119 results about "Mother culture" patented technology

The invention relates to the field of microbial fermentation, in particular to a preparation method of a compound Daqu, so as to solve the problems of the traditional Daqu such as single nutritional components of raw materials, longer starter-making and brewing time, low utilization rate of grains, high cost and the like. The preparation method comprises the following steps: (1) treating the raw materials of the Daqu; (2) inoculating aspergillus niger 41254, aspergillus niger UV-11, monascus, mucor racemosus, actinomucor elegans, rhizopus oryzae, saccharomyces cerevisiae, candida drusei, acetobacter, bacillus subtilis and lactobacillus delbrueckii 6100 into a conical flask containing the raw materials of the Daqu for culturing, then expanding the culture and obtaining a mother culture; and(3) adopting uncooked materials for making a starter, inoculating the obtained mother culture and the raw materials of the Daqu into a solid fermentation device, keeping the temperature at 30 DEG C-33 DEG C, continuing for 30-33h, discharging the starter and finally carrying out low-temperature vacuum drying. The preparation method enriches the nutritional components in the raw materials and improves the quality of a brewed product; and a plurality of bacterial strain are artificially added, thereby improving the utilization rate of the grains, shortening the starter-making cycle, improving the yield of the brewed product and reducing the production cost.

The invention discloses a method for producing Black Tremellodon Gelatinosum belonging to the fungus cultivating field. The process of the invention includes preparation of mother culture, preparation of stock, preparation of cultivar and artificial cultivation. The invention is characterized in that the mother culture is cultivated and prepared by combining an encarpium mycelium of the Black Tremellodon Gelatinosum and a rhizoid mycelium of the Black Tremellodon Gelatinosum. Compared with the prior art, the method for producing Black Tremellodon Gelatinosum of the invention has filled the gap of the artificial cultivation technology of the Black Tremellodon Gelatinosum in the prior art and has the advantages of low cost, easy realization, etc. The invention can be broadly applied to the large-scale artificial production of the Black Tremellodon Gelatinosum.

The invention provides functional soy peptide fermented milk and a preparation method thereof and relates to a manufacturing method of fermented dairy products in the field of food processing. The method is carried out according to the following steps: (1) the preparation of fermented base stock; (2) the preparation of mother cultures; (3) synchronous enzymolysis and fermentation; (4) blending and homogenizing; (5) ultrahigh pressure sterilization and filling; by adopting the steps, a finished product is obtained. The method adopts protease and lactic acid bacteria synchronous-enzymolysis fermentation technology, and ultrasonic waves are adopted to process slurry-residue mixture which is ground, so as to increase the dissolving-out rate of the protein and prepare acidity soy-bean milk by ultrahigh pressure and non-heat processing. The product taste is coordinated without enzymolysis odour; the enzymolysis fermentation time can shorten to 2-4h, and the production cost is low. The ultrahigh pressure processing can remarkably improve the product taste; the method can keep and produce new functional peptide, the lactic acid bacteria is inhibited to ferment continuously, and a certain micro-organism viable counts are kept; the shelf life of the acidity soy-bean milk is prolonged, so as to prevent the acid milk from being whey-separated owning to continuous increasing of acidity; inaddition, the product can be stored and marketed at normal temperature.

The invention discloses a method for culturing edible fungi, which includes the following steps: culturing the mother culture in larger scale to prepare liquid culture spawn, introducing the liquid culture spawn together with culture medium which needs to be sterilized separately to cultivating material for thick solid ventilated culturing, when the cultivating material is filled with hypha, briquetting and shaping the cultivating material, and sending the briquetted cultivating material to a mushroom house to produce mushroom. The method is environment-friendly, energy-saving, short in production period, low in pollution rate and small in work intensity.

The invention relates to cultured Ganoderma yunnanense fruiting bodies and a culture method of the fruiting bodies. The culture method comprises the following steps: screening Ganoderma yunnanense spores and fruiting bodies obtained from field by single spore hybridization technique to obtain Ganoderma yunnanense fruiting strains; performing systematic breeding through tissue isolation of fruiting bodies; performing natural systematic breeding for 3-5 years to obtain Ganoderma yunnanense original mother cultures with strong stability, high metabolic capacity and high safety; and preparing mother cultures, preparing stock seeds and cultivated species, and culturing for one year with log, sawdust, wooddust and crop straws and other leftovers of agriculture and forestry industry as main culture raw materials, and a fruiting body activating agent, wheat bran, rice bran, corn meal, sugar and mineral elements as a culture medium in natural environment conditions while manually controlling temperature, humidity, air and illumination to obtain Ganoderma yunnanense fruiting bodies. The method of the invention is low in cost, easy to implement, can be widely applied for large-scale production of Ganoderma yunnanense, and realizes sustainable utilization of natural resources of Ganoderma yunnanense.

