66 results about "Hypha" patented technology

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The application discloses a bucket type cultivation method for overcoming continuous cropping obstacles of bamboo shoots. Through the bucket type inoculation, the mycelium is effectively isolated from the miscellaneous bacteria and pathogenic microorganisms in the soil before the mycelium completes the dominant expansion, the mycelium expansion stability is obviously improved, and the mycelium death and yield loss caused by the continuous cropping obstacles are avoided. Field tests show that the yield of the second year can be recovered to more than 90% of the yield of the first year by using the method, and the yield of the second year is less than 10% of the yield of the traditional reseeding mode, and almost no yield is obtained, so the method has good stability and generalizability.

This application belongs to the fields of microbiology and cell biology, and provides a method for directly preparing germination-promoting fungal protoplasts from orchid protocorms, comprising: (1) physical homogenization; (2) filtration; (3) removal of plant contents and residual cell walls; (4) purification of mycelial clusters; and (5) release and purification of fungal protoplasts. Through the rational design of homogenization and purification steps, this application achieves high-yield, high-purity, and high-vitality preparation of germination-promoting fungal protoplasts, providing excellent materials for orchid gene variety breeding and genetic engineering operations.

A cultivation method for improving the quality and yield of black fungus using hydrogen-rich water involves spraying a hydrogen-rich water composition three times during mycelial culture, spraying it every two days during primordium differentiation, and spraying it daily during fruiting body enlargement. The hydrogen-rich water composition comprises hydrogen-rich water, xylose, humic acid, gelatin, hydroxypropyl cellulose, and deionized water. This invention effectively solves the problems of poor hydrogen concentration stability and hydrogen volatilization in hydrogen-rich water by adding functional components such as gelatin, hydroxypropyl cellulose, xylose, and humic acid to the hydrogen-rich water, thus extending the time of hydrogen concentration stability and its effect. By using the above-mentioned hydrogen-rich water composition during the mycelial growth and primordium differentiation stages of black fungus, the microenvironment for black fungus growth is comprehensively optimized, resulting in increased yield, improved quality, and enhanced marketability. This provides an effective way to cultivate superior black fungus varieties.

The application discloses a kind of low temperature resistant strain YW-1 for preventing and treating rice seedling wilt, and belongs to the field of plant protection.The low temperature resistant strain YW-1 is a pseudo-odor class fungus, and the application proves that the strain YW-1 can maintain normal growth and metabolism in a low temperature environment of 15 DEG C, and can effectively destroy the hyphal structure of the wilt pathogen by secreting cellulase and chitinase, thereby inhibiting the occurrence of the disease from the source.The strain YW-1 has no antagonism with the commonly used functional fungus Trichoderma in the seedling raising substrate, and the two can be compounded and coexist, thereby realizing the synergistic effect of functional microorganisms.The application overcomes the defects of the existing biocontrol strain, such as reduced activity in a low temperature environment and unstable prevention effect, and provides a stable and reliable biological control scheme for factory seedling raising in a low temperature season without increasing the amount of chemical pesticides, thereby significantly improving the quality of seedlings and the seedling raising success rate.

The utility model provides a kind of edible mushroom multilayer three-dimensional cultivation frame, it is specifically related to edible mushroom technical field, it is characterized by: two groups of chain rotation tracks are symmetrically arranged in the inside of cultivation support frame two sides, and the surface of two groups of chain rotation tracks is fixedly connected with multiple fixed racks, each fixed rack is connected with fixed shaft, and the other end of each fixed shaft is fixedly connected with support frame top, support platform is horizontally arranged in support frame, and rotating shaft is connected in the both ends of support platform, and the both ends of rotating shaft are connected with support frame, and every two symmetrical support frames are connected with a support platform, and every support platform is parallel, and cultivation box is arranged on the surface of support platform;The utility model is through the synergistic effect of chain rotation track and gear transmission, and support platform can be circularly displaced along rotation direction, and different growth stages of mycelium can be observed in time, effectively solve the problem that ventilation is blocked in later stage of fungus stick, and uniform illumination radiation covers each layer of mycelium, and the quality of mycelium development is significantly improved.

The present application relates to the technical field of genetic engineering, and particularly relates to a fruiting inhibition engineering bacterium and a construction method and application thereof, comprising overexpressing a transcription factor Pcc1 in a ganoderma strain, constructing an engineering bacterium MKLGE253241 with high expression of Pcc1, a preservation unit: China General Microbiological Culture Collection Center, address: No. 1, Xibei Road, No. 3, Chaoyang District, Beijing, preservation date: March 11, 2026, preservation number: CGMCC No. 42592. The present application blocks the formation of fruiting body primordium from the molecular level, maintains the mycelium in vegetative growth, and increases the average biomass by 15.2%, without fruiting body; meanwhile, the leather flatness of the mycelium produced by the engineering bacterium is significantly improved, and the scale qualified rate is obviously improved. The present application fundamentally solves the problem of fruiting body leather, avoids chemical inhibitors, and provides a new path for high-performance bio-based leather.

