3883 results about "Hypha" patented technology

The invention discloses a mushroom cultivating method utilizing residues of sophora flower buds after extraction of rutins. The mushroom cultivating method utilizing the residues of the sophora flower buds after the extraction of the rutins comprises processing burdening, namely weighing cultivation raw materials which comprise, based on the weight percent, cotton seed hull of 42-60%, the residues of the sophora flower buds after the extraction of the rutins of 28-40%, bran of 10-20%, gypsum of 0.6-2%, calcium superphosphate of 0.3-1%, carbamide of 0.2-1% and sugar of 0.8-2% and adding water into the cultivation raw materials after uniform mix of the cultivation raw materials to enable water content of the cultivation raw materials to achieve 55-65%; bagging and sterilizing after the burdening processing; inoculating and cultivating hypha; building a shed and earthing up; coloring; and managing fruiting. The mushroom cultivating method utilizing the residues of the sophora flower buds after the extraction of the rutins has the advantages of being simple in operation, capable of cultivating the mushroom by adding small amount of nutritional ingredients, quick in spawn runing, low in production cost and capable of effectively utilizing by-product residues in phytoextraction industries in a maximum limit and achieving sustainable development.

The invention discloses two toadstool natural culturing methods belonging to one general invention conception. The first method comprises the following steps of mixing toadstool cultivated species with toadstool culturing materials and soil evenly, forming the mixture of the toadstool cultivated species, culturing materials and soil, sowing the mixture containing the toadstool cultivated species in a land and covering with soil with the thickness of 0.5cm-2.5cm; the toadstool culturing materials comprise herbaceous plant in grass family, crop straws, bran and KH2PO4, MgSO4, urea and other nutrient substances. The second method is as follows: hole sowing the toadstool cultivated species in the land directly and covering with soil with the thickness of 0.5cm-2.5cm. For the above two methods, at least one time of nutrition supplementation is carried out when mycelial is mature, and the nutrient supplementary solution is prepared by grass ash leachate, KH2PO4, MgSO4 and urea.

This invention relates to a manual culture method of selenium rich Cordyceps militaris and the application of fruit body. Firstly, the harvest wild bacterium of the Cordyceps militaris is purified normally, and the liquid strain which is produced by the purified strain is inoculated into the sterile incubation medium the content of selenium in which is rich. It is processed natural infection under the suitable situation and cultured continuously until the fruit body of Cordyceps militaris grows out. Otherwise the purified strain is produced into the liquid strain which is inoculated into the sterile incubation medium with frozen Cordyceps militaris powders. It is cultured continuously until the fruit body and hypha are filled full on the medium, spraying the selenium nutrient liquid until the Cordyceps militaris fruit body grows out. This technology decreases the regression of the strain and the decline of pharmacologic activity, and the operation is simple, the strain is stable, and the fruit body not only has the effective nutrient of Cordyceps militaris, but also it can hold the activity of the organic selenium long, the edible way is conveniently, and it is absolute no poison and the pharmacologic efficiency is notable.

The invention discloses a method for culturing edible fungi, which includes the following steps: culturing the mother culture in larger scale to prepare liquid culture spawn, introducing the liquid culture spawn together with culture medium which needs to be sterilized separately to cultivating material for thick solid ventilated culturing, when the cultivating material is filled with hypha, briquetting and shaping the cultivating material, and sending the briquetted cultivating material to a mushroom house to produce mushroom. The method is environment-friendly, energy-saving, short in production period, low in pollution rate and small in work intensity.

The invention discloses a production method for planting agaricus bisporus by using mushroom residues of pleurotus eryngii. The agaricus bisporus consists of the following substances in percentage by weight: 40 to 60 percent of mushroom residues, 20 to 40 percent of corn straws, 10 to 30 percent of cow dung, 1 to 3 percent of urea, 1 to 3 percent of lime and 0.5 to 2.5 percent of calcium superphosphate. In the production method for planting agaricus bisporus by using the mushroom residues of pleurotus eryngii, the routine production process for culturing agaricus bisporus is adopted, wherein the process comprises the following steps of: pre-wetting raw materials, piling the materials, turning over the piles, placing and laying the piled materials on a frame, fermenting the materials, seeding the materials, mycelium culture, earthing, taking out and managing the agaricus bisporus and harvesting fruit bodies. Because the mushroom residues for producing pleurotus eryngii are recycled to serve as the material for planting agaricus bisporus, environmental pollution is avoided and the aim of clean growth and environmental protection is fulfilled.

