610 results about "Hypha" patented technology

The invention discloses an intelligent nutrient circulation regulation and control method for a morchella esculenta fruit forest intercropping system based on the Internet of Things, particularly relates to the technical field of intelligent agriculture, and is used for solving the problem of symbiosis imbalance of hyphae and fruit trees caused by nutrient regulation and control lag and static threshold setting in the prior art. Hypha density, electric signal dominant frequency and root system absorption rate data are collected in real time through a multispectral impedance sensor and an ion selective electrode array, and a space-time aligned dynamic matrix is constructed; dynamically updating a nutrient balance threshold value based on a real-time ratio of a hypha metabolism rate to an absorption rate, and generating a nutrient transfer priority by combining collaborative analysis of a hypha transfer direction included angle and an electric signal main frequency offset gradient; a continuous transfer path is identified through a region growing algorithm, water and fertilizer amounts are distributed according to priorities, and a local area instruction is generated; and inverting the equipment response delay by using the high-frequency pulse signal, synchronously adjusting the execution frequency and updating the dynamic matrix and the threshold parameter, thereby realizing the dynamic adaptation and autonomous optimization of the nutrient supply and demand of the intercropping system.

The invention relates to application of turmeric rhizome extract emulsifiable concentrate in preventing and treating corn leaf spot and tomato gray mold. Compared with the prior art, the turmeric rhizome extract missible oil disclosed by the invention contains an effective substance aromatic turmerone, and the aromatic turmerone can reduce the spore germination rate and sporulation quantity of pathogenic bacteria, increase the permeability of hypha cell membranes and cause overflow of contents, so that normal growth of hyphae is inhibited, and an antibacterial effect is achieved. Aromatic turmerone acts on plants, so that plant cells generate more polyphenol and flavonoid substances, and the immunity of the plants is improved. Pharmacodynamic test results in fields in two fields show that the 6.5% turmeric rhizome extract emulsifiable concentrate can be used for stably and effectively preventing and treating corn northern leaf blight and tomato gray mold after being diluted by 150-300 times, and also has a yield increasing effect.

The invention discloses a mother strain culture medium for boletus xylophilus as well as a preparation method and application of the mother strain culture medium, and belongs to the technical field of microbial culture. The preservation number of the strain JSJ-Bx1 of the boletus sinensis mother strain is CGMCC (China General Microbiological Culture Collection Center) No.42007, and the strain JSJ-Bx1 is preserved in the China General Microbiological Culture Collection Center; the culture medium is prepared from 10-30 g of a carbon source, 2-6 g of a nitrogen source, 1-3 g of inorganic salt, 0.1-0.3 g of a growth factor suitable for the culture medium, 20 g of agar and 1 L of water, and the pH of the culture medium is 4-6. By the adoption of the culture medium, hyphae of the boletus sinensis mother strain grow fast, grow vigorously and are dense, the edges of bacterial colonies are neat, hypha degeneration phenomena such as pigment and water spitting are avoided, and the mother strain culture growth cycle is greatly shortened.

The invention discloses a multi-factor coupling ganoderma lucidum environment digital twinborn regulation and control method oriented to high-yield requirements, particularly relates to the technical field of intelligent cultivation of edible mushrooms, and is used for solving the problems of equipment action oscillation and recessive physiological stress caused by physiological response hysteresis of ganoderma lucidum in an existing environment regulation and control system. Carbon dioxide concentration and illumination intensity predicted values are generated in real time by constructing ganoderma lucidum growth digital twins, and an oxidative stress mark is activated by combining hypha respiratory entropy matching degree analysis and an active oxygen accumulation curve; when the matching degree exceeds a threshold value and oxidative stress exists, a metabolic overload signal is generated, and whether the carbon dioxide predicted value is in a physiological hysteresis interval or not is judged; delay compensation and metabolic overload suppression coefficient adjustment are performed on the carbon dioxide predicted value in the delay interval; and finally, inputting the compensated carbon dioxide value and the illumination prediction value into an environment factor coordination rule base, generating a ventilation opening degree and light supplementing power instruction to drive execution equipment, and ensuring the morphological development stability of the sporocarp and the synthesis efficiency of the active component.