This invention relates to the field of fungal strain cultivation technology, and particularly to a method for isolating and cultivating fungal strains from matsutake fruiting bodies. The method involves collecting matsutake mushrooms that are 70%–80% mature and free from insects, disinfecting them with alcohol, longitudinally splitting them in half, removing the flesh from the cap and stipe, and inoculating them onto a slant culture medium to obtain a primary strain. This primary strain is then inoculated into a culture medium and shaken to obtain a primary shake flask strain, which is then inoculated into a culture medium. The strain is then shaken and cultured, and the concentration of metabolites, dissolved oxygen, and pH in the culture medium are collected in real time to construct a strain growth time prediction model to predict the stage culture duration. The cell proliferation trend is determined based on the number of matsutake mycelial cells. Based on a comparison of the actual culture time during the initial adjustment period and the stage culture time, the adjustment method for the logarithmic growth phase of the current batch is determined. Based on a comparison of the shake flask culture time and the predicted culture time, the adjustment method for the initial culture conditions of the next batch is determined. This invention effectively optimizes shake flask culture conditions and improves strain quality.

The utility model relates to stirring device technical field, especially edible fermentation with stirring device of edible fungus, including inside hollow jar body, the jar body outside is provided with feed inlet and discharge gate, be provided with motor on the jar body, the pivot that is in the motor output end and extends to the inside of jar body and the paddle that is in the pivot periphery, the paddle is made of swellable material, through the swelling morphological change of control paddle can match the shear force required in different stages of edible fungus fermentation in real time, both protect the mycelium and guarantee the stirring effect, the control valve is arranged on the pivot and exposes in the side of jar body outside, the inside hollow area of pivot and swellable chamber in the paddle inside communicate, can control fluid in and out through the opening and closing state of control valve and the fluid supply device outside, realize the control of paddle swelling morphological, more convenient and faster reach the shear force required in the current stage.

The application relates to the technical field of edible fungus inoculation, and in particular to a chondrostereum bag inoculation device, which comprises a supporting frame and an inoculation device, the inoculation device comprises an inoculation box fixedly connected to the top of the supporting frame; two sealing plates are arranged in the scheme; after a fungus bag enters the inoculation box, the sealing plates are lowered to the bottom to form a completely closed space, the inoculation process is isolated from the open environment, bacteria spores (such as penicillium and aspergillus) in the air can be avoided from falling into the opening of the fungus bag or the inoculation position, the inoculation needle is made of soft silica gel material, can be slightly deformed with pressure when being inserted into the fungus bag, rigid extrusion and scratch of chondrostereum mycelium caused by traditional metal needles are avoided, the electromagnetic valve and the spiral blade are matched with the elastic sealing valve to send the seed cylinder, quantitative conveying is realized, growth differences caused by too much inoculation (intense nutrition competition) or too little inoculation (mycelium is difficult to quickly occupy space) can be avoided, strain waste caused by excessive inoculation is avoided, and the raw material cost can be significantly reduced in the long term.

The application discloses a kind of bat moth larvae double bacteria synergistic infection methods, belong to microbial engineering and medicinal fungus cultivation technical field, comprising: 1-2 age larvae are preprocessed by containing first probiotic feed feeding, optimize intestinal microenvironment;3-4 age larvae are injected and infected using mixed bacterial liquid containing second probiotic and Chinese hairy hair fungus, promote spore germination and colonization;5 age and above larvae are stably cultivated by third probiotic spraying, whole process is matched with dynamic monitoring and threshold emergency control.The technology adopts age differentiation operation of low-age feeding-middle-age accurate injection-high-age spraying, matches each stage functional targeted probiotic, constructs suitable mycelium germination microenvironment by means of probiotic metabolite, inhibits miscellaneous bacteria pollution and provides adenosine synthesis precursor, realizes the excellent effect of infection rate improvement, mortality reduction, adenosine content improvement and effective content improvement, provides efficient feasible technical path for large-scale high-quality cultivation of cordyceps sinensis.