The invention discloses a method for realizing industrialization cultivation of lucid ganoderma, comprising mother culture, stock culture, cultivation bag manufacturing, inoculating, spawn running management, lucid ganoderma emergence management and harvesting. The method has the characteristics that the biological efficiency, sporocarp commodity rate and land use ratio are obviously improved and time and labour are saved.

The invention relates to a method for producing cooked pu-erh tea, which belongs to the technical field of pu-erh tea processing. The method is an improvement based on the prior method for processing the pu-erh tea. The improvement comprises the following steps: placing sundried green tea in a carton or a hemp bag, and placing the carton or the hemp bag on a warehouse platform 20 centimeters over the ground with moisture content within 13 percent; using a fermenting case for after-fermentation to realize complete off-ground clean production; evenly inoculating pu-erh tea fermented mother culture produced by a method of CN 2008102333700.X onto the sundried green tea in a spraying mode for pile fermentation; aspersing water on the sundried green tea with aspersion amount of between 30 and 35 percent for foods to make moisture content of the tea to be between 40 and 45 percent; carrying out the pile fermentation in the fermenting case for 35 to 40 days below 60 DEG C; and spreading a tea sample with a thickness of between 10 and 12 centimeters for natural drying after pile fermentation of the pu-erh tea, or drying the tea sample by mechanical equipment at a temperature below 60 DEG C. The method has the advantages of easy control of processing process, clean production environment, non pollution of the product, short time of pile fermentation and stable product quality, and can be widely used for large-scale production of the cooked pu-erh tea.

The invention relates to a method for producing rhizoma gastrodiae companion armillaria mellea through liquid submerged fermentation. The method is characterized in that a production technology of the rhizoma gastrodiae companion armillaria mellea through the liquid submerged fermentation comprises five steps as follows: (1) strain isolation of the armillaria mellea; (2) mother culture of the armillaria mellea; (3) preparation of solid seed bacteria; (4) liquid fermentation culture; and (5) cultivar inoculation and culture of the armillaria mellea. According to the method, various culture media of the armillaria mellea are provided, and problems that the armillaria mellea is rapid in degradation and slow in growth of strains, low in mycelium yield and varied in size and quality of the strains are solved; compared with traditional solid fermentation, the production technology has the advantages that the growth cycle of finished cultivar products of the armillaria mellea is greatly shortened, in addition, the rate of finished products is increased, and large-scale factory production of the high-quality rhizoma gastrodiae companion armillaria mellea is realized.

The invention discloses a ganoderma undergrowth bionics wild cultivation method. The method comprises the steps of A, choosing a wild ganoderma variety in the South China area and separating a mother culture; B, choosing a cut-log; C, cutting the cut-log into wood sections with the length of 20-30 cm; D, putting the cut-log into fungus bags; E, putting the bagged fungus material into a sterilization room; F, putting strains on an inoculation shelf and conducting space sterilization; G, when inoculation is conducted, pinching the strains into small blocks of 2-4 cm<3> with hands; H, choosing a room with good thermal insulation performance as a cultivation room, wherein the room temperature is at 25-28 DEG C; I, choosing a Chinese fir forest, a weed tree forest or a bamboo forest with the canopy density of 0.6-0.8, good ventilation and drainage performance and loose and fertile soil texture as a ganoderma cultivation plot; J, choosing Spring Festival to the end of the second month in lunar calendar as cultivation time; K, removing the bags and burying fungus sticks, wherein the burying density is 1500-3000 sticks/mu (a Chinese area unit and is equal to 666.67 m<3>). The ganoderma undergrowth bionics wild cultivation method mainly aims at undergrowth planting of ganoderma fungus sticks in the South China area, according to the method, no facilities and manual watering are needed, continuous harvest for many years can be achieved, and the ganoderma is high in yield and good in quality.

A method for culturing the Se-enriched sporophores of phellinus igniarius and extracting the selenopolyose from them includes such steps as preparing the mother culture medium from 13 raw materials including potato, agar, glucose, bean powder, etc, preparing stock culture medium from 14 raw materials including mulberry wood chip, wheat bran, corn flour, bean powder, etc, inoculating, culturing stock, culturing three-class spawn, preparing mycoplasm, and extracting selenopolyose. It can be used for preventing and treating cancer.

