790 results about "Hypha" patented technology

The invention relates to the technical field of monitoring control, and provides an intelligent monitoring method for a dandelion and golden camellia fermentation process based on the Internet of Things, which comprises the following steps: deploying and self-calibrating a sensor array, extracting and analyzing various data features, and judging a fermentation stage and generating a regulation and control instruction according to the data features. According to the method, a precise fermentation monitoring and control method logic is formed, through omnibearing data acquisition and deep analysis, the change of each parameter in the fermentation process can be precisely grasped, the hypha growth rate, the flora metabolism intensity, the generation of a target product and other key indexes are monitored in real time, the fermentation conditions are adjusted in time, and the fermentation efficiency is improved. And the fermentation process is stabilized in an optimal state, so that the accuracy of fermentation production is greatly improved, the fluctuation of product quality is reduced, and the consistency and stability of the quality of each batch of dandelion and golden camellia are ensured.

The invention discloses bacillus velezensis GX0003742 and biocontrol application thereof, and belongs to the technical field of microorganism application. The invention discloses bacillus velezensis GX0003742 with a broad-spectrum bacteriostatic effect, which is preserved in the China General Microbiological Culture Collection Center, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is September 14, 2024, and the preservation number is CGMCC No.31981. The bacillus velezensis GX0003742 has the advantages that the bacillus velezensis GX0003742 can be used for preparing the bacillus velezensis GX0003742; the strain can obviously inhibit growth of colletotrichum basjoo mycelia and obviously reduce occurrence of postharvest diseases of bananas, has an inhibiting effect on various pathogenic bacteria, has a good insecticidal effect on auricular bean root-knot nematode, and provides a new strain resource for biological prevention and control of plant diseases.

The invention relates to the technical field of edible fungus cultivation and soilless culture, in particular to a preparation method and application of an organic ecological soilless culture substrate for dictyophora rubrovolvata. Auxiliary materials such as perlite, vermiculite, river sand and wood chips are mixed according to the proportion, and the soilless culture substrate is prepared. The matrix is completely used for soil-covered cultivation of dictyophora rubrovolvata, compared with a traditional cultivation mode, dictyophora rubrovolvata hyphae germinate quickly and grow vigorously, the conversion rate of mushroom buds (mushroom eggs) is high, the fruiting amount is large, meanwhile, the content of nutritional ingredients is increased, limited indexes such as heavy metal are obviously reduced, and the quality of dictyophora rubrovolvata products is effectively improved. According to the method, a new mode of soilless culture of edible mushrooms is constructed.

The invention belongs to the technical field of fungal gene engineering, and discloses a shiitake mushroom ABC transporter gene LeABC2 and application thereof in regulation and control of shiitake mushroom heat resistance. Whole genome association analysis is carried out based on heat resistance phenotype data of 133 shiitake mushroom strains, a candidate gene LeABC2 for regulating heat resistance is obtained, a LeABC2 gene RNA interference vector is constructed, the gene function is researched through an agrobacterium tumefaciens-mediated genetic transformation method, and the result shows that the LeABC2 gene negatively regulates the heat resistance of shiitake mushroom hyphae, the heat resistance of the hyphae can be improved by interfering the expression of the gene, and the gene has a good application prospect. A new solution thought is provided for breaking through the problem that the yield and quality are influenced by high temperature in the mushroom industry.

The invention belongs to the technical field of mushroom culture, and discloses a mushroom culture shelf lifting mechanism for a mushroom house, the mushroom culture shelf lifting mechanism comprises a rotating mechanism, the rotating mechanism comprises a first gear and a screw rod, bevel gears are fixedly connected to the sides, close to each other, of the first gear and the screw rod, and the screw rod is in threaded sleeve connection with a threaded sleeve; through cooperation of structures such as an inclined plate and a sliding rod, a rotating disc can conveniently rotate on a placement plate, after a first gear is meshed with a tooth groove, the rotating disc can drive a culture pot to rotate by 180 degrees and then continue to ascend and descend through transmission, it is ensured that each mushroom can receive light evenly, and the mushroom quality is improved. The illumination receiving time of different surfaces of mushrooms is kept consistent, so that the uniform growth of hyphae is promoted, the problem of excessive growth or uneven growth is avoided, meanwhile, different parts of a culture medium can obtain better conditions in the aspects of light receiving, atmospheric circulation and the like through rotation, uniform distribution and absorption of nutrients are promoted, and the growth rate of the hyphae is increased. The overall nutrition efficiency of mushroom growth is improved.