The invention relates to the technical field of agricultural micro-ecological application, and particularly discloses a composite combined inoculation method using arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria, which comprises the following steps: selecting corn plant seeds without plant diseases and insect pests; a corn planting land is randomly divided into land parcels with the area of 6 m < 2 > (2 m * 3 m), a buffer zone of 1.0 m is arranged between every two adjacent land parcels, and the cultivation mode is flat cropping; the method comprises the following steps: planting arbuscular mycorrhizal fungi inoculum comprising mycorrhiza, dry sand containing spores and ectotrophic hyphae and corn seeds in soil by using a seeder during corn sowing, and after the corn is planted for 30 days, inoculating the plant growth-promoting rhizobacteria inoculum into plants and soil by using a flood irrigation root irrigation method, when the arbuscular mycorrhizal fungi and the plant growth-promoting rhizobacteria are inoculated together, the corn rhizosphere microbial community can be changed, the richness and diversity of fungi and bacterial communities in soil are increased, and the relative abundance of plant pathogens in the soil is reduced.

The invention is suitable for the technical field of ganoderma lucidum cultivation, and provides a ganoderma tsugae and leukoderma lucidum cultivation and planting device and method.The ganoderma tsugae and leukoderma lucidum cultivation and planting device comprises a cultivation box, and an isolation frame is fixedly installed in the cultivation box so that the interior of the cultivation box can be isolated into a plurality of independent cultivation cavities; a plurality of pick-and-place openings are formed in one side of the cultivation box and correspond to the multiple cultivation cavities respectively, cultivation drawers are arranged in the multiple cultivation cavities in a drawing mode and used for containing culture media to cultivate hyphae, and the cultivation drawers can pass through the pick-and-place openings. According to the ganoderma tsugae and leukoderma lucidum cultivation and planting device and method, the cultivation box is divided into the multiple cultivation cavities through the isolation frames, each cultivation cavity is provided with an independent taking and placing opening, physical isolation of a hypha cultivation environment is achieved, cross contamination is avoided, the hoisting plate is fixed to the lower portion of the sliding piece, and a camera and a sterilizing lamp are carried. And the hyphae in the cultivation drawer can be monitored in real time and dynamically disinfected.

The invention provides a coal gangue-based soil matrix as well as a preparation method and application thereof, and belongs to the technical field of ecological restoration. According to the invention, the coal gangue with continuous gradation is adopted, so that the coal gangue-based soil matrix has the particle size distribution of natural soil; urea is added to adjust the carbon-nitrogen ratio of the coal gangue and the biomass waste, and a microbial agent is added for pre-curing, so that the microbial activity is improved, complete decomposition of organic matters is promoted, and formation of water-stable aggregates is promoted; multi-layer root plants are planted together and inoculated with fungi, formation of water-stable aggregates is promoted through root interpenetration and a hypha network, meanwhile, the nutrient circulation efficiency is improved together with root exudates and microbial metabolism, and nutrient release is more balanced. Through multi-dimensional symbiotic regulation and control, the coal gangue-based soil matrix has particle size distribution of natural soil, nutrient balance, high water-stable aggregate content and organic matter content, and is more beneficial to ecological restoration.

The invention relates to the technical field of biocontrol strains for crops, in particular to bacillus velezensis JR1A and application thereof to inhibition of garlic pathogenic fungi. The bacillus velezensis JR1A is preserved in the China General Microbiological Culture Collection Center on June 30, 2025, and the preservation number of the bacillus velezensis JR1A is CGMCC No.35059. The bacillus velezensis JR1A is named as bacillus velezensis JR1A. The JR1A inhibits mycelial growth and spore production of pathogenic fungi of common garlic diseases, has an excellent biocontrol effect and a certain growth promoting function, can reduce the relative abundance of pathogenic fungi in garlic crop rotation soil, and reduces the occurrence of related garlic diseases.

The invention discloses an artificial cultivation method of xylaria fungus sclerotium, and relates to the field of sclerotium cultivation. The method comprises the following steps: collecting xylaria producing sclerotia, obtaining spores by adopting a spore separation method or obtaining core tissues by adopting a tissue separation method, inoculating the spores or the core tissues on an enriched PDA culture medium to be cultured into bacterial colonies, selecting hyphae with vigorous growth vigor at the edges of the bacterial colonies, and performing purification culture until a mother strain only containing a single strain is obtained; performing gene sequencing to identify the strain; propagating the mother strain to obtain an original strain, inoculating the original strain into a sterilized solid culture medium, culturing in a dark place to obtain a cultivated strain, placing the cultivated strain on soil, reserving a space for sclerotium development around the cultivated strain, culturing in the dark place for 33-90 days, and harvesting the sclerotium. The xylaria sclerotium with single germplasm can be cultivated on a large scale, and the problems of wild resource shortage and germplasm mixing are effectively solved.

