3905 results about "Hypha" patented technology

The invention discloses a mushroom cultivating method utilizing residues of sophora flower buds after extraction of rutins. The mushroom cultivating method utilizing the residues of the sophora flower buds after the extraction of the rutins comprises processing burdening, namely weighing cultivation raw materials which comprise, based on the weight percent, cotton seed hull of 42-60%, the residues of the sophora flower buds after the extraction of the rutins of 28-40%, bran of 10-20%, gypsum of 0.6-2%, calcium superphosphate of 0.3-1%, carbamide of 0.2-1% and sugar of 0.8-2% and adding water into the cultivation raw materials after uniform mix of the cultivation raw materials to enable water content of the cultivation raw materials to achieve 55-65%; bagging and sterilizing after the burdening processing; inoculating and cultivating hypha; building a shed and earthing up; coloring; and managing fruiting. The mushroom cultivating method utilizing the residues of the sophora flower buds after the extraction of the rutins has the advantages of being simple in operation, capable of cultivating the mushroom by adding small amount of nutritional ingredients, quick in spawn runing, low in production cost and capable of effectively utilizing by-product residues in phytoextraction industries in a maximum limit and achieving sustainable development.

The invention discloses a method for cultivating wood-rotting fungi. The raw materials for cultivating the wood-rotting fungi comprise the following components of: 45-55 parts of cotton stalk crumb, 8-20 parts of fine sawdust, 8-20 parts of corncob, 5-20 parts of bran coat, 4-7 parts of bean dregs, 5-11 parts of pure chicken manure, 0.5-1.5 parts of phosphate fertilizer, 0.5-1.5 parts of plaster, 1-5 parts of lime and 8-20 parts of cotton seed hull. The raw materials in weigh ratio are cultivated to generate the wood-rotting fungi according to the steps of adding water in the raw materials and uniformly stirring, building pile and fermenting, producing an inoculation bacterial bag and generating mushroom. According to the method disclosed by the invention, the consumption of the cotton seed hull is reduced; and cotton straws are used to replace the cotton seed hulls and are simultaneously matched with the fine sawdust and the corncob to produce a cultivation raw material of the wood-rotting fungi. The method has the characteristics of ensuring quick material consumption by hypha during fingi fermentation, robust growth and white and density, compact produced fruiting body and rounding mushroom shape. According to the method, the labor and time are saved, the cost is reduced and the yield is increased.

The invention relates to the field of biology breeding and in particular relates to an agrobacterium-mediated ustilago esculenta (Ustilago esculenta) transformant strain as well as a preparation method and application thereof. According to the agrobacterium-mediated ustilago esculenta transformant strain, an agrobacterium infected receptor of the transformant strain refers to ustilago esculenta (Ustilago esculenta). The method for preparing the agrobacterium-mediated ustilago esculenta transformant strain comprises the following steps: (1) preparing a fungal expression vector; (2) preparing agrobacterium competence; (3) performing electrotransformation on the agrobacterium; and (4) performing agrobacterium-mediated transformation, thereby obtaining the transformant. A complex protoplast is not needed, the receptor source is simple, and spores and hypha of the fungi can be directly used for transformation; and the transformation efficiency is high. In addition, the obtained transformant is large in single-copy ratio, and marker genes are conveniently obtained.

The invention discloses two toadstool natural culturing methods belonging to one general invention conception. The first method comprises the following steps of mixing toadstool cultivated species with toadstool culturing materials and soil evenly, forming the mixture of the toadstool cultivated species, culturing materials and soil, sowing the mixture containing the toadstool cultivated species in a land and covering with soil with the thickness of 0.5cm-2.5cm; the toadstool culturing materials comprise herbaceous plant in grass family, crop straws, bran and KH2PO4, MgSO4, urea and other nutrient substances. The second method is as follows: hole sowing the toadstool cultivated species in the land directly and covering with soil with the thickness of 0.5cm-2.5cm. For the above two methods, at least one time of nutrition supplementation is carried out when mycelial is mature, and the nutrient supplementary solution is prepared by grass ash leachate, KH2PO4, MgSO4 and urea.

