395 results about "Hypha" patented technology

The invention discloses a multi-factor coupling ganoderma lucidum environment digital twinborn regulation and control method oriented to high-yield requirements, particularly relates to the technical field of intelligent cultivation of edible mushrooms, and is used for solving the problems of equipment action oscillation and recessive physiological stress caused by physiological response hysteresis of ganoderma lucidum in an existing environment regulation and control system. Carbon dioxide concentration and illumination intensity predicted values are generated in real time by constructing ganoderma lucidum growth digital twins, and an oxidative stress mark is activated by combining hypha respiratory entropy matching degree analysis and an active oxygen accumulation curve; when the matching degree exceeds a threshold value and oxidative stress exists, a metabolic overload signal is generated, and whether the carbon dioxide predicted value is in a physiological hysteresis interval or not is judged; delay compensation and metabolic overload suppression coefficient adjustment are performed on the carbon dioxide predicted value in the delay interval; and finally, inputting the compensated carbon dioxide value and the illumination prediction value into an environment factor coordination rule base, generating a ventilation opening degree and light supplementing power instruction to drive execution equipment, and ensuring the morphological development stability of the sporocarp and the synthesis efficiency of the active component.

The invention discloses an artificial cultivation method of xylaria fungus sclerotium, and relates to the field of sclerotium cultivation. The method comprises the following steps: collecting xylaria producing sclerotia, obtaining spores by adopting a spore separation method or obtaining core tissues by adopting a tissue separation method, inoculating the spores or the core tissues on an enriched PDA culture medium to be cultured into bacterial colonies, selecting hyphae with vigorous growth vigor at the edges of the bacterial colonies, and performing purification culture until a mother strain only containing a single strain is obtained; performing gene sequencing to identify the strain; propagating the mother strain to obtain an original strain, inoculating the original strain into a sterilized solid culture medium, culturing in a dark place to obtain a cultivated strain, placing the cultivated strain on soil, reserving a space for sclerotium development around the cultivated strain, culturing in the dark place for 33-90 days, and harvesting the sclerotium. The xylaria sclerotium with single germplasm can be cultivated on a large scale, and the problems of wild resource shortage and germplasm mixing are effectively solved.

The invention belongs to the technical field of water treatment, and particularly provides a nitrogen-oxygen co-doped hypha / spruce composite adsorption material and a copper-containing sewage treatment method. According to the invention, the copper-containing sewage is pretreated by using the solid fermentation product, and then the copper-containing sewage is post-treated by using the nitrogen-oxygen co-doped hypha / spruce composite adsorption material prepared under specific conditions; wherein in the pretreatment stage, microbial metabolites in a solid fermentation product preliminarily capture free copper ions (Cu < 2 + >) through complexation, the toxicity of the sewage is reduced, and meanwhile, the copper ions are partially converted into a form which is easier to adsorb. In the post-treatment stage, the nitrogen-oxygen co-doped hypha / spruce composite adsorption material plays a key role, and efficient interception and adsorption of copper ions are achieved. In conclusion, the treatment method for the copper-containing sewage comprises a pretreatment stage and a post-treatment stage, the two stages are in synergistic interaction, and finally the removal rate of the heavy metal copper can be effectively increased.

The invention belongs to the technical field of genetic engineering of edible mushrooms, and particularly relates to a construction method of an efficient and stable genetic transformation system of flammulina velutipes. The construction method comprises the following steps: culturing in an MM culture medium by taking a flammulina velutipes strain 0747 as a receptor; the pFgnpt vector is converted into agrobacterium AGL1 (AGL1); inoculating hyphae into an IM liquid culture medium containing acetosyringone and MES, mixing with agrobacterium tumefaciens, culturing, and transferring the hyphae to an IM solid flat plate paved with filter paper; hyphae are transferred to a PDA resistance plate containing G418 and cephalosporin for resistance screening, and an efficient and stable flammulina velutipes genetic transformation system is obtained. The new selection marker G418 is developed and applied, limitation of a traditional selection marker is broken, more choices are provided for flammulina velutipes genetic transformation, a new thought is provided for genetic transformation research of other edible mushrooms, and the application field of an agrobacterium tumefaciens-mediated transformation technology is expanded.