The invention belongs to the technical field of water treatment, and particularly provides a nitrogen-oxygen co-doped hypha / spruce composite adsorption material and a copper-containing sewage treatment method. According to the invention, the copper-containing sewage is pretreated by using the solid fermentation product, and then the copper-containing sewage is post-treated by using the nitrogen-oxygen co-doped hypha / spruce composite adsorption material prepared under specific conditions; wherein in the pretreatment stage, microbial metabolites in a solid fermentation product preliminarily capture free copper ions (Cu < 2 + >) through complexation, the toxicity of the sewage is reduced, and meanwhile, the copper ions are partially converted into a form which is easier to adsorb. In the post-treatment stage, the nitrogen-oxygen co-doped hypha / spruce composite adsorption material plays a key role, and efficient interception and adsorption of copper ions are achieved. In conclusion, the treatment method for the copper-containing sewage comprises a pretreatment stage and a post-treatment stage, the two stages are in synergistic interaction, and finally the removal rate of the heavy metal copper can be effectively increased.

The invention discloses a cordyceps sinensis intelligent bionic cultivation control system and method based on the Internet of Things, and the method comprises the steps: collecting the temperature and humidity, gas concentration, illumination intensity and larva behavior data of a cordyceps sinensis matrix through a sensor network, and carrying out the fusion through an edge calculation gateway, and generating a dynamic environment map; constructing a plateau ecological digital twinborn model based on the dynamic environment map in combination with historical meteorological data and a host-strain interaction rule, and generating a staged control time sequence; based on a staged control time sequence, temperature and humidity coupling compensation is carried out through a feedforward-feedback composite algorithm, and actuator linkage is adjusted through fuzzy PID; aI vision is used for identifying a larva infection window period and accurate spraying is carried out, and hypha infection is monitored in combination with a near infrared spectrum to dynamically adjust the CO2 concentration; and constructing an LSTM (Long Short Term Memory) model to predict the growth risk in combination with a dynamic environment map and insect body monitoring data, and actively intervening in an abnormal environment. According to the invention, high-precision, dynamic and intelligent regulation and control of the growth environment of cordyceps sinensis are realized.

The invention belongs to the technical field of genetic engineering of edible mushrooms, and particularly relates to a construction method of an efficient and stable genetic transformation system of flammulina velutipes. The construction method comprises the following steps: culturing in an MM culture medium by taking a flammulina velutipes strain 0747 as a receptor; the pFgnpt vector is converted into agrobacterium AGL1 (AGL1); inoculating hyphae into an IM liquid culture medium containing acetosyringone and MES, mixing with agrobacterium tumefaciens, culturing, and transferring the hyphae to an IM solid flat plate paved with filter paper; hyphae are transferred to a PDA resistance plate containing G418 and cephalosporin for resistance screening, and an efficient and stable flammulina velutipes genetic transformation system is obtained. The new selection marker G418 is developed and applied, limitation of a traditional selection marker is broken, more choices are provided for flammulina velutipes genetic transformation, a new thought is provided for genetic transformation research of other edible mushrooms, and the application field of an agrobacterium tumefaciens-mediated transformation technology is expanded.

The invention relates to the technical field of image recognition, in particular to a horticultural product fungal disease image recognition system and method, and the system comprises an image collection module, an image segmentation module, an anomaly detection module, a feature extraction module, a disease recognition module and a decision output module. In the prior art, the intensity of a light supplementing lamp is manually adjusted, and instantly changing illumination conditions (such as cloud layer shielding and tree shadow shaking) are difficult to deal with in an open-air plantation, so that leaf scab details are often submerged by strong light or dim light is blurred; according to the scheme, a closed-loop linkage system of ambient light perception and an annular LED is innovatively deployed, and the light supplementing intensity is reversely adjusted in a millisecond level by tracking ambient brightness in real time, so that purple gray hyphae of downy mildew in mist in the morning and anthracnose black spots under the noon burning sun present completely consistent color saturation and texture definition in imaging; and the problem of misdiagnosis caused by illumination fluctuation in the traditional method is thoroughly solved.