A method for culturing the Se-enriched edible fungus includes such steps as preparing culture medium from corn cob, straw powder, rice (or wheat) bran, gypsum, magnesium sulfate, zinc sulfate, Se salt and water, loading it in bogs, sealing, sterilizing, inoculating edible fungus spawn, fermenting, culturing mycelia, and spraying Se-enriched nutritive liquid. A method for producing Se-enriched milk by use of the leftover generated by culturing Se-enriched edible fungus features that the leftover is used as the additive of feed for quantitatively feeding the milk cow.

Disclosed is a lighting device for use in the control of a plant disease, which has a light source capable of emitting light containing ultraviolet ray. The light source can emit both of UV-B having a wavelength component with a wavelength of about 280 to 340 um and UV-C in which a wavelength component with a wavelength of about 255 nm or less is cut off from wavelength components with wavelengths of about 100 to 280 nm to plant in a superimposed manner. The irradiation of a plant with the UV-C and the UV-B ensures to further inhibit the spore formation or the hypha growth of a filamentous fungus that causes a disease or the like, and to induce a resistance against a disease in the plant.

The invention discloses a preparation method of a hypha / nanoparticle composite sphere material. The preparation method is characterized by comprising steps as follows: preparing a culture medium; adding 4-20 ml of a nanoparticle aqueous solution containing 1-10 mg of nanoparticles per milliliter to the sterilized liquid culture medium, evenly mixing the mixture, inoculating the mixture with strains, culturing the mixture for 48-96 h under conditions that the temperature is 15-35 DEG C and rotating oscillation is performed at the speed of 80-200 r / min to form a hypha / nanoparticle composite sphere material, performing filtration to remove a liquid, soaking solids, namely, hypha / nanoparticle composite spheres, with a sodium hydroxide aqueous solution for 12 h, washing the spheres with deionized water until the spheres are neutral, and then performing freeze-drying to prepare the hypha / nanoparticle composite sphere material. The composite sphere material prepared with the method is applicable to fields of industrial catalysis, wastewater treatment, biomedicine and the like and has the characteristics of low cost, high activity, easiness in recovery and the like.

The invention relates to an organic rich-selenium bio-fertilizer and a preparation method thereof. The organic rich-selenium bio-fertilizer comprises the following components in percentage by weight: 10-40% of livestock / poultry excrement, 20-50% of straw powder or humus soil, 20-40% of 80-mesh selenium ore powder containing 500 ppm of selenium, 0.02-0.07% of microbial soil repairing agent and 0.01-0.03% of zymocyte. The preparation method comprises the following steps: thoroughly and uniformly stirring the raw materials and stacking for fermentation; when the temperature reaches 40 DEG C, starting to turn the pile once every two days; when the temperature reaches 65 DEG C and hyphae appear, turning the pile; stacking for fermentation for three days; and crushing, screening, packing and storing the product in a storehouse. The method provided by the invention mainly thoroughly decomposes the raw materials containing various nutrients at a high temperature under the effect of microorganisms, converts inorganic selenium into an organic selenium fertilizer for the absorption by plants and animals, and produces rich-selenium organic products.

The invention relates to a method for cultivating microbial strains of phellinus linteus, which is characterized in that the culture media of microbial strains comprises the following components by weight percent: 75-85% of sawdust of poplar or willow or mulberry, 10-15% of bran, 5-8% of rice bran, 1-2% of plaster, 0.2-0.5% of monopotassium phosphate, 0.1-0.3% of magnesium sulfate, wherein, the sum of the weight percentage of the components is 100%. The microbial strains of phellinus linteus prepared in the invention has rapid growing of microbial strains of phellinus linteus, not easy ageing and yellow water occurred on surface hypha; and the strains can expand completely after 30-35 day cultivation. By adopting the method for cultivating a phellinus linteus forest land, forestry land resources can be fully utilized to plant phellinus linteus products; the method is simple and practicable; and the produced phellinus linteus with large volume can be artificially cultivated in great amounts, thus fully meeting the market demand.