The invention discloses an intelligent nutrient circulation regulation and control method for a morchella esculenta fruit forest intercropping system based on the Internet of Things, particularly relates to the technical field of intelligent agriculture, and is used for solving the problem of symbiosis imbalance of hyphae and fruit trees caused by nutrient regulation and control lag and static threshold setting in the prior art. Hypha density, electric signal dominant frequency and root system absorption rate data are collected in real time through a multispectral impedance sensor and an ion selective electrode array, and a space-time aligned dynamic matrix is constructed; dynamically updating a nutrient balance threshold value based on a real-time ratio of a hypha metabolism rate to an absorption rate, and generating a nutrient transfer priority by combining collaborative analysis of a hypha transfer direction included angle and an electric signal main frequency offset gradient; a continuous transfer path is identified through a region growing algorithm, water and fertilizer amounts are distributed according to priorities, and a local area instruction is generated; and inverting the equipment response delay by using the high-frequency pulse signal, synchronously adjusting the execution frequency and updating the dynamic matrix and the threshold parameter, thereby realizing the dynamic adaptation and autonomous optimization of the nutrient supply and demand of the intercropping system.

The invention relates to application of turmeric rhizome extract emulsifiable concentrate in preventing and treating corn leaf spot and tomato gray mold. Compared with the prior art, the turmeric rhizome extract missible oil disclosed by the invention contains an effective substance aromatic turmerone, and the aromatic turmerone can reduce the spore germination rate and sporulation quantity of pathogenic bacteria, increase the permeability of hypha cell membranes and cause overflow of contents, so that normal growth of hyphae is inhibited, and an antibacterial effect is achieved. Aromatic turmerone acts on plants, so that plant cells generate more polyphenol and flavonoid substances, and the immunity of the plants is improved. Pharmacodynamic test results in fields in two fields show that the 6.5% turmeric rhizome extract emulsifiable concentrate can be used for stably and effectively preventing and treating corn northern leaf blight and tomato gray mold after being diluted by 150-300 times, and also has a yield increasing effect.

The invention discloses a strain of arthrosporium spp. And application thereof in prevention and treatment of pine wood line disease. The invention relates to Arthrobotrys heuanensis 18GD1705A, which is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 15, 2021, the address of the Arthrobotrys heuanensis is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the postal code is 100101, and the preservation number is CGMCC No.23851. The Arthrobotrys heuanensis 18GD1705A has the advantages that the Arthrobotrys heuanensis 18GD1705A can be used for preparing a microbial inoculant; according to the method disclosed by the invention, the river-source arthrosporium is inoculated into pine seedling bodies, and the nematodes are caught by utilizing a three-dimensional viscous bacterium net formed by special hypha metamorphosis and can also be killed by forming a structure similar to a contraction ring through singular contraction. Meanwhile, before the pine wood nematodes colonize the pine trees, the river source arthrosporium inoculant is inoculated into the pine trees, so that the tree vigor of the pine seedlings can be effectively enhanced, and a better prevention effect is achieved.

The invention provides a bacillus velezensis strain and application thereof. The name of the bacillus velezensis is BJ-1141, and the bacillus velezensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32094. The strain has broad-spectrum antibacterial activity, and has a good inhibition effect on 24 plant pathogenic fungi such as rice pathogenic bacteria, wheat pathogenic bacteria, corn pathogenic bacteria, grape pathogenic bacteria, cherry pathogenic bacteria, solanaceous crop pathogenic bacteria and the like. Wherein the growth inhibition effect on sclerotinia sclerotiorum hyphae causing eggplant sclerotinia rot is optimal, and the inhibition rate can reach 95.57%. The BJ-1141 bacterial liquid has an obvious growth promoting effect on the growth of plants such as tomatoes and lettuce. After the BJ-1141 bacterial liquid is used for irrigating roots of potted tomatoes, the tomato fusarium wilt caused by tomato fusarium oxysporum can be effectively controlled, the BJ-1141 bacterial liquid has a good prevention and treatment effect, the prevention and treatment effect can reach 85.93%, and the prevention and treatment effect is obviously different from that of a control group.