The invention discloses a formula for the culture medium of cordyceps militaris liquid strains and a method for culturing the same. The invention is characterized in that the method comprises the following steps: preparing a culture solution with the pH value being 7.5 from the following components by percentage: 0.8% of peptone, 2% of glucose, 0.1% of ammonium citrate, 0.1% of magnesium sulfate, 0.1% of monopotassium phosphate, 0.002% of vitamin B1 and 92.9% of water; filling containers, such as erlenmeyer flasks, saline bottles or preserving jars, with the culture solution; carrying out regular autoclaving on the culture solution; then, grafting the slant mother culture of cordyceps militaris onto the culture solution; and carrying out standing and culturing for 7 to 10 days in a light-blocking and airtight culture room with the temperature being 18 to 22 DEG C to obtain the cordyceps militaris liquid strains. The invention dispenses with fermentation tanks, shaking tables and other devices, therefore, the investment is small; no sterile air is introduced, therefore, the operation is convenient; and no energy is consumed, as a result, the invention is energy-saving and environment-friendly.

The invention discloses a method for cultivating oyster mushrooms. The method includes the following steps of mother culture making, stock culture making, preparing and applying of standard selenium liquid, cultivating culture making and mushroom producing management. According to the method for cultivating oyster mushrooms, inorganic selenium is biologically converted into organic selenium facilitating absorption of the human body; meanwhile, the content of the selenium of the oyster mushrooms is increased, the food-drug health caring value of the oyster mushrooms for people is increased, the yield is increased by about 30% on the basis of the original yield, the yield is increased, the enrichment amount of the selenium in every 100 g of fresh mushroom bodies can be 0.1-0.2 g, and the quality of the oyster mushrooms is improved.

The present invention discloses a detoxification agent for vomitoxin in a biodegradable feed and a preparation technology thereof. The detoxification agent comprises the following raw materials: 40-60 parts of bentonite, 20 parts of immune polysaccharides, 10-20 parts of a free radical scavenger, and 20-30 parts of a vomitoxin degrading bacillus subtilis powder agent. A preparation method of the bacillus subtilis powder agent comprises the following steps: 1) original strains are subjected to a seed liquid activation and a mother culture is conducted in a primary fermentation tank loaded with a culture medium; 2) a mother culture liquid is subjected to an amplification culture in a second fermentation tank loaded with a LB culture medium; and 3) the bacterium body and the fermentation liquid are separated, the separated fermentation liquid is concentrated, and the concentrated fermentation liquid is dried to prepare the vomitoxin degrading bacillus subtilis powder agent; and the powder agent is combined with the two raw material mixtures in the above parts by weight to obtain the finished products. The detoxification agent has the degradation rate of the vomitoxin at 95% or more, is high in degradation efficiency, and avoids the defects of adsorbents. Besides, the immune polysaccharides and free radical scavenging components are added in the products, so that the detoxification agent can play the functions of protecting liver and improving the immunity of body, and is obvious in effects.

The invention discloses a method for preparing organic bacteria liquid, the organic bacteria liquid prepared by the method, and an application of the organic bacteria liquid. The organic bacteria liquid is prepared by breeding bacteria from industrial and agricultural wastes which are rich in organic materials, such as plant ash, furfural residue, excrement, activated sludge, and crop straw, and screening, expanding and propagating the bred bacteria. The organic bacteria liquid has the characteristic of high bacterial activity, and can be used as a mother culture in the production of a microbial fertilizer or directly used as a seed-dressing agent.

The invention provides a manufacturing method for morchella mother culture. The method is characterized by comprising the following steps of: 1, boiling 1 to 3 parts of fresh insect-free populus bonatii smashed branches or populus bonatii leaves in 10 parts of water based on a mass ratio for 25 to 30 minutes, filtering the mixture, and taking juice to prepare populus bonatii leachate; 2, mixing the populus bonatii leachate and PDA culture solution in a mass ratio of 1: 1, adding 0.2 percent of Na3PO4.12H2O, 0.2 percent of KH2PO4, 0.05 percent of MgSO4 and 0.005 percent of VB1 based on the mass percent of the mixed liquor, uniformly stirring the mixture, and sterilizing the mixture at the temperature of 121 +/1 DEG C for 30 minutes to prepare a culture medium; and 3, cooling the culture medium, inoculating the morchella mother culture, culturing the inoculated culture medium at the constant temperature of 23 DEG C for 10 to 12 days, and culturing the culture medium in an incubator at the temperature of between 8 and 12 DEG C for 4 to 6 days. The manufacturing method has the advantages of strong hypha, strong twisting force, and easy formation of sclerotium on protospecies or cultivar.