The invention discloses a stropharia rugoso-annulata cultivation method. The method comprises the following steps of season arrangement, cultivation material selection, site selection, material collection, cultivation material treatment, sowing and mycelium cultivation, spawn running period management, earthing method and management after earthing, fruiting period management, harvesting, pest and disease control and processing. The method has the advantages that the stropharia rugoso-annulata cultivation method fully utilizes the straw resource, the specific steps and conditions of cultivation are particularly limited, and the yield of stropharia rugoso-annulata can be effectively increased; meanwhile, by adjusting the cultivation proportion of different kinds of straw, growth and development of the stropharia rugoso-annulata can be effectively controlled, the stropharia rugoso-annulata is high in yield, excellent in quality and capable of adapting to the market requirement.

The invention provides a method of producing worm grass lotus seeds by virtue of solid state fermentation of lotus seeds. The method comprises the following steps: on the basis of utilizing the steamed lotus seeds as a worm grass solid state fermentation culture medium, inoculating 3-8ml worm grass liquid strain to per 100 g of the lotus seeds; culturing for 10-30 days at the temperature of 5-30 DEG C and the humidity of 60-90% in dark, namely obtaining worm grass lotus seeds on which hyphae are overgrown; and culturing the worm grass lotus seeds on which the hyphae are overgrown at the temperature of 5-30 DEG C and the humidity of 80-95% for 10-30 days with the illumination of 8-15 hours per day, thus obtaining the worm grass lotus seeds on which worm grass sporocarps are grown. The invention provides a new worm grass source and a new lotus seed health food. According to the invention, the medical effect of worm grass and the nutrition of the lotus seeds are organically combined, so that the product has various nutrient components, is especially rich in active components such as cordycepin, a cordycepic acid, a worm grass polyose and the like and has high edible and medical values; and the worm grass lotus seed fungus preparation process is simple and can easily realize large-scale production, popularization and implementation.

The invention relates to a production method for a biomass packing material. The production method includes the steps that culture raw materials such as corn straw and wheat straw are evenly mixed and the sterilized at a high-temperature; a specific fungus strain is inoculated after cooling is conducted; then, the inoculated culture raw materials are loaded into a sterile packing mold and cultured for several days in a sterile environment; and then the culture raw materials are treated through drying and water removing under the normal-temperature conditions, and the biomass packing material is obtained. According to the production method, agricultural and sideline products such as the corn straw and the wheat straw are used as the raw materials, the characteristics that fungus hyphae grow rapidly, and hypha bodies are high in twisting force are utilized so that the crop straw can be converted into the biomass packing material, and the packing material has the beneficial effects of being environment-friendly, ecological and low in weight and cost; comprehensive recycling of the agricultural and sideline products is achieved, an existing foam packing material can also be replaced, good elasticity and buffering performance are achieved, the degrading performance is good, and environment pollution is relieved; and the biomass packing material is an ideal environment-friendly biomass packing material.

The invention discloses a method for culturing selenium-enriched Cordyceps militaris, which is to increase the selenium content in a culture medium to 50mg/L by using organic selenium yeasts instead of inorganic seleninic acid and sodium selenite, which infect the growth of hyphae, so as to ensure the selenium content in the cultured selenium-enriched Cordyceps militaris reaches 35 to 50mg/L. In the invention, the organic selenium yeasts which exert the lest inhibition effect on the growth of the Cordyceps militaris are adopted to increase the selenium content in the culture medium to 50mg/l, the selenium content in the cultured selenium-enriched Cordyceps militaris reaches 35 to 50mg/kg, which is 2 times higher than that of the Cordyceps militaris cultured in a culture medium added with inorganic selenium, and the yield of Cordyceps militaris is high.