The invention relates to the technical field of image recognition, in particular to a horticultural product fungal disease image recognition system and method, and the system comprises an image collection module, an image segmentation module, an anomaly detection module, a feature extraction module, a disease recognition module and a decision output module. In the prior art, the intensity of a light supplementing lamp is manually adjusted, and instantly changing illumination conditions (such as cloud layer shielding and tree shadow shaking) are difficult to deal with in an open-air plantation, so that leaf scab details are often submerged by strong light or dim light is blurred; according to the scheme, a closed-loop linkage system of ambient light perception and an annular LED is innovatively deployed, and the light supplementing intensity is reversely adjusted in a millisecond level by tracking ambient brightness in real time, so that purple gray hyphae of downy mildew in mist in the morning and anthracnose black spots under the noon burning sun present completely consistent color saturation and texture definition in imaging; and the problem of misdiagnosis caused by illumination fluctuation in the traditional method is thoroughly solved.

The invention provides a fluorescence image recognition system for detecting fungi based on a deep learning algorithm. The fluorescence image recognition system comprises an image acquisition module, a single spore and hypha recognition module and a hypha group recognition module, the image acquisition module is used for acquiring a target fluorescence image; and the single spore and hypha identification module and the hypha mass identification module are used for inputting the target fluorescence image into a pre-established deep learning algorithm model, identifying the single spore or hypha and the hypha mass through the pre-established deep learning algorithm model, and outputting a fungus negative or positive result according to an identification result. Compared with the prior art, after the target fluorescence image is obtained by the image acquisition module, the target fluorescence image is input into the pre-established deep learning algorithm model for automatic analysis, and analysis of spores / hyphae and hypha masses which are greatly different in form is divided into two modules for processing; and spores, hyphae and hypha masses can be identified and distinguished more accurately.

The invention discloses a hypha mineral composite bionic sand stabilization unit body and a preparation method thereof, and relates to the technical field of sand stabilization, the interior of the unit body is provided with a bionic three-dimensional porous skeleton structure, the unit body comprises a porous core layer, a porous core layer and a porous core layer, the porous core layer takes cleaning sand grains with the particle size of 0.1-2 mm as a sand-based carrier; uniformly mixing and curing with organic nutrient substances, mineral precursors and strain spores; the composite reinforcing layer is formed by interweaving and compounding a mycelium network formed by germination and growth of strain spores, mineral crystals generated by in-situ mineralization induced by metabolic activity of hyphae and a plant fiber reinforcing material which is externally added and embedded into the pores in the pores of the porous core layer; according to the hypha mineral compounded bionic sand stabilization unit body and the preparation method thereof, mold pressing or 3D printing prefabrication is adopted, the structure is controllable, and standardized production and transportation are facilitated; and through a controllable fermentation and gradient dehumidification mineralization culture process, hyphae and minerals are ensured to be uniformly and fully compounded in pores, and the product quality is stable.

This invention discloses a Chinese saprophytic bolete ( Buchwaldoboletus xylophilus This invention relates to the mother culture medium, its preparation method, and its application, belonging to the field of microbial culture technology. The strain JSJ-Bx1 of the *Boletus spp.* mother culture, with accession number CGMCC No. 42007, is deposited at the China General Microbiological Culture Collection Center. The culture medium composition is: 10–30 g carbon source, 2–6 g nitrogen source, 1–3 g inorganic salts, 0.1–0.3 g suitable growth factors, 20 g agar, and 1 L water, with a pH of 4–6. Using the culture medium of this invention, the mycelium of the *Boletus spp.* mother culture exhibits rapid, vigorous, and dense growth, with neat colony edges, and does not produce pigments or exhibit mycelial degeneration such as exudation, significantly shortening the mother culture growth cycle.