The invention discloses an artificial cultivation method for Phellinus baumii Pilat. The method comprises the following steps: (1) inoculating the purified strain separated from a Phellinus baumii Pilat fruiting body to a mother seed culture medium so as to obtain a mother seed; (2) inoculating the mother seed to an original seed culture medium so as to obtain a Phellinus baumii Pilat original seed; (3) inoculating the Phellinus baumii Pilat original seed to a cultivar culture medium inside a cultivation bag for culture; and (4) transplanting the cultivation bag fully covered by hypha into a mushroom producing room and carrying out the mushroom producing culture under the following conditions: 85 to 95 percent of relative air humidity and 22 to 25 DEG C of temperature. The method finds out the best environmental factors such as the relative air humidity, the temperature, the illumination, and the like for the growth of the Phellinus baumii Pilat fruiting body. Through the artificial accurate control on the factors of relative humidity and temperature in the mushroom producing room, the method can maximally improve the growth speed of the Phellinus baumii Pilat fruiting body while simultaneously ensuring the quality of the Phellinus baumii Pilat fruiting body. The method has the advantages of high yield, high Phellinus baumii Pilat fruiting body quality, material conservation, short production cycle, and the like.

This invention relates to a manual culture method of selenium rich Cordyceps militaris and the application of fruit body. Firstly, the harvest wild bacterium of the Cordyceps militaris is purified normally, and the liquid strain which is produced by the purified strain is inoculated into the sterile incubation medium the content of selenium in which is rich. It is processed natural infection under the suitable situation and cultured continuously until the fruit body of Cordyceps militaris grows out. Otherwise the purified strain is produced into the liquid strain which is inoculated into the sterile incubation medium with frozen Cordyceps militaris powders. It is cultured continuously until the fruit body and hypha are filled full on the medium, spraying the selenium nutrient liquid until the Cordyceps militaris fruit body grows out. This technology decreases the regression of the strain and the decline of pharmacologic activity, and the operation is simple, the strain is stable, and the fruit body not only has the effective nutrient of Cordyceps militaris, but also it can hold the activity of the organic selenium long, the edible way is conveniently, and it is absolute no poison and the pharmacologic efficiency is notable.

The present invention relates to probiotic Propionibacterium strains and their use in the preparation of probiotic supplements and foods. The invention relates to the provision of Vitamin B12, propionic acid, folacin and bacteriocins by probiotic strains, stimulation of bifidobacteria growth, production of favourable effects on the lipid metabolism and on the immune system of hosts through immunostimulation, immunomodulation or use of a probiotic strain as an adjuvant, reduction of homocysteine and β glucuronidase and the prevention, treatment or amelioration of conditions associated with a need for these activities. The probiotic bacteria of the invention can be used in humans or other animals. In at least some applications, the bacteria can be used dead and parts rather than whole cells may be used. The present invention also relates to the preparation of vaccines for use in protecting patients from infectious diseases, in particular tuberculosis.

The invention discloses a method for culturing edible fungi, which includes the following steps: culturing the mother culture in larger scale to prepare liquid culture spawn, introducing the liquid culture spawn together with culture medium which needs to be sterilized separately to cultivating material for thick solid ventilated culturing, when the cultivating material is filled with hypha, briquetting and shaping the cultivating material, and sending the briquetted cultivating material to a mushroom house to produce mushroom. The method is environment-friendly, energy-saving, short in production period, low in pollution rate and small in work intensity.

