42 results about "Rhizoid" patented technology

The invention discloses a chaetomium globosum FSR-74. The collection unit of the chaetomium globosum FSR-74 is China General Microbiological Culture Collection Center, the collection date of the chaetomium globosum FSR-74 is September 19, 2014 and the collection number of the chaetomium globosum FSR-74 is CGMCC No. 9690. The strain is relatively slow in growth and has light brown colonies and an irregular wave-like edge when subjected to plate culture at 25 DEG C in a potato dextrose agar (PDA) culture medium. FSR-74 ascocarps are supergene and fixed on the surface of a matrix by rhizoids; top hair is wavy, brown and has intervals, and dense wart points are arranged on the surface; 8 ascospores are arranged in an ascus; the ascospores are shaped like lemons and have single apical bud holes. The chaetomium globosum FSR-74 has an efficient broad-spectrum bacteriostatic effect against main pathogenic bacteria causing ginseng rust rot, blight, sclerotinia rot, rust rot, blackspot, sheath blight and gray mold. The invention further provides a biological control mechanism and application of the chaetomium globosum FSR-74 in prevention and treatment of plant fungal diseases, as well as application in preparation of a microbial agent for preventing and treating the plant fungal diseases.

The invention provides a long stem grape caulerpa industrial cultivating method. The long stem grape caulerpa industrial cultivating method includes the following steps that step A, rhizoid of long stem grape caulerpa to be cultivated is implanted in a massive adherence made of polymeric sponge, and it is guaranteed that a cut end of the rhizoid is wrapped in the middle of the polymeric sponge; step B, light ray of a sea grape cultivating factory is adjusted to 2500-3500 lux through a light ray regulation control system; step C, the water depth of a cultivating pool is not lower than 1.8 m, the water temperature ranges from 28 DEG C to 30 DEG C, and the growth of caulerpa body is guaranteed; step D, nutritive salt is obtained after accurate processing and purification are performed on sea water for cultivating, and the nutritional requirement of the growth of the caulerpa body is guaranteed. The long stem grape caulerpa industrial cultivating method uses eutrophic sea water to cultivate the sea grape, the discharged waste water is actually sea water cleaner than cultivating water, the cultivating method by adopting the cultivating pool achieves land cultivation of the long stem grape caulerpa, a large number of sea areas are saved, and therefore the cultivating process of the long stem grape caulerpa is convenient to manage.

The invention relates to a method for soilless planting of mosses on a net shed sand bed in a solar greenhouse, and belongs to the technical field of moss planting. According to the technical scheme, a disease and insect pest control system is arranged in the solar greenhouse; a suspended sandy planting bed and a built-in sun-shading net tent are built in the solar greenhouse, wild mosses attached to the surface of a shady moist stony rock mass are collected, and moss seedlings are cultivated on a seedbed of a three-layer composite structure; the moss seedlings are taken out and smashed into moss pulp, and the moss pulp is applied on gauze flat laid on the surface of the suspended sandy planting bed to cultivate a lawn-shaped moss gametophyte colony. The innovative moss soilless planting technology has the advantages that industrialized production of the mosses is achieved by utilizing the solar greenhouse with a regulable environment in the North, a produced lawn-shaped moss gametophyte rhizoid intertwining layer is thick, tight and not easy to loosen, the leaves are bright green, the market selling price is high, and an ecological green environment-friendly moss decoration material is utilized sustainably and widely.

The invention relates to a transformation system for Spirodelapolyrrhiza callus induction and capable of realizing transgene stable inheritance, and belongs to the field of biotechnology. The method is as below: first, inducing the formation of Spirodelapolyrrhiza callus through different plant culture medium and plant hormone combination by using the high dedifferentiation ability of new rhizoid meristematic zone or a binding site of new rhizoid and thallus meristematic zone; and then using Agrobacterium or particle bombardment method to realize integration of exogenous DNA fragments into Spirodelapolyrrhiza genome and stable inheritance of the transformed exogenous DNA fragments in Spirodelapolyrrhiza. The system of the invention can integrate any exogenous DNA fragment to Spirodelapolyrrhiza genome. The technology requires short time, has high efficiency, can induce callus in 2 weeks, and reaches an induction rate of 100%; and transgenic plants can be obtained in the shortest time of about 3 months.

