1516 results about "Microbial inoculant" patented technology

The invention relates to a microbial decomposing agent and a production method thereof. The microbial decomposing agent consists of the following components in percentage by weight: 8 to 30 percent of compound microbial inoculant, 40 to 70 percent of organic material and 15 to 35 percent of mineral, wherein microbes in the compound microbial inoculant consist of bacteria, fungi and actinomycetes. The microbial decomposing agent can quickly decompose organic waste.

The invention relates to a group of alive microbial preparations for preparing compound microbial fertilizer and the preparation method thereof. The alive microbial preparations include a microbial inoculant and an organic material degradation agent. The microbial inoculant includes Bacillus megaterium, Bacillus licheniformis, Bacillus mucilaginosus, Bacillus laterosporus, Azotobocter chroococcum, etc. The organic material degradation agent includes Bacillus megaterium, Bacillus licheniformis, Bacillus subtilis, Bacillus circulans, Saccharomyces cerevisiae, Candida lipolytica, Bacillus pumilus, Bacillus cereus, Streptomyces griseus, Pseudomonas alcaligenes, Pseudomonas cepacia, Streptococcus salivarius subsp. Thermophilus, Geobacillus stearothermophilus, Streptomyces Thermophilus, Geotrichum candidum and Aspergillus oryzae. The preparation method comprises the following steps: carrying out submerged fermentation, directly mixing the bacterial liquids and stirring to obtain a liquid preparation; or mixing with an absorbent to obtain a solid preparation. The alive microbial preparations have the advantages of improving soil fertility, promoting plant growth, enhancing anti-disease, anti-insect and anti-drought capacities of plants, improving quality of agricultural products and so on.

The invention relates to a bio-organic compound fertilizer, which comprises the following components in part by weight: 10 to 30 parts of organic matter, 2 to 10 parts of compound microbial inoculants, 5 to 20 parts of humic acid, 5 to 20 parts of amino acid, 4 to 10 parts of chitin, 1 to 5 parts of polypeptide, 530 parts of nitrogen, phosphorus and potassium, 5 to 10 parts of secondary and trace elements, 5 to 15 parts of algae fertilizer, 5 to 10 parts of zeolite powder and 1 to 5 parts of synergistic agent. Through the scientific and rational formula, the bio-organic compound fertilizer achieves nutritive equilibrium and can satisfy the need of crop growth in nutrient. The microbial inoculants are added to quickly degrade natural macromolecular organic compounds into the organic fertilizer and decompose organic pollutants, which effectively improves the capacity of crops in the absorption of nutrient components, reduces the waste of the fertilizer and reduces the damage of the chemical fertilizer to soil to further achieve the nutritive equilibrium, satisfy the need of the crop growth in the nutrient, achieve the aims of low investment and high output value, improve the quality of the crops and improve the economic benefit brought by the crops.

The invention relates to a pest-proof disease-resistant organic fertilizer and a preparation method thereof, belonging to the technical field of organic fertilizers. Tobacco waste and oil tea pulp are utilized as main raw materials to develop a method of the pest-proof disease-resistant organic fertilizer. The invention breaks through the limitations of harmful substances and single product effect due to addition of chemical materials in the traditional organic fertilizer. The pest-proof disease-resistant organic fertilizer is prepared from the following raw materials: tobacco waste, oil tea pulp, organic substances, a poising agent and a microbial inoculant. The preparation method of the organic fertilizer comprises the following steps: crushing tobacco straws, tobacco stems, tobacco powder, oil tea pulp and organic substances, spraying the poising agent and microbial inoculant, mixing, carrying out pool stack fermentation and strip stack fermentation, screening and granulating to obtain the insect-proof disease-resistant organic fertilizer. The high-efficiency biological insect-inhibiting factors contained in the organic substances containing massive mycoprotein can effectively inhibit and kill pests and pathogenic bacteria, and solve the problem of environmental pollution caused by the pesticide fertilizer.