The invention belongs to the technical field of temperature control, and particularly relates to an intelligent temperature control method for an edible mushroom cultivation growth room, which comprises the following steps of: judging temperature abnormity by combining a comprehensive evaluation function of an instantaneous overrun proportion and a standard deviation, and determining a regulation priority according to the temperature abnormity, so that the conversion from average temperature control to abnormal region priority regulation is realized; a shelf two-dimensional temperature distribution diagram is constructed, gradient calculation is carried out, a high-temperature area and the orientation thereof are accurately positioned, the deflection direction and angle of a guide plate are directly controlled through coordinate transformation and nonlinear mapping, cold air flow preferentially flows to the high-temperature area, the problem of uneven horizontal and vertical temperature caused by a three-dimensional cultivation shelf is effectively solved, and the quality of the cultivation shelf is improved. Temperature regulation and control effect verification is intelligently fused with the mycelial growth speed conformity and the fruiting uniformity of the edible mushrooms, the growth conformity is quantified through image analysis, graded early warning is performed based on a secondary verification result, and a complete intelligent closed-loop control system is formed.

The invention belongs to the field of edible fungus culture, and particularly relates to a method for reducing disease occurrence and increasing the yield of dictyophora rubrovolvata. According to the method, before planting, a rotary cultivator is used for ploughing soil, wood vinegar is sprayed, temperature and humidity are controlled after hyphae are put on the soil, wood vinegar is sprayed in the growth stage of bamboo eggs, and temperature and humidity are controlled until harvesting, so that the occurrence of diseases is reduced, and the yield of dictyophora rubrovolvata is increased. The invention provides a method for reducing diseases and increasing the yield of dictyophora rubrovolvata through measures of improving the pH value of soil, preventing soil caking, stimulating rapid growth of hyphae, stimulating twisting of the hyphae, reducing diseases and insect pests and the like. By means of the method, use of pesticides in the dictyophora rubrovolvata planting process can be reduced, the yield of dictyophora rubrovolvata is increased, and dictyophora rubrovolvata diseases are reduced.

The invention discloses an edible fungus multi-parameter growth model construction and environment intelligent optimization method based on deep learning, and the method comprises the steps: collecting environment data, such as temperature, relative humidity, carbon dioxide concentration, illumination intensity, and matrix moisture content, and hypha and sporocarp images; the growth state index is constructed through weighted fusion of hypha respiration intensity, primordium density, pileus color gradient and other characteristics and is used for representing the growth health degree. And predicting a future growth trend based on an image and environment dual-channel feature fusion model, and obtaining prediction credibility according to a prediction error. An environment regulation and control strategy is established according to growth state improvement, energy consumption and physiological risks, equipment control instructions of ventilation, humidification, dehumidification, light supplement and the like are output, and dynamic optimization of a multi-parameter environment is achieved; and meanwhile, the model is updated online in combination with feedback data, so that the system has the capability of adapting to environment change and strain difference. The system can realize accurate monitoring and automatic regulation and control of the growth state of the edible mushrooms, and is suitable for industrialized production of the edible mushrooms.

The invention discloses an armillaria mellea liquid hypha powder liquid fermentation device which comprises a tank body and an electric control system, the upper part of the tank body is fixedly communicated with a feeding cylinder and an exhaust cylinder, the bottom of the tank body is fixedly communicated with a discharging cylinder and an air blowing pipe, and the feeding cylinder and the discharging cylinder are respectively fixedly provided with an electric valve for controlling whether the feeding cylinder and the discharging cylinder are conducted or not. A lifting mechanism is mounted at the top of the tank body, an electric driving rotating mechanism is mounted on the lifting mechanism, and a main stirring shaft and an auxiliary stirring shaft are driven by the lifting mechanism in a lifting manner. According to the stirring device, the first scraping strip and the third scraping strip are not in contact with the inner wall of the tank body in the stirring process through the elastic part and the lifting mechanism, and can be controllably in contact with the inner wall of the tank body in the cleaning process, and the inner wall of the tank body is cleaned under the driving of the electric driving rotating mechanism, so that the abrasion and the influence on materials are reduced, and the stirring efficiency is improved. And sterile air entering the tank body can be guided in the material stirring process by utilizing the upper flow guide cover, the lower flow guide cover, the stirring plate and the through hole.