The invention provides a Trichoderma asperellum TAZ19, a biocontrol inoculant and application of the Trichoderma asperellum TAZ19, the Trichoderma asperellum TAZ19 is preserved in the China Center for Type Culture Collection on October 25, 2024, and the preservation number of the Trichoderma asperellum TAZ19 is CCTCC M 20242341. The active ingredients of the biocontrol inoculant comprise the trichoderma asperellum TAZ19 strain, and the trichoderma asperellum TAZ19 strain is one or more of conidia, hypha, fermentation filtrate or active extracts of the trichoderma asperellum TAZ19 strain of the trichoderma asperellum TAZ19 strain. The trichoderma asperellum TAZ19 has an excellent antagonistic effect on a plurality of plant disease pathogenic bacteria such as dispora pinnatifida, a plurality of fusarium oxysporum, apple black beancurd skin fungi and botryosphaeria dothidea, has broad-spectrum antibacterial activity, is high in growth speed, high in antibacterial activity and high in hyperparasitism ability, has better saline-alkaline tolerance, also has a growth promoting effect on plants, and can be widely applied to the field of plant diseases. The strain can be used as an active component of a biocontrol inoculant to be widely applied to prevention and treatment of various plant diseases such as pine tree branch rot, wheat stem rot and tomato fusarium wilt, and has a wide application prospect.

The invention discloses a mother strain culture medium for boletus xylophilus as well as a preparation method and application of the mother strain culture medium, and belongs to the technical field of microbial culture. The preservation number of the strain JSJ-Bx1 of the boletus sinensis mother strain is CGMCC (China General Microbiological Culture Collection Center) No.42007, and the strain JSJ-Bx1 is preserved in the China General Microbiological Culture Collection Center; the culture medium is prepared from 10-30 g of a carbon source, 2-6 g of a nitrogen source, 1-3 g of inorganic salt, 0.1-0.3 g of a growth factor suitable for the culture medium, 20 g of agar and 1 L of water, and the pH of the culture medium is 4-6. By the adoption of the culture medium, hyphae of the boletus sinensis mother strain grow fast, grow vigorously and are dense, the edges of bacterial colonies are neat, hypha degeneration phenomena such as pigment and water spitting are avoided, and the mother strain culture growth cycle is greatly shortened.

The present invention relates to a method for preparing fungal biomass comprising the step of cultivating at least one fungal strain according to conditions of fungal biomass cultivation, wherein said fungal biomass is characterized by a target property, wherein the method further comprises predicting how to cultivate said fungal biomass to achieve said target property, and to relating prediction method. The present invention further relates to a method for preparing fungal biomass comprising the step of cultivating at least one fungal strain according to conditions of fungal biomass cultivation, wherein said fungal biomass is characterized by a target property, wherein the method further comprises predicting a target property of said fungal biomass based on the conditions of fungal biomass cultivation, and to relating prediction method. The present invention further relates to biomass obtainable according to the methods of the present invention.

The invention discloses a multi-factor coupling ganoderma lucidum environment digital twinborn regulation and control method oriented to high-yield requirements, particularly relates to the technical field of intelligent cultivation of edible mushrooms, and is used for solving the problems of equipment action oscillation and recessive physiological stress caused by physiological response hysteresis of ganoderma lucidum in an existing environment regulation and control system. Carbon dioxide concentration and illumination intensity predicted values are generated in real time by constructing ganoderma lucidum growth digital twins, and an oxidative stress mark is activated by combining hypha respiratory entropy matching degree analysis and an active oxygen accumulation curve; when the matching degree exceeds a threshold value and oxidative stress exists, a metabolic overload signal is generated, and whether the carbon dioxide predicted value is in a physiological hysteresis interval or not is judged; delay compensation and metabolic overload suppression coefficient adjustment are performed on the carbon dioxide predicted value in the delay interval; and finally, inputting the compensated carbon dioxide value and the illumination prediction value into an environment factor coordination rule base, generating a ventilation opening degree and light supplementing power instruction to drive execution equipment, and ensuring the morphological development stability of the sporocarp and the synthesis efficiency of the active component.