The invention discloses a Grifola frondosa strain for producing polysaccharide with a composite raw material of rice bran and wheat bran, which belongs to the technical field of microbial application technology and food biology. The Grifola frondosa strain JSU10 is preserved in China general microbiological culture collection center (CGMCC) in October 8th, 2010 with the CGMCC No. 4179 and is identified to be Grifolasp. The invention improves the positive mutation rate of the Grifola frondosa strain for rapidly growing and producing Grifola frondosa polysaccharide on a composite culture medium of rice bran and wheat bran by composite mutagenesis of ultraviolet rays and microwave to obtain a high-yield strain; the strain and the original strain are respectively a liquid fermentation rice bran and wheat bran composite culture medium; and hypha dry weight and polysaccharide of the mutated strain are respectively improved by 39.24 percent and 42.58 percent compared with the original strain.

The invention provides a cordyceps militaris fruiting body culturing method used yellow meal worm pupae as host. The fruiting body is cultured by using cordyceps militaris link to infect yellow meal worm. Its features are as follows: yellow meal worm is yellow brown or chocolate brown; fruiting body is bar type and orange yellow. The culturing method includes bacteria species preparing, yellow meal worm inoculating infecting, and out grass. The formed product has multi nutrient content and effect active constituent, and uses as foods. The culturing method is simple, effective, and reliable. And the cost is low.

The present invention provides a formula of high-yield flammulina velutipes culture medium and a producing technique thereof. The invention relates to the technical filed of biological strain, and specially to the formula of high-yield flammulina velutipes culture medium and producing technique thereof. The corncob is used as main ingredient for replacing fir sawdust. The invention adopts 40-45% of corncob, 10-12% of bagasse, 25-30% of rice bran, 8-10% of bran, 4-6% of soybean hull and 2-3% of corn flour as the raw materials of flammulina velutipes culture medium. The method comprises the techniques of dry mixing, wet mixing, etc. The pH value is controlled between 7.0 and 7.5. The cultured flammulina velutipes has the advantages of strong activity of mycelium, high production volume, ordered mushroom generation, excellent commodity valve, ordered growing of sporphore, uniform magnitude of bonnet, white color, and no infection of foreign fungus. The unit production obtains 286-293grams/bottle. The dry object of each bottle is 260grams. The biological transformation efficiency reaches up to 110-113% and is increased for 30-40% compared with the common formula. The economic benefit is excellent.

The invention discloses a bagged mushroom production method which comprises the steps of strain making, nutrient preparation, bagging, sterilization, inoculation, mycelium culture, coloring, inducement to primordium of mushroom and harvesting. The method is characterized in that 1, in the nutrient preparation step, 78% of miscellaneous tree sawdust, 20% of wheat bran, 1% of sucrose and 1% of gypsum in percentage by weight are evenly mixed, then 55-58% of water is added, and blending is performed; 2, the nutrient is put in barrel bags within 4 hours; 3, the bags are piled in the shape of a well and are sterilized; 4, when the bags are cooled to 25-28 DEG C, inoculation is performed; 5, the mushroom bags are transferred into a mushroom growth room of 20-25 DEG C and subjected to mycelium culture, and puncturing ventilation is performed twice in the mycelium culture period; and 6, in the mushroom growth management period, artificially-aided inducement to primordium of mushroom, dry stimulation and other treatment operations are performed. According to the invention, large-scale bagged mushroom production can be realized, the mushroom survival rate is high, the deformed mushroom defective rate is below 5%, the cost can be reduced and the product quality is high.

The invention belongs to the field of microbial application technology and food biotechnology, and discloses a ganoderma lucidum strain used for producing polysaccharides by complete feed liquid fermentation of rice bran and wheat bran. Ganoderma lucidum JSU6161314 is stored in China Center for Type Culture Collection (CCTCC) on 25th, June, 2013, preservation number is CCTCC M2013287, and the bacterial strain is named as Ganoderma lucidum CCTCC M2013287. According to a method disclosed in a drawing of the invention, the novel bacterial strain, which is capable of realizing high yield of ganoderma lucidum polysaccharides, is obtained by modifying ganoderma lucidum CFCC6043. The novel bacterial strain is used for rapid fermentation in a rice bran and wheat bran complete feed liquid culture medium and high yield of ganoderma lucidum polysaccharides. After fermentation, hypha dry weight and hypha polysaccharide yield increase 23.0% and 45% respectively compared with that of the original strain.