The application discloses a preparation method of mycelial pellet composite material for enhanced denitrification of micro-polluted water, and comprises the following steps: screening and obtaining a bacterial strain and a fungal strain, and preparing a bacterial suspension; culturing the fungal strain to prepare mycelial pellets; culturing the bacterial suspension in a mycelial pellet culture medium to prepare DMP; modifying biochar by KMnO4 to prepare modified biochar, and preparing a modified biochar suspension; adding the modified biochar suspension and a chlorella suspension into the DMP, and oscillating and culturing to prepare the mycelial pellet composite material. The mycelial pellet composite material for enhanced denitrification of micro-polluted water prepared by the application has the characteristics of structural stability, high electron transfer efficiency and strong adaptability.

The application provides a fluorescent image recognition system for detecting fungi based on a deep learning algorithm, comprising an image acquisition module, a single spore and mycelium recognition module and a mycelium cluster recognition module; the image acquisition module is used for obtaining a target fluorescent image; the single spore and mycelium recognition module and the mycelium cluster recognition module are used for inputting the target fluorescent image into a pre-established deep learning algorithm model, recognizing single spores or mycelia and mycelium clusters through the pre-established deep learning algorithm model, and outputting fungal negative or positive results according to the recognition results. Compared with the prior art, after the image acquisition module obtains the target fluorescent image, the target fluorescent image is input into the pre-established deep learning algorithm model for automatic analysis, and the analysis of spores / mycelia and mycelium clusters that are different in morphology is divided into two modules for processing, so that the spores, mycelia and mycelium clusters can be more accurately recognized and distinguished.

The present application relates to the field of intelligent agricultural mycorrhizal fungus fertilizer production and application, and discloses a method and application for intelligent soil testing, mycorrhizal fungus fertilizer preparation, quality improvement and soil improvement, which is based on the use of full spectrum direct reading plasma emission spectrometry IPC-OES / ICP-MS, elemental analyzer VarioEL, ion chromatography HPIC combination, and qualitative and quantitative exchange capacity CEC, discloses a method and application for intelligent soil testing, mycorrhizal fungus (AMF) fertilizer preparation, quality improvement and soil improvement by using a chlorophyll nitrogen dynamic monitoring (SPAD value) instrument, a phosphorus online detector and a potassium online detector, which uses a double cultivation and propagation method of mycorrhizal fungus hypha secretion of sphaerocystin, activates plant related gene pathways, improves the quality of crops such as food, vegetables, tobacco leaves and Chinese herbal medicines, and the drought resistance, cold resistance and disease resistance of the crops, forms soil aggregates, increases soil porosity, effectively relieves compaction, and reduces the use amount of chemical fertilizer by 60-80% and pesticide by 70-90%.

The application belongs to the technical field of microorganisms, and discloses a propionic acid-producing propionic acid bacteria NHNK-621 and application of the propionic acid bacteria in preparation of products for improving constipation and memory, the bacteria strain has been preserved in the China Center for Type Culture Collection on November 25, 2024, and the number is CCTCC NO: M 20242631. The propionic acid bacteria NHNK-621 provided by the application has the functions of promoting 5-HT secretion, up-regulating intestinal aquaporin and brain-derived neurotrophic factor genes, metabolically producing gamma-aminobutyric acid, inhibiting growth of Candida albicans, inhibiting hypha formation of Candida albicans, combining with hypha of Candida albicans, up-regulating intestinal defensin and mucin gene expression, in addition, the bacterial biofilm can increase the colonization ability to intestinal epithelial cells, and can be used for preparing products for improving constipation and memory.

This invention relates to the field of plant disease biological control technology, and particularly to a Fusarium oxysporum cell wall extract, its preparation method, and its application. The extract is derived from the polysaccharide fraction of the cell wall of Fusarium oxysporum spores or hyphae, and contains at least one pathogen-associated molecular pattern (PAMP) component. This component is a non-protein, thermally stable, species-nonspecific elicitor capable of forming an aqueous dispersion system. The extract of this invention can be recognized by plant PRRs and activate PTI immunity. Rehmannia glutinosa tubers treated with the extract showed a significantly increased germination rate after inoculation with Fusarium oxysporum. It also induces superoxide anion production, upregulates the activities of superoxide dismutase, peroxidase, and catalase, and enriches differentially expressed genes in the MAPK signaling pathway, jasmonic acid metabolism pathway, and phenylpropane biosynthesis pathway. Furthermore, the extract can directly inhibit Fusarium oxysporum colony expansion, conidia production, and mycelial growth. Extracts derived from spores or hyphae show no significant difference in inducing disease resistance, and the raw materials are flexible and easy to industrialize. This extract is derived from the cell wall of the pathogen itself, making it environmentally friendly and free from the risk of chemical pesticide residues. It is suitable for the green control of root rot disease in Rehmannia glutinosa.

