163 results about "Mercury Bichloride" patented technology

The invention discloses a separation and culture method using ginseng cambium stem cells. The separation and culture method using ginseng cambium stem cells is characterized by comprising the following steps: (1) firstly performing disinfection treatment on the root tubers with the diameter of 2 cm of northeast ginseng by 0.1% mercury bichloride for 10 minutes, and then transversely cutting the northeast ginseng into semicircular slices with the thickness of 0.2 cm and containing periderms, phloems and cambiums; (2) placing the semicircular slices on a WPM (woody plant medium) containing 0.1-4.0 mg / L of picloram and 0.1-4.0 mg / L of 2,4-dichlorphenoxyacetic acid, and culturing at 25 DEG C in the dark; (3) taking out the obviously-multiplied explants of the cambiums after two weeks, separating the cambium stem cells, transferring the cambium stem cells on a subculture medium and culturing, wherein the subculture medium is a WPM containing 0.1-4.0 mg / L of picloram and 0.1-4.0 mg / L of 2,4-dichlorphenoxyacetic acid, and subculture is performed every two weeks. According to the method, separation and culture for ginseng stem cells are performed by using the unsplit cambium stem cells, and the cambium stem cells are infinitely multiplied, so that production can be effectively expanded.

The invention belongs to the technical field of plant tissue culture and particularly relates to a method for building a bamboo reed tissue culture system. The method comprises the steps of (1) explant disinfection, wherein axillary buds of bamboo reeds are fully soaked in 75% of ethanol for 10 seconds and then moved into 0.2% of mercury bichloride solution for 10 minutes; (2) primary culture, wherein a culture medium is formed by adding cane sugar 30g / L and agar 8g / L in MS, then adding 6-BA 5mg / L and IBA 0.5mg / L and finally adjusting potential of hydrogen (pH) to 5.8; (3) secondary culture, wherein a culture medium and culture conditions of the secondary culture are the same as the culture medium and culture conditions for the primary culture; (4) strong bud and root culture, wherein the buds are cultured in the secondary culture medium for one month to finish strong bud culture and then planted in a rooting culture medium, and the rooting culture medium is formed by adding IBA 0.5 mg / L into the MS; and (5) domestication and transplantation, wherein the domestication is performed by using a floating bed domestication system for one week and then transplantation is performed. By means of the method, the efficient breeding of the bamboo reeds can be achieved.

The present invention belongs to the field of plant tissue culture, and specifically relates to a tissue culture and rapid propagation method of white crane lily. Sterilized with 0.1% mercuric chloride, ②cut the stems into 1-2mm thick slices, and put them together with the terminal buds in the induction and differentiation medium supplemented with 6‑BA and VC for culture, ③callus or adventitious buds were inoculated Add 6‑BA and IAA to the medium, wherein the adventitious bud mass used to induce rooting seedlings is directly inserted into the medium added 6‑BA, IAA and TIBA, ④Single buds with plant height ≥30mm or Three clumps with a main bud plant height ≥ 25mm were inserted into the rooting medium supplemented with activated carbon. The invention provides a method for tissue culture and rapid propagation of white crane lily, which can obtain a large amount of asexually propagated white crane lily tissue culture seedlings in a short time.

The invention discloses a cinnamomum camphora tissue culture method. The method comprises the following steps: picking new sprouted semi-lignified branches of cinnamomum camphora in the same year in April to May each year, scissoring branches with 2-3 leaves, removing the leaves, oscillating and washing in sterile water for 3-5 minutes, treating by using 70% alcohol for 1-2 minutes, placing in 0.1% mercury bichloride for 6-10 minutes, and cleaning by using sterile water for 3 times; inoculating the treated semi-lignified branches into an adventitious bud induction medium, thereby obtaining an aseptic explant, culturing under illumination conditions, and thus forming callus and adventitious buds for expanding propagation; culturing in a mulitiplication expanding propagation culture medium and a root induction medium, and cultivating to obtain rooting seedlings; and transplanting the cultured cinnamomum camphora rooting seedlings, wherein the survival rate is over 95 percent.