The invention discloses a production method for planting agaricus bisporus by using mushroom residues of pleurotus eryngii. The agaricus bisporus consists of the following substances in percentage by weight: 40 to 60 percent of mushroom residues, 20 to 40 percent of corn straws, 10 to 30 percent of cow dung, 1 to 3 percent of urea, 1 to 3 percent of lime and 0.5 to 2.5 percent of calcium superphosphate. In the production method for planting agaricus bisporus by using the mushroom residues of pleurotus eryngii, the routine production process for culturing agaricus bisporus is adopted, wherein the process comprises the following steps of: pre-wetting raw materials, piling the materials, turning over the piles, placing and laying the piled materials on a frame, fermenting the materials, seeding the materials, mycelium culture, earthing, taking out and managing the agaricus bisporus and harvesting fruit bodies. Because the mushroom residues for producing pleurotus eryngii are recycled to serve as the material for planting agaricus bisporus, environmental pollution is avoided and the aim of clean growth and environmental protection is fulfilled.

The invention discloses a stropharia rugoso-annulata cultivation method. The method comprises the following steps of season arrangement, cultivation material selection, site selection, material collection, cultivation material treatment, sowing and mycelium cultivation, spawn running period management, earthing method and management after earthing, fruiting period management, harvesting, pest and disease control and processing. The method has the advantages that the stropharia rugoso-annulata cultivation method fully utilizes the straw resource, the specific steps and conditions of cultivation are particularly limited, and the yield of stropharia rugoso-annulata can be effectively increased; meanwhile, by adjusting the cultivation proportion of different kinds of straw, growth and development of the stropharia rugoso-annulata can be effectively controlled, the stropharia rugoso-annulata is high in yield, excellent in quality and capable of adapting to the market requirement.

The invention provides a method of producing worm grass lotus seeds by virtue of solid state fermentation of lotus seeds. The method comprises the following steps: on the basis of utilizing the steamed lotus seeds as a worm grass solid state fermentation culture medium, inoculating 3-8ml worm grass liquid strain to per 100 g of the lotus seeds; culturing for 10-30 days at the temperature of 5-30 DEG C and the humidity of 60-90% in dark, namely obtaining worm grass lotus seeds on which hyphae are overgrown; and culturing the worm grass lotus seeds on which the hyphae are overgrown at the temperature of 5-30 DEG C and the humidity of 80-95% for 10-30 days with the illumination of 8-15 hours per day, thus obtaining the worm grass lotus seeds on which worm grass sporocarps are grown. The invention provides a new worm grass source and a new lotus seed health food. According to the invention, the medical effect of worm grass and the nutrition of the lotus seeds are organically combined, so that the product has various nutrient components, is especially rich in active components such as cordycepin, a cordycepic acid, a worm grass polyose and the like and has high edible and medical values; and the worm grass lotus seed fungus preparation process is simple and can easily realize large-scale production, popularization and implementation.

The invention relates to a production method for a biomass packing material. The production method includes the steps that culture raw materials such as corn straw and wheat straw are evenly mixed and the sterilized at a high-temperature; a specific fungus strain is inoculated after cooling is conducted; then, the inoculated culture raw materials are loaded into a sterile packing mold and cultured for several days in a sterile environment; and then the culture raw materials are treated through drying and water removing under the normal-temperature conditions, and the biomass packing material is obtained. According to the production method, agricultural and sideline products such as the corn straw and the wheat straw are used as the raw materials, the characteristics that fungus hyphae grow rapidly, and hypha bodies are high in twisting force are utilized so that the crop straw can be converted into the biomass packing material, and the packing material has the beneficial effects of being environment-friendly, ecological and low in weight and cost; comprehensive recycling of the agricultural and sideline products is achieved, an existing foam packing material can also be replaced, good elasticity and buffering performance are achieved, the degrading performance is good, and environment pollution is relieved; and the biomass packing material is an ideal environment-friendly biomass packing material.

