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A technique for tissue culture and teapot jujube, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of difficulty in popularization and low survival rate of teapot jujube tissue culture seedlings, etc.
Inactive Publication Date: 2013-07-10
BEIJING FORESTRY UNIVERSITY
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However, the survival rate of teapot jujube tissue culture seedlings obtained by conventional tissue culture methods is low, and it is difficult to popularize and use them on a large scale.
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[0022] In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, specific embodiments will be described in detail below. Unless otherwise specified, the technical means used in the examples are conventional means well known to those of ordinary skill in the art, and the raw materials used are all commercially available items.
[0023] The teapot jujube tissue culture method that the embodiment of the present invention provides, comprises the following steps:
[0024] Cut off the stem tips of Daejeon teapot jujube buds, wash them with tap water, sterilize them in alcohol, sterilize them with mercuric chloride, rinse them with sterile water, inoculate them on the starting medium, transfer them to the meristematic medium, and then transfer them to To the subculture medium, and finally complete the establishment of the entire tissue culture system in the rooting medium. Aseptically cultured and transplanted under ar...
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Abstract
The invention discloses a teapot dates tissue culturing method. The method comprises the steps of clipping teapot dates with a bud stem tip in a large land; cleaning the teapot dates with tap water; putting the teapot dates into alcohol to sterilize; sterilizing by using mercury bichloride; cleaning the teapot dates by using sterile water; inoculating into a starting culture medium; transferring to a meristem culture medium; transferring to a successive transfer culture medium; and finally, finishing the establishment of the whole tissue culture system in a root medium so that the teapot dates tissue is subjected to sterile culture and transplantation under a manually-controlled illumination and temperature condition. In-vitro rapid propagation of the teapot dates is carried out according to the embodiment of the invention, the process from incubation to rooting needs 70 days, the teapot dates tissue can be transplanted to a seedling culture bed on the ground, the induction survival rate is up to 97 percent, the increment reaches 10 times, and the transplantation rooting rate is also up to 95 percent.
Description
technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for obtaining teapot jujube tissue culture seedlings through the teapot jujube aseptic short branch cutting method. Background technique: [0002] Jujube (Ziziphus jujube Mill) is a plant of the genus Ziziphus Mill in the family Rhamnaceae, and it is a unique economic tree species native to my country. Jujube trees have strong adaptability, drought resistance, salt and alkali resistance, barren resistance, and good water and soil conservation. The fruit is very rich in sugar, Vc, cAMP, protein, fat, Vp, B vitamins, Fe, P, Ca, Zn and other essential substances for humans, and has high nutritional and therapeutic value. [0003] There are many kinds of jujube trees, highly heterozygous, complex genetic background, small flowers, low fruit setting rate, early embryo abortion, etc., resulting in difficulties in emasculation and artificial pollination, and it is difficult to ...
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