The invention discloses a construction method for a vaccinium ashei tissue culture rapid propagation system and relates to a plant tissue culture method. According to the invention, a stem of a water culture seedling is selected as an explant, after being treated by ethyl alcohol and mercury bichloride, the stem is inoculated to a stem shoot system induction culture medium and then is vertically inserted into stem shoot system multiplication culture, in the rooting culture, the concentration of IBA is 2.0 mg L<-1>, and the rooting percentage is greater than 85%. According to the invention, the water culture seedling is used as the source, and the contamination rate and the browning rate are obviously lower than that of the greenhouse culture seedling; the ethyl alcohol disinfection and the mercury bichloride are used for disinfection to mutually promote each other; the stem shoot system induction and the multiplication are consistent with an orthogonal experiment result; the influence of IBA on rooting reflects the result of a single-factor randomized block experiment.

The invention discloses a method for recycling mercury from mercury-containing solid wastes. The method comprises the following steps: introducing N2 (or other inert gases) into mercury-containing wastes at 100 DEG C-200 DEG C and drying for 0.5-10 hours; introducing the chlorine at 200 DEG C-400 DEG C for chlorinating for 0.5-10 hours; introducing N2 again at 400 DEG C-750 DEG C and dry distilling for 1-10 hours; and lastly, washing with a solvent, thereby obtaining a product, and meanwhile, absorbing the dry distilling tail gas by using absorption liquid. According to the method, the recovery rate of the mercury can reach 80-99%; the recycled waste mercury bichloride contact agent carrier is not damaged; the mercury content is extremely reduced to below 0.1% and the recycled waste mercury bichloride contact agent carrier still can be applied to the production of a mercury bichloride contact agent; the pollution problem of the mercury-containing waste and the recycling problem of the mercury resource are solved according to the method; the traditional mercury recycling method and the mercury bichloride production are integrated; the production cost is saved; and the method has the advantages of zero remnant of waste residue and waste water, simpleness in technology, low cost, waste recycling and environmental protection.

The invention discloses a teapot dates tissue culturing method. The method comprises the steps of clipping teapot dates with a bud stem tip in a large land; cleaning the teapot dates with tap water; putting the teapot dates into alcohol to sterilize; sterilizing by using mercury bichloride; cleaning the teapot dates by using sterile water; inoculating into a starting culture medium; transferring to a meristem culture medium; transferring to a successive transfer culture medium; and finally, finishing the establishment of the whole tissue culture system in a root medium so that the teapot dates tissue is subjected to sterile culture and transplantation under a manually-controlled illumination and temperature condition. In-vitro rapid propagation of the teapot dates is carried out according to the embodiment of the invention, the process from incubation to rooting needs 70 days, the teapot dates tissue can be transplanted to a seedling culture bed on the ground, the induction survival rate is up to 97 percent, the increment reaches 10 times, and the transplantation rooting rate is also up to 95 percent.

The invention provides a tissue culturing and rapid propagating method for chloranthy florida var. plena. The tissue culturing and rapid propagating method comprises the following steps of: (1) washing stem tips of caulicles of chloranthy florida var. plena with sterile water on an ultra-clean table, immersing the washed stem tips by 75% of alcohol for 20-30 seconds, immersing and disinfecting the stem tips by 0.1%-0.2% of mercury bichloride for 6-10 minutes, and inoculating the stem tips, which are washed by the sterile water, into a cluster bud induction medium for primary induction culturing; (2) dividing cluster buds obtained through primary induction culture into small individual buds, and inoculating the small individual buds into a cluster bud propagation culture medium for subculturing; and (3) inoculating bud seedlings into a strong seedling rooting culture medium for rooting culturing when the bud seedlings grow to 2cm-3cm. The method provided by the invention can be used for rapidly propagating chloranthy florida var. plena, not only can save costs for culturing seedlings and improve production efficiency, but also is not restricted by external conditions, can be carried out in four seasons, saves seedling occupied area, can form a great amount of excellent test-tube seedlings in a short time, and can be used for large-scale and industrialized production.