The invention discloses a hypha network reinforced ecological slope protection structure and a construction method.The hypha network reinforced ecological slope protection structure comprises a plurality of ecological soil nails arranged on a side slope, and each ecological soil nail comprises a soil nail head located outside the side slope; a hollow shell extending into the slope is arranged at one end of the soil nail head, a grouting hole communicated with the hollow shell is formed in the soil nail head, and a plurality of leakage holes are formed in the surface of the hollow shell; a plurality of ecological panels are arranged on the inclined face of the side slope, and the ecological soil nails are filled with grouting liquid with hypha spores. An assembly type structure frame is formed by inserting and matching the ecological panels and the ecological soil nails, grouting liquid with spore bags is injected, after the spore bags are activated, mycelium is combined in a winding mode in the growth process, and a high-ductility biological fiber network structure is constructed between soil particles and components. And the structural integrity and the shear resistance and disturbance resistance of the side slope are improved.

The invention provides a fluorescence image recognition system for detecting fungi based on a deep learning algorithm. The fluorescence image recognition system comprises an image acquisition module, a single spore and hypha recognition module and a hypha group recognition module, the image acquisition module is used for acquiring a target fluorescence image; and the single spore and hypha identification module and the hypha mass identification module are used for inputting the target fluorescence image into a pre-established deep learning algorithm model, identifying the single spore or hypha and the hypha mass through the pre-established deep learning algorithm model, and outputting a fungus negative or positive result according to an identification result. Compared with the prior art, after the target fluorescence image is obtained by the image acquisition module, the target fluorescence image is input into the pre-established deep learning algorithm model for automatic analysis, and analysis of spores / hyphae and hypha masses which are greatly different in form is divided into two modules for processing; and spores, hyphae and hypha masses can be identified and distinguished more accurately.

The invention relates to a cavern environment-based tricholoma matsutake artificial cultivation method, and relates to the technical field of edible fungus cultivation, and the method comprises the following steps: mushroom stick preparation: mixing pine sawdust, corncob, bran, rice bran and an additive, adding water, bagging, sterilizing with high-pressure steam or normal-pressure steam, and inoculating tricholoma matsutake strains into each bag; environment construction: installing temperature and humidity regulation and light scattering equipment in cave depots, natural caves and air-raid shelters with the soil layer thickness of more than 50 m, the constant temperature of 10-20 DEG C and the humidity of 70-90%; in the hypha stage, the temperature is controlled to be 15-18 DEG C, the humidity is controlled to be 70-80%, and culture is conducted for 40-60 days; in the fruiting stage, the temperature is lowered to 12-16 DEG C in a stepped mode, the humidity is increased to 85-90%, and harvesting is conducted after fruiting bodies are induced to be formed. And recycling resources: crushing the waste mushroom sticks, and mixing the crushed waste mushroom sticks with soil according to a ratio of 1: 3 to prepare a fertilizer or mixing 10-30% of a new material for reuse. The produced tricholoma matsutake has the tricholoma matsutake alcohol content of 77.21 mg / kg and the polysaccharide content of 2.67%, the active components of the tricholoma matsutake are equal to those of wild tricholoma matsutake, the hypha germination rate is greater than or equal to 95%, the growth period is shortened to 40-60 days, and the raw material consumption is reduced by

The invention belongs to the technical field of mushroom planting, and particularly relates to a pleurotus nebrodensis planting method which comprises the following steps: mixing raw materials of a pleurotus nebrodensis culture medium in parts by weight: 70-85 parts of cottonseed hulls, 5-15 parts of bran and 3-8 parts of corn flour, adding water to enable the water content of the culture medium to be 60%-65%, and uniformly stirring; filling the culture medium into a fungus bag to prepare a cultivation fungus stick; performing high-pressure sterilization on the cultivation mushroom sticks; inoculating the cultivation mushroom sticks in a sterile room; moving the cultivation mushroom sticks into a spawn running room to culture hyphae; after the bags are full of hyphae in the cultivation mushroom sticks, the cultivation mushroom sticks are moved into a growth chamber, buds are thinned after fruiting, and 1-2 mushroom bodies are reserved; after the mushroom bodies are mature, harvesting the mushroom bodies; according to the planting method, the quality and yield of pleurotus nebrodensis can be effectively improved, accumulation of toxins in the culture medium can be reduced, recycling of the culture medium is facilitated, and the production cost of farmer households is reduced.