The invention belongs to the technical field of agricultural control, and particularly relates to a pesticide for inhibiting fusarium graminearum as well as a preparation method and application thereof. The pesticide is a chitosan / chestnut flower polysaccharide composite material, and the chitosan / chestnut flower polysaccharide composite material is obtained by forming nucleophilic substitution between-COOH in chestnut flower polysaccharide and-NH2 in chitosan and generating amido bonds; the chestnut flower polysaccharide is obtained through a water extraction and alcohol precipitation method. As a pesticide, the chitosan / chestnut flower crude polysaccharide composite material has a good antibacterial rate, EC50 of fusarium graminearum hypha growth and spore germination are 442.6318 mu g / mL and 8.3516 mu g / mL respectively, in addition, the chitosan / chestnut flower crude polysaccharide composite material can promote germination of wheat seeds, the content of photosynthetic pigments in leaves is increased, and the yield of the wheat seeds is increased. And the activity of crop pathogenic bacterium defensive enzyme is improved.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof. The strain is separated from saline-alkali soil in the Uygur autonomous region in Xinjiang and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No.65485. By utilizing a response surface optimization method, the cellulase activity reaches 92.376 U / mL and is improved by 95.6% compared with that of a non-optimized cellulase activity; the growth inhibition rate on alternaria solani, alternaria alternate, fusarium oxysporum and botrytis cinerea hyphae is 88.47-91.08%, and the metabolite (especially an ethyl acetate extract) can significantly inhibit Korla pear diseases caused by alternaria alternate. The strain is resistant to high and low temperature, acid and alkali and salt stress, can degrade straw (the degradation rate is 21.9%), and provides a high-quality strain resource for resource utilization of agricultural wastes and biological control of plant fungal diseases.

The application discloses application of 5,8,11-eicosatrienoic acid in prevention and treatment of plant fungal diseases and belongs to the field of pesticide chemistry. The 5,8,11-eicosatrienoic acid mainly prevents and treats diseases caused by the oomycete class capsicum phytophthora, the compound is a long-chain unsaturated fatty acid, has an antifungal disease effect and can be used as a fungicide to prevent and treat plant diseases. The compound can effectively prevent pathogenic bacteria from invading plants by inhibiting mycelium growth, enhancing cell membrane permeability and interfering with the spore sac release process, and is further used for preventing and treating huge plant fungal diseases. Biological tests show that the compound has a significant concentration-dependent inhibitory effect on the mycelium growth of the capsicum phytophthora, and the half effective inhibitory concentration (EC50) is 60.2 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is more than 83%, the 5,8,11-eicosatrienoic acid can effectively inhibit the release of the capsicum phytophthora spore sac, and the EC50 is 156.3 µg / mL. At a concentration of 200 µg / mL, the inhibition rate is morethan 77%.

The invention discloses a fermentation material phellinus igniarius cultivation method, and relates to the technical field of phellinus igniarius cultivation.The phellinus igniarius cultivation method specifically comprises the following steps of compost preparation, composting fermentation, strain mixing, bagging spawn running and fruiting management.The invention further provides a culture medium bag for fermentation material phellinus igniarius cultivation. Sealed air bag strips are evenly distributed on the inner side of the packing material, a long-strip-shaped cushion material is arranged between the air bag strips, air holes are evenly distributed in the positions, corresponding to the cushion material, of the packing material, and after the packing material is filled with a fermentation phellinus igniarius culture base material, a fermentation material phellinus igniarius fungus stick is formed. A breathable growth groove used for mycelial growth is formed in the fermentation material yellow mulberry fungus stick; a lower cover body and an upper cover body are fixedly arranged at the two ends of the packing material respectively. The cost is reduced, nutrients are efficiently utilized, the air permeability is good, spawn running is accelerated, hypha activity is enhanced, the pollution rate is reduced, and the quality of sporocarp can be effectively improved.