The invention discloses a method for culturing selenium-enriched Cordyceps militaris, which is to increase the selenium content in a culture medium to 50mg / L by using organic selenium yeasts instead of inorganic seleninic acid and sodium selenite, which infect the growth of hyphae, so as to ensure the selenium content in the cultured selenium-enriched Cordyceps militaris reaches 35 to 50mg / L. In the invention, the organic selenium yeasts which exert the lest inhibition effect on the growth of the Cordyceps militaris are adopted to increase the selenium content in the culture medium to 50mg / l, the selenium content in the cultured selenium-enriched Cordyceps militaris reaches 35 to 50mg / kg, which is 2 times higher than that of the Cordyceps militaris cultured in a culture medium added with inorganic selenium, and the yield of Cordyceps militaris is high.

The invention discloses a Grifola frondosa strain for producing polysaccharide with a composite raw material of rice bran and wheat bran, which belongs to the technical field of microbial application technology and food biology. The Grifola frondosa strain JSU10 is preserved in China general microbiological culture collection center (CGMCC) in October 8th, 2010 with the CGMCC No. 4179 and is identified to be Grifolasp. The invention improves the positive mutation rate of the Grifola frondosa strain for rapidly growing and producing Grifola frondosa polysaccharide on a composite culture medium of rice bran and wheat bran by composite mutagenesis of ultraviolet rays and microwave to obtain a high-yield strain; the strain and the original strain are respectively a liquid fermentation rice bran and wheat bran composite culture medium; and hypha dry weight and polysaccharide of the mutated strain are respectively improved by 39.24 percent and 42.58 percent compared with the original strain.

A method of creating an extract of myceliated agricultural product for human consumption includes providing an agricultural substrate such as rice, where the agricultural substrate has been inoculated by liquid media comprising an aliquot of culture derived from liquid-state fermentation. The culture being selected from the group consisting of Basidiomycota and Ascomycota fungi. Next, the step of enabling mycelium growth on the substrate by controlling temperature, humidity and sterility of the environment. After mycelium growth on the substrate reaches a desired stage, then the step of boiling the substrate in water and separating the water-substrate mixture into aqueous component and non-aqueous components creates an extraction.

The invention provides a cordyceps militaris fruiting body culturing method used yellow meal worm pupae as host. The fruiting body is cultured by using cordyceps militaris link to infect yellow meal worm. Its features are as follows: yellow meal worm is yellow brown or chocolate brown; fruiting body is bar type and orange yellow. The culturing method includes bacteria species preparing, yellow meal worm inoculating infecting, and out grass. The formed product has multi nutrient content and effect active constituent, and uses as foods. The culturing method is simple, effective, and reliable. And the cost is low.

The invention relates to a method for cultivating tricholoma lobayense by utilizing fungus dregs in the edible fungus production field, which is characterized by utilizing the leftovers, namely fungusdregs, of the edible fungi instead of cottonseed hulls to cultivate the tricholoma lobayense, utilizing the method of biological fermentation to treat the fungus dregs and adding bran and other nutrients to form culture media, and effectively realizing cultivation of the tricholoma lobayense through the process flows of bagging, sterilizing, inoculating, hypha fermenting, cultivating, bag removing, soil covering, fruiting and harvesting, etc., wherein, the leftovers of the edible fungi are used for cultivating oyster mushrooms and needle mushrooms, etc. The method not only realizes resource of the organic wastes and reduces pollution of the organic wastes on the environment by developing and utilizing the leftovers of the edible fungi and conforms to the requirement on striving to developcircular economy encouraged by the state, but also greatly reduces the usage amount of the cottonseed hulls and other substances for cultivation, effectively lowers the production cost of the tricholoma lobayense, increases the cultivation benefits and ensures the biological conversion ratio to be more than 80%. The method utilizes the wastes and attains two objectives, thus being suitable for popularization and application in the rural areas.