The application discloses a bat moth lepidoptera cordyceps strain and application thereof in preparation of anti-neutron radiation medicines. Samsoniella hepiali The strain is the bat moth lepidoptera cordyceps strain P969 (Pleurotus spodoleucus), which is preserved in the China Center for Type Culture Collection and has a preservation number of CCTCC NO: M 20251172 and a preservation date of May 26, 2025. Mycelium extracts of the strain can protect biological tissues against neutron radiation and repair organ damage caused by neutron radiation, so that the strain has a wide development space and a good development and application prospect.

The present application relates to mycelium culture technical field, especially edible mushroom automatic detection culture device, including incubator, the top inner wall of incubator is provided with infrared plant growth lamp, and its outer wall is connected with the sliding door, the inner wall of sliding door is provided with culture base, the humidity adjusting mechanism is set, when the culture base inside culture solution content reduces, the piston can enter the cylinder in the bottom of first water tank, then through first spray head, the culture solution is supplemented to the mycelium root, and through the extension of first electric push rod, the spray amount of first spray head can be adjusted in a certain range, the humidity of mycelium root is controlled, when the mycelium growth environment is too dry, the clean water in second water tank is sprayed to the incubator through second spray head, so that the mycelium growth environment is humidified, and the environmental humidity and root culture solution content are controlled according to the actual growth needs of mycelium.

The present invention relates broadly to methods of producing Mycelium-based materials, to Mycelium-based products, and industrial applications thereof. Non-limiting example embodiments of the present invention aim to enhance the production process of a mycelium-based composite by employing a combination of specific mechanical, chemical and manufacturing processing techniques to improve the bonding between the mycelium hyphae and the substrates and / or to enhance the microstructure of the final composite / product and / or to enhance production efficiency of final composite / product.

The application discloses use of an extract of black tiger in preparation of a fungicide for preventing and treating plant diseases caused by pathogenic fungi and oomycetes. The black tiger extract has the effects of inhibiting spore germination, germ tube growth or zoospore formation, and vegetative hypha growth of the fungi and oomycetes, can effectively prevent growth of the pathogenic fungi and infection on host plants, and has certain prevention and treatment effects on rice rice blast, rice rice smut, potato late blight, cucumber anthracnose, grape anthracnose, corn ear rot, pepper blight and camphor blight, and provides a possibility for development of a plant source fungicide with diversified effective components, high prevention and treatment efficiency and a wide spectrum of prevention and treatment objects.

The fungi remove dirt by using their ability to hold and contract the substrate with their hyphae, allowing them to lie dormant. [Solution] Spray water containing dissolved mycelium to make it fall off in flakes.

The application discloses application of serine protease AprE and a coding gene thereof in prevention and treatment of rice blast, and belongs to the technical field of biotechnology.The amino acid sequence of the serine protease AprE is shown as SEQ ID NO.13, and the nucleotide sequence of the coding gene is shown as SEQ ID NO.14.It is found by the application that after the serine protease encoded by the aprE gene is used to treat blast fungus hypha cells, abnormal ROS accumulation occurs in the cells, and the hypha cells are caused to be apoptotic, broken and abnormally expanded, so that the pathogenic bacteria are finally killed.The aprE gene can be used to study biochemical nature of inhibition of the blast fungus, and can also be used as a target gene of a high-efficiency biocontrol strain and applied to biological prevention and treatment of rice blast.The recombinant serine protease can also be directly used for prevention and treatment of rice blast and reduction of pathogenicity of pathogenic bacteria.

This invention provides a method for mutagenesis of *Aspergillus cristatus* and its application, belonging to the field of microbial technology. The mutagenesis method provided by this invention includes the following steps: first, pretreating *Aspergillus cristatus* suspension by mixing it with a compound enzyme solution; then culturing the pretreated *Aspergillus cristatus* in a culture medium supplemented with a mutagen; the compound enzyme solution consists of a cell-lysing enzyme solution and a β-glucanase solution; the mutagen includes ethyl methanesulfonate and / or diethyl sulfate. This invention can improve the efficiency of the mutagen entering the bacterial cells, thereby enhancing the mutagenesis effect; the mutant strains obtained by mutagenesis, compared to the original strain, exhibit more vigorous colony growth on plates, larger average diameter, denser hyphae, and / or better morphological stability. Furthermore, it can achieve ideal mutagenic selection pressure at lower mutagen concentrations and shorter treatment times, reducing sublethal damage, which is beneficial for the recovery and screening of mutant strains and improving the efficiency of mutagenesis experiments.