The invention relates to the rearing of economic brown algae, in particular to a preservation method of fertilized eggs and embryos of sargassum fusiforme, which specifically comprises the following steps: culturing male and female parents of sargassum fusiforme under the conditions that the light intensity is 10000-50000 lux, the photoperiod is 12-16 hours, and the temperature is 18-24 DEG C, replacing water within 24 hours once, and culturing for 7-10 days, so that the parents synchronously discharge eggs and sperms; and collecting the fertilized eggs within 12 to 24 hours after the parents synchronously discharge the eggs and the sperms, completing the collection of the fertilized eggs and the embryos at a temperature of 0 to 8 DEG C within 8-16 hours after fertilization, and then preserving. The invention utilizes the low-temperature method to culture the eggs in an appropriate culture medium within 8-16 hours after the sargassum fusiforme eggs are fertilized, and then preserves the fertilized eggs and the embryos, thereby substantially delaying growth process, so that the cross-regional transport is completed before rhizoids are not formed so as to carry out seed rearing.

The invention discloses a sargassum fusiforme rhizoid summer-cross seedling-raising device comprising positioning piles, pile cables and positioning cables. The positioning piles are fixed on four points, the positioning cables are connected on the positioning piles through the pile cables, such that a square structure is formed; rope cables are fixed on the positioning cables; a position-control cable is arranged in parallel between each two rope cables; and the rope cables and the position-control cables are arranged in a same direction with tides. Float balls are tied on the rope cables, such that the rope cables are positioned on surface water; weights are tied on the position-control cables, such that the position-control cables are positioned in shallow water. Therefore, stereo V shapes are formed. Two ends of seedling ropes are respectively tied on the rope cables and the position-control cables, wherein an included angle formed by two seedling ropes is 135-150 DEG. When the device is used for raising seedling, the seedling ropes are in stereo V shapes, and are distributed in different water levels, such that a well-spaced layout is formed, and the damage caused by siganus which is regularly active in a same water level can be effectively prevented. With the device, a stereo grid structure is established, and siganus damages can be prevented. Under the impact of wind and waves, stable culturing facility and structure can be maintained, and seedling growth and maintenance time can be ensured.

The invention discloses a method for repairing an intertidal sargasso field. Sargasso seedlings are transplanted and cultivated by adopting a net bag seedling bundling and stone casting method, so that the sargasso seedlings can be subjected to sexual reproduction and asexual reproduction; the net bag seedling bundling and stone casting method comprises the following steps: (a) the sargasso seedlings are fixed in a net bag, and rhizoids of the sargasso seedlings are clung to the net bag; (b) stone is put into the net bag, and the opening of the net bag is fastened and sealed; (c) in a low tide, the net bag obtained in the step b is cast between a low water line of a neap tide and that of a spring tide in an intertidal zone. According to the method, the operation is convenient, a huge matrix does not need to be built, and the consumed fund is little.

The invention provides a rapid cultivation method for a vitis sinocinerea caulerpa reproductive shoot. The rapid cultivation method comprises the following steps: cutting a stolon of 8-10cm as a reproduction mother twig from an end part of a grape caulerpa stolon; burying the reproduction mother twig into sand; covering the shade of a cultivation area with black sunshade net, keeping the light intensity of the cultivation area lower than 1000 lux, keeping the water temperature of the cultivation area to be 28-30 DEG C, and feeding shellfish with plankton feeding habit; applying phosphorus-containing nutritive salt, and when the rhizoid length is greater than 18cm, turning over the sand to collect rhizoid; inserting the collected rhizoid into sand, keeping the light intensity be 1500-2500 lux, and cultivating for 100-120 hours at the water temperature of 25-28 DEG C. By the adoption of the technical scheme of the invention, the vitis sinocinerea caulerpa reproductive shoot can be rapidly cultivated by using an artificial system, and the growth rate and the unit area yield are increased.

A cultivation method of a collybia albuminosa mother strain comprises the following steps that firstly, fruiting bodies of collybia albuminosa are subjected to tissue separation under aseptic conditions, and fruiting body mycelia are obtained after separation; secondly, rhizoid of collybia albuminosa is subjected to tissue separation, and rhizoid mycelia are obtained after separation; thirdly, thefruiting body mycelia are transferred into a test tube containing a culture medium for cultivation, after the mycelia grow to 1.3-1.8 cm, the mycelia are inoculated into the rhizoid mycelia, and cultivation continues to obtain the mature mother strain; or the fruiting body mycelia are transferred into a triangular flask containing the culture medium for shake cultivation, the rhizoid mycelia areinoculated when a small quantity of mycelium pellets suspend in liquid, cultivation continues, and the liquid mother strain is obtained after a large quantity of small mycelium pellets suspend in theliquid. One of the two modes is selected for cultivation. The mycelia biomass of the mother strain cultivated through the cultivation method is 2.3 g/100 ml or above, the mycelia are dense and strong,and the color of the mycelia is a little amber.