The invention discloses a tissue culture method of red-leaf sacred bamboo, which belongs to the technical field of plant cultivation. The method comprises the following steps of: (1) selecting a healthy plant with typical ornamental character performance to serve as a material from red-leaf sacred bamboo seedling plants, cutting new sprouts and removing pinnately compound leaves in the morning ofa sunny day, and cleaning for later use; (2) soaking, disinfecting and flushing a cleaned branch with an alcohol solution and a mercury bichloride solution in sequence on an ultra-clean workbench; (3) stripping a stem apex in the branch, cutting a tip part and inoculating into a culture medium; (4) culturing for a certain period of time and transferring a plurality of indefinite buds formed by differentiation and proliferation of the stem apex into an enrichment medium for culturing; and (5) culturing for a certain period of time, cutting immature stems growing on the indefinite buds and culturing on a rooting medium to form a robust tissue culture rooted seedling. The tissue culture method has the advantages of high survival rate, high reproduction speed, multiplication coefficient of upto 7.3 and rooting rate of up to 87 percent. The obtained red-leaf sacred bamboo has high quality and wide market prospect.

The invention discloses a rapid propagation method of ginger virus-free seedlings by one step. The rapid propagation method comprises the following steps: A, selecting tender shoots germinating on ginger rhizomes as explants; B, carrying out heat treatment to the explants, so as to detoxify the explants; C, disinfecting the detoxified explants with ethyl alcohol and mercury bichloride; D, peeling stem tips by a dissecting microscope; E, inducing the stem tips to grow micro ginger cluster buds under light condition and constant temperature condition; F, carrying out virus detection on the micro ginger cluster buds by an enzyme-linked immunosorbent assay (ELISA); G, carrying out subculture multiplication culture on the virus-free micro ginger cluster buds; H, transplanting virus-free micro ginger cluster buds with roots after plant division. By the utilization of the rapid propagation method, various viruses and germs in ginger bodies are removed effectively, germ-free and virus-free ginger seedlings are cultured, virus-free healthy cultivation of the ginger is realized, space occupation of propagation is small, propagation speed is high, one virus-free bud can propagate more than hundred thousand virus-free ginger seedlings for one year, a problem of virus-free ginger seedling supplying in plantation of ginger is solved effectively, good quality of the ginger is recovered, the disease occurrence is reduced, and the output and quality of the ginger are improved.

The invention belongs to the technical field of plant biology, in particular relates to a method of tissue culture of Medicago sativa L. By way of developing a novel tissue culture technical method, the invention focuses on improvement and innovation of two technical nodes (seed disinfection and callus formation and differentiation) in culture steps. With adoption of a mercury bichloride and hydrogen peroxide compound disinfection method, the invention realizes the purposes of thorough disinfection effect and low pollution rate and minimum damage to seeds. Finally, the invention solves the problem of severe pollution to current explants. Meanwhile, a method of TDZ and 6-BA is adopted, thereby, the callus induction rate and the formation rate are remarkably increased, the callus quality is improved, the differentiation capacity of the callus is reserved to a maximum extent, the formation of embryoid sprouts is promoted so that the novel technical method of the tissue culture of the Medicago sativa L. which is efficient, saves time, and has low toxicity and low browning, is obtained.

