, flower culture and other steps. According to the method, pot cultured minisize China rose is used as a plant material, in a stem segment in vitro regeneration system, a most suitable medium for minisize China rose blossoming induction is finally determined as MS + TDZ1.0mg / L + saccharose 40g / L + agar 7.2g / L, test-tube plantlet blossoming is successfully induced, the blossoming rate is increased to 87.1%, a minisize China rose test-tube plantlet little in posture and beautiful and bright in color is successfully cultivated, and the method has important theoretical and practical significance to the China rose asexual breeding.

The invention relates to the technical field of agricultural articles, and specifically relates to a high efficient moisture-retaining drought resistant agent. The moisture-retaining drought resistant agent is composed of the following components in percentage by weight: 1 to 2% of potassium dihydrogen phosphate, 1 to 2% of glucose, 20 to 30% of diatomite, 0.1 to 1% of naphthylacetic acid, 0.1 to 1% of indole butyric acid, 40 to 50% of water absorbing resin, and the balance being water. The invention aims to provide a high efficient moisture-retaining drought resistant agent with multiple functions and ideal application effects.

The invention discloses a water culture seedling hardening method of beautiful millettia root tissue culture seedlings. The method comprises the following steps of 1) making preparation before transplanting: disinfecting a seedling hardening environment and preparing a nutrient solution with the pH value of 5.8-6.5, wherein every 10 L of the nutrient solution contains 0.01-0.05 mg of brassinolide,5-10 mg of nucleotide and other nutritional ingredients; 2) planting the water culture seedlings: planting the disinfected beautiful millettia root seedlings on a planting plate, placing the plantingplate on a cultivation tank filled with the nutrient solution, immersing one part of the root system into the nutrient solution, and not immersing the other part of the root system into the nutrientsolution; and 3) performing water culture management: controlling the temperature, humidity and illumination intensity of the seedling hardening environment according to regulations, replacing the nutrient solution when not more than 1 / 3 of the root system is immersed in the nutrient solution, and hardening the seedlings for a period of time. According to the method, the promotion effect of the brassinolide and the nucleotide is utilized, the moisture and nutrients of the root system are sufficient, and a part of roots are remained to be in contact with air for breathing, so that the root system is facilitated to grow and the transplanting survival rate is increased.