The invention discloses marasmius albus DCY6478 as well as a culture medium and application thereof, and belongs to the technical field of microorganisms. The invention discloses a Marasmilla sp. DCY6478 strain, and the preservation number of the Marasmilla sp. DCY6478 strain is GDMCC (China General Microbiological Culture Collection Center) No.65883. The Marasmilla sp. Experimental verification results show that the culture medium formula most suitable for growth of the white marasmius is prepared from the following components in percentage by weight: 40% of apple sawdust, 36% of corncob, 14% of cottonseed hull, 1% of lime and 1% of gypsum; the fermentation liquor is verified to have an inhibition effect on a plurality of pathogenic bacteria; the DPPH free radical scavenging capacity of the fermented hyphae is verified, the superoxide dismutase activity and catalase activity are both higher than those of sporocarp, and the fermented hyphae can be used as a natural antioxidant source; it is verified that fermented hyphae or sporocarp of the strain have the capacity of secreting amino acid and polysaccharide and producing crude protein, and a new raw material selection is provided for the industries of food, medicine and the like.

The invention discloses a hypha mineral composite bionic sand stabilization unit body and a preparation method thereof, and relates to the technical field of sand stabilization, the interior of the unit body is provided with a bionic three-dimensional porous skeleton structure, the unit body comprises a porous core layer, a porous core layer and a porous core layer, the porous core layer takes cleaning sand grains with the particle size of 0.1-2 mm as a sand-based carrier; uniformly mixing and curing with organic nutrient substances, mineral precursors and strain spores; the composite reinforcing layer is formed by interweaving and compounding a mycelium network formed by germination and growth of strain spores, mineral crystals generated by in-situ mineralization induced by metabolic activity of hyphae and a plant fiber reinforcing material which is externally added and embedded into the pores in the pores of the porous core layer; according to the hypha mineral compounded bionic sand stabilization unit body and the preparation method thereof, mold pressing or 3D printing prefabrication is adopted, the structure is controllable, and standardized production and transportation are facilitated; and through a controllable fermentation and gradient dehumidification mineralization culture process, hyphae and minerals are ensured to be uniformly and fully compounded in pores, and the product quality is stable.

The invention relates to the technical field of biological control of plant diseases, in particular to application of a bacillus atrophaeus strain and a preparation thereof to control wheat gibberellic disease caused by gibberellic maize. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.29799, and the strain is classified and named as the bacillus atrophaeus strain HHQGTS13-1 (Bacillus atrophaeus HHQGTS13-1). The bacillus atrophaeus strain HHQGTS13-1 is wide in application range to natural environment conditions such as temperature and pH, and has the capability of antagonizing fusarium maydis when the temperature is 10-45 DEG C and the pH value is 4-10. The bacillus atrophaeus strain HHQGTS13-1 can be used for obviously inhibiting the growth of fusarium maydis hyphae and enabling the hyphae to be deformed; the antagonistic bacillus HHQGTS13-1 strain has an obvious inhibition effect on spore germination of fusarium maydis, the spore germination inhibition rate is 97.10%, the prevention and treatment effect of the antagonistic bacillus HHQGTS13-1 strain fermentation liquor after wheat is inoculated with fusarium maydis pathogenic bacteria is 63.43%, and the antagonistic bacillus HHQGTS13-1 strain has good potential of preventing and treating plant diseases caused by fusarium maydis.

A cultivation method for Morchella on patterned layer frames is provided. The method includes: (1) preparing and laying a culture substrate, and sowing; (2) supplementing an exogenous nutrient material; (3) mycelium culture; (4) primordium induction; (5) primordium differentiation; (6) management of a young mushroom period; (7) maturity management; and (8) erchao mushroom management. The method adopts a low temperature of (5-9)° C. to promote a transformation of an exogenous nutrition and adopts a low temperature of (2-4)° C. to induce a differentiation of primordium and other measures, to address a bottleneck of industrial development that cannot continuously supply fresh Morchella to the market, which is caused by some problems in the cultivation of Morchella, such as an unstable yield and seasonal limitation in the existing cultivation of Morchella. The method can carry out industrial facility cultivation for Morchella, and can obtain Morchella with stable yield and good quality.