The invention relates to the technical field of natural forest protection and recovery, in particular to a method of cultivating landscaping trees by wild-to-artificial cultivation of Choerospondias axillaris. The method includes the steps of a, preparing a nursery and a cutting bed; b, selecting cuttings; c, pruning and treating the cuttings, to be specific, pruning cuttings, and treating the cuttings; d, performing cuttage; e, performing post-cuttage management, to be specific, managing temperature and humidity, applying fertilizers, removing weeds, and controlling disease and pest damage; f, transplanting saplings. The method is characterized in that the cuttings are selected from the male trees of Choerospondias axillaris, which are tall, straight from top to bottom, in leafy profusion and attractive in appearance and which grow for 8 to 10 years; the cuttings are selected from late May to early middle of June, after successive five sunny days, at 8 to 10 AM; the cuttings are treated with 70% ethyl alcohol for 20s and soaked in 0.1% mercury bichloride for 8min, shaking is performed nonstop, and the cuttings are washed five times with sterile water. The method has the advantages that the cuttings are all from male plants of Choerospondias axillaris and Genetic endowment of germplasm resources of wild Choerospondias axillaris can be better guaranteed.

The invention provides a winged euonymus stem adventitious bud inducing method. The winged euonymus stem adventitious bud inducing method comprises the following steps that an annual branch good in growth vigor with the length of 3-5 internodes is selected, and a stem with leaves pruned off is obtained; a washing powder solution and flow water are used for washing the stem clean; an explant is sterilized on an ultra-clean worktable with a series of sterilization methods such as ultraviolet lamp irradiation, and 75% alcohol and Tween in combination with mercury bichloride; the stem is vertically inserted into a culture medium with the base part of the stem facing downwards; the culture temperature is set to be 24+ / -1 DEG C, the humidity is set to be 50-60%, the light period is 16 h of illumination, and the light intensity is set to be 2000-3000 lx; growth conditions are recorded. According to the winged euonymus stem adventitious bud inducing method, with the stem of winged euonymus serving as the explant, a winged euonymus stem sterile system is successfully established, and adventitious buds re successfully induced; besides, the inducing rate of adventitious buds reaches 70.67%, the average number of buds of each explant reaches 5.6, the propagation coefficient in the subsequent propagation process reaches 23.51, and the average bud length is 1.28 cm. The production cycle is short, and the propagation coefficient is high.

The invention provides antistatic rubber material and a preparation method thereof, wherein the antistatic rubber material contains antistatic agent of 3 to 5 weight percent with reference to rubber matrix of 100 weight percent; the antistatic agent is a mixture and comprises compositions according to the following weight ratio: emulsifying agent: polar solvent: organic amine: metal salt is equal to 3:3:1-2:1-2; and the metal salt is selected from lead acetate, mercury bichloride, copper sulphate, iron chloride, antimonic chloride, stannic chloride or silver nitrate. The rubber material prepared by the antistatic agent has excellent antistatic performance, surface resistivity reaching 10<6> ohm to 10<8> ohm as well as high-efficient and persistent static resistance; moreover, the rubber material has ideal compatibility with other additives of rubber products, thereby meeting the requirements of high temperature during manufacturing the rubber products without influencing the physical property and the chemical property of one another.

The invention discloses a tissue-culture rapid propagation method for succulents. The method comprises the following steps: (1) selecting and sterilizing an explant: cutting tender leaves or stems of the succulents, and washing the cut leaves or stems cleanly; carrying out sterilizing treatment by adopting spirit and mercury bichloride, so as to obtain the explant; (2) carrying out primary culture: inoculating the explant obtained in the step (1) to a primary culture medium for primary culture until buds are differentiated from the explant; (3) carrying out propagation culture: cutting off the buds differentiated from the explant in the step (2), and inoculating the buds to a propagation culture medium for propagation culture until the length of a new bud germinated from each growth point is 3cm to 5cm and the multiplication coefficient is greater than 2; (4) carrying out rooting culture: separating new buds germinated in the step (3) into single plants, and inoculating the single plants to a rooting culture medium for rooting culture until each single plant grows 2 to 3 new roots and the length of each new root is greater than 3cm. According to the method, the culture time is short, the propagation frequency is high, young seedlings are regular, and the labor cost is reduced, so that the method can be used for carrying out industrialized production.