The invention discloses a tough hypha structure and a culture method thereof. The tough hypha structure comprises a porous hollow support carrier, culture medium cloth and a hypha layer. The porous hollow support carrier comprises a support framework and a plurality of carrier holes penetrating through the support framework. The culture medium cloth is arranged on the porous hollow support carrier and comprises biomass nutrient fibers completely laid on the porous hollow support carrier and a plurality of fiber holes penetrating through the biomass nutrient fibers. The mycelium layer is arranged in the carrier holes and the fiber holes in a surrounding and penetrating mode, extends up and down and completely wraps the porous hollowed-out supporting carrier and the culture medium cloth, and the diameter of each carrier hole ranges from 10 micrometers to 1000 micrometers and is larger than that of each fiber hole. The invention further provides a culture method of the tough hypha structure. The porous hollow support carrier is used as a bottom support structure, so that the growth uniformity of mycelia can be improved. By adopting the porous hollow support carrier and the culture medium cloth, the flexibility, the structural strength and the ductility of the tough hypha structure can be improved.

The application discloses a bucket type cultivation method for overcoming continuous cropping obstacles of bamboo shoots. Through the bucket type inoculation, the mycelium is effectively isolated from the miscellaneous bacteria and pathogenic microorganisms in the soil before the mycelium completes the dominant expansion, the mycelium expansion stability is obviously improved, and the mycelium death and yield loss caused by the continuous cropping obstacles are avoided. Field tests show that the yield of the second year can be recovered to more than 90% of the yield of the first year by using the method, and the yield of the second year is less than 10% of the yield of the traditional reseeding mode, and almost no yield is obtained, so the method has good stability and generalizability.

The invention discloses a method for accurately identifying and screening morchella strains by using molecular markers, and belongs to the technical field of edible mushrooms. The method comprises the following steps: transferring a purified morchella strain to a culture medium for dark light cultivation; adding the cultured morchella hyphae into liquid nitrogen, grinding into powder, then adding a lysis solution, and carrying out lysis; adding modified magnetic beads into the lysate for adsorption, and then filtering, washing and eluting to obtain a DNA extracting solution; carrying out PCR (Polymerase Chain Reaction) amplification on the DNA; then carrying out high-resolution detection on an ISSR product; and then, carrying out functional SNP-KASP typing. According to the method, by fusing ISSR polymorphism analysis and a functional SNP-KASP typing technology, magnetic beads are used for adsorbing DNA to remove polysaccharide interference, and on the basis that the low cost advantage of traditional ISSR is reserved, three functional markers are completed, and accurate screening of yield, heat resistance and flavor is achieved.

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiA, in particular to a bacillus thuringiensis engineering bacterium HD73 delta cdsR (P5014-SPchiD-chiA) strain, and the preservation number of the bacillus thuringiensis engineering bacterium HD73 delta cdsR (P5014-SPchiD-chiA) strain is CGMCC (China General Microbiological Culture Collection Center) No.35677. The HD73 endogenous gene chiA gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-SPchiD-chiA), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with HD73 (P5014-SPchiD-chiA).

This application belongs to the fields of microbiology and cell biology, and provides a method for directly preparing germination-promoting fungal protoplasts from orchid protocorms, comprising: (1) physical homogenization; (2) filtration; (3) removal of plant contents and residual cell walls; (4) purification of mycelial clusters; and (5) release and purification of fungal protoplasts. Through the rational design of homogenization and purification steps, this application achieves high-yield, high-purity, and high-vitality preparation of germination-promoting fungal protoplasts, providing excellent materials for orchid gene variety breeding and genetic engineering operations.