This invention discloses a Chinese saprophytic bolete ( Buchwaldoboletus xylophilus This invention relates to the mother culture medium, its preparation method, and its application, belonging to the field of microbial culture technology. The strain JSJ-Bx1 of the *Boletus spp.* mother culture, with accession number CGMCC No. 42007, is deposited at the China General Microbiological Culture Collection Center. The culture medium composition is: 10–30 g carbon source, 2–6 g nitrogen source, 1–3 g inorganic salts, 0.1–0.3 g suitable growth factors, 20 g agar, and 1 L water, with a pH of 4–6. Using the culture medium of this invention, the mycelium of the *Boletus spp.* mother culture exhibits rapid, vigorous, and dense growth, with neat colony edges, and does not produce pigments or exhibit mycelial degeneration such as exudation, significantly shortening the mother culture growth cycle.

The invention provides a method and system for calculating the utilization rate of soil nitrogen under the action of arbuscular mycorrhiza, and relates to the technical field of calculation of the utilization rate of soil nitrogen under the action of arbuscular mycorrhiza. Soil nitrogen absorption amount data of an experimental group and a control group are collected, meanwhile, the total amount of externally input nitrogen is recorded, and related photosynthetic parameters of plants are measured; in addition, through an in-vitro mycelium culture experiment, mycelium nitrogen absorption rates under different nitrogen concentrations are measured, and a semi-saturation constant and a nitrogen effectiveness index are calculated, so that a carbon-nitrogen coupling factor is calculated, morphological parameters of mycelia are collected, and a relation equation of a pipe diameter and a transport rate is established; calculating the total flux of the hypha network, calculating the original nitrogen utilization rate in combination with the nitrogen absorption amount difference value of the experimental group and the control group and externally input nitrogen data, and introducing a transportation continuity coefficient for dynamic correction to finally obtain the soil nitrogen utilization rate of the target area.

The invention discloses a tricholoma matsutake mushroom, which is classified and named as tricholoma matsutake mushroom 11, and is preserved in China Center for Type Culture Collection on March 24, 2025 with the preservation number of CCTCC NO: M 2025577. The molecular marker identification method comprises culture and collection of hyphae, extraction of genome DNA, DNA fingerprint PCR amplification and electrophoresis detection of PCR products. According to the method, the tricholoma matsutake mushroom 11 which inherits the advantage of high thermotype of two parents, is good in hypha temperature tolerance and high in vigor, is similar to tricholoma matsutake in sporocarp shape and is not opened is obtained by carrying out crossbreeding of the mushrooms and screening after mushroom cultivation and fruiting, and the tricholoma matsutake mushroom 11 has good development and application prospects.

The invention belongs to the technical field of temperature control, and particularly relates to an intelligent temperature control method for an edible mushroom cultivation growth room, which comprises the following steps of: judging temperature abnormity by combining a comprehensive evaluation function of an instantaneous overrun proportion and a standard deviation, and determining a regulation priority according to the temperature abnormity, so that the conversion from average temperature control to abnormal region priority regulation is realized; a shelf two-dimensional temperature distribution diagram is constructed, gradient calculation is carried out, a high-temperature area and the orientation thereof are accurately positioned, the deflection direction and angle of a guide plate are directly controlled through coordinate transformation and nonlinear mapping, cold air flow preferentially flows to the high-temperature area, the problem of uneven horizontal and vertical temperature caused by a three-dimensional cultivation shelf is effectively solved, and the quality of the cultivation shelf is improved. Temperature regulation and control effect verification is intelligently fused with the mycelial growth speed conformity and the fruiting uniformity of the edible mushrooms, the growth conformity is quantified through image analysis, graded early warning is performed based on a secondary verification result, and a complete intelligent closed-loop control system is formed.