The application discloses a selenium-rich Phellinus baumii strain domestication, cultivation and selenium enrichment method, which follows the gradient domestication principle of low-selenium starting and gradual increase, sequentially completes selenium source domestication of the Phellinus baumii primary mother strain, secondary original strain and tertiary cultivation strain, and obtains the cultivation strain suitable for the high-selenium environment; then, the tertiary cultivation strain is taken as the inoculation source, standardized cultivation is carried out by adopting the bagged solid culture medium, and the temperature, humidity, light and air conditions are regulated and controlled in two stages of mycelium culture and fruiting body growth management; and through gradient selenium source supply, continuous selenium supplement during the cultivation period, accurate regulation and control of the environmental conditions and scientific treatment after harvesting, efficient absorption and conversion of selenium by the Phellinus baumii are realized, inorganic selenium is converted into organic selenium, and the organic selenium is enriched in the fruiting body. The application solves the problems of low selenium tolerance and poor selenium enrichment efficiency of the Phellinus baumii, the selenium-rich Phellinus baumii fruiting body has high and stable organic selenium content, and the strain domestication success rate and cultivation survival rate are greatly improved.

The application provides a black fungus cultivation substrate using agricultural wastes and a preparation method thereof, and relates to the technical field of edible mushroom cultivation.The black fungus cultivation substrate using agricultural wastes and the preparation method thereof comprise the following steps: removing impurities and drying corn cobs, bean straws, peanut shells and rice husks, and crushing and screening them respectively.The black fungus cultivation substrate using agricultural wastes and the preparation method thereof are prepared by compounding agricultural wastes such as corn cob powder, bean straw powder, peanut shell powder and rice husk, and combining components such as wheat bran, soybean meal, gypsum powder, quicklime and superphosphoric acid calcium, so that a cultivation substrate suitable for the growth of black fungus is constructed.Meanwhile, by controlling the water content, pH value and carbon-nitrogen ratio of the substrate, the unity of the resource utilization of agricultural wastes and the formula optimization of the black fungus cultivation substrate is realized, which is beneficial to reducing the cost of cultivation raw materials, reducing the dependence on traditional wooden raw materials, and providing a more suitable nutrient and physicochemical environment for the growth of black fungus mycelium.

This invention discloses a method and system for detecting superficial fungi using microscopic images based on the YOLO model. The method involves collecting and annotating microscopic images of clinical superficial fungal disease samples. The annotated images are then classified and input into deep learning-based target detection models (including SSD-VGG16, Faster R-CNN, YOLOv5, YOLOv8, and YOLOv11 models) for training and testing. The system adjusts the Intersection over Union (IOU) threshold and evaluates the effectiveness of the confidence aggregation strategy. After preliminary performance evaluation, the best-performing model, YOLOv11, is optimized. The IOU threshold is optimized, and the system evaluates thresholds within the range of 0.25-0.75. Image-level confidence analysis is performed to evaluate the aggregation strategy. The YOLOv11 model of this invention performs optimally at 400× magnification, with an F1 score of 86.60% for hyphae detection and 95.90% for spore detection.

There is provided a microbial composition comprising Paenibacillus cineris and Micrococcus sp. for enhancing the inoculation rate of T. matsutake mycelium in trees, as well as a use thereof. The microbial composition according to the present invention significantly increases the inoculation rate of T. matsutake mycelium in trees. Furthermore, the composition does not inhibit the growth of T. matsutake mycelium and effectively suppresses harmful microorganisms that interfere with mycelium growth. The microbial composition of the present invention also remarkably improves the survival rate of T. matsutake-inoculated seedlings. According to the method for producing T. matsutake mycelium-inoculated seedlings of the present invention, seedlings with a high inoculation rate of T. matsutake mycelium and a high survival rate can be mass-produced and supplied in large quantities to mushroom cultivators.

A system and method for making a biopolymer-based fungal mat is described. The system includes a plurality of fresh fungal materials, a mass balance, a liquid chemical solution and a vacuum tumbler drum. The method comprises the steps of harvesting the plurality of fresh mycelium material and marking them for identification, then weighing the initial mass of the mycelium materials is carried out. The liquid chemical solution is decanted into the vacuum tumbler drum distributed with the mycelium material. Thereafter, vacuum is applied to the vacuum tumbler drum and the drum is rotated to ensure thorough mixing and refreshing of the liquid chemical solution at the mycelium surface. The process of vacuuming and rotating the drum is repeated and the fungal mat is formed. The fungal mat is removed from the vacuum tumbler drum for draining away surface moisture thereon to provide a dried fungal mat.