The invention discloses a bactericide composition for preventing and treating earthen archaeological site mushroom diseases and application of the bactericide composition, triazole bactericides and methoxyl acrylate bactericides are used as active ingredients and compounded with a synergist, the mass ratio of triazole to azoxystrobin is 1: 1-1: 9, the content of the synergist is 0.01%-0.1% of the total mass, the content of the methoxyl acrylate bactericides is 0.01%-0.1% of the total mass, and the content of the methoxyl acrylate bactericides is 0.01%-0.1% of the total mass. The composition shows a remarkable synergistic effect, the FIC index is smaller than or equal to 0.5, and the composition has an efficient killing effect on spores, hyphae and sporocarps of common mushrooms in earthen ruins. The composition ensures safety to a ruins body material, shows a color difference value [delta] E < 3, a penetration depth greater than 5 cm, and maintains long-term stability in a humid environment for at least 12 months. The bactericide formula is systematically optimized for earthen ruins mushroom diseases for the first time, and various macro fungi including coprinus brachypoda, coprinus comatus, pleurotus ostreatus and pleurotus citrinopileatus are effectively prevented and treated. Through laboratory screening, synergistic effect testing, permeability and safety evaluation and field application verification, the composition has an excellent prevention and treatment effect.

The invention provides a wheat nitrogen fertilizer application reducing and effect increasing method, and belongs to the technical field of biological fertilizers. According to the method, under the background of straw returning to the field, arbuscular mycorrhizal fungi and mushroom residue and excrement decomposed fertilizer are combined as a main body, then peat soil and bentonite are added for wheat cultivation, and application reduction and efficiency improvement of wheat nitrogen fertilizer are achieved; wherein the arbuscular mycorrhizal fungi and the mushroom residue and excrement decomposed fertilizer are combined to promote soil straw degradation and assist nitrogen transfer, the peat soil and the bentonite can improve the water and fertilizer retention capacity of the soil and promote hypha growth and reproduction, available nitrogen in the soil is increased, soil nutrient loss is reduced, and the nutrient absorption capacity is enhanced; further, nitrogen fertilizer absorption and utilization of wheat are improved, and dependence on chemical fertilizer is reduced.

The application belongs to the technical field of microorganisms and particularly relates to a kind of Aspergillus ustus and application thereof.The Aspergillus ustus is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.40820.The Aspergillus ustus can be separated to obtain sesquiterpenes shown in formula I and formula II, and the sesquiterpenes have good bacteriostatic activity on Physarum polycephalum, and the bacteriostatic rates are 79.45% and 76.71% respectively at a final concentration of 100 μg / mL, which is equivalent to the control agent of bercyn in inhibiting mycelial growth.

The preparation method comprises the following steps: mixing a termite palatable cellulose material, wheat bran, gypsum, cane sugar and water to prepare a culture material, packaging and sterilizing the culture material, inoculating the Irpex lacteus, culturing until hyphae cover all the culture material, and carrying out freeze-drying to obtain the Irpex lacteus living fungus termite bait. The hypha living body state is kept for trapping odontotermes formosanus to eat. Experiments of three indoor devices show that the food calling effect of the Irpex lacteus bait block on odontotermes formosanus is obviously superior to that of a sterile culture material contrast bait block. Field tests show that the eating efficiency of the Irpex lacteus living fungus bait is obviously superior to that of natural plant bait circulating in the current market. Meanwhile, breathable tubular and bag-shaped packaging is convenient to store and carry, and hyphae can survive for a long time. The Irpex lacteus living fungus bait is good in termite trapping effect, high in eating speed, not prone to being polluted by infectious microbes and suitable for monitoring, trapping and killing odontotermes formosanus on dams and gardens.

The invention discloses a Ustilaginoidea virens inoculant, a preparation method and application of the Ustilaginoidea virens inoculant in Ustilaginoidea virens inoculation, and belongs to the technical field of agricultural microorganisms. According to the method, two-step shake-flask culture is combined with the steps of filtering, centrifuging and spore germination, so that the ustilaginoidea virens inoculum taking germinated spores (GC) as the core is prepared, and the problems of hypha aging, low spore activity, asynchronous infection and the like in the existing inoculation method are effectively solved. The inoculant has a tender hypha structure and strong infection activity, and the inoculation efficiency and the pathogenesis consistency are remarkably improved. When being used for preparing transcriptome samples, metabolome samples and other omics samples, the strain avoids non-specific immune interference caused by PAMPs released by broken hyphae, ensures synchronous infection process, and is real and reliable in immune response. The invention provides a stable and efficient inoculation system with a clean background for the research on the ustilaginoidea virens-rice interaction mechanism, and has a good application prospect.