The invention discloses a quick cultivation method for rhizoma bletillae tissue culture seedlings. The method comprises the following steps: selecting intact non-cracked rhizoma bletillae capsules and disinfecting the capsules by 75% alcohol for 30 seconds; then soaking and disinfecting the capsules by using 0.2% mercury bichloride for 10 minutes; washing 6 times by sterile water; fully and thoroughly washing, airing and uniformly spreading on a culture medium to cultivate; and when the seeds germinate to obtain seedlings which are about 2cm long, inoculating 3-4 seedlings as a cluster to a strong sprout culture medium to be cultured. Compared with the conventional tissue culture seedlings obtained by sterile germination of rhizoma bletillae seeds, the method just needs subculture once, so that the time consumed is short and at least three months are saved.

The invention discloses a yam seedling in-vitro propagation method comprising the steps of A, taking a yam tender stem with an axillary bud as an explant, and washing with sterile water after the alcohol and mercury bichloride surface is sterilized; B, cutting the stem into stem sections with joints on germ-free paper by using a sterilizing scalpel, and vertically inserting the stem sections into an induction culture medium in a polarity direction; C, culturing at constant temperature under light illumination, and growing cluster buds; D, cutting the cluster buds into a plurality of small cluster buds, and transferring into the induction culture medium for multiplication culture; and E, transferring robust seedlings into a rooting culture medium in a subculture process; opening a bottle and carrying out plantlet hardening when the seedlings are rooted, and then transplanting the seedlings into the medium after washing off; keeping temperature and moisture; and transplanting after the tissue culture seedling survives. The obtained yam seedling has the advantages of good hereditary stability, small propagation occupied space and high propagation speed, so that more than ten thousands of the yamn seedlings can be propagated by one bud for one year. The problem of supplying good-quality yam seedlings in yarn plant can be effectively solved. The established production system is stable, the period is shorter, and the scale quick propagation and production can be satisfied.

The invention discloses a blueberry tissue culture method. The blueberry tissue culture method comprises the steps that annual blueberry shoots are taken, 3-4 cm stem segments with buds are cut, are sterilized with ethyl alcohol with the concentration of 70-75% by volume for 10-30 seconds, then are sterilized with mercury bichloride with the concentration of 0.08-0.12% by volume for 6-8 minutes and are flushed for standby application; the stem segments with buds are cut into single stem segments with buds and with the length of 1-2 cm; the lower ends of the single stem segments are inserted into a primary culture medium for primary culture, and then multiplication culture and rooting culture are performed; by clearly and reasonably setting the hormone contents in the primary culture medium, a multiplication culture medium and a rooting culture medium and conducting reasonable sterilization processing before single stem segment inoculation, blueberry tissue culture is high in survival rate and is easy to succeed.

The invention discloses a rapid propagation method of Pinellia ternata stems. The method comprises the steps of: A, selection and induction of explants: selecting expanded petiole as explants, flushing by tap water, adding alcohol, soaking, draining alcohol, washing with sterile water, soaked with bromogeramine, draining the bromogeramine, washing with sterile water, adding mercury bichloride and Tween, soaking, washing with sterile water, inoculating to an induction medium, and culturing to form spheroids with buds; B, proliferation of spheroid buds: inducing to obtain spheroids with buds, cutting the buds in an ultra-clean bench, wherein the cut spheroids contain buds, and conducting proliferation culture; and C, the formation of seed stem: after proliferation culture, taking off single buds, inoculating into a seed stem medium in the ultra-clean bench, and prompting seed stem increase. The method is feasible and easy to operate, can effectively reduce the effects of endophyte on Pinellia ternata sterile culture, improve the efficiency of tissue culture and rapid propagation, and reach high growth efficiency.