The invention relates to the technical field of agricultural micro-ecological application, and particularly discloses a composite combined inoculation method using arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria, which comprises the following steps: selecting corn plant seeds without plant diseases and insect pests; a corn planting land is randomly divided into land parcels with the area of 6 m < 2 > (2 m * 3 m), a buffer zone of 1.0 m is arranged between every two adjacent land parcels, and the cultivation mode is flat cropping; the method comprises the following steps: planting arbuscular mycorrhizal fungi inoculum comprising mycorrhiza, dry sand containing spores and ectotrophic hyphae and corn seeds in soil by using a seeder during corn sowing, and after the corn is planted for 30 days, inoculating the plant growth-promoting rhizobacteria inoculum into plants and soil by using a flood irrigation root irrigation method, when the arbuscular mycorrhizal fungi and the plant growth-promoting rhizobacteria are inoculated together, the corn rhizosphere microbial community can be changed, the richness and diversity of fungi and bacterial communities in soil are increased, and the relative abundance of plant pathogens in the soil is reduced.

The invention belongs to the technical field of edible fungus cultivation, and discloses an automatic bag removing device for edible fungus matrixes, which comprises a processing table, a six-edge support is fixedly mounted on the processing table, lifting cylinders are fixed on the upper and lower end faces of the six-edge support, and cutting knife rests are fixed at the output ends of the lifting cylinders. Six groups of tearing structures for clamping and expanding the bag body are mounted on the six-edge bracket at equal angles; electric sliding rails are symmetrically fixed to the two sides of the upper surface of the machining table, a sliding support is slidably installed on the electric sliding rails, an outer positioning ring is fixed to the sliding support, a rotating frame is rotatably installed in the outer positioning ring, and a limiting structure for clamping and limiting a bag body filled with edible fungus matrixes is arranged on the rotating frame. The bag body can be clamped, expanded and cut apart, so that the bag body is effectively torn and separated from a mushroom stick, damage to hyphae in the mushroom stick is avoided in the separation process, and the bag removing effect is good.

The invention is suitable for the technical field of ganoderma lucidum cultivation, and provides a ganoderma tsugae and leukoderma lucidum cultivation and planting device and method.The ganoderma tsugae and leukoderma lucidum cultivation and planting device comprises a cultivation box, and an isolation frame is fixedly installed in the cultivation box so that the interior of the cultivation box can be isolated into a plurality of independent cultivation cavities; a plurality of pick-and-place openings are formed in one side of the cultivation box and correspond to the multiple cultivation cavities respectively, cultivation drawers are arranged in the multiple cultivation cavities in a drawing mode and used for containing culture media to cultivate hyphae, and the cultivation drawers can pass through the pick-and-place openings. According to the ganoderma tsugae and leukoderma lucidum cultivation and planting device and method, the cultivation box is divided into the multiple cultivation cavities through the isolation frames, each cultivation cavity is provided with an independent taking and placing opening, physical isolation of a hypha cultivation environment is achieved, cross contamination is avoided, the hoisting plate is fixed to the lower portion of the sliding piece, and a camera and a sterilizing lamp are carried. And the hyphae in the cultivation drawer can be monitored in real time and dynamically disinfected.

The invention relates to a spaceflight-carried schizophragma integrifolium strain Kungsu No.3 and application thereof, and belongs to the technical field of strain breeding. The spaceflight-carried schizophyllum commune strain No.3 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 9, 2024, the preservation number is CGMCC No.41428, the classification name of the spaceflight-carried schizophyllum commune strain is schizophyllum commune, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Compared with cultivation of strains which are not subjected to spaceflight carrying, the Kunzhou No.3 strain is fast in hypha germination, fast in growth, crisp in sporocarp, free of or few in spores, light pink to pink, high in conversion rate, short in period and suitable for industrial cultivation.