The invention relates to a traditional Chinese medicinal material fungus morphology identification method and system, and relates to the field of traditional Chinese medicinal material quality detection.The method comprises the steps that marking is conducted according to a suspected fungus area to determine a hypha curve analysis position; acquiring local image information; analyzing local image information to determine a reference hypha curve graph; determining medicinal material fixing parameters based on the hypha curve analysis position, wherein the medicinal material fixing parameters comprise medicinal material fixing coordinates and medicinal material fixing angles; generating a small ball impact scheme based on the medicinal material fixed parameters, wherein the small ball impact scheme comprises an impact point and an impact speed; executing a small ball impact operation according to the small ball impact scheme, and determining a current hypha curve graph after the small ball impact operation; determining a hypha curve variable graph based on the current hypha curve graph and the reference hypha curve graph; and outputting hypha positions based on the hypha curve-shaped variable diagram. The method has the effects that collision textures and fungal filaments on the surfaces of traditional Chinese medicinal materials are distinguished, and the situation that the misjudgment rate is high due to the fact that physical damage and biological hyphae are difficult to distinguish through manual treatment is avoided.

A mycelium structure according to the present disclosure includes: a mycelium formed of hyphae connected in a three-dimensional shape; and starch covering the hyphae. A density of the mycelium structure is preferably 0.8 g / cm3 or more and 5.0 g / cm3 or less. An average diameter of the hyphae covered with the starch is preferably 2.0 μm or more and 20.0 μm or less. In a stress-strain curve indicating a relationship between a stress (MPa) applied by a tensile test and a strain (%) at that time, a maximum stress in an elastic region is preferably 2.0 MPa or more and 40.0 MPa or less, and a maximum strain is preferably 0.5% or more and 4.0% or less.

The invention discloses application of m-cresol to prevention and treatment of pepper southern blight, and belongs to the field of plant disease prevention and treatment. According to the invention, it is found for the first time that m-cresol has an inhibition effect on sclerotium rolfsii, wherein EC50 of m-cresol is 0.1560 [mu] L / mL; the m-cresol with the concentration of 2 * EC50 can completely inhibit sclerotium germination, sclerotium generation and hypha growth of the sclerotium rolfsii. Furthermore, m-cresol can be used for preventing and treating pepper southern blight caused by sclerotium rolfsii, the morbidity and disease index of pepper southern blight can be effectively reduced by using a m-cresol solution with the concentration of 0.125% to perform root irrigation treatment on pepper planted in pathogenic bacteria soil, the relative prevention and treatment effect reaches 100%, and adverse reaction of pepper plants cannot be caused. The invention provides a new method for controlling the pepper southern blight by using a medicament, and the method has the advantages of high efficiency, low cost, low environmental harm and the like.

The application discloses a genetic improvement experimental platform and method for a fungus in factory production of pilose antler mushroom, relates to the technical field of pilose antler mushroom production, and comprises a bottom plate, a rotating shaft rotatably connected to the top of the center position of the bottom plate, a wedge fixedly connected to the end of the rotating shaft away from the bottom plate, a groove with a size matched with that of an extruding piece and arranged around the center point of the wedge on the outside of the wedge, a rotating piece fixedly connected to the top of the center position of the wedge, a protrusion with a size matched with that of a rotating shaft and arranged on the rotating piece, and an extension rod rotatably connected to the top of the rotating piece. The nutrient substance inside the test tube is uniformly shaken to be evenly distributed in the test tube, the nutrient substance inside the test tube is quickly and fully mixed, the mycelium does not appear to have growth problems due to uneven distribution of the nutrient substance in the growth process, and thus accurate test results are obtained.