The invention discloses a refined organic fertilizer and preparing method of sludge, which comprises the following steps: compounding piled raw materials; piling; turning the pile; ripening; drying in the hot cavity; sieving to obtain the product. The refined organic fertilizer contains composite sludge, organic amendment, inorganic amendment, matured accelerant, microbe grafting agent and adsorbant, which are blended completely according to certain proportion, wherein these materials are fermented for the second time, which are prepared into refined organic fertilizer.

The invention discloses a method for manufacturing a fermentation bed for livestock cultivation and a method for breeding pigs on the fermentation bed. The livestock cultivation fermentation bed is characterized by being manufactured by paving a loess layer on a foundation layer of an organic gasket and sprinkling sodium chloride, brown sugar and strains consisting of compound microbial inoculants where effective microorganisms and indigenous microorganisms coexist, rice bran and wheat bran on the loess layer. Green feed serves as main feed for the pigs on the fermentation bed, and energy feed and supplementary feed consisting of Chinese medicaments having the functions of benefiting gastrointestinal health, facilitating digestion and expelling toxin to improve the immunity of the pigs also can be fed to the pigs. Compared with the prior art, the livestock cultivation fermentation bed has the advantages that the pigs bred on the bed have strong immunity, need no vaccines and antibiotic and can be fed with the green feed for bulls and sheep; and the meat of the bred pigs has the same pleasant taste as wild pigs.

The invention discloses a preparation method of a functional organic fertilizer for soil restoration. The method comprises the following steps: mixing crushed straws with blue-green algae according to the mass ratio of (1-5):10 to obtain a mixture, and then adding a biomass-transformed complex microbial inoculant and a biomass-transformed inductive material to the mixture; evenly mixing and then carrying out conventional stacking fermentation for 5-15 days, so as to obtain a semi-solid organic fertilizer; and mixing and pelletizing the semi-solid organic fertilizer, clay, biomass carbon ash and a heavy metal ion chelating agent according to the ratio of 1000:(50-200):(50-150):(1-5), so as to obtain the functional organic fertilizer for soil restoration. The organic fertilizer disclosed by the invention has a persistent slow-release fertilizer effect, and can adsorb, chelate and immobilize the heavy metal ions in a deactivation manner, soil fleeciness is facilitated, the moisture retention and the breathability are improved, rooting of plants is facilitated, formation of beneficial components of soil microelements and the like is optimized, the heavy metal-polluted soil is effectively restored, and secondary pollution of the soil is not caused.

The invention relates to a biological denitrification method for salt-containing sewage, which comprises the following steps: adding a denitrification microbial inoculant into a biochemical sewage treatment system, and simultaneously starting the nitrification-denitrification biological denitrification treatment processes, wherein the sewage treatment temperature is 18-40 DEG C, the dissolved oxygen is 0.1-5 mg/L, and the pH value is 7.0-9.0. The denitrification microbial inoculant contains one or two of Staphylococcuscohnii FSDN-C, Arthrobactercreatinolyticus FDN-1 and Flavobacteriummizutaii FDN-2, and also contains one or two of Paracoccusdenitrificans DN-3 and Methylobacteriumphyllosphaerae SDN-3. The method provided by the invention can effectively treat high-salt-content sewage, enhances the adsorptivity and flocculence for sludge, has wide application range for wastewater quality and high impact resistance to salt-containing sewage, and obviously enhances the sewage treatment effect on the premise of removing pollutants, such as ammonia nitrogen, COD (chemical oxygen demand) and the like.