The invention provides a coal gangue-based soil matrix as well as a preparation method and application thereof, and belongs to the technical field of ecological restoration. According to the invention, the coal gangue with continuous gradation is adopted, so that the coal gangue-based soil matrix has the particle size distribution of natural soil; urea is added to adjust the carbon-nitrogen ratio of the coal gangue and the biomass waste, and a microbial agent is added for pre-curing, so that the microbial activity is improved, complete decomposition of organic matters is promoted, and formation of water-stable aggregates is promoted; multi-layer root plants are planted together and inoculated with fungi, formation of water-stable aggregates is promoted through root interpenetration and a hypha network, meanwhile, the nutrient circulation efficiency is improved together with root exudates and microbial metabolism, and nutrient release is more balanced. Through multi-dimensional symbiotic regulation and control, the coal gangue-based soil matrix has particle size distribution of natural soil, nutrient balance, high water-stable aggregate content and organic matter content, and is more beneficial to ecological restoration.

The invention discloses a method for enhancing Candida albicans biofilm resistance of azole drugs by using a zinc ion chelating agent, through the combined action of the zinc ion chelating agent N, N, N ', N'-tetra (2-picolyl) ethylenediamine (TPEN) and fluconazole, the activity of the Candida albicans biofilm can be reduced, and the Candida albicans biofilm can be enhanced. The early-stage adhesion process, the medium-stage yeast-to-hypha conversion process and the late-stage maturation process formed by the candida albicans biological membrane are inhibited, the structure of the candida albicans biological membrane is destroyed, then the high drug resistance of the candida albicans biological membrane to fluconazole is reversed, and the pathogenicity of the fluconazole is reduced; the problems of high drug resistance and sustained infection caused by the formation of a candida albicans biofilm are hopefully solved, and a direction is provided for the treatment of clinical fungal infection and the research and development of drugs.

The invention relates to the technical field of biocontrol strains for crops, in particular to bacillus velezensis JR1A and application thereof to inhibition of garlic pathogenic fungi. The bacillus velezensis JR1A is preserved in the China General Microbiological Culture Collection Center on June 30, 2025, and the preservation number of the bacillus velezensis JR1A is CGMCC No.35059. The bacillus velezensis JR1A is named as bacillus velezensis JR1A. The JR1A inhibits mycelial growth and spore production of pathogenic fungi of common garlic diseases, has an excellent biocontrol effect and a certain growth promoting function, can reduce the relative abundance of pathogenic fungi in garlic crop rotation soil, and reduces the occurrence of related garlic diseases.

The invention discloses an artificial cultivation method of xylaria fungus sclerotium, and relates to the field of sclerotium cultivation. The method comprises the following steps: collecting xylaria producing sclerotia, obtaining spores by adopting a spore separation method or obtaining core tissues by adopting a tissue separation method, inoculating the spores or the core tissues on an enriched PDA culture medium to be cultured into bacterial colonies, selecting hyphae with vigorous growth vigor at the edges of the bacterial colonies, and performing purification culture until a mother strain only containing a single strain is obtained; performing gene sequencing to identify the strain; propagating the mother strain to obtain an original strain, inoculating the original strain into a sterilized solid culture medium, culturing in a dark place to obtain a cultivated strain, placing the cultivated strain on soil, reserving a space for sclerotium development around the cultivated strain, culturing in the dark place for 33-90 days, and harvesting the sclerotium. The xylaria sclerotium with single germplasm can be cultivated on a large scale, and the problems of wild resource shortage and germplasm mixing are effectively solved.

The invention belongs to the technical field of water treatment, and particularly provides a nitrogen-oxygen co-doped hypha / spruce composite adsorption material and a copper-containing sewage treatment method. According to the invention, the copper-containing sewage is pretreated by using the solid fermentation product, and then the copper-containing sewage is post-treated by using the nitrogen-oxygen co-doped hypha / spruce composite adsorption material prepared under specific conditions; wherein in the pretreatment stage, microbial metabolites in a solid fermentation product preliminarily capture free copper ions (Cu < 2 + >) through complexation, the toxicity of the sewage is reduced, and meanwhile, the copper ions are partially converted into a form which is easier to adsorb. In the post-treatment stage, the nitrogen-oxygen co-doped hypha / spruce composite adsorption material plays a key role, and efficient interception and adsorption of copper ions are achieved. In conclusion, the treatment method for the copper-containing sewage comprises a pretreatment stage and a post-treatment stage, the two stages are in synergistic interaction, and finally the removal rate of the heavy metal copper can be effectively increased.