The invention discloses a seedling method of ulvales green algae asexual propagation, comprising the following steps: wild algae body is divided into segments below threshold value (often less than 1-5mm); the segments are tissue cultured; after segment cells grow larvae with rhizoid again, the rhizoid and the larvae are stripped from fixed matrix and cultured in a aerating way after being grinded; and the tissues of the rhizoid and the larvae are induced to have facultative parthenogenesis so as to release swarm cells or fixed cells and germinate to form a new plant. Therefore, the method reaches the augmentation of clone and is used for producing seeds. The invention has outstanding advantages of short seedling production period, no limit by seasons, stable hereditary character and low production cost.

The invention discloses a high-disease-resistance tissue culture seedling raising method for baby cabbages. The high-disease-resistance tissue culture seedling raising method comprises the following steps: (1) material taking, (2) cultivation, (3) differentiation, (4) rooting and strong seedling culture, (5) multiplication culture, (6) rooting culture and (7) temporary planting: exercising rooted test-tube seedlings in a greenhouse for 5-7 d, washing a culture medium left on roots with water, and temporarily planting the seedlings in a rhizoid substrate; and after 15-20 days, new roots grow out and baby cabbagerooting seedlings are obtained. The high-disease-resistance tissue culture seedling raising method improves disease resistance of baby cabbages, can effectively resist infection of the baby cabbages by pathogenic microorganisms, enhances a disease resistance advantage of the baby cabbages, effectively increases a planting survival rate and improves economic benefits.

The invention belongs to the field of aquarium ornaments, and particularly relates to a method for manufacturing red and green grape algae landscaping stones. The method includes: evenly binding red grape algae and green grape algae from wild or markets on movable stones with the same size to form algae stones; regularly and quantitatively adding a desired nutriment solution and monitoring the water quality; adjusting a suitable illumination condition and a suitable water quality condition; removing binding ropes till the red grape algae and green grape algae come up filiform rhizoid which is attached on the movable stones; and regularly trimming and maintaining the stones. The method is high in operability, is low in cost, and is suitable for large-scale production, can enrich sea aquarium landscaping products, can stabilize the sea aquarium life-support system, can improve the ornamental value of sea aquarium landscaping tanks, has significant meaning for development of the ornamental value of the red grape algae and the green grape algae, and can play a certain push role in sea ornamental fishery.

The invention provides a rapid and convenient sheet preparing method of compact filamentous bacteria and relates to the technical field of filamentous bacteria sheet preparation. The method comprises the following steps: scribing one line perpendicular to the surface of a culture medium and near the edge of bacterial colony of the filamentous bacteria in a culture dish by a surgical knife blade, scribing an arc line along the outer side of the edge of bacterial colony of the filamentous bacteria, and cutting of a small culture object with width being 1 to 2 mm together with agar; stirring up the culture object slightly with the surgical knife, and flatly paving the cut agar surface on a glass slide to enable the knife cutting surface to abut against the glass slide; performing microscopic examination from low power to high power. The sheet preparing method provided by the invention is rapid and convenient; long-time cultivation is not needed; the original mycelial morphology is completely not damaged, the original and natural growth state of the filamentous bacteria is maintained, the method is particularly suitable for typical for observation of substrate mycelium such as spore hypha, aerial hyphae, rhizoid and stolon of compact filamentous bacteria (comprising undeveloped filamentous bacteria such as actinomycetes and aerial hyphae), and the morphological characteristics of different growth stages can be observed simultaneously.

The invention provides a novel method for propagating liverwort. According to the method, the liverwort is propagated by using a liquid culture medium; a plastic bracket is arranged in the liquid culture medium, so that tissue blocks of the liverwort cannot be immersed in the culture medium; and the tissue blocks of the liverwort are managed respectively before or after the rhizoids grow, and therefore, the aim of the rapid and efficient propagation of the liverwort is achieved.

The invention discloses a cultivation method of moss for biological monitoring, which comprises the following steps: collecting a whole piece of moss, cutting off the rhizoid of the moss, sieving the collected whole piece of moss for the first time, and sowing for the first time; cutting off rhizoid with moss after first-time sowing, performing second-time sieving, and preparing for second-time sowing; a moss culture solution is prepared, the moss culture solution is used for conducting second-time sowing and cultivation on moss, and protonemata is obtained; preparing a moss attachment matrix and an attachment matrix skeleton; the protonemata obtained in the second step is sown and attached to a moss attachment substrate, the protonemata differentiates on the moss attachment substrate to generate gametophytes, and the gametophytes grow on the moss attachment substrate to form target moss. The moss for biological monitoring cultivated by the invention has high sensitivity to micro-plastics in the environment, can effectively monitor the micro-plastics in the environment, and can be used as a preferred environmental micro-plastic pollution indication plant.