The invention discloses a method for improving the embryogenesis efficiency and the plant regeneration efficiency of a stem nodule mustard microspore embryo. The method comprises the following steps of: (1) selecting materials, namely observing flower buds by a microscope, and selecting the flower buds with the diameters of 2-3 mm; (2) performing sterilization, namely performing sterilization by adopting 0.1 percent of mercury bichloride for 12 min, and washing the flower buds by germfree water for three times; (3) extracting pollens, namely adding a B5-13 liquid culture medium for grinding and filtration, and performing centrifugation; (4) performing heat-activation treatment, namely transferring sedimented pollens into an NLN-16 liquid culture medium containing a colchicine solution, culturing the pollens in the dark at the temperature of 32 DEG C for 48-52 hours; (5) performing induction to obtain the embryo, namely performing centrifugation, adding the NLN-13 liquid culture medium, packaging the pollens into a culture dish according to a unit of 1.5 flower buds per culture dish, and culturing the flower buds in the dark at the temperature of 25 DEG C; and (6) performing induction to obtain seedlings, namely after the embryo is formed, transferring the embryo into a B5-3 solid culture medium for culture. The invention creates a novel method of a stem nodule mustard free microspore culture technology and has a wide application prospect in breeding of stem nodule mustards.

The invention relates to the field of biotechnology application and discloses a rapid seedling propagation method for Chinese aralis. The method comprises the following specific steps: 1, washing a top dormant bud of Chinese aralis, which is taken as an explants, by using running water, soaking by using 70 percent alcohol, disinfecting in a 0.1 percent mercury bichloride solution, and finally thoroughly rinsing by using sterile water; 2, cutting the disinfected explant into small blocks of 0.5-1cm, inoculating to a prepared induction medium for cultivation, and differentiating adventitious buds, wherein the culture conditions are as follows: the light intensity is 1500-20001x, the temperature is 25+ / -1 DEG C and the illumination is 12 hours; 3, transferring the induced adventitious buds to a subculture medium for performing enrichment culture, so that bud seedlings are healthy and strong; and 4, inoculating the differentiated adventitious buds into a rooting medium, and transplanting when the adventitious roots grow out, wherein the survival rate of the transplanted seedlings can be over 80 percent. The method disclosed by the invention is easy and feasible to operate, high in propagation speed and suitable for industrialized seedling production.

The invention relates to a method for fast obtaining a regenerated plant for cayenne pepper anther culture. The method comprises the following steps: selecting an anther with microspores in a late uninucleate stage; positioning the anther at a low temperature of 3-5 DEG C for pretreatment which lasts 24-72 h; sterilizing a flower bud subject to the low temperature pretreatment with 75% of alcohol and 0.1% of mercury bichloride; cleaning the flower bud with sterile water; stripping the anther from the flower bud in a sterile condition; inoculating the anther in a culture medium comprising MS basic culture medium, 0.5-1.0 mg / L of NAA, 1.0-2.0mg / L of KT, 2 wt% of maltose, 0.8 wt% of agar and 0.5 wt% of activated carbon; carrying out heat shock treatment lasting for 6-10 days at a temperature of 33-37 DEG C; and continuing the culture at an illumination intensity of 2000 lx, an illumination time lasting 12 h and a room temperature of 25 DEG C till a complete plant with roots, sprouts and leaves is formed. The method can accomplish the formation of embryoids with large anthers and different types on one culture medium and obtain the regenerated plant. 7 weeks is needed in the process from the beginning of the anther culture to the fast obtaining of the regenerated plant. The method can be used for the case that haploids, dihaploids and other materials are obtained from cayenne pepper by means of the anther culture.