The present invention provides a formula of high-yield flammulina velutipes culture medium and a producing technique thereof. The invention relates to the technical filed of biological strain, and specially to the formula of high-yield flammulina velutipes culture medium and producing technique thereof. The corncob is used as main ingredient for replacing fir sawdust. The invention adopts 40-45% of corncob, 10-12% of bagasse, 25-30% of rice bran, 8-10% of bran, 4-6% of soybean hull and 2-3% of corn flour as the raw materials of flammulina velutipes culture medium. The method comprises the techniques of dry mixing, wet mixing, etc. The pH value is controlled between 7.0 and 7.5. The cultured flammulina velutipes has the advantages of strong activity of mycelium, high production volume, ordered mushroom generation, excellent commodity valve, ordered growing of sporphore, uniform magnitude of bonnet, white color, and no infection of foreign fungus. The unit production obtains 286-293grams / bottle. The dry object of each bottle is 260grams. The biological transformation efficiency reaches up to 110-113% and is increased for 30-40% compared with the common formula. The economic benefit is excellent.

The invention discloses a production method of grifola frondosa polysaccharide and relates to the technical field of food microorganism application. The method comprises the following steps of: weighing raw materials, wherein rice bran is 1-6g / 100mL, wheat bran is 1-3g / 100mL, monopotassium phosphate is 0.09-0.36g / 100mL, and magnesium sulfate is 0.04-0.16g / 100mL; adding a needed amount of water into the raw materials, loading the raw materials without regulating the pH (Potential of Hydrogen), and sterilizing and boiling at 121 DEG C for 30min; after cooling, inoculating the grifola frondosa liquid seed (CCTCC (China Center For Type Culture Collection) M2013286) which is also disclosed in the invention, wherein the inoculation amount is 8-10%, the fermentation temperature is 25-30 DEG C, and the fermentation time is 4-7d; and carrying out deproteinization, alcohol precipitation, centrifugal separation and vacuum drying on the centrifugal separation liquid of a fermented product or the centrifugal separation liquid which is obtained after the mycelia which has wall broken is extracted with hot water, so as to respectively obtain exopolysaccharide and mycelia polysaccharide. By adopting the method disclosed by the invention, the purpose of efficiently converting the rice bran and the wheat bran to the grifola frondosa polysaccharide with a high value through liquid state fermentation can be achieved better.

The invention discloses a bagged mushroom production method which comprises the steps of strain making, nutrient preparation, bagging, sterilization, inoculation, mycelium culture, coloring, inducement to primordium of mushroom and harvesting. The method is characterized in that 1, in the nutrient preparation step, 78% of miscellaneous tree sawdust, 20% of wheat bran, 1% of sucrose and 1% of gypsum in percentage by weight are evenly mixed, then 55-58% of water is added, and blending is performed; 2, the nutrient is put in barrel bags within 4 hours; 3, the bags are piled in the shape of a well and are sterilized; 4, when the bags are cooled to 25-28 DEG C, inoculation is performed; 5, the mushroom bags are transferred into a mushroom growth room of 20-25 DEG C and subjected to mycelium culture, and puncturing ventilation is performed twice in the mycelium culture period; and 6, in the mushroom growth management period, artificially-aided inducement to primordium of mushroom, dry stimulation and other treatment operations are performed. According to the invention, large-scale bagged mushroom production can be realized, the mushroom survival rate is high, the deformed mushroom defective rate is below 5%, the cost can be reduced and the product quality is high.

The invention belongs to the field of microbial application technology and food biotechnology, and discloses a ganoderma lucidum strain used for producing polysaccharides by complete feed liquid fermentation of rice bran and wheat bran. Ganoderma lucidum JSU6161314 is stored in China Center for Type Culture Collection (CCTCC) on 25th, June, 2013, preservation number is CCTCC M2013287, and the bacterial strain is named as Ganoderma lucidum CCTCC M2013287. According to a method disclosed in a drawing of the invention, the novel bacterial strain, which is capable of realizing high yield of ganoderma lucidum polysaccharides, is obtained by modifying ganoderma lucidum CFCC6043. The novel bacterial strain is used for rapid fermentation in a rice bran and wheat bran complete feed liquid culture medium and high yield of ganoderma lucidum polysaccharides. After fermentation, hypha dry weight and hypha polysaccharide yield increase 23.0% and 45% respectively compared with that of the original strain.