The invention discloses an in-vitro preservation method for sargassum fusiforme germplasm resources, and mainly relates to a low-temperature and low-salinity preservation method taking sargassum fusiforme rhizoid as a germplasm preservation material. The method is characterized in that long-time in-vitro preservation of the sargassum fusiforme rhizoid under the conditions that the temperature is 8-10 DEG C and the salinity is 19-20%, and the growth rate and the germination rate of the sargassum fusiforme rhizoid in the preservation period are effectively inhibited. The preservation method is easy to operate, resources are saved, and survival and germination of the sargassum fusiforme rhizoid are not affected; besides, normal growth, germination and seedling formation of the sargassum fusiforme rhizoid can be recovered under the proper culture condition, so that the key problem that sargassum fusiforme indoor germplasm resources are difficult to preserve is effectively solved, the preservation time can reach 4 months or above, the rhizoid germination rate can reach 400% or above after recovery culture, and the seedling rate can reach 150% or above.

The invention discloses a making method of a modular moss wall. The making method includes steps: making module base trays, building water-retaining matrix layers, paving nutritional matrix layers, making moss layers, fixing the moss layers, and mounting the module base trays. The moss wall is formed by connecting multiple module base trays already filled with the water-retaining matrix layers, the nutritional matrix layers and the moss layers with multiple module fixing frames fixedly connected on a wall body through a sleeving connection structure. The module base trays are safely and stablyconnected on the wall body through the sleeving connection structure, so that mounting and demounting are convenient; after the moss wall is formed, modules exist independently, and demounting of a single module has no impact on other modules, so that maintaining is convenient. Each moss layer is flexible in fixing mode and good in fixing effect, so that moss is less prone to shedding; each nutritional matrix layer can be adhered to rhizoid gaps of the moss to become a binder between the corresponding moss layer and the corresponding water-retaining matrix layer to enable rhizoid of the mossto be closely combined with the corresponding water-retaining matrix layer, and the method is especially suitable for being used to make moss walls by using naturally collected cushion tufted mosss.

The invention discloses a method for sterilizing a turbinaria conoides rhizoid explant. The method is characterized by comprising the steps of clearing attachment such as impurity algae on the explant by utilizing a brush and adopting the turbinaria conoides rhizoid as an explant for the tissue culture, placing the explant into 1% to 5% antibiotic to be soaked for 5h to 8h, then taking out the explant, immersing the explant in a potassium iodide solution for 2min to 5min, taking out the explant, sterilizing the explant in povidone iodine for 1min to 3min, and finally washing the explant by utilizing the cooled and filtered high-pressure sterilized seawater. By adopting the method, the application concentration and the application time of the antibiotic can be reduced, and the drug resistance of the explant can be avoided; meanwhile, through the sterilizing effect of the potassium iodide and povidone iodine, not only can the bacteria or fungus on the surface and inside the explant be killed, but also the unrecoverable damage of the sterilizing agent to the explant can be avoided, and 85% bacteria-free feasible rhizoid explant can be obtained through the method.

To provide a vegetation sheet which requires no soil as a base of cultivation of Sunagoke grown seedlings and enables the cultivation of Sunagoke grown seedlings in a stable state even in a severe environment such as on a steep slope or in a strong wind. A vegetation sheet 1-1 comprises a surface layer 10 in which Sunagoke grown seedlings 30 are laid and a core layer 20 which allows rhizoids 30a of the Sunagoke grown seedlings 30 extending with growth to enter downward therein. The core layer 20 has a rough netlike structure which has spaces having an aperture size equal to or larger than the thickness of each rhizoid 30a so as to allow the rhizoids 30a to enter therethrough.

The invention discloses a method for building a sargassum bed in an artificial riprap shoreline by adopting an adhered algal reef. The method for building the sargassum bed in the artificial riprap shoreline by adopting the adhered algal reef comprises the following steps: (1) collecting sargassum seedlings, with complete forms, with rhizoid attached onto shells on intertidal zone rock; (2) selecting stone, and fixing the shells to which the sargassum seedlings are attached to surfaces of the stones with an adhesive to produce the algal reef; (3) placing the algal reef in middle and low intertidal zones of the riprap shoreline, making the algal reef fixed among riprap, ensuring that the sargassum seedlings on the algal reef face upwards or are arranged towards waves, and conducting sea area cultivation; and (4) conducting sea area cultivation to make the rhizoid of the sargassum seedlings ramble and attached onto the surfaces of the stone for the second time, and conducting vegetativepropagation to regenerate a large amount of new seedlings to make sargassum reproduce on the riprap shoreline, so as to achieve building of the sargassum bed. According to the method, by adopting theproduction method of the adhered algal reef, the natural sargassum seedlings are transplanted into the artificial riprap shoreline area, the method is easy for large-area application and popularization, and is convenient to operate and low in cost, and the labor intensity is low.