The invention provides a novel microbial fertilizer special for tobaccos. The fertilizer comprises the following components in parts by weight: 5 to 25 parts of a complex microbial inoculant and 75 to 95 parts of a carrier, wherein the complex microbial inoculant contains 1.6 to 8 hundred million cfu/g of bacillus subtilis, 1.6 to 8 hundred million cfu/g of bacillus licheniformis, 0.4 to 2.0 hundred million cfu/g of trichoderma harzianum and 0.4 to 2.0 hundred million cfu/g of calicheamicin streptomyces hygroscopicus; the carrier is a powdered material which are made by performing microbe fermentation and decomposition on rape seed cakes, bean pulp cakes and sesame-seed cakes, and then performing drying and sieveing. The microbial fertilizer contains bacterial strains for antagonizing fungus pathogenic bacteria, bacterial strains for preventing and curing the breeding of soil pathogenic bacteria, bacterial strains for improving the soil environment and the like. The carrier is fermented three-cake fertilizer. The invention also provides a preparing method for the microbial fertilizer. The microbial fertilizer special for the tobaccos, provided by the invention, is powder, is capable of absorbing the water and preserving the moisture. The fertilizer is mainly used as the base fertilizer when fertilizing, the soil fertility can be improved, the nutrient absorption of crops is improved, the diseases of a plurality of crops can be prevented, and the quality and the yield of tobacco leaves can be effectively improved.

The invention discloses complex microbial inoculant which comprises the following active ingredients: bacillus subtilis, bacillus megatherium, trichoderma koningii, aspergillus oryzae, trichoderma viride, geotrichum candidum, aspergillus niger, rhizopus oligosporus, lactobacillus plantarum CICC20768 and azotobacter chroococcum. Correspondingly, the invention further discloses a method for preparing an organic fertilizer from the complex microbial inoculant. The method comprises the following steps: a. uniformly mixing the complex microbial inoculant with a plant fiber raw material to obtain a fermented raw material; b, composting and fermenting the fermentation raw material; c, drying the composted fermentation raw material to obtain the organic fertilizer. microbes of the complex microbial inoculant have no antagonism, and can effectively cooperate in the production process of the organic fertilizer, and the chemical components of the production raw materials can be sufficiently utilized to finally produce the high-quality organic fertilizer. The produced organic fertilizer reaches the parameter indexes of the standard (NY884-2012) of China, can improve the planting property of soil, is beneficial to optimization of soil microbial flora, and can provide comprehensive nutrients to a plant root system.

The invention discloses a special fertilizer for fruit trees and a preparation method thereof. The fertilizer comprises a base fertilizer and a leaf fertilizer, wherein the base fertilizer is prepared from the following raw materials: an organic fertilizer, urea, monoammonium phosphate and potassium chloride, the organic fertilizer is prepared by fermenting raw materials, namely dry chicken manure, cornstalk, rice husk, fruit tree leaves, bean cakes and needle mushroom dregs by virtue of complex microbial inoculants, and the leaf fertilizer comprises the following components: urea, monopotassium phosphate, farm-oriented chitosan oligosaccharide, 6-BA, multi-effect triazole, triazolone, polyethylene glycol 200, sodium tetraborate and microelements. The fermented organic fertilizer not only contains a large quantity of beneficial bacterium, organic matters and multiple microelements, but also can be used for increasing the beneficial bacterium of the soil, fix nitrogen, dissolve phosphorus, activating the soil after being applied, improving the water retentivity, fertilizer using efficiency and fertilizer persistent effect of the soil, and achieving the effect of a controlled-release fertilizer; in addition, the leaf fertilizer is applied in different growing stages of the fruit trees, thus supplementing nitrogen, phosphorus, potassium and microelements, promoting to bear and expand fruits, and improving the stress resistance of the fruit trees.

The invention relates to an immobilized microbial inoculant for restoring petroleum-hydrocarbon-polluted alkaline-saline soil and a preparation method thereof. A salt/alkali-tolerant petroleum hydrocarbon degradation bacterium variant corynebacterium B05: (Corynebacterium variabile) HRJ4 (the collection number is CGMCC No.10134) is screened from alkaline-saline petroleum-polluted soil, and sodium alginate is added into a carrier biochar to prepare immobilized bacterium pellets which are stored and used for treating petroleum-hydrocarbon-polluted soil. The immobilized bacterium pellets are prepared by mixing the bacterium suspension, biochar and sodium alginate in a v/w/w ratio of 100:5:2. The invention provides a preparation method of the immobilized bacterium pellets. The immobilized microbial inoculant has the advantages of simple technique, obvious advantages of the degradation bacterium, and high stability, is beneficial to the petroleum hydrocarbon degradation bacterium to adapt to the new polluted environment, enhances and improves the microecological environment quality of the soil, does not cause secondary pollution to the environment, and can obviously enhance the degradation efficiency of petroleum hydrocarbons in the alkaline-saline soil.