The invention discloses a tissue culture disinfection and sterilization formula of ormosia henryi prain and a tissue culture method of the ormosia henryi prain. Seed seedling stems are disinfected by 70% ethanol for 30 seconds, sterilized by 0.1% mercury bichloride for 8 minutes, disinfected by the 70% ethanol for 30 seconds and treated by 0.5ml / L of a plant tissue culture antibacterial agent to achieve the best disinfection effect; the optimal collection time of seedling explants is April; the stems are immersed by the 70% ethanol for 30 seconds, immersed by a mixed solution (containing 50mg / L rifampicin and 50mg / L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 8 minutes to achieve the best disinfection effect; and leaves are immersed by the 70% ethanol for 30 seconds, immersed by the mixed solution (containing 50mg / L rifampicin and 50mg / L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 6 minutes to achieve the best disinfection effect. Leaves of grown plants are relatively suitable for collecting in April, and are immersed by the 70% ethanol for 30 seconds, immersed by the mixed solution (containing 50mg / L rifampicin and 50mg / L chloramphenicol) for 30 minutes and immersed by 0.1% mercury bichloride for 12 minutes. According to the tissue culture disinfection and sterilization formula and the tissue culture method, seed seedlings, seedlings and grown plant tissues of ormosia henryi prain explants are disinfected by different disinfection methods, and the relatively good survival rate can be obtained; and sources of ormosia henryi prain tissue culture multiplication materials are greatly expanded, and the tissue culture effect of the ormosia henryi prain can be greatly improved by controlling disinfection time.

The invention belongs to the technical field of medicinal herb propagation and discloses a cremastra appendiculata seed tissue culture and propagation method. The method comprises the following steps:flushing with running water for 10 minutes, and stripping seeds; disinfecting by using 0.1% of mercury bichloride 10min+70% of alcohol 10s; inoculating into MS+2, 4-D1mg / L+BA 1mg / L callus induction;transferring into 1 / 2MS+NAA 1mg / L+BA 1mg / L callus to realize seedling differentiation; culturing under the conditions of 24+ / -1 DEG C and 2000-2100lx, and illuminating 8-10 hours per day; and placingculture bottles of tissue culture seedlings under room-temperature natural lighting conditions for hardening seedlings, cleaning with clean water, planting into a pre-disinfected substrate, and splitcharging by a feeding block. The induction rate can reach 55% within 50 days, the seedlings are grown within 40 days after culture, the survival rate of the seedlings is 83%, the new roots grow earlier, the root system is developed and strong, and plant growth is good.

The invention discloses a method for rapidly propagating Camelia sinensis cv. Chongqing pipacha. The method comprises the following steps of: sequentially cleaning an explant of a stem segment with buds of an indoor cutting seedling of the Camelia sinensis cv. Chongqing pipacha by using soapy water, soaking in carbendazim, and washing by using running water, completely absorbing the moisture on the surface of the explant, and removing small leaves from outer layers of the buds and the stem segment; sequentially disinfecting the explant by using alcohol and mercury bichloride under aseptic conditions, washing by using sterile water, inoculating into a Murashige and Skoog (MS) culture medium, culturing, and screening out a survival aseptic material; transferring the aseptic material into a startup culture medium, culturing and inducing into buds; and transferring the germinant aseptic material into an enrichment culture medium, and continuing to culture to obtain cluster buds with the average multiplication coefficient of 3.31 to 4.86. By the method, the aseptic material of which the sterilization survival rate is up to 88.5 percent can be obtained, the germination time is short, the multiplication rate is high, the cluster buds of which the average multiplication coefficient is up to 4.86 can be obtained within three months, and excellent characters of a genotype and a phenotype of the original clone can be maintained.

The invention relates to an iris germanica Jinwawa tissue culture method. The iris germanica Jinwawa tissue culture method comprises the following steps of 1, selecting a raw material of an iris germanica Jinwawa seedling growing on the land, cutting off young buds with stalks when the young buds are formed between early-March to mid-April, removing outer bracts of the young buds, and flushing by clear water for next use, 2, carrying out immersion disinfection of the cleaned young buds on a superclean workbench orderly by an alcohol solution and a mercury bichloride solution, and flushing by sterile water, 3, removing inner bracts located at lower parts of the young buds, removing top parts and ovaries of the young buds, and introducing the stalks located at insertion parts of the inner bracts into a medium, 4, culturing for a period of time, and when adventitious buds are formed on the insertion parts of the inner bracts, transferring the adventitious buds to a multiplication medium for culture, and 5, culturing for a period of time, cutting off cluster buds formed from the adventitious buds, and culturing the cut cluster buds in a rooting medium to form strong tissue cultured rooting seedlings. The iris germanica Jinwawa tissue culture method has the advantages of simpleness of disinfection, high survival rate and fast breeding speed.