The invention discloses a large-scale cultivation method and a quality detection method of cordyceps militaris fruit bodies, comprising the steps of: (1) screening spawn; (2) preparing a rice solid culture medium (40-55 parts of rice and 45-60 parts of nutrient solution in every 100 parts by weight) and killing bacteria; (3) preparing liquid spawn: activating the spawn, inoculating the spawn into a liquid culture medium, conducting static culture for 24 hours and performing suspension culture on a shaking table; (4) culturing fruit bodies: inoculating the liquid spawn on the solid culture medium for dark culture for 6 days at the temperature of 20 DEG C until the surface of the medium is covered by mycelium; placing the well grown mycelium under incandescence with light intensity of 100 to 300 lux, wherein during a primordium growth phase, illumination is performed for 20-24 hours per day at the temperature of 20-24 DEG C; and during a fruit bodies growth phase, illumination is performed for 8-12 hours per day at the temperature of 18-20 DEG C and the fruit bodies are harvested 5 to 8 days after mature. The cordyceps militaris fruit bodies cultured by the method have the advantages of high yield and high content of active ingredients. Batches of cordyceps militaris fruit bodies have fingerprints with a similarity value greater than 0.950, indicating stable and controllable quality.

The invention provides a method for cultivating glossy ganoderma in a thick bamboo tube. In the cultivation of the glossy ganoderma, the thick bamboo tube is adopted to replace a fungi package; one end of the thick bamboo tube is open and the other end is reserved as the bottom; prepared culture medium is filled in the thick bamboo tube; after the opening is sealed, the sterilization and inoculation are performed according to convention; the thick bamboo tube is transferred into a culture room for hypha culture; after the hypha grows in the whole thick bamboo tube, the hypha is covered by earth to be cultivated in a field; the thick bamboo tube adopts annual bamboo or biennial bamboo, the bamboo is cut into the thick bamboo tube with one open end and one reserved bottom according to bamboo sections, and the thick bamboo tube is filled with the glossy ganoderma culture medium. The invention adopts the natural thick bamboo tube to replace polyethylene and polypropylene packages, and the thick bamboo tube has wide resource, can be repeatedly used, and is favorable for protection of ecological environment and recycling of resource; the cultivation process is simplified, prevents the pollution of fungi rods and plant diseases and insect pests, and improves the output and quality of the glossy ganoderma; and the glossy ganoderma also has the health care efficacy of the bamboo, and obvious economic benefit, and is suitable for promotion and application on a large scale.

A manufacturing method for cordyceps sinensis (Berk.) sacc polysaccharide relates to the technical field of food microorganism application, and comprises the following steps: weighing raw materials, wherein rice bran is 1-3 g per 100 ml, bran is 1-3 g per 100 ml, potassium dihydrogen phosphate is 0.09-0.18 g per 100 ml, and magnesium sulphate is 0.04-0.08 g per 100 ml; material filling with natural pH after adding required water, and sterilizing and cooking at the temperature of 121 DEG C for 30 min; inoculating cordyceps sinensis (Berk.) sacc CCTCCM2013285 liquid seed after cooling, wherein the inoculation amount is 8-10%, the fermentation temperature is 23-28 DEG C, and the fermentation time is 4-7 d; respectively obtaining exopolysaccharides and mycelia polysaccharide after conducting deproteinization, alcohol precipitation, centrifugal separation, and vacuum drying on centrifugal separation liquid extracted through breaking cell wall hot water of fermentation material centrifugal separation liquid and hypha. The method better realizes the purpose that rice bran and bran are efficiently and high-valuedly transformed to cordyceps sinensis (Berk.) sacc polysaccharide after being subjected to liquid state fermentation.