The invention discloses a preparation method of an edible mushroom wood plant mother culture medium and a product thereof. The preparation method comprises the steps of: 1, with deciduous species wood as raw materials, processing the deciduous species wood into a wood plant; 2, soaking the wood plate in a nutrient solution with nutrients required by the growth of the hypha, fishing out and then dripping water on the surface until no water drops exists; and 3, loading the wood plant prepared in the step 2 into a high-temperature resistant glass bottle, filling in gaps with compost, covering and flattening with the compost, plugging cotton balls, and sterilizing to obtain the edible mushroom wood plant mother culture. The edible mushroom wood plant mother culture has a long storage period and ageing resistance, and can be stored for 3-5 months at a normal temperature of 5-25 DEG C, thereby improving the culture preservation effect by 10 times. The edible mushroom mother seed cultured bythe culture medium has the advantages of vigorous hypha, high survival rate, strong vitality and rapid hypha development.

The invention relates to a technology for cultivating edible mushroom, in particular to a method for cultivating straw mushroom by applying liquid strain. The method comprises the following steps of: (1) composting and fermenting a cultivating raw material; (2) sub-packaging the cultivating raw material in a cultivating basket for sterilization, moving the cultivating raw material into a cultivating room, and arranging the cultivating raw material on a shelf; (3) configuring a liquid culture medium, and inoculating a straw mushroom mother culture for cultivating after sterilization; (4) uniformly pouring the cultivating raw material into the straw mushroom liquid strain after the cultivating raw material is cooled, and mixing the straw mushroom liquid strain and the cultivating raw material; and (5) fruiting management and collecting. Compared with the common solid strain cultivating method which is used at present, the method for cultivating the straw mushroom has the advantages of quick seed production, environment production and easiness in finding potential pollution condition, the mushroom production ability is considerable, and the method for cultivating the straw mushroom is suitable for a large scale industrial production.

The invention discloses a potato dregs fermentation energy fodder which can replace bran and a preparation method thereof. The principal raw material of the fodder is potato dregs which is prepared through zymohydrolysis and mixed culture solid-state fermentation by adding accessories of bran, urea, ammonium sulphate, monosodium orthophosphate and bitter salt; the preparation method comprises: preprocessing of material, zymohydrolysis of pectase and cellulase, expanding propagation of strains of production, preparation of liquid seed, mother culture and leaven, fermentation and parching; the invention has the advantages that: the potato dregs fodder prepared through fermentation by adopting the method has the content of crude protein of more than 18 percent, over sized fibres of less than10 percent and ash content of less than 5 percent, the potato dregs fodder can meet the standard of first level wheat bran according to <GB 10368-89 wheat bran for fodder>, has no solanine or other microelements with toxic effects, is rich in multiple Probiotic bacteria and has good flavor, and can be used as energy fodder for fowl and Livestock instead of bran.

A cultivation method of selenium-enriched Cordyceps militaris mother fungi belongs to the technical field of the cultivation of Cordyceps militaris. The cultivation method comprises the following steps: 1) cultivating selenium-enriched strains on a test tube slant; 2) cultivating selenium-enriched Cordyceps militaris liquid strains; and 3) cultivating the selenium-enriched Cordyceps militaris. The cultivation method uses organic selenium yeast which has the lowest inhibitory effect on the growth of Cordyceps militaris, thus the selenium content of the culture medium is up to 50mg/L, the selenium content of the cultivated Cordyceps militaris is up to 35-50mg/kg which is triple of the selenium content of Cordyceps militaris with additional inorganic selenium, and the yield is increased by 20%.

The invention provides a method for producing the liquid strain of cordyceps militaris. The method comprises the following steps: (1) inoculating the mother culture of cordyceps militaris to a dedicated strain culture medium to carry out the shaking culture; (2) moving the strain solution cultured in step (1) into a dedicated strain culture medium to carry out the amplification culture, wherein the inoculation amount accounts for 2% to 20% of the volume of the culture medium; (3) filtering the strain solution cultured in step (2) by using a sieve with the bore diameter thereof being 50 to 500 meshes to obtain the filtrate containing more than 107 conidia per ml; and (4) diluting the filtrate obtained in step (3) by using sterile water until the density thereof is 105 to 107 conidia per ml to obtain the liquid strain of cordyceps militaris. The method of the invention is applicable to inoculating and cultivating the fruit body of cordyceps militaris, wherein the dedicated strain culture medium contains carbon source, yeast nitrogen base, magnesium sulfate, monopotassium phosphate, dipotassium phosphate and agar.