A method of plant cultivation which comprises inoculating plants in a plant growing system comprising a container and an insert therefore, the microbial inoculant containing at least one species from the following group of microorganisms comprising arbuscular mycorrhizae associated bacteria, plant growth promoting rhizobacteria, yeast microorganisms and substrate conditioning bacteria.

The invention relates to a method for preparing an algal biological feed. The method comprises the following steps of: preparing complex microbial inoculants from actinomycetes, saccharomycetes, bacillus subtilis, lactobacillus casei and lactobacillus; and fertilizing algas, and under the synergistic effect of a plurality of strains, generating a mass of cellulase type degraded fibers while synthetising mycoprotein by taking full advantage of an inorganic nitrogen source, an organic nitrogen source and saccharides generated through degradation of the fibers. The algal fermented biological feed provided by the invention has more essential amino-acids and higher nutritive value as compared with algas; animals can absorb the feed quickly and the daily gain can be increased by 4-7%; besides, the immunity of the animals is enhanced, the serum alkaline phosphatase activity is improved by 15% and the alanine transaminase activity is increased by 6%; and the breeding effect is quite obvious. In addition, the preparation method provided by the invention has the advantages of low production cost and short period in contrast with the prior art.

The invention provides a method for producing an organic fertilizer from municipal dry branch/fallen leaf waste and application thereof. The method comprises the following steps: collecting branches, fallen leaves and other municipal greening wastes, and mixing Aspergillus niger, Saccharomyces cerevisiae, Bacillus subtilis and Trichoderma reesei to obtain a composite microbial inoculant; adding bran coat, chaff powder or peanut hull meal, the composite microbial inoculant, urea and potassium dihydrogen phosphate, thoroughly mixing, piling into a 1.5-2m-high cone, and controlling the water content at 60-70%; carrying out hide lifting on the pile in the composting process, and fermenting for 20-30 days; and carrying out aerobic and anaerobic fermentation on the dry branch/fallen leaf waste by using the microbial inoculant to obtain the organic fertilizer. The preparation technique is simple; and the prepared organic fertilizer can improve the soil and reduce the environmental pollution caused by the waste.

The invention discloses a method for purifying mariculture wastewater by utilizing a compound microbial inoculant. The method comprises the following steps: (1) screening the compound microbial inoculant which consists of photosynthetic bacteria, nitrobacteria, denitrifying bacteria, candida utilis, lactobacillus acidophilus and bacillus; (2) activating the compound microbial inoculant by utilizing a culture medium: directly adding the strain into the culture medium based on 1% of inoculation amount (w/v), and activating the strain by a shaker at the temperature of 37 DEG C with the speed of 150rpm (revolution per minute) for 24 hours so that the total content of microbes is 7*10<8>-9*10<8>CFUs (colony forming units)/mL; and (3) adding the activated compound inoculant into the treated aquaculture wastewater, wherein the compound inoculant accounts for 0.06%-0.12% of the total weight of the aquaculture wastewater. The method has the advantages of no toxicity and harm on aquaculture animals, simple process, fast organic matter degradation speed, obvious effect, no drug residues and no secondary pollution. Therefore, the method has economic feasibility and is applicable to popularization and application to coastal aquaculture industry.

A dry, solid composition comprising humate combined with a phosphate source is disclosed which significantly increases the phosphate absorption efficiency of phosphate fertilizers. Solid humate/phosphate fertilizer compositions, optionally comprising a binding agent containing a soil microbe food source and/or a phosphate-solubilizing microorganism inoculant, their use, and processes for manufacturing these compositions are disclosed.