The invention discloses a large-scale cultivation method and a quality detection method of cordyceps militaris fruit bodies, comprising the steps of: (1) screening spawn; (2) preparing a rice solid culture medium (40-55 parts of rice and 45-60 parts of nutrient solution in every 100 parts by weight) and killing bacteria; (3) preparing liquid spawn: activating the spawn, inoculating the spawn into a liquid culture medium, conducting static culture for 24 hours and performing suspension culture on a shaking table; (4) culturing fruit bodies: inoculating the liquid spawn on the solid culture medium for dark culture for 6 days at the temperature of 20 DEG C until the surface of the medium is covered by mycelium; placing the well grown mycelium under incandescence with light intensity of 100 to 300 lux, wherein during a primordium growth phase, illumination is performed for 20-24 hours per day at the temperature of 20-24 DEG C; and during a fruit bodies growth phase, illumination is performed for 8-12 hours per day at the temperature of 18-20 DEG C and the fruit bodies are harvested 5 to 8 days after mature. The cordyceps militaris fruit bodies cultured by the method have the advantages of high yield and high content of active ingredients. Batches of cordyceps militaris fruit bodies have fingerprints with a similarity value greater than 0.950, indicating stable and controllable quality.

The invention provides a method for cultivating glossy ganoderma in a thick bamboo tube. In the cultivation of the glossy ganoderma, the thick bamboo tube is adopted to replace a fungi package; one end of the thick bamboo tube is open and the other end is reserved as the bottom; prepared culture medium is filled in the thick bamboo tube; after the opening is sealed, the sterilization and inoculation are performed according to convention; the thick bamboo tube is transferred into a culture room for hypha culture; after the hypha grows in the whole thick bamboo tube, the hypha is covered by earth to be cultivated in a field; the thick bamboo tube adopts annual bamboo or biennial bamboo, the bamboo is cut into the thick bamboo tube with one open end and one reserved bottom according to bamboo sections, and the thick bamboo tube is filled with the glossy ganoderma culture medium. The invention adopts the natural thick bamboo tube to replace polyethylene and polypropylene packages, and the thick bamboo tube has wide resource, can be repeatedly used, and is favorable for protection of ecological environment and recycling of resource; the cultivation process is simplified, prevents the pollution of fungi rods and plant diseases and insect pests, and improves the output and quality of the glossy ganoderma; and the glossy ganoderma also has the health care efficacy of the bamboo, and obvious economic benefit, and is suitable for promotion and application on a large scale.

A manufacturing method for cordyceps sinensis (Berk.) sacc polysaccharide relates to the technical field of food microorganism application, and comprises the following steps: weighing raw materials, wherein rice bran is 1-3 g per 100 ml, bran is 1-3 g per 100 ml, potassium dihydrogen phosphate is 0.09-0.18 g per 100 ml, and magnesium sulphate is 0.04-0.08 g per 100 ml; material filling with natural pH after adding required water, and sterilizing and cooking at the temperature of 121 DEG C for 30 min; inoculating cordyceps sinensis (Berk.) sacc CCTCCM2013285 liquid seed after cooling, wherein the inoculation amount is 8-10%, the fermentation temperature is 23-28 DEG C, and the fermentation time is 4-7 d; respectively obtaining exopolysaccharides and mycelia polysaccharide after conducting deproteinization, alcohol precipitation, centrifugal separation, and vacuum drying on centrifugal separation liquid extracted through breaking cell wall hot water of fermentation material centrifugal separation liquid and hypha. The method better realizes the purpose that rice bran and bran are efficiently and high-valuedly transformed to cordyceps sinensis (Berk.) sacc polysaccharide after being subjected to liquid state fermentation.