The invention belongs to the field of edible fungus culture, and particularly relates to a method for reducing disease occurrence and increasing the yield of dictyophora rubrovolvata. According to the method, before planting, a rotary cultivator is used for ploughing soil, wood vinegar is sprayed, temperature and humidity are controlled after hyphae are put on the soil, wood vinegar is sprayed in the growth stage of bamboo eggs, and temperature and humidity are controlled until harvesting, so that the occurrence of diseases is reduced, and the yield of dictyophora rubrovolvata is increased. The invention provides a method for reducing diseases and increasing the yield of dictyophora rubrovolvata through measures of improving the pH value of soil, preventing soil caking, stimulating rapid growth of hyphae, stimulating twisting of the hyphae, reducing diseases and insect pests and the like. By means of the method, use of pesticides in the dictyophora rubrovolvata planting process can be reduced, the yield of dictyophora rubrovolvata is increased, and dictyophora rubrovolvata diseases are reduced.

The invention discloses a selenium-rich pleurotus citrinopileatus cultivation fungus bag. The selenium-rich pleurotus citrinopileatus cultivation fungus bag is prepared from, by weight, 82%-84% of sawdust, 14%-16% of bran, 0.5%-1.5% of lime, 0.5%-1.5% of gypsum and 1 mg / kg-50 mg / kg of nano-selenium. A suitable microenvironment is provided for growth of hyphae through a stable base material structure, rapid planting of the hyphae is promoted, sufficient energy is provided for growth of the hyphae, and more nutrient substances are accumulated for development of sporocarps.

The invention relates to the technical field of intelligent monitoring, in particular to an intelligent monitoring method and system for tricholoma matsutake cultivation of an agricultural intelligent technology shelter. The method comprises the following steps: monitoring temperature data, humidity data, light environment data and gas concentration data corresponding to a tricholoma matsutake culture growth stage in real time in an agricultural intelligent science and technology shelter, and transmitting the data to a data processing center by using a wireless communication technology; carrying out growth stage classification and optimal environment screening on temperature data, humidity data, illumination environment data and gas concentration data corresponding to the tricholoma matsutake culture growth stage, and generating optimal tricholoma matsutake growth environment conditions corresponding to a hypha growth stage and a fruiting stage; and based on the optimal tricholoma matsutake growth environment conditions corresponding to the mycelium growth period and the fruiting period, performing tricholoma matsutake growth intelligent monitoring analysis in the corresponding growth stages in the tricholoma matsutake culture growth stages, and generating a corresponding tricholoma matsutake culture growth environment intelligent regulation and control decision. The method can provide suitable growth environment conditions for tricholoma matsutake.

The invention discloses a genetic transformation method of PEG (Polyethylene Glycol) mediated penicillium sclerotiorum strain protoplast and application of the genetic transformation method. The method comprises the following steps: (1) culturing penicillium sclerotiorum to produce spores, germinating hyphae, and collecting and cleaning fresh penicillium sclerotiorum hyphae; (2) compounding Lysing Enzymes, Yatase, cellulase and pectinase to prepare a protoplast enzymatic hydrolysate, and then adding the protoplast enzymatic hydrolysate into the penicillium sclerotiorum hypha for enzymolysis to obtain a penicillium sclerotiorum protoplast; and (3) adding plasmids to be transformed into the penicillium sclerotiorum protoplast, and carrying out PEG (Polyethylene Glycol) mediated transformation and resistance screening to obtain a penicillium sclerotiorum protoplast transformant. According to the method, the number and regeneration rate of the protoplast can be remarkably increased, and the enzymolysis time can be effectively shortened.

The invention relates to an application of carboxylated chitin or carboxylated chitosan in promoting insect-killing fungi to prevent and treat diseases and insect pests. Research finds that carboxylated chitin or carboxylated chitosan can significantly promote spore germination and hypha growth of insecticidal fungi, also can significantly enhance stress resistance of the insecticidal fungi, significantly enhance toxicity of the insecticidal fungi to pests, improve ultraviolet resistance and high temperature resistance of the insecticidal fungi, and improve the insecticidal activity of the insecticidal fungi. The invention also finds that the carboxylated chitin or chitosan can effectively inhibit the growth of Xanthomonas oryzae pv. Oryzae. Therefore, the carboxylated chitin or the carboxylated chitosan can be used for preparing a product for enhancing the insecticidal fungus effect and a product for preventing and treating the rice bacterial leaf blight, and the prevention and treatment of plant diseases and insect pests are realized. In addition, carboxylated chitin and carboxylated chitosan are derived from natural polymer materials, have the characteristics of safety, no toxicity, biodegradability, environmental friendliness and the like, are environment-friendly and economical, and have wide application prospects and market values.