The invention discloses a cordyceps sinensis intelligent bionic cultivation control system and method based on the Internet of Things, and the method comprises the steps: collecting the temperature and humidity, gas concentration, illumination intensity and larva behavior data of a cordyceps sinensis matrix through a sensor network, and carrying out the fusion through an edge calculation gateway, and generating a dynamic environment map; constructing a plateau ecological digital twinborn model based on the dynamic environment map in combination with historical meteorological data and a host-strain interaction rule, and generating a staged control time sequence; based on a staged control time sequence, temperature and humidity coupling compensation is carried out through a feedforward-feedback composite algorithm, and actuator linkage is adjusted through fuzzy PID; aI vision is used for identifying a larva infection window period and accurate spraying is carried out, and hypha infection is monitored in combination with a near infrared spectrum to dynamically adjust the CO2 concentration; and constructing an LSTM (Long Short Term Memory) model to predict the growth risk in combination with a dynamic environment map and insect body monitoring data, and actively intervening in an abnormal environment. According to the invention, high-precision, dynamic and intelligent regulation and control of the growth environment of cordyceps sinensis are realized.

The invention discloses a method for cultivating coprinus comatus by using white spirit vinasse, and belongs to the technical field of edible mushroom production. In order to realize production of coprinus comatus by using vinasse instead of cottonseed hulls, the invention provides a method for cultivating coprinus comatus by using liquor vinasse, which comprises the following steps: uniformly mixing oyster mushroom bran, vinasse with the pH value of 9-10 and bran, and adjusting the moisture to 55-65% to obtain a cultivation material; after sterilization, coprinus comatus strains are inoculated, and the coprinus comatus strains are cultured under the conditions that the temperature is 25-31 DEG C, the relative air humidity is 65-75% and no light exists until hyphae grow fully; then adding a soil covering material, and culturing for 8-10 days; and irradiating with scattered light at the temperature of 16-22 DEG C and the relative air humidity of 85-90%, culturing for 7-13 days, and picking. By optimizing the cultivation material formula and the mycelium culture temperature, the growth cycle of the coprinus comatus is shortened, the fruiting rate of the coprinus comatus is increased, the mushroom quality is improved, the production cost is reduced, and a new way is provided for edible mushroom production.

The invention belongs to the technical field of genetic engineering of edible mushrooms, and particularly relates to a construction method of an efficient and stable genetic transformation system of flammulina velutipes. The construction method comprises the following steps: culturing in an MM culture medium by taking a flammulina velutipes strain 0747 as a receptor; the pFgnpt vector is converted into agrobacterium AGL1 (AGL1); inoculating hyphae into an IM liquid culture medium containing acetosyringone and MES, mixing with agrobacterium tumefaciens, culturing, and transferring the hyphae to an IM solid flat plate paved with filter paper; hyphae are transferred to a PDA resistance plate containing G418 and cephalosporin for resistance screening, and an efficient and stable flammulina velutipes genetic transformation system is obtained. The new selection marker G418 is developed and applied, limitation of a traditional selection marker is broken, more choices are provided for flammulina velutipes genetic transformation, a new thought is provided for genetic transformation research of other edible mushrooms, and the application field of an agrobacterium tumefaciens-mediated transformation technology is expanded.

The invention provides inter-plant mycorrhiza nutrient transfer culture equipment which comprises a culture box body and an organic glass plate, an inner cavity of the culture box body is divided into a first culture chamber and a second culture chamber through the organic glass plate, the organic glass plate is a porous glass plate, and a white nylon net is pasted on one side of the porous glass plate. And the white nylon net is divided into a white nylon net with the aperture of 0.45 mu m and a white nylon net with the aperture of 37 mu m. The equipment is small in size, small in occupied area, cheap in material, convenient to assemble and suitable for pot culture experiments of large-batch samples, soil ions are allowed to pass through by means of 0.45 microns, ectogenic hyphae and the soil ions are allowed to pass through by means of a white nylon net of 37 microns, the soil ions, the ectogenic hyphae and root systems are allowed to pass through a nylon-free net, and the soil ions, the ectogenic hyphae and the root systems are allowed to pass through. Compared with the prior art, direct and indirect paths of nutrient transfer between plants can be distinguished and quantified, and the method is simple and easy to implement.