The invention relates to a cultivation method of mushrooms, in particular to a manual bag cultivation process for phellinus igniarius. The process comprises the following steps: (1) purifying strains separated from wild phellinus igniarius bodies, and inoculating the purified strains into a mother culture medium for culturing to obtain a phellinus igniarius mother culture, inoculating the phellinus igniarius mother culture onto a stock culture medium for culturing to obtain a phellinus igniarius stock culture, and inoculating the phellinus igniarius stock culture onto a cultivated species culture medium for culturing to obtain phellinus igniarius cultivating species; (2) inoculating the phellinus igniarius cultivating species into bacteria sticks for performing ganoderma emergence culturing; (3) performing ganoderma emergence management. Proved by a large amount of culturing practice, the manual bag cultivation process has the advantages that true manual cultivation of phellinus igniarius can be realized, and the size of a single fruit body can be controlled manually according to opening sizes; through manual bag cultivation of the phellinus igniarius, wild rare resources and hardly-regenerative resources are manually regenerated in a short time.

The invention relates to a cultivation method for fungi, especially to an artificial bag cultivation process for Phellinus igniarius. The process comprises the following steps: (1) purifying a strain isolated from wild Phellinus igniarius sporocarp, then inoculating the purified strain to a mother culture medium for cultivation so as to obtain a mother Phellinus igniarius strain, inoculating the mother Phellinus igniarius strain to a stock culture medium for cultivation so as to obtain a Phellinus igniarius stock and inoculating the Phellinus igniarius stock to a cultivar culture medium for cultivation so as to obtain a Phellinus igniarius cultivar; (2) inoculating the Phellinus igniarius cultivar to a mushroom-stick and carrying out cultivation for Ganoderma germination; and (3) managing germinated Ganoderma. According to results of considerable cultivation practice, it is proved that the artificial bag cultivation process for Phellinus igniarius in the invention can realize artificial cultivation of real Phellinus igniarius; moreover, the size of individual sporocarp can be artificially controlled by controlling the size of an opening. Through artificial bag cultivation of Phellinus igniarius, artificial short-term regeneration of wild rare resources and resources needing a long regeneration period is realized.

The invention relates to a method for rapidly producing Pu-Er ripe tea, which belongs to the technical field of Pu-Er tea processing. Based on the traditional Pu-Er tea processing method, the method comprises the following improved steps: fermented mother culture, fermented native tea, glucose or sucrose is selected as a fermentation substrate and activated; activation liquid is evenly inoculated to sun-withered primary tea; the fermentation substrate after being activated is diluted to 10 times and blanched, and water which is in accordance with national food water sanitary standards or drinking water sanitary standards is selected as blanched water; the water content in the tea is finally ensured to be 40-45 percent; the fermented piling time is 30-35 days, and the piling temperature is controlled to be below 60 DEG C; piling tea is spread and naturally withered or mechanically dried after the requirements are achieved, and the drying temperature is less than 60 DEG C. Compared with the traditional method, the invention has the advantages that the quality of the Pu-Er ripe tea is more stable, the processing time is shortened by 10-20 percent, the production cost is reduced, the efficiency is improved, the process is standard, and the operation is simple, and can be widely used for large-scale and standardized production of the Pu-Er ripe tea.

The present invention provides a health-care medicine composition and its preparation method. Said method includes the following steps: making photosynthetic bacterial pool, Chinese herbal medicine liquor, mineral solution and honey undergo the process of biological fermentation, then making them be combined with lactic acid microbial pool, yeast microbial pool, Cram-positive actinomycetes bacterial pool and filamentous microbial pool of fermentation line to form five family microbial community to obtain mother culture, then making said mother culture and nucleic acid, vitamin, SOD and amino acid undergo the process of biological emulsification so as to obtain the invented composition. Said composition has extensive application in preparation of medicine for curing broad-spectrum bacterial disease, broad-spectrum viral disease, damaged gene and diseases due to damaged cell, and specially for preventing and curing SARS.