The invention provides a treatment method of rhizoma bletillae seeds. The method comprises the following steps: 1) shearing to open rhizoma bletillae capsules, storing the rhizoma bletillae seeds into a tea bag, and then sealing an opening of the tea bag; 2) transferring the sealed tea bag with the rhizoma bletillae seeds, obtained in step 1), into a sterilized bottle on a super clean bench, adding 75% ethyl alcohol until covering the tea bag, vibrating and shaking for 30 to 60 seconds, transferring the tea bag into another sterile bottle, then adding 0.2% mercury bichloride disinfectant until covering the tea bag, fully vibrating and shaking for 8 to 12 minutes, and finally transferring the tea bag into sterile water to rinse for six to eight times; 3) absorbing water of the tea bag rinsed in step 2) through filter paper, and fully drying with air on the super clean bench. With the adoption of the treatment method, the pollution rate of the rhizoma bletillae seeds can be reduced, and moreover, the use rate of the seeds with broken capsules can be increased; in addition, the disinfecting effect is good, and the germination rate of the rhizoma bletillae seeds can be increased.

The invention discloses a tissue culture and rapid propagation method of ribbon grass. The tissue culture and rapid propagation method of the ribbon grass comprises the following steps of cleaning up underyearling and tender stem sections of the ribbon grass; obtaining an explant after sterilization treatment by adopting alcohol and mercury bichloride; inoculating the explant onto a starting medium to culture, and cutting off an axillary bud obtained through culturing and inoculating onto an initial medium to culture; taking a ribbon grass seedling obtained through culturing as a female parent, cutting, then inoculating onto a subculture medium to culture until growing to a complete plant, wherein the starting medium, the initial medium and the subculture medium are formed by 1 / 2 of DKW minimal medium, supplement of 0.5 to 1.0mg / L of TDZ, 0.06 to 0.15mg / L of NAA, 10 to 50 g / L of cane sugar, and 2 to 10 g / L of agar; finally washing away the culture medium from the obtained grew well tissue culture rooting seedling, and then directly transplanting into a greenhouseseed bed to propagate. The tissue culture and rapid propagation method provided by the invention is adopted to propagate the ribbon grass, is simple and convenient in culture process, and simple and easy to operate, and has the characteristics of high propagation coefficient, short propagation period and high survival rate.

The invention discloses a breeding method of Chinese black olive cutting seedlings. The breeding method comprises the following steps that 1, newly growing-out twigs of more than 10 years old are selected as cutting collection objects; 2, cuttings are processed, the collected cuttings are soaked in a nutrient solution for 30 minutes after being disinfected with a mercury bichloride solution, thenare dried in the shade and are stored at the temperature of 4-5 DEG C; 3, the cuttings are taken out during cuttage, are placed at the temperature of 25-30 DEG C for 2 hours and then are put in a dormancy breaking solution for 1 hour; 4, a cutting seedling matrix is prepared; 5, the cuttings subjected to dormancy breakage is inserted into the cutting seedling matrix; 6, water and fertilizer management and disease prevention are conducted on the cuttings, and transplantation with soil is performed when the number of roots of each cutting is more than 10 and the average root length is greater than 7 cm. The problem is solved by adopting the method that at present, no mature Chinese black olive breeding method is provided and large-range popularization is difficult. The cutting breeding method makes the survival rate reach 90% or above, makes the survival rate of transplantation after outplanting reach 86% or above and has the great value for continuation of precious species.