The invention relates to the technical field of image recognition, in particular to a horticultural product fungal disease image recognition system and method, and the system comprises an image collection module, an image segmentation module, an anomaly detection module, a feature extraction module, a disease recognition module and a decision output module. In the prior art, the intensity of a light supplementing lamp is manually adjusted, and instantly changing illumination conditions (such as cloud layer shielding and tree shadow shaking) are difficult to deal with in an open-air plantation, so that leaf scab details are often submerged by strong light or dim light is blurred; according to the scheme, a closed-loop linkage system of ambient light perception and an annular LED is innovatively deployed, and the light supplementing intensity is reversely adjusted in a millisecond level by tracking ambient brightness in real time, so that purple gray hyphae of downy mildew in mist in the morning and anthracnose black spots under the noon burning sun present completely consistent color saturation and texture definition in imaging; and the problem of misdiagnosis caused by illumination fluctuation in the traditional method is thoroughly solved.

The invention discloses a hypha network reinforced ecological slope protection structure and a construction method.The hypha network reinforced ecological slope protection structure comprises a plurality of ecological soil nails arranged on a side slope, and each ecological soil nail comprises a soil nail head located outside the side slope; a hollow shell extending into the slope is arranged at one end of the soil nail head, a grouting hole communicated with the hollow shell is formed in the soil nail head, and a plurality of leakage holes are formed in the surface of the hollow shell; a plurality of ecological panels are arranged on the inclined face of the side slope, and the ecological soil nails are filled with grouting liquid with hypha spores. An assembly type structure frame is formed by inserting and matching the ecological panels and the ecological soil nails, grouting liquid with spore bags is injected, after the spore bags are activated, mycelium is combined in a winding mode in the growth process, and a high-ductility biological fiber network structure is constructed between soil particles and components. And the structural integrity and the shear resistance and disturbance resistance of the side slope are improved.

The invention provides a fluorescence image recognition system for detecting fungi based on a deep learning algorithm. The fluorescence image recognition system comprises an image acquisition module, a single spore and hypha recognition module and a hypha group recognition module, the image acquisition module is used for acquiring a target fluorescence image; and the single spore and hypha identification module and the hypha mass identification module are used for inputting the target fluorescence image into a pre-established deep learning algorithm model, identifying the single spore or hypha and the hypha mass through the pre-established deep learning algorithm model, and outputting a fungus negative or positive result according to an identification result. Compared with the prior art, after the target fluorescence image is obtained by the image acquisition module, the target fluorescence image is input into the pre-established deep learning algorithm model for automatic analysis, and analysis of spores / hyphae and hypha masses which are greatly different in form is divided into two modules for processing; and spores, hyphae and hypha masses can be identified and distinguished more accurately.

The invention relates to a cavern environment-based tricholoma matsutake artificial cultivation method, and relates to the technical field of edible fungus cultivation, and the method comprises the following steps: mushroom stick preparation: mixing pine sawdust, corncob, bran, rice bran and an additive, adding water, bagging, sterilizing with high-pressure steam or normal-pressure steam, and inoculating tricholoma matsutake strains into each bag; environment construction: installing temperature and humidity regulation and light scattering equipment in cave depots, natural caves and air-raid shelters with the soil layer thickness of more than 50 m, the constant temperature of 10-20 DEG C and the humidity of 70-90%; in the hypha stage, the temperature is controlled to be 15-18 DEG C, the humidity is controlled to be 70-80%, and culture is conducted for 40-60 days; in the fruiting stage, the temperature is lowered to 12-16 DEG C in a stepped mode, the humidity is increased to 85-90%, and harvesting is conducted after fruiting bodies are induced to be formed. And recycling resources: crushing the waste mushroom sticks, and mixing the crushed waste mushroom sticks with soil according to a ratio of 1: 3 to prepare a fertilizer or mixing 10-30% of a new material for reuse. The produced tricholoma matsutake has the tricholoma matsutake alcohol content of 77.21 mg / kg and the polysaccharide content of 2.67%, the active components of the tricholoma matsutake are equal to those of wild tricholoma matsutake, the hypha germination rate is greater than or equal to 95%, the growth period is shortened to 40-60 days, and the raw material consumption is reduced by