The invention provides a composite microbial inoculant for controlling plant continuous-cropping diseases, which comprises trichoderma harzianum, bacillus subtilis, and gliocladium roseum, and also comprises phosphate-solubilizing bacteria and potassium-solubilizing bacteria; the accession number of the trichoderma harzianum strain is CGMCC NO. 7861; the accession number of the bacillus subtilis strain is CGMCC NO. 7850; the accession number of the gliocladium roseum strain is CGMCC NO. 9008. The invention also provides a preparation method and an application of the composite microbial inoculant. Through the selection of high-efficient bacterial strains such as disease-preventing trichoderma, bacillus subtilis, gliocladium roseum, phosphate-solubilizing bacteria and potassium-solubilizing bacteria, the composite microbial inoculant of the invention promotes the growth of crops, improves the utilization rate of fertilizers, enhances the stress resistance such as disease resistance and the like, improves the quality of crops, and can control diseases such as root rot, verticillium wilt, damping off, botrytis, epidemic diseases, powdery mildew, sclerotinia rot, seedling-stage damping off, and the like.

The invention relates to an antagonistic bacterium for controlling leaf spot of peanut and a microbial preparation thereof, belonging to the field of biological control on crop disease. The invention provides a Bacillus amyloliquefaciens BA-KA1 of which the collection number is CGMCC No.8341 and a microbial inoculant containing the same, and also provides application of the Bacillus amyloliquefaciens or the microbial inoculant in control on leaf spot of peanut. According to the invention, the Bacillus amyloliquefaciens BA-KA1 of which the collection number is CGMCC No.8341 and the microbial inoculant thereof have obvious antagonistic action on leaf spot bacteria of peanut, and can control occurrence of leaf spot of peanut; the control efficiency is above 70%. The use of the strain and the microbial preparation thereof can reduce application of chemical pesticides in peanut production processes, thus having favorable ecological and social benefits.

The invention relates to a preparation method of aerobic high-temperature static compost and discloses a preparation method of the aerobic high-temperature static compost by using corrosion-promoting compost composite fermentation bacteria inoculants. The preparation method includes that an indigenous microorganism strain indigenous bacteria agent and thermophilic bacteria thermophilic fat bacilli are combined to be prepared into composite microbial inoculants, temperature of a pile rises rapidly more than 55 DEG C in early access of a compost substrate, previous time of pile fermentation is shortened, activity of substrate microorganism is enhanced, and the compost enters a high-temperature stage in a shortest time. Through activity of thermophilic bacteria and oxygen leading in the pile, rapid decomposition is achieved, and therefore compost quality is improved.

The invention relates to a composite microbial inoculant and its preparation method, and overcomes the problems of slow decomposing agent start and incomplete decomposition in the prior art. The invention provides a straw decomposing agent for quick start of decomposition at low temperature, and the straw decomposing agent comprises a strain YO-H, bacillus subtilis, Saccharomyces cerevisiae, Aspergillus niger and Trichoderma harzianum. The straw decomposing agent provided by the invention has the advantages that: 1. it employs the strain YO-H, and can quickly start decomposition under the condition of a low environmental temperature; 2. the strain YO-H cooperates with other decomposing fungi to achieve a better decomposing effect; and 3. an additional enzyme preparation is added to improve the cellulose activity of the decomposing agent, about 3-4 days are left before mass propagation of decomposing fungi and generation of new enzyme, and during the period, the additionally added enzyme preparation is employed to degrade lignin and cellulose, that is to say the decomposition time is 3-4 days ahead of schedule.

The invention relates to a Bacillus subtilis strain YB-05, and a preparation method of an inoculant of the Bacillus subtilis strain YB-05. The Bacillus subtilis strain YB-05 is preserved at China General Microbiological Culture Collection Center, and has a preservation number of CGMCCNO.6498. The microbial inoculant is a pulvis prepared through the spray dry preparation of a fermentation solution of the Bacillus subtilis strain YB-05. The YB-05 strain and its inoculant have the advantages of wide antibacterial spectrum, good antimicrobial effect, and strong inhibition effects on the wheat take-all disease, tomato Botrytis cinerea, wheat sheath blight fungi, Phytophthora melonis and the like; and the inoculant has the advantages of high efficiency, non-toxicity, simple